ライフサイエンスコーパス: multienzyme

1 constructing useful synthetic assembly-line multienzymes.

2 provide substantial evidence implicating the multienzyme aminoacyl-tRNA synthetase (mARS) complex and

3 New multienzyme amperometric biosensors are presented here w

4 n nature, we developed an efficient two-step multienzyme approach for the synthesis of a series of GD

5 We have used a novel multienzyme approach to generate a set of highly represe

6 nslocated to the appropriate site on the PKS multienzyme are located at the N-terminal region of the

7 These large multienzymes are organized into a series of functional u

8 The applicability of the new multienzyme assay to wine samples is illustrated.

9 synthases (PKSs) are a family of homologous multienzyme assemblies that catalyze the biosynthesis of

10 Editosomes are megadalton multienzyme assemblies that provide a catalytic surface

11 regulate cell division often proceed through multienzyme assemblies within defined intracellular comp

12 respiratory chain complexes can arrange into multienzyme assemblies, so-called supercomplexes.

13 types of active sites within this family of multienzyme assemblies.

14 he pyruvate dehydrogenase complex (PDC) is a multienzyme assembly that converts pyruvate to acetyl-Co

15 The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnov

16 assembly of conotoxins is a highly regulated multienzyme-assisted process.

17 integrate the kinase catalytic chassis into multienzyme-based regulatory networks.

18 This multienzyme bidirectional helicase-primase complex can p

19 ucts produced on modular polyketide synthase multienzymes by an assembly-line process in which each m

20 s rapid and room-temperature digestions with multienzyme capabilities.

21 xidase, and highlights the potential of this multienzyme cascade for the efficient synthesis of chira

22 ized in one-pot conditions by using biochemo multienzyme cascade of lipase M and tyrosinase.

23 Herein we report the application of a multienzyme cascade, generated in a single bacterial who

24 ic quantities of the amine donor or deploy a multienzyme cascade.

25 The immobilization of the multienzymes cascade on electroactive lignin nanoparticl

26 lity to combine multicomponent chemistry and multienzymes cascade transformations in a unique reactiv

27 ds that organize the spatial arrangements of multienzyme cascades with control over their relative di

28 ssembly of a cyclase complex or even a large multienzyme catalytic center.

29 The multienzyme catalytic phosphorylation of phosphatidylino

30 hydrolase into the bacterial cellulosome, a multienzyme cellulolytic complex, via its interaction wi

31 Clostridium cellulovorans produces a multienzyme cellulose-degrading complex called the cellu

32 cus flavefaciens produces a highly organized multienzyme cellulosome complex that plays a key role in

33 idly solubilizes cellulose with the aid of a multienzyme cellulosome complex.

34 dules play a crucial role in the assembly of multienzyme cellulosome complexes.

35 fungal and bacterial cellulase systems, the multienzyme cellulosome system of the anaerobic, cellulo

36 sible chaperone in the assembly of CynD or a multienzyme CNO complex.

37 scherichia coli 1-lip pyruvate dehydrogenase multienzyme complex (1-lip PDHc) with the C259N and C259

38 branched chain alpha-ketoacid dehydrogenase multienzyme complex (approximately 4-5 x 10(3) kDa) is a

39 The pyruvate dehydrogenase multienzyme complex (Mr of 5-10 million) is assembled ar

40 es of the human 2-oxoglutarate dehydrogenase multienzyme complex (OGDHc), a rate-limiting enzyme in t

41 2) phosphorylates the pyruvate dehydrogenase multienzyme complex (PDC) and thereby controls the rate

42 The pyruvate dehydrogenase multienzyme complex (PDC) is a key regulatory point in c

43 s the activity of the pyruvate dehydrogenase multienzyme complex (PDC).

44 (E3) subunits of the pyruvate dehydrogenase multienzyme complex (PDH).

45 the Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc) and its E1 (ThDP-dependent) c

46 the Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc) by its coenzyme thiamin dipho

47 the Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc) has been determined at a reso

48 the Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc) has been determined with phos

49 the Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc), as a representative of the P

50 the Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc), binds to the enzyme with gre

51 The Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc), consisting of multiple copie

52 ferential regulation of the sulfur-oxidation multienzyme complex (SOX), which in S. denitrificans is

53 herichia coli's 2-oxoglutarate dehydrogenase multienzyme complex (termed BBL) with a combination of s

54 lation of biotin and is one component of the multienzyme complex acetyl-CoA carboxylase that catalyze

55 lation of biotin and is one component of the multienzyme complex acetyl-CoA carboxylase that catalyze

56 lation of biotin and is one component of the multienzyme complex acetyl-CoA carboxylase, which cataly

57 th variants displayed pyruvate dehydrogenase multienzyme complex activity at levels of 11% (Y177A E1)

58 us stearothermophilus pyruvate dehydrogenase multienzyme complex adopts a unique, compact structure.

59 ated the composition and organization of the multienzyme complex alpha-ketoglutarate dehydrogenase (a

60 ering the understanding of its function in a multienzyme complex and in the membrane-bound P64K prote

61 performs two functions: It is a respiratory multienzyme complex and it recognizes a mitochondrial ta

62 dmark structure was the first structure of a multienzyme complex and the first structure revealing an

63 t of Escherichia coli pyruvate dehydrogenase multienzyme complex are essential for several catalytic

64 ch probably have consequences in the overall multienzyme complex assembly.

65 utida and P. aeruginosa encode the inducible multienzyme complex branched-chain keto acid dehydrogena

66 for its growth and produces an extracellular multienzyme complex called the cellulosome, which is inv

67 he entire assembly and characterization of a multienzyme complex can be completed within 1-2 weeks.

68 The multienzyme complex catalyzes the reversible oxidation o

69 nformation regarding recognition within this multienzyme complex class with an alpha(2) E1 assembly.

70 the entire family of homodimeric (alpha2) E1 multienzyme complex components, and should serve as a mo

71 lar eukaryotes, one of these assemblies is a multienzyme complex composed of eight proteins that have

72 integral component of T4 dNTP synthetase, a multienzyme complex containing phage-coded enzymes, whic

73 The Escherichia coli pyruvate dehydrogenase multienzyme complex contains multiple copies of three en

74 s by E3 or E1, respectively, showed that the multienzyme complex does not behave as a simple competit

75 domains of E1p relative to heterotetrameric multienzyme complex E1 components operating on branched

76 gnment of the E. coli pyruvate dehydrogenase multienzyme complex E1 subunit and yeast transketolase c

77 two enzymes are found in dNTP synthetase, a multienzyme complex for deoxyribonucleotide biosynthesis

78 virus (mORV) core particle is an icosahedral multienzyme complex for viral mRNA synthesis and provide

79 cetyl-CoA decarbonylase/synthase (ACDS) is a multienzyme complex found in methanogens and certain oth

80 (E2) component of the pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus is

81 The pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus was

82 ranscarboxylase is a 1.2 million Dalton (Da) multienzyme complex from Propionibacterium shermanii tha

83 ins both en route to the lysosome and in the multienzyme complex has remained elusive.

84 the acetyl-CoA decarbonylase/synthase (ACDS) multienzyme complex in Archaea.

85 beta-Gal and neuraminidase 1 (NEU1) form a multienzyme complex in lysosomes along with the molecula

86 It is the first structure of any multienzyme complex in pyrimidine biosynthesis and is a

87 restingly, GSK3beta can be released from the multienzyme complex in response to PKA phosphorylation o

88 viding biophysical evidence for a diffusible multienzyme complex in the mitochondrial matrix.

89 quivalent domain in a pyruvate dehydrogenase multienzyme complex in which the domain remains of const

90 anched-chain keto acid dehydrogenase (BCKAD) multienzyme complex involved in branched-chain fatty aci

91 n ketoacid dehydrogenase (BCKD) complex is a multienzyme complex involved in the catabolism of branch

92 the Escherichia coli pyruvate dehydrogenase multienzyme complex is an outcome of redistribution of a

93 omponent of the 2-oxoglutarate dehydrogenase multienzyme complex is composed of 24 subunits arranged

94 This multienzyme complex is itself regulated through reversib

95 ne-depleted conditions, these enzymes form a multienzyme complex known as the purinosome.

96 l and functional organization of the largest multienzyme complex known.

97 polypeptide from the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus assem

98 omponent of the pyruvate dehydrogenase (PDH) multienzyme complex of Bacillus stearothermophilus has i

99 In the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus, the

100 tyltransferase in the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus.

101 n interactions in the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus.

102 rase component of the pyruvate dehydrogenase multienzyme complex of Escherichia coli is catalysed spe

103 de chain of the 2-oxoglutarate dehydrogenase multienzyme complex of Escherichia coli was over-express

104 Glu139 of the large alpha-subunit of the multienzyme complex of fatty acid oxidation from Escheri

105 His450 of the large alpha-subunit of the multienzyme complex of fatty acid oxidation from Escheri

106 f the branched-chain keto acid dehydrogenase multienzyme complex of Pseudomonas putida.

107 mpromised tRNAs is unexpectedly rescued by a multienzyme complex shaped by constructive neutral evolu

108 th subunits of the fatty acid beta-oxidation multienzyme complex that are normally present in the mat

109 rom Propionibacterium shermanii is a 1.2 MDa multienzyme complex that couples two carboxylation react

110 oduces the prototypical cellulosome, a large multienzyme complex that efficiently hydrolyzes plant ce

111 Tryptophan synthase is an alpha2beta2 multienzyme complex that exhibits coupling of the alpha-

112 al gene copies of the pyruvate dehydrogenase multienzyme complex that have evolved into a pyruvate de

113 Resection is catalyzed by the resectosome, a multienzyme complex that includes bloom syndrome helicas

114 Component A3a is a multienzyme complex that includes the mcrC gene product,

115 troviral protease is a key enzyme in a viral multienzyme complex that initiates an ordered sequence o

116 dy, we demonstrate that this pathway forms a multienzyme complex that is associated with the nuclear

117 etases examined can be isolated as part of a multienzyme complex that is more stable, and consequentl

118 SAHH associates with DAO as part of a larger multienzyme complex that may function in planta as a nic

119 cetyl-CoA decarbonylase/synthase (ACDS) is a multienzyme complex that plays a central role in energy

120 s still carried out, but in the context of a multienzyme complex that remains structurally intact dur

121 onucleoside triphosphate biosynthesis form a multienzyme complex that we call T4 deoxyribonucleoside

122 novo purine biosynthetic pathway may form a multienzyme complex to facilitate substrate flux through

123 esized and posttranslationally modified by a multienzyme complex to their biologically active forms.

124 ang et al.(1) uncovers the pyrimidinosome, a multienzyme complex where enzymes from different subcell

125 A (PPCA), a serine carboxypeptidase, forms a multienzyme complex with beta-galactosidase and neuramin

126 Lysosomal neuraminidase-1 (NEU1) forms a multienzyme complex with beta-galactosidase and protecti

127 The Escherichia coli degradosome is a multienzyme complex with four major protein components:

128 NAC assembles a multienzyme complex with MetAP1 and NatA early during tr

129 The study revealed that the multienzyme complex with the active sites directed towar

130 n-regulated chloroplast protein CP12 forms a multienzyme complex with the Calvin-Benson cycle enzymes

131 he nucleus during S-phase, where they form a multienzyme complex with thymidylate synthase (TYMS) and

132 the Escherichia coli pyruvate dehydrogenase multienzyme complex with Y177A and Y177F substitutions w

133 is an independent folding domain of a large multienzyme complex, 2-oxoglutarate dehydrogenase.

134 nucleotide kinase (PNK) Grc3 assemble into a multienzyme complex, herein designated RNase PNK, to orc

135 sembles its catalytic apparatus into a large multienzyme complex, the cellulosome.

136 The reaction is catalyzed by a 0.8 MDa multienzyme complex, the editosome.

137 Evidence has been presented for a metabolic multienzyme complex, the purinosome, that participates i

138 y visualize de novo purine biosynthesis by a multienzyme complex, the purinosome.

139 By linking the MAP3K, MAP2K and MAPK into a multienzyme complex, these MAPK-specific scaffold protei

140 mponents of the 2-oxoglutarate dehydrogenase multienzyme complex.

141 onents of the E. coli pyruvate dehydrogenase multienzyme complex.

142 omponent of the human pyruvate dehydrogenase multienzyme complex.

143 pyruvate by the pyruvate dehydrogenase (PDH) multienzyme complex.

144 ications are believed to be carried out by a multienzyme complex.

145 Glu462 increases the thermostability of the multienzyme complex.

146 estigate the role that beta-Gal plays in the multienzyme complex.

147 , which confer quaternary flexibility to the multienzyme complex.

148 mbly of the cellulosomal components into the multienzyme complex.

149 iffer considerably from those of the larger, multienzyme complexes (cellulosomes).

150 encoding three alpha-ketoacid dehydrogenase multienzyme complexes (KADHs) that have central metaboli

151 r ROS generation, compromised affinities for multienzyme complexes and eventually clinical symptoms.

152 s about the functional significance of these multienzyme complexes and whether they might play a more

153 The pyruvate dehydrogenase multienzyme complexes are among the largest multifunctio

154 and disassembly of such naturally occurring multienzyme complexes are controlled.

155 he pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes are specifically recognised by the

156 type activity levels for E3 and all affected multienzyme complexes but are phenotypically normal.

157 nd E2 enzymes of the 2-oxoacid dehydrogenase multienzyme complexes by a previous model.

158 RNA degradosomes are multienzyme complexes composed of ribonucleases, RNA hel

159 ipoyl cofactor, which is employed by several multienzyme complexes for the oxidative decarboxylation

160 from the family of 2-oxo acid dehydrogenase multienzyme complexes form large protein scaffolds, to w

161 In nature, the catalytic efficiency of multienzyme complexes highly depends on their spatial or

162 hesis seems to be spatially regulated by the multienzyme complexes in a cluster-size-dependent manner

163 bition method may be a powerful way to study multienzyme complexes in their physiological context.

164 ofactor required for the function of several multienzyme complexes involved in the oxidative decarbox

165 tion are still unknown, but the formation of multienzyme complexes is considered a feasible Golgi pro

166 rogenase complex (PDC) is one of the largest multienzyme complexes known and consists of a dodecahedr

167 Multienzyme complexes of fatty acid oxidation from Esche

168 shown that glycolytic enzymes (GEs) exist as multienzyme complexes on the inner surface of human eryt

169 ic enzymes (GEs) have been shown to exist in multienzyme complexes on the inner surface of the human

170 essential cofactor for several mitochondrial multienzyme complexes required for oxidative metabolism.

171 Cellulosomes are multienzyme complexes responsible for efficient degradat

172 acteria, where they are assembled into large multienzyme complexes termed cellulosomes.

173 component of the three functional classes of multienzyme complexes that catalyze the oxidative decarb

174 drogenase is a common component of mammalian multienzyme complexes that decarboxylate alpha-ketoacids

175 Proteasomes are multienzyme complexes that maintain protein homeostasis

176 tabolism have long been hypothesized to form multienzyme complexes that regulate glucose flux in livi

177 ies (ROS) generation and impaired binding to multienzyme complexes were also addressed according to t

178 tly involved in the hydratase catalysis, the multienzyme complexes with either an alpha/Asp69 --> Asn

179 oprotein in at least two major mitochondrial multienzyme complexes would be consistent with a role in

180 Through anchoring and formation of multienzyme complexes, specific, localized signal transd

181 quired for the function of several essential multienzyme complexes, such as pyruvate dehydrogenase (P

182 H are structurally and catalytically similar multienzyme complexes, suggesting a common mode of inhib

183 e assembly of one of Nature's most elaborate multienzyme complexes, the cellulosome, results from the

184 e assembly of one of nature's most elaborate multienzyme complexes, the cellulosome.

185 ributing to allosteric signal propagation in multienzyme complexes.

186 mes, but via one or more membrane-associated multienzyme complexes.

187 ns catalysed by the 2-oxo acid dehydrogenase multienzyme complexes.

188 utarate dehydrogenase, and glycine reductase multienzyme complexes.

189 utarate dehydrogenase, and glycine reductase multienzyme complexes.

190 tute for mtLPD2 and associate with all these multienzyme complexes.

191 (E3) components, of 2-oxo acid dehydrogenase multienzyme complexes.

192 rix, are interchangeable among the different multienzyme complexes.

193 integral component of the function of these multienzyme complexes.

194 that react with components of mitochondrial multienzyme complexes.

195 gluconeogenesis, supporting the formation of multienzyme complexes.

196 cal role in stabilizing and regulating these multienzyme complexes.

197 e additional flexibility in highly populated multienzyme complexes.

198 the quaternary structure of highly populated multienzyme complexes.

199 ote cell proliferation often proceed through multienzyme complexes.

200 dihydrolipoyl moieties of four mitochondrial multienzyme complexes: pyruvate dehydrogenase, alpha-ket

201 ence that cell wall synthesis is mediated by multienzyme complexes; however, our results suggest that

202 icrobes, cellulases are assembled into large multienzymes complexes, termed "cellulosomes," which all

203 lyketide synthase was grafted onto the first multienzyme component (DEBS1) of the erythromycin-produc

204 acilitated dynamics of communication between multienzyme components.

205 The design of multienzyme composition (MEC) was applied to yield a hyd

206 ing leftovers (broiler necks), by means of a multienzyme composition, containing four commercially av

207 functions as the structural scaffold for the multienzyme degradosome complex.

208 is work, we re-evaluate FASP and the related multienzyme digestion (MED) FASP method.

209 ral sample preparation techniques, including multienzyme digestion and glycopeptide enrichment, to in

210 dy, we used glycoproteomic analysis based on multienzyme digestion followed by LC tandem MS analysis

211 The sample was then analyzed via our multienzyme digestion procedure followed by nano liquid

212 By extending multienzyme digestion strategies that use sample filtrat

213 ed dissociation (CID), in combination with a multienzyme digestion strategy (Lys-C, trypsin, and Glu-

214 nduced dissociation (CID) workflow involving multienzyme digestion, fractionation, OpeRATOR/SialEXO d

215 plement CRNs either used DNA-only systems or multienzyme DNA circuits.

216 T4 genes that encode other components of the multienzyme DNA replicase.

217 the spectrum in between DNA-only systems and multienzyme DNA systems.

218 Here, we describe a multienzyme effector complex (termed SHREC) that mediate

219 pathway, these factors can greatly simplify multienzyme engineering.

220 organization of the bacterial and eukaryotic multienzyme fatty acid synthase systems offer the prospe

221 open reading frames are expressed, identify multienzyme function and point to 'orphan' function.

222 chaeal PCNA 'tool-belt' recruitment model of multienzyme function that can facilitate both high fidel

223 Here we report a multienzyme-functionalized magnetic microcarriers-assist

224 electron transfers that are prevalent within multienzyme governed reactions.

225 lar to many non-ribosomal peptide synthetase multienzymes, has a central role.

226 rane environments and in the assembly of the multienzyme hyperstructures of bacterial cell wall biosy

227 flecting how difficult it is to purify these multienzymes in amounts adequate for kinetic and structu

228 Two levels of multienzyme labeling were used to measure a broad concen

229 fication strategy of graphene sheets and the multienzyme labeling, the developed immunosensor showed

230 A multienzyme layer containing choline oxidase (ChOx) and

231 oteins with ubiquitin is mediated by dynamic multienzyme machinery (E1, E2, and E3).

232 A multienzyme metabolic assembly for human glucose metabol

233 our results reveal a functionally relevant, multienzyme metabolic complex for glucose metabolism in

234 aching and FRET corroborate the formation of multienzyme metabolic complexes in living cells, which a

235 glycolytic and gluconeogenic enzymes into a multienzyme metabolic condensate, the glucosome.

236 Bacterial RNA degradosomes are multienzyme molecular machines that act as hubs for post

237 Previously, the studies on multienzyme nanocomplexes assembled on DNA scaffolds dem

238 Human XPA is an essential component in the multienzyme nucleotide excision repair (NER) pathway.

239 tic map (EASyMap)-guided streamlined one-pot multienzyme (OPME) and stepwise OPME (StOPMe) glycosylat

240 readily obtained by highly efficient one-pot multienzyme (OPME) reactions.

241 uity, they were successfully used in one-pot multienzyme (OPME) sialylation systems for highly effici

242 ed by NmSiaD(W)-dependent sequential one-pot multienzyme (OPME) strategy with in situ generation of t

243 6-legionaminyltransferase AbGtr18 in one-pot multienzyme (OPME) synthesis with or without additional

244 e conversion of DBT to HBP is catalyzed by a multienzyme pathway consisting of two monooxygenases and

245 The multienzyme pathway for heme formation culminates with t

246 ed by directed evolution and introduced into multienzyme pathways may lead to improved whole-cell cat

247 Several multienzyme pathways, including the excision repair of d

248 ture that is shared with other key branched, multienzyme pathways, such as glycolysis, where pathway

249 used to control PG hydrolases present within multienzyme PG-remodelling machines.

250 Here we report a multienzyme photobiocatalytic cascade to stereoselective

251 Cellulosomes are large, multienzyme, plant cell wall-degrading protein complexes

252 Sumoylation occurs by a multienzyme process similar to ubiquitination and, in Sa

253 to 5-bromoskatole, obviating the need for a multienzyme process.

254 This method has the potential for fast multienzyme protein adduct screening with high efficienc

255 sion of glucose into fatty acids through the multienzyme protein fatty acid synthase (FASN), for brai

256 so initiate blood digestion as components of multienzyme proteolytic complexes in malaria, platyhelmi

257 olled alignment of active sites promotes the multienzyme reaction efficiency.

258 ontrolled at nanoscale can have an effect on multienzyme reaction.

259 kinetic characteristics for this sequential multienzyme reaction.

260 Primosomes are multienzyme replication machines that contribute both th

261 ascent DNA chains during synthesis by the T4 multienzyme replication system in vitro.

262 DEAD-box helicase RhlB is a component of the multienzyme RNA degradosome assembly, and its interactio

263 xoribonuclease and integral component of the multienzyme RNA degradosome complex.

264 alic acid precursors by an efficient one-pot multienzyme sialylation system containing Pasteurella mu

265 nthases, which creates a direct link between multienzyme structure and the chemical structure of the

266 amphipathic helix at the N terminus of each multienzyme subunit which may promote dimerisation into

267 lyketide synthase composed of eight separate multienzyme subunits housing a total of 12 extension mod

268 They consist of several multienzyme subunits that must interact with each other

269 cular assembly lines that consist of several multienzyme subunits that undergo dynamic self-assembly

270 The multienzyme system (creatininase, creatinase, sarcosine

271 The fatty acid synthase type II (FAS-II) multienzyme system builds the main chain of mycolic acid

272 Our reconstituted multienzyme system revealed considerable promiscuity for

273 Here, we report a multienzyme system that supports Z-genome synthesis.

274 ted only when considering this ensemble as a multienzyme system, the functional parameters of which a

275 ro-protease to its active form in a coupled, multienzyme system.

276 tructural and functional modularity of these multienzyme systems has raised the possibility that poly

277 bility, and selectivity compared to the same multienzyme systems immobilized to solvent cast Nafion a

278 protein) is an integral component of several multienzyme systems involved in the tricarboxylic acid (

279 parallel interplay of evolutionary events in multienzyme systems leading to functional group diversit

280 n liquid-liquid or solid-liquid extractions, multienzyme systems linked to lactate dehydrogenase and

281 feasibility of investigating the effects of multienzyme systems on the structure of final glycan pro

282 s for individual substrates are modular, and multienzyme systems that can catalyse programmable metab

283 II polyketide synthases (PKSs) are bacterial multienzyme systems that catalyze the biosynthesis of a

284 polyketide synthases (PKSs) are a family of multienzyme systems that catalyze the biosynthesis of po

285 Biofuel cell researchers have studied multienzyme systems, but they have not investigated the

286 Multienzyme systems, on the other hand, aimed at using n

287 tty acid synthase (FASN) enzyme is a dynamic multienzyme that belongs to the megasynthase family.

288 sferase (trans-AT) polyketide synthase (PKS) multienzyme that was hypothesized to assemble gladiolin.

289 xyerythronolide B synthase (DEBS), are giant multienzymes that biosynthesize a number of clinically i

290 Such multienzymes typically use malonyl and methylmalonyl-CoA

291 Next, we developed a multienzyme workflow suitable for analysis of the differ