ライフサイエンスコーパス: native chemical ligation

1 ced with cysteine to facilitate synthesis by native chemical ligation.

2 de novo formation of synthetic liposomes via native chemical ligation.

3 er-catalyzed azide-alkyne cycloaddition, and native chemical ligation.

4 as to site-specific protein modification and native chemical ligation.

5 atic approach to defined proteoglycans using native chemical ligation.

6 e beta-functionalized cysteines suitable for native chemical ligation.

7 ead-to-tail cyclization of peptides has been native chemical ligation.

8 cosylated forms of (Ser-)CCL1, by convergent native chemical ligation.

9 ncorporated into full-sized proteins through native chemical ligation.

10 rs them to a peptide-elongation site through native chemical ligation.

11 the synthesis of a novel subunit vaccine via native chemical ligation.

12 interfacing of sortase-mediated ligation and native chemical ligation.

13 -terminal cysteine-containing constructs via native chemical ligation.

14 was driven by genetically encoded orthogonal native chemical ligation.

15 strategically placed thiol groups, enabling native chemical ligation.

16 N-terminus of the IgG1 Fc glycoprotein using native chemical ligation.

17 our synthetic peptide segments by sequential native chemical ligation.

18 ombination of expressed protein ligation and native chemical ligation.

19 phase peptide synthesis in combination with native chemical ligation.

20 r synthetic zinc finger proteins produced by native chemical ligation.

21 thesis of proteins and of cyclic peptides by native chemical ligation.

22 EPO even without recourse to cysteine-based native chemical ligation, a concise synthesis of the par

23 fined orientation, as determined directly by native chemical ligation/affinity cleavage; by contrast,

24 nd a cyclic cell-penetrating peptide through native chemical ligation and a 2-cyanobenzothiazole moie

25 genetically directed incorporation of 7 with native chemical ligation and desulfurization to yield an

26 and highlights the potential application of native chemical ligation and expressed protein ligation

27 agnitude faster reaction in model studies of native chemical ligation and in the synthesis of a small

28 MPAA should find broad use in native chemical ligation and in the total synthesis of p

29 Native chemical ligation and intein-based methods allow

30 ptide synthesis and assembled using combined native chemical ligation and isonitrile-mediated activat

31 Aided by native chemical ligation and mirror image phage display,

32 Using native chemical ligation and orthogonal protection of se

33 sed in preparative peptide chemistry such as native chemical ligation and radical desulfurization as

34 alities in vivo were constructed by two-step native chemical ligation and separated using trapped IMS

35 gent synthetic strategy via a combination of native chemical ligation and Sortase A-mediated cyclizat

36 Three sequential cycles of native chemical ligation and subsequent thiyl radical re

37 Key reactions include native chemical ligation and the Staudinger ligation, co

38 The sequential combination of native chemical ligation and thiol-ene radical chemistry

39 s through a chemoenzymatic approach based on native chemical ligation and use them to determine sever

40 ein, we report total chemical synthesis, via native chemical ligation, and functional characterizatio

41 man MDM2 and MDMX chemically synthesized via native chemical ligation, and identified several peptide

42 ease of producing C-terminal thioesters for native chemical ligation applications.

43 The thioester-mediated native chemical ligation approach mimics, to some extent

44 Here we extend the utility of this native chemical ligation approach to non-cysteine contai

45 ogue, N33A, synthesized by a newly developed native chemical ligation approach.

46 strategies relying on coupling reagents and native chemical ligation are available, there is a conti

47 The feasibility of native chemical ligation at -Gln-Cys- enabled a converge

48 We have re-examined the utility of native chemical ligation at -Gln/Glu-Cys- [Glx-Cys] and

49 ys- is not appropriate for use as a site for native chemical ligation because of formation of signifi

50 sine at different regions of alpha-syn using native chemical ligation combined with a novel desulfuri

51 that is analogous to the role of cysteine in native chemical ligation, combining chemoselective thioe

52 Our method has customized native chemical ligation concept for glycoconjugates syn

53 ing its complete removal within 15 min under native chemical ligation conditions.

54 gments and examined their compatibility with native chemical ligation conditions.

55 catalyst 4-mercaptophenylacetic acid (MPAA), native chemical ligation could be performed at -Gln-Cys-

56 Solid-phase native chemical ligation/cyclization methodology appears

57 nner complementary to that with conventional native chemical ligation-desulfurization strategies.

58 rotein ligation, protein trans-splicing, and native chemical ligation-each have specific limitations.

59 tag at the C-terminus was straightforward by native chemical ligation followed by conversion of the C

60 Native chemical ligation followed by desulfurization is

61 ical synthesis of TFF3 and its homodimer via native chemical ligation followed by oxidative folding.

62 everal advancements for difficult sequences, native chemical ligation from smaller peptide fragments,

63 bled from four polypeptide segments by using native chemical ligation in a fully convergent fashion.

64 conditions; (viii). on-resin cyclization by native chemical ligation in an aqueous milieu; and (ix).

65 ynthesis of murepavadin using intramolecular native chemical ligation in combination with a concomita

66 de rapidly and quantitatively rearranges via native chemical ligation into an isopeptide-bonded head-

67 cyclic peptide bearing a thioester handle in native chemical ligation is shown by a high yielding lig

68 Application of native chemical ligation logic to the case of an N-termi

69 staples allowed us to use this motif in the native chemical ligation-mediated synthesis of a small p

70 rminal analogues of CCL-19 with the aid of a native chemical ligation method to investigate structure

71 lly (15)N-labeled analogue, Vpu(2-81), using native chemical ligation methodologies and also report a

72 Native chemical ligation (NCL) affords an N-linked chito

73 Although native chemical ligation (NCL) and related chemoselectiv

74 nd cyclization of a peptide probe exploiting native chemical ligation (NCL) and thiol-maleimide addit

75 Solid-phase peptide synthesis (SPPS) and native chemical ligation (NCL) are powerful methods for

76 Successful operation of the machine via native chemical ligation (NCL) demonstrates that even ch

77 Here we demonstrate that native chemical ligation (NCL) enables in situ synthesis

78 established a two-fragment route leveraging Native Chemical Ligation (NCL) followed by tandem desulf

79 y hydrophobic membrane-associated peptide by native chemical ligation (NCL) in an ionic liquid (IL) [

80 The broad utility of native chemical ligation (NCL) in protein synthesis has

81 Native chemical ligation (NCL) is widely applicable for

82 While native chemical ligation (NCL) is widely used, it is lim

83 r structures to proteins was performed using native chemical ligation (NCL) of a C-terminal peptide t

84 into a peptide sequence in order to perform native chemical ligation (NCL) of two peptide fragments.

85 enerate modular tension probes combining the native chemical ligation (NCL) reaction with solid phase

86 igated to segment Asp(1)-Asn(36) by means of native chemical ligation (NCL) to give the full sequence

87 Here we describe the use of native chemical ligation (NCL) to rapidly prepare phosph

88 exchanged by a chemoselective and reversible native chemical ligation (NCL) which can take place at N

89 with a cysteine-modified lysophospholipid by native chemical ligation (NCL), affording a noncanonical

90 is such as in peptide conjugation, extending native chemical ligation (NCL), and for regioselective d

91 utomated fast-flow peptide synthesis (AFPS), native chemical ligation (NCL), and high-throughput eval

92 ed through semisynthesis involving multipart native chemical ligation (NCL), but this can be very lab

93 In one approach, native chemical ligation (NCL), short, unprotected pepti

94 igh purity, we combined this technology with native chemical ligation (NCL).

95 g to a 38-residue leader peptide by means of native chemical ligation (NCL).

96 ration of a cysteine residue was achieved by native chemical ligation (NCL).

97 hat micelle-assisted reaction can facilitate native chemical ligations (NCLs) between a peptide-thioe

98 f two cysteine sites (C291 and C322) for the native chemical ligations (NCLs).

99 tilizing a semisynthetic scheme that employs native chemical ligation of a pentapeptide (HCDLP) to re

100 he beta-annulus-S-peptide was synthesized by native chemical ligation of beta-annulus-SBz peptide wit

101 ecifically labeled fluorescent analogs using native chemical ligation of synthetic peptides, followed

102 full-length alphaHL monomer was obtained by native chemical ligation of the central synthetic peptid

103 olecules containing cysteamine, analogous to native chemical ligation of thioesters with peptides wit

104 this problem, we synthesized crambin by the native chemical ligation of three segments (15 + 16 + 15

105 fficacy of chemical protein synthesis by the native chemical ligation of three segments and establish

106 synthesized the 46 amino acid polypeptide by native chemical ligation of two distinct sets of peptide

107 applicability of total protein synthesis by native chemical ligation of unprotected peptide segments

108 rotein was prepared by total synthesis using native chemical ligation of unprotected peptide segments

109 Native chemical ligation of unprotected peptide segments

110 This strategy, combined with native chemical ligation, provides convergent and rapid

111 The native chemical ligation reaction (NCL) involves reactin

112 The native chemical ligation reaction has been used extensiv

113 Without this tool, the powerful native chemical ligation reaction typically used to asse

114 d a chemically synthesized peptide using the native chemical ligation reaction.

115 urther to produce covalently linked PNTs via native chemical ligation, rendering ca. 100 nm-long nano

116 iques, namely solid phase peptide synthesis, native chemical ligation, Staudinger ligation, NCA polym

117 to rapidly analyze the compatibility of the native chemical ligation strategy for X-Cys ligation sit

118 We introduce a sequential native chemical ligation strategy that is suitable for t

119 and functional role of this mark, we used a native chemical ligation strategy to generate histone H4

120 l glycopeptide fragments involved a stepwise native chemical ligation strategy to provide the longest

121 re efficiently assembled via a semisynthetic native chemical ligation strategy.

122 ans-cyclooctene, oxime, reductive amination, native chemical ligation, Suzuki, Sonogashira, cross-met

123 ix coiled coil that is obtained by templated native chemical ligation (T-NCL) of PNA-peptide conjugat

124 Using the native chemical ligation technique, we synthesized the p

125 The introduction of native chemical ligation techniques to join unprotected

126 se peptide synthesis based on an adaption of native chemical ligation technology and recombinant DNA

127 A novel auxiliary-mediated native chemical ligation that enables peptide ligation t

128 (alpha)thioester; this segment is joined by native chemical ligation to a 66-aa Cys peptide, to yiel

129 To explore its role in catalysis, we used native chemical ligation to generate semi-synthetic SrtA

130 ino acid peptide via Lansbury aspartylation, native chemical ligation to join peptide 19 with the gly

131 We used native chemical ligation to synthesize the grafted cycli

132 Here we describe the application of native chemical ligation to the preparation of full-leng

133 (Pg-alpha-aminoacyl)cysteines 4a,b underwent native chemical ligations to form native dipeptides 3f,i

134 namely, diselenide-selenoester ligation and native chemical ligation, to assemble a 31.5 kDa phospho

135 n sequence, featuring up to 20-membered ring native chemical ligation transition states.

136 s of OMTKY3 with several serine proteinases, native chemical ligation was used for the total synthesi

137 Native chemical ligation was used to assemble 12 heptad

138 ombination of expressed protein ligation and native chemical ligation was used to attach these analog

139 Using solid-phase synthesis and native chemical ligation, we created a library of 30 Max

140 Using native chemical ligation, we have linked the Lys-D-Ala-D

141 Aided by mirror image phage display and native chemical ligation, we have previously discovered

142 Using native chemical ligation, we show that a mixture of pept

143 ospecific track, oxytocin was constructed by native chemical ligation, wherein the two building block

144 Native chemical ligation, which is widely used in the sy

145 ve strategic fragments to be assembled using native chemical ligation with a focus on maximal converg

146 Following native chemical ligation with a thioester-containing seg

147 ry-modified peptides can be directly used in native chemical ligations with peptide thioesters easily