ライフサイエンスコーパス: organoid

1 monolayers) and 3D neuronal cultures (brain organoids).

2 romatin in human three-dimensional forebrain organoids.

3 TGR5 agonists promoted growth of intestinal organoids.

4 activities in human and mouse cell lines and organoids.

5 estinal crypts were isolated and cultured as organoids.

6 d-secreting parietal cells (PCs) in mice and organoids.

7 nto three-dimensional (3D) cerebral cortical organoids.

8 umber of Lgr5(+) stem cells in mouse colonic organoids.

9 rentiated epithelial monolayers from gastric organoids.

10 ug screens on human-derived pancreatic tumor organoids.

11 creased proliferation of patient-derived CRC organoids.

12 e in patient-derived xenografts and human PC organoids.

13 a, and cell-type specificity was retained in organoids.

14 signaling analysis of healthy and cancerous organoids.

15 generate a large repository of breast cancer organoids.

16 ysis of cells but is not yet compatible with organoids.

17 functional neuromuscular junctions in single organoids.

18 ithelial cells and viral entry in human lung organoids.

19 t also decreased spontaneous firing in these organoids.

20 ithelia both in vivo in mice and in vitro in organoids.

21 e H. pylori in the lumen of infected gastric organoids.

22 anio brains, hemispherotomy, and in cerebral organoids.

23 , to support routine passaging of intestinal organoids.

24 mary cell type in microdissected tubules and organoids.

25 m cell (hESC)-derived cardiac cells and lung organoids.

26 ganization in emerging model systems such as organoids.

27 ential for the growth of p53-deficient tumor organoids.

28 tion procedure and produce a large number of organoids (169 organoids per 3.5 cm x 3.5 cm device area

29 most frequently observed interaction, tumor organoids also actively constricted and displaced vessel

30 CRT-knockout organoids also had diminished barrier formation.

31 However, the current iterations of brain organoids also have limitations in faithfully recapitula

32 or and within-tumor statistical tests, using organoids analogously to large sibships and vastly ampli

33 eering approaches, along with newer unbiased organoid analysis readouts, should resolve several of th

34 ogenitor cells self-renewal capacity in both organoid and allograft assays.

35 function in vitro studies in patient-derived organoid and cell cultures as well as in vivo positron e

36 Here we used organoid and mouse models to determine the drivers of al

37 Organoids and adenomas were analyzed by quantitative rev

38 hanistically, coculture experiments of tumor organoids and CAFs showed that CAFs shape the epithelial

39 sing large patient datasets, patient-derived organoids and cancer cell lines, we identify mSWI/SNF su

40 f multiple cell classes in Zebrafish retinal organoids and cell distributions in mouse embryos.

41 le mouse organs and embryos, human biopsies, organoids and Drosophila.

42 to recapitulate brain development in neural organoids and focus on their applicability for disease m

43 r targets for studying disease mechanisms in organoids and for targeted repair in human retinas.

44 ight untreated and six METH-treated cerebral organoids and found that the organoids developed from em

45 We analyzed mouse liver organoids and HepG2 cells that expressed the FXR isoform

46 In organoids and in mice, both populations contribute equal

47 there is similarity between the trophoblast organoids and in vivo placentas in their transcriptomes

48 restomach tissues from mice were cultured as organoids and incubated with inhibitors of Notch or NF-k

49 Using human retinal organoids and mouse retina, we observed that a similar a

50 ate single-cell analysis of such networks in organoids and organoid cocultures.

51 Here, we combined organoids and Organs-on-Chips technology to create a hum

52 Single-cell RNA sequencing of both organoids and primary tissue identifies distinct venom-e

53 GLP-2 expanded intestinal organoids and reduced expression of apoptosis-related ge

54 thus conferring a malignant phenotype to the organoids and throughout the gut.

55 that differentially adjusts and conforms to organoids and tumoroids in a tissue-specific manner.

56 Comprehensive analysis of 46 intact organoids and ~ 100 million cells reveals quantitative m

57 Using primary cells, three-dimensional organoids, and CRISPR-Cas9 genome-edited JEG-3 clones, w

58 ultiple mammary epithelial cell types in the organoids, and demonstrate that protein expression patte

59 tudy, we utilize mice, small intestinal (SI) organoids, and ISC-Paneth cell co-cultured models to cla

60 or specimens, human pancreatic tumor-derived organoids, and organoids derived from tumors of KPC mice

61 ronal activity and calcium signaling in both organoid- and 2D-derived cortical neurons.

62 This genetic-, cellular-, organoid- and human-scale evidence underscored the polyg

63 ings demonstrate the utility of our in vitro organoid approaches for uncovering the early consequence

64 nal modification (PTM) signaling networks in organoids are absent.

65 s mouse patient-derived xenografts (PDX) and organoids are being developed to predict patient-specifi

66 son to fetal specimens suggest that the skin organoids are equivalent to the facial skin of human fet

67 Organoids are multicellular culture systems that replica

68 Organoids are powerful models for studying tissue develo

69 Cortical organoids are self-organizing three-dimensional cultures

70 In vitro, patient-derived cerebral organoids are smaller with a reduced number of prolifera

71 Intestinal organoids are useful in vitro models for basic and trans

72 onal (3D) cell culture technologies, such as organoids, are physiologically relevant models for basic

73 The study of organoids, artificially grown cell aggregates with the f

74 m and support the use of hiPSC-derived brain organoids as a platform to investigate SARS-CoV-2 infect

75 e results highlight the modeling of PCa with organoids as a powerful tool to elucidate the role of ge

76 Despite the widespread adoption of organoids as biomimetic tissue models, methods to compre

77 A three-dimensional organoid assay in colonic crypts isolated from CR-infect

78 n by flow cytometry, immunofluorescence, and organoid assays.

79 human primary brain tissue were observed in organoids at different developmental stages.

80 single-cell PTM analysis with thiol-reactive organoid barcoding in situ (TOBis) enables high-throughp

81 Here, we describe an organoid-based platform for functional studies of human

82 atsuzawa-Ishimoto et al report an intestinal organoid-based platform that re-creates genetic suscepti

83 Here we generate a living organoid biobank from patients with locally advanced rec

84 ere, we describe the first paediatric cancer organoid biobank.

85 macroscale tissues, akin to aggregate-based organoids, but with the critical advantage of harboring

86 mpression regulates the growth of intestinal organoids by modifying intracellular crowding and elevat

87 Neurodevelopment and Diseases Using 3D Brain Organoids, by Ai Tian, Julien Muffat, and Yun Li.

88 atric patients and show that patient-derived organoids can be expanded efficiently in vitro.

89 We also show that the organoids can model hypoxia-enhanced doxorubicin cardiot

90 The time required to isolate organoids can vary based on the tissue and organ type bu

91 d obeticholic acid to induce FXR activity in organoids, cell lines, and mice.

92 Tumor organoid cells derived from the TACSTD2(high) luminal ce

93 uate neuroendocrine differentiation of tumor organoid cells.

94 adial glial cells (RGCs), leading to smaller organoids characteristic of microcephaly.

95 re observed in 3D-cultured cell lines, tumor organoids, chemoresistant xenografts, syngeneic tumors a

96 l analysis of such networks in organoids and organoid cocultures.

97 inal sphingolipid, both in mice and in colon organoids (colonoids) generated from the distal colons o

98 Here, we consider the biology of brain organoids, compared and contrasted with the endogenous h

99 H. pylori-infected Smox(-/-) mice or gastric organoids, compared to infected wild-type animals or gas

100 ric adipose streaks contains lymphocyte-rich organoids comprised of a highly compacted leaf-like part

101 at, while existing nephron-containing kidney organoids contain distal nephron epithelium and no urete

102 ployed a multicellular 3D neurovascular unit organoid containing human brain microvascular endothelia

103 activated AKT1 (caAKT1), and c-Myc can form organoids containing versatile clinically relevant tumor

104 he mutant AIPL1 and PDE6 proteins in patient organoids, corroborating the findings in animal models;

105 that the radiation sensitivity of intestinal organoids could predict the sensitivity of individual pa

106 rm, feeder-free culture of human distal lung organoids, coupled with single-cell analysis, identifies

107 differentiation capability was evaluated by organoid culture.

108 Single-cell analyses of matched organoid cultures and native tissues by mass cytometry f

109 fetal organoids, revealing the maturation of organoid cultures in a dish.

110 the attrition of RGCs in developing retinal organoid cultures without compromising axon outgrowth, a

111 anogenesis, in the form of three-dimensional organoid cultures, and transformative new analytic techn

112 (2020) combine the power of analyzing organoid cultures, patient samples, and mouse models wit

113 isolates in well-differentiated human airway organoid cultures, was well tolerated (selectivity index

114 individual venom components is maintained in organoid cultures.

115 put comparison of signaling networks between organoid cultures.

116 n resistance in mouse models and in prostate organoid cultures.

117 Organoid derivation from dysplastic lesions facilitated

118 experimental platform comprised of cell and organoid derivatives from human pluripotent stem cells (

119 ition of RSPO1 reduced the number of adenoma organoids derived from Apc(Min/+) mice and suppressed ex

120 e or acetate to the TCA cycle was reduced in organoids derived from Hnf4alphagamma(DKO) mice, compare

121 For vision science, retinal organoids derived from human stem cells allow differenti

122 e, InsP(3) also induced growth of intestinal organoids derived from human tissue, stimulated HDAC3-de

123 tosis or interferon signaling protects human organoids derived from individuals harboring a common AT

124 Brain organoids derived from induced pluripotent stem cells (i

125 Organoids derived from mouse and human stem cells have r

126 ulture system for distal lung progenitors as organoids derived from single adult human alveolar epith

127 ant activity of a pan FGFR inhibitor against organoids derived from the FGFR1-ERLIN2 fusion-positive

128 uman pancreatic tumor-derived organoids, and organoids derived from tumors of KPC mice, compared with

129 In contrast, organoid-derived epithelial monolayers expressed markedl

130 reated cerebral organoids and found that the organoids developed from embryonic stem cells contained

131 als quantitative multiscale "phenotypes" for organoid development, culture protocols and Zika virus i

132 hroughput format and induced to form hepatic organoids; development of functional bile canaliculi was

133 ATG16L1 knockout (KO) and NOD2 KO organoids did not benefit from the MDP-induced cytoprote

134 tina as well as four early stages of retinal organoid differentiation.

135 In parallel, cellular reprogramming and organoid engineering are expanding the use of human neur

136 growth of neratinib-resistant xenografts and organoids established from neratinib-resistant PDXs.

137 s are superior to Wnt3a conditioned media in organoid expansion and single-cell organoid outgrowth.

138 Thus, NGS Wnts offer a unified organoid expansion protocol and a laboratory "tool kit"

139 Using organoid experiments, we identified a molecular mechanis

140 riptome of the Cape coral snake reveals that organoids express high levels of toxin transcripts.

141 Here, we provide an overview of the cerebral organoid field and speculate how engineering strategies,

142 Moreover, we show that skin organoids form planar hair-bearing skin when grafted ont

143 organoids stimulated with RSPO1-Fc restored organoid formation and expression of genes regulated by

144 ells have multipotent progenitor activity in organoid formation and tissue reconstitution assays.

145 o promote cellular adhesion/colonization and organoid formation by controlling the p53 protein level.

146 a proliferative gene signature and promotes organoid formation from progenitor cells and enhances re

147 st-Luminal-C) exhibited greater capacity for organoid formation in vitro and prostate epithelial duct

148 cell colonies without loss in morphology or organoid formation potential.

149 roliferation, migration, differentiation and organoid formation.

150 We derived 8 organoids from 8 EAC tissues and tested their sensitivit

151 Cerebral organoids from AD patients carrying APOE epsilon4/epsilo

152 Organoids from colon cells of CRT-knockout mice and cont

153 The ex vivo generation of gastrointestinal organoids from crypt stem cells opens up the possibility

154 We established organoids from duodenal crypts, incubated them with labe

155 ST6Gal-I, we generated human gastric antral organoids from epithelial stem cells and differentiated

156 imized a protocol to generate choroid plexus organoids from hiPSCs and showed that productive SARS-Co

157 tform to assemble and culture human cerebral organoids from human embryonic stem cells (hESC) to inve

158 We previously generated liver organoids from iPSCs, namely iPSC-liver buds (iPSC-LBs),

159 eneic T cells kill Atg16L1-mutant intestinal organoids from mice, which was associated with an aberra

160 in primary hepatocytes and stem cell-derived organoids from multiple donors treated with over ten dif

161 rotocols to isolate and culture heterogenous organoids from murine and human primary and metastatic s

162 ll line and patient-derived tumor and normal organoids from two breast cancer patients using Illumina

163 ailed procedures for generating glioblastoma organoids (GBOs) from surgically resected patient tumor

164 that might be responsible for the different organoid gene expression thus accounting for a deranged

165 nuclein in the culture medium of human brain organoids generated from normal and idiopathic PD patien

166 g, we employed cortical neurons and cerebral organoids generated from PITRM1-knockout human induced p

167 On the other hand, over time, cerebral organoids generated from PITRM1-knockout iPSCs spontaneo

168 We discuss the emergence of brain organoids generated from pluripotent stem cells as a mod

169 Here, we review recent advances in brain organoid generation and characterization with a focus on

170 Brain organoids grown from human pluripotent stem cells self-o

171 ronchioles and exhibited enriched clonogenic organoid growth activity.

172 Inhibitors of YAP1 and SRC prevented organoid growth induced by TGR5 activation.

173 ISC) proliferation and is a key component of organoid growth media yet is dispensable for intestinal

174 stem cell expansion, and promote intestinal organoid growth.

175 ell markers, and a failure of in vitro crypt organoid growth.

176 rve-like bundles that target Merkel cells in organoid hair follicles, mimicking the neural circuitry

177 e past decade, human stem cell-derived brain organoids have emerged as a biologically relevant model

178 Organoids have great potential to improve our current un

179 ing element activity between fetal brain and organoids have helped identify gene regulatory networks

180 However, IEC organoids have increased expression of the type I IFN re

181 re models, such as ex vivo culture models or organoids, have also been developed over the past decade

182 veloped a three-dimensional (3D) human brain organoid (hBORG) model containing major cell types impor

183 ripotent stem cell (hPSC)-derived intestinal organoids (HIOs) lack some cellular populations found in

184 ance, and optical analysis of human midbrain organoids in standard 96-well-plates.

185 mbled these organoids with cerebral cortical organoids in three-dimensional cultures to form cortico-

186 varying existing organisms or bioengineering organoids in vitro.

187 oliferation in crypts and induces budding in organoids, in part through elevated and sustained activa

188 erm expansion of multiple different types of organoids, including kidney, colon, hepatocyte, ovarian,

189 oc2, Msi1, and Ascl2) were down-regulated in organoids incubated with etomoxir, an inhibitor of FAO,

190 Organoids incubated with free fatty acids had gene expre

191 n reaction and RNAscope) of small intestinal organoids incubated with the Notch inhibitor DAPT, intes

192 d intestinal metaplasia and in human gastric organoids infected with H pylori.

193 ercentage of colonic stem cells and enhances organoid initiating capacity and growth of sorted stem a

194 Imaging revealed that tumor organoids integrate into the endothelial cell lining, re

195 leads to differentiation of the trophoblast organoids into HLA-G+ EVT cells which rapidly migrate an

196 or-specific (reproducible between individual organoids/iPSC lines/experiments).

197 hat productive SARS-CoV-2 infection of these organoids is associated with increased cell death and tr

198 s in animal models; however, patient-derived organoids maintained retinal cell cytoarchitecture despi

199 we show that these AD neuronal cultures and organoids manifest increased spontaneous action potentia

200 Cell types in organoids matured in vitro to a stable "developed" state

201 However, current organoid methods are limited by interior hypoxia and cel

202 In a human sebaceous organoid model GATA6-mediated down-regulation of the inf

203 asal cells and establishes a facile in vitro organoid model of human distal lung infections, includin

204 s in meningioma, we develop a human cerebral organoid model of meningioma and validate the high ADC m

205 This 3D organoid model recapitulates characteristics of BBB dysf

206 s the respiratory tract, we developed a lung organoid model using human pluripotent stem cells (hPSC-

207 ell cultures and three-dimensional forebrain organoid model.

208 Cortical brain organoid modeling shows reduced proliferation of radial

209 ri et al., 2020) leverage novel glioblastoma organoid models and single-cell RNA-sequencing technolog

210 h level of mammographic density (MD) and the organoid models are exposed to 50 cGy X-ray radiation.

211 Stem cell-derived organoid models have emerged as a valuable tool for stud

212 sed stiffness of the microenvironment in the organoid models of premalignant human mammary cell lines

213 oscopy, to monitor neurodevelopment in brain organoid models that can complement traditional drug des

214 combination with in vivo lineage tracing and organoid models to finely map the trajectories of alveol

215 These findings exploit murine organoid models to uncover the mechanism of ERG-mediated

216 he underlying mechanism, we develop cerebral organoid models using induced pluripotent stem cells (iP

217 ell growth in cell lines and patient-derived organoid models.

218 12D); Trp52(R172H/+); Pdx-1 Cre murine tumor organoids models.

219 preferentially to the basolateral surface of organoid monolayers and caused increased cell vacuolatio

220 ry of epithelial shells which resemble small-organoid morphologies.

221 testine tissues of mice and human intestinal organoids, MTG8 and MTG16 repress transcription in the e

222 to the hydrogel properties tailored for the organoid of interest.

223 at can be used to reliably generate cerebral organoids of a telencephalic identity and maintain long-

224 omplex but highly heterogeneous aggregates ('organoids') or 3D structures with less physiological rel

225 media in organoid expansion and single-cell organoid outgrowth.

226 Patient-derived organoids (PDO) obtained from tumors with high AXL and J

227 ting evidence indicates that patient-derived organoids (PDOs) can predict drug responses in the clini

228 and produce a large number of organoids (169 organoids per 3.5 cm x 3.5 cm device area) without fusio

229 We developed a hepatic organoid platform with human cells that can be used to m

230 on led to the differentiation of airway-like organoids possessing functional basal cells capable of c

231 ould support long-term culture of intestinal organoids, potentially replacing the need for animal-der

232 Human brain organoids provide unique platforms for modeling developm

233 inical trial data confirm that rectal cancer organoids (RCOs) closely recapitulate the pathophysiolog

234 Human organoids recapitulating the cell-type diversity and fun

235 h cells within three-dimensional co-cultured organoids, removing the need for the restrictive synthet

236 Here, we derive organoids resembling the cerebral cortex or the hindbrai

237 ches needed to leverage both fetal brain and organoid resources promise to answer major questions of

238 Paediatric kidney tumour organoids retain key properties of native tumours, usefu

239 riptomic and proteomic signatures of ChP-CSF organoids reveal a high degree of similarity to the ChP

240 ngle-cell analysis of KRT5(+) cells in basal organoids revealed a distinct population of ITGA6(+)ITGB

241 scRNA-seq analysis of the organoids revealed enhanced GABAergic specification and

242 ptomes of ex vivo tissues and in vitro fetal organoids, revealing the maturation of organoid cultures

243 n single cells derived from small intestinal organoids reveals cell-type- and cell-state-specific sig

244 we generated three-dimensional (3D) retinal organoids (ROs) from human induced pluripotent stem cell

245 pluripotent stem cell (iPSC)-derived retinal organoids (ROs) transcriptome analysis.

246 We found that T21 organoids secrete increased proportions of Abeta-prevent

247 red with human intrafusal muscle fibers, DRG organoid sensory neurons contact their peripheral target

248 Conversely, 3D brain organoids showed accumulation of Abeta42 mainly in non-i

249 Here, we report the sliced neocortical organoid (SNO) system, which bypasses the diffusion limi

250 Inhibition of TGFBR signaling in organoids stimulated with RSPO1-Fc restored organoid for

251 However, organoid stress and subtype defects are alleviated by tr

252 murine lung stem cell-based bronchioalveolar organoid system and provide insights into the effect of

253 erformed using human cell lines and a murine organoid system.

254 ically engineered mouse models, we developed organoid systems from primary mouse and human induced pl

255 a cell type heterogeneity is comparable with organoid systems, and teratoma gut and brain cell types

256 vo neurobiology, and further improvements in organoid techniques are continuing to narrow the gap wit

257 diting, imaging, and genomic analysis, brain organoid technology can be applied to address questions

258 tion will be instrumental for translation of organoid technology for clinical applications as well as

259 Recently, organoid technology has been used to generate a large re

260 Recent advances in brain organoid technology have created new opportunities to in

261 in genome engineering and the various omics, organoid technology is making possible studies of human

262 blished an in vitro mouse PCa model based on organoid technology that takes into account the cell of

263 ve, we evaluate the recent progress in brain organoid technology, and discuss the experimental consid

264 these findings will help to underpin retinal organoid technology, which holds much promise as a platf

265 generation of glucose-responsive islet-like organoids that are able to avoid immune detection provid

266 -wide binding of FXR isoforms in mouse liver organoids that express individual FXR isoforms using chr

267 t and reduced insulin-induced lipogenesis in organoids that expressed FXRalpha2 but not FXRalpha1.

268 Here we show that cardiac organoids that incorporate an oxygen-diffusion gradient

269 ons, such adult stem cells (ASCs) grow as 3D organoids that recapitulate essential features of the pe

270 in MeCP2 mutant region-specific human brain organoids that were rescued by JQ1.

271 in activation was confirmed in human gastric organoids that were treated with a novel SMOX inhibitor.

272 lso show, using both mouse and human colonic organoids, that TcdB from epidemic ribotype 027 strains

273 in the response of individual patient-based organoids to drug treatments and find that temporally-mo

274 Here we expose human intestinal organoids to genotoxic pks(+) E. coli by repeated lumina

275 and highlight experimental strategies to use organoids to pioneer new understanding of human brain pa

276 cessful use of rectal cancer patient-derived organoids to predict patient responses to neoadjuvant ch

277 eature profiles for hundreds of thousands of organoids to quantitatively describe their phenotypic la

278 ive to neonate IECs, and the response of IEC organoids to type I IFN is strikingly increased in magni

279 tensive collection of datasets and describes organoid tools to study the transcriptional and proteomi

280 ession, we show that expansion of intestinal organoids was facilitated through elevated Wnt/beta-cate

281 lation of Abeta42 in ICP4+ cells in infected organoids was rarely observed.

282 By employing gastrointestinal 3D organoids, we detect that ERK3 protein levels steadily d

283 ngle-cell RNA sequencing and synovial tissue organoids, we found that NOTCH3 signalling drives both t

284 Organoids were analyzed for mitochondrial respiration, l

285 Organoids were cultured in hypoxic chamber with 0.1% O(2

286 Organoids were incubated with oleic and palmitic acid, a

287 s with unmodified hPSCs to generate cerebral organoids, which expedited in situ myelination.

288 report the successful creation of human ChP organoids, which produce a cerebrospinal fluid-like secr

289 We anticipate that our skin organoids will provide a foundation for future studies o

290 Here, we establish human ChP organoids with a selective barrier and CSF-like fluid se

291 We created distal lung organoids with apical-out polarity to present ACE2 on th

292 We then assembled these organoids with cerebral cortical organoids in three-dime

293 Incubation of organoids with free fatty acid-enriched media resulted i

294 ut-sterilized p53-mutant mice and p53-mutant organoids with gallic acid reinstated the TCF4-chromatin

295 stratified patient-derived colorectal cancer organoids with known Ras mutational status according to

296 al cells (IEC) derived from human intestinal organoids with monocyte-derived macrophages, in a gut-on

297 Organoids with reduced levels of MGMT and CHFR expressio

298 We challenge organoids with SARS-CoV-2 spike pseudovirus and live vir

299 In vitro treatment of IEC organoids with type III IFN results in ISG expression th

300 y intravital microscopy of colorectal cancer organoid xenografts to investigate the cell of origin of