ライフサイエンスコーパス: natively

1 Neither made IL-1ra natively.

2 This method natively accounts and corrects for background photons an

3 Natively, acid resistance in Escherichia coli is largely

4 ivity-dependent optogenetic manipulations of natively active ensembles, we further show that these sp

5 We observe that the catalytic site natively adopts a zwitterionic reactive form in which Cy

6 Misfolding of the natively alpha-helical prion protein into a beta-sheet r

7 ere, on the contrary, evidence for an almost natively alpha-helix-rich monomeric precursor state with

8 epithelial fusion failure 1 (EFF-1) protein natively anchored in cell-derived membrane vesicles.

9 In this study, we examined the coupling of natively and heterologously expressed nociceptin/orphani

10 reover, CD146(+) perivascular cells express, natively and in culture, molecular markers of the vascul

11 single-molecule real-time DNA sequencing to natively and nondestructively measure nucleosome positio

12 dence that the conformational composition of natively and nonnatively unfolded proteins fail to appro

13 experiment, and for structure prediction of natively and nonnatively unfolded states.

14 that the NTD is partially ordered when it is natively attached to the Tudor domain.

15 that contain natural anionic domains or that natively bind to anionic nucleic acids.

16 tly from cyanobacteria and establish that it natively binds canthaxanthin (CAN).

17 s of G protein coupled receptors (GPCRs) and natively binds parathyroid hormone (PTH) and parathyroid

18 ect pest Trichoplusia ni (TnCAD) that is not natively bound by wild-type Cry1Ac.

19 The complex contains up to three natively bound PQ molecules.

20 ts are large, protein-based organelles which natively carry out processes important for carbon fixati

21 n active GTPase and (ii) Cdc10-less hexamers natively co-exist with octamers.

22 ng that chaperones are able to sequester non-natively contacting residues.

23 le of the oxygenic ligand in Av MoFeP, which natively contains a Ser ligand (betaSer188) to the P-clu

24 A strain that natively contains this coumarate catabolism pathway, Aci

25 PS was pre-equilibrated in natively contaminated sediment.

26 n analysis of a villin-type headpiece domain natively devoid of F-actin binding activity, that of sup

27 uclear magnetic resonance (SSNMR) studies of natively diamagnetic uniformly (13)C,(15)N-enriched prot

28 ty patterns of 100-nt sequences derived from natively differential genomic regions, identifying E-box

29 To explore image compression, 40 natively digital fundus images were selected with good p

30 This is striking, because rat amylin is natively disordered and not previously known to form amy

31 ematic heat capacity analysis of a family of natively disordered complementary fragments of oxidized

32 d Msh6 NTR, revealing that the Msh6 NTR is a natively disordered domain that forms an extended tether

33 Diffusion of these natively disordered domains enables direct delivery of p

34 henylalanine-glycine' (FG)-nucleoporins, the natively disordered domains of which line the passageway

35 We have selected a partially solvated natively disordered fragment of Escherichia coli, thiore

36 Structural data from this natively disordered gammaC peptide enhances our understa

37 This natively disordered kinetic trap, interpreted as a molte

38 The interaction of light chain LC8 with the natively disordered N-terminal domain of IC induces heli

39 Modeling the structure of natively disordered peptides has proved difficult due to

40 with experiments, to model the structure of natively disordered polyglutamine peptides.

41 The natively disordered protein alpha-synuclein is the prima

42 prion protein family member, Shadoo (Sho), a natively disordered protein possessing structural featur

43 We conclude that 7B2 is a natively disordered protein whose function as an antiagg

44 Natively disordered proteins belong to a unique class of

45 Many globular and natively disordered proteins can convert into amyloid fi

46 A growing number of natively disordered proteins undergo a folding/binding p

47 ls two transient helical segments flanked by natively disordered regions and a third more stable heli

48 ures of PTM sites, domain context, predicted natively disordered regions and sequence alignments.

49 An automatic method for recognizing natively disordered regions from amino acid sequence is

50 rn recognition algorithm to the detection of natively disordered regions in proteins.

51 ylation and OGlcNAcylation commonly occur in natively disordered regions of proteins, and often have

52 nionizable residues in the growing family of natively disordered small proteins with full NMR assignm

53 alphaS, which exists in equilibrium with the natively disordered state at low [TFE] and with a highly

54 abetes, IAPP undergoes a transition from its natively disordered state to a highly ordered, all-beta-

55 have provided mechanistic insights into how natively disordered states, now commonly identified in b

56 nd activated (open) proteasomes degraded two natively disordered substrates at internal peptide bonds

57 iochemical analyses indicate that the NTR is natively disordered, and is only required for activation

58 ive region that is otherwise predicted to be natively disordered.

59 of these proteins, which are predicted to be natively disordered.

60 ts have unravelled many proteins that appear natively disordered.

61 Although long considered a natively endowed and fixed trait, working memory (WM) ab

62 he cell surface of genetically well-defined, natively Esp-deficient strains, and isogenic Esp-positiv

63 cattering in human and rat erythrocytes that natively express AQP1, in hemoglobin-free membrane vesic

64 ulated conditions in MDA-MB-231 cells, which natively express both phospholipase D1 and phospholipase

65 rin-coated vesicles in epithelial cells that natively express ENaC.

66 s are likely relevant in all cell types that natively express endogenous LRRK2, and provide insights

67 tern blot analysis of 3T3-L1 adipocytes that natively express FATP1 demonstrate a prominent 130-kDa s

68 34(+) cell-derived primary mast cells, which natively express MrgX2, responded to LL-37 for sustained

69 lock studies in rhesus monkeys, which do not natively express NFTs.

70 ptor activation in different cell lines that natively express NPY-receptors and proof the specificity

71 Using human BE(2)-C neuroblastoma cells that natively express opioid receptors and human embryonic ki

72 Human embryonic ventricular epicardial cells natively express PPARgamma, which explains the abundant

73 pression of InsP(3)R2 in RinM5F cells, which natively express predominately InsP(3)R3, increased the

74 ed human HL-60 promyeloleukemia cells, which natively express the P2Y14 receptor but had no effect in

75 g85A-specific lymphocytes as highly as Ag85A natively expressed by the bacterium.

76 re also able to observe interactions between natively expressed c-Myc and Bcr in leukemic cell lines.

77 n embryonic kidney cell line derivative, and natively expressed channel protein in a human colonic tu

78 Cofractionation of both natively expressed CNGA3 and CNGB1 (the modulatory subun

79 ence RNA (siRNA) was employed to silence the natively expressed Gbeta proteins in rat SG tissue and t

80 monstration of mGluR1/5 signal regulation by natively expressed Homer proteins has been elusive.

81 ologously expressed in non-neuronal cells or natively expressed in a murine neuroblastoma cell line.

82 ological activation of the ORAI channel trio natively expressed in all cells.

83 essed in HEK, HeLa, and melanoma A7 cells or natively expressed in LLC-PK1 and IMCD cells, is in the

84 s provide normative data for sodium channels natively expressed in mouse muscle and illustrate the ne

85 both Piezo1 and Piezo2 channel isoforms were natively expressed in rat dorsal root ganglia tissue.

86 ary (CHO) cells, as well as the Y2 receptors natively expressed in rat forebrain, are distributed in

87 or through Kv7.4/Kv7.5 heteromeric channels natively expressed in rat mesenteric artery smooth muscl

88 To determine which Galpha proteins may be natively expressed in SCG neurons, the presence of mRNA

89 es encoding a full-length enzyme that is not natively expressed in the target bacteria.

90 an that observed in a similar mutant that is natively expressed in Thermosynechoccocus but appears to

91 Thus, sustained cholinergic stimulation of natively expressed m2/m4 muscarinic receptors controlled

92 in nanodiscs, with cysteine cross-linking of natively expressed membrane-embedded MdfA variants.

93 15 with NPSLE) showed no antibodies against natively expressed N-methyl-D-aspartate receptors (NMDAR

94 Instead, natively expressed oleyl-acetyl-glycerol (OAG) (a struct

95 olecule is the most potent E-selectin ligand natively expressed on any human cell.

96 directly from tissue and highly enriched in natively expressed proteins exposed to the bloodstream.

97 The association can also be observed between natively expressed proteins in a panel of hematopoietic

98 co-localizes with Dbs in NIH 3T3 cells, and natively expressed Rac1 relocalizes in response to Dbs e

99 Isolated cerebellar neurons natively expressed RGS6, GABA(B)R, and GIRK channel subu

100 First, effects on a natively expressed signalling pathway, alpha(2)-adrenerg

101 asma mass spectrometry on heterologously and natively expressed TlpD, we determined that this domain

102 for mapping the genome-wide binding sites of natively expressed transcription factors.

103 with high-throughput immunoprecipitation of natively expressed transcription factors.

104 In conclusion, in glial cells, natively expressed TRPC3s mediates a novel form of Ca 2+

105 cleotide phosphate (NAADP) strongly activate natively expressed TRPM2 channels in Jurkat T cells.

106 FFA3, whether heterologously or natively expressed, coupled via PTX-sensitive G-proteins

107 This suggests that in organisms where GR is natively expressed, GR likely contributes to stress resp

108 is showed that Gbeta1, Gbeta2 and Gbeta4 are natively expressed.

109 we used a human mast cell-line, HMC-1, that natively expresses both receptors.

110 s NCC function in a mammalian cell line that natively expresses NCC, the mouse DCT (mDCT) cell line.

111 g sequenced on the same day as a tissue that natively expresses those genes.

112 onsistent with previous studies by others of natively expressing neurons.

113 ein-Barr virus (EBV)-transformed lymphocytes natively expressing the most common of these variants (G

114 old in human chondrocytes from an individual natively expressing the TRPV4(T89I) mutation.

115 both a T7 transcript of yeast tRNA(Phe) and natively extracted total bovine liver mt-tRNA activate P

116 Upon sample dissolution, Dox and natively fluorescent compounds were separated and detect

117 Natively fluorescent NAD(P)H are assayed directly, and n

118 l evidence for efficient CypA catalysis on a natively folded and biologically relevant protein substr

119 is system to denatured p38alpha derived from natively folded and inclusion body protein.

120 ing a conformationally diverse collection of natively folded and mutation-induced misfolded von Wille

121 ever, the sequence of reactions leading to a natively folded and oxidized protein remains unknown.

122 dination of the protein is carried out under natively folded conditions, the modification and ultimat

123 ee of frustration for a protein based on its natively folded configuration.

124 ing electrospray ionization (ESI) than their natively folded counterparts.

125 e prior in vitro results we obtained active, natively folded forms of the E. coli and Vibrio cholerae

126 ack the tightly packed tertiary structure of natively folded globular proteins.

127 In a natively folded protein of moderate or larger size, the

128 al an unprecedented mechanical behavior of a natively folded protein.

129 ges between different functional states of a natively folded protein.

130 ion, templating misfolded conformations in a natively folded protein.

131 Previous studies have shown that natively folded proteins are brittle under tension, unfo

132 sts with the mechanism of amyloidogenesis of natively folded proteins such as transthyretin and lysoz

133 These fibrils are assembled from initially natively folded proteins with an all antiparallel beta-s

134 Amyloid-forming IDPs, unlike natively folded proteins, have folding trajectories that

135 Unlike natively folded proteins, the molten globule state of ap

136 tate that is able to propagate by recruiting natively folded PrP.

137 15)N, (1)H]-TROSY correlation NMR spectra of natively folded reconstituted OmpX.

138 tion of this strategy to further stabilize a natively folded RNA conformation suggests an important e

139 We have exploited the growing database of natively folded RNA structures in the Protein Data Bank

140 Obtaining homogeneous population of natively folded RNAs is a crippling problem encountered

141 inimizing spurious interactions with stable, natively folded RNAs.

142 r nucleic acids) interact with and sequester natively folded soluble U1-70K into insoluble aggregates

143 cations for how natural proteins reach their natively folded states.

144 ceptor protein, PEB3, demonstrated that this natively folded substrate protein is not efficiently gly

145 together with its biologically relevant and natively folded substrate, the N-terminal domain of the

146 further supported by activation of PKR by a natively folded T7 transcript of tRNA(Phe)in vivo suppor

147 Non-natively folded variants of superoxide dismutase 1 (SOD1

148 l states that proteins may occupy, including natively folded, disordered and amyloid.

149 he genetic code expansion concept to produce natively folded, site-specific, and lysine-acetylated Si

150 t conditions, where the monomeric protein is natively folded.

151 nt secretion even though the product was not natively folded.

152 variety of symmetries, most of which are not natively formed by block copolymers, including squares,

153 ar toolkit to investigate protein assemblies natively formed in the context of human disease.

154 opportunity to study how signaling proteins natively gain distinct function.

155 Expression of natively glycosylated alpha dystroglycan and alpha sarco

156 Natively glycosylated and nonglycosylated recombinant fo

157 he binding affinity of the Cel7A CBM and the natively glycosylated Cel7A CBM-linker.

158 imulation to quantify cellulose binding of a natively glycosylated Family 1 CBM.

159 x vivo, both keratocan and lumican, in their natively glycosylated form, do form cross-links with col

160 The formation of a correctly folded and natively glycosylated HIV-1 viral spike is dependent on

161 tion profile, when the goal is to generate a natively glycosylated protein?

162 whereas non-cross-linked collagen I, IV, and natively glycosylated SLRPs are susceptible to degradati

163 They contain water soluble, natively highly fluorescent proteins, phycobiliproteins,

164 Fe-S covalency is much lower in natively hydrated Fd active sites than in HiPIPs but inc

165 namics of liquid water at the interface with natively hydroxylated (001) surfaces of InP and GaP phot

166 ATR dimer was found to form natively in bleached rod outer segments in vitro and whe

167 t regulation of TREK channels by PLD2 occurs natively in hippocampal neurons.

168 auto-processing of progenitor sgRNAs occurs natively in plants.

169 hage display for mapping vascular endothelia natively in tissue and for achieving vascular targeting

170 Eosinophils natively inhabit the small intestine, but a functional r

171 use of electromyographic (EMG) signals from natively innervated and surgically reinnervated residual

172 rst vertebrate Tc1 element described as both natively intact and functionally active, and given its r

173 Natively, IPP and DMAPP are generated by the mevalonate

174 For analytes natively lacking chemical signatures, refractive index-b

175 Natively larger vesicles are shown to rupture earlier an

176 of nitrite to aerobic digester operated at a natively low pH (<5.5).

177 lved, structural information on full-length, natively membrane-anchored fusogens is scarce.

178 In part, a lack of natively metastatic, chemoresistant in vivo models has l

179 AC cells BIC1, in which the HPP1 promoter is natively methylated, and subjected them to 5-aza-2'-deox

180 ted in physiological relevance because cells natively migrate within complex tissue microenvironments

181 ransport was disrupted, RNA was depleted, or natively misfolded CTFs were introduced into these cells

182 quiring large amounts of highly purified and natively modified histones to be analyzed by various tec

183 We tested for such interactions in the natively monomeric human eye lens protein gammad-crystal

184 incorporating heavier atoms than those found natively; most commonly, multi- or single-wavelength ano

185 structure of a stable G-hairpin formed by a natively occurring DNA sequence.

186 The isolation and extraction of natively occurring signaling peptides (SPs) from tissue

187 ntR is freely available and can be installed natively or by using a provided pre-configured Virtual M

188 tic processes and could be identified either natively or following intensification of EGFP using immu

189 scription, we employ realistic models of the natively oxidized surfaces which go beyond the commonly

190 We captured more than 2.9 million natively paired TCRalphabeta clonotypes from six healthy

191 allel microfluidics to generate libraries of natively paired, full-length TCRalphabeta clones, from m

192 sed DART-seq to simultaneously determine the natively paired, variable region heavy and light chain a

193 A FRET-sensor assay using natively phosphorylated IRSp53 reveals opposite conforma

194 Although movement between natively populated alternative conformations can be crit

195 ent RNA longer than that associated with the natively positioned TEC.

196 Gluconacetobacter diazotrophicus (Gd), which natively possesses an Ala residue in the position of the

197 Salmonella enterica natively possesses both the Pdu and Eut operons, and our

198 ow, using an in-gel fluorescence assay, that natively prepared 160 and 175 kDa minimal group II intro

199 n of the two isoforms of CX3CL1, as both are natively present in the brain.

200 ) addition, to stimulate the metabolism of a natively present microbial community.

201 indole, even though S. typhimurium does not natively produce indole.

202 They can be natively produced in E. coli in very large amounts, and

203 Using natively purified proteins, we demonstrate that the pyru

204 eveloped a JavaScript library, Cram-JS, that natively reads and decompresses the CRAM format on-the-f

205 s convert stable misfolded polypeptides into natively refolded proteins.

206 disruption of the MAL gene cluster, which is natively silent in laboratory fermentation experiments a

207 s larger than approximately 100 kDa, whereas natively smaller proteins are equidistributed.

208 alternative models include beta-helices and natively structured proteins.

209 ylated in vivo, and identification tools for natively SUMOylated proteins are rare.

210 They natively support the detection of arbitrary complex, nov

211 Here we show that natively synthesized and secreted BslA forms surface lay

212 uch a 2DEG--the SrTiO3(110)-(4 x 1) surface, natively terminated with one monolayer of tetrahedrally

213 sed by aggregation of transthyretin (TTR), a natively tetrameric protein involved in the transport of

214 ins are structurally different, varying from natively unfolded (alpha-synuclein and core histones) to

215 equilibrium between a cytosolic form that is natively unfolded and monomeric and a membrane-bound for

216 eveal that the TEAD-binding domain of YAP is natively unfolded and that TEAD binding causes localized

217 We show that both aS are natively unfolded at low temperature with different prop

218 phenylalanine-glycine motifs in filamentous, natively unfolded domains (FG domains) line the diffusio

219 has been previously proposed to exist in the natively unfolded form .

220 lpha-synuclein that ranges from a monomeric, natively unfolded form in cytosol to a physiologically f

221 a SNARE-complex chaperone over a monomeric, natively unfolded form to an amyloid-like aggregate that

222 previously unanticipated structural role for natively unfolded GLFG repeats as Velcro to link NPC sub

223 pressed primarily in peripheral neurons) are natively unfolded in monomeric form, but structured in p

224 Natively unfolded in solution, alphaS accumulates as amy

225 Alpha-synuclein is natively unfolded in solution, but can be induced to for

226 larly problematic for polypeptides which are natively unfolded in their unaggregated state, and perha

227 A pathway from the natively unfolded microtubule-associated protein Tau to

228 o in several aggregation states, including a natively unfolded monomer, a beta-sheet rich oligomer, o

229 alphaS was long thought to occur as a natively unfolded monomer, but recent work suggests it a

230 alphaS was long believed to be a natively unfolded monomer, but recent work suggests it a

231 as metastable multimers and not solely as a natively unfolded monomer.

232 Gauging the interactions of a natively unfolded Parkinson disease-related protein, alp

233 mplex (NPC) requires nucleoporins containing natively unfolded phenylalanine-glycine (FG) domains.

234 ucleocytoplasmic transport is an assembly of natively unfolded polypeptides, which contain phenylalan

235 iction of the conformational changes in this natively unfolded protein and to the stabilization of so

236 rn can bind to and catalyze the folding of a natively unfolded protein much more readily or effective

237 hydropathy correlation for the prediction of natively unfolded protein sequences provides perhaps the

238 demonstrate in this issue of Neuron that the natively unfolded protein tau can propagate indefinitely

239 Tau is a natively unfolded protein that binds to microtubules and

240 Human Tau (hTau) is a highly soluble and natively unfolded protein that binds to microtubules wit

241 AlphaSyn is a natively unfolded protein with unknown function and unsp

242 A natively unfolded protein, alpha-synuclein can adopt dif

243 Alpha-synuclein, a natively unfolded protein, can adopt a number of differe

244 esent observations indicating that ActA is a natively unfolded protein, largely random coil that is r

245 As expected for a natively unfolded protein, Trp 39 was highly solvent-exp

246 n spectra of alpha-synuclein, a prototypical natively unfolded protein, were obtained in the presence

247 found that YopE had the characteristics of a natively unfolded protein, with its N-terminal 100 resid

248 ystem in the cell's effort to deal with this natively unfolded protein.

249 nt with the notion that alpha-synuclein is a natively unfolded protein.

250 ding diseases (Parkinson's and Alzheimer's); natively unfolded proteins (as many as 30% of eukaryotic

251 cation of Raman spectroscopy to characterize natively unfolded proteins has been underdeveloped, even

252 Although many such natively unfolded proteins have been structurally analyz

253 We demonstrate that bovine core histones are natively unfolded proteins in solutions with low ionic s

254 Natively unfolded proteins present a challenge for struc

255 nclusion that FG Nups constitute a family of natively unfolded proteins supports the hypothesis that

256 in and its homologue beta-synuclein are both natively unfolded proteins that colocalize in presynapti

257 Natively unfolded proteins, mutations that unfold protei

258 n a mapping of the free energy landscapes of natively unfolded proteins.

259 ion of the ensemble of structures present in natively unfolded proteins.

260 ensemble of secondary structures present in natively unfolded proteins.

261 lpha-synuclein and by extrapolation to other natively unfolded proteins.

262 The synucleins are natively unfolded proteins.

263 sibility in fibrin fibers is mediated by the natively unfolded regions of the molecule.

264 dulates conformational transitions between a natively unfolded state and multiple alpha-helical struc

265 rmations populated by alpha-synuclein in its natively unfolded state.

266 study demonstrates that CT is predominantly natively unfolded under physiological conditions but can

267 Barnase-GCN4 is thus a "natively unfolded" protein that uses ligand binding to s

268 hibit structural characteristics typical of "natively unfolded" proteins (highly flexible proteins th

269 alpha-Synuclein has long been defined as a 'natively unfolded' monomer of about 14 kDa that is belie

270 hibition domain is intrinsically disordered (natively unfolded) as shown by circular dichroism spectr

271 that Tax(59-98) is intrinsically disordered (natively unfolded) in isolation and adopts an ordered co

272 s) is highly negatively charged and probably natively unfolded, it could anchor gravin to the inner l

273 Cytosolic alpha-synuclein appears to be natively unfolded, whereas membrane-bound alpha-synuclei

274 ssociated protein that is highly soluble and natively unfolded.

275 The P protein amino-terminal domain is natively unfolded; to probe its conformational flexibili

276 Natively unstructured (also dubbed intrinsically disorde

277 ons: regular protein secondary structure and natively unstructured (intrinsically disordered) regions

278 The amphipathicity of the natively unstructured amyloid-beta (Abeta40) peptide may

279 relationship between the transformation from natively unstructured assembly to the beta-sheet oligome

280 ation and cause of the transformation from a natively unstructured assembly to the beta-sheet oligome

281 In solution, alpha-synuclein is natively unstructured but assumes an alpha-helical confo

282 ted of RraA in complex with a portion of the natively unstructured C-terminal tail of RhlB at 2.8-A r

283 ns at random coil rates, consistent with its natively unstructured condition.

284 (RTX) domains, calcium binding triggers the natively unstructured domain to adopt a beta roll struct

285 changes, consistent with these ligands being natively unstructured in the absence of beta-catenin.

286 tuated distally and dynamically because of a natively unstructured linker connecting the dimerization

287 virus 3AB is a 109-amino acid protein with a natively unstructured N-terminal domain.

288 (EMA), protein structural domain boundaries, natively unstructured or disordered regions in proteins,

289 Human alpha-synuclein (alpha-Syn) is a natively unstructured protein whose aggregation into amy

290 rmational properties of the growing class of natively unstructured proteins in biological processes.

291 stics of several types of complexes, such as natively unstructured proteins, ribosomal proteins, two-

292 Our analysis makes use of the fact that natively unstructured proteins, which undergo a disorder

293 contains a central folded domain flanked by natively unstructured regions required to facilitate mat

294 contacts has been previously used to predict natively unstructured regions.

295 NF90, a tandem pair of dsRBDs separated by a natively unstructured segment confers dsRNA binding acti

296 f roughly a dozen residues that lie within a natively unstructured sub-domain of RNase E.

297 of distributed interactions mediated by the natively unstructured termini of Hfq.

298 ge portion of the protein is predicted to be natively unstructured, in reconstituted systems PopZ can

299 conformation, despite being predicted to be natively unstructured.

300 microfluidic systems and photovoltaics that natively use silicon materials, while mitigating adverse