ライフサイエンスコーパス: pH dependence

1 y residues in other proteins causing similar pH dependence.

2 electron transfer as well as changes in its pH dependence.

3 the urease reaction with a bell-shaped rate-pH dependence.

4 to those of the native pigment exhibiting no pH dependence.

5 stimulation, and (as revealed in this study) pH dependence.

6 parable to those of the wt in efficiency and pH dependence.

7 actions at this position and/or change their pH dependence.

8 M is dominated by one conformation with weak pH dependence.

9 favored at lower pH, whereas the DM shows no pH dependence.

10 c activity, substrate affinity, and reaction pH dependence.

11 t qualitatively agrees with the experimental pH dependence.

12 periments revealed three distinct regions of pH dependence.

13 ted mutagenesis indeed eliminated the strong pH dependence.

14 147) to Val, which had minimal effect on the pH dependence.

15 onohydrogen-phosphate, as can be seen by the pH dependence.

16 approximately 500-fold, with maintenance of pH dependence.

17 oxidation of phen by PMS features a complex pH dependence.

18 however, His378Asn/Arg variants show no such pH dependence.

19 rption capacity, which demonstrated the same pH dependence.

20 results of metal alteration, mutations, and pH dependence.

21 arrier for the rate-determining step and its pH dependence.

22 xhibiting greater longevity and a pronounced pH-dependence.

23 s of the wild-type and mutant VCPO and their pH-dependence.

24 experiments were small and showed no notable pH-dependence.

25 ere incorporated into a kinetic model of the pH dependence and compared to a pseudo-steady-state mode

26 Pore formation has a very steep pH dependence and is also dependent on peptide concentra

27 dues are likely the structural basis for the pH dependence and sensitivity to ionic strength of influ

28 The pH dependence and thermostability reveal that the enzyme

29 id residues suggested that the face-specific pH dependences and Na(+)/K(+) selectivities may arise fr

30 es, an increased affinity for ATP, different pH dependence, and a decreased sensitivity to azide inhi

31 ility of ZIP8, its subcellular localization, pH dependence, and regulation by iron.

32 On the basis of kinetic properties, pH dependence, and structural information, we propose an

33 This observed pH-dependence appears to result from photooxidants react

34 stabilization was consistent with the strong pH dependence arising from the GKD tripeptide unit.

35 His51 cannot account for the observed pH dependence as neutral amino acid substitutions failed

36 exhibit the same kinetic behaviors (KIE and pH dependence) as the catalyzed chemical reaction.

37 The pH dependence, as determined from the primary isotope ef

38 The midpoint potential displays a distinct pH dependence at acidic pH values, indicative of proton-

39 a I(480) decay did not display the anomalous pH dependence characteristic of classical Meta II in the

40 (I) affinity of WT HAH1 exhibits a different pH-dependence compared to MNK1 and Lys60Ala HAH1.

41 rotein stability, the hydrogen bond network, pH dependence, conformational dynamics and protein funct

42 mutated to histidine, the enzyme develops a pH dependence corresponding to a loss of catalytic power

43 nd equilibrium experiments suggests that the pH dependences determining membrane association and tran

44 ble anodic photocurrent whose potential- and pH-dependences exhibit broad applicability.

45 The lack of pH dependence explains in part why NP4 cannot use the fe

46 hat the differences in both Tafel slopes and pH dependence for C(1) vs C(2) activity arise from diffe

47 ve distinct trafficking routes, we suggest a pH dependence for multiple cargo sorting events at the G

48 his hypothesis is examined in the context of pH dependence for other members of the Kv1 family, and m

49 revealed the structural determinants of the pH dependence for the interaction of these inhibitors wi

50 The disparate pH dependences for reactivation of ChE and the general b

51 for trace Tl adsorption indicated a moderate pH-dependence from pH 2.5 to 11.

52 This pH dependence has been one of the major disadvantages th

53 The observed speed-pH dependence holds promise for sensitive pH measurement

54 The pH dependence, hydrogen/deuterium (H/D) isotope effect,

55 Steady-state kinetics exhibited a pH dependence in k(cat), which prompted us to elucidate

56 ion shifts at multiple sites, increasing the pH dependence in the mutant.

57 The FHA-FBM interactions exhibit significant pH dependence in the physiological range as a consequenc

58 acid (DMAPA, azinoyl = N(+)(O(-)) has a weak pH-dependence in D(2)O, with a slight preference for tra

59 There was a pH-dependence in the reduction of NO2(-) by magnetite; t

60 th a rate constant that showed a bell-shaped pH dependence indicative of participation of factor Xa a

61 onic stress in erythrocyte membranes had the pH dependence, ion dependence, and inhibitor sensitivity

62 gher pH, the functional significance of this pH dependence is unknown.

63 At lower pH < 7.5, the pH dependence is weak and shows a previously measured KI

64 The absorption spectrum also exhibits a pH dependence larger than any other retinal protein.

65 , dark background, facile synthesis, and low pH dependence make NO(550) a superior probe for NO detec

66 n-hydroxyl co-adsorption causes the apparent pH dependence of "hydrogen" adsorption in the step sites

67 Although we earlier had observed the pH dependence of a (15)N NMR signal from each of the two

68 The Mn(II) concentration and pH dependence of a preceding lag phase indicates weak Mn

69 GMQ shifts the pH dependence of activation to more acidic pH in ASIC1a

70 ombination of the ionic-strength effect, the pH dependence of anion adsorption, and the competition b

71 Analyses of the pH dependence of ApbE activity indicate that the pK(a) o

72 a surface complexation model describing the pH dependence of As(III) binding to the organic adsorben

73 The 5b molecule not only shifts pH dependence of ASIC1a activation but also inhibits its

74 The pH dependence of assembly kinetics and extent of assembl

75 Analysis of the pH dependence of assigned (13)C', (13)C(alpha), and (15)

76 The pH dependence of binding had an apparent pKa of 7.2, a v

77 The pH dependence of binding reveals a pK(a) of 6.7, suggest

78 The pH dependence of binding, obtained by nuclear magnetic r

79 model for the zebrafish embryo explained the pH dependence of biouptake and toxicity.

80 A comparison of the pH dependence of both kred and kred/Kd from reductive ha

81 The impact of AdoMet binding and the pH dependence of catalysis are also quantitatively obser

82 The pH dependence of catalysis, the turnover frequency, and

83 Combination of acidity measurements with the pH dependence of catalytic rates unequivocally shows tha

84 The pH dependence of cell-expressed mutants (L1C and L2 swap

85 tral binding site) reduced also the internal pH dependence of ClC-5.

86 asparagines exhibits a much less pronounced pH dependence of collagen binding.

87 It is known that the pH dependence of conductance for the rat potassium chann

88 e of Molecular Microbiology, analyses of the pH dependence of cytoplasmic [Na(+)], [K(+)], pH and tra

89 We were also able to model the pH dependence of diffraction of the HEMA PCCA by using F

90 The pH dependence of digestion patterns shows that, in the p

91 hFc with the capture surface, influences the pH dependence of dissociation from the capture surface.

92 The pH dependence of enzyme activity and inhibition has been

93 , these variants showed a markedly different pH dependence of fibril formation versus that of PAPf39.

94 ough apparent pKa values determined from the pH dependence of fluorescence intensity shift in the bas

95 onomeric and characterized by the pronounced pH dependence of fluorescence, relatively high brightnes

96 H170 interaction in dimer stability and the pH dependence of fusion and provide evidence for stepwis

97 studies, mass spectrometry studies, and the pH dependence of guest encapsulation.

98 Alternative explanations for the pH dependence of hairpin ribozyme reactivity are discuss

99 -Changeux allosteric model revealed that the pH dependence of Hb-O(2) affinity stems primarily from c

100 take is more flexible as it accounts for the pH dependence of HONO uptake and bulk diffusion in the s

101 zation mass spectrometry (CIMS) to study the pH dependence of HONO uptake onto agricultural soil and

102 also present an initial investigation of the pH dependence of hPRL function in rat Nb2 cell prolifera

103 GMQ also induces an acidic shift of the pH dependence of inactivation of ASIC1a, -1b, -2a, and -

104 The pH dependence of inhibition (K(ii)), on the contrary, in

105 state and pre-steady-state kinetics, and the pH dependence of inhibition and binding.

106 ions tested; however, the measurement of the pH dependence of iodoacetamide alkylation revealed a pK

107 f ICRAC on intracellular pH strictly follows pH dependence of iPLA2beta activity, and 7) (S)-BEL, a c

108 Consistent with the pH dependence of iron binding, the main trigger for iron

109 By analyzing the pH dependence of isomerization and protonation equilibri

110 he pKa of 2 was estimated to be 6.9 from the pH dependence of its ring-closing to the pyrimidopurinon

111 operties of hPRL, we have noted a surprising pH dependence of its structural stability over a range f

112 The pH dependence of IYD binding and turnover also supports

113 The pH dependence of k cat for E155A in the presence of form

114 d 1.8 on V/ K AcCoA and V, respectively; the pH dependence of k cat was similar to that of the wt.

115 In contrast, the pH dependence of k(cat) values indicates a much lower ap

116 For the manganese substituted BcII, the pH dependence of k(cat)/K(m) for benzylpenicillin, cepha

117 The pH dependence of Kb revealed that the RA carboxylic form

118 The pH dependence of kcat/Km and of kcat for the wild-type P

119 The pH dependence of KCC in "euvolemic" SS RBCs treated with

120 euterium isotope effects, and changes in the pH dependence of kinetic parameters compared to that of

121 The pH dependence of kinetic parameters suggests that the hy

122 sm for HIcDH is proposed on the basis of the pH dependence of kinetic parameters, dissociation consta

123 al mechanism is proposed on the basis of the pH dependence of kinetic parameters, dissociation consta

124 Analysis of the pH dependence of L-glutamate stereoisomerization suggest

125 Kinetic analyses show a pH dependence of ligand binding that is consistent with

126 The pH dependence of LMCT-mediated AFO photoreductive dissol

127 The pH dependence of lysozyme activity arises not from chang

128 This pH dependence of metal ion binding suggests that the loc

129 The pH dependence of metalation under physiological conditio

130 From the pH dependence of methyl chemical shifts, a pKa of 7.7 is

131 ersion, we have measured the temperature and pH dependence of microsecond to millisecond time scale b

132 a tautomeric or ionized base pair, with the pH dependence of misinsertion consistent with the latter

133 more His residues (pK(a) ~6.0) mediates the pH dependence of multimerization.

134 found to be responsible for controlling the pH dependence of NAADP synthesis by the cyclase.

135 anism by which these mutations influence the pH dependence of NhaA, the substrate dependence of the k

136 Finally, we could measure the pH dependence of NPQ, showing that the luminal pH is alw

137 are not important factors in controlling the pH dependence of O(2)(*-)-mediated AFO dissolution.

138 A conceptual model describing the pH dependence of PAPf39 aggregation is proposed and prov

139 ssary for fibril formation but modulates the pH dependence of PAPf39 fibril formation.

140 To do so we took advantage of the pH dependence of parallel Hoogsteen interactions and rat

141 The bell-shaped pH dependence of permeability suggests that, in addition

142 The single turnover pH dependence of pre-tRNA cleavage revealed a single ion

143 length PA83 protein in the pH 5-6 range, the pH dependence of prepore-to-pore conversion of (PA63)7 w

144 Modeling the pH dependence of protein and peptide chemical shifts out

145 The pH dependence of protein stability calculated from NMR-d

146 mine the unfolded state pK(a) values and the pH dependence of protein stability for this enzyme.

147 Analysis of the pH dependence of quasi-reversible features in cyclic vol

148 The trend of pH dependence of rates for Trp (i.e., aromatic alpha-ami

149 The pH dependence of reduction was also determined, and a pK

150 tion of the conserved His(151) abolished the pH dependence of RIPK1 activation, suggesting that this

151 The pH dependence of seHAS in the presence of synthetic or n

152 The pH dependence of silicate sorption exhibited a maximum b

153 The model calculations reveal a strong pH dependence of solubility, typically controlled by the

154 The mutants D302A and H305A remove the pH dependence of stability.

155 ate-inhibited, but unlike the H287C variant, pH dependence of substrate inhibition was largely elimin

156 the site of protonation responsible for the pH dependence of sugar binding.

157 The pH dependence of superoxide-mediated Fe(III) reduction (

158 A breakpoint in the pH dependence of the 2'-O-transphosphorylation rate to a

159 We have measured and analyzed the pH dependence of the 695 nm charge transfer band of hors

160 data by QM/MM calculations suggest that the pH dependence of the binding of NO, but not of CN(-) and

161 The pH dependence of the catalytic activity also implicates

162 site-directed mutagenesis, evaluation of the pH dependence of the catalytic efficiency (V(max)/K(M)),

163 in the pili, as well as the temperature and pH dependence of the charge density, were similar to tho

164 e and found a strong correlation between the pH dependence of the cleavage reaction and the intrinsic

165 The pH dependence of the competitive inhibition constant, K(

166 Based upon the observed pH dependence of the current amplitude and oxidation/red

167 studied the external and internal Cl(-) and pH dependence of the currents of E268A.

168 substrates was investigated by measuring the pH dependence of the D(kcat/KNE) value with 1,1-[2H2]-ni

169 recognition helix accounted for most of the pH dependence of the DNA binding.

170 sfer processes and can be interpreted by the pH dependence of the emitted light.

171 e binding and catalysis without changing the pH dependence of the enzyme.

172 te similar to that obtained by analyzing the pH dependence of the epsilon(240) of wild-type AhpC (5.8

173 The pH dependence of the F3Y122* left arrow over right arrow

174 The pH dependence of the fast phase kinetic data shows that

175 The pH dependence of the first-order rate constant for decay

176 The rate's pH sensitivity reflects the pH dependence of the four-electron O2-H2O couple.

177 The pH dependence of the free energy of denaturation was des

178 Likewise, studies of the pH dependence of the histidine substitution indicate a s

179 We have found that the pH dependence of the hydrolysis reaction is log-linear,

180 The pH dependence of the IrOx open circuit potential (OCP) w

181 ng catalysis was examined by determining the pH dependence of the kcat/Km values with ethylnitronate

182 The pH dependence of the kinetic isotope effects with [1,1-(

183 termination of its metal-binding properties; pH dependence of the kinetic parameters k (cat), K(m) ,

184 The pH dependence of the kinetic parameters revealed that th

185 The pH dependence of the kinetic parameters reveals that one

186 The pH dependence of the kinetic parameters was determined t

187 The pH dependence of the kinetic parameters with the WT enzy

188 On the basis of kinetic properties, the pH dependence of the kinetic parameters, and structural

189 In this report, we have determined the pH dependence of the kinetic parameters, revealing that

190 uilibrium thermodynamics analyses as well as pH dependence of the kinetics revealed that the V region

191 The pH dependence of the kinetics was found to be fully cons

192 duced and quantified the previously observed pH dependence of the major conformation of Lys48-linked

193 A detailed study of the pH dependence of the midpoint potential of skeletal hors

194 Here we examine the pH dependence of the monomer-dimer-tetramer reaction, of

195 The pH dependence of the nonenzymatic pathway for conversion

196 The calculated strong pH dependence of the number of chlorides bound and the a

197 d with dried buffer that prevents any sample pH dependence of the observed color change.

198 The pH dependence of the observed rate constants for O-acyli

199 The pH dependence of the on- and off-rate constants for suga

200 esulting acid triad is central to the marked pH dependence of the optical-absorption relaxation kinet

201 sfully reproduce the experimentally observed pH dependence of the overall rate and H/D kinetic isotop

202 The experiments revealed a strong pH dependence of the oxidation of ACV and carboxy-ACV wi

203 ion being the key species accounting for the pH dependence of the oxidation.

204 penetration of the blood-brain barrier, the pH dependence of the oxime and amine ionizing groups of

205 The pH dependence of the PHM-catalyzed monooxygenation of da

206 The difference in pH dependence of the pKESB for cysteine and serine, the

207 dditionally, the structural models also show pH dependence of the protein structure itself, which pro

208 The zeta-potential/pH dependence of the Pu(IV) colloids is similar to that

209 The pH dependence of the rate constants, the correlation bet

210 -base catalysis, and this in accord with the pH dependence of the reaction rate for the natural and m

211 nism is also consistent with the bell-shaped pH dependence of the reaction rate.

212 The pH dependence of the reactions was investigated at cloud

213 A nonmonotonic super-Nernstian pH dependence of the redox peaks with increasing Fe cont

214 The pH dependence of the reduction potential E degrees for a

215 The pH dependence of the second-order rate constant k cat/ K

216 The pH dependence of the second-order rate constants of inhi

217 e stability of the autoinhibited complex and pH dependence of the secondary cleavage provide means fo

218 The pH dependence of the solid-state (67)Zn NMR lineshapes h

219 Additionally, the pH dependence of the solution and enzymatic reactions pr

220 Although H290 is important for pH dependence of the stability, it is not critical for d

221 sis of these isotope effects, along with the pH dependence of the steady-state kinetic parameters, li

222 electron transfer from Q(A) (*-) to Q(B) The pH dependence of the thermoluminescence associated with

223 Global analysis of the pH dependence of the trimer-dodecamer equilibrium reveal

224 The pH dependence of the uptake of [(3)H]-methyltetrahydrofo

225 The pH dependence of the water splitting reaction suggests a

226 We determined the pH dependence of these bimolecular rate constants and fo

227 Herein, we report the pH dependence of these tautomeric forms providing the fi

228 To unlock the pH dependence of these transient states with atomistic-l

229 fferent from wild-type, and furthermore, the pH dependence of this potential is not the same as for w

230 of conserved aspartate residues explains the pH dependence of this transition, and biochemical studie

231 The pH dependence of Trp-245 unquenching exhibits a pK(a) of

232 The pH dependence of V(max) for both glutamate variants yiel

233 and showed modest change or no change in the pH dependence of virus-membrane fusion.

234 The pH dependence of Vmax for wild-type DmTrxR has pKa value

235 ines to alanine residues did not abolish the pH dependence of ZnR/GPR39.

236 Analysis of the pH dependences of the gating and open channel parameters

237 The pH-dependence of backbone amide NMR resonances demonstra

238 dine protonation in the binding process, the pH-dependence of bile salt binding and internal dynamics

239 folding led to apparent cooperativity in the pH-dependence of folding, although each group titrated i

240 pH-dependence of GO emission is utilized to provide the

241 The pH-dependence of HWKK binding to polyU provides no evide

242 The pH-dependence of log 1/K(M) is a sigmoidal curve reachin

243 Substantial pH-dependence of maturation was observed upon substituti

244 ase pairing, was confirmed by studies of the pH-dependence of mismatching.

245 Through these structural effects and the pH-dependence of Mn(II)-metal competitive adsorption, sy

246 w the PA:receptor interaction influences the pH-dependence of pore formation; and how the pore functi

247 These results demonstrate that the acid pH-dependence of protein stability in these hyperthermop

248 e overcome this challenge by quantifying the pH-dependence of quinary interactions in living Escheric

249 The simple DLM describes well the pH-dependence of single adsorption edges.

250 optimized biochemical methods, we dissected pH-dependence of spontaneous and DM-DO-mediated class II

251 es in low salt, indicating that the observed pH-dependence of stability was primarily due to these th

252 take/release and thus resulting in different pH-dependence of the binding energy.

253 The pH-dependence of the catalytic parameters was measured u

254 etermining the substrate specificity and the pH-dependence of the catalytic rates, is poorly understo

255 The results indicate pH-dependence of the fumarate modulation with opposite b

256 The pH-dependence of the pre-steady-state phosphorylation of

257 of the C41 base triple to the metal ion- and pH-dependence of the reaction are examined.

258 t this site II, as well as at site I, confer pH dependence on HN's receptor avidity.

259 The pH dependence on the kinetic constants suggests that a s

260 Further studies of the pH dependence on the observed rectification support a su

261 es, suggesting that multiple residues confer pH dependence on the transport mechanism.

262 a function of extracellular solution pH, the pH-dependence on Mg isotope fractionation is thus due to

263 Model analysis suggests that the pH-dependence on turnover is primarily due to the pKa va

264 We use our probe to follow PD, and its pH dependence, on the surface of lipid vesicles composed

265 hylene shorter (Orn) resulted in significant pH dependence or lack of interaction, suggesting that na

266 This favorable pH dependence originates from several elements of struct

267 n the switches, we can rationally tune their pH dependence over more than 5 pH units.

268 Ca2+, the EPR-active Mn3+ exhibits a strong pH dependence (pH approximately 6.5-9) of its ligand-fie

269 However, their differences in pH dependence, phosphate and fluoride inhibition pattern

270 ty to design DNA-based switches with tunable pH dependence provides the opportunity to engineer pH na

271 ith the charge recombination process shows a pH dependence ranging from 2.3 eV at pH </= 7 and 1.2 eV

272 The near absence of an intrinsic pH dependence represents an advantage compared to hydrog

273 ts transport of riboflavin to have an acidic pH dependence, saturability (apparent Km = 1.4 +/- 0.5 m

274 Analysis of binding coefficients and pH dependence showed that (125)I-STa-binding in GC-C KO

275 Kinetic analyses and pH dependence studies of the wild type and variant enzym

276 pH dependence studies show that the reaction rate increa

277 In addition, pH dependence studies show that the relative abundance o

278 and Asn593, and kinetic isotope effects and pH-dependence studies of the wild-type enzyme.

279 A pH dependence study allowed the determination of the pK(

280 The EPR spectrum and its pH dependence suggest that the coordination environment

281 We found that H216 is responsible for this pH dependence, suggesting that protonation of H216 could

282 The pH dependence suggests that the active form is the neutr

283 This novel pH dependence suggests that the hydrolysis is determined

284 ics of imatinib binding to Abl kinase have a pH dependence that disappears when the DFG aspartate is

285 e concentration of the peptide, also shows a pH dependence that is described and discussed.

286 excited state by dissolved oxygen exhibits a pH dependence that parallels that of the excited state l

287 e voltages of the transistors demonstrated a pH-dependence that was linear over a wide range and coul

288 of the pHD peptides affect their potency and pH dependence; therefore, the sequence-structure-functio

289 charge movements within the PR and shift its pH dependence to acidic values.

290 lecular fragments narrows the source of this pH dependence to its N-methylthiazolium fragment.

291 Thermodynamic modeling of the pH dependence to receptor binding affinity reveals large

292 e investigated the mechanism underlying this pH dependence using a combined approach of site-directed

293 hibited larger OH reactivities at pH 10, the pH dependence was dramatically different between FA, the

294 The origin of the observed pH dependence was further narrowed to the protonation eq

295 Lead uptake was larger and pH dependence was greater for the (012) and (110) surfac

296 the temperature, concentration, solvent, and pH dependence, we extract a thermodynamic and kinetic ch

297 rt to explain the microscopic origin of this pH dependence, we studied Abeta11-25 fibrils using metho

298 sidues that are likely to play a role in the pH dependence were identified.

299 Na(+)/H(+) antiporters show a marked pH dependence, which is important for their physiologica

300 A/(k(cat)/KM)LA ratio was observed to have a pH dependence, which was fit with a titration curve (pKa