ライフサイエンスコーパス: notochord

1 ith one key issue being the emergence of the notochord.

2 ad initiated by Hedgehog signalling from the notochord.

3 on and stiffening, gave rise to the chordate notochord.

4 t the zebrafish embryo with exception of the notochord.

5 ession of cEbf1 is regulated by Shh from the notochord.

6 cEbf1 is controlled by Shh signals from the notochord.

7 for specification and differentiation of the notochord.

8 ltimately leads to bifurcation of the caudal notochord.

9 dary tail containing both somitic muscle and notochord.

10 urchin, and the nematode and in the chordate notochord.

11 age and other cartilage-like tissues such as notochord.

12 geckos reverted to the ancestral persisting notochord.

13 floor plate and inhibits contribution to the notochord.

14 equired for ntl expression in the developing notochord.

15 contribute to either the floor plate or the notochord.

16 e kidney fusion, even in the presence of the notochord.

17 Xbp1 and Creb3l2 were also activated in the notochord.

18 ssed but not differentially activated in the notochord.

19 of the intervertebral disc develops from the notochord.

20 th retardation and incomplete closure of the notochord.

21 tive streak markers overlies the prospective notochord.

22 morphological boundary around the developing notochord.

23 he first time expression in the eyes and the notochord.

24 f the brain along with an enlargement of the notochord.

25 he presumptive epidermis and surrounding the notochord.

26 ate volumetric growth in the spinal cord and notochord.

27 diates surprisingly strong expression in the notochord.

28 ards the midline and coalesce underneath the notochord.

29 ermal tissues in the dorsal isolate, and the notochord, a central structure involved in patterning ve

30 The notochord, a conserved axial structure required for embr

31 displays unambiguous vertebrate features: a notochord, a pair of prominent camera-type eyes, paired

32 During development of the notochord--a structure akin to the vertebrate spine--in

33 Tissue manipulations (notochord ablation) and Shh gain and loss of function ex

34 ly regulated by a change in signaling by the notochord along the anteroposterior axis.

35 ession, located at the end of the developing notochord, also known as the embryonic node and crown ce

36 lls of the NP are thought to derive from the notochord, although adult NP lacks identifiable notochor

37 lino oligonucleotide injection caused a wavy notochord and cardiovascular abnormalities with a reduce

38 col8a1a), and cause folding of the embryonic notochord and consequently adult vertebral column malfor

39 e shaped and positioned by C&E alongside the notochord and differentiate into skeleton, fast, and slo

40 Then, sheath cells invade the inner notochord and differentiate into vacuolated cells, there

41 l volume asymmetry throughout the developing notochord and find that there are distinctive patterns o

42 spinal cord levels, Shh produced by both the notochord and floor plate (FP) diffuses dorsally to orga

43 Here, we showed that disruption of the notochord and floor plate by diphtheria toxin (DTA)-medi

44 vatives that arise in close proximity to the notochord and floor plate, it has been assumed that thei

45 ties, we specifically inactivated Shh in the notochord and floor plate.

46 in some anterior basal plate domains and by notochord and floorplate cells, and ventral neural cells

47 hordate amphioxus, which expresses Hh in its notochord and floorplate, provides a window into the pre

48 centers including the dorsal marginal zone, notochord and floorplate.

49 hought to arise from transformed remnants of notochord and have a predilection for the axial skeleton

50 For example, the notochord and mesenchyme are induced by FGF/MAPK signali

51 eurula stages, followed by a failure to form notochord and muscle and then the partial loss of trunk

52 Removal of hedgehog signaling in the notochord and nearby floorplate resulted in the formatio

53 ortant for convergent extension in the mouse notochord and neural plate, the results indicate that ch

54 ibutes descendants to the paraxial mesoderm, notochord and neural tube, and is serially transplantabl

55 shment of asymmetric cell fates in the Ciona notochord and neural tube.

56 m traveling across the anterior limit of the notochord and propagating down the right LPM.

57 tes in having a dorsal, hollow nerve cord, a notochord and somites.

58 insights into the mechanisms that safeguard notochord and spine development.

59 anterior streak derivatives namely the node, notochord and the emerging definitive endoderm.

60 plate, while cell fate specification of the notochord and the floor plate, as well as signaling with

61 rom Hensen's node of the chick embryo to the notochord and the floor plate.

62 s were then performed to analyse whether the notochord and/or Shh regulate cEbf1 expression.

63 ryos with hydrocephaly and abnormally curved notochords and overall body shape, whereas published kno

64 the vertebrate dorsal midline (floor plate, notochord, and hypochord) has been an area of classical

65 y localized to the presumptive epidermis and notochord, and play a critical and unexpected role in po

66 es involved in locomotion, including muscle, notochord, and the central nervous system.

67 Interestingly, the notochord appears to be the only embryonic structure in

68 The development of the notochord appears to have a major effect on the surround

69 definitive endoderm, as well as the node and notochord, arises at the same time but mostly in cells t

70 RA, which marks the early mesoderm, node and notochord, arises in Oct4 expressing cells on days 3-4.

71 We further identify the mouse notochord as a rich source of multiple redundant neurona

72 Moreover, the Ciona notochord as a single-file array of forty polarized cell

73 mesodermal derivatives including muscle and notochord, as well as within the nervous system.

74 e as vertebral bone formation compresses the notochord asymmetrically, causing vertebral malformation

75 The disruption of the notochord band has been observed with IVD degeneration.

76 Furthermore, Noggin mimics notochord-based inhibition by preventing mesodermal EC g

77 n of histones within a group of cells in the notochord bead and for promoting wound-induced prolifera

78 displaying citrullinated histones within the notochord bead following tissue injury.

79 al use of these mechanisms between different notochord blastomeres and also between different rounds

80 ediolateral intercalation but forms a robust notochord boundary.

81 isense morpholinos led to strong deficits in notochord but not somitic muscle development.

82 romotes floor plate and hypochord fates over notochord, but has variable effects on Shh expression in

83 secretory pathway genes was observed in the notochord, but not in notochord precursors in the axial

84 set of axial mesoderm that gives rise to the notochord, but not prechordal mesoderm, which gives rise

85 de, an arrangement which is seen in ascidian notochords, but which has not been observed in other mod

86 Finally, we show that bends and kinks in the notochord can lead to aberrant apposition of osteoblasts

87 ic development of mesoderm-derived posterior notochord, cardiac, and hematopoietic tissues.

88 We describe new evidence for a notochord, cartilaginous arcualia, gill pouches, articul

89 During aortae fusion, the notochord ceases to exert its negative influence on vess

90 to drive medial precursor cells towards the notochord cell fate.

91 sibling cell volume asymmetries that precede notochord cell intercalation; the developmental timing o

92 ds the middle; and the differential rates of notochord cell narrowing after intercalation.

93 n of the embryonic axis, as well as abnormal notochord cell polarity.

94 This tapered shape involves differences in notochord cell volume along the anterior-posterior axis.

95 lain all the anterior-posterior variation in notochord cell volume.

96 s, which consist of variable dislocations of notochord cells along the anterior-posterior axis.

97 e segments containing transplanted wild-type notochord cells but not in the ones containing wild-type

98 small nuclei pulposi were formed, with most notochord cells dispersed throughout the vertebral bodie

99 As notochord cells form nuclei pulposi, we propose that the

100 Here, we perform RNAseq on flow-sorted notochord cells from multiple stages to define a compreh

101 chord extension through PCP-controlled CE of notochord cells, establishing a role for Wnt11 in mammal

102 ation of polarized actin-rich protrusions in notochord cells, resulting in defective notochord interc

103 rm cells and encase the inflating vacuolated notochord cells.

104 ents are unable to activate transcription in notochord cells.

105 d low-level ShhN in the prechordal plate and notochord, consistent with the notion that ShhN can rapi

106 P) position, with cells in the middle of the notochord consistently wider than cells at the anterior

107 Serial deletions of the 581-bp notochord CRM revealed that this sequence is also able t

108 requires Ci-Bra, and identified a Ci-Tbx2/3 notochord CRM that necessitates multiple Ci-Bra binding

109 and FoxA in the activation of an individual notochord CRM, and highlights the importance of transcri

110 tic characterization of Brachyury-downstream notochord CRMs.

111 ignaling is not required for floor plate vs. notochord decisions and plays a minor role in floor plat

112 can, knockdown of Fukutin or FKRP leads to a notochord defect and a perturbation of laminin expressio

113 of individual zebrafish, we correlate focal notochord defects of the embryo with vertebral malformat

114 These data suggest that signals from the notochord define the evolutionary identity of the spine

115 hat the lacZ insertions interfered with some notochord-dependent aspect of vertebral development.

116 We find that cell shape in the intercalated notochord depends strongly on anterior-posterior (AP) po

117 function affects only development of caudal notochord derivatives and is compensated for in its othe

118 e marker, downstream to and regulated by the notochord derived Shh, which may be functionally involve

119 e to ECs and to form blood vessels, but that notochord derived-BMP antagonists suppress EC differenti

120 During development, embryonic notochord-derived cells (NDCs) are the direct progenitor

121 tiation of the endocardial progenitors while notochord-derived Hh is a likely source for the specific

122 rectly observe, measure, localize and modify notochord-derived Shh ligand in the context of neural pa

123 Since notochord-derived signals are essential for formation of

124 Notochord-derived Sonic Hedgehog (Shh) is essential for

125 This work shows that this notochord-derived tissue continues to carry out a major

126 Surrounding tissues, such as the notochord, dermomyotome, and sclerotome also exhibit dif

127 We specifically focus on the processes of notochord development and cerebrospinal fluid physiology

128 he T (brachyury) gene, which is important in notochord development and is expressed in most sporadic

129 class transcription factors is required for notochord development.

130 ession and nutrient availability critical to notochord development.

131 In vertebrates, the notochord develops by convergence and extension of the c

132 otochord precursor pool is depleted, and the notochord differentiates prematurely.

133 Notochord differentiation and morphogenesis are severely

134 nchymal PSM while blocking somitogenesis and notochord differentiation.

135 We also report that the notochord diminishes the ability of mature ECs to organi

136 The Ciona notochord displays planar cell polarity (PCP), with ante

137 The notochord does not contact the TVCs but contributes to t

138 constituents, defining the structure of the notochord during aging is critical for investigations re

139 or maintaining the rod-like structure of the notochord during early embryonic development.

140 alized expression of pld1 is observed in the notochord during early segmentation, in the somites duri

141 odules that begin with the elongation of the notochord during gastrulation in the rapidly developing

142 ation was supported by the identification of notochord enhancers enriched upstream of the KIAA0319 tr

143 high-throughput screen identified synthetic notochord enhancers that are activated by the combinatio

144 ode enabled in silico discovery of bona fide notochord enhancers, including those containing low-affi

145 (WGEF) gene by a microarray-based screen for notochord enriched genes, and show that WGEF is involved

146 find only modest overlap between this set of notochord-enriched transcripts and the genes upregulated

147 l be invaluable for testing hypotheses about notochord evolution.

148 This set of putative effector genes with notochord expression conserved from tunicates to vertebr

149 112 of the Ciona notochord genes have known notochord expression in vertebrates, more than twice as

150 At the same time, notochord expression is maintained after Sox9a knockdown

151 We determined that the notochord expression of Ciona Tbx2/3 (Ci-Tbx2/3) require

152 Both genes regulate trunk notochord extension through PCP-controlled CE of notocho

153 Mouse embryos that lack notochords fail to form cohesive aortic vessels because

154 posterior structures, including neural tube, notochord, fin, and muscle.

155 ls never give rise to midline tissues of the notochord, floor plate and dorsal endoderm, raising the

156 tain the axial identity of prechordal plate, notochord, floor plate and hypochord progenitors during

157 r expression in craniofacial cartilage, ear, notochord, floor plate, hypochord and fins in a pattern

158 ing induces notochord within a population of notochord/floor plate bipotential cells through negative

159 ryos and acts as a Wnt antagonist to promote notochord formation and prevent muscle differentiation.

160 anscription factor Brachyury is required for notochord formation in all chordates, and that it contro

161 lts in absent melanin pigmentation, impaired notochord formation, and hindbrain neurodegeneration.

162 er stages in development ntl is required for notochord formation, and our analysis has also led to th

163 of the neural tube and somites by regulating notochord formation, and provide evidence that both gene

164 Blastopore closure, notochord formation, somite development, neural tube clo

165 is a characteristic and necessary feature of notochord formation.

166 e in all 4 bilateral pairs of A-line primary notochord founder cells and also in the B-line-derived s

167 lls and also in the B-line-derived secondary notochord founder cells.

168 er sufficient resolution to discriminate the notochord from the surrounding the nucleus pulposus, esp

169 ate into vacuolated cells, thereby restoring notochord function and allowing normal spine development

170 se axial defects do not arise from perturbed notochord function, as cellular proliferation, apoptosis

171 gulated biogenesis of notochord vacuoles and notochord function.

172 es a foundation for systems-level studies of notochord gene regulation and morphogenesis.

173 are complicated by the limited knowledge of notochord genes and cis-regulatory modules (CRMs) that a

174 Orthologs of 112 of the Ciona notochord genes have known notochord expression in verte

175 The full set of Ciona notochord genes provides a foundation for systems-level

176 specifier Macho-1 and 50 Brachyury-regulated notochord genes, as well as several anti-neural factors

177 f which overlap with known Ci-Bra-downstream notochord genes.

178 The notochord gives rise to spinal segments during developme

179 During later mouse embryogenesis, the notochord gives rise to the middle part of the intervert

180 The assessment and imaging of the notochord has classically relied on histological techniq

181 and cause developmental defect in the brain, notochord, heart, and kidney, depending on the delivery

182 ent at the dorsal midline, prechordal plate, notochord, hypochord and floor plate share a common embr

183 t in the tailbud to specify hypochord from a notochord/hypochord bipotential cell population.

184 formation of the posterior mesoderm and the notochord in vertebrate embryos.

185 emonstrate that morphological defects of the notochord in zebrafish can generate congenital-type spin

186 The role of axial structures, especially the notochord, in metanephric kidney development has not bee

187 Sonic hedgehog (Shh) ligand secreted by the notochord induces distinct ventral cell identities in th

188 n1b, we show that caveolae are essential for notochord integrity.

189 of FGF3 in the developing nerve cord directs notochord intercalation through non-MAPK signaling.

190 s in notochord cells, resulting in defective notochord intercalation.

191 The notochord is a defining feature of the chordate body pla

192 At the center of the vertebrate notochord is a large fluid-filled organelle, the notocho

193 e of the developing neural tube, whereas the notochord is a rod of axial mesoderm that lies directly

194 The vertebrae notochord is a transient rod-like structure that produce

195 The notochord is considered an evolutionary novelty and one

196 These results suggested that the notochord is dispensable for nephrogenesis but required

197 ence that function of Pld1 in the developing notochord is essential for vascular development in verte

198 The notochord is necessary for the development of the chorda

199 y cascading through the entire length of the notochord is not supported; instead a more complex mecha

200 is morphological feature of having a tapered notochord is present in many chordates.

201 ehog (Shh) secreted from the floor plate and notochord is required for specification of ventral (audi

202 We have previously shown that the notochord is responsible for the generation and maintena

203 signaling) secreted from the midline by the notochord, it is unknown how fusion is later signaled.

204 l stage such that, by the tadpole stage, the notochord lacks any recognizable structure, although not

205 approach to guide ESC differentiation into a notochord-like population.

206 Some cells misdirected from the notochord lineage were found to be incorporated into def

207 In E- embryos, the collagen sheath notochord markers (col2a1a and col9a2) appeared bent.

208 d lacks any recognizable structure, although notochord markers are expressed in a normal temporal pat

209 rachyury, indicating that Brachyury is not a notochord master regulator gene as strictly defined.

210 cellular analysis suggests that Shh from the notochord might traffic into the neural target field by

211 le of DSTYK in notochord vacuole biogenesis, notochord morphogenesis and spine development through mT

212 tropicalis, with prominent expression in the notochord, nervous system and stomach.

213 rapidly developing frogs, elongation of the notochord occurred earlier relative to the time point of

214 o assay based on misexpressing Ci-MRF in the notochord of Ciona embryos.

215 2 targeting a gene that is expressed in the notochord of early embryos and in multiple epithelia dur

216 The notochord of the ascidian Ciona consists of only 40 cell

217 The notochord of the ascidian Ciona provides a unique model

218 es in the development of a simple organ: the notochord of the ascidian Ciona savignyi.

219 The notochord of the invertebrate chordate Ciona forms a tap

220 sential regulator of tubulogenesis using the notochord of the invertebrate chordate Ciona intestinali

221 compared the transcriptome in the developing notochord of Xenopus laevis embryos with that of other e

222 oderm is nearly twice as stiff as either the notochord or neural plate, and at least 10-fold stiffer

223 cal basis for zebrafish and mouse bifurcated notochord phenotypes as well as the rare human congenita

224 the simple chordate Ciona intestinalis, the notochord plate consists of just 40 cells, which undergo

225 es, and review evidence in teleosts that the notochord plays an instructive role in segmental pattern

226 The notochord plays critical structural and signaling roles

227 Thus, the vertebrate notochord plays important structural roles beyond early

228 The leftward nodal flow across the posterior notochord (PNC) has been identified as the earliest even

229 f ciliated cells of the mouse COA (posterior notochord, PNC), we can restore fluid flow, asymmetric e

230 In the absence of Bmp4, the notochord precursor pool is depleted, and the notochord

231 es was observed in the notochord, but not in notochord precursors in the axial mesoderm at early gast

232 m-ectoderm border, decreased adhesion at the notochord-presomitic mesoderm border, and tension at bou

233 ost abundant BMP antagonist expressed in the notochord prior to fusion, undergoes a dramatic downregu

234 genesis, possibly by maintaining cohesion of notochord progenitors by regulation of cadherin localiza

235 uromesodermal, lateral/paraxial mesoderm and notochord progenitors; NMPs, LPMPs and NotoPs).

236 the mutation implicated the teleost-specific notochord protein, Calymmin, as a key regulator of spine

237 s work sheds light on a large section of the notochord regulatory circuitry controlled by T-box facto

238 heir contributions to the development of the notochord remain uncharacterized.

239 ic domains, we describe the structure of the notochord remnants with aging in the lumbar IVDs of BALB

240 The appearance of the notochord represented a milestone in Deuterostome evolut

241 ation of polarity along the long axis of the notochord requires the PCP pathway, a role we demonstrat

242 mutant forms of Ci-Tbx2/3 in the developing notochord revealed a role for this transcription factor

243 rtebral column or spine assembles around the notochord rod which contains a core made of large vacuol

244 ed and then restored, underscoring the Ciona notochord's amenability for in vivo studies of PCP.

245 in compression of the axial neural tube and notochord; second, elongation of axial tissues driven by

246 ebral soft tissue types (cartilage, probable notochord) seen in extant reptiles.

247 lement, we analyzed the morphogenesis of the notochord sheath cells as they withdraw from the stack o

248 Here, we show that increased notochord sheath collagen deposition in polycystin 2-def

249 mutants is related to the wavy and malformed notochord sheath formation and abnormal axial skeleton s

250 lls form nuclei pulposi, we propose that the notochord sheath functions as a "wrapper" around the not

251 The notochord sheath mineralizes normally, supporting the id

252 Our data suggest that the formation of the notochord sheath requires hedgehog signaling and that th

253 The mutation in cmn results in loss of notochord sheath segmentation, altering osteoblast migra

254 ate resulted in the formation of an aberrant notochord sheath that normally surrounds this structure.

255 In the absence of the notochord sheath, small nuclei pulposi were formed, with

256 , apoptosis, and expression of regulators of notochord signaling are normal in Pbx1/Pbx2 mutants.

257 The timing of notochord, somite, and neural development was analyzed i

258 l reveals a statistical enrichment of Dvl in notochord-somite boundary-(NSB)-directed protrusions, wh

259 Ciona, in which the single-copy Brachyury is notochord-specific and CRMs are easily identifiable, to

260 ctivity, we have identified a 581-bp minimal notochord-specific cis-regulatory module (CRM) whose act

261 d extends toward the midline and expresses a notochord-specific combination of genes.

262 le model for such investigations in a 155-bp notochord-specific CRM from the ascidian Ciona intestina

263 ry code" of sequence and syntax features for notochord-specific expression.

264 , leprecan was identified as a target of the notochord-specific transcription factor Ciona Brachyury

265 tes Brachyury (Ci-Bra), a key determinant of notochord specification.

266 attern to kiaa0319, was not expressed in the notochord suggesting a distinct role for kiaa0319 in thi

267 ed by a paracrine input of Tgfbeta3 from the notochord, suggesting that the former programs the hemog

268 Here we report that the notochord suppresses the generation of ECs from the meso

269 s as well as the rare human congenital split notochord syndrome.

270 We find that ascidian notochord taper involves three main mechanisms: Planar C

271 Among the Ci-Tbx2/3 notochord targets are evolutionarily conserved genes, in

272 In some tissues such as lamprey notochord, the collagen fibrillar organization is natura

273 populate not only neurectoderm, somites and notochord throughout the axis, but also the chordoneural

274 diffraction data from native and derivative notochord tissue samples to solve the axial, D-periodic

275 rant bone deposition at regions of misshapen notochord tissue.

276 We also reveal polarity in the notochord to be dynamic: a cell's polarity state can be

277 d sheath functions as a "wrapper" around the notochord to constrain these cells along the vertebral c

278 tal day 0, representing opposite ends of the notochord to NP transformation.

279 Midline progenitors can be transfated from notochord to somite fate after gastrulation by ectopic e

280 upregulated by ectopic expression of the key notochord transcription factor Brachyury, indicating tha

281 tiple stages to define a comprehensive Ciona notochord transcriptome.

282 Dstyk knockdown inhibits notochord vacuole and lysosome biogenesis through mTORC1

283 of mTORC1 activity can rescue the defect in notochord vacuole biogenesis and scoliosis in dstyk muta

284 , our findings reveal a key role of DSTYK in notochord vacuole biogenesis, notochord morphogenesis an

285 chord is a large fluid-filled organelle, the notochord vacuole.

286 nase gene dstyk that causes fragmentation of notochord vacuoles and a severe congenital scoliosis-lik

287 gmentation due to dysregulated biogenesis of notochord vacuoles and notochord function.

288 We establish that notochord vacuoles are required for body axis elongation

289 Here we show that zebrafish notochord vacuoles are specialized lysosome-related orga

290 We find that localized disruption of notochord vacuoles causes vertebral malformation and cur

291 Together, our data show that notochord vacuoles function as a hydrostatic scaffold th

292 The notochord was adjacent to the dorsal foregut endoderm du

293 on of the spinal cord isolated together with notochord was used, and responses to bending were record

294 as active in specific nonneural cells of the notochord when placed with APPb gene promoter proximal e

295 r developing structures, particularly in the notochord which, is key for establishing body patterning

296 n that the NP cells arise from the embryonic notochord, which acts as a major signaling center in the

297 box sites that are utilized by Ci-Bra in the notochord, which are also bound in vitro by the muscle-s

298 while allowing expression to persist in the notochord, which underlies the neural tube during neurog

299 Mechanisms coordinating notochord-wide polarity remain elusive, but appear to en

300 Wnt signaling induces notochord within a population of notochord/floor plate b