ライフサイエンスコーパス: nucleoporin

1 th (PCD) in plants, is a novel transmembrane nucleoporin.

2 regulation via the phosphorylation status of nucleoporins.

3 ular condensates containing distinct sets of nucleoporins.

4 ng that the CTD does not interact with other nucleoporins.

5 partners for the O-GlcNAc-modified FG-repeat nucleoporins.

6 ir cargoes, regulatory factors, and specific nucleoporins.

7 iple copies of ~30 different proteins called nucleoporins.

8 with nuclear import and export receptors and nucleoporins.

9 atomic-resolution crystal structures of most nucleoporins.

10 phobic phenylalanine-glycine (FG) domains on nucleoporins.

11 ELYS and the nucleoporin 107-160 complex, components of mitotic kinet

12 ssive missense mutation in the gene encoding nucleoporin-107 (NUP107) that results in abnormal ovaria

13 identified a recessive missense mutation in nucleoporin-107 (NUP107, c.1339G>A, p.D447N).

14 beta transportin-3 (TNPO3) and NPC component nucleoporin 153 (NUP153) as being important for infectio

15 Here, we report that Nucleoporin 153 (NUP153) interacts with the chromatin ar

16 IV(smPBj1.9) physically interacts with human nucleoporin 153 (Nup153), which is known to provide a do

17 olyadenylation specific factor 6 (CPSF6) and nucleoporin 153 kDa (NUP153), bind to the CA hexamer wit

18 Nucleoporins 153 and 358, which bind HIV-1 capsid, play

19 g revealed that EDS4 encodes the ortholog of NUCLEOPORIN 205, a core component of the inner ring of t

20 cific activation of the testis-specific gene nucleoporin 210 like (NUP210L) in brain in some individu

21 es revealed that nucleoporin p62 (NUP62) and nucleoporin 214 (NUP214) are differentially distributed

22 found that the nuclear pore protein Nup214 (nucleoporin 214) and its interaction partner Nup88 negat

23 import, including transportin 3 (TNPO3) and nucleoporin 358 (NUP358), in the targeting of gene-dense

24 e nuclear pore complex via interactions with nucleoporins(4,5).

25 Here, we show that nucleoporin 62 (Nup62) is a chromatin-bound protein and

26 Nucleoporin 62 (Nup62) was found to bind to nsp1beta, an

27 nsp1beta binds to the cellular protein nucleoporin 62 (Nup62), and as a consequence, the nuclea

28 hanges in importin-beta1, nucleoporin 98 and nucleoporin 62 nuclear rim staining are observed in Purk

29 Knockdown of nucleoporin 62 strongly inhibited viral morphogenesis, w

30 mRNA export factor 1 (Rae1) and nucleoporin 98 (Nup98) are host cell targets for the mat

31 Fusion proteins involving Nucleoporin 98 (NUP98) are recurrently found in acute my

32 bip1 (bric a brac interacting protein 1) and Nucleoporin 98 (Nup98) as additional regulators of the e

33 Nucleoporin 98 (NUP98) fusion oncoproteins are observed

34 Analogous changes in importin-beta1, nucleoporin 98 and nucleoporin 62 nuclear rim staining a

35 emia (AML), a genetic fusion protein between nucleoporin 98 and the third plant homeodomain (PHD) fin

36 The nucleoporin 98 gene (NUP98) is fused to a variety of par

37 Structural chromosomal rearrangements of the Nucleoporin 98 gene (NUP98), primarily balanced transloc

38 encodes an Arabidopsis homolog of mammalian nucleoporin 98, a component of the nuclear pore complex

39 complicates investigations of how individual nucleoporins act in these diverse processes.

40 Recently we determined that the nucleoporin ALADIN participates in spindle assembly in s

41 Our data demonstrate a novel function of the nucleoporin Alm1 in proteasome localization required for

42 nsists of as many as 224 copies of the three nucleoporins, amounting to a molar mass of 12.3 MDa and

43 ese studies reveal that knockdown of certain nucleoporins and components of nucleocytoplasmic traffic

44 uit CRM1, export cargo proteins, and certain nucleoporins and concomitantly affect nuclear protein an

45 ew our current knowledge of the structure of nucleoporins and how those come together in situ.

46 and, in addition, is enriched for subsets of nucleoporins and inner nuclear membrane (INM) proteins,

47 ins that participate in mRNA export, such as nucleoporins and mRNA export adaptors, were mislocalized

48 xperiments on the functional requirements of nucleoporins and nuclear lamins.

49 We observed crosslinking between FG-repeat nucleoporins and nuclear transport factors, suggesting t

50 res of the interacting domains between these nucleoporins and pieced together the molecular architect

51 nt, preventing the inheritance of long-lived nucleoporins and senescence factors into the newly forme

52 r envelope herniations (blebs) containing FG-nucleoporins and ubiquitin are the phenotypic hallmark o

53 NUP98, a nucleoporin, and its interaction partner Rae1, have been

54 sts cell-type-specific functions for certain nucleoporins, and gene expression profiling has revealed

55 g isoforms, posttranslational modifications, nucleoporins, and higher-order oligomerization of nucleo

56 built from multiple copies of ~30 different nucleoporins, and understanding how these nucleoporins a

57 and Kalverda et al. now reveal that certain nucleoporins are actively involved in transcription insi

58 opy, we have explored the mechanism by which nucleoporins are altered in nuclei isolated from C9orf72

59 t the nanoscale organization and function of nucleoporins are context dependent in a way that is requ

60 Nucleoporins are essential components of the nuclear por

61 Nucleoporins are linked with cell-type-specific gene reg

62 se 1 (SENP1) and SENP2, we observe that many nucleoporins are mislocalized and, in some cases, reduce

63 e complex is disassembled and, increasingly, nucleoporins are proving to have mitotic functions when

64 eolus, containing ribosomal DNA repeats, the nucleoporins are required for aggregation of heterochrom

65 We further show several nucleoporins are required for the complete assembly of R

66 es and support the proposal that the adaptor nucleoporins arose from ancestral karyopherins.

67 dependent transcription complex and define a nucleoporin as a key player in the CNS.

68 Using nucleoporins as a paradigm for long-term protein persist

69 nt nucleoporins, and understanding how these nucleoporins assemble into the NPC scaffold imposes a fo

70 In the prepore model, nucleoporins assemble on the chromatin as an intermediat

71 bout 30 different protein components, termed nucleoporins, assemble in multiple copies into an intric

72 We show that the spatial organization of FG nucleoporin assemblies with the transport proteins can b

73 We found that nucleoporin-associated SEs localize preferentially to th

74 duced gene expression patterns and chromatin-nucleoporin association, suggesting that Nup155-mediated

75 gene expression through changes in chromatin-nucleoporin association.

76 A subset of nucleoporins bear a domain with multiple phenylalanine-g

77 Overexpression of importin-beta, or of the nucleoporin-binding region, inhibited RANGAP1 recruitmen

78 The central region (harboring nucleoporin-binding sites) regulates microtubule dynamic

79 Strikingly, we found that nucleoporins can be released from nuclear bodies and rei

80 the growing body of evidence that structural nucleoporins can have novel tissue-specific roles.

81 (Exportin-1), MEX67/MTR2 (TAP/p15), and five nucleoporins cause accumulation of unspliced tRNA, a hal

82 ture of a reconstituted ~400-kilodalton coat nucleoporin complex (CNC) from Saccharomyces cerevisiae

83 e that the structured domains in the adaptor nucleoporin complex are held together by peptide interac

84 which is a TREX-2 homolog that is part of a nucleoporin complex, functions as part of a cryptic aspe

85 he interactions between the approximately 30 nucleoporins comprising the modular structure of the nuc

86 better understand how a family of "adaptor" nucleoporins--concentrically surrounding this channel--m

87 ec13, Nup75, Nup93, and Nup205, are scaffold nucleoporins considered important for the overall integr

88 ough the nuclear pore complex (NPC) requires nucleoporins containing natively unfolded phenylalanine-

89 Nucleoporins containing phenylalanine glycine (FG) repea

90 The RanBP2 nucleoporin contains an internal repeat domain (IR1-M-IR

91 Notably, each of the three nucleoporins contains additional nuclear pore complex bi

92 vity associated with a kinetochore-localized nucleoporin contributes to two key events that occur dur

93 provides mechanistic insights into how these nucleoporins coordinate nucleocytoplasmic transport to m

94 rred to as cytoplasmic assemblies of PML and nucleoporins (CyPNs), after cell division.

95 P107 in ovarian development and suggest that nucleoporin defects may play a role in milder and more c

96 Nucleoporin depletions suggest that translocation throug

97 The cohesive nature of a human nucleoporin did not necessarily correlate with that of i

98 Here, we demonstrate specific defects in nucleoporin distribution in strains lacking Heh1p and He

99 a tethered layer of intrinsically disordered nucleoporin domains containing Phe-Gly (FG)-rich repeats

100 molecular assembly of intrinsically unfolded nucleoporin domains rich in phenylalanine-glycine dipept

101 It consists of nucleoporin domains rich in phenylalanine-glycine motifs

102 ween Saccharomyces cerevisiae Nup159 and the nucleoporin, Dyn2.

103 These "channel" nucleoporins each contain an ordered region of approxima

104 ndidates with high confidence, including the nucleoporin ELYS, lamin B1, and four proteins (emerin, M

105 trictly dependent on POM121, a transmembrane nucleoporin essential for interphase nuclear pore biogen

106 Of the 23 nucleoporins evaluated, we observed a reduction in a sub

107 In particular, Nup159, a nucleoporin exclusively located on the cytoplasmic side

108 e and neuronal differentiation, but how this nucleoporin exerts its function and whether it modulates

109 N-terminal half of Nup98, which contains the nucleoporin FG/GLFG repeat motifs.

110 Intrinsically disordered Phe-Gly nucleoporins (FG Nups) within nuclear pore complexes exe

111 enylalanine-glycine (FG) motifs, known as FG nucleoporins (FG nups), that play the key role in the NP

112 ffinity of Kapbeta1 to phenylalanine-glycine nucleoporins (FG Nups), which is comparable with RanGTP.

113 surface is composed of phenylalanine-glycine nucleoporins (FG Nups)--intrinsically disordered protein

114 from a family of phenylalanine-glycine-rich nucleoporins (FG-Nups) to control nucleocytoplasmic tran

115 hed in disordered phenylalanine/glycine-rich nucleoporins (FG-Nups), which form a permeability barrie

116 inding disordered phenylalanine-glycine-rich nucleoporins (FG-Nups).

117 s and also substituted FG domains from other nucleoporins for the Nup98 domain to directly compare co

118 sis by locally removing NPP-3 and associated nucleoporins from the NE.

119 d their role at nuclear pore complexes, some nucleoporins function in the nucleoplasm.

120 unexpected mechanistic complexities based on nucleoporin functions and specialized import and export

121 The nucleoporin gene NUP98 is fused to several genes includi

122 gous nonsense or splice-site variants in the nucleoporin genes NUP37, NUP43, and NUP188, which have n

123 tants was suppressed by deletion of specific nucleoporin genes.

124 Previously, we identified the nucleoporin gp210/Nup210 as a critical regulator of musc

125 They also indicate that nucleoporins have an active role in plant signaling.

126 Nucleoporins have been reported to regulate pluripotent

127 The Nsp1*Nup49*Nup57 channel nucleoporin heterotrimer (CNT) attaches to the IRC solel

128 a new role for the pore-associated SENPs in nucleoporin homeostasis and in achieving proper configur

129 h Nup98a/b, two phenylalanine-glycine repeat nucleoporins implicated in maintaining the selective nuc

130 er of the NPC and supporting a role for this nucleoporin in the permeability barrier.

131 ted proteins and suggest a role for specific nucleoporins in heterochromatin function.

132 ogenesis, is underrepresented relative to FG-nucleoporins in nuclear envelopes of Torsin-deficient ce

133 te vacuole-dependent degradation of specific nucleoporins in nup116DeltaGLFG cells.

134 ks between HEH1 and HEH2, and genes encoding nucleoporins in the membrane, inner, and outer ring comp

135 We characterize the behavior of the FG nucleoporins in vivo using polarized fluorescence micros

136 Surprisingly, many of these nucleoporins, including Sec13, Nup75, Nup93, and Nup205,

137 various conformational transitions of the FG nucleoporins induced by the cargo-carrying transport pro

138 core a yet-uncharacterized function of these nucleoporins inside the nucleus, even in cells that cont

139 on, suggesting that Nup192 possesses another nucleoporin interaction partner.

140 he importance of finely adjusted karyopherin-nucleoporin interactions for efficient cargo translocati

141 ecture and the molecular details of the coat nucleoporin interactions forming the central "triskelion

142 Thus, TorA-nucleoporin interactions might be abrogated by TorA-Delt

143 NPC and validating their placement with our nucleoporin interactome, we built a composite structure

144 here it acts with Cdk1 to hyperphosphorylate nucleoporin interfaces to promote NPC disassembly and nu

145 P-5 is dispensable for incorporation of most nucleoporins into nuclear pores and for nuclear protein

146 his maintenance is limited, however, as some nucleoporin levels decrease during aging, providing a ra

147 itiation of a pathological cascade affecting nucleoporin levels within neuronal nuclei and ultimately

148 sically disordered polypeptides, known as FG nucleoporins, lining its passageway.

149 These effects on nucleoporin localization are likely of functional import

150 or Nup358, the two major Phe-Gly (FG) repeat nucleoporins localized on the cytoplasmic side of the NP

151 actions between the structured core scaffold nucleoporins, mediate the assembly of the inner ring com

152 velope, to uncover an intriguing role of the nucleoporin MEL-28 in mediating chromosome segregation v

153 how that the conserved kinetochore-localized nucleoporin MEL-28/ELYS docks the catalytic subunit of p

154 NE lumen is sufficient to suppress both the nucleoporin mislocalization and growth defects in heh1De

155 This is the first evidence for a nucleoporin modulating the import reaction by directly a

156 re reproducibly enriched with AAL, including nucleoporins, mRNA-processing enzymes, and cell-signalin

157 he defect in the yeast temperature-sensitive nucleoporin mutant nup159.

158 Finally, we used different anti-nucleoporin nanobodies to purify the major NPC building

159 taches to the IRC solely through the adaptor nucleoporin Nic96.

160 rest is suppressed in mutant embryos lacking nucleoporin NPP-16/NUP50 function, indicating that this

161 terest, this screen revealed that the Nup205 nucleoporin NPP-3 can negatively modulate the timing of

162 We identify the central channel nucleoporins NPP-1/Nup58, NPP-4/Nup54, and NPP-11/Nup62

163 The ceNXF2 NTF2 domain bears at least two nucleoporin (Nup) binding pockets necessary for the colo

164 idely expressed variant of the transmembrane nucleoporin (Nup) Pom121 (named sPom121, for "soluble Po

165 , we provide evidence that the transmembrane nucleoporin (Nup), POM121, but not the Nup107-160 comple

166 ase and two Cdk phosphorylation sites in the nucleoporin Nup1 were required for peripheral targeting

167 ed to the NPC since a fusion of Mex67 to the nucleoporin Nup116 rescues a deletion of MEX67 Thus, Mex

168 promoted by the interaction of Drg1 with the nucleoporin Nup116.

169 Here we show that the nucleoporin Nup153 interacts with Sox2 in adult NeuPCs,

170 We find the dynamics of nucleoporin Nup153 to be regulated so as to produce rapi

171 th its CID and MCM3AP domains, together with nucleoporin Nup153.

172 Although SNX9 does not bind nucleoporins Nup153 or Nup214 or some beta importins (Im

173 (AID) system to distinguish roles of basket nucleoporins NUP153, NUP50 and TPR.

174 ermined the crystal structure of one adaptor nucleoporin, Nup157.

175 lel IDP duplexes with some partners, such as nucleoporin Nup159 and dynein intermediate chain, the mo

176 r pore assembly through its interaction with nucleoporin Nup159.

177 PCs) during interphase is facilitated by the nucleoporin Nup2 via its importin alpha- and Ran-binding

178 ated by the histone deacetylase Sir2 and the nucleoporin Nup2.

179 The transmembrane nucleoporin Nup210 is absent in proliferating myoblasts

180 thogenic variants in the gene encoding human nucleoporin NUP214 causing acute febrile encephalopathy.

181 proteins interacted with CRM1, Ran, and the nucleoporin NUP214 in a manner fundamentally different f

182 LNO1, a homolog of the nucleoporin NUP214 in human (Homo sapiens) and Nup159 in

183 indicate that a 137-amino-acid region of the nucleoporin Nup214 is a binding site for the major AdV c

184 nism requires the phenylalanine-glycine (FG)-nucleoporin Nup35, which is consistent with use of the n

185 characterized the species-specific scaffold nucleoporin Nup37 and ELY5/ELYS.

186 tion proceeds through the acquisition of the nucleoporin Nup37.

187 Finally, we showed that mutation of the nucleoporin Nup50 increased the lifespan of TDP-43 trans

188 work, we have studied the interaction of the nucleoporin Nup50 with importin alpha5.

189 d domain in interaction with another channel nucleoporin (Nup54) and a transport factor (Kapbeta1).

190 We recently showed that the three "channel" nucleoporins, Nup54, Nup58, and Nup62, interact with eac

191 nsport, which is thought to consist of three nucleoporins, Nup54, Nup58, and Nup62.

192 ing between a structured domain of a channel nucleoporin (Nup58) and its neighboring disordered domai

193 ires the DNA sequence of the contacted gene, nucleoporins Nup60 and Nup2, and cohesin.

194 We find that the nucleoporin Nup62 and the C termini of the nephronophthi

195 , finding that ICP27 directly binds the core nucleoporin Nup62.

196 Three out of approximately 30 nucleoporins, Nup62, Nup54, and Nup58, line the nuclear

197 Here we show that the conserved nucleoporin Nup93 is essential for NPC assembly and conn

198 l lines in which we endogenously labeled the nucleoporin Nup96 with mEGFP, SNAP-tag, HaloTag or the p

199 teraction between HCV capsid protein and the nucleoporin Nup98 at cytosolic lipid droplets that is im

200 ists of an N-terminal FG-rich portion of the nucleoporin NUP98 fused to the homeodomain region of the

201 ecule mRNA analyses, we demonstrate that the nucleoporin Nup98 is required for full expression of p21

202 In this issue, Singer et al. reveal that the nucleoporin Nup98 supports adaptation to genotoxic stres

203 We find a contribution of the nucleoporin Nup98 to mitotic spindle assembly through re

204 One such nucleoporin, Nup98, binds chromatin and regulates gene e

205 Nucleoporins (Nups) are a family of proteins best known

206 Nucleoporins (NUPs) are an essential component of the nu

207 Nucleoporins (Nups) are involved in neural development,

208 Recent studies have shown that a subset of nucleoporins (Nups) can detach from the nuclear pore com

209 addition to mediating transport, a subset of nucleoporins (Nups) engage in transcriptional activation

210 However, how nucleoporins (Nups) exert this control remains poorly un

211 Intrinsically disordered nucleoporins (Nups) form a selective filter inside the N

212 tive studies have unveiled a full catalog of nucleoporins (Nups) that comprise the NPC, structural ar

213 NPCs lacking these nucleoporins (Nups) were blocked from entry into the dau

214 In ooc-5-mutant germ cell nuclei, nucleoporins (Nups) were mislocalized in large plaques b

215 conformational changes to select domains of nucleoporins (Nups) within the inner ring (Nup54, Nup58,

216 Of approximately 30 nucleoporins (Nups), 15 are structured and form the Y an

217 embled from approximately 30 proteins termed nucleoporins (Nups), mediates selective nucleocytoplasmi

218 They are formed by about 30 nucleoporins (Nups), which can be roughly categorized in

219 ose of nuclear transport and is dependent on nucleoporins (NUPs), which comprise a ciliary pore compl

220 f approximately 30 different proteins called nucleoporins (Nups).

221 tterns of individual NPC components known as nucleoporins (Nups).

222 by nuclear pore complexes (NPCs) made up of nucleoporins (NUPs).

223 led from multiple copies of approximately 30 nucleoporins (Nups).

224 tures composed of approximately 30 proteins (nucleoporins [Nups]).

225 About 30 different proteins (nucleoporins, nups) arrange around a central eightfold r

226 The cytoplasmic filament nucleoporins of the nuclear pore complex (NPC) are criti

227 nanobodies that recognize seven constituent nucleoporins of the Y and Nic96 complexes.

228 fragments of three cytoplasmically oriented nucleoporins of yeast: Nup82, Nup116, and Nup159.

229 perphosphorylation of nuclear pore proteins (nucleoporins or Nups), including Nup62, Nup153, and Nup2

230 one another and a subset of NPC components (nucleoporins or Nups).

231 assembly of ~500 individual proteins, called nucleoporins or nups.

232 m approximately 30 different proteins called nucleoporins or Nups.

233 ear envelope and composed of proteins termed nucleoporins (or "Nups"), and (2) nuclear transport fact

234 i from orthogonal perspectives revealed that nucleoporin p62 (NUP62) and nucleoporin 214 (NUP214) are

235 Herein, we show that partial knockdown of nucleoporin p62 (NUP62) by small-interfering RNA confers

236 gene expression profiling has revealed that nucleoporin p62 (NUP62) transcripts are decreased in the

237 ith dysplasia, but the immunoreactivities of nucleoporin p62, DEP-domain containing protein 5, clathr

238 DRA2, together with other nucleoporins, participates positively in the control of

239 We conclude that nucleoporins play an unanticipated regulatory role in NE

240 NPP-16/NUP50 function, indicating that this nucleoporin plays an important role in prophase arrest i

241 pore complex scaffold and the transmembrane nucleoporin POM121.

242 cadherin-like lumenal domain of the membrane nucleoporin Pom152p.

243 yeast (Saccharomyces cerevisiae), encodes a nucleoporin protein containing phenylalanine-glycine rep

244 Nucleoporin proteins (Nups) have been proposed to mediat

245 ular mRNA translation and cleavage of select nucleoporin proteins (Nups) within nuclear pore complexe

246 including the cleavage of Phe/Gly-containing nucleoporin proteins (Nups) within nuclear pore complexe

247 ring position of the unfolded domains of the nucleoporin proteins (the FG-Nups), which control select

248 lone, several regulatory factors (e.g., RNA, nucleoporin proteins, and the endogenous small molecule

249 raction of RanGAP1 stably interacts with the nucleoporin RanBP2 at a binding site that is flanked by

250 We show an unexpected function of a nucleoporin, RanBP2, in maintaining BA homoeostasis thro

251 n insights into possible mechanisms by which nucleoporins regulate pluripotency in a pro-arrhythmogen

252 crystallographic analysis of these scaffold nucleoporins revealed the molecular details of their int

253 a novel interacting partner of the conserved nucleoporin Seh1 and add to the growing body of evidence

254 e show that tissue-specific depletion of the nucleoporin Seh1 causes dramatic myelination defects in

255 ssue-specific requirement for the structural nucleoporin Seh1 during Drosophila oogenesis.

256 (2019) in this issue of Neuron uncovers that nucleoporin Seh1 is required for the expression of genes

257 FG domains from several human nucleoporins showed no interactions in this assay; howev

258 Nup192 is a major component of an adaptor nucleoporin subcomplex proposed to link the NPC coat wit

259 nt mediates interactions with the Nup107-160 nucleoporin subcomplex.

260 oporins, and higher-order oligomerization of nucleoporin subcomplexes.

261 PC) during interphase and to require certain nucleoporins, such as Tpr in animal cells, to properly l

262 Using nucleoporins tagged with green fluorescent protein along

263 the sole E2 for SUMOylation, and of TPR, the nucleoporin that forms the basket on the nuclear side of

264 Nup214/CAN, a nucleoporin that is found at the cytoplasmic side of the

265 known interaction between Nek2 and Nup98, a nucleoporin that localizes to the ciliary base, is impor

266 Nup358 is a metazoan-specific nucleoporin that localizes to the cytoplasmic filaments

267 omal region is the gene coding for Nup214, a nucleoporin that localizes to the cytoplasmic side of th

268 NUP98 is a nucleoporin that plays complex roles in the nucleocytopl

269 Nup153 is the nucleoporin that provides this scaffold for Nup50.

270 enylalanine-glycine (GLFG) repeat-containing nucleoporin that, in addition to nuclear transport, cont

271 Furthermore, we discovered that NPP-3 and nucleoporins that are associated with it are lost from t

272 re related and evolutionary conserved, large nucleoporins that are part of the NPC scaffold.

273 rties of intrinsically disordered regions of nucleoporins that bind transport factors.

274 e complexes (NPCs) is densely filled with FG-nucleoporins that form a permeability barrier of a still

275 Recent evidence indicates that structural nucleoporins, the building blocks of the NPC, have a var

276 ls that NS1 prevents binding of NXF1-NXT1 to nucleoporins, thereby inhibiting mRNA export through the

277 association of identified target genes with nucleoporins through interaction with Nup155.

278 s indirect tethering to FG-repeat-containing nucleoporins through karyopherins.

279 lates the affinity of the import complex for nucleoporins, thus dictating its persistence inside the

280 Here we show that the addition of the Nup210 nucleoporin to NPCs during myoblast differentiation resu

281 eractome, allowing assignment of trypanosome nucleoporins to discrete NPC substructures.

282 and the NTF2-like and UBA domains binding FG-nucleoporins to facilitate movement through nuclear pore

283 complex to the region that interacts with FG-nucleoporins to facilitate passage through nuclear pores

284 Cleaved p75(NTR) interacts with nucleoporins to promote Smad2 nucleocytoplasmic shuttlin

285 ed by cross-linking highly O-GlcNAc-modified nucleoporins to proteins involved in nuclear transport.

286 g is required to increase the trafficking of nucleoporins to the cell cortex in stressed or meiotical

287 es of such long-lived proteins, histones and nucleoporins, to understand the function of protein long

288 s broad applicability by analyzing images of nucleoporins, TORC1 complexes, endocytic vesicles and Ba

289 In particular, the nucleoporin TPR is necessary for both formation and main

290 describe the link between the nuclear basket nucleoporins (Tpr and Nup153) and chromatin organization

291 blies formed from approximately 30 different nucleoporins, typically organized in subcomplexes.

292 crease in the expression of seven additional nucleoporins, ultimately affecting the localization of R

293 Using recombinant nucleoporins, we show that Sp-Nup37 specifically binds t

294 Ubiquitin and nucleoporins were identified as distinctively localizing

295 Interactions with specific nucleoporins were pinpointed using FLIM-FRET for measuri

296 s has particular relevance for the FG domain nucleoporins, which are crucial for nucleocytoplasmic tr

297 approximately 30 different proteins, termed nucleoporins, which assemble to form one of the largest

298 import, resembling importin interaction with nucleoporins, which, in turn, promote complex disassembl

299 e by regulating the distribution of specific nucleoporins within the nuclear pores.

300 egments ("protomers") of the three "channel" nucleoporins yielded a model for how this channel is con