ライフサイエンスコーパス: ouabain

1 0.9 versus 1.6 +/- 0.5 mmHg with intravenous ouabain).

2 /K(+) -ATPase (l-NMMA + ketorolac + BaCl2 + ouabain).

3 ) channels and Na(+) /K(+) -ATPase (BaCl2 + ouabain).

4 c; and L-NMMA plus ketorolac plus BaCl2 plus ouabain.

5 podocyte primary cell cultures with low-dose ouabain.

6 before or after administration of BaCl2 plus ouabain.

7 ively inhibiting alpha2 enzyme activity with ouabain.

8 e measured with and without NKA inhibited by ouabain.

9 ensitive to the Na+/K+-ATPase pump inhibitor ouabain.

10 of action potential fall in the presence of ouabain.

11 of Na/K-ATPase, like the pumping pump, binds ouabain.

12 umps were silenced by continuous exposure to ouabain.

13 /K pump and the site of its interaction with ouabain.

14 ent arteriole to contract in the presence of ouabain.

15 n ADPKD cells exhibits a higher affinity for ouabain.

16 This effect was blocked by ouabain.

17 nal Na+/K+-ATPases induced by the binding of ouabain.

18 f lithium on V(m) was virtually abrogated by ouabain.

19 ibition of FGF2 secretion in the presence of ouabain.

20 zation of the pump or affect the response to ouabain.

21 TPase with subtoxic doses of gramicidin A or ouabain.

22 by comparison with digoxin, digoxigenin, or ouabain.

23 s of these experiments showed that 20 microM ouabain, 0.3 microM ionomycin, or their combination incr

24 Ouabain (10 mumol l(-1)), a specific inhibitor of Na/K-A

25 Here, we assess whether acute (75 muM ouabain 100 nM blebbistatin) or chronic myocardial Na(i)

26 in the presence of Na(+)/K(+) pump-inhibitor ouabain (100 uM), was significantly smaller in ChAc neur

27 Ouabain (3 nm) also increased ER Ca(2+) release and enha

28 Ouabain (3 nm) pre-incubation also augmented 10 muM cycl

29 urve) was attenuated by BaCl2 (-61+/-3%) and ouabain (-44+/-12%) alone, and this effect was enhanced

30 Cl(2) toxicity versus ion pump inhibition by ouabain), a significant advance against state of the art

31 Suppression of autosis by ouabain, a cardiac glycoside, in humanized Na+,K+-ATPase

32 unction of Na(+),K(+)-ATPase is activated by ouabain, a mammalian steroid hormone, at far lower conce

33 sistance in other species to the cardenolide ouabain, a plant toxin capable of blocking ATPases.

34 2V vs. Ag/AgCl in the presence or absence of ouabain, a specific NKA inhibitor.

35 Ouabain, a steroid present in the circulation and in var

36 bition of the NKA alpha2 isoform by low dose ouabain abolished the ability of low Ko to reduce NKA cu

37 Ouabain, acting from the basolateral side of the cells,

38 Ouabain activated the NF-kappaB antiapoptotic subunit p6

39 two mutants caused significant inhibition of ouabain-activated signal transduction and cell growth.

40 Ouabain administration also caused activation of Akt, de

41 ptor function as evidenced by the failure of ouabain administration to induce the activation of Src a

42 The increased ouabain affinity of ADPKD cells was not due to differenc

43 loop, known to make a major contribution to ouabain affinity, strongly influenced conductance of sin

44 d astrocytes express Na(+) pumps with a high-ouabain-affinity catalytic subunit (alpha3 and alpha2, r

45 brain as a result of its actions on the high-ouabain-affinity Na(+) pumps in both neurones and astroc

46 Treatment of hTMC with sodium fluoride or ouabain, agents shown to cause morphological changes to

47 Ouabain alone reduced pH(i) recovery, but SNP+ouabain ca

48 Treatment with 20 microM ouabain also increased the ultimate tensile strength of

49 By binding to the Na,K-ATPase, ouabain also induces proliferation in some cell types.

50 This suggests that ouabain also inhibits an ion resorptive function of Na/K

51 ns, resulting from intravitreal injection of ouabain, also exhibited increased TNFalpha expression th

52 at inhibit Na,K-ATPases, such as digoxin and ouabain, alter cardiac myocyte contractility.

53 ibitor of cytochrome-P450 enzymes or dihydro-ouabain, an inhibitor of Na+,K+-ATPase blocked the effec

54 was phenocopied by treatment of embryos with ouabain, an inhibitor of Na,K-ATPase activity.

55 racerebroventricular (ICV) administration of ouabain, an inhibitor of the Na, K-ATPase, is an approac

56 onensin, a Na(+) ionophore, with and without ouabain, an NKA inhibitor, in suspensions of human eryth

57 vity to the electrolyte transport inhibitors ouabain and bumetanide, as well as bath Cl(-) and HCO(3)

58 analysis of model bioactive/toxic compounds (ouabain and CdCl(2)) demonstrates that cellular reactivi

59 We further characterized ouabain and demonstrated that it inhibits sFlt1 mRNA and

60 ied a group of cardiac glycosides, including ouabain and digoxin, as potent inhibitors of NMD.

61 veral cardiotonic steroids (CTSs), including ouabain and digoxin, increased RGS2 but not RGS4 protein

62 extracellular K(+) did not reduce p53 as did ouabain and digoxin, it did potentiate both digoxin- and

63 CX) blocker, antagonized the effects of both ouabain and digoxin.

64 mally sensitive to the Na+/K+ pump inhibitor ouabain and exhibited age-dependent changes, including d

65 In contrast, ouabain and extracellular K(+) failed to produce detecta

66 ies, we used the Na(+)/K(+) ATPase inhibitor ouabain and found conditions in which SD was always prev

67 Fluid absorption was sensitive to ouabain and gadolinium but insensitive to benzamil, bafi

68 Ouabain and ionomycin may be useful pharmacologic agents

69 The enzyme is strongly inhibited by ouabain and its derivatives, some that are therapeutical

70 reduced peak FBF (-50+/-6%; P<0.05), whereas ouabain and L-NMMA plus ketorolac did not.

71 The effects of ouabain and low-Na(+) solutions were determined under op

72 We have also determined the effects of ouabain and reduced bath [Na(+)].

73 demonstrated by reduction of the current by ouabain and the K(+) channel blockers Ba(2+), XE991, and

74 d by the beta-surface of the steroid core of ouabain and the side chains of alphaM1, alphaM2, and alp

75 e, or combined NO, PGs, Na(+) /K(+) -ATPase (ouabain) and KIR channel inhibition (n = 6; Protocol 2).

76 ic steroids (the Na(+)/K(+)-ATPase inhibitor ouabain), and corticosteroids (dexamethasone).

77 were cardiac glycosides, including digoxin, ouabain, and proscillaridin A, which inhibited HIF-1alph

78 n carbonylation is reversed after removal of ouabain, and this reversibility is largely independent o

79 -interacting pool of Src and act as a potent ouabain antagonist in cultured cells: 1) pNaKtide, unlik

80 that intraperitoneal administration of anti-ouabain antibodies to pregnant mice resulted in a >80% d

81 Cardiotonic steroids, such as ouabain, are specific inhibitors of the Na/K pump.

82 rbonate media through physical trauma, 10 mM ouabain as Na+/K+ ATPase ion transport inhibitor, or 1 m

83 bule of the E2P model explains high-affinity ouabain binding in a mutant of the H,K ATPase.

84 and ouabain-resistant mutation of the alpha2 ouabain binding site (alpha2(R/R) mice) confers resistan

85 he CTS binding pocket of [Mg]E2P allows deep ouabain binding with possible long-range interactions be

86 d the cation site and thereby hindering deep ouabain binding.

87 ypes of cells, may function as non-canonical ouabain-binding receptors.

88 cardiotonic steroids, which act through the ouabain-binding site and are important in cardiovascular

89 The amino acids that constitute the ouabain-binding site are highly conserved across the evo

90 These data suggest that the ouabain-binding site of the alpha1 Na,K-ATPase can parti

91 is important to characterize the role of the ouabain-binding sites in this context.

92 lute and water transport in ADPKD cells, and ouabain blocks fluid secretion in these cells.

93 triggered by a conformational change of the ouabain-bound Na(+)/K(+)-ATPase molecule or more general

94 structures of Na,K-ATPase in the unbound and ouabain-bound states.

95 T inhibitors and the Na+/K+-ATPase inhibitor ouabain, but not 5-HT2 and 5-HT1B/1D receptor inhibitors

96 ht: prolonged subcutaneous administration of ouabain, but not digoxin, induces hypertension, and digo

97 of hypertension, antagonized the effects of ouabain, but not digoxin.

98 Taken together, these results suggest that ouabain can protect the kidney from the apoptotic effect

99 uabain alone reduced pH(i) recovery, but SNP+ouabain caused significant further reduction.

100 tammetry showed that administration of 50 uM ouabain causes a drastic drop in dopamine uptake rate, c

101 On the other hand, ouabain causes a gradual depletion of Na/K-ATPase and an

102 iotonic steroids, most notably the digitalis/ouabain class of compounds, which have been used for cen

103 g kinetics with the previously described E2P-ouabain complex to derive specific details and the gener

104 g(2+) is bound in site II of the digoxin and ouabain complexes, whereas both sites are occupied by K(

105 sh retina by intravitreal injection of a low ouabain concentration to rapidly damage the ganglion cel

106 Although much higher ouabain concentration was required to stimulate Src in I

107 of the sensitivity of the alpha2 isoform to ouabain, conferring ouabain sensitivity to alpha1 augmen

108 resistant mice were inhibited by intravenous ouabain (control stop-flow pressure = 9.7 +/- 0.9 versus

109 e with 3 drugs (veratridine, brevetoxin, and ouabain) could discriminate wild-type channels from gain

110 nse light and recovered for up to 8 weeks or ouabain-damaged retinas that recovered for up to 3 weeks

111 rate at detecting the inner nuclear layer in ouabain-damaged retinas, but accurately detected the und

112 ATP12A inhibition by ouabain decreased bacterial proliferation.

113 Ouabain decreased glycolysis by 25% and decreased (14)CO

114 ents, loop of Henle perfusion with 50 microM ouabain decreased TGF responses (control stop-flow press

115 evidence points to a role of Na,K-ATPase in ouabain-dependent signal transduction.

116 Ouabain-dependent signaling is thought to be mediated wi

117 ng requires reassessment of the mechanism of ouabain-dependent signaling.

118 ing various cardiac glycoside compounds like ouabain, digoxin, digitoxin, and gitoxin with their agly

119 eyes that were injected intracamerally with ouabain, disodium 4,4'-diisothiocyanatostilbene-2,2'-dis

120 Ouabain down-regulated sFlt1 production by inhibiting hy

121 Pre-incubation (0.5-10 min) with 1-10 nm ouabain (EC50 < 1 nm) augmented Glu- and CCh-evoked sign

122 tion and cell proliferation, suggesting that ouabain effect is mediated through the MEK-ERK pathway.

123 rd E2 species reduced glutathionylation, and ouabain eliminated a ONOO(-)-induced increase.

124 reased by inhibition of the Na(+)/K(+) pump (ouabain), ENaC (amiloride), CF transmembrane conductance

125 ted by both brain and circulating endogenous ouabain (EO).

126 one another in both assays: For example, the ouabain-evoked (3 nm) increases in MT70 and neuronal Ca2

127 TPase in complex with the CTS representative ouabain, extending to 3.4 A resolution.

128 NaKtide may be used as a novel antagonist of ouabain for probing the physiological and pathological s

129 , a digoxigenin derivative that displaces 3H-ouabain from Na+, K+ -ATPase, and attenuates some forms

130 Ouabain further reduced glutathionylation in E2 and elim

131 3 compounds in the cardiac glycoside family, ouabain, gitoxigenin, and digitoxin, that inhibit placen

132 Ouabain had similar effects to those of MBG, suggesting

133 inding abolished the antiapoptotic effect of ouabain in Stx2-exposed tubular cells.

134 related to the presence of plants containing ouabain in the geographic locations where both inversion

135 ated the involvement of maternal circulating ouabain in the regulation of fetal growth and organ deve

136 tubular cells, cardiotonic steroids such as ouabain in vitro signal through Na/K-ATPase, which resul

137 ut was evoked using dorsal-root stimulation, ouabain increased burst frequency and extended locomotor

138 The sodium pump inhibitor, ouabain, increased the frequency and decreased the ampli

139 Ouabain increases the endocytosis and degradation of Na/

140 The O2 dependence of ouabain-independent K+ transport mechanisms has been stu

141 aft model of retinoblastoma, the cardenolide ouabain induced complete tumor regression in the treated

142 In this system, ouabain induced Na/K-ATPase/c-Src signaling and redistri

143 cal inhibition of the Na(+)/K(+)-ATPase with ouabain induced neurite degeneration, which was attenuat

144 -K(+) ATPase antagonists (ouabain or dihydro-ouabain) induced either a membrane depolarization in cur

145 ly, both Src and caveolin-1 are required for ouabain-induced activation of Akt and up-regulation of N

146 Concomitantly it also abolished ouabain-induced activation of Src and ERK1/2.

147 Consequently, it blocks ouabain-induced activation of Src, ERK, and hypertrophic

148 d Ca2+ transients were augmented, and 100 nM ouabain-induced amplification was abolished.

149 Here we show that ouabain-induced cell growth regulation is intrinsically

150 fish alpha1a.1 or rat alpha1 mRNA, while the ouabain-induced defect was rescued by expression of ouab

151 kinase kinase (MEK) inhibitor U0126 blocked ouabain-induced ERK activation and cell proliferation, s

152 /mTOR pathway by rapamycin completely blocks ouabain-induced expression of Na/K-ATPase and converts o

153 duced expression of Na/K-ATPase and converts ouabain-induced growth stimulation to growth inhibition

154 Na(+)] was increased to 20 mm or more (using ouabain-induced inhibition of the Na(+),K(+)-ATPase or t

155 damage and regeneration of photoreceptors or ouabain-induced inner retinal damage.

156 nlike previous studies in other systems, the ouabain-induced internalization was insensitive to Src o

157 This newly developed mouse model of ouabain-induced mania-like behavior could provide a pers

158 We describe a mouse model of ouabain-induced mania-like behavior.

159 digoxin, it did potentiate both digoxin- and ouabain-induced p53 reduction in sensitive lines.

160 -ATPase/Src receptor complex and antagonizes ouabain-induced protein kinase cascades.

161 he Na/K-ATPase affect basal Src activity and ouabain-induced signal transduction.

162 In alpha1-resistant/alpha2-resistant mice, ouabain infusion had no effect on TGF responses.

163 Prolonged ouabain infusion induces hypertension in rodents, and ou

164 However, ouabain-inhibitable respiration did not decrease during

165 Ouabain inhibited the FF from either side of the prepara

166 ility of digoxin-like CTSs to reactivate the ouabain-inhibited complex can be explained by de-oligome

167 st to NHK cells, the dose-response curve for ouabain inhibition of Na,K-ATPase activity indicated tha

168 The implication is that nanomolar ouabain inhibits alpha3 Na(+) pumps, increases (local) i

169 Ouabain-initiated phosphorylation of Ser(16) alpha1 was

170 ine and ganglion cells were lost by 7 d post-ouabain injection (dpi).

171 By 24 h after ouabain injection, maximal numbers of terminal deoxynucl

172 After ouabain injection, receptor and postreceptor uptake of m

173 wildtype zebrafish at 0, 3 and 18 days after Ouabain injection.

174 rgeted mouse (alpha2(R/R)) which expresses a ouabain-insensitive alpha2 isoform of the Na,K-ATPase to

175 Overexpression of ouabain-insensitive rat alpha1 failed to inhibit interna

176 specific reduction in NCX1 protein and were ouabain-insensitive.

177 ration of 0.5 ul of 50 uM (25 pmol, 14.6 ng) ouabain into each lateral brain ventricle results in inc

178 These results support the notion that ouabain is a growth factor and suggest that a reduction

179 Endogenous ouabain is an adrenal stress hormone associated with adv

180 e effects is heightened by the evidence that ouabain is endogenous in mammals.

181 The final model shows that ouabain is trapped within the external ion permeation pa

182 nd Ser(16), respectively, the latter because ouabain itself increased Ser(16) phosphorylation; thus,

183 After the reduction in maternal circulating ouabain, kidney expression of cyclin D1 was reduced and

184 /K(+)-ATPase, NO, and prostaglandins (BaCl2, ouabain, L-NMMA [N(G)-monomethyl-L-arginine] and ketorol

185 ng human pregnancy, the circulating maternal ouabain level increased and the highest concentration of

186 esulted in a >80% decline in the circulating ouabain level.

187 Preoperative plasma endogenous ouabain levels are powerful biomarkers of acute kidney i

188 Furthermore, circulating ouabain levels in women with small-for-gestational age n

189 Most CTSs could be divided into ouabain-like (ouabagenin, dihydroouabain (DHO), strophan

190 n each group, the CTSs were synergistic, but ouabain-like and digoxin-like CTSs antagonized one anoth

191 hat preoperative plasma levels of endogenous ouabain may function as this type of biomarker.

192 Thus, nanomolar ouabain may strongly influence synaptic transmission in

193 The ouabain-mediated downregulation of GlyT2 also occurs in

194 in a feed-forward mechanism of regulation of ouabain-mediated renal proximal tubule Na/K-ATPase signa

195 Bcl-xL, an inhibitor of Bax, suggesting that ouabain might protect renal cells from Stx2-triggered ap

196 w and high levels of the specific NKA ligand ouabain modulate the endocytosis and total expression of

197 Using a systematic approach, we applied ouabain (Na(+)/K(+)-ATPase inhibitor), bumetanide (Na(+)

198 red due to the very slow dissociation of the ouabain.Na(+)/K(+)-ATPase complex.

199 Signaling via the ouabain/Na,K-ATPase/IP3R/NF-kappaB pathway increases exp

200 We tested nanomolar ouabain on Ca(2+) signalling (fura-2) in rat hippocampal

201 ase dictate the growth regulatory effects of ouabain on cells.

202 enal parameters in rats and the influence of ouabain on podocyte proteins both "in vivo" and "in vitr

203 rolonged elevations of circulating exogenous ouabain on renal parameters in rats and the influence of

204 G and a similar Na(+)/K(+)-ATPase inhibitor, ouabain, on the phosphorylation status of Jnk, p38, and

205 perfusion of Na(+)-K(+) ATPase antagonists (ouabain or dihydro-ouabain) induced either a membrane de

206 Preincubation of lenses with either ouabain or low-[Na(+)] solutions resulted in reduced rat

207 e pumps that have been blocked by endogenous ouabain or other cardiac glycosides.

208 lace-cell-train-induced AHPs were blocked by ouabain or removal of extracellular potassium, but not b

209 a1 and alpha3 isoforms of Na(+)/K(+)-ATPase, ouabain or strophanthidin, inhibited this Na(+) current.

210 This delay was abolished by ouabain or zero K(+) saline, which eliminate the usAHP.

211 hate-buffered saline (PBS, vehicle) or PBS + ouabain, or after intraperitoneal injection of sodium io

212 r calcium, but were blocked by tetrodotoxin, ouabain, or the removal of extracellular potassium.

213 Here we tested the ability of ouabain (OUA, 3 microm), digoxin (DIG, 20 microm) or ace

214 ot different than that observed for combined ouabain plus BaCl(2) administration.

215 verage 56 +/- 5% (n = 16) following combined ouabain plus BaCl(2) infusion.

216 lated by cardiac glycoside drugs digoxin and ouabain, potent inhibitors of Na(+)/K(+)-ATPase.

217 A Na(+)/K(+)-ATPase inhibitor (ouabain) potentiated EA-induced cytotoxicity and direct

218 e survival factor Bcl-xL; co-incubation with ouabain prevented all of these effects.

219 Bound ouabain protects the site with the strongest influence o

220 Group 2 received ouabain rather than BaCl2 in the second trial.

221 The Na,K-ATPase inhibitor ouabain reduced and enhanced the carbachol-promoted phos

222 ally, in the rat model, elevated circulating ouabain reduced creatinine clearance (-18%, p < 0.05), i

223 rat model of pregnancy-induced hypertension, ouabain reduced mean arterial pressure and enhanced plac

224 arities in the Atpalpha variants, conferring ouabain resistance in both arrangements, may be the resu

225 Ouabain-resistance test detecting mutations in the Na(+)

226 lpha1 isoform of mice and rats is relatively ouabain resistant, gene-targeting strategies were used t

227 e alpha1 isoform mice were equivalent to the ouabain-resistant alpha1 isoform mice.

228 espectively); both also express pumps with a ouabain-resistant alpha1 subunit.

229 opus alpha1beta3 Na/K pumps, which were made ouabain-resistant by point mutation, after expressing th

230 nfusion induces hypertension in rodents, and ouabain-resistant mutation of the alpha2 ouabain binding

231 Na/K pumps from ventricular myocytes and in ouabain-resistant pumps expressed in Xenopus oocytes.

232 -induced defect was rescued by expression of ouabain-resistant zebrafish alpha1a.1 or rat alpha1 mRNA

233 lpha1 is ouabain-sensitive and NKA-alpha2 is ouabain-resistant, we assessed the effects of PLM phosph

234 IR channels and Na(+)/K(+)-ATPase (BaCl2 and ouabain, respectively), and combined inhibition of KIR c

235 low concentration of the cardiac glycoside, ouabain, resulted in a modest increase in intracellular

236 Furthermore, in vivo, administration of ouabain reversed the imbalance between Bax and Bcl-xL in

237 nduces hypertension, and digoxin antagonizes ouabain's hypertensinogenic effect.

238 mitochondrial depolarization observed before ouabain-SD was abolished by L-type channel block, and Zn

239 ) and Zn(2+) removal was required to prevent ouabain-SD when A(1) receptors were blocked.

240 ncreases were observed in CA1 neurons before ouabain-SD, and L-type channel block prevented the intra

241 oval of extracellular Ca(2+) did not prevent ouabain-SD.

242 an play a critical role in the generation of ouabain-SD.

243 e into susceptible eucaryotic cells under an ouabain selection regime.

244 diotonic steroids (CTSs) such as digoxin and ouabain selectively inhibit Na+, K+ -ATPase (the Na+ pum

245 In all cases, the ouabain sensitive component of the influx contributed ap

246 es Na(+),K(+)-ATPase activity as assessed by ouabain-sensitive (86)Rb(+) uptake.

247 fragment, the sodium excretion rates in the ouabain-sensitive alpha1 isoform mice were equivalent to

248 Circulating EO modulates ouabain-sensitive alpha2 Na(+) pump activity and Ca(2+)

249 Elevated hydrostatic pressure induced ouabain-sensitive alveolar fluid secretion that coincide

250 wild-type mice and mice where NKA-alpha1 is ouabain-sensitive and NKA-alpha2 is ouabain-resistant, w

251 Measurement of ouabain-sensitive ATPase activity and radiolabeled catio

252 sgenic mice also exhibited a 36% decrease in ouabain-sensitive sodium reabsorption and a significantl

253 pNBTEA concentration and V(M) dependence of ouabain-sensitive transient charge movements in whole-ce

254 ep changes in V(M) elicited pNBTEA-activated ouabain-sensitive transient currents that had similariti

255 ximately 60 s), activity-dependent, TTX- and ouabain-sensitive, hyperpolarization ( approximately 5 m

256 Endogenous, ouabain-sensitive, Xenopus alpha1beta3 Na/K pumps were s

257 nsistent with the established differences in ouabain sensitivity between pig and rat alpha1.

258 Currently, the basis for the differences in ouabain sensitivity of NHK and ADPKD cells is unknown.

259 by micropuncture in mutant mice with altered ouabain sensitivity of the alpha1 and alpha2 Na,K-ATPase

260 of the alpha2 isoform to ouabain, conferring ouabain sensitivity to alpha1 augmented the natriuretic

261 d cardiac arrhythmia and heart failure drug, ouabain, shows potential antitumor activities in lung ad

262 Cardiotonic steroids (such as ouabain) signaling through Na/K-ATPase regulate sodium r

263 Values for preoperative endogenous ouabain significantly improved (area under curve: 0.85)

264 t was found that nanomolar concentrations of ouabain, similar to those circulating in blood, induced

265 Furthermore, ouabain stimulated direct carbonylation of two amino aci

266 Ouabain-stimulated Na(+),K(+)-ATPase signaling has recen

267 he antioxidant N-acetyl-L-cysteine prevented ouabain-stimulated Na/K-ATPase.c-Src signaling, protein

268 tive oxygen species are required to initiate ouabain-stimulated Na/K-ATPase.c-Src signaling.

269 Ouabain-stimulated protein carbonylation is reversed aft

270 /K-ATPase.c-Src signaling complex attenuated ouabain-stimulated protein carbonylation.

271 It is concluded that ouabain stimulates proliferation in ADPKD cells by bindi

272 However, ouabain stimulates the PI3K/Akt/mTOR pathway and consequ

273 mparison with the low-affinity [K2]E2-MgF(x)-ouabain structure shows that the CTS binding pocket of [

274 ith each incremental preoperative endogenous ouabain tertile.

275 ) increased with the preoperative endogenous ouabain tertile.

276 by bath application of low concentrations of ouabain that selectively inhibit NKAalpha3 while alpha-m

277 In the presence of ouabain, the action potential collapses.

278 In constructs exposed to ouabain, the increased percentage of collagen per constr

279 At 1 mumol l(-1), ouabain, the secreted saliva was hyperosmotic.

280 Src kinase and inhibit its activity, whereas ouabain, the specific Na,K-ATPase inhibitor, binds and s

281 c activity but also serves as a receptor for ouabain to activate protein kinases.

282 nses of the alpha1 and/or alpha2 isoforms to ouabain to assess for altered natriuretic responses to a

283 wn converts the growth stimulatory effect of ouabain to growth inhibition in LLC-PK1 cells.

284 t-shock protein 27 (HSP27) was necessary for ouabain to inhibit HIF-1alpha translation.

285 Combined infusion of ouabain (to inhibit Na(+)/K(+)-ATPase) and barium chlori

286 a receptor for cardiotonic steroids, such as ouabain, to regulate cellular protein kinase cascades.

287 Control (PLM(WT)), transgenic (PLM(3SA)), ouabain-treated and hypertrophied Langendorff-perfused m

288 and activation of ERK1/2 in the striatum of ouabain-treated mice.

289 ) current was fully inhibited by basolateral ouabain treatment, suggesting that the Na(+),K(+)-ATPase

290 ression analysis, the circulating endogenous ouabain value before surgery was the strongest predictor

291 ffected differently by Na(+) modulation with ouabain versus Ca(2+) modulation with ionomycin.

292 Surprisingly, ouabain virtually abolished NKA-PLM FRET but only partia

293 In addition, ouabain was able to stimulate Src and ERK1/2 in the resc

294 Preoperative endogenous ouabain was measured in 407 patients admitted for electi

295 nergy transfer distances measured with bound ouabain, we carried out molecular dynamics simulations w

296 Low concentrations of ouabain were also found to disrupt cortical network osci

297 Dopamine/ouabain were not involved in activation of protein secre

298 ternalization of alpha1 induced by >/=100 nm ouabain which occurs in a time scale of hours.

299 were subjected to intravitreal injections of ouabain, which destroys the inner retina.

300 diac glycosides (CGs) digoxin, digitoxin and ouabain, which directly inhibit ATP1A1 function, exhibit