ライフサイエンスコーパス: nuclease

1 stalled forks from degradation by the MRE11 nuclease.

2 methyltransferase, EZH2 is not known to be a nuclease.

3 gulate the activities of XPB ATPase and Bax1 nuclease.

4 ounding a constitutive, potentially harmful, nuclease.

5 SB) formation, in this case by the SLX4/SLX1 nuclease.

6 by a long-range step involving EXO1 or DNA2 nuclease.

7 roduced by RNase 1, a highly active secreted nuclease.

8 ingle-stranded DNA created by Mre11 and CtIP nucleases.

9 eaks (DSBs) after genome editing with CRISPR nucleases.

10 e and the degradation activity of endogenous nucleases.

11 eukaryotic cells possess multiple resection nucleases.

12 ded in open chromatin that are accessible to nucleases.

13 avage outcomes across engineered and natural nucleases.

14 adenylate (cA4) and deactivate the ancillary nucleases.

15 o limit Csm6 activity in the absence of ring nucleases.

16 oolset of RNA-programmable CRISPR-associated nucleases.

17 may be shared by other 5' structure-specific nucleases.

18 ions of divalent cations and the presence of nucleases.

19 d quantity of circulating tumor cell-derived nucleases.

20 res that could afford protection from plasma nucleases.

21 ge sites and coordinating damage incision by nucleases.

22 R defence DNA endonuclease, CRISPR ancillary nuclease 1 (Can1).

23 ining the DNA repair gene Fanconi-Associated Nuclease 1 (FAN1).

24 tivity and light-induced SMALL RNA DEGRADING NUCLEASE 1 shortens the half-life of several miRNAs in d

25 c acids, triggering their destruction by Cas nucleases(2).

26 ropose the name Crn3 (CRISPR associated ring nuclease 3) for the Csx3 family.

27 A2 nuclease/helicase is a structure-specific nuclease, 5'-to-3' helicase, and DNA-dependent ATPase.

28 at employs coexpression of CRISPR-associated nucleases 9 and 12a (Cas9 and Cas12a) and machine-learni

29 tion of the mitochondrial genome by designer nucleases(9,10).Here we describe an interbacterial toxin

30 Combining nuclease accessibility and epigenetic states produced a

31 to active or inactive states, and shifts in nuclease accessibility of CTCF-bound elements.

32 eases after exposure to beta-lactams (termed nuclease-accessibility AST [nuc-aAST]).

33 The viral ring nuclease AcrIII-1 is widely distributed in archaeal and

34 question of how and when Mus81-Mms4 and Yen1 nucleases act on DNA replication or recombination struct

35 e potential for clinical implementation of a nuclease-activatable fluorescent probe for early diagnos

36 Thus, the nuclease-activatable P2&3TT probe distinguishes clinical

37 ems, where they likely function as accessory nucleases activated by cyclic oligoadenylate second mess

38 This mode of MutLgamma nuclease activation might explain crossover-specific pro

39 and the conformational changes required for nuclease activation.

40 et preserves the spatial organization of the nuclease active site, arguing that OLD proteins use a co

41 HEPN nuclease motifs create Las1's composite nuclease active site, but the roles of the individual HE

42 A long from the Chi recognition site to the nuclease active site.

43 A from both the Chi-recognition site and the nuclease active site.

44 Bax1 contains two distinguished nuclease active sites to presumably incise DNA damage.

45 i hosts, indicating that both the ATPase and nuclease activities are required for OLD function in viv

46 h HEPN nuclease motifs are required for Las1 nuclease activity and fidelity.

47 Additionally, nuclease activity at sites targeted by imperfectly match

48 f the reported methods enable control of the nuclease activity in bacteria.

49 ity in the Csx1 domain and a potent cA4 ring nuclease activity in the C-terminal Crn2 domain.

50 However, Pso2 lacks translesion nuclease activity in vitro, and mechanistic details of t

51 ain fold, and the mechanistic basis for ring nuclease activity is discussed.

52 L in vitro We noted that stimulation of Pso2 nuclease activity is specific to eukaryotic RecQ4 subfam

53 DNA by a mechanism that does not require the nuclease activity of Mre11.

54 gions distinct from those that stimulate the nuclease activity of MRN.

55 investigated each variant's effect on the 5' nuclease activity of PLD3, finding that some variants le

56 e structure to license a metal-dependent DNA nuclease activity specific for nicking of supercoiled DN

57 ISPR enzymes are RNA-targeting proteins with nuclease activity that enable specific and robust target

58 antly, Hrq1 also stimulated Pso2 translesion nuclease activity through a site-specific ICL in vitro W

59 e DNA repair function of EXO5 due to loss of nuclease activity, as well as failure of nuclear localiz

60 Despite the loss of nuclease activity, fluorescence binding assays confirm t

61 leading genome editing technologies rely on nuclease activity, including nick generation, at specifi

62 om viral proteases through gain of secondary nuclease activity.

63 an be unmasked by inactivating the intrinsic nuclease activity.

64 y sites in the genome but do not permit Cas9 nuclease activity.

65 fectors, and presence or absence of the ring nuclease activity.

66 efforts to achieve temporal control over its nuclease activity.

67 ates TerL ATPase activity, and inhibits TerL nuclease activity.

68 gnal transduction within the CRISPR-Cas9 HNH nuclease, advancing our understanding of the allosteric

69 that measures DNA accessibility to exogenous nucleases after exposure to beta-lactams (termed nucleas

70 esulted in PAM profiles distinct from either nuclease, allowing more flexible editing in human cells.

71 SpRY nuclease and base-editor variants can target almost all

72 side-chain methyl groups of the 64-kDa Mre11 nuclease and capping domains, which allowed us to descri

73 NA aptamers remain active in the presence of nuclease and exhibit markedly higher thermal stability t

74 he presence of Cas3, which contains both the nuclease and helicase activities required for DNA cleava

75 ns both single-stranded DNA (ssDNA)-specific nuclease and motor activities.

76 rom the region interfacing the adjacent RuvC nuclease and propagates up to the DNA recognition lobe i

77 s the Cas9 (CRISPR Associated protein 9) DNA nuclease and single guide RNA components, and difference

78 ents, but rather are induced from cell death nucleases and are not fundamental to the mechanism of ac

79 ite belongs to the 'PD-D/EXK' superfamily of nucleases and contains the motif SD-X11-EAK.

80 biochemical properties of DNA2-like helicase-nucleases and DNA looping motor proteins in general.

81 Here, we screened a panel of Cas9 nucleases and identified a small Cas9 ortholog from Stap

82 ope of biotechnological applications of Cas9 nucleases and may be particularly advantageous for genom

83 protected against detrimental activities of nucleases and of the DNA damage response machinery and p

84 be instrumental in understanding better both nucleases and their incompletely understood roles in vit

85 ses, transcriptional activator-like effector nucleases and, most recently, clustered regulatory inter

86 ants such as exoenzymes (proteases, lipases, nucleases) and downregulate the expression of surface bi

87 y mutagenic because it uses DNA polymerases, nucleases, and other enzymes that modify incompatible DN

88 ruses and bacteriophage encode a potent ring nuclease anti-CRISPR, AcrIII-1, to rapidly degrade cA4 a

89 However, in contrast to CRISPR-Cas9 nuclease approaches, the efficiency of CRISPRi/a depends

90 ctivities of enterobacterial RecBCD helicase-nuclease are coordinated by Chi recombination hotspots (

91 ion, and prokaryotic genomes that encode Ago nucleases are enriched in CRISPR-Cas systems.

92 CRISPR-Cas9 nucleases are powerful genome engineering tools, but unw

93 Type II CRISPR-Cas9 RNA-guided nucleases are widely used for genome engineering.

94 on components of MGEs, such as site-specific nucleases, are 'guns for hire' that can also function as

95 , as indicated by results of the micrococcal nuclease assay.

96 Our nuclease assays confirm this prediction and demonstrate

97 single-molecule FRET (smFRET)- and gel-based nuclease assays, we show that Hrq1 stimulates the Pso2 n

98 ort half-lives of such labile ligands due to nuclease attack and limited cellular uptake due to their

99 ll termini of the components are hidden from nuclease attack, whereas the target-binding sites are ex

100 of CRISPR-Cas-derived genome editing agents-nucleases, base editors, transposases/recombinases and p

101 Here we describe a PIK3CA mutation specific nuclease-based enrichment assay, which combined with a l

102 h ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that EZH1 could pa

103 time, recent demonstrations of programmable nuclease-based technology suggest that clinical manipula

104 om Sulfurisphaera tokodaii (St) bound to the nuclease Bax1 and their complex with a bubble DNA having

105 In archaea, XPB is associated with a nuclease Bax1.

106 resistance to degradation by four different nucleases, bovine and human serum, and human urine.

107 specialised for this task are known as ring nucleases, but are limited in their distribution.

108 nate the genome-wide activity of CRISPR-Cas9 nucleases, but are not easily scalable to the throughput

109 ritance of the X-chromosome-shredding I-PpoI nuclease by coupling this to a CRISPR-based gene drive i

110 her, our findings show that miniature Cas12f nucleases can protect against invading dsDNA like much l

111 hort Palindromic Repeats (CRISPR)-associated nuclease (Cas)-based sensing.

112 onsive materials using the CRISPR-associated nuclease Cas12a as a user-programmable sensor and materi

113 The CRISPR RNA (crRNA)-guided nuclease Cas13 recognizes complementary viral transcript

114 cleoprotein complex Cascade and the helicase-nuclease Cas3(4,5), but nuclease-deficient type I system

115 Here, we used the processive nuclease Cas3, together with a minimal Type I-C Cascade-

116 Using the RNA-guided nuclease Cas9, we induced two DNA double-strand breaks,

117 ers, protein nanocages, and the gene-editing nuclease Cas9, with up to 5-fold higher expression level

118 elied exclusively on the prototypical CRISPR nuclease Cas9.

119 the catalytic machinery controlling Class 2 nuclease cleavage, degenerate conservation of the C-term

120 Unprotected DNA is subjected to nuclease cleavage, resulting in replication catastrophe.

121 Our results demonstrate that the nuclease cofactor and structural functions of CtIP may d

122 ng mode reminiscent of that observed for the nuclease colicin E9.

123 from the 5' end enables the application of a nuclease competent Cas9 protein for transcriptional modu

124 ently recruited by gRNA aptamer binding to a nuclease competent CRISPR complex containing truncated g

125 Cas9 nucleases complexed with a guide RNA (Cas9-gRNA) find th

126 OLD family nucleases contain an N-terminal ATPase domain and a C-te

127 In this study, we used the RNA-guided nuclease CRISPR-Cas9 (clustered regularly-interspaced sh

128 ubsequently, we identified the cellular ring nuclease Crn1, which slowly degrades cA(4) to reset the

129 ribonuclease (Csx1) and a cA4-degrading ring nuclease (Crn2) from Marinitoga piezophila.

130 At Chi hotspots (5' GCTGGTGG 3'), RecB's nuclease cuts the 3'-ended strand and loads RecA strand-

131 Using the CRISPR/nuclease-deactivated Cas9 (dCas9)-based CARRY (CRISPR-as

132 prehensive map of the energetic landscape of nuclease-dead Cas12a (dCas12a) from Francisella novicida

133 deno-associated virus (AAV) vectors encoding nuclease-dead Cas9 and a single-guide RNA targeting CUG

134 n the less severe resection defects of MRE11 nuclease-deficient cells compared to those lacking CtIP.

135 ctasia-like disorder (ATLD) fibroblasts with nuclease-deficient MRE11A (p.W210C) tended to show slowe

136 ade and the helicase-nuclease Cas3(4,5), but nuclease-deficient type I systems lacking Cas3 have been

137 The potential of nuclease-deficient zinc fingers, TALEs or CRISPR fusion

138 emonstrated by reversible protection against nuclease degradation and trapping transient RNA complexe

139 regions of heterochromatin via resistance to nuclease degradation followed by next-generation sequenc

140 them into cancer cells and susceptibility to nuclease degradation.

141 chromosomal rearrangements characteristic of nuclease-dependent procedures.

142 Here we describe NucleaSeq-nuclease digestion and deep sequencing-a massively paral

143 Aptamers are often prone to nuclease digestion, which limits their utility in many b

144 acid (TNA) that is completely refractory to nuclease digestion.

145 res with dendritic oligonucleotides prevents nuclease digestion.

146 ques, including DNase-seq, which is based on nuclease DNase I, and ATAC-seq, which is based on transp

147 n of two regions of the Cas10 protein: an HD nuclease domain (which degrades viral DNA)(1,2) and a cy

148 in architecture consisting of a Cas3-like HD nuclease domain fused to a degenerate polymerase fold an

149 stems, however, the histidine-aspartate (HD) nuclease domain is encoded as part of a Cas10-like large

150 Interestingly, the DNA is kept away from the nuclease domain of Bax1, potentially preventing DNA inci

151 cture of the CTD indicates it is a vestigial nuclease domain that likely evolved from conserved nucle

152 position Bax1 at the forked DNA allowing the nuclease domain to incise one arm of the fork.

153 se domain that likely evolved from conserved nuclease domains in phage terminases.

154 opening and coordinating damage incision by nucleases during NER, but the underlying mechanisms rema

155 anded DNA breaks (DSBs) made by programmable nucleases (e.g. CRISPR-Cas9).

156 of human HSPCs as a feasible alternative to nuclease editing for HSC-targeted therapeutic genome mod

157 ng the G551D variant obtained by zinc finger nuclease editing of a human complementary DNA superexon,

158 n in erythroid progeny after base editing or nuclease editing was similar.

159 nfortunately, currently available small Cas9 nucleases either display low activity or require a long

160 In particular, two Cas12a nucleases encoded by Prevotella ihumii (PiCas12a) and Pr

161 ure shields phage DNA from CRISPR-associated nucleases encompassing Cascade-Cas3, Cas9, and Cas12.

162 nally, the MutLgamma-MutSgamma-EXO1-RFC-PCNA nuclease ensemble preferentially cleaves DNA with Hollid

163 igen (PCNA) are additional components of the nuclease ensemble, thereby triggering crossing-over.

164 e archaeal species encode a specialised ring nuclease enzyme (Crn1) to degrade cyclic tetra-adenylate

165 Structural comparisons of nuclease enzymes suggest that this Glu(Asp)-mediated mec

166 tion with AGO typically protects miRNAs from nucleases, extensive pairing to some unusual target RNAs

167 Enzymes of the 5' structure-specific nuclease family are crucial for DNA repair, replication,

168 nucleotide-binding (HEPN) domain-containing nuclease family.

169 indings suggest that high affinity of a Cas9 nuclease for its cognate PAM promotes higher genome-edit

170 he potential to be harnessed as programmable nucleases for genome editing.

171 ught to compare affinities of different Cas9 nucleases for their cognate PAM sequences.

172 nalyse the activity of a bacterial Argonaute nuclease from Clostridium butyricum (CbAgo) in vivo.

173 , we characterize CcCas9, a Type II-C CRISPR nuclease from Clostridium cellulolyticum H10.

174 the full-length structure of the Class 1 OLD nuclease from Thermus scotoductus (Ts) at 2.20 angstrom

175 To this end, we measured affinities of Cas9 nucleases from Streptococcus pyogenes, Staphylococcus au

176 Bacterial Cas9 nucleases from type II CRISPR-Cas antiviral defence syst

177 es of the Las1 HEPN motif were important for nuclease function, revealing that both HEPN motifs parti

178 the two HEPN domains is important for proper nuclease function.

179 he structural and dynamic landscape of Mre11 nuclease function.

180 Several families of ring nucleases functionally associated with sensor-only CARF

181 McrBC complexes are motor-driven nucleases functioning in bacterial self-defense by cleav

182 domains into those with both sensor and ring nuclease functions, and sensor-only ones.

183 cularization for high-throughput analysis of nuclease genome-wide effects by sequencing' (CHANGE-seq)

184 d in a tunnel in RecC but activates the RecB nuclease, > 25 A away.

185 NA cleavage sites and end trimming varied by nuclease, guide RNA and the positions of mispaired nucle

186 ay that depends on the MLH1-MLH3 (MutLgamma) nuclease has been implicated in the biased processing of

187 The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and c

188 ne systems in prokaryotes whose RNA-directed nucleases have been co-opted for various technologies.

189 The CRISPR/Cas9 nucleases have been widely applied for genome editing in

190 In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome editing field.

191 function at an ICL protects against DNA2-WRN nuclease-helicase complex and not the MRE11 nuclease tha

192 DNA2 is an essential nuclease-helicase implicated in DNA repair, lagging-stra

193 DNA2 nuclease/helicase is a structure-specific nuclease, 5'-t

194 The guanine-rich nuclease hypersensitivity element III(1) present in the

195 eful in exploring the activity of engineered nucleases in genome editing and other biotechnological a

196 Finally, P-AscH(-) decreased CTC-derived nucleases in subjects with stage IV PDAC in a phase I cl

197 tion CRISPR/Cas9 activation systems based on nuclease inactive dead (d)Cas9 fused to transcriptional

198 unhooking of the tethered strands by either nuclease incision of the DNA backbone or glycosylase cle

199 are resolved in opposite planes by targeting nuclease incisions to specific DNA strands(4).

200 iments on serum samples and experiments with nuclease indicated the contribution of encapsidated doub

201 res provide a platform to understand the XPB-nuclease interactions important for the coordination of

202 Inhibition of IRE1alpha nuclease interrupts the five components feedforward loop

203 Unlike DfCas9, the PpCas9 nuclease is active in human cells.

204 of genomic sequences using paired CRISPR-Cas nucleases is a powerful tool to study gene function, cre

205 off-target cleavage profile of programmable nucleases is an important consideration for any genome e

206 Genome editing using programmable nucleases is revolutionizing life science and medicine.

207 ats are susceptible to cleavage by the MUS81 nuclease, leading to massive chromosome shattering.

208 ated Rossman fold (CARF) domains and two DNA nuclease-like domains.

209 te of repair bubble extension by the XPB and nuclease machine.

210 High-resolution micrococcal nuclease mapping showed that ZL0580 induces a repressive

211 olysis activity is stimulated by its partner nuclease McrC.

212 e modification which enhances stability from nuclease mediated degradation.

213 rgeted Ubash3a in NOD mice using zinc-finger nuclease mediated mutagenesis.

214 enome that could be inadvertently altered by nuclease-mediated cleavage.

215 However, DNs are prone to nuclease-mediated degradation and are unstable in low Mg

216 utics show improved metabolic stability from nuclease-mediated degradation and exhibit enhanced inter

217 cellular genes counters the well-known viral-nuclease-mediated host shutoff and (ii) subsequent trans

218 counter to the well-known mechanism of viral-nuclease-mediated host shutoff that is activated downstr

219 We then turn to the recent surge of nuclease-mediated techniques and how they are changing t

220 tial sensitivity of chromatin to micrococcal nuclease (MNase) digestion, we profile accessible chroma

221 Here, using the micrococcal nuclease (MNase)-based chromatin accessibility (MACC) as

222 RNA cleavage pathways and share a short HEPN nuclease motif (RphiXXXH) important for RNA cleavage.

223 and in vitro assays, we show that both HEPN nuclease motifs are required for Las1 nuclease activity

224 Two juxtaposed HEPN nuclease motifs create Las1's composite nuclease active

225 by MRE11 homolog double-strand break repair nuclease (MRE11).

226 ndrial DNA due to the lack of the DNA repair nuclease MRE11A and inefficient lysosomal tethering of A

227 Bacterial RecBCD helicase-nuclease must coordinate DNA unwinding and cutting to re

228 trapped in NETs is facilitated by S. aureus nuclease (Nuc)-mediated degradation of NET DNA.

229 (HhH)(2) domain to couple with the XPF-ERCC1 nuclease/nuclease-like domains.

230 phage PhiKZ segregates its DNA from immunity nucleases of its host, Pseudomonas aeruginosa, by constr

231 ally, this signaling pathway includes a ring nuclease, often also a CARF domain (either the sensor it

232 ndirect cleavage by recruiting an endogenous nuclease, or a ribonuclease targeting chimera (RIBOTAC).

233 HEPN nucleases participate in diverse RNA cleavage pathways a

234 Most HEPN nucleases participate in stress-activated RNA cleavage p

235 ar necklace but also endows it with superior nuclease properties and antibacterial activities.

236 While these nucleases readily utilized each other's guide RNAs, they

237 The APEX2 gene encodes APE2, a nuclease related to APE1, the apurinic/apyrimidinic endo

238 Off-target editing by these nucleases remains a considerable concern, especially in

239 Following MRE11 nuclease removal of SPO11, the DSB is resected and loade

240 isingly, dose-dependent activity against the nuclease reporter (nuc), which is under the control of t

241 Gene editing nuclease represented by Cas9 efficiently generates DNA d

242 d with mobile genetic elements that lack the nucleases required for interference.

243 This small nuclease requires an 'NNNNRTT' PAM orthogonal to that of

244 arly steps of ICL repair to prevent aberrant nuclease resection, the role of BRCA2 in this process ha

245 cations and delivery strategies that improve nuclease resistance and enhance cell penetration.

246 gainst low-salt denaturation and to increase nuclease resistance by up to ~400-fold.

247 monstrates high affinity to RNA, exceptional nuclease resistance, efficient recruitment of RNase H, a

248 echnique that allows rapid identification of nuclease resistant chromatin, which correlate with heter

249 3'-extension by nick-translation to produce nuclease-resistant oligonucleotides and 3'-terminal tran

250 by the secreted S. aureus enzyme micrococcal nuclease results in emission of a readily detectable flu

251 1), CRISPR/Cas-derived RNA-guided engineered nuclease (RGEN), high resolution melt curve analysis (HR

252 e for flagellin and type I pili, but not the nuclease, S-layer protein, or serratamolide biosurfactan

253 Site-directed nucleases (SDNs) used for targeted genome editing are po

254 ters display increased H3K27 acetylation and nuclease sensitivity and coordinate induction of TNF, LT

255 ficant energetic stabilization and decreased nuclease sensitivity as unimolecular hairpin structures

256 The differential nuclease sensitivity assay accurately predicts previousl

257 Using a differential nuclease sensitivity assay, we investigate the chromatin

258 Nuclease sensitivity assays indicated that IRs are assoc

259 we evaluate the thermodynamic stability and nuclease sensitivity of oligonucleotides composed of the

260 s protocol describes single-cell micrococcal nuclease sequencing (scMNase-seq), a method for detectin

261 l-established biochemical theme, but how one nuclease site cleaves both DNA strands of a double helix

262 of genetic mutations, usually using targeted nucleases such as CRISPR/Cas9, and suppression of gene e

263 by binding and activating ancillary effector nucleases such as Csx1.

264 ne editing have been enabled by programmable nucleases such as transcription activator-like effector

265 editing technology is an emerging RNA-guided nuclease system initially identified from the microbial

266 uch as transcription activator-like effector nucleases (TALENs) and CRISPR-Cas9.

267 In particular, advances in sequence-specific nuclease technologies have dramatically accelerated the

268 s is requisite for activation of the Artemis nuclease that associates with DNA-PK to mediate hairpin

269 so it is highly likely that cells require a nuclease that can process remaining unresolved and hemi-

270 ody, and we show here that human ANKLE1 is a nuclease that cleaves a range of branched DNA species.

271 nuclease-helicase complex and not the MRE11 nuclease that is implicated in the resection of HU-induc

272 ifferentiation of PC12 cells by inhibiting a nuclease that promotes RNA-induced silencing (C3PO).

273 y described as an RNA deadenylase, is a ring nuclease that rapidly degrades cyclic tetra-adenylate (c

274 detecting foreign RNA, activating ancillary nucleases that can be toxic to cells, necessitating mech

275 nd messenger to signal infection, activating nucleases that degrade the nucleic acid of both invader

276 proteins (Acrs) to inactivate the RNA-guided nucleases that enforce CRISPR-Cas adaptive immunity in t

277 compact (422-603 amino acids) CRISPR-Cas12f nucleases that recognize and cleave dsDNA in a PAM depen

278 Cas12a nucleases therefore can exhibit widely varying propertie

279 ssays, we show that Hrq1 stimulates the Pso2 nuclease through a mechanism that requires Hrq1 catalyti

280 ages evade a broad spectrum of DNA-targeting nucleases through the assembly of a protein barrier arou

281 s showed that the HpSGN system required less nuclease to cleave ssDNA substrates than the SGN system

282 Werner (WRN) helicase, RPA directs the DNA2 nuclease to degrade the 5'-strand.

283 scribed into guide RNAs that direct the Cas9 nuclease to its target on the invader.

284 cterial CRISPR-Cas systems employ RNA-guided nucleases to destroy phage (viral) DNA.

285 (crRNAs) that guide CRISPR-associated (Cas) nucleases to destroy the invader's DNA or RNA.

286 ANCD2-FANCI heterodimer, which then recruits nucleases to remove the DNA lesion.

287 th meganucleases and followed by zinc finger nucleases, transcriptional activator-like effector nucle

288 om Streptococcus pyogenes was the first Cas9 nuclease used for genome editing and it remains the most

289 and Cas9 bacterial CRISPR RNA (crRNA)-guided nucleases used widely for genome editing and DNA detecti

290 munity by means of a potent anti-CRISPR ring nuclease variant AcrIII-1.

291 inactivating virions, as well as endogenous nucleases, was optimized to increase sensitivity and sam

292 Yet, only a few Type II-C nucleases were fully characterized to date.

293 3-19K lumenal domain activates the IRE1alpha nuclease, which initiates mRNA splicing of X-box binding

294 tly repaired by a pathway involving the Pso2 nuclease, which is hypothesized to use its exonuclease a

295 Unlike inhibitors of DNA-cleaving Cas nucleases, which cause limited immunosuppression and req

296 endonuclease 1 (FEN1), a structure-specific nuclease with roles in DNA replication and repair, and h

297 rothermophilus Bad, a bacterial DNA helicase-nuclease with similarity to human DNA2.

298 ber of known CRISPR-Cas subtypes to identify nucleases with novel properties.

299 s that results from its cleavage by targeted nucleases, with broad implications for the study and pot

300 However, the functional diversity of nucleases within each subtype remains poorly explored.