ライフサイエンスコーパス: organ culture

1 lbar and forniceal conjunctiva were grown in organ culture.

2 eas for research and 3 days after storage in organ culture.

3 neas to determine their epithelialization in organ culture.

4 or segments (n = 7) were placed in perfusion organ culture.

5 S1P-induced decrease of outflow facility in organ culture.

6 nt tissues fails in vivo and, as we show, in organ culture.

7 EG mRNAs were strongly induced in human skin organ culture.

8 fit of mechanical stress to outflow cells in organ culture.

9 h undergoes involution, events replicated in organ culture.

10 was evaluated in the human ocular perfusion organ culture.

11 expressing Delta-like 1, or in fetal thymus organ culture.

12 elease from the chondrocytes was verified by organ culture.

13 respiratory tract using ex vivo respiratory organ culture.

14 ic development in CD3gamma(-/-) fetal thymic organ culture.

15 were appraised in perfused anterior segment organ culture.

16 as pathogenic as viruses from progressors in organ culture.

17 s using atherosclerotic coronary arteries in organ culture.

18 nd controls were exposed to GH in short-term organ culture.

19 sement membrane separation of oral mucosa in organ culture.

20 erosclerotic coronary arteries maintained in organ culture.

21 mize the growth factor or hormone milieu for organ culture.

22 in vitro in uremic rat parathyroid glands in organ culture.

23 lls and decreased neointimal lesions in lung organ culture.

24 y monitor cellular behavior in ex vivo whole organ culture.

25 al and temporal cellular behavior in ex vivo organ culture.

26 liary zonules in Ltbp2(-/-) mouse eyes under organ culture.

27 ited molecular responses both in vivo and in organ culture.

28 s, we analyzed microvasculature fragments in organ culture.

29 al influenza A viruses in primary human lung organ cultures.

30 lt chicken kidney cells and ex vivo tracheal organ cultures.

31 elerates viral gene expression in ganglionic organ cultures.

32 th mesenchymal stem cell lines and calvarial organ cultures.

33 ects of ROCK inhibition on mouse SMG ex vivo organ cultures.

34 uremic rats, as well as in mouse parathyroid organ cultures.

35 mmunodeficient mice, and murine fetal thymus organ cultures.

36 hanism according to in vitro seminal vesicle organ cultures.

37 lofenac enhanced collagen production in skin organ cultures.

38 ng retinal neurons in three-dimensional (3D) organ cultures.

39 submandibular salivary glands using ex vivo organ cultures.

40 C) was applied to anterior segment perfusion organ cultures.

41 inhibited branching morphogenesis in kidney organ cultures.

42 eal endothelial cells did not proliferate in organ culture after cryo-injury.

43 n reactivation of latent virus in ganglionic organ cultures all genes are derepressed at once, thus o

44 ion with neutralizing antibody in pancreatic organ culture also led to an increase in the number of e

45 In ex vivo kidney organ culture, an antagonist of Hnf4a (but not a similar

46 , increased hair synthesis in scalp follicle organ culture and advanced mouse pelage hair regrowth in

47 nd Xenopus embryonic development, mouse lung organ culture and an in vivo lung metastasis model.

48 dies on tetracyclines (e.g., doxycycline) in organ culture and animal models of bone-deficiency disea

49 Using reaggregated thymic organ culture and bone marrow chimeras, we demonstrate t

50 We designed two approaches using incisor organ culture and bromodeoxyuridine (BrdU) pulse-chase e

51 ar outflow pathway in human anterior segment organ culture and decrease IOP in Brown Norway rat eyes.

52 Organ culture and embryo analyses point to a loss of epi

53 We established a mouse embryonic heart organ culture and gene expression system that facilitate

54 d healing were determined in porcine corneal organ culture and HCEC scratch wound models.

55 ized in isolated feline iris preparations in organ culture and heterologously expressed G-protein-cou

56 on progenitor (TNP) marker scleraxis both in organ culture and in cultured cells, and disruption of T

57 In vitro organ culture and in vivo transplantation experiments in

58 anching morphogenesis of BMP4-exposed UGS in organ culture and show that the effects of BMP4 and NOGG

59 is with Double Markers (MADM), combined with organ culture and time-lapse imaging, to trace the movem

60 nts from human eyes were placed in perfusion organ culture and treated with the K(ATP) channel opener

61 Fourteen-day organ culture and Western blotting of iliac arteries and

62 diabetes (5 with diabetic retinopathy) were organ cultured and transduced with rAV-expressing c-met

63 l cells in chimeric human-mouse fetal thymic organ cultures and also in the presence of SCF and IL-15

64 ytogenes infection in primary human decidual organ cultures and in the murine decidua in vivo A high

65 tor-induced microvessel sprouting in ex vivo organ cultures and in vivo Matrigel plugs.

66 Notably, fetal thymic organ cultures and self-peptide administration showed in

67 kage, growth arrest, and apoptosis in keloid organ cultures and substantially inhibited angiogenesis.

68 racellular growth in primary human placental organ cultures and trophoblasts.

69 tissues were analyzed by histology, explant organ culture, and cell culture.

70 old storaged donor tissue and </=35 days for organ culture, and preservation time after split prior t

71 A/DNP increased exosome secretion from mouse organ cultures, and in vivo injections enhanced the leve

72 p-regulated Timp1 expression in the cell and organ cultures, and inhibition of aggrecan release by rh

73 Using loss-of-function assays in organ cultures, and targeted mesothelial-restricted hedg

74 TF3 is induced nearly 100-fold in ganglionic organ cultures; and (iv) ATF3 plays a key role in the ma

75 ) and did not develop subsequent to birth in organ culture, as in wildtype and heterozygous muscles.

76 g, could exert anti-KD effects in a novel KD organ culture assay and in keloid fibroblasts (KF).

77 event their epithelial differentiation in an organ culture assay.

78 Genetic studies and in vitro organ culture assays further demonstrate a role for Ror2

79 ntibiotic treatment, K8(-/-) colonocytes and organ cultures become less resistant to apoptosis and re

80 sue may be stored safely for up to 1 week in organ culture before use in DALK and DMEK surgery.

81 GFP+ cells into embryonic kidney explants in organ culture, beta-galactosidase immunohistochemistry s

82 /-) mice and a new method to silence LARG in organ-cultured blood vessels, we show that both RhoGEFs

83 In organ culture, BMP-2 substantially decreased relaxation

84 and matrix degradation in the parietal bone organ cultures by increasing differentiation and formati

85 ere modulated in bovine endometrial cell and organ cultures by small molecules that target the mevalo

86 Immunolabeling after 4-5 days in organ culture caused loss of large CGRP+ axons, but not

87 MMP-1 treatment of young skin in organ culture causes fragmentation of collagen fibrils a

88 urbing secretion in male embryonic gonads in organ culture causes male-to-female germ cell sex revers

89 yos showed less regression with added MIS in organ culture compared with control MDs when analyzed by

90 ey were then examined in reaggregated thymic organ cultures containing mixtures of monoclonal and pol

91 of miR-10b increased Ki-67 staining in human organ-cultured corneas and proliferation rate in culture

92 cence imaging of genetically marked cells in organ-cultured corneas and via computational modeling.

93 created by air injection into the stroma of organ-cultured corneas before fixation.

94 MiR-10b transfected human organ-cultured corneas showed downregulation of PAX6 and

95 s (rAV)-driven c-met overexpression in human organ-cultured corneas was tested for correction of diab

96 leral buttons from human donors (n = 19) and organ-cultured corneoscleral buttons (n = 10) obtained a

97 ial responses of HUAECs and HUVECs to TNF in organ culture correlate with transcription factor/co-act

98 in early thymic subpopulations, fetal thymic organ cultures cultured in the presence/absence of a p38

99 In vitro collagen gel assays and ex vivo organ culture data further confirmed that infection with

100 Inhibition of heparanase activity in organ culture decreased branching morphogenesis, and thi

101 he parasympathetic ganglion in mouse explant organ culture decreased the number and morphogenesis of

102 o assays by analyzing interactions in kidney organ cultures deficient in Oat1 and Oat3.

103 nt protein (GFP) injected into mice and skin organ culture delivered GFP to the cell surface of kerat

104 er short-term culture (Optisol-GS) at 4 C or organ culture (Dulbecco's modified Eagle's medium, Corne

105 Wnt5a(-/-) murine prostates rescued by organ culture exhibit disturbances in bud position and d

106 In tongue organ cultures, exogenous Wnt5a profoundly suppresses pa

107 Foreskin organ culture experiments confirmed our in vitro cell re

108 In vitro organ culture experiments demonstrated that miR-275 is i

109 Organ culture experiments further suggest that FGF signa

110 Using organ culture experiments we show that TCDD also repress

111 However, the utility of organ culture for studying HRV-C biology is limited.

112 reatment and reduced the healing time in eye organ culture from 30 to 20 hours.

113 secretions, (b) detected in media of vaginal organ culture from both Fbln5(-/-) and wild type mice, a

114 o and further confirmed using human arterial organ cultures from CKD patients, in vivo.

115 bnormal femoral growth plate and calvaria in organ cultures from embryos of the Fgfr3Y367C/+ mouse mo

116 and hypoxia (Sugen/hypoxia) as well as lung organ cultures from patients with pulmonary hypertension

117 Skin organ cultures from Tcrd-/-, which lack DETC, and Il17a-

118 NKT cells fail to develop in fetal thymic organ culture (FTOC) treated with MTP antagonists.

119 Thymic selection in fetal thymic organ cultures (FTOCs) allowed negative selection and ge

120 , inducing HSV gene expression in ganglionic organ cultures harboring latent virus and incubated in m

121 Metanephric organ culture has been used to determine whether embryon

122 trabecular meshwork (TM) cells in tissue and organ culture have been shown to contain cross-linked ac

123 Hair follicle organ cultures have previously been used to investigate

124 CD4(+) thymocytes in the human fetal-thymus organ culture (HF-TOC).

125 Here, we show that these organ-cultured HFs respond to a key cyclophosphamide met

126 significant induction in human fetal thymus organ culture (HFTOC).

127 Namely, 24 h organ-cultured hHFs treated with PGD2 displayed reduced

128 ere isolated from systemic contributions via organ culture, highlighted the innate tendon environment

129 d not spontaneously reactivate in ganglionic organ cultures; however, viral genes were expressed if t

130 Organ cultured human corneoscleral buttons were studied.

131 In this issue, Samuelov et al. transfected organ-cultured human hair follicles with siRNA nucleotid

132 sence of central clock influences, isolated, organ-cultured human HFs show circadian changes in the g

133 Finally, in organ-cultured human scalp hair follicles as well as in

134 ction in human epidermis, we investigated in organ-cultured human scalp HFs whether TRH (30 nM), TSH

135 cts of HJMD in vitro by transfecting normal, organ-cultured human scalp HFs with lipofectamine and CD

136 rial function in human epidermis, we treated organ-cultured human skin, or isolated cultured human ep

137 inase inhibitor 2A (p16(ink4)) expression in organ-cultured human skin.

138 re, we have explored whether microdissected, organ-cultured, human scalp hair follicles (HFs) in anag

139 In fetal thymus organ cultures, impeded Notch1 activation resulted in B

140 ured by epithelial removal and maintained in organ culture in the presence of EGF or LXA4 with or wit

141 marker genes of Kolliker's organ in cochlear organ cultures in a dose-dependent manner.

142 Fetal thymus organ cultures in JAG2-deficient thymic lobes or with No

143 We also reported that in the ganglionic organ cultures incubated in medium containing antibody t

144 DEX-treated cells and perfused organ cultures infected with AdhGRE.MMP1 secreted high l

145 D to cultured human sebocytes and human skin organ culture inhibited the lipogenic actions of various

146 immortalized cell lines, and currently sinus organ culture is the only system described that is permi

147 dissected human scalp HFs can be observed in organ culture, it permits the study of the unknown contr

148 herence to the human colon by using in vitro organ culture (IVOC) of colonic biopsy samples and polar

149 g Mullerian duct in rat urogenital ridges in organ culture manipulated by microincision and/or chemic

150 r, a WNT5A inhibitory antibody, added to UGS organ culture media, rescued prostatic budding from inhi

151 discs and placed in a well plate containing organ culture medium 1 without dextran.

152 donor corneas, 5 pairs each, were stored in organ culture medium before deswelling either at 31 C or

153 dissociated from human corneas and grown in organ culture medium.

154 In human intestinal organ cultures, microbial activation of Vgamma9/Vdelta2

155 The current serum-free nasal polyp organ culture model allows physiologically and clinicall

156 l wound healing was evaluated by a whole-eye organ culture model and by scratch-induced wound closure

157 We developed an air-liquid canine corneal organ culture model and evaluated its susceptibility to

158 stablishment of an air-liquid canine corneal organ culture model as a useful model to study ocular he

159 ing with DKK1 decreased cyst formation in an organ culture model of ADPKD.

160 inflammatory mucosal response in an ex-vivo organ culture model of PI-IBS D.

161 size of preformed cysts in a neonatal kidney organ culture model of polycystic kidney disease.

162 with epithelial differentiation utilizing an organ culture model of rat metanephric mesenchymal diffe

163 Finally, we used an ex vivo organ culture model to assess the effects of MyD88 inhib

164 , we utilize an established ex vivo mandible organ culture model to model these complex interactions.

165 We then used a human skin organ culture model to test the direct effects of these

166 dge of corneal epithelium after injury in an organ culture model, and that this change occurs despite

167 l re-epithelialization was assessed using an organ culture model, in which initial resurfacing result

168 Furthermore, in an organ culture model, TGFalpha can increase levels of pho

169 e to hypertonicity was studied in an ex vivo organ culture model, using wild-type and haploinsufficie

170 Using a tibia organ culture model, we confirmed that silencing Foxo1 d

171 ed using a porcine anterior segment-perfused organ culture model.

172 ithelial debridement using a mouse whole-eye organ culture model.

173 tivation and promote wound healing in a skin organ culture model.

174 lung branching morphogenesis in a fetal lung organ culture model.

175 ed using a porcine anterior segment-perfused organ culture model.

176 n human lymphoid tissue, we used a tonsillar organ culture model.

177 sured in a porcine anterior-segment-perfused organ culture model.

178 tory/immune-response in PI-IBS in an ex-vivo organ culture model.

179 f sphingosine-1-phosphate (S1P) in whole eye organ culture models decreases outflow facility, whereas

180 tilized our unique live mouse calvarial bone organ culture models under conditions which dissociates

181 In organ culture models, epithelium was removed and corneas

182 nels are present in human follicles, we used organ culture, molecular biological, and immunohistologi

183 In organ cultures, Monlis PT digest induced a significant i

184 In the organ-cultured murine lens, EP ameliorates oxidative str

185 Using organ-cultured nasal polyps as a surrogate tissue for hu

186 In cultured chondrocytes and murine limb organ culture, NF449 rescued FGFR3-mediated extracellula

187 ity, compared to conventional 4-week passive organ culture (OC), of an active storage machine (ASM) t

188 mma) and production of IFN-gamma/IL-15 after organ culture of celiac duodenal biopsy samples.

189 In organ culture of DR3-deficient mouse kidneys, addition o

190 an embryonic kidney cyst model involving 4-d organ culture of embryonic day 13.5 mouse kidneys in 8-B

191 of animals with Kit neutralizing antibody or organ culture of gastrointestinal tissues in the presenc

192 we propagated two HRV-C isolates ex vivo in organ culture of nasal epithelial cells, sequenced a new

193 clinical samples and function in short-term organ cultures of ccRCC tissue treated with wild-type TN

194 Ex vivo organ cultures of endometrium, endometrial cells and per

195 Organ cultures of gastric glands from wild-type or IL-1R

196 Organ cultures of human choroid were incubated in C5a or

197 Addition of TL1A to organ cultures of human or mouse kidney caused activatio

198 atRA synthesis was measured in organ cultures of isolated choroids using LC-tandem MS q

199 Furthermore, organ cultures of jejunal biopsies from 28 CD patients w

200 In contrast, under defined conditions in organ cultures of metanephric kidneys, c-kit-positive ce

201 yer cultures of human dermal fibroblasts and organ cultures of skin biopsies, were aimed at testing w

202 Using cell and organ cultures of the chick cochlea and utricle, we foun

203 Using in vitro organ cultures of the E16 UGS and P1 prostate, we show t

204 Fetal thymic organ cultures on the NOD background required 3- to 10-f

205 ine on normal human urothelium maintained in organ culture or as finite cell lines in vitro.

206 te hair growth modulatory agents in human HF organ culture or in clinical trials.

207 When monitored after skin organ culture or subcutaneous injection of tumor necrosi

208 ocytes, whether they are present in situ, in organ cultures or in tissue culture.

209 tinocytes or injected into mouse skin, human organ culture, or human xenografts detected TRAIL on ker

210 nesis and kidney organogenesis in an ex vivo organ culture/organoid setting.

211 and 2651 +/- 305 cells/mm(2) after 3 days in organ culture (P < .01).

212 aqueous humor outflow were determined using organ-cultured, perfused anterior segments of porcine ey

213 d in porcine eyes using the anterior segment organ culture perfusion system.

214 ave not been manipulated by either tissue or organ culture procedures.

215 In ccRCC organ cultures, R1-TNF increases TNFR1, activates apopto

216 In organ-cultured rat lenses, the peptides inhibited calcim

217 us Tgfbeta3 to the mutant palatal shelves in organ culture rescues the midline seam phenotype.

218 Tubular epithelial cells in normal kidney organ cultures respond to TNFR2 signaling by expressing

219 wever, choriocapillaris endothelial cells in organ culture responded to C5a by increasing ICAM-1 mRNA

220 lemented into miR-205-deficient fetal thymic organ cultures restored Foxn1 expression along with ccl2

221 Organ culture revealed reduced vascular endothelial grow

222 Mouse calvarial organ culture revealed that EGF elevated the number of p

223 immuno-histochemical (IHC) analyses of disc organ cultures revealed that IL-1 mediates changes in pr

224 Experiments using reaggregate thymic organ cultures revealed the presence of such precursors

225 fixed carbon, as suggested by research with organ-cultured roots.

226 In both explanted fibroblasts and skin organ cultures, rosiglitazone prevented the stimulation

227 In organ culture SDF-1 infiltrates cartilage and accelerate

228 primary mouse osteoblasts (PMOs) or in bone organ cultures) showed that MDA PCa 2b cells activated W

229 ZV strains to evaluate virus tropism in skin organ culture (SOC) and skin xenograft mouse models.

230 del using live mouse neonatal calvarial bone organ cultures stimulated by parathyroid hormone (PTH) t

231 ings combined with our studies on human skin organ cultures strongly indicate that the OR 2AT4 is inv

232 Organ culture studies indicate that expression of these

233 Organ culture studies show that ACTH-responsive cells ar

234 Prostate organ culture studies with a Mek inhibitor, U0126, and a

235 ce from mouse models and human hair-follicle organ-culture studies to explore the main pathobiology p

236 An ex vivo disc organ culture study supported that extracellular hyperos

237 her in embryos than in neonates, and ex vivo organ culture suggested that ectopic ureters can regress

238 as does downregulation of DSG1 in human skin organ culture, suggesting that it may have important sig

239 The use of a live bone organ culture system augmented these results with furthe

240 The organ culture system facilitated the identification of n

241 issue of Immunity, Hladik et al. describe an organ culture system for imaging HIV-1 interaction with

242 Using an organ culture system for prostate development and Ret mu

243 y a gamma-secretase inhibitor in an in vitro organ culture system of wild-type cochleae resulted in a

244 Here, we show in an ex vivo human organ culture system that upon contact in situ, HIV-1 ra

245 We devised a microfabricated organ culture system that viably preserves the normal mu

246 A recent study reports a novel organ culture system that will enhance our ability to di

247 In this study, we used an in vitro organ culture system to show that progesterone receptor

248 addition the transgenic line was used in an organ culture system to track spleen precursor cells dur

249 iments, particularly those using an in vitro organ culture system, demonstrated that these tissues pl

250 Importantly, using an ex vivo human organ culture system, we demonstrate the feasibility of

251 nation of genetically engineered mice and an organ culture system, we identified novel roles for fibr

252 nsional fibroblast-like synoviocyte in vitro organ culture system, we provide evidence that cadherin-

253 lity to induce bone resorption in an ex vivo organ culture system.

254 Hence, ex vivo organ culture systems allow pre-screening and pre-valida

255 Thereby, organ culture systems can reduce animal use, and improve

256 Recently, ex vivo three-dimensional organ culture systems have emerged to study the physiolo

257 e and knock-out whole embryonic kidney (WEK) organ culture systems was developed using 6-carboxyfluor

258 ying endosperm cell fates, a maize endosperm organ culture technique was established whereby the deve

259 tterns of cytopathology in tracheal rings in organ culture that had been previously ascribed to infec

260 where we have reported the use of ganglionic organ cultures that enable rapid reactivation in medium

261 ctivation, we examined the use of ganglionic organ cultures that enable rapid reactivation in medium

262 Functional studies in mouse organ cultures that faithfully reproduce the initiation

263 ical and clonal analysis in mice and cardiac organ culture, that coronary vessels arise from angiogen

264 re generated from human blood and intestinal organ cultures, then cocultured with naive and memory CD

265 In organ culture, TNF induced expression of E-selectin, VCA

266 In human kidney organ culture, TNFR2 signaling both upregulates TNFR2 ex

267 estigated by subjecting anterior segments in organ culture to an external magnetic field.

268 ed peptide ligands in transgenic mice and in organ culture to create thymic environments spanning a b

269 primordial follicle formation, we used ovary organ culture to inhibit and activate KIT signaling duri

270 have used chimeric human/mouse fetal thymic organ culture to study ADA-deficient human thymocyte dev

271 blishment and use of a novel nasal turbinate organ culture to study the initial steps of viral infect

272 ase inhibitor (SB431542) was used in palatal organ cultures to determine if blocking TFGbeta signalin

273 d attenuated IBV strains in ex vivo tracheal organ culture (TOC).

274 sured in perfused porcine and human anterior organ cultures treated with 20 or 50 mM sodium chlorate,

275 aBp65(Ser276), were obtained in mouse kidney organ cultures treated with cisplatin for 3 hours, sugge

276 in ovo using Shh-encoding retrovirus and in organ culture using recombinant protein, led to intestin

277 idogenic enzymes and GC synthesis in ex vivo organ cultures was studied in mouse lung tissue after in

278 With mutant mice and whole tongue organ cultures we demonstrate that Wnt5a protein and mes

279 Using a robust avian embryonic organ culture, we employ time-lapse two-photon laser sca

280 Using embryonic skin organ culture, we show that when skin from the sites of

281 f MRT techniques and a unique ex vivo testes organ culture, we show, for the first time, the MRT-medi

282 In this study, using fetal thymus organ cultures, we demonstrate that endogenous RA signal

283 n of in vitro gut slice cultures and ex vivo organ cultures, we determined the mechanistic role of LA

284 Using mycorrhizal root organ cultures, we manipulated the C supply to the host

285 Using hair follicle organ cultures, we show that FGF5 induces regression of

286 rneal epithelial cells in two-dimensional or organ culture were exposed to sidestream whole (SSW) smo

287 , possible confounders specific to avascular organ culture were investigated.

288 Human anterior segments in organ culture were perfused at a constant flow rate of 2

289 human TM cells and perfused anterior segment organ cultures were studied.

290 man retinal pigment epithelium (RPE)-choroid organ cultures were treated with AREDS multivitamin solu

291 assessed in vitro and using transgenic lens organ cultures, which identified the amide linked glucos

292 timulated calcification of mouse aortic ring organ cultures, which was suppressed by the TG2 catalyti

293 eatment of mouse Pkd1-null cystic kidneys in organ culture with a c-Met pharmacological inhibitor res

294 a(-/-) thymocytes transduced in fetal thymic organ culture with a retrovirus harboring CD3gammawt cDN

295 attenuation of Fgfr signaling in a calvaria organ culture with an Fgfr inhibitor prevents premature

296 Treatment of fibroblast-like synoviocyte organ cultures with a cadherin-11-Fc fusion protein effi

297 of chicken primary fibroblasts and tracheal organ cultures with chIFN-kappa imparted cellular protec

298 Treatment of mouse embryonic kidney organ cultures with TNF-alpha resulted in formation of c

299 40% (P < 0.05) in the human ocular perfusion organ culture without any observable changes in the morp

300 sis provided results that augmented the bone organ culture work and confirmed the capacity of phosvit