ライフサイエンスコーパス: outside-out

1 levated NP(o) of unitary calcium currents in outside-out and cell-attached patches, and elevated calc

2 ion of caffeine or noradrenaline and in both outside-out and inside-out patches when the internal pat

3 ique (cell-attached, inside-out, whole-cell, outside-out and perforated patch) can be achieved, even

4 ced Cl- current (IGABA) was quantified using outside-out and whole-cell patch-clamp recordings beginn

5 r configurations (cell-attached, inside-out, outside-out, and whole-cell).

6 used to test this hypothesis in whole-cell, outside-out, cell-attached, and inside-out patches from

7 on (DRG) neurons were investigated using the outside-out configuration of patch-clamp technique.

8 Using the outside-out configuration of the patch clamp, we elicite

9 d the patch clamp recording technique in the outside-out configuration to investigate, at the single

10 Whole-cell and outside-out configurations of the patch clamp technique

11 icate those at the native synapse, recording outside-out currents elicited by fast application of mil

12 icate those at the native synapse, recording outside-out currents elicited by fast application of mil

13 oxinin and picrotin inhibited glycine-evoked outside-out currents, picrotin had a 30 times higher IC5

14 Dual somato-dendritic recording, outside-out dendritic recordings, and focal application

15 Using whole-cell patch-clamp and outside-out excised patch configurations, we found that

16 eceptors expressed in Xenopus oocytes, using outside-out excised patch recording.

17 skin barrier keeps the 'inside in' and the 'outside out', forming a protective blanket against exter

18 ely dissociated preparation and a perforated outside-out macropatch of dendritic membrane.

19 LTD, produced in a perforated outside-out macropatch of Purkinje neuron dendrite, whic

20 R334C-CFTR channels in outside-out macropatches activated by ATP alone were mod

21 ent outward potassium current was studied in outside-out macropatches excised from the soma of CA1 py

22 Shaker K+ channels were expressed in outside-out macropatches excised from Xenopus oocytes, a

23 roM) in the presence of 10 microM glycine to outside-out macropatches resulted in openings with an av

24 the modification of R334C-CFTR, measured in outside-out macropatches using a rapid perfusion system,

25 and perforated patch whole-cell and excised outside-out membrane patch recording configurations.

26 ans of a piezoelectric translator to excised outside-out membrane patches allows concentration jumps

27 Brief (2-3 ms) applications of 1 mM GABA to outside-out membrane patches containing alpha1beta3, alp

28 ethyl-lactam potentiates currents in excised outside-out membrane patches elicited by the prolonged a

29 ents were resolved by pulses of glutamate to outside-out membrane patches from Purkinje cells.

30 n of BK-type Ca(2+)-activated K+ channels in outside-out membrane patches from rat olfactory bulb gra

31 Single-channel recordings were made from outside-out membrane patches of Xenopus oocytes injected

32 In outside-out membrane patches, co-application of ACh (4 m

33 In excised outside-out membrane patches, the effects of light on NM

34 brief pulses (1-10 ms) of 1 mM glutamate to outside-out membrane patches, we observed a low-conducta

35 Using excised outside-out membrane patches, we studied the mechanism b

36 duced by application of glutamate or NMDA to outside-out membrane patches.

37 lower when the drug is applied externally to outside-out membrane patches.

38 potency, unitary I(AC) currents recorded in outside-out membrane patches.

39 f single-channel and macroscopic currents in outside-out membrane patches.

40 be resolved in response to ATP (3 microM) in outside-out membrane patches.

41 robability of Kv channel opening in excised, outside-out membrane patches.

42 evoked by brief applications of glutamate to outside-out membrane patches.

43 Channels in patches excised in outside-out mode were blocked by 1 mM Ba2+ but were unaf

44 ell and a granule cell in the whole cell and outside-out modes, respectively, depolarizations of Berg

45 otein in proteoliposomes containing hSMVT in outside-out orientation yielded a catalytic turnover num

46 Second, simultaneous outside-out patch and whole-cell recordings reveal that

47 Outside-out patch clamp recording revealed that dendriti

48 We used current clamp and outside-out patch clamp recording to investigate dendrit

49 hole-cell recordings from HEK 293 cells, and outside-out patch clamp recordings from both cell types.

50 Outside-out patch clamp recordings revealed that the max

51 um currents, recorded in both whole-cell and outside-out patch configurations, demonstrated a selecti

52 fluorescent protein in growing axons, and an outside-out patch from mature neuronal membranes that co

53 these channels was assessed in whole-cell or outside-out patch recording as the degree of inward rect

54 We used outside-out patch recording of Xenopus alpha1beta3 Na/K

55 Outside-out patch recordings confirmed this segregation.

56 Outside-out patch recordings demonstrated that the ampli

57 Outside-out patch recordings from cerebellar Purkinje ce

58 f reduced-current, multi-channel bursting in outside-out patch recordings from hnAChRs expressed in t

59 binding (the first latency) was estimated in outside-out patch recordings from rat hippocampal neuron

60 Whole-cell recordings and outside-out patch recordings from TE671 cells were made

61 Whole-cell and outside-out patch recordings in slices from bacterial ar

62 By combining outside-out patch recordings of BG AMPA receptors and qu

63 Outside-out patch recordings revealed identical unitary

64 ependent potassium current was studied using outside-out patch recordings with rapid application of d

65 application of GABA + 3alpha5alphaP, and in outside-out patch recordings, suggesting that steroid di

66 its and the kinetic properties examined with outside-out patch recordings.

67 of how fast solutions can be exchanged on an outside-out patch using a dual stream switcher.

68 Following tetanic stimulation an outside-out patch was excised from the apical dendrite n

69 s in response to ATP (whole cell and excised outside-out patch) showed that all formed functional cha

70 ucleus (MNV) of the rat using whole-cell and outside-out patch-clamp recording in coronal brainstem s

71 Using whole-cell and outside-out patch-clamp recordings from granule cells in

72 postnatal development, using whole-cell and outside-out patch-clamp recordings in acute cerebellar s

73 Instead, using outside-out patch-clamp recordings, we demonstrate that

74 is and detected by the use of whole-cell and outside-out patch-clamp techniques on freshly dissociate

75 Here, using outside-out, patch clamp recordings and fast ligand appl

76 Moreover, the consistent blockade seen in outside out patches might be ascribed to the confinement

77 and concentration jump techniques applied to outside out patches to evaluate the impact of these muta

78 gonist concentration jumps were performed on outside out patches with multiple NR1/NR2C channels, whi

79 When applied to GABA(A) receptor traces (outside out patches, alpha 1 beta 2 gamma 2S, 1 mM GABA,

80 was applied with a piezoelectric stepper to outside out patches, to simulate its fast rise and short

81 receptor response to 100 microM serotonin in outside-out patches (n = 19) and whole cells (n = 41) de

82 modelling of currents from cell-attached and outside-out patches (where the number of channels in the

83 y 3 ms, 30 mM) applied to mutant channels in outside-out patches activated currents with a slower ris

84 excitatory postsynaptic conductance, we used outside-out patches and a fast application system to cha

85 single GABA channels from cell-attached and outside-out patches and also introduced some of the prel

86 pionic acid (AMPA) receptors was examined in outside-out patches and at glutamatergic synapses in neu

87 Using outside-out patches and fast ligand application, we exam

88 ophysiological measurements on nucleated and outside-out patches and in the whole-cell mode also yiel

89 ificantly reduced the A-type K(+) current in outside-out patches and nearly eliminated the distance-d

90 his agent was applied externally to cells or outside-out patches at concentrations of 5 to 10 microM.

91 diated currents were also studied in somatic outside-out patches at P13-P15 with fast application of

92 ition of acetylcholine-activated currents in outside-out patches by (+)-tubocurarine, pancuronium and

93 ng, we recorded single-channel currents from outside-out patches containing a single active NR1/NR2C

94 Applying glutamate to outside-out patches containing a single NMDAR, we find t

95 apidity to mediate prejunctional depression, outside-out patches containing both ATP-gated and ACh-ga

96 Rapid applications of glutamate on outside-out patches containing NR1a/NR2D(T692A) receptor

97 s studied using ultra-fast drug perfusion of outside-out patches containing rat GluR-A or GluR6 subun

98 In isolated outside-out patches CPA did not evoke SOCs but noradrena

99 Recordings from outside-out patches demonstrated that inward rectificati

100 Outside-out patches displayed two distinct patterns of s

101 econd applications of 1 mM GABA to nucleated outside-out patches elicited rapidly rising biexponentia

102 Fast application of GABA (10 mM) onto outside-out patches excised from bipolar cell terminals

103 Outside-out patches excised from cells transfected with

104 Outside-out patches excised from epsilon subunit-contain

105 applications of GABA (1 mM) to nucleated and outside-out patches excised from granule neurons in cere

106 Single-channel activities were observed in outside-out patches excised from oocytes expressing a ma

107 by using rapid applications of glutamate to outside-out patches excised from Purkinje neurons.

108 (A) receptor antagonist-sensitive current in outside-out patches excised from RGCs, indicating that a

109 NMDA currents recorded from outside-out patches excised from the distal dendrites of

110 In outside-out patches excised from the nonsynaptic face of

111 d using rapid applications of L-glutamate to outside-out patches excised from transfected human embry

112 nel number times open probability (nP(o)) in outside-out patches excised from Xenopus oocytes, with n

113 by their excitatory transmitter, we examined outside-out patches exposed to glutamate.

114 Using outside-out patches formed <or=250 microm away from the

115 educed gamma of 5-HT(3A)(R436C) receptors in outside-out patches from 7.8 +/- 0.5 to 5.0 +/- 0.5 pico

116 brief (1 ms) application of 5-10 mm GABA to outside-out patches from acutely isolated CA1 hippocampa

117 Transporter currents in outside-out patches from astrocytes have faster kinetics

118 perties and distributions of ion channels in outside-out patches from axons and somata of layer 5 pyr

119 tors expressed in Xenopus laevis oocytes and outside-out patches from BOSC 23 cells.

120 Outside-out patches from both cell types under both trea

121 ACh receptors (nAChRs) were investigated in outside-out patches from CA1 stratum radiatum interneuro

122 We find that application of L-glutamate to outside-out patches from cerebellar Bergmann glia activa

123 In outside-out patches from CG neurones containing a 38 pS

124 In outside-out patches from CG neurones, the K+ channel wit

125 Rapid application of 10-300 microM NMDA to outside-out patches from cultured cortical neurons evoke

126 functional binding sites on AMPA channels on outside-out patches from cultured hippocampal neurons.

127 NMDA-activated patch current were studied in outside-out patches from cultured rat cortical neurons.

128 Outside-out patches from Golgi cells exhibited a populat

129 ysed using concentration-jump techniques and outside-out patches from HEK 293 cells.

130 d also activated TRPV4 currents in cell-free outside-out patches from HEK293T cells overexpressing TR

131 Here, in outside-out patches from heterologous cells stably expre

132 es of P2X ATP receptors were investigated in outside-out patches from hippocampal granule cells in br

133 ity of expressed alpha1beta1gamma2 GABARs in outside-out patches from human embryonic kidney 293 cell

134 e to the rapid application of l-glutamate to outside-out patches from human embryonic kidney cells ex

135 Furthermore, D-aspartate-induced currents in outside-out patches from IPCs exhibited larger steady-st

136 ed under hyposmolar recording conditions for outside-out patches from L/M interneurons; no changes we

137 Voltage clamp of outside-out patches from L2/3 neurons revealed that the

138 rations, the open probability of channels in outside-out patches from migrating cells was very high,

139 and kainate receptors show this behavior in outside-out patches from neurons in situ by measuring co

140 Voltage-clamp recordings from outside-out patches from NTS neurones revealed an outwar

141 annel kainate-type currents were observed in outside-out patches from proliferating granule cells in

142 we recorded TTX-sensitive sodium currents in outside-out patches from Purkinje cells acutely isolated

143 Two inactivating currents were present in outside-out patches from pyramidal cells: a rapidly inac

144 ansporter currents were also not detected in outside-out patches from pyramidal neurons.

145 tive cation channel currents were studied in outside-out patches from rabbit portal vein smooth muscl

146 ated channels (10 +/- 1 pS) in inside-out or outside-out patches from rat cultured hippocampal neuron

147 ock of NMDA receptors was studied in excised outside-out patches from rat hippocampal neurons and Xen

148 GABA(A) receptor agonists and antagonists in outside-out patches from rat hippocampal neurons.

149 d, anion-potentiated transporter currents in outside-out patches from these cells exhibited larger am

150 We rapidly applied acetylcholine to outside-out patches from transfected BOSC23 cells and me

151 y 80% of the mechanically induced current of outside-out patches from transfected HEK293 cells.

152 ) glutamate receptor channels was studied in outside-out patches from transiently transfected HEK 293

153 Rs that gave inwardly rectifying currents in outside-out patches from TTX-treated cells was six times

154 iflumic acid (NFA) in excised inside-out and outside-out patches from Xenopus oocytes.

155 Outside-out patches gave a much higher success rate, but

156 ntaneous single-channel events recorded from outside-out patches had the same chord conductance as GA

157 In whole-cell and somatic outside-out patches I(A) activated at -60 mV (half-activ

158 by rapid applications of GABA onto nucleated outside-out patches in cultured postnatal rat hippocampa

159 With isolated outside-out patches Ins(1,4,5)P3 markedly increased the

160 Pulses of ACh were applied to outside-out patches of cell membrane by means of a fast

161 In single-channel recordings from excised outside-out patches of cortical neurons, ethanol inhibit

162 methyl-D-aspartate (NMDA) were recorded from outside-out patches of cultured rat cortical neurons in

163 Agonist pulses applied to outside-out patches of cultured rat hippocampal neurons

164 BA-activated ion channels were recorded from outside-out patches of granule cell bodies.

165 es P(OPEN) in single-channel recordings from outside-out patches of HEK 293 cells.

166 nown superfamilies of NGICs in fast-perfused outside-out patches of membrane.

167 Using outside-out patches of mPFC pyramidal neurons, which pre

168 Outside-out patches pulled from PKD1(115-226)-expressing

169 with voltage clamp, we applied glutamate to outside-out patches pulled from transiently transfected

170 Focally applied GABA and glycine onto outside-out patches revealed that the GABAergic to glyci

171 High-resolution recordings from outside-out patches showed that in all Kir2 channels cur

172 ps (1 ms) of 10 mM glutamate were applied to outside-out patches so that a comparison between the mac

173 In about 45 % of isolated outside-out patches spontaneous channel currents with a

174 Recordings from outside-out patches that contain a single active channel

175 n techniques were used to apply glutamate to outside-out patches that contained GluK1, GluK1/5, or Gl

176 e of open and closed durations recorded from outside-out patches that contained one active NR1/NR2C c

177 Here we report data from outside-out patches that indicate GluR1-containing recep

178 In outside-out patches there was spontaneous cation channel

179 NPo) of the NMDA receptor channel in excised outside-out patches was attenuated by 3HPG but not by 4C

180 Outside-out patches were equilibrated with cisatracurium

181 brief, quasi-synaptic pulses of glycine onto outside-out patches were impaired in mutant receptors, a

182 l currents caused by ACh and ATP in the same outside-out patches were less than additive (85 +/- 10 %

183 Channels in excised outside-out patches were modulated by ligand, indicating

184 -like millisecond applications of glycine to outside-out patches were much shorter (7- to 10-fold) in

185 cations of GABA (from 10 microM to 10 mM) to outside-out patches were used to study the role that the

186 e ASICs show extremely rapid deactivation in outside-out patches when jumping from a pH 5 stimulus to

187 In outside-out patches with 250 mM external NaCl, the singl

188 2+ inhibited spontaneous channel activity in outside-out patches with IC50 values of 6.8 microm, 25 n

189 inhibited single-channel currents in excised outside-out patches without significantly changing mean

190 in wild-type mouse cells (observed in 50% of outside-out patches), but never observed in mdx cells.

191 d beta-BuTX, unitary currents were recorded (outside-out patches, -60 or -50 mV) that were smaller th

192 In outside-out patches, 11,12-EET also increased the KCa ch

193 With outside-out patches, a transient early suppression of th

194 used membrane breakdown; however, in excised outside-out patches, Ag NPs activated Gd(3+) -sensitive

195 annel open probability determined in excised outside-out patches, but has no effect on single-channel

196 In both inside-out and outside-out patches, channel activity was depressed and

197 the off rate was nearly identical to that of outside-out patches, differences were observed for the o

198 In outside-out patches, ethylbromide tamoxifen increased BK

199 In outside-out patches, GTP was present in the pipette and

200 In outside-out patches, just as desensitized states prolong

201 In outside-out patches, nicotine activated brief 60- and 80

202 In nucleated outside-out patches, PS depressed peak currents and spee

203 In outside-out patches, QA ions in which ethyl groups of TE

204 In excised outside-out patches, the addition of dibutyryl cAMP cons

205 In outside-out patches, the TASK-like background K+ channel

206 In outside-out patches, the type I channel had an almost li

207 In patch-clamp experiments with outside-out patches, THIK-1 currents were also inhibited

208 Under voltage-clamp conditions in outside-out patches, this crosslink was reduced by DTT a

209 In outside-out patches, troglitazone inhibited KATP channel

210 recordings were made of calcium channels in outside-out patches, under conditions which favoured the

211 In outside-out patches, unedited homomeric GluR6(Q) recepto

212 Additionally, in patch-clamp recording from outside-out patches, we found that oligomerized aSyn can

213 channel openings in any capsaicin-sensitive outside-out patches.

214 microcystin also stimulated SOCs in isolated outside-out patches.

215 perties of the 5-HT(3)R were investigated in outside-out patches.

216 ck by Mg(2+)(i) similar in cell-attached and outside-out patches.

217 examined using cell-attached, inside-out and outside-out patches.

218 steady-state currents from cell-attached and outside-out patches.

219 aleimide, and could be elicited in cell-free outside-out patches.

220 homomeric GluR1(flip) receptors recorded in outside-out patches.

221 annel activity of Kir2.3 currents in excised outside-out patches.

222 1 ms pulses of high concentration glycine to outside-out patches.

223 cts on IPSCs with GABAA channel responses in outside-out patches.

224 ATP activated single channel currents in outside-out patches.

225 on GluN1/GluN2A channels studied in excised outside-out patches.

226 on adherent cells, lifted cells, or excised outside-out patches.

227 currents of the mechanical channel TREK-1 on outside-out patches.

228 nels and 15-45 pS TRPC6 channels in the same outside-out patches.

229 nm Ang II induced TRPC6 channel activity in outside-out patches.

230 hannel activity evoked by 1-100 nm Ang II in outside-out patches.

231 tenuated I(A) in both whole-cell and somatic outside-out patches.

232 ls or (ii) piezo-driven solution exchange in outside-out patches.

233 nts elicited by rapid glycine application to outside-out patches.

234 patches but failed to do so in inside-out or outside-out patches.

235 pulses, and high-frequency trains of GABA to outside-out patches.

236 ties of individual NMDA receptor channels in outside-out patches.

237 ution exchange to study receptor kinetics in outside-out patches.

238 , when 5 microm ruthenium red was applied to outside-out patches.

239 que in cell-attached and excised (inside-out/outside-out) patches from embryonic rat dorsal root gang

240 ugh single channels were studied in excised (outside-out) patches.

241 MDARs and alpha7 nAChRs, and recordings from outside-out pulled patches of enlarged presynaptic bouto

242 rent-clamp somatic and dendritic recordings, outside-out recordings of dendritic sodium and potassium

243 channels was investigated in parallel using outside-out single channel recording.

244 Outside-out sniffer patches pulled from neurons in the a