ライフサイエンスコーパス: operon

1 units in a single vector (~10.6-kb synthetic operon).

2 ntaining a low basal expression of the hdrRM operon.

3 ase-dependent derepression of the mexAB-oprM operon.

4 ene transcription, including that of the dev operon.

5 TA-3'], which exists upstream of the PfmqsRA operon.

6 in the opposite direction to the rest of the operon.

7 ionary history of the phenazine biosynthetic operon.

8 f the cytochrome bd oxidase-encoding cydABDC operon.

9 und to be a direct activator of the ddl ddlR operon.

10 m of diauxie led to the discovery of the lac operon.

11 ional intermediate forms leading to the full operon.

12 ntergenic region upstream of the ycgZ-ymgABC operon.

13 5 biofilm formation mediated by the PFLU1114 operon.

14 chia coli harboring the plasmid-borne mccB17 operon.

15 members of the capsular polysaccharide (cps) operon.

16 d C71), FisR activates the expression of the operon.

17 to orthologs of the mmpS5-mmpL5 drug efflux operon.

18 ch encodes only three unique subunits in its operon.

19 of yxaJ, the yybNM operon and the sunA-bdbB operon.

20 xpressed the five enzymes from the P. putida operon.

21 nly one, OppA, is encoded in the E. coli opp operon.

22 to unusual aspects of regulation of the lac operon.

23 previously shown for tlrB and the trpEDCFBA operon.

24 wo Cys residues, to derepress the mexAB-oprM operon.

25 yptophan-dependent attenuation of the trpRBA operon.

26 This activity is specified by the mer operon.

27 ries different regulatory sequences for this operon.

28 transcriptional repressor of the mexAB-oprM operon.

29 hastic behaviors in transcription of the lac operon.

30 t regulates methionine synthesis via the met operon.

31 tors and DNA looping that are shared by many operons.

32 onors involving both single genes and entire operons.

33 identifying possible intermediate states of operons.

34 ons located downstream of highly transcribed operons.

35 han 25 genes, most of which are encoded in 3 operons.

36 nd archaeal genomes are often organized into operons.

37 um tuberculosis have over 90 toxin-antitoxin operons.

38 hes a role for 3'-UTRs as evolved eukaryotic operons.

39 within two different antibiotic biosynthesis operons.

40 egulates the E. coli K-12 and Salmonella nrf operons.

41 those typically seen in toxin-antitoxin (TA) operons.

42 nce of four putative plasmids harbouring rib operons.

43 preferential evolutionary emergence of miRNA operons.

44 sentation of the genomic contexts of Acr-Aca operons.

45 n localized between the citAB and citCDEFXGT operons.

46 conditions were profiled by sequencing rRNA operons.

47 the human CF operon CS18 and the porcine CF operon 987P (F6).

48 nts, we show that genes arranged in a single operon, a cascade, or as two operons all give rise to th

49 by the activity of glycosyltransferase (gtr) operons acquired by horizontal gene transfer.

50 ged in a single operon, a cascade, or as two operons all give rise to the different protein compositi

51 ets the nirC cistron of the long nirBDC-cysG operon, an observation that we rationalize as a mechanis

52 the constitutive transcription of the entire operon and a second promoter (P (mer) ), located within

53 promoter of the nopaline oxidase gene (nox) operon and activate the transcription of noxB-lacZY, res

54 promoter of the octopine oxidase gene (oox) operon and activate the transcription of ooxB-lacZY, res

55 operon structure that we named noncontiguous operon and consists in an operon containing a gene(s) th

56 iption at the P2 and P3 promoters of the agr operon and increase biofilm formation, and two of these

57 acterized locus scfCDE was transcribed as an operon and is predicted to encode an ABC importer for nu

58 a the bioH gene is within a biotin synthesis operon and its transcription is coregulated with the oth

59 ene is not located within a biotin synthetic operon and its transcription is not coregulated with the

60 deletion of the endogenous riboflavin (rib) operon and presence of four putative plasmids harbouring

61 leading to increased expression of the hsmRA operon and subsequent deployment of HsmA to capture heme

62 to repress transcription of yxaJ, the yybNM operon and the sunA-bdbB operon.

63 rgenic region separating the capsule ctr-css operons and adjacent deletion of cssA/B/C and a part of

64 In bacteria, operons and gene blocks provide a model of tractable evo

65 The genes in operons and gene blocks typically work together in the s

66 n between the number of cytochrome c oxidase operons and heterotrophic or diazotrophic capabilities.

67 ive bacterial exopolysaccharide biosynthetic operons and in other fungal genomes.

68 onale for genomic retention of certain miRNA operons and may explain preferential evolutionary emerge

69 e dichotomy of a prokaryotic gene structure (operons and polarity) and eukaryotic molecular homology

70 ecific to Europe consistently lacks motility operons and that it is immotile in vitro, probably due t

71 genome regulation, excluding the presence of operons and trans-splicing.

72 Mn (mtsABC) and Fe acquisition systems (sia operon), and a metal-independent DNA synthesis enzyme (n

73 egulation by creating a synthetic endogenous operon, and identified an enhancer region that contains

74 from substrates and is encoded in a two-gene operon, and the other protein, CidA, binds CidB.

75 yrimidine biosynthesis operon, the betA-betB operon, and the pathogenicity island, SaPI5, while virul

76 tes of the ribosome on leadered transcripts, operons, and on leaderless transcripts, the latter repre

77 suboptimal canonical miRNAs are enriched in operons, and we validate several of these experimentally

78 encoded in widespread conserved bicistronic operon architectures that are reminiscent of those typic

79 s protection mechanisms present on the nrfAH operon are also found in the P. gingivalis genome, we sh

80 Operons are a hallmark of bacterial genomes, where they

81 At the molecular level, operons are classic examples of a complex system.

82 Interestingly, DAB operons are found in a diverse range of bacteria and arc

83 co-located genes with related functions, and operons are gene blocks whose genes are co-transcribed o

84 However, the composition and architecture of operons are highly variable and frequently differ even a

85 Although hipBA operons are widespread in bacterial genomes, it is unkno

86 ucture should be revisited by including this operon arrangement in which cotranscription and overlapp

87 on (LOF) mutations in the efflux pump mtrCDE operon as a mechanism of increased antibiotic susceptibi

88 n average of 60.7 +/- 19.1 and 36.5 +/- 10.6 operon associated genes respectively.

89 expression, expanding the repertoire for trp operon attenuation beyond TrpL- and TRAP-mediated mechan

90 up to 60 parts (or mixtures); monocistronic, operon-based or hybrid configurations; and a new streaml

91 ence homology and coexpression from the same operon, both proteins differ in their membrane localizat

92 erichia coli, which has an endogenous arsRBC operon but lacks an arsH ortholog.

93 Rho promotes termination at the end of operons, but it can also terminate transcription within

94 t to the concept of post-transcriptional RNA operons, but we further present evidence that nuclear pr

95 of loop extrusion, long, highly transcribed operons can significantly impede the extrusion process.

96 terize three proteins encoded by a conserved operon carried by the Staphylococcal Cassette Chromosome

97 The deletion of genes from the wca operon caused a persistent-infection E. coli strain to b

98 ducing homogenous plant Rubisco by rbcL-rbcS operon chloroplast transformation.

99 was therefore proposed that the related cin operon codes for an alternative CI system.

100 Two proteins encoded by the comF operon, ComFA and ComFC, are involved in transformation

101 mplate to amplify and assemble an artificial operon composed of four gene clusters encoding 13 pilus

102 re also found to be the main inducers of the operon, confirming that the encoded proteins are involve

103 Bacterial mRNAs are organized into operons consisting of discrete open reading frames (ORFs

104 amed noncontiguous operon and consists in an operon containing a gene(s) that is transcribed in the o

105 rdinated expression of genes in several long operons, contributing to the key physiological effects o

106 Presence of the functional M6 operon correlated with an enhanced ability of clinical s

107 y regulates the staphyloxanthin-biosynthetic operon crtOPQMN, promoting survival of S. aureus in the

108 egulator of the staphyloxanthin-biosynthetic operon, crtOPQMN, but is epistatic to alternative sigma

109 seven genes, csmA-G, related to the human CF operon CS18 and the porcine CF operon 987P (F6).

110 riking finding was high induction of the CS5 operon (csfA-F), its putative transcription factor csvR,

111 Two of these operons (DAB1 and DAB2) contain a two-gene locus that en

112 Second, trp operon de-repression is profoundly deleterious when infe

113 Bacterial genes are typically grouped into operons defined as clusters of adjacent genes encoding f

114 genes (Deltaexlx and Deltagh5) and the full operon (Deltaexlx-gh5) resulted in decreased severity of

115 The crtOPQMN operon driving pigment synthesis is under the control of

116 While colocalization within a bacterial operon enables coexpression of the constituent genes, th

117 We hypothesized that the operon-encoded BioH might differ in its expression level

118 In bacteria, the kdp operon encodes a four-subunit potassium pump that mainta

119 Here we show that the Wolbachia cin operon encodes a nuclease, CinB, and a second protein, C

120 The Escherichia coli K-12 nrf operon encodes a periplasmic nitrite reductase, the expr

121 The mqsRA operon encodes a toxin-antitoxin pair that was character

122 Here, we report that overexpression of an operon encoding a highly conserved inorganic phosphate i

123 ssion of multiple genes including the emhABC operon encoding a major multidrug efflux pump.

124 tridium peptide RumC depends on an R. gnavus operon encoding five potential RumC precursor peptides a

125 g the T7 bacteriophages engineered with lacZ operon encoding for beta-galactosidase (beta-gal).

126 Functional enrichment analyses identify an operon encoding oxaloacetate decarboxylase as diagnostic

127 C-peps induced the expression of the scaCBA operon encoding the lipoprotein adhesin ScaA, which was

128 found within the oxygen-regulated pufQBALMX operon encoding the reaction centre-light-harvesting pho

129 vieA is part of an operon encoding the VieSAB signal transduction pathway t

130 of the genomic region encompassing the parEC operon encoding topoisomerase IV.

131 presented uPSS and iPSS in the polycistronic operons encoding ribosomal proteins, and the majority up

132 The Wolbachia cid operon, encoding 2 proteins, CidA and CidB, the latter a

133 essing from the 3'UTR of the sdhCDAB-sucABCD operon, encoding enzymes of the tricarboxylic acid (TCA)

134 and IS1655 element movement in the pilMNOPQ operon, encoding the type IV pilin biogenesis apparatus.

135 ranscription of lapA and the lapBC, and lapE operons, encoding a LapA-specific secretion system.

136 f pufQ and the photosystem genes in the same operon ensures that switching of tetrapyrrole metabolism

137 those interested in exploring gene block and operon evolution.

138 The operon exerts its effect via an encoded transcription fa

139 toxin rather than in the antitoxin controls operon expression and toxin activity.

140 he sigma factor HasI and upregulation of has operon expression.

141 ess conditions demand the discoordination of operon expression.

142 mportant member of the network is the master operon, flhDC, which encodes the FlhD4C2 protein.

143 t of an unannotated gene in the biosynthetic operon for NOS, as an acyl carrier protein that delivers

144 The bicistronic genBA operon (formerly named celBA) of the opportunistic patho

145 his operon, which we name the flow-regulated operon (fro), we establish that P. aeruginosa dynamicall

146 Deletion of genes in the wca operon from a persistent-infection strain resulted in a

147 l reporter strains possessing a modified lux operon from Photorhabdus luminescens.

148 In this study, we used the bza operon from the anaerobic bacterium Moorella thermoaceti

149 f the Proteus vulgaris higBA toxin-antitoxin operon from the Rts1 plasmid.

150 involves five reactions catalyzed by the bza operon gene products, namely the hydroxybenzimidazole sy

151 ription is coregulated with the other biotin operon genes.

152 all remodeling genes (lytN, ddh), the urease operon, genes involved in metal transport (feoA, mntH, s

153 ns, including osteomyelitis, and the msaABCR operon has been implicated as an important factor in mod

154 scribed spacer 1 (ITS1) and ITS2 of the rRNA operon has played an important role in understanding the

155 Here, we show that this operon has two convergently expressed and differentially

156 Many of the genes in the Mbn operons have been assigned functions, but two genes usua

157 the most variable rRNAs, encoded by the rrnI operon (herein designated as I-ribosomes), direct the pr

158 r recycling in translational coupling within operons; if a ribosome remains bound to an mRNA after te

159 rver specifically mining genomes for Acr-Aca operons; (ii) it provides a most comprehensive Acr and A

160 We found that a deletion in the p27-p55 operon impairs the replication of M. bovis in bovine mac

161 everal quinones as potent inducers of the M6 operon in addition to triclosan.

162 ch aptamer domain (GSR(apt)) of the xpt-pbuX operon in Bacillus subtilis Unlike what had been observe

163 ransporter from a lanthipeptide biosynthetic operon in Lachnospiraceae and demonstrate that this prot

164 The lar operon in Lactobacillus plantarum encodes five Lar prote

165 n important function to the MmpS6-MmpL6 (M6) operon in Mtb stress management.

166 Controlled expressions of this operon in nonpathogenic E. coli strains led to efficient

167 al of Mtb, signifying the importance of this operon in oxidative stress survival in mycobacteria.

168 ungi and reflects the polycistronic arginine operon in prokaryotes.

169 Expression of the cin operon in transgenic male flies induces male sterility a

170 e encoded by different numbers of homologous operons in Actinobacteria.

171 e now known, and expression of reconstructed operons in heterologous hosts has confirmed that most pa

172 ectly, the expression of dozens of genes and operons in Listeria monocytogenes.

173 revealing the use of functional bicistronic operons in mammals.

174 ranslation of long virulence and conjugation operons in many Gram-negative bacterial pathogens.

175 highlight the central role of toxin-antidote operons in Wolbachia-induced cytoplasmic incompatibility

176 ession of the formate hydrogenlyase 1 (fhl1) operon increased with added H2 .

177 ne protein system (HsmRA) and show that this operon induces a protective response that repurposes hem

178 duced singly and in combination with the lux operon into a methicillin-resistant S. aureus strain.

179 enotype reverted upon reintroduction of this operon into the mutant.

180 tR, which directly regulates the pga and fap operons involved in the synthesis of extracellular polym

181 A paralogous operon involves a putative nuclease (CinB) rather than a

182 The Escherichia coli marRAB operon is a paradigm for chromosomally encoded antibioti

183 The pso operon is conserved among rickettsial pathogens, suggest

184 The Pseudomonas virulence factor (pvf) operon is essential for the biosynthesis of two very dif

185 The pelABCDEFG operon is highly conserved among diverse bacterial speci

186 The zauPzapA operon is present in diverse Gram-negative bacteria, ind

187 Expression of the genBA operon is regulated by the transcriptional activator Gen

188 iously demonstrated that the HH103 rkpLMNOPQ operon is responsible for 5-acetamido-3,5,7,9-tetradeoxy

189 gel-shift experiments demonstrated that the operon is under negative control of the global carbon ca

190 arrangement of functionally-related genes in operons is a fundamental element of how genetic informat

191 ng Wolbachia strains lack a DUB-encoding cid operon; it was therefore proposed that the related cin o

192 s were found to be the potassium transporter operon kdp and an ABC transporter operon of uncharacteri

193 The operon, known to influence the formation of curli fibres

194 Inactivation of genes within the arn operon led to increased susceptibility of P. aeruginosa

195 ual leaderless short ORFs confer independent operon-level control, while their shared dependence on c

196 o identify which of these are encoded within operon-like structures that are part of the accessory ge

197 an adjacent putative restriction enzyme (RE) operon likely form a restriction modification (RM) syste

198 ted regions were in the 5'-ends of genes and operons mainly containing tRNA genes and a few protein-c

199 how a simple vertical evolution model of how operons may evolve from the individual component genes.

200 This revealed that the arn operon, mediating arabinosaminylation of lipopolysacchar

201 ts, indicating that dysregulation of the arn operon might explain increased AZM uptake and synergy in

202 -affinity phosphate transport (pstSCAB-phoU) operon: no increase in polyphosphate kinase 1 (ppk1) tra

203 n, we demonstrated that induction of the lac operon occurs in only a fraction of the population, with

204 l deletions within the anthrose biosynthetic operon of B. anthracis strains from Chile and Poland.

205 ese indole derivatives de-repress the trpRBA operon of C. trachomatis strains with trpA or trpB mutat

206 The trp operon of Chlamydia trachomatis is organized differently

207 The tnaC regulatory gene from the tna operon of Escherichia coli controls the transcription of

208 The lac operon of Escherichia coli is a model system with regula

209 ually deleting each of the genes of the mce4 operon of M. smegmatis, which mediates the transport of

210 ify the NAD(+) salvage pathway encoded by an operon of nadR-pnuC-nrtR (from SSU05_1973 to SSU05_1971

211 criptomic approach enabled us to discover an operon of P. aeruginosa that is rapidly and robustly upr

212 It resembles the cel operon of Streptococcus pyogenes, which is implicated in

213 The mer operon of the deeply branching thermophile Thermus therm

214 A regulator gene is adjacent to the operon of the sulfide-oxidizing genes, encoding a sigma(

215 ransporter operon kdp and an ABC transporter operon of uncharacterized function.

216 The two As resistance arsRBC operons of Pseudomonas putida KT2440 are followed by a d

217 rategy was validated by assembling synthetic operons of various sizes and determining the efficiency

218 Either deletion of the entire mce4 operon or single point mutations that eliminate the tran

219 le biochemical pathways, as well as multiple operons or regulons.

220 r cells can form in the absence of all hipBA operons or spoT, indicating that multiple pathways lead

221 on of uptake enzymes and requires a specific operon organization.

222 ToxR requires its operon partner ToxS, a periplasmic integral membrane pro

223 tiated by another enzyme encoded in the same operon, Ppt hydrolase (PptH), that undoes the PptT react

224 iments show that SAM-I(Xcc) controls the met operon primarily at the translational level in response

225 re, we investigate the expression of the nrf operon promoter from various pathogenic enteric bacteria

226 we examined the organization of the fimbrial operon promoters from other important EAEC strains to un

227 aracterized, and the precise role of the bza operon protein CobT is unclear.

228 Mutations in the polysaccharide synthesis operon (pso) abolish lipopolysaccharide O-antigen synthe

229 enting an early step in the evolution of mer-operon regulation.

230 n and the possibility to organize genes into operons represent considerable advantages that make chlo

231 nces virulence only in the context of purine operon repressor gene (purR) inactivation.

232 Recently, the ribose operon repressor, RbsR, was also defined as a pleiotropi

233 ding adenylsuccinate synthetase, and the cps operon required for capsule production.

234 ransposon insertion screen, we identified an operon required for glucosinolate metabolism in B. theta

235 ed region (3' UTR) of the oppABCDF and carAB operons, respectively, and base-pair with their own tran

236 Phylogenetic analysis of the operon revealed that Kpi belongs to the little-character

237 Our exploration of several candidate operons reveals that our new method provides more optima

238 er to a polycistronic mRNA molecule, and the operon's gene products often form molecular complexes or

239 An operon's genes are co-transcribed under the control of a

240 acter was obtained by PCR detecting atl, ica operon, sasG and agr.

241 tion on experimentally characterized Acr-Aca operons showed that AcrFinder had a 100% recall.

242 nent of the putative OG1RF_10750-OG1RF_10755 operon situated elsewhere on the chromosome to form a fu

243 parsimony algorithm to reconstruct ancestral operon states, and show a simple vertical evolution mode

244 Antarctic phylotype exclusively contains an operon structure consisting of a dicitrate transporter f

245 light of our results, the canonical view of operon structure should be revisited by including this o

246 istence of a higher level of organization in operon structure that we named noncontiguous operon and

247 We use DGCR8 tethering assays and operon structure-function assays to demonstrate that loc

248 e one observed in a mutant lacking all 6 mce operons suggesting a pleiotropic role for this system.

249 ted from studies of the Escherichia coli lac operon switch, in which DNA looping plays an essential r

250 tis and found that its gene is present in an operon that also contains genes predicted to encode a pu

251 ve genes, ssl2245 and sll1130, constitute an operon that also has characteristics of a toxin-antitoxi

252 is autoregulated and is typically part of an operon that contains other ars genes involved in arsenic

253 manLMN, was expressed as a mannose-inducible operon that exhibited the most influence on PTS sugar me

254 We identify an auxin-degradation operon that is conserved in all available genomes of Var

255 ted strains with Tn insertions in the mntABC operon that permitted growth in the presence of Cu.

256 gether regulate the transcription of the cus operon that plays important roles in cells' resistance t

257 or of the pyrimidine nucleotide biosynthetic operon that promoted abscess formation and colonization,

258 ffering potato (Solanum tuberosum) rbcL-rbcS operons that either encoded one of three mesophyll small

259 Here we investigate the role of two gtr operons that we identified in the S Typhi genome.

260 e urease operon, the pyrimidine biosynthesis operon, the betA-betB operon, and the pathogenicity isla

261 ble to rewire gene expression of the capsule operon, the main pneumococcal virulence factor, to be ex

262 lines expressing a codon optimized Rs-rbcLS operon, the messenger RNA (mRNA) abundance was ~25% of r

263 , highly upregulated loci include the urease operon, the pyrimidine biosynthesis operon, the betA-bet

264 se data suggest that, in addition to the arn operon, there are multiple factors in host-like environm

265 Encoded in operons, these components are likely representative of t

266 a coli controls the transcription of its own operon through an attenuation mechanism relying on the a

267 his vitamin distinctively regulates the cobA operon through its 5' untranslated region (5' UTR).

268 esults in downregulation of the entire T3SS1 operon through repression of its positive regulator exsC

269 AIPs) and the accessory gene regulator (agr) operon to coordinate expression of virulence factors req

270 by causing the repressor-bound states of the operon to equilibrate rapidly, thus ensuring that the su

271 bed spacer region 2 of the nuclear ribosomal operon to identify the fungal species present.

272 sing of RNase P-dependent polycistronic tRNA operons to release pre-tRNAs is the essential function o

273 The adaptation of the newly acquired operons to the eukaryotic setting involved a repertoire

274 2) gene, followed by rapid processing of the operon transcript into two monocistronic mRNAs.

275 due to steric clashes, hence derepresses TA operon transcription.

276 particular, a Y585C mutation in the sorbitol operon transcriptional regulator gutR was associated wit

277 identified a horizontal operon transfer (HOT) event, with clear evidence for sel

278 Kominek et al. identified a horizontal operon transfer (HOT) event, with clear evidence for sel

279 Here, we report the horizontal operon transfer of a siderophore biosynthesis pathway fr

280 Profiling of rRNA operons using the Oxford MinION yielded 65,706 2-D seque

281 Transcription of the eut operon was detected in the majority of urine samples con

282 Furthermore, transcription of the licT-blgPB operon was found to be repressed by glucose and induced

283 hich is located proximally to the archaellum operon was identified.

284 Transcription of the TTT-usp (4207) operon was induced in the presence of citrate and tartra

285 erse genomic context, it is likely that this operon was relocated several times during Pseudomonas ev

286 the medium, introduction of the complete M6 operon was sufficient to increase tolerance of the M6- s

287 The expression of citC and citS operons was dependent on citrate and CitAB, whose transc

288 ly by using individual genes within the same operon, we propose that the modulation of functional amy

289 carbohydrate when strains with an intact lac operon were also present in the cultures, indicative of

290 and disease states, revealing specific genes/operons whereby Spn adapts to and influences host sites

291 . fischeri-specifically, deletion of the lux operon, which abrogates symbiont luminescence-reduced th

292 ' untranslated region (UTR) of the rplM-rpsI operon, which encodes the ribosomal proteins (r-proteins

293 Using a single-cell reporter of this operon, which we name the flow-regulated operon (fro), w

294 All sequenced UPEC strains had conserved eut operons, while metabolic genotypes previously associated

295 E. tracheiphila has horizontally acquired an operon with a microbial expansin (exlx) gene adjacent to

296 In 5448, bglP was shown to be in an operon with a putative phospho-beta-glucosidase (bglB) d

297 Darobactin is coded by a silent operon with little production under laboratory condition

298 ow that crhR is expressed from a dicistronic operon with the methylthiotransferase rimO/miaB (slr0082

299 wed consistent expression of six hydrogenase operons with and without added H2 .

300 evealed a second conserved target, the ergAB operon, with functions unrelated to virulence.

301 n, we observed that disruption of a two-gene operon, yjbIH, resulted in decreased levels of pigmentat