ライフサイエンスコーパス: nascent peptide

1 to monitor the sequence and structure of the nascent peptide.

2 of ribosome recognition of the features of a nascent peptide.

3 ific interactions between the tunnel and the nascent peptide.

4 TC, responding to the presence of a specific nascent peptide.

5 of introducing a charged amino acid into the nascent peptide.

6 o control the membrane insertion of the same nascent peptide.

7 slation when placed at the N-terminus of the nascent peptide.

8 bond formation and with the extension of the nascent peptide.

9 it that normalized FRET values of the mutant nascent peptide.

10 n ribosomal complexes that are devoid of the nascent peptide.

11 a helix by a single, distant mutation in the nascent peptide.

12 the non-coding gap region as well as of the nascent peptide.

13 nner, elongating rather than terminating the nascent peptide.

14 ected by the unique primary sequence of each nascent peptide.

15 ribosome exit tunnel and the sequence of the nascent peptide.

16 role in translation and secondary folding of nascent peptides.

17 e 60 (which eliminates part of the essential nascent peptide) also distorts these estimates.

18 understanding of the elements required for a nascent peptide and a small regulatory molecule to contr

19 regulated by the length and sequence of the nascent peptide and by the conformational state, detecte

20 some is able to monitor the structure of the nascent peptide and can stall in response to specific pe

21 doxical potential for interactions between a nascent peptide and eRF1 to obstruct the translation ter

22 Here, I discuss the nascent peptide and its tasks as it wends its way throug

23 tase, binds to ribosome after release of the nascent peptide and promotes dissociation of the class I

24 ested that specific interactions between the nascent peptide and the antibiotic in the ribosomal exit

25 lity establishes the spatial position of the nascent peptide and the oxyanion and places the amine ne

26 A(f) and tested for their incorporation into nascent peptides and full-length protein using an Escher

27 Tom1 interacts with aberrant nascent peptides and is essential to limit their accumul

28 ogether with the detection of intron-encoded nascent peptides and RPS6/RPL7-carrying complexes in the

29 Translation arrest directed by nascent peptides and small cofactors controls expression

30 onic interactions between positively charged nascent peptides and the negatively charged ribosome exi

31 RNA complex binds to the signal sequence of nascent peptides and then docks with FtsY at the membran

32 rovide evidence that ATI mRNA is anchored by nascent peptides and translated at the inclusion sites r

33 raction between the ribosome-bound drug, the nascent peptide, and the incoming amino acid, which coll

34 tem that is both large enough to accommodate nascent peptides, and that traverses the particle.

35 By analyzing nascent peptide- and antibiotic-dependent ribosome stall

36 10, whose mutation reduces the efficiency of nascent peptide- and antibiotic-dependent ribosome stall

37 tures of the amino acid sequence of the TnaC nascent peptide are recognized by the translating riboso

38 ppocampal neuronal cultures, over 50% of all nascent peptides are found in these stalled polysomes.

39 , which remove the initiator methionine from nascent peptides, are essential in all organisms.

40 odons and patches of charged residues in the nascent peptide as best-studied examples.

41 Thus, our study reveals a strategy whereby a nascent peptide assists the ribosome in detecting a smal

42 ch two or more mature proteins bind the same nascent peptide at distinct sites and a second "piggy-ba

43 by transient interactions formed between the nascent peptide binding groove with the P2/P3 peptide an

44 menon that requires CAT-tail addition to the nascent peptides by Rqc2.

45 Maintaining the fidelity of nascent peptide chain (NP) synthesis is essential for pr

46 ading frame on tmRNA that tags the defective nascent peptide chain for degradation.

47 ongation phase, amino acids are added to the nascent peptide chain in accordance with codon sequences

48 upling between the stalling of the elongated nascent peptide chain in the ribosome and its insertion

49 neither specific amino acids nor a specific nascent peptide chain length was required for AAP to inh

50 on efficiency by secondary structures of the nascent peptide chain, we performed a comparative analys

51 peptides containing a thioamide bond in the nascent peptide chain.

52 threonine in the penultimate position of the nascent peptide chain.

53 critically involves physical partitioning of nascent peptide chains into the lipid bilayer.

54 urs, specifically whether it can begin while nascent peptide chains of individual subunits are still

55 , is a conserved and primary modification of nascent peptide chains.

56 To probe the role of nascent peptide charges in ribosome function, we used a

57 The resulting nascent peptide code underlies the mRNA effects of hundr

58 the structure of the exit tunnel and/or the nascent peptide configuration in the tunnel.

59 basis for understanding how the drug and the nascent peptide cooperate to inhibit peptide bond format

60 me-mediated degradation of apoB targets both nascent peptides cotranslationally before translocation

61 sis that polyamines and the structure of the nascent peptide create a rate-limiting step in uORF tran

62 exit tunnel, the C-terminal sequence of the nascent peptide, critical for stalling, is in the immedi

63 ns, thereby triggering pathways for mRNA and nascent peptide degradation and ribosomal rescue.

64 Although ribosomal subunit dissociation and nascent peptide degradation are well-understood, the mol

65 An extreme manifestation of such response is nascent peptide-dependent ribosome stalling, involved in

66 ing of the molecular mechanisms of drug- and nascent peptide-dependent ribosome stalling.

67 A2503 mutations abolish nascent-peptide-dependent stalling at the leader cistron

68 odulate the ribosomal response to regulatory nascent peptides, determines the slow dissociation rate

69 Kinetic studies show that some nascent peptides dramatically inhibit rates of peptide r

70 ne disclose side-chain rearrangements of the nascent peptide during chain elongation.

71 e puromycin, which is then incorporated into nascent peptides during protein translation, thus leavin

72 translational folding pathway by keeping the nascent peptide dynamic until the full domain emerges.

73 nter of the large subunit and the site where nascent peptides emerge.

74 Some of these uORF-encoded nascent peptides enable responses to specific metabolite

75 g.Two of the bypassing signals, a cis-acting nascent peptide encoded by the first open reading frame

76 the second (a stretch of amino acids in the nascent peptide encoded by the first ORF) destabilizes p

77 In eukaryotes, a nascent peptide entering the endoplasmic reticulum (ER)

78 The nascent peptide exit tunnel and peptidyltransferase cent

79 inhibits protein synthesis by binding in the nascent peptide exit tunnel and trapping the release fac

80 ocation of the macrolide binding site in the nascent peptide exit tunnel at some distance from the pe

81 Macrolide antibiotics bind in the nascent peptide exit tunnel of the bacterial ribosome an

82 f newly synthesized polypeptides through the nascent peptide exit tunnel of the bacterial ribosome.

83 atic and bactericidal macrolides bind in the nascent peptide exit tunnel of the large ribosomal subun

84 It invades the nascent peptide exit tunnel of the postrelease ribosome

85 bits translation by binding in the ribosomal nascent peptide exit tunnel, trapping release factors RF

86 towards synergistic binders that occupy the nascent peptide exit tunnel.

87 daptation of the exit tunnel for hydrophobic nascent peptides, extensive remodeling of the central pr

88 ucture and function, and in mRNA binding and nascent peptide folding.

89 e activity to split ribosomes, targeting the nascent peptide for degradation through the ribosome qua

90 olide antibiotic in the tunnel positions the nascent peptide for interaction with the tunnel sensory

91 ATPase activity of Hsp70 chaperones to bind nascent peptides for proper folding, translocation or ma

92 factors (RFs) RF1 and RF2, which release the nascent peptide from the peptidyl tRNA after undergoing

93 alternative rescue factor B (ArfB) releases nascent peptides from ribosomes stalled on mRNAs truncat

94 dly inhibit the release of puromycin-labeled nascent peptides from ribosomes.

95 the clearance of ubiquitinated, tRNA-linked nascent peptides from ribosomes.

96 es are capable of cellular puromycylation of nascent peptides, generating emissive products without a

97 termediate states become inaccessible as the nascent peptide grows.

98 after the first transmembrane segment in the nascent peptide has emerged from the ribosome.

99 nce of an inducing antibiotic and a specific nascent peptide in the exit tunnel abrogate the ability

100 protein synthesis is most efficient when the nascent peptide in the ribosome carries an alanine resid

101 These data indicate that the TnaC nascent peptide in the ribosome exit tunnel interacts wi

102 osyl residue of the oligosaccharide chain of nascent peptides in the endoplasmic reticulum (ER).

103 Specific nascent peptides in the ribosome exit tunnel can elicit

104 es and outlines our current understanding of nascent peptide-induced ribosome stalling in regulating

105 Translation of the TnaC nascent peptide inhibits ribosomal activity in the prese

106 An ion channel protein begins life as a nascent peptide inside a ribosome, moves to the endoplas

107 A number of nascent peptides interact with the exit tunnel and stall

108 to ribosome forward movement, facilitated by nascent peptide interactions that disengage the ribosome

109 tional modification is to fine-tune ribosome-nascent peptide interactions.

110 nslational modifications which transform the nascent peptide into a stable and active enzyme.

111 Cotranslational insertion of the nascent peptide into the membrane brings the translating

112 The nascent peptide is attached to the A-site tRNA and not t

113 ization of SecM-arrested ribosomes shows the nascent peptide is covalently linked via glycine 165 to

114 During this time, the nascent peptide is expected to be elongated only 12 or 1

115 ion from transiently arrested to full-length nascent peptide is faster for peptides containing neutra

116 rates of initial peptide elongation steps as nascent peptide is formed.

117 roduce transient arrest (pausing) before the nascent peptide is fully elongated.

118 ester bond between the peptidyl tRNA and the nascent peptide is hydrolyzed.

119 In this mechanism, while the nascent peptide is still in the exit tunnel of the ribos

120 ed by both proteins synergistically, and the nascent peptide is transferred to the translocon.

121 bosomal exit tunnel, along with its resident nascent peptide, is no less susceptible to these consequ

122 study we show that opsin, within a range of nascent peptide lengths, targets and translocates equall

123 part of this conserved sequence acts at the nascent peptide level to stimulate the frameshifting, wi

124 ate in the termination process, the complete nascent peptide linked to the tRNA that decodes the fina

125 Nascent peptide-mediated anchoring of ribosome-mRNA tran

126 Nascent-peptide modulation of translation is a common re

127 el protein containing the C-terminal Asp-Pro nascent peptide motif (which interferes with translation

128 d at stop codons on full-length mRNA using a nascent peptide motif that interferes with translation t

129 n Escherichia coli, we identified additional nascent peptide motifs that stall ribosomes.

130 During this process, the nascent peptide navigates along 100A of tunnel from the

131 folding by reducing ribosome speed when the nascent peptide needs time to form and optimize the core

132 The results indicate that short nascent peptides of each of the three proteins are unrea

133 through the immunocapture of epitope tagged nascent peptides of reporter mRNAs.

134 t a shift-prone site is enhanced by specific nascent peptide or mRNA context features.

135 e inducible and underscore the importance of nascent peptide recognition by the ribosome for generati

136 The molecular mechanisms of the nascent-peptide recognition and ribosome stalling are un

137 mental reactions, peptide bond formation and nascent peptide release, during the elongation and termi

138 at the plasma membrane persisted only if the nascent peptide remained in complex with the translating

139 degraded by the cytoplasmic exosome, and the nascent peptides remaining in stalled 60S exit tunnels a

140 y structure can be acquired early, while the nascent peptide resides within the ribosomal exit tunnel

141 blematic sequence motifs are confined to the nascent peptide residues in the peptidyl transferase cen

142 degrade the aberrant mRNA and the incomplete nascent peptide, respectively.

143 Whereas Ltn1 polyubiquitylates these nascent peptides, Rqc2 directs the addition of C-termina

144 ct in concert with the previously identified nascent-peptide sensor, A2062, in the ribosome exit tunn

145 This transition depends on nascent peptide sequence at specific tunnel locations.

146 These results show that a nascent peptide sequence can influence translation conti

147 bosomal frameshifting due to features of the nascent peptide sequence that include the penultimate ph

148 l molecule is recognized along with specific nascent peptide sequences as a composite structure that

149 ture and to respond functionally to specific nascent peptide sequences is a fundamental property of t

150 Certain nascent peptide sequences, when within the ribosomal exi

151 ion kinetics depend on steric constraints on nascent peptide side chains and that confined movements

152 Evidence is presented suggesting that the nascent peptide signal may stimulate bypassing by destab

153 Here, we report single-molecule imaging of nascent peptides (SINAPS) to assess translation in live

154 Several nascent peptides stall ribosomes during their own transl

155 ngal arginine attenuator peptide (AAP), as a nascent peptide, stalls the translating ribosome in resp

156 We propose that ubiquitination of the nascent peptide starts on the 80S and continues on the 6

157 creases to 60% with a natural, heterologous, nascent peptide stimulator.

158 The ability to monitor the nascent peptide structure and to respond functionally to

159 efficient bypassing is contained within the nascent peptide synthesized prior to takeoff.

160 cis), or does it methylate this residue on a nascent peptide tethered to a T domain on another module

161 ation between the incoming glycyl-tRNA and a nascent peptide that otherwise is conducive to the drug

162 ality control (RQC) complex detects aberrant nascent peptides that remain stalled in 60S ribosomal pa

163 highlight several examples of conserved uORF nascent peptides that stall ribosomes to regulate gene e

164 system to elucidate the contribution of the nascent peptide, the drug, and the ribosome toward forma

165 The nascent peptides then pass through the exit tunnel befor

166 rt is a "folding vestibule" that permits the nascent peptide to compact and explore conformational sp

167 the presence of Trp12, are necessary for the nascent peptide to create a tryptophan binding/inhibitio

168 nse and respond to single side chains of the nascent peptide to induce local conformational changes.

169 e from the ribosome and translocation of the nascent peptide to tmRNA.

170 a showing cross-linking of the N terminus of nascent peptides to nucleotides of the 23 S ribosomal RN

171 en the RQC complex fails to deliver aberrant nascent peptides to the proteasome for degradation.

172 ay (MPRA) to measure mRNA translation, named Nascent Peptide Translating Ribosome Affinity Purificati

173 ide or in the proximity of the ribosome, the nascent peptide undergoes structural fluctuations on the

174 h this N-terminal coumarin of ribosome-bound nascent peptides was examined.

175 phobic probe, coumarin, at the N terminus of nascent peptides was investigated.

176 s modulated by the C-terminal segment of the nascent peptide, where the third-from-last residue plays

177 a Cdc48 hexamer is recruited to extract the nascent peptides, which are addressed to the proteasome

178 sing is the result of the interaction of the nascent peptide with components of the large ribosomal s

179 o this inhibition, as peptidyl-tRNA carrying nascent peptide with penultimate arginine or lysine resi

180 ns results from abnormal interactions of the nascent peptide with the erythromycin resistance methylt

181 itochondrial targeting sequence (MTS) on the nascent peptide with the mitochondrial import complex.

182 y condensation (C) domains, which couple the nascent peptide with the next programmed amino acid of t

183 clease, perhaps due to an interaction of the nascent peptide with the ribosome or the daaP mRNA.

184 arginine biosynthetic enzyme, functions as a nascent peptide within the ribosomal tunnel and negative

185 As a nascent peptide within the ribosome exit tunnel, it acts

186 The AAP acts as a nascent peptide within the ribosome tunnel to stall tran