ライフサイエンスコーパス: mutagenesis

1 owever, limits our understanding of chemical mutagenesis.

2 aspects of protein function through directed mutagenesis.

3 d from electrophysiological measurements and mutagenesis.

4 A3B levels, and consequently limiting cancer mutagenesis.

5 leosomal DNA imposes a strand polarity on UV mutagenesis.

6 modifying the cellular genome by insertional mutagenesis.

7 diting tool that has revolutionized targeted mutagenesis.

8 tches of sequence, subsequently confirmed by mutagenesis.

9 acids, thus laying the grounds for rational mutagenesis.

10 identified by bioinformatics and insertional mutagenesis.

11 NOD mice using zinc-finger nuclease mediated mutagenesis.

12 ional filters, attention maps, and in silico mutagenesis.

13 p and is associated with a high frequency of mutagenesis.

14 on relationship of (Pl)EctA by site-directed mutagenesis.

15 i supports efficient multiplex site-directed mutagenesis.

16 ated differences in replication fidelity and mutagenesis.

17 ombination with functional assays and atomic mutagenesis.

18 rice protoplasts while minimizing untargeted mutagenesis.

19 r difference NMR (STD-NMR) and site-directed mutagenesis.

20 l instability, fragility, and repeat-induced mutagenesis.

21 nants were resolved further by site-directed mutagenesis.

22 rial expression and assay platform for rapid mutagenesis.

23 driver of NCC evolution, we used CRISPR-Cas9 mutagenesis(11) to disrupt edn, ednr and dlx genes in th

24 if of Kv1.5 through N-terminal truncation or mutagenesis abolished the mechanical stretch-mediated in

25 rolysis using a combination of site-directed mutagenesis, activity measurements using synthetic PNAG

26 pair is required to mitigate transcriptional mutagenesis after exposure.

27 his study demonstrates that structure-guided mutagenesis aids the identification of critical determin

28 Here, employing site-directed mutagenesis, allelic exchange, quantitative PCR analyses

29 diates and iterative rounds of site-directed mutagenesis allowed us to identify 4 of 413 residues fro

30 y, using purified proteins and site-directed mutagenesis, along with fluorescence polarization and lu

31 inetic, molecular docking, and site-directed mutagenesis analyses confirmed these compounds as active

32 Extensive CT deletions and mutagenesis analyses helped us zoom in on three residues

33 rthermore, conserved secondary structure and mutagenesis analyses revealed a VB12-riboswitch, cbiMCbl

34 Furthermore, structural and mutagenesis analyses suggest several disease-related mut

35 An alanine mutagenesis analysis reveals that two receptor subsites

36 Using site-directed mutagenesis and a specific in vitro transcription assay,

37 In combination with alanine scanning mutagenesis and activity measurements we unveil the mole

38 NA structure-forming sequences can stimulate mutagenesis and are enriched at mutation hotspots in hum

39 tion, whereas human-mediated changes through mutagenesis and artificial selection led to duplication

40 ance of the identified site was validated by mutagenesis and biochemical modification.

41 By mutagenesis and biochemical studies, we demonstrated tha

42 nti-IL-13 antibody BAK1 as a result of InDel mutagenesis and combination with known point mutations v

43 Results from site-directed mutagenesis and competitive ligand-binding analyses reve

44 Further analyses by deletion mutagenesis and construction of chimeric mutants between

45 and suggests that the results of this InDel mutagenesis and conventional exploration of point mutati

46 Structure-guided mutagenesis and docking studies demonstrate the interact

47 ic component CntA were investigated based on mutagenesis and electron paramagnetic resonance (EPR) sp

48 We also used mutagenesis and electrophysiology in Xenopus laevisoocyt

49 s were performed together with site-directed mutagenesis and enzyme kinetic studies to identify resid

50 Cysteine scanning mutagenesis and EPR spectroscopy identified Cys-261 on C

51 Here, we used X-ray structure-guided mutagenesis and functional studies to identify amino aci

52 vided by the DGAT1 structures, together with mutagenesis and functional studies, provide the basis fo

53 Combining structural data with mutagenesis and functional studies, we identify key resi

54 acids in the active site and tested these by mutagenesis and in planta assays of necrosis induction b

55 cture, and verified them using site-directed mutagenesis and in vitro hormone-production assays using

56 ied signatures highly specific to alkylation mutagenesis and indicate the pervasive nature of alkylat

57 nome editing tool platform for site-specific mutagenesis and integration of up to more than 3 kilobas

58 Through a combination of bioinformatic, mutagenesis and kinetic approaches we demonstrate that t

59 A combination of mutagenesis and kinetic studies corroborate such a role.

60 Computational docking, validated by mutagenesis and kinetic studies, indicates that AK-42 bi

61 tors for RSPO3 using a combination of ligand mutagenesis and ligand engineering.

62 Site-directed mutagenesis and mass spectrometry experiments showed tha

63 tiana tabacum L.) using CRISPR site-directed mutagenesis and overexpression assays.

64 ii (CrHydA1) we have conducted site-directed mutagenesis and protein film electrochemistry to determi

65 NLR) immune receptor gene was isolated using mutagenesis and R gene enrichment sequencing (MutRenSeq)

66 tion, we applied diverse iterative cycles of mutagenesis and selection, thereby raising k(cat)/K(m) 2

67 ntarity-determining region 3 (HCDR3)-focused mutagenesis and selection.

68 We used site-directed mutagenesis and single-cell patch-clamp to analyze the f

69 me shows cooperativity between subunits, and mutagenesis and structural data provide clues to the cat

70 Site-directed mutagenesis and structural modeling analyses directly im

71 for sensitively detecting and characterizing mutagenesis and the early clonal evolutionary hallmarks

72 We applied hypersaturated transposon mutagenesis and transposon-directed insertion-site seque

73 e the ER and to retain CD58 are separable by mutagenesis and, possibly, by evolution, since chimpanze

74 g cryo-electron microscopy, structure-guided mutagenesis, and cell-based assays, we reveal the bindin

75 help of isotopic labeling and site-directed mutagenesis, and complemented by integration and interpr

76 ugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes.

77 chemical modification of Trp9, site-directed mutagenesis, and crystallographic and computational chem

78 ling, coevolutionary analyses, site-directed mutagenesis, and electrophysiology to examine the role o

79 tein modeling, ligand docking, site-directed mutagenesis, and electrophysiology, we show that TETS is

80 strong interactions, performed site-directed mutagenesis, and expressed the mutants in CHO cells.

81 Sr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning.

82 e use a combination of biochemical analyses, mutagenesis, and molecular simulations to show that XOAT

83 Here we introduce a transposon-based mutagenesis approach (TRIAD) to generate libraries of ra

84 ase activity, we employed a structure-guided mutagenesis approach and identified residues that are im

85 Here we take a mutagenesis approach, making viruses with single or mult

86 pus, together with an in vitro site-directed mutagenesis approach, we identify loss of STAT3 O-GlcNAc

87 bilizing mutp53(R175H) using a site-directed mutagenesis approach.

88 he B. distachyon genome using the Tnt1-based mutagenesis approach.

89 While targeted mutagenesis approaches like CRISPR/Cas9 now permit gene-

90 Asymmetric repair and mutagenesis are likely caused by differential accessibil

91 urrent methods for in vivo quantification of mutagenesis are limited because they rely on transgenic

92 SARS-CoV-2 and diligent tracking of ongoing mutagenesis are of key importance to plan robust strateg

93 ating 245 HOT loci with a massively parallel mutagenesis assay, we localized regulatory activity to a

94 A to be a mutagen that caused DNA damage and mutagenesis at least partly through increased reactive o

95 Through site-directed mutagenesis, based on a modeled structure, mutant enzyme

96 Here, using mutagenesis-based kinetic and pulldown analyses, we show

97 In this study, using site-directed mutagenesis, biochemical and biophysical tools, LC-MS/MS

98 its features that differ from previous point mutagenesis campaigns: while the average activity of TRI

99 ion, we show that the efficiency of knock-in mutagenesis can be further increased by electroporation

100 However, the barriers to mutagenesis can be overwhelmed under two sets of circums

101 These data clarify how insertional mutagenesis can modulate cell proliferation in CART19 th

102 or performing computational alanine-scanning mutagenesis (CASM) to guide experiments.

103 for SCID X1 were associated with insertional mutagenesis causing leukemia, because the viral enhancer

104 We performed site-directed mutagenesis, cellular localization experiments, and mole

105 We review techniques such as mutagenesis, chemical modifications, and optogenetics th

106 Analysis of chimeric TRPA1 proteins and mutagenesis combined reveals two amino acid residues loc

107 X-ray crystallography and mutagenesis confirmed a structure with a precisely desig

108 Site-directed mutagenesis confirmed the essential role that these resi

109 ments demonstrated that miRNA binding and 3' mutagenesis contribute to replication through mutually e

110 a high FRET value, which by structure-guided mutagenesis corresponds to a closed conformation of the

111 Mutagenesis coupled with surface plasmon resonance demon

112 n benchmarks, blind challenges, compensatory mutagenesis, cross-RNA homologies and internal controls

113 We aggregate these data with subsaturation mutagenesis data and use it to develop, test and validat

114 es remain less well-studied, in part because mutagenesis data for protein-nucleic acids interaction a

115 We analyze high-throughput mutagenesis data obtained for an alternatively spliced e

116 rapid method to analyse multiple transposon mutagenesis data sets allowing this technology to be use

117 EPR spectroscopy and mutagenesis data support that RumMC2 is a member of the

118 ood, thus it is suggested-and supported with mutagenesis data-that the pH change in the cellular mili

119 he small-molecule modulators corroborated by mutagenesis data.

120 idues, Phe(201) in P. aeruginosa IGPS, is by mutagenesis demonstrated to be important for the higher

121 s using X-ray crystallography, site-directed mutagenesis, deuterium-exchange MS, isothermal titration

122 APOBEC3A is a cytidine deaminase driving mutagenesis, DNA replication stress and DNA damage in ca

123 ns to validation using data on site-directed mutagenesis' effect on capsid assembly.

124 Targeted mutagenesis efficiency was further improved by an averag

125 microinjection, and report an improvement in mutagenesis efficiency when delivering CRISPR via electr

126 Using a combination of site-directed mutagenesis, electrophysiology, and modeling to probe bo

127 ied SthK channels using stopped-flow assays, mutagenesis, enzymatic catalysis and inhibition assays r

128 bition of the deamination process. In silico mutagenesis examinations further underpin the molecular

129 together with the kinetic isotope effect and mutagenesis experiments further outlines a few flexible

130 Key charge swap mutagenesis experiments provided evidence for pairwise i

131 Mutagenesis experiments reveal that the dimer-decamer eq

132 istent with this model, binding kinetics and mutagenesis experiments revealed that in solution the J-

133 o the proposed genome packaging signal (PS), mutagenesis experiments show that PS is not required for

134 Mutagenesis experiments show that this extension is disp

135 Mutagenesis experiments showed that the second luminal l

136 Results from mutagenesis experiments suggested that another FPC prote

137 id types together with electrophysiology and mutagenesis experiments.

138 r genomes occur due to internal and external mutagenesis forces.

139 t mice by whole-exome sequencing, transposon mutagenesis forward genetic screening, and transcriptomi

140 g the fluorescence-reported allelic exchange mutagenesis (FRAEM) technique and complemented the mutan

141 Alanine mutagenesis has identified residues that disrupt the ste

142 Genetic analyses and systematic mutagenesis have revealed that synonymous, non-synonymou

143 pulation, produced by ethyl methanesulfonate mutagenesis, hulled grains from 17 lines with elevated a

144 n one approach, we used in silico saturation mutagenesis, i.e. the scanning of all possible single am

145 to participate in specificity subsites, and mutagenesis identified a mobile lysine residue as a key

146 Electron microscopy combined with scanning mutagenesis identified ab8 interactions with all three S

147 Meiob mutagenesis identifies serine 67 as a critical residue f

148 o A3 enzyme target site selection and how A3 mutagenesis impacts mutation rates.

149 ombined with advances in transposon-mediated mutagenesis in a method termed transposon insertion sequ

150 ity of using Tnt1 to achieve near-saturation mutagenesis in B. distachyon, which will aid in function

151 e the pervasive nature of alkylation-induced mutagenesis in cancers.

152 e of oxidative stress-induced DNA damage and mutagenesis in cellular senescence and immortalization,

153 The findings indicate the potential of oat mutagenesis in combination with a high precision biochem

154 More broadly, we conclude that CRISPR mutagenesis in corals should allow wide-ranging and rigo

155 ships for APOBEC3A-catalyzed deamination and mutagenesis in driving multiple human cancers.

156 aching consequences for our understanding of mutagenesis in human aging and disease, and suggest that

157 , a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fu

158 the contribution of DNA repair towards indel mutagenesis in human cells.

159 Using N-ethyl-N-nitrosourea mutagenesis in mice in combination with a screening prot

160 e critical for hematopoiesis and in limiting mutagenesis in somatic tissues.

161 ctive features of photosensitization-induced mutagenesis in the immortalized cells, which were presen

162 ctions related to mutations or site-directed mutagenesis in titin that alter the I-band stiffness.

163 gliomas by genome-wide piggyBac insertional mutagenesis in vivo identifies 281 known and novel EGFR-

164 fatty acid binding protein was generated by mutagenesis, in which the gap is locked by a disulfide b

165 Our results from tatM2NX mutagenesis indicate that specific residues within the t

166 Results from ECT2 mutagenesis indicated that the tandem BRCT domain of ECT

167 of novel insertions by insertional-scanning mutagenesis (InScaM).

168 can be used to perform in silico saturation mutagenesis, interpret eQTLs, make predictions for struc

169 mental protein engineering, alanine-scanning mutagenesis involves the replacement of selected residue

170 Insertional mutagenesis is a powerful tool for both forward and reve

171 lts suggest that, unlike oxoG, oxoA-mediated mutagenesis is greatly induced by minor groove interacti

172 nant system that defends against OG-mediated mutagenesis is MutY/MutM1, and this system is dedicated

173 We show that conditional CRISPR mutagenesis is robust across many target genes and can b

174 in a directed evolution, the site-saturation mutagenesis library of Bacillus sp. MN chitosanase consi

175 Combined with mutagenesis, ligand binding and signaling studies, key i

176 The efficient mutagenesis makes it possible to detect large numbers of

177 Compared with oxoG, the mutagenesis mechanism of oxoA, which preferentially indu

178 se results further affirm structural variant mutagenesis mechanisms in sporadic disease traits, empha

179 re analysis revealed age- and gender-related mutagenesis mechanisms, characterized by high prevalence

180 bind alpha2-AP, and used an alanine-scanning mutagenesis method to select residues having higher acti

181 lex mutagenesis, over existing site-directed mutagenesis methods such as recombineering (recombinatio

182 ing populations or are introduced by induced-mutagenesis methods.

183 Here, using site-directed mutagenesis, molecular modeling, and in cell-free and ce

184 By using deep mutagenesis, mutations in ACE2 that increase S binding a

185 Site-directed mutagenesis near either FAD produced altered yields of t

186 This model was tested using targeted mutagenesis of amino acid residues within or adjacent to

187 Mutagenesis of basic residues herein, followed by functi

188 Site-directed mutagenesis of both cofilin and actin revealed residues

189 ER) with functional assays and site-directed mutagenesis of conserved residues to illuminate principl

190 neer AAV capsid libraries through saturation mutagenesis of different antigenic footprints.

191 Mutagenesis of exon 2 in Knock-in (KI) mouse models of t

192 Here, by performing deep mutagenesis of highly-included exons and by analysing th

193 Systematic mutagenesis of His583 to Ala, Asp, Asn, Glu, Gln, Lys, P

194 Thus, we created Cas9-mediated Arrayed Mutagenesis of Individual Offspring (CAMIO) to achieve c

195 PcUP1 with bound ligands, and site-directed mutagenesis of key residues provide additional support f

196 Site-saturation mutagenesis of Lys79 showed that changing Lys79 to any o

197 ed lateral protein organization, and surface mutagenesis of P2 coupled with structure-function experi

198 function of CHT7, we performed site-directed mutagenesis of previously identified phosphorylated amin

199 Mutagenesis of Pro(81) abolished the fragmentation, and

200 Mutagenesis of putative DAZL-binding sites in these mRNA

201 Alanine-scanning mutagenesis of Qtip shows that its localization and inte

202 l structures, combined with structure-guided mutagenesis of RGMs and BMP ligands, binding studies, an

203 tic cells and reveal that Ada contributes to mutagenesis of S (p)-Me-PTEs in E. coli.

204 R conformation and receptor signaling, while mutagenesis of the calcium-binding site abolishes Gpr126

205 is validated experimentally by site-directed mutagenesis of the key CcmK2 residue Serine 39.

206 stingly, preventing this recruitment through mutagenesis of the PCNA/Pol30 polymerase clamp or deleti

207 CRISPR/Cas9-mediated mutagenesis of the S. stercoralis tax-4 gene abolishes i

208 Mutagenesis of the single canonical conserved 3' UTR miR

209 uncovering its modulation of transcriptional mutagenesis of these lesions in human cells.

210 tivity of G. lovleyi NrfA, and site-directed mutagenesis of this arginine reduced enzymatic activity

211 Mutagenesis of U-loop residues reveals its contribution

212 hy, in vitro enzyme assays and site-directed mutagenesis, of the bacillaene synthase dehydratase/deca

213 The advent of unnatural amino acid mutagenesis opens up a new toolbox for the study of prot

214 Past studies, based on site-directed mutagenesis or solvatochromism of the isolated chromopho

215 TRACE induces high rates of mutagenesis over multiple cell generations in genes unde

216 editing catalytic polypeptide-like (APOBEC) mutagenesis over time, including a subset of patients wi

217 s advantages, including non-biased multiplex mutagenesis, over existing site-directed mutagenesis met

218 and rat in situ preparations, site-directed mutagenesis, patch-clamp, nerve recordings and pharmacol

219 Together with mutagenesis, peptide-binding studies and RagAB peptidomi

220 recent paper has concluded that a "templated mutagenesis" process is a major contributor to somatic h

221 multiple cryo EM maps, further modelling and mutagenesis provide working models for the mechanism for

222 Error-prone PCR mutagenesis reinforced the importance of R225 substituti

223 Using mutagenesis reporters spanning a well-defined replicon,

224 Here, purification and extensive mutagenesis revealed protein-protein interfaces required

225 Electrophysiological recordings and mutagenesis revealed that the signature sequence modulat

226 Mutagenesis reveals the aerobactin siderophore as the in

227 A structure-based mutagenesis scan identified surface-exposed regions and

228 Using functional analysis of a mutagenesis scan, biochemistry, and modeling, we find th

229 From an open UV mutagenesis screen for genetic suppressors of prp5 defec

230 In a forward mutagenesis screen for suppressors of the hpat1/3 low se

231 na) CCS52A2-deficient plants in a suppressor mutagenesis screen to identify APC/C(CCS52A2) substrates

232 describe the combination of high throughput mutagenesis screening studies, biochemical characterizat

233 Mutagenesis screens paired with single-molecule fluoresc

234 n vitro activity measurements and functional mutagenesis show that CDK7 activation can occur independ

235 Site-directed mutagenesis showed that the relevant sites for this regu

236 g transformations were achieved by a focused mutagenesis strategy and application of a novel technolo

237 bility and can serve as a platform for novel mutagenesis studies as well as a point for comparison wi

238 We combined systematic mutagenesis studies at the luteinizing hormone receptor

239 Our mutagenesis studies confirm that the CPSF30-hFip1 comple

240 Mutagenesis studies confirmed that inhibition of EBOV vi

241 Mutagenesis studies demonstrate the critical role of con

242 tiple sites, including Trp(72) Site-specific mutagenesis studies have suggested, but have not conclus

243 biotin biosynthesis genes during transposon mutagenesis studies in mouse infection models(6-9).

244 Furthermore, cross-linking and mutagenesis studies indicated that this second binding s

245 Mutagenesis studies of the galabiose-binding domain of t

246 gen/deuterium exchange mass spectrometry and mutagenesis studies reveal that the engagement of the di

247 Mutagenesis studies reveal that the region D1 in p27 pla

248 ral analysis together with computational and mutagenesis studies reveal the molecular mechanisms of t

249 ucture of the ATPaseCW cassette of MORC4 and mutagenesis studies show that the DNA-binding site and t

250 ctroscopy measurements and the site-directed mutagenesis studies show that the red Fur protein binds

251 Mutagenesis studies showed that LFNG modification of O-f

252 Site-directed mutagenesis studies were consistent with bisubstrate bin

253 This interaction was confirmed by mutagenesis studies, making 7-phenyl-2-aminoquinolines t

254 In line with mutagenesis studies, these gating modalities resulted fr

255 d by molecular dynamics (MD) simulations and mutagenesis studies, these structures reveal conformatio

256 Through mutagenesis studies, we show that phosphosites within th

257 tivator, thorough kinetic investigation, and mutagenesis studies.

258 dicted ARB affinity, which aligns with early mutagenesis studies.

259 cleotides in the 5' stem-loop is revealed by mutagenesis studies.

260 This property enabled a transposon mutagenesis study and growth studies to confirm novel ge

261 Using site-directed mutagenesis, surface plasmon resonance, and crystallogra

262 y of Polepsilon have a much weaker impact on mutagenesis than analogous Poldelta defects.

263 he bacterial ortholog of Polkappa, to induce mutagenesis that enables stress tolerance or escape.

264 ter their transcriptional profiles to foster mutagenesis, thereby enhancing the stochastic developmen

265 We performed mutagenesis to compromise the Zn(2+)-binding site and ob

266 Using a luciferase reporter system, mutagenesis to disrupt and restore base pairing revealed

267 lso used combinatorial and single-nucleotide mutagenesis to evaluate the in vivo impact of mutations

268 As proof-of-principle, we used site-specific mutagenesis to examine the role of NEMO in mediating RAN

269 marker, we used genetic crossover and random mutagenesis to generate new generations of topographies.

270 In the current study, we use site-directed mutagenesis to identify K(100) and K(188) as major sites

271 tooligosaccharide and utilized site-directed mutagenesis to identify residues involved in substrate b

272 Here, we employ genome-wide transposon mutagenesis to identify the genes that support clpB func

273 n, we used structure-guided alanine-scanning mutagenesis to map the functional epitope and paratope r

274 thermore, we coupled the assay to saturation mutagenesis to pinpoint functional regions within an enh

275 Here, using CRISPR Cas9 genome-wide mutagenesis to screen for genetic determinants of 2-OG l

276 Using site-directed mutagenesis to test multiple potential sites of phosphor

277 We used conditional mutagenesis to test whether these errors are due to the

278 e model to probe the contribution of APOBEC3 mutagenesis to viral evolution.

279 Evidence of APOBEC mutagenesis was found in 22% of the microbiopsies.

280 Site-directed mutagenesis was used to create orthogonal MTs possessing

281 Site-directed mutagenesis was utilized to assess the role individual a

282 position-based random insertion and deletion mutagenesis) was used to generate large libraries with r

283 Using site-directed mutagenesis, we demonstrate that the M159I substitution

284 Using heterologous in vivo assays and mutagenesis, we demonstrated that the C-terminal condens

285 To gain insights into oxoA-mediated mutagenesis, we determined crystal structures of poleta

286 Using site-directed mutagenesis, we determined that glycosylation of Asn-471

287 gh in-depth sequence analysis and systematic mutagenesis, we determined the consensus HEPN motif in t

288 Using mutagenesis, we extrapolate the functional importance of

289 Using site-directed mutagenesis, we generated FOXM1 mutant proteins that loc

290 Using in silico docking and site-directed mutagenesis, we identified Asp(230) in the extracellular

291 Using site-directed mutagenesis, we identified serine-465 as the site confer

292 By means of site direct mutagenesis, we identified TP receptor R295(7.40), which

293 In addition, using site-directed mutagenesis, we identify those amino acids that are spec

294 Through extensive site-directed mutagenesis, we map phosphorylation to the Env7 C termin

295 r affinity, and using molecular modeling and mutagenesis, we mapped its heparin-binding site.

296 ular dynamics simulations, and site-directed mutagenesis, we show that 8-NBD-cGMP inhibits PfPKG not

297 CRISPR GUARD reduces off-target mutagenesis while retaining on-target editing efficienci

298 ethods, which combine genome-wide transposon mutagenesis with high-throughput sequencing to estimate

299 at improved phenotypes in Mecp2/Y mice after mutagenesis with N-ethyl-N-nitrosourea (ENU).

300 Structure-based mutagenesis yielded a variant with considerably higher,