ライフサイエンスコーパス: multilocus sequence typing

1 action, pulse-field gel electrophoresis, and multilocus sequence typing).

2 KPC-KP isolates underwent multilocus sequence typing.

3 C. trachomatis samples were typed using multilocus sequence typing.

4 s from the same counties were compared using multilocus sequence typing.

5 to 2011, we identified 85 sequence types by multilocus sequence typing.

6 rsity of the isolates was investigated using multilocus sequence typing.

7 dom (approximately 600,000 people) underwent multilocus sequence typing.

8 ers or skin lesions, and were examined using multilocus sequence typing.

9 staphylococcal chromosomal cassette mec and multilocus sequence typing.

10 cile infection (CDI) fecal samples underwent multilocus sequence typing.

11 nfirmed by a number of biochemical tests and multilocus sequence typing.

12 elineated into 12 sequence types (STs) using multilocus sequence typing.

13 resolution of the E. coli relationships than multilocus sequence typing.

14 causing human disease were characterized by multilocus sequence typing.

15 articular animal sources was evaluated using multilocus sequence typing.

16 genes, pulsed-field gel electrophoresis, and multilocus sequence typing.

17 ts of two healthy children were genotyped by multilocus sequence typing.

18 eus [MSSA]) were genotyped by spa typing and multilocus sequence typing.

19 study, were assigned a sequence type (ST) by multilocus sequence typing.

20 nd environmental specimens were genotyped by multilocus sequence typing.

21 ith a more sensitive method, such as PFGE or multilocus sequence typing.

22 and sequence types (STs) were determined by multilocus sequence typing.

23 19F), pulsed-field gel electrophoresis, and multilocus sequence typing.

24 ptococcus pneumoniae, and isolates underwent multilocus sequence typing.

25 Meningococcal CCs were assessed by multilocus sequence typing.

26 pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing.

27 iverse and can be classified by serotype and multilocus sequence typing.

28 did whole genome sequencing and core genome multilocus sequence typing (1546 loci) on serogroup W di

29 ypic characterization of 300 NmY isolates by multilocus sequence typing, 16S rRNA gene sequencing, an

30 icd allele 96 and gyrB allele 87, two of the multilocus sequence typing alleles that define ST648; an

31 Multilocus sequence typing analysis for a variety of Shi

32 Multilocus sequence typing analysis of 49 B. bronchisept

33 Pulsed-field gel electrophoresis and multilocus sequence typing analysis showed that 24 (39%)

34 restriction fragment length polymorphism and multilocus sequence typing analysis.

35 We applied multilocus sequence typing and a microarray for detectio

36 valuation of genotypic (gene comparisons and multilocus sequence typing and analysis), genomic (dDDH,

37 We performed multilocus sequence typing and antifungal susceptibility

38 Isolates were characterized by multilocus sequence typing and antigen sequence typing o

39 multilocus restriction fragment typing, and multilocus sequence typing and assayed for the PVL virul

40 pes (sequence types [ST]) were defined using multilocus sequence typing and assigned to a clonal comp

41 borrelial DNA in ticks were characterized by multilocus sequence typing and by sequencing five other

42 cluding 322 NTHI strains, have been typed by multilocus sequence typing and found to have 359 sequenc

43 BLEC by pulsed-field gel electrophoresis and multilocus sequence typing and identified their blaESBL

44 cell lymphoma, which was identified both by multilocus sequence typing and matrix-assisted laser des

45 Isolates underwent multilocus sequence typing and PCR sequencing of the wzi

46 tudied, by pulsed-field gel electrophoresis, multilocus sequence typing and penicillin-binding protei

47 latedness of the isolates was assessed using multilocus sequence typing and phylogenetic analyses.

48 sely related porA sequences was confirmed by multilocus sequence typing and pulsed-field gel electrop

49 Multilocus sequence typing and pulsed-field gel electrop

50 Whole-genome multilocus sequence typing and single nucleotide polymor

51 DNA uptake sequence signatures, metagenomic multilocus sequence typing and strain-specific marker ge

52 Multilocus sequence typing and whole-genome sequence com

53 Isolates were characterised by serotyping, multilocus sequence typing, and antimicrobial susceptibi

54 chain reaction, phylogeny was assigned using multilocus sequence typing, and clonal relatedness was e

55 ermined by pulsed-field gel electrophoresis, multilocus sequence typing, and cmp gene (encoding the m

56 g homologous PFGE clusters were subjected to multilocus sequence typing, and eBURST was used to delin

57 ineage of each GBS isolate was determined by multilocus sequence typing, and isolates were clustered

58 ase genes, pulsed-field gel electrophoresis, multilocus sequence typing, and plasmid analysis were pe

59 ination of pulsed-field gel electrophoresis, multilocus sequence typing, and SCCmec typing.

60 Pulsed-field gel electrophoresis, multilocus sequence typing, and spa typing clustered the

61 typing by pulsed-field gel electrophoresis, multilocus sequence typing, and WGS.

62 Using serotyping, multilocus sequence typing, and whole-genome sequencing

63 gel electrophoresis (PFGE), ribotyping, and multilocus sequence typing are commonly used for this pu

64 he developed assays and a recently developed multilocus sequence typing assay showed congruent typing

65 ge of I. scapularis-borne Lyme disease using multilocus sequence typing based on bacterial housekeepi

66 mining of this enormous data set to create a multilocus sequence typing-based scheme that can identif

67 We show that emm typing and multilocus sequence typing can be achieved rapidly and e

68 Whole-genome sequencing was used to perform multilocus sequence typing, capsular typing, and identif

69 ing Ridom SeqSphere software for core genome multilocus sequence typing (cgMLST) analysis of whole-ge

70 Core genome multilocus sequence typing (cgMLST) and high-quality sin

71 strategies were used to generate core genome multilocus sequence typing (cgMLST) data.

72 ere, we describe a refinement to core genome multilocus sequence typing (cgMLST) in which alleles at

73 we proposed the development of a core genome multilocus sequence typing (cgMLST) scheme for M. gallis

74 We describe the creation of a core genome multilocus sequence typing (cgMLST) scheme to provide a

75 Based on core genome multilocus sequence typing (cgMLST), isolates from linea

76 ive whole-genome sequencing with core genome multilocus sequencing typing (cgMLST) analysis for real-

77 e patients, belonged to the same core-genome multilocus sequence typing cluster with no more than 4 a

78 Serotype and multilocus sequence typing data for 426 pneumococci date

79 ce types and showed improved congruence with multilocus sequence typing data.

80 Comparison with the multilocus sequence typing database shows that strains o

81 We have reanalyzed the Campylobacter multilocus sequence typing database used in the previous

82 In summary, multilocus sequence typing demonstrated a high degree of

83 Multilocus sequence typing demonstrated that clones A an

84 ltiple strains expressed collagen adherence, multilocus sequence typing demonstrated that the majorit

85 Multilocus sequence typing demonstrates the spread of C.

86 ere characterized by susceptibility testing, multilocus sequence typing, DiversiLab, and plasmid anal

87 Multilocus sequence typing effectively distinguished 82

88 Relatedness was assessed by multilocus sequence typing, fitness was evaluated by gro

89 study to compare repetitive-sequence PCR and multilocus sequence typing for genotyping hospital- and

90 We compared Xpert C. difficile/Epi to multilocus sequence typing for identification of C. diff

91 Although the sequences of each of the seven multilocus sequence typing genes were identical in the t

92 brial adhesin gene: H subclone assignments), multilocus sequence typing, gyrA and parC sequence (fluo

93 ST17) were identified for the first time by multilocus sequence typing in an area where bathing had

94 Multilocus sequence typing indicates that C. difficile s

95 hole-genome sequencing was used to determine multilocus sequence typing information and identify gene

96 A multilocus sequence typing method was also established f

97 es and in three European countries presented multilocus sequence typing (MLST) alleles, sequence type

98 lecular characterization was performed using multilocus sequence typing (MLST) alongside traditional

99 Multilocus sequence typing (MLST) analysis was performed

100 bicans isolates from 44 different sources by multilocus sequence typing (MLST) and ABC typing of rRNA

101 ovine, porcine, ovine, and canine sources by multilocus sequence typing (MLST) and examined their pro

102 he specificity and sensitivity inferred from multilocus sequence typing (MLST) and genome-wide SNP-ba

103 ecular epidemiology of CR-KP infections with Multilocus sequence typing (MLST) and Matrix-assisted la

104 Multilocus sequence typing (MLST) and multi-virulence-lo

105 In this study, multilocus sequence typing (MLST) and multilocus variabl

106 he same patient were molecularly typed using multilocus sequence typing (MLST) and multispacer sequen

107 Isolates were characterized by multilocus sequence typing (MLST) and outer membrane pro

108 onserotypeable presumptive pneumococci using multilocus sequence typing (MLST) and partial sequencing

109 acterized a diverse collection of strains by multilocus sequence typing (MLST) and performed restrict

110 MRSA isolates were further genotyped using multilocus sequence typing (MLST) and pulsed-field gel e

111 minatory power of PHAT (98%) equaled that of multilocus sequence typing (MLST) and pulsed-field gel e

112 o high-resolution fingerprinting approaches: multilocus sequence typing (MLST) and pulsed-field gel e

113 virulence-associated genes were analyzed by multilocus sequence typing (MLST) and sequence analysis

114 We have used multilocus sequence typing (MLST) and serotyping to buil

115 Characterization of PVL-MSSA isolates by multilocus sequence typing (MLST) and spa typing in this

116 The data for these isolates included multilocus sequence typing (MLST) and staphylococcal pro

117 genotyping approaches: gyrB gene sequencing, multilocus sequence typing (MLST) and whole genome clust

118 ere tested for antimicrobial susceptibility, Multilocus Sequence Typing (MLST) and Whole Genome Seque

119 discriminatory power was compared to that of multilocus sequence typing (MLST) and whole-genome optic

120 terizes B. hampsonii using a newly developed multilocus sequence typing (MLST) approach and elucidate

121 In this study, we have used a multilocus sequence typing (MLST) approach employing the

122 sZ, gltA, groEL, ribC, and rpoB), applying a multilocus sequence typing (MLST) approach.

123 Serotyping and multilocus sequence typing (MLST) are primary methods to

124 pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) based on loci in actA,

125 etitive sequence-based PCR typing, and rapid multilocus sequence typing (MLST) by electrospray ioniza

126 and refrigeration, predominantly belonged to multilocus sequence typing (MLST) clonal complex (CC)-21

127 virulence genes, by which groups similar to multilocus sequence typing (MLST) clonal groups (CGs) co

128 d no strong correlation between CPS type and multilocus sequence typing (MLST) cluster, with the rema

129 Multilocus sequence typing (MLST) confirmed the strains

130 Although multilocus sequence typing (MLST) currently represents t

131 Multilocus sequence typing (MLST) data demonstrated that

132 nalysis of the population structure based on multilocus sequence typing (MLST) data derived from the

133 Data derived from the public multilocus sequence typing (MLST) database established a

134 A multilocus sequence typing (MLST) database for V. paraha

135 eles within the current Campylobacter jejuni multilocus sequence typing (MLST) database.

136 Multilocus sequence typing (MLST) demonstrated 21 known

137 pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) for 90 P. aeruginosa i

138 The introduction of multilocus sequence typing (MLST) for the precise charac

139 the association between Opa repertoires and multilocus sequence typing (MLST) genotypes.

140 Multilocus sequence typing (MLST) had a typeability of 1

141 Multilocus sequence typing (MLST) has become the most co

142 Multilocus sequence typing (MLST) has emerged as a power

143 Prior studies using multilocus sequence typing (MLST) have found specific GB

144 Comparison between WGS and multilocus sequence typing (MLST) identified major discr

145 compare the discriminatory power of PFGE to multilocus sequence typing (MLST) in typing Salmonella e

146 he 16S-23S rRNA intergenic spacer region and multilocus sequence typing (MLST) indicated a predominan

147 Multilocus sequence typing (MLST) is a genetic typing to

148 For this reason, multilocus sequence typing (MLST) is an appealing altern

149 Multilocus sequence typing (MLST) is the gold standard g

150 ation, selection, and recombination in seven multilocus sequence typing (MLST) loci from 94 invasive,

151 capsular biosynthetic loci, and 8 targeting multilocus sequence typing (MLST) loci were employed for

152 gold standard" typing method for Salmonella, multilocus sequence typing (MLST) may be more relevant t

153 ld standard" for bacterial molecular typing, multilocus sequence typing (MLST) may offer advantages.

154 e data obtained from the characterization by multilocus sequence typing (MLST) of 334 isolates of S.

155 We performed multilocus sequence typing (MLST) on 590 pneumococcal is

156 We performed multilocus sequence typing (MLST) on a limited sampling

157 We performed multilocus sequence typing (MLST) on all isolates and se

158 We formulated multilocus sequence typing (MLST) primers with six of th

159 A total of 43 known multilocus sequence typing (MLST) profiles (or single- o

160 Recent multilocus sequence typing (MLST) refined the relatednes

161 ted sequences of the three genes agreed with multilocus sequence typing (MLST) results for typing of

162 Multilocus sequence typing (MLST) revealed 24 sequence t

163 pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) revealed a high level

164 Multilocus sequence typing (MLST) revealed sequence type

165 Development of a multilocus sequence typing (MLST) scheme based on six ho

166 We, therefore, developed a multilocus sequence typing (MLST) scheme for B. burgdorf

167 A multilocus sequence typing (MLST) scheme for M. pneumoni

168 Copenhagen) was initially used to develop a multilocus sequence typing (MLST) scheme for Salmonella

169 In this work, we describe a multilocus sequence typing (MLST) scheme for V. parahaem

170 A multilocus sequence typing (MLST) scheme that uses the s

171 of the genetic diversity of this pathogen, a multilocus sequence typing (MLST) scheme was developed a

172 A single multilocus sequence typing (MLST) scheme was developed f

173 nnii isolates to compare the robustness of a multilocus sequence typing (MLST) scheme, based on conse

174 In this study, we introduce a multilocus sequence typing (MLST) scheme, comprised of s

175 Using a nine-gene multilocus sequence typing (MLST) scheme, we identified

176 e identified and used to develop an expanded multilocus sequence typing (MLST) scheme.

177 re of this genus, we developed a genus-level multilocus sequence typing (MLST) scheme.

178 Two multilocus sequence typing (MLST) schemes have been deve

179 d 167 isolates into 39 groups and subsequent multilocus sequence typing (MLST) separated a subset of

180 Similarly, no multilocus sequence typing (MLST) sequence type (ST) was

181 hypothesis by performing the first extensive multilocus sequence typing (MLST) survey of plumbing dra

182 clades are assigned to ST131 by the Achtman multilocus sequence typing (MLST) system and a screening

183 A multilocus sequence typing (MLST) system has been report

184 pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) to characterize them a

185 Here, we used multilocus sequence typing (MLST) to compare the molecul

186 from a London hospital and characterized by multilocus sequence typing (MLST) to determine the ident

187 Multilocus sequence typing (MLST) together with macrores

188 Multilocus sequence typing (MLST) was able to accurately

189 Nested multilocus sequence typing (MLST) was employed for case

190 Multilocus sequence typing (MLST) was performed on 107 B

191 Multilocus sequence typing (MLST) was proposed in 1998 a

192 repetitive-sequence-based PCR (rep-PCR) and multilocus sequence typing (MLST) were performed.

193 pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) were performed.

194 ncluded PCR detection of ply and psaA genes, multilocus sequence typing (MLST), 16S rRNA gene sequenc

195 Multilocus sequence typing (MLST), a sequence-based meth

196 ) were identified as sequence type (ST) 8 by multilocus sequence typing (MLST), all of which are char

197 isolates from Thailand, which were typed by multilocus sequence typing (MLST), and 44 isolates from

198 ing pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and clustered regular

199 notyped by pulsed field gel electrophoresis, multilocus sequence typing (MLST), and molecular capsule

200 riable number tandem repeat analysis (MLVA), multilocus sequence typing (MLST), and pertactin gene (p

201 ng, restriction endonuclease analysis (REA), multilocus sequence typing (MLST), and pulsed-field gel

202 ing pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and serotyping.

203 FGE), Staphylococcus protein A (spa) typing, multilocus sequence typing (MLST), and staphylococcal ca

204 esis, staphylococcal protein A (spa) typing, multilocus sequence typing (MLST), and staphylococcal ca

205 by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and staphylococcal pr

206 The isolates underwent multilocus sequence typing (MLST), as well as assays for

207 ed the clustering of esp-positive strains by multilocus sequence typing (MLST), but surprisingly, all

208 of sequence-based typing approaches, such as multilocus sequence typing (MLST), by substantially incr

209 has a resolving power comparable to that of multilocus sequence typing (MLST), is applicable to mixt

210 Sequence-based typing (SBT), analogous to multilocus sequence typing (MLST), is the current "gold

211 ed typing technique most recently evaluated, multilocus sequence typing (MLST), often lacks discrimin

212 ing pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), pbp1a PCR restriction

213 is (PFGE), repetitive element PCR (Rep-PCR), multilocus sequence typing (MLST), plasmid profiling, an

214 resis (PFGE) profiles were analyzed by using multilocus sequence typing (MLST), plasmid profiling, hy

215 -field gel electrophoresis (PFGE) and 7-gene multilocus sequence typing (MLST), provided limited reso

216 during 2002 to 2008 in Great Britain, using multilocus sequence typing (MLST), pulsed-field gel elec

217 lution antimicrobial susceptibility testing, multilocus sequence typing (MLST), spa typing, SCCmec ty

218 Using multilocus sequence typing (MLST), we have compared (i)

219 Multilocus sequence typing (MLST), which defines strains

220 2016 with the isolates confirmed as ST398 by multilocus sequence typing (MLST), which prompted retros

221 by using polymerase chain reaction (PCR) and multilocus sequence typing (MLST).

222 solates causing meningitis were genotyped by multilocus sequence typing (MLST).

223 Selected strains were evaluated by multilocus sequence typing (MLST).

224 Select isolates then underwent multilocus sequence typing (MLST).

225 pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST).

226 ribotyping, genome restriction mapping, and multilocus sequence typing (MLST).

227 signed to amplify the same genes analyzed in multilocus sequence typing (MLST).

228 ied fragment length polymorphisms (AFLP) and multilocus sequence typing (MLST).

229 pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).

230 erized using emm typing, sof sequencing, and multilocus sequence typing (MLST).

231 ewbould 305 (NCIMB 702892), were analyzed by multilocus sequence typing (MLST).

232 raditional method of amplicon sequencing for multilocus sequence typing (MLST).

233 ups within clonal groups defined by standard multilocus sequence typing (MLST).

234 sive) from 2011 to 2014 was characterized by multilocus sequence typing (MLST).

235 genomic clades of Bp, largely congruent with multilocus sequence typing (MLST).

236 onsistent with genomotypes assigned by using multilocus sequence typing (MLST).

237 Selected isolates were subjected to multilocus sequence typing (MLST).

238 in China, Sweden, and Egypt were subtyped by multilocus sequence typing (MLST).

239 y repetitive sequence-based PCR (repPCR) and multilocus sequence typing (MLST).

240 ism analysis (RFLP), PCR fingerprinting, and multilocus sequence typing (MLST).

241 owing: conventional, extended, and ribosomal multilocus sequence typing (MLST, eMLST, and rMLST); ant

242 for all CPSs and selected protein antigens, multilocus sequencing typing (MLST), and pulsed-field ge

243 ltiple different ancestral origins (based on multilocus sequence typing [MLST] analysis), and did not

244 rized into six sequence types (determined by multilocus sequence typing [MLST]) and 79 pulsed-field g

245 sequence-based methods for bacterial typing (multilocus sequence typing; MLST) allow rapid and global

246 burgdorferi diversification was confirmed by multilocus sequence typing of 18 clinical isolates at 18

247 Multilocus sequence typing of Borrelia hermsii isolates

248 population genetic exchange was confirmed by multilocus sequence typing of isolates and of uncultivat

249 Multilocus sequence typing of multiple isolates from the

250 omic groups identified among the isolates by multilocus sequence typing of the 16S rRNA, flaB, gyrB,

251 analysis of PCR amplified16S rRNA genes, and multilocus sequence typing of three housekeeping genes c

252 We performed susceptibility testing and multilocus sequence typing on 528 (95%) of 554 serotype

253 eptibilities were recorded, and we performed multilocus sequence typing on a sample of MRSA isolates.

254 s were serotyped; susceptibility testing and multilocus sequence typing on Salmonella enterica serova

255 as a strain discrimination tool than either multilocus sequence typing or 23S ribosomal gene typing

256 s of clinical M. abscessus isolates utilized multilocus sequence typing or pulsed-field gel electroph

257 6S-23S ribosomal RNA intergenic spacer type, multilocus sequence typing, or both.

258 Isolates underwent PCR-based phylotyping, multilocus sequence typing, PCR-based detection of 55 vi

259 Multilocus sequence typing performed on DNA extracted fr

260 re not easily differentiated on the basis of multilocus sequence typing, phylogenetic typing, or carr

261 histories distinct from those predicted by a multilocus sequence typing phylogeny based on partial se

262 iers, and blood cultures were analyzed using multilocus sequence typing, phylotyping, ESBL genes, pla

263 s pulsed-field gel electrophoresis (PFGE) or multilocus sequence typing profiles to that of known epi

264 from the patient's blood and analyzed using multilocus sequence typing, protein gel electrophoresis

265 ping by pulsed-field gel electrophoresis and multilocus sequence typing revealed that the PVL-positiv

266 Ribosomal multilocus sequence typing (rMLST) using ribosomal prote

267 ics of the strains, which were determined by multilocus sequence typing, sap typing, and the presence

268 We recently described a multilocus sequence typing scheme (MLST) for P. acnes ba

269 We have created the first multilocus sequence typing scheme (MLST) for P. larvae,

270 These were organized into a core genome multilocus sequence typing scheme (N gonorrhoeae cgMLST

271 in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i)

272 ity within the species based on the existing multilocus sequence typing scheme.

273 ation showed that the arcC genes used in the multilocus sequence typing schemes of these two species

274 perior accuracy and performance for standard multilocus sequence typing schemes, along with larger ge

275 smetic injections and the first report using multilocus sequence typing sequence data for determining

276 electrophoresis) patterns and the same MLST (multilocus sequence typing) sequence type (ST-1068) rega

277 Multilocus sequence typing showed both NVE and PVE isola

278 Multilocus sequence typing showed that most biotype V is

279 Also, use of multilocus sequence typing showed that some of the seque

280 Multilocus sequence typing showed various clades, with m

281 tion of several molecular typing techniques: multilocus sequence typing, spaA typing, pulsed-field ge

282 e development and evaluation of an effective multilocus sequence typing strategy for M. xenopi.

283 By use of multilocus sequence typing, Streptococcus pneumoniae iso

284 lectrophoresis (PFGE); however, conventional multilocus sequence typing (targeting 6 conserved loci)

285 Here we demonstrate by multilocus sequence typing that enterotoxigenic E. coli

286 Using multilocus sequence typing to assess relatedness at the

287 in the South Pacific - for Wolbachia, using multilocus sequence typing to characterize bacterial str

288 We have used serogrouping and multilocus sequence typing to characterize meningococci

289 In a prior study, we utilized multilocus sequence typing to describe A. lentulus as a

290 bility among replacing serotypes, we applied multilocus sequence typing to samples of 126 and 222 pne

291 Molecular techniques such as spa typing and multilocus sequence typing use DNA sequence data for dif

292 ing AscI and ApaI) and better than that with multilocus sequence typing (using six housekeeping genes

293 We used multilocus sequence typing utilizing eight chromosomally

294 Multilocus sequence typing was also performed.

295 Multilocus sequence typing was performed on a sampling o

296 Multilocus sequence typing was successfully completed on

297 In this study, multilocus sequence typing was used to investigate genot

298 Using multilocus sequence typing, we identified another strain

299 Using multilocus sequence typing, we investigated the genetic

300 are, and environmental data and whole-genome multilocus sequence typing (wgMLST) of clinical and envi