ライフサイエンスコーパス: myristoylation

1 be modified by lysine fatty acylation (e.g. myristoylation).

2 atty acid myristate to protein substrates (N-myristoylation).

3 critical for the infectivity of VACV and for myristoylation.

4 us of Arf and was largely independent of Arf myristoylation.

5 mutants, with changes at A4 and A5, undergo myristoylation.

6 d by posttranslational modifications such as myristoylation.

7 of NPHP3 and found the interaction requires myristoylation.

8 K(+) channel (hSlo1) might undergo internal myristoylation.

9 ter mobility of cytosolic form is due to its myristoylation.

10 required for membrane binding, possibly via myristoylation.

11 the ciliary base was dependent on N-terminal myristoylation.

12 sibility that Src might also be regulated by myristoylation.

13 dimer in solution independent of Ca(2+) and myristoylation.

14 lso depend on anchoring of the N terminus by myristoylation.

15 erminus and the other that is deficient in N-myristoylation.

16 calized to the plasma membrane by N-terminal myristoylation.

17 It also required HMG-coenzyme A and myristoylation.

18 he N-terminal domain of Nef encompassing its myristoylation.

19 h cGKII also associating with the BB via its myristoylation.

20 endent manner, but this effect also requires myristoylation.

21 logical activity of NCS-1 is governed by its myristoylation.

22 ndoplasmic reticulum, via N-terminal glycine myristoylation.

23 e conditionally exposed after a failure of N-myristoylation.

24 ARL13b stability, which is not observed with myristoylation.

25 FA in promoting oocyte fate through protein myristoylation.

26 ved in all poxviruses: a site for N-terminal myristoylation, 14 cysteines, and a C-terminal transmemb

27 Myristoylation, a 14-carbon lipid, is shown to largely s

28 ncated VP4 capsid protein lacking N-terminal myristoylation, a carboxyl-terminal pX extension of VP1,

29 istoyltransferase-1 (NMT1) catalyzes protein myristoylation, a lipid modification that is elevated in

30 Loss of myristoylation abolished the tumorigenic potential of Sr

31 Block of myristoylation abolished these effects, leaving regulati

32 y shift assays of ligand binding and peptide-myristoylation activity in scintillation proximity assay

33 hymus represents an organ with a prominent N-myristoylation activity.

34 Myristoylation affects both the rate and intensity of re

35 l study of the molecular mechanisms by which myristoylation affects protein folding and function, whi

36 channels and regulate them like CaM, whereas myristoylation allows differential, Ca2+-independent reg

37 Myristoylation also has almost no effect on protein fold

38 tifs for both endoproteolytic cleavage and N-myristoylation, although the function of these post-tran

39 Two recent papers show that, rather than myristoylation, amino-terminal acetylation of the Arf-li

40 es with membranes via the combined action of myristoylation and a polybasic effector domain, which bi

41 cid (C14), a principal substrate for protein myristoylation and a potential peroxisomal beta-oxidatio

42 n this report, we investigated whether the N-myristoylation and Ca(2+)-binding domains of CHP3 are im

43 llular fractionation experiments showed that myristoylation and Ca2+ binding by Ncs1p are essential f

44 s reversible phase behavior is retained upon myristoylation and can be tuned to span a 30-60 degrees

45 SVIP is anchored to microsomal membrane via myristoylation and co-fractionated with gp78, Derlin1, p

46 lly overlapping Noonan syndrome, promoting N-myristoylation and constitutive targeting of the mutant

47 changes, and determine structural effects of myristoylation and dimerization.

48 dopsis and rice lack a transmembrane domain, myristoylation and GPI-anchor protein modifications.

49 We also demonstrate that myristoylation and membrane binding regulate c-Src ubiqu

50 t this property of GPC is independent of its myristoylation and of coexpression with the virus matrix

51 Myristoylation and palmitoylation are critical for paras

52 In contrast, HGAL myristoylation and palmitoylation avert its inhibitory e

53 that membrane targeting of v3 is mediated by myristoylation and palmitoylation of its N-terminal MGC

54 Myristoylation and palmitoylation of the N terminus of F

55 This was validated by direct myristoylation and palmitoylation studies, which indicat

56 Fatty acylation, such as myristoylation and palmitoylation, targets proteins to c

57 nuclear/Golgi region by virtue of N-terminal myristoylation and palmitoylation.

58 scopic methods, we analyzed the effects of N-myristoylation and phosphorylation at Ser10 on the inter

59 Point mutations abolishing either Neurl1 myristoylation and plasma membrane localization or Neurl

60 y abolished PM localization, suggesting that myristoylation and possibly the N-terminal domain contri

61 ry and that the protein does not contain the myristoylation and prenylation lipid-anchoring motifs th

62 t demonstration of a functional role for CNB myristoylation and reveal the importance of Nmt1 in modu

63 in caveolae and is dually acylated by both N-myristoylation and S-palmitoylation.

64 B13 exposure blocked Src myristoylation and Src localization to the cytoplasmic m

65 tituted for functional PKGI, suggesting that myristoylation and subsequent membrane association block

66 Together our results indicate that both the myristoylation and the CHCH domain are essential for the

67 , we systematically analyzed the role of the myristoylation and the CHCH domain in the import and mit

68 We concluded that myristoylation and the pocket residues regulate c-Src, b

69 he localization of BBLF1 at the TGN requires myristoylation and two acidic clusters, which interact w

70 This is the first report linking N-myristoylation and ubiquitin-proteasome-dependent proteo

71 spase-3-cleaved fragment (post-translational myristoylation) and that myristoylation of this fragment

72 g and activation, which are dependent on its myristoylation, and block JNK activation.

73 ational modifications including acetylation, myristoylation, and iron binding were identified using o

74 ading frame, yielding a potential site for N myristoylation, and that mutation of the critical glycin

75 Here, we show that CNB myristoylation antagonizes phosphatase activation in yea

76 effects of folding and function arising from myristoylation are profoundly different from the effects

77 and that the oncogenic effects conferred by myristoylation arise, in part, from the tendency of the

78 changes, and evaluate structural effects of myristoylation as studied by isothermal titration calori

79 rescent protein fusions, we demonstrate that myristoylation at glycine 2 and an acidic cluster (AC; a

80 a dual-specificity kinase in vitro and that myristoylation at its amino terminus promotes associatio

81 Myristoylation at the N terminus alters how calmodulin b

82 mbrane localization of TRAM is the result of myristoylation because mutation of a predicted myristoyl

83 McCPK1 undergoes myristoylation, but not palmitoylation in vitro.

84 rom mutation-function analysis reveal that Z myristoylation, but not the Z late (L) or RING domain, i

85 1 and 3 with alanines decreased hSlo1 direct myristoylation by 40-44%, whereas in combination decreas

86 Disruption of CNB myristoylation by mutation of the myristoylated glycine

87 by 40-44%, whereas in combination decreased myristoylation by nearly 90% and abolished the myristic

88 Our results indicate that although N-myristoylation causes no spectroscopically discernible c

89 sharing N-terminal sequence homology, lack a myristoylation consensus sequence.

90 t cannot be mimicked in vivo by heterologous myristoylation consensus sequences.

91 To determine whether myristoylation contributes to their distinctive modulato

92 On expression of myristoylation-defective (G2A) NKD2, neither NKD2 nor TG

93 i) kinase activation was not detected with a myristoylation-defective Nef (HIV-1(SF2)NefG2A) or with

94 When N-myristoylation-defective rapsyn-EGFP mutant (G2A) and RI

95 In Aspergillus nidulans, the N-myristoylation deficient swoF1 mutant was previously sho

96 -Darby canine kidney (MDCK) cells expressing myristoylation-deficient (G2A) Naked2.

97 A myristoylation-deficient mutant of the Fus1 protein abro

98 rinuclear immunostaining pattern, indicating myristoylation dependent association with nonmitochondri

99 nsing AMP-activated protein kinase, AMPK, is myristoylation dependent.

100 ntify an unexpected function for NKD2, i.e., myristoylation-dependent escort of TGF alpha to the baso

101 ds to a flagellar arginine kinase TbAK3 in a myristoylation-dependent manner and is responsible for i

102 o associate with membranes increases in an N-myristoylation-dependent manner, which is suggestive of

103 h the plasma membrane and Golgi complex in a myristoylation-dependent manner.

104 Differential, myristoylation-dependent regulation of presynaptic Ca2+

105 g energetics or NMR spectra, suggesting that myristoylation does not influence the structure of the m

106 By contrast, the myristoylation domain of MARCKS needed for membrane asso

107 G2A mutant Z proteins by the addition of the myristoylation domain of the tyrosine protein kinase Src

108 Global quantification of N-myristoylation during normal growth or apoptosis allowed

109 Unexpectedly, myristoylation efficiency of MA(NOS) in Escherichia coli

110 We also assessed the myristoylation efficiency of the mutants and their abili

111 Protein N-myristoylation enables localization to membranes and hel

112 Eukaryotes have conserved N-myristoylation enzymes, involving one or two N-myristoyl

113 show that in contrast to the case for c-Abl, myristoylation exerts a positive effect on c-Src kinase

114 sing a hippocalcin mutant lacking N-terminal myristoylation exhibit a small I(sAHP) that is similar t

115 In these pathways, N-myristoylation facilitates association of substrate prot

116 To elucidate the role of protein N-myristoylation for thymocyte development, we generated m

117 Mutation of residues essential for either N-myristoylation (G2A) or calcium binding (D123A) did not

118 Alanine substitutions of predicted myristoylation (glycine-2) and palmitoylation (cysteine-

119 Furthermore, we showed that the absence of myristoylation had an immunosuppressive effect on T cell

120 Myristoylation has been shown to be important for the me

121 the Ca(2+)-free and Ca(2+)-bound states, and myristoylation has no effect on protein structure and fo

122 Mutations that abolish SSP myristoylation have been shown to reduce pH-induced cell

123 xcept the first 34 amino acids important for myristoylation highly compromised the ability to transfo

124 n repertoire of co- and post-translational N-myristoylation in addition to validating tools for the p

125 provide important functional insight into N-myristoylation in plants by ascribing postembryonic func

126 tenuated, leading to the suppression of EGR2 myristoylation in plants.

127 These results support a role of N-myristoylation in the allosteric regulation of PKA-C.

128 se reverse genetics to study the role of SSP myristoylation in the context of the intact virion.

129 In order to examine the role of SSP myristoylation in the context of the intact virus, we us

130 chemical biology approach to explore protein myristoylation in the major human parasite P. falciparum

131 e pocket of the enzyme and inhibit protein N-myristoylation in trypanosomes.

132 e currently no methods for reversing protein myristoylation in vivo.

133 Instead, myristoylation-incompetent RA T cells hyperactivated the

134 esponse when compared to immunization with a myristoylation-incompetent version of the construct.

135 the protein to acidic membrane surfaces, and myristoylation increases the affinity by a factor of 10;

136 Myristoylation increases the barely detectable intrinsic

137 hermal titration calorimetric data show that myristoylation increases the degree of cooperativity; th

138 Among those, N-myristoylation increases the kinase affinity for membran

139 lar localization of Pto was independent of N-myristoylation, indicating that N-myristoylation is requ

140 These results suggest that myristoylation influences the protein conformation and C

141 Likewise, treatment with the myristoylation inhibitor 2-hydroxymyristic acid inhibite

142 ical proof of concept for the use of protein myristoylation inhibitors as a strategy to block prostat

143 Protein N-myristoylation is a 14-carbon fatty-acid modification th

144 N-myristoylation is a crucial irreversible eukaryotic lipi

145 Protein myristoylation is a means by which cells anchor proteins

146 N-myristoylation is a protein lipidation event in which my

147 N-myristoylation is a protein modification process in whic

148 Therefore, Pto myristoylation is a quantitative factor for effector rec

149 N-myristoylation is a ubiquitous class of protein lipidati

150 Protein N-myristoylation is a ubiquitous co- and post-translationa

151 N-myristoylation is an essential fatty acid modification t

152 Collectively, the data suggest that myristoylation is an important structural determinant of

153 Protein N-myristoylation is catalysed by N-myristoyltransferase (N

154 Although N-terminal myristoylation is catalyzed co-translationally within th

155 Myristoylation is critical for membrane association of S

156 antitative dose response for inhibition of N-myristoylation is determined for >70 substrates simultan

157 Therefore, protein myristoylation is indispensable in T cell development an

158 ranslocate to membranes, and the function of myristoylation is not established.

159 Our results show that myristoylation is required for Fus1-mediated tumor-suppr

160 myristoylated at its amino terminus and that myristoylation is required for membrane binding.

161 ndent of N-myristoylation, indicating that N-myristoylation is required for some function other than

162 phosphorylated form of pp28 is incorporated, myristoylation is required, and sequences within the fir

163 N-myristoylation is the covalent attachment of myristic ac

164 N-myristoylation is the irreversible attachment of a C(14)

165 e identity of the enzyme catalyzing internal myristoylation is unknown.

166 ivo, and show that selective inhibition of N-myristoylation leads to catastrophic and irreversible fa

167 These results suggest that SSP myristoylation may function late in the fusion process t

168 n by ULK1 was dependent on AMPK beta-subunit myristoylation, metabolic stress associated with elevate

169 mutant protein containing a consensus feline myristoylation motif (MA(NOS)) and examined the impact o

170 Two have putative targeting signals: a myristoylation motif and a nuclear localization sequence

171 Expression of this fragment, which lacks the myristoylation motif and unique domain, was sufficient t

172 A heterologous N-myristoylation motif complemented N-terminal deletion mu

173 The CIL-7 myristoylation motif is essential for CIL-7 function and

174 ed, the protein does not have the N-terminal myristoylation motif nor is it subject to proteolytic ma

175 The N-myristoylation motif of Pto complemented the cognate mot

176 An N-terminal myristoylation motif present in AKAP12alpha is shown to

177 Src protein-tyrosine kinase contains a myristoylation motif, a unique region, an Src homology (

178 The unique N-terminal end harbors a myristoylation motif, and we have shown here that PRMT8

179 uced HMGCLL1 sequence contains an N-terminal myristoylation motif; the putative modification site was

180 Expression of the Pto N-myristoylation mutant, pto(G2A), conferred recognition o

181 N-terminal fatty acylations (N-myristoylation [MYR] and S-palmitoylation [PAL]) are cru

182 Analysis of Ncs1p mutants lacking myristoylation (Ncs1p(G2A)) or deficient in Ca2+ binding

183 We found that neither the N-terminal myristoylation nor the cysteine-rich RING H2 domain of r

184 The most common type of myristoylation occurs at an N-terminal glycine.

185 Protein N-myristoylation occurs by a covalent attachment of a C14:

186 Myristoylation occurs mainly at hSlo1 intracellular loop

187 In this case, myristoylation occurs within a portion of the preprotein

188 5, alpha7, beta3, and beta4); (2) N-terminal myristoylation of a 19S subunit (Rpt2); and (3) phosphor

189 establish a role for the post-translational myristoylation of a caspase-3-cleaved fragment of HTT, h

190 e predicted acylated residues confirmed that myristoylation of a glycine residue in the 2nd position

191 tment of AMPK to the mitochondria requires N-myristoylation of AMPKbeta by the type-I N-myristoyltran

192 further supports efficient and unprecedented myristoylation of an N-terminal lysine side chain, provi

193 We investigated the subcellular location and myristoylation of AtCPK5, a member of the Arabidopsis CD

194 Myristoylation of BBLF1 both facilitates its membrane an

195 Moreover, myristoylation of EGR2 is required for its function in p

196 al PTMs: postproteolytic N-acetylation and N-myristoylation of filensin.

197 Myristoylation of G9 was unaffected by the near-N-termin

198 Myristoylation of GCAP1 does not significantly alter its

199 Myristoylation of HMGCLL1 affects its cellular localizat

200 PCLX-001 treatment impacts the global myristoylation of lymphoma cell proteins and inhibits ea

201 ther lentiviruses is dependent on N-terminal myristoylation of MA by host N-myristoyltransferase enzy

202 Similarly, both myristoylation of MARCKS and cleavage of MARCKS by calpa

203 The physiologic role of myristoylation of MsrA remains to be elucidated.

204 is myristoylated at its N terminus, and that myristoylation of Neurl1 targets it to the plasma membra

205 Here, we present the role of myristoylation of NSC-1 in governing Ca(2+) binding and

206 difference between PKG isoforms results from myristoylation of PKGII.

207 The myristoylation of PRMT8 results in its association with

208 is an essential protein, which catalyzes the myristoylation of protein substrates, often to mediate m

209 ticular use in providing evidence for native myristoylation of proteins of interest, proof of activit

210 N-Terminal myristoylation of proteins typically occurs cotranslatio

211 Myristoylation of PrP affected amyloid formation in two

212 Myristoylation of secreted eukaryotic proteins represent

213 he development of inhibitors that target the myristoylation of specific viral substrates more selecti

214 ases (NMT) catalyze co- or posttranslational myristoylation of Src family kinases and other oncogenic

215 In addition to abrogating myristoylation of Src family kinases, PCLX-001 also prom

216 genic potential in vivo We further show that myristoylation of Src kinase is essential to facilitate

217 While myristoylation of the amino terminus has long been known

218 Loss of expression or a defect of myristoylation of the Fus1 protein was observed in human

219 conserved to promote exposure and efficient myristoylation of the MA N terminus.

220 es alone or in combination does not abrogate myristoylation of the protein, suggesting utilization of

221 Myristoylation of the regulatory subunit of calcineurin

222 To test the hypothesis that myristoylation of the self-interacting GRASP domain rest

223 (post-translational myristoylation) and that myristoylation of this fragment is altered in a physiolo

224 observations provide clear evidence that the myristoylation of TRAM targets it to the plasma membrane

225 These findings indicate N-terminal myristoylation of Z plays a key role in arenavirus buddi

226 been presented on the relative importance of myristoylation, of ionic interactions between protein an

227 Myristoylation often primes for subsequent palmitoylatio

228 We identify myristoylation on a transmembrane protein, the microneme

229 molecular mechanism for the modest effect of myristoylation on binding, wherein the membrane provides

230 nce suggests additional regulatory roles for myristoylation on proteins.

231 Here, we investigated the effects of N-myristoylation on the structure, dynamics, and conformat

232 ybasic mutants by introduction of a site for myristoylation or by coexpression of betagamma failed to

233 Unlike prenylation and myristoylation, palmitoylation is a reversible covalent

234 ization at the plasma membrane via potential myristoylation/palmitoylation anchors.

235 e context of Src-S3C/S6C interferes with its myristoylation/palmitoylation.

236 suggests there may be an alternative type of myristoylation pathway in mammalian cells.

237 tests indicated that ACS-4-dependent protein myristoylation perceives and translates the FA level int

238 MIC7 and its myristoylation play a crucial role in the initial steps

239 Based on our results, we predict that myristoylation promotes binding of ChChd3 to the outer m

240 ce, we compared the structure, dynamics, and myristoylation properties of native FIV MA with a mutant

241 However, myristoylation rarely occurs at an internal amino acid r

242 A second, less common myristoylation reaction occurs internally at dibasic ami

243 most likely a transient intermediate of the myristoylation reaction of Nef and is modulated by agent

244 22), the predicted protein has an N-terminal myristoylation recognition sequence, and we show here th

245 Our data indicate that myristoylation recruits FSP1 to the plasma membrane wher

246 N-myristoylation refers to the attachment of myristic acid

247 Mutation at putative myristoylation residue glycine 2 altered plasma membrane

248 Thus, myristoylation restricts the membrane orientation of the

249 These modifications include N-myristoylation, S-acylation (palmitoylation), prenylatio

250 zation was reproduced using the heterologous myristoylation sequence from v-src.

251 estic and wild cats, encodes a non-consensus myristoylation sequence not utilized by its host or by o

252 pically driven to the plasma membrane by the myristoylation sequence of c-Src, and by mutation of a p

253 r findings indicate that residues of the FIV myristoylation sequence play roles in replication beyond

254 of spine fusion, an effect that required the myristoylation sequence.

255 chor to membranes through a 5' MGXXXS/T(R) N-myristoylation sequence.

256 modeling have suggested that the N-terminal myristoylation signal and the C-terminal FXXF motif in P

257 ies revealed that residues of the N-terminal myristoylation signal are fully exposed and mobile in th

258 Furthermore, the N-myristoylation signal is necessary but not sufficient to

259 C2 domain is functionally equivalent to the myristoylation signal of c-Src, suggesting that it is an

260 strate that substitutions of either the PKAc myristoylation signal or the FXXF motif only modestly re

261 Although the Gag-derived myristoylation signal targets the v-Abl protein to the p

262 zymes (NMTs), which recognize a six-residue "myristoylation signal" with consensus sequence: M(1)GXXX

263 d two other proteins that bear an N-terminal myristoylation signal, human immunodeficiency virus type

264 d their only GRASP65 relative (Grh1) lacks a myristoylation site and has even been suggested to act i

265 t, despite extensive sequence variation, the myristoylation site and SH2 binding motifs were complete

266 89 to 91 amino acids with an amino-terminal myristoylation site and six SH2 binding motifs, showing

267 Analysis of the golli protein identified a myristoylation site at the C terminus of the golli domai

268 MK44 truncated at a putative N-myristoylation site did not produce current when express

269 Mutation of the myristoylation site Gly-2 abrogated membrane location.

270 ristoylation because mutation of a predicted myristoylation site in TRAM (TRAM-G2A) brought about dis

271 Mutation of the myristoylation site on golli disrupted its association w

272 it did not evoke inhibition of NHE3 unless a myristoylation site was added.

273 A predicted N-terminal myristoylation site was functionally analyzed.

274 ow that the N-terminal domain, including its myristoylation site, and the second EF-hand, which is in

275 ene WRKY29 BSK5 variants mutated in the BSK5 myristoylation site, ATP-binding site, and kinase activa

276 ow that the N-terminal domain, including its myristoylation site, the central alpha-helix, and the C-

277 hat contains two automethylation sites and a myristoylation site.

278 d ligand-binding domains and three potential myristoylation sites located near the N terminus.

279 odds with (i) its lack of palmitoylation or myristoylation sites that tailor its isoforms AKAP18alph

280 gh consensus CK2, PKC phosphorylation, and N-myristoylation sites, and may represent an essential ste

281 , phosphokinase (PK)C phosphorylation, and N-myristoylation sites.

282 is abolished by a mutation that blocks its N-myristoylation, Snf4p is shifted to the nucleus.

283 , from 100% of control (AcylTyA-GFP) for the myristoylation tag and PIP(2)-binding domain, to one-thi

284 icle budding and into EMVs, including: (i) a myristoylation tag; (ii) a phosphatidylinositol-(4,5)-bi

285 ipid modifications such as palmitoylation or myristoylation target intracellular proteins to cell mem

286 vo and identify cis-targeting motifs such as myristoylation that are necessary for EV-cargo associati

287 Myristoylation, the covalent linkage of a hydrophobic C1

288 Myristoylation, the N-terminal modification of proteins

289 In the absence of myristoylation, the R state predominates, and consequent

290 co-/post-translational modification known as myristoylation, the transfer of myristic acid (a 14-carb

291 N-myristoylation was also indispensable for early and dist

292 d lpxM single and double mutants showed that myristoylation was altered at lower temperature.

293 AtCPK5 membrane association, indicating that myristoylation was essential for membrane binding.

294 Loss of the site of N-myristoylation was found to affect neither AKAP macrosco

295 ing was essential for both phenotypes, while myristoylation was less critical.

296 bstrate for plant N-myristoyltransferase and myristoylation was prevented by converting the glycine a

297 Myristoylation was studied as a means to introduce pepti

298 ur results suggest that targeting Src kinase myristoylation, which is required for Src kinase associa

299 Among these regulatory mechanisms is N-myristoylation, whose biological role has been elusive.

300 ng G i proteins to the membrane, the role of myristoylation with regard to interaction with activated