ライフサイエンスコーパス: nisin

1 rs a new cell, before it encounters external nisin.

2 d rings, rings A and B, at the N-terminus of nisin.

3 he NisB protein in the maturation process of nisin.

4 e respects I30W nisin A is similar to native nisin.

5 f NisI interacts with membranes but not with nisin.

6 spores, became susceptible to inhibition by nisin.

7 Nisin(2) inserts into the membrane and we propose this a

8 active packaging was produced by compounding nisin (3, 6 and 9%) and nisin-ethylenediaminetetraacetic

9 the potential of complexation to encapsulate nisin (5g/L concentration) using spray-drying technique

10 whereas with the engineered variants, [Trp30]nisin A and [Lys27,Lys31]nisin A, the proportion of pept

11 antimicrobial activity was less potent than nisin A and H, and we propose a link between this reduce

12 in a faecal fermentation in comparison with nisin A and H.

13 inhibitory concentration (MIC) with that of nisin A and its antimicrobial effect in a faecal ferment

14 N-Terminal rings A and B of nisin A and mutacin 1140 (lipid II-binding domain) share

15 Mutacin 1140 and nisin A are peptide antibiotics that belong to the lanti

16 Nisin A binds lipid II and thus disrupts cell wall synth

17 ntly in the mutacin 1140 complex than in the nisin A complex.

18 05) in method precision for determination of nisin A in all four types of cheese.

19 ndard procedure for accurate quantitation of Nisin A in cheese becomes available.

20 Significant losses (p < 0.05) for Nisin A in cheese was observed one week later.

21 ble analytical tool for assay development of nisin A in cheese.

22 s) as internal standard for determination of nisin A in cream cheese, mascarpone, processed cheese an

23 In these respects I30W nisin A is similar to native nisin.

24 formation may be attributed to the fact that nisin A uses lipid II to form a distinct pore complex, w

25 While the K12L nisin A variant elicited a higher increase in membrane c

26 ical conductance than I30W nisin A, the H27K nisin A variant elicited weaker effects.

27 By contrast, the I30W nisin A variant induced a time-dependent reduction in li

28 re also engineered to generate K12L and H27K nisin A variants, both of which were similar to I30W nis

29 Circular complexes of mutacin 1140 and nisin A were observed by electron microscopy, providing

30 variants, both of which were similar to I30W nisin A with respect to an overall reduction in phosphol

31 citance and electrical conductance than I30W nisin A, the H27K nisin A variant elicited weaker effect

32 ed variants, [Trp30]nisin A and [Lys27,Lys31]nisin A, the proportion of peptide that escapes full pro

33 obacillin I was generally similar to that of nisin A, with increased activity against Streptococcus d

34 onstrated that the mutacin 1140-lipid II and nisin A-lipid II complexes are very stable and capable o

35 demonstrated increased stability compared to nisin A.

36 lipids were coupled to the C-terminus of the nisin A/B ring system to generate semisynthetic construc

37 ce, we tested mutacin IV (a nonlantibiotic), nisin (a single peptide lantibiotics), and three peptide

38 Nisin, a 34 residue lantibiotic produced by strains of L

39 circumvented with a combined approach using nisin, a FDA-approved safe bacteriocin, to inhibit outgr

40 Nisin, a model lantibiotic, has a dual mode of action: i

41 We also found that nisin, a monopeptide lantibiotic, requires LsrS for its

42 Nisin, a polycyclic antibacterial peptide, is effective

43 creting a lantibiotic that is similar to the nisin-A produced by Lactococcus lactis.

44 In comparison to nisin-A, the BP(SCSK) lantibiotic has reduced activity a

45 ontext of other recently proposed models for nisin action.

46 Expression of aggregation substance after nisin addition to cultures of E. faecalis and the hetero

47 correlation between cell membrane damage and nisin aggregation was observed in vivo.

48 in complexes, it favored the dissociation of nisin-alginate aggregates to form individual complexes.

49 Spray-drying of nisin-low methoxyl pectin or nisin-alginate electrostatic complexes has led to the mi

50 2)P-Labeled cell wall anchor species bind to nisin, an antibiotic that is known to form a complex wit

51 In the present study, nisin, an antimicrobial agent, was encapsulated in essen

52 Herein, we describe a nisin analog encoded on the genome of the thermophilic b

53 Nisin and EDTA interacted with polymers, involving CO st

54 ificant decreases in resistance to the CAMPs nisin and gallidermin but not polymyxin B.

55 st, the C-terminal domain specifically binds nisin and modulates the membrane affinity of the N-termi

56 Nisin and natamycin are natural food preservatives exten

57 nd chromatographic techniques for monitoring nisin and natamycin levels for regulatory compliance and

58 techniques are required for the analysis of nisin and natamycin.

59 ly halloysite, are suitable nanocarriers for nisin and pediocin adsorption.

60 Higher adsorption of nisin and pediocin was obtained on bentonite.

61 tial carriers for the antimicrobial peptides nisin and pediocin.

62 bacterial lipolytic enzymes, the effects of nisin and PLA2 on the degradation of S. aureus lipids we

63 h two chimeric peptides, suggesting that the nisin and prochlorosin biosynthetic enzymes can produce

64 t of CSP but depended on treatment with both nisin and raffinose, showing that coexpression of comW a

65 The lantibiotic nisin and related peptides display unique and highly sel

66 cyclase enzymes involved in the synthesis of nisin and subtilin (NisC and SpaC, respectively) have be

67 negative bacteria; and like the lantibiotics nisin and subtilin in its ability to inhibit both bacter

68 two more than the five cross-links found in nisin and the most cross-links found in any lantibiotic

69 st-resistant mutants were cross-resistant to nisin and the pAD1-encoded cytolysin.

70 Starch-OS based emulsions not only retained nisin and thymol activities separately, but also exhibit

71 to evaluate the antimicrobial activities of nisin and thymol formulations in cantaloupe juice.

72 succinate (starch-OS) were used to stabilize nisin and thymol in cantaloupe juice-containing fluid.

73 lsions had much greater capability to retain nisin and thymol over the storage and displayed much gre

74 Native nisin and variants elicit an enhanced release of calcein

75 antibiotics, such as vancomycin, ramoplanin, nisin, and bacitracin.

76 n of Fst sensitized cells to the lantibiotic nisin, and Fst-resistant mutants were cross-resistant to

77 The genes encoding nisin are organized in three contiguous operons: nisABTC

78 In this work, the use of nisin as a biological molecule for the development of a

79 By using a nisin biosynthesis pathway and its variants as examples,

80 This feature of nisin biosynthesis was exploited in an investigation of

81 responsible for the cyclization step during nisin biosynthesis.

82 riments, we found that fluorescently labeled nisin bound very inhomogeneously to bacterial membranes

83 LanI protein NisI provides immunity against nisin but not against structurally very similar lantibio

84 ynergistic effects of osmotic activation and nisin bypass the limitations of germination as a spore c

85 nt immunity (NisI) can be expressed when the nisin cluster enters a new cell, before it encounters ex

86 The lipid II-binding N-terminus of nisin, comprising the so-called A/B ring system, was syn

87 ipid II aggregates as a function of time and nisin concentration.

88 se synthesis of an analogue of a fragment of nisin containing its ring C.

89 As with all lantibiotics, nisin contains a number of dehydro-residues and thioethe

90 Nisin contains dehydroalanine and dehydrobutyrine residu

91 Nisin contains the unusual amino acids dehydroalanine an

92 ased structures can be valuable carriers for nisin delivery in food systems.

93 vely block the antibacterial activity of the nisin-derived lipopeptides.

94 cases, the serine residue, at position 33 of nisin, does not undergo dehydration to Dha33.

95 ged residues in controlling ion flow through nisin-doped membranes.

96 Interaction between nisin, EDTA and polymers modified the morphology and fil

97 PBAT/TPS films containing EDTA and nisin effectively inhibited lipid degradation in pork ti

98 icroemulsions were formulated to encapsulate nisin enhancing the system's overall antimicrobial activ

99 duced by compounding nisin (3, 6 and 9%) and nisin-ethylenediaminetetraacetic acid (EDTA) (3 and 6%)

100 Here we show that the lantibiotic nisin exercises its antibacterial action by targeting pe

101 The antimicrobial peptide nisin exerts its activity by a unique dual mechanism.

102 N-Terminal nisin fragments N1-12 and N1-20 had little effect on pho

103 is likely an evolutionary adaptation of the nisin gene cluster to enable its successful establishmen

104 their band widths specified by the external nisin gradient and cellular nisin immunity.

105 Nisin has attracted much attention recently due to its n

106 Nisin has been commercially used as a food preservative,

107 An Ile1Trp mutation of the N-terminus of nisin has been modelled and docked onto lipid II models;

108 The peptide natural product nisin has been used as a food preservative for 6 decades

109 The lantibiotic nisin has been used as an effective food preservative to

110 as it provides little protection against the nisin hinge region variant.

111 These activities can be separated in a nisin hinge-region variant (N20P M21P) that binds lipid

112 ated for the D-Cys3, D-Cys7, L-Cys8, L-Cys11 nisin homologue, while interlinked rings A and B are obt

113 by the external nisin gradient and cellular nisin immunity.

114 nalog ethionine (instead of methionine) into nisin improves its bioactivity against several Gram-posi

115 propose a model of antibacterial action for nisin in which the terminal amino group of Ile1 targets

116 is operon was upregulated in the presence of nisin in wild-type cells and was more highly expressed i

117 Two diastereomeric analogues of ring C of nisin incorporating a novel norlanthionine residue have

118 f fosfomycin, d-cycloserine, vancomycin, and nisin, indicating a wide-spectrum hypersensitivity to in

119 Structural changes of nisin induced by complexation with pectin or alginate an

120 pCF10, was cloned in a vector containing the nisin-inducible nisA promoter and its two-component regu

121 Enhanced Pel expression from a nisin-inducible plasmid resulted in increased message le

122 opy plasmid pMSP3535, under the control of a nisin-inducible promoter (P(N)), and transformed into pn

123 1 lysin and holin genes were cloned into the nisin-inducible shuttle vector pMSP3545, nisin induction

124 tose induction and significantly reduced the nisin induction capacity, suggesting a potential pivotal

125 the nisin-inducible shuttle vector pMSP3545, nisin induction of holin and lysin caused partial lysis

126 ch was identical to the nisA start site upon nisin induction.

127 In contrast, neither vancomycin nor nisin inhibited outgrowth for the 012 ribotype.

128 olution, osmotic activation solutes enhanced nisin inhibition of superdormant spores to >3.5 log(10)C

129 Nisin is a complex lanthipeptide that has broad spectrum

130 Nisin is a polymacrocyclic peptide antimicrobial with hi

131 Nisin is a post-translationally modified antimicrobial p

132 Nisin is a posttranslationally modified antimicrobial pe

133 The lantibiotic nisin is an antimicrobial peptide produced by Lactococcu

134 The lantibiotic nisin is an antimicrobial peptide that is widely used as

135 Nisin is an antimicrobial peptide with bacterial, fungic

136 The best-known, nisin, is a commercial food preservative.

137 growth, with activities approaching that of nisin itself.

138 cused on the biosynthesis of the antibiotics nisin, lacticin 481, fosfomycin, and bialaphos.

139 ules for the formation of defined and stable nisin-like pores, however, slow membrane depolarization

140 Lipid II mobility due to a steady growth of nisin-Lipid II aggregates as a function of time and nisi

141 n to Lipid II induces the formation of large nisin-Lipid II aggregates in the membrane of bacteria as

142 ore, we observed that the formation of large nisin-Lipid II aggregates induced vesicle budding in gia

143 hat is dependent on the continuous growth of nisin-Lipid II aggregation and probably involves curvatu

144 A key feature of the nisin-lipid II interaction is the formation of a cage-li

145 wever, structural and mechanistic details on nisin-lipid II membrane complexes are currently lacking.

146 as been put forward as the building block of nisin/lipid II binary membrane pores.

147 fferent lipid II structures and a stable 2:1 nisin:lipid II complex formed.

148 Subsequently, nisin-loaded microemulsions were tested for their antimi

149 information on the antibacterial activity of nisin-loaded nano-carriers enhanced by essential oils, i

150 Spray-drying of nisin-low methoxyl pectin or nisin-alginate electrostati

151 ation of the role of the NisB protein in pre-nisin maturation.

152 ctrostatic attraction encourages the initial nisin-membrane association.

153 t reduction in lipid mobility, indicative of nisin-membrane surface interactions, as well as a declin

154 iosensor for bacteria detection is reported: nisin molecules were immobilised on gold electrodes and

155 irst step in pore formation, mediated by the nisin N-terminus-lipid II pentapeptide hydrogen bond.

156 effect of SDP on cells differs from that of nisin, nigericin, valinomycin and vancomycin-KCl, but re

157 Nisin (NIS) Z was incorporated (0.05 %, 0.1 %, 0.2 %) in

158 in the biosynthesis of the food preservative nisin (NisB).

159 n-based nanofibers (NFs) functionalized with nisin (NS), reinforced with montmorillonite nanoclay (nM

160 the coculture system to assess the effect of nisin on A498 kidney cancer cells cocultured with bacter

161 lipidated variants at different positions in nisin or in truncated nisin variants.

162 s the homogenous form of the fully processed nisin (or nisin variant) molecule.

163 Nisin P is a natural nisin variant, the genetic determin

164 Nisin P was purified, and its predicted structure was co

165 gut and food isolates by virtue of producing nisin P.

166 it to expand the phenotypic diversity of the nisin pathway by quickly generating a library of 63 path

167 y by altering the regulatory topology of the nisin pathway for constitutive bacteriocin biosynthesis.

168 ile spray-drying promoted the aggregation of nisin-pectin complexes, it favored the dissociation of n

169 wth inhibition curves revealed that LMWC and nisin possessed inverse antibacterial activity against t

170 en when the gradient is driven by structured nisin-producing bacteria and the patterning cells are co

171 id-encoded nisB gene in a range of different nisin-producing strains.

172 Low molecular weight chitosan (LMWC) and nisin, recognized as cationic antibacterial agents (CAAs

173 crocycles A and B within cysteine mutants of nisin residues 1-12 is investigated here by molecular dy

174 sigma(W) contributes to nisin resistance by regulation of a signal peptide pepti

175 The major contribution of sigma(M) to nisin resistance is expression of ltaSa, encoding a stre

176 the presence of extracellular bacitracin and nisin, respectively, the two response regulators (RRs) b

177 Nisin retained the antimicrobial activity against all mi

178 Despite this successful use, nisin's stability at pH 7 is limited.

179 We show that nisin sequesters cell wall precursors found in the outer

180 he dual signaling and antibiotic features of nisin, simple synthetic circuits can direct Lactococcus

181 r alginate (2g/L concentration) can preserve nisin structure and antimicrobial activity.

182 mplexation with pectin or alginate preserved nisin structure as well as its antimicrobial activity du

183 sigX and dltA mutants are more sensitive to nisin than wild-type cells.

184 pare individual ring A and B structures from nisin, the related lantibiotic mutacin, and synthetic an

185 With native nisin this partially processed form represents about 10%

186 ell envelope structure to decrease access of nisin to its lipid II target.

187 Binding of nisin to Lipid II induces the formation of large nisin-L

188 In contrast to nisin treatment, PLA2 treatment does not stimulate endog

189 genous form of the fully processed nisin (or nisin variant) molecule.

190 Nisin P is a natural nisin variant, the genetic determinants for which were p

191 we now report the recombinant expression of Nisin variants that incorporate noncanonical amino acids

192 ning ncAA that allowed for the expression of Nisin variants with novel macrocyclic topologies.

193 different positions in nisin or in truncated nisin variants.

194 nd nisFEG requires autoinduction by external nisin via signal transducing by NisRK.

195 Wild-type nisin was also docked onto three different lipid II stru

196 of the deletion derivatives by galactose and nisin was compared phenotypically using beta-galactosida

197 Nisin was encapsulated in silica through sol-gel process

198 A twofold reduction in the MIC value of nisin was obtained against S. aureus, inoculated in a 1.

199 gate the aggregation process of Lipid II and nisin, we assessed its dynamics by single-molecule micro

200 the bacterium on medium containing the CAMP nisin, we isolated a mutant capable of growing in three

201 ic effects of osmotic activation solutes and nisin were associated with loss of membrane integrity.

202 The structural aspects of nisin which facilitate membrane interaction and permeabi

203 actis produce the broad-spectrum bacteriocin nisin, which belongs to the lantibiotic class of antimic

204 ystem secretes polypeptide molecules such as nisin, which has been reported to trigger cell apoptosis

205 Lactococcus lactis produces the lantibiotic nisin, which is widely used as a food preservative.

206 d different conformations in the presence of nisin, which may also have implications for pore formati

207 x, specifically the N-terminal engagement of nisin with lipid II at the pyrophosphate and C-terminus