ライフサイエンスコーパス: pantothenate

1 nsation of pantoate and beta-alanine to form pantothenate.

2 location, and kinetic constants for ATP and pantothenate.

3 competitively inhibit the phosphorylation of pantothenate.

4 e medium) in the absence of thiamine excrete pantothenate.

5 ate to D-(-)-pantoate in the biosynthesis of pantothenate.

6 ad a conditional requirement for thiamine or pantothenate.

7 cofactors including biotin, riboflavin, and pantothenate.

8 rds from labeled essential nutrients such as pantothenate.

9 of CoA) was replaced with [(13)C(3)(15)N(1)]-pantothenate.

10 between PanK-III and its substrates ATP and pantothenate.

11 evels of leaf Beta-alanine (1.2- to 4-fold), pantothenate (3.2- to 4.1-fold) and total free amino aci

12 essential nutrient vitamin B5 (also known as pantothenate)(6,7).

13 ctive with an apparent K(m) of 28 microM for pantothenate-7-amino-4-methylcoumarin (pantothenate-AMC)

14 e hepatocytes (Hepa 1c1c7) in media in which pantothenate (a precursor of CoA) was replaced with [(13

15 uman malaria parasites rely on the uptake of pantothenate across the parasite plasma membrane for sur

16 Modeling these antimetabolites into the pantothenate active site predicts that they bind in the

17 dodiphosphate (AMPPNP).Mg(2+), AMPPNP.Mg(2+).pantothenate, ADP.Mg(2+).phosphopantothenate, and AMP ph

18 ies was optimized to >99% [(13)C(3)(15)N(1)]-pantothenate after three passages of the murine cells in

19 M for pantothenate-7-amino-4-methylcoumarin (pantothenate-AMC), which was converted to pantothenic ac

20 te kinase that showed evidence of binding to pantothenate, an essential nutrient Mtb synthesizes, but

21 ement of genes required for the synthesis of pantothenate, an essential vitamin deficient in the lous

22 rolling intracellular coenzyme A levels, and pantothenate analogs are growth-inhibiting antimetabolit

23 rasite Plasmodium falciparum have shown that pantothenate analogs interfere with pantothenate phospho

24 The pantothenate analogs N-pentylpantothenamide and N-heptyl

25 l or better than that of currently available pantothenate analogs.

26 utilize the N-alkylpantothenamide family of pantothenate analogues as alternative substrates, thus m

27 a ternary complex structure of PanK-III with pantothenate and ADP.

28 of beta-alanine, followed by the release of pantothenate and AMP.

29 the transplacental transfer of the vitamins pantothenate and biotin and the essential metabolite lip

30 is-Menten constant (Kt) for the transport of pantothenate and biotin in cDNA-transfected cells is 4.9

31 pressed in HeLa cells, induces Na+-dependent pantothenate and biotin transport activities.

32 mation phase: the pentose phosphate pathway, pantothenate and CoA biosynthesis and ascorbate and alda

33 le, gluconeogenesis, glutathione metabolism, pantothenate and CoA biosynthesis, and butanoate metabol

34 ion of PfPAT and its ability to deliver both pantothenate and fenpropimorph makes it an attractive ta

35 e, UDP-N-acetylglucosamine, glycine, serine, pantothenate and phosphocholine.

36 sites of the natural substrate and product, pantothenate and phosphopantothenate, respectively.

37 the adenosine moieties are buried while the pantothenate and pyrophosphate groups of the coenzyme ar

38 Glycosides of pantothenate and riboflavin appear to be minor products

39 ing phagosome is limiting for riboflavin and pantothenate and that Histoplasma virulence requires de

40 Riboflavin, pantothenate, and biotin auxotrophs of Histoplasma were

41 A Cryptococcus yeast produces the B-vitamin pantothenate, and co-culturing with Variovorax leads to

42 mine HCl, riboflavin, niacinamide, d-calcium pantothenate, and pyridoxine HCl; 50 microg each of d-bi

43 ated forms of pyridoxine, vitamin D, niacin, pantothenate, and riboflavin exist in nature, whereas gl

44 n synthesize thiamine if increased levels of pantothenate are present in the culture medium.

45 In the cell, pantothenate, ATP, and cysteine are required to synthesi

46 A Schu S4 DeltapanG strain is a pantothenate auxotroph and was genetically and chemicall

47 ty and immunogenicity of a double lysine and pantothenate auxotroph of Mycobacterium tuberculosis in

48 SCID mice infected with the pantothenate auxotroph survived significantly longer (25

49 has been known that E. coli yhhK strains are pantothenate auxotrophs, but the role of YhhK in pantoth

50 at Salmonella enterica yhhK strains are also pantothenate auxotrophs.

51 terium glutamicum panD(+) gene corrected the pantothenate auxotrophy of a S. enterica yhhK strain, su

52 which form a "lid" that folds over the open pantothenate binding groove.

53 Pantothenate binding induces a significant conformationa

54 A connection exists between pantothenate biosynthesis and thiamine biosynthesis in S

55 psis encoded homologues of the remaining two pantothenate biosynthesis enzymes from E. coli, l-aspart

56 findings highlight the importance of de novo pantothenate biosynthesis in limiting the intracellular

57 We explored the possibility of manipulating pantothenate biosynthesis in plants.

58 othenate auxotrophs, but the role of YhhK in pantothenate biosynthesis remained an enigma.

59 In addition, depletion of Pan6 (pantothenate biosynthesis) but not Bio2 function (biotin

60 nuating auxotrophic mutations in leucine and pantothenate biosynthesis.

61 , including photorespiration, methionine and pantothenate biosynthesis.

62 vity had previously been associated with the pantothenate biosynthetic gene panE.

63 The pantothenate biosynthetic pathway is well-established in

64 e reductase (EC 1.1.1.169), an enzyme in the pantothenate biosynthetic pathway, catalyzes the NADPH-d

65 is obligatorily dependent on Na+ and accepts pantothenate, biotin, and lipoate as substrates.

66 , induces inward currents in the presence of pantothenate, biotin, and lipoate in a Na+-, concentrati

67 which transports the water-soluble vitamins pantothenate, biotin, and lipoate, from a placental chor

68 The transporter is specific for pantothenate, biotin, and lipoate.

69 acterial pathogen that is able to synthesize pantothenate but is lacking the known ketopantoate reduc

70 children below the EAR for vitamins A and E, pantothenate, calcium, and fiber; 2) >10% above the ULs

71 Pantothenate can be biosynthesized or is taken up by cel

72 , metal acquisition, and the biosynthesis of pantothenate, coenzyme A, thiamine, menaquinone, siderop

73 a-ketopantoate to form D-(-)-pantoate in the pantothenate/coenzyme A biosynthetic pathway.

74 Pantothenate, commonly referred to as vitamin B(5), is a

75 fPAT mediated survival of yeast cells in low pantothenate concentrations and restored sensitivity of

76 ed FBS, due to lower contaminating unlabeled pantothenate content.

77 several proteins essential for the canonical pantothenate-cysteine-dependent CoA biosynthesis pathway

78 ts receiving 13.5 mmol choline plus 1.4 mmol pantothenate/d had a significant decline in urinary carn

79 In culture, media supplementation with pantothenate decreased PanB levels, which required CoaX.

80 nt assay using a novel fluorescently labeled pantothenate derivative.

81 ignificantly higher levels of phosphorylated pantothenate-derived metabolites and CoA in vivo and exc

82 ECF transporters for folate (ECF-FolT2) and pantothenate (ECF-PanT) into proteoliposomes, and assaye

83 We explore the therapeutic potential of pantothenate encapsulated in liposomes (Pan@TRF@Liposome

84 intermediates, TCA cycle intermediates, and pantothenate expand dramatically in both mitochondrial d

85 olutely dependent on the acquisition of host pantothenate for its development within human erythrocyt

86 ives of purF mutants that no longer required pantothenate for thiamine-independent growth were isolat

87 The rate of pantothenate formation from the adenylate and beta-alani

88 tase (EC 6.3.2.1) catalyzes the formation of pantothenate from ATP, D-pantoate, and beta-alanine in b

89 rium tuberculosis catalyzes the formation of pantothenate from ATP, D-pantoate, and beta-alanine.

90 ation of D-pantoate and beta-alanine to form pantothenate in bacteria, yeast and plants.

91 sotope exchange of [(14)C]-beta-alanine into pantothenate in the presence of AMP was observed, indica

92 ine metabolism was distinct from the role of pantothenate in thiamine synthesis.

93 depupylation of Pup~PanB, while addition of pantothenate inhibited this reaction.

94 hia coli incorporated pyridoxal, niacin, and pantothenate into pyridoxal 5'-phosphate, NAD, and coenz

95 Pantothenate is a key vitamin for the intracellular bios

96 Commercially, pantothenate is chemically synthesised and used in vitam

97 ement of panE mutants for either thiamine or pantothenate is manifest only when flux through the puri

98 Remarkably, we found that pantothenate is not incorporated into CoA as such but is

99 We show here that the effect of pantothenate is prevented by blocks in the oxidative pen

100 Pantothenate is the precursor of the essential cofactor

101 Pantothenate is the product of the ATP-dependent condens

102 K-III in complex with one of its substrates (pantothenate), its product (phosphopantothenate) as well

103 Pantothenate kinase (CoaA) catalyzes the first and regul

104 Pantothenate kinase (CoaA) is a key regulator of coenzym

105 CoA biosynthesis in bacteria and mammals is pantothenate kinase (CoaA), which governs the intracellu

106 In Bacillus anthracis, the novel type III pantothenate kinase (PanK(Ba); encoded by coaX) catalyze

107 al and animal coenzyme A (CoA) biosynthesis, pantothenate kinase (PANK) activity is critical in regul

108 In contrast, a novel type III pantothenate kinase (PanK) catalyzes the first committed

109 Pantothenate kinase (PanK) catalyzes the first step in t

110 Pantothenate kinase (PanK) catalyzes the first step of t

111 Pantothenate kinase (PanK) is a key regulatory enzyme in

112 Pantothenate kinase (PanK) is a rate-determining enzyme

113 Pantothenate kinase (PanK) is a regulatory enzyme that c

114 Pantothenate kinase (PanK) is known to catalyze the rate

115 Pantothenate kinase (PanK) is the key regulatory enzyme

116 Pantothenate kinase (PanK) is the key regulatory enzyme

117 y enzymatic phosphorylation with E. faecalis pantothenate kinase (PanK).

118 The mouse murine pantothenate kinase (Pank1) gene consists of seven intro

119 The human isoform 2 of pantothenate kinase (PanK2) is localized to the mitochon

120 miR107 is located in intron 5 of the pantothenate kinase 1 (PANK1) gene.

121 We identify pantothenate kinase 2 (PANK2) and PANK4 as substrates of

122 Mutations in the pantothenate kinase 2 (PANK2) gene have been identified

123 Pantothenate kinase 2 (PANK2) is an essential regulatory

124 disease have mutations in the gene encoding pantothenate kinase 2 (PANK2); these patients are said t

125 Mutations in the pantothenate kinase 2 gene cause a severe form of neurod

126 Predicted levels of pantothenate kinase 2 protein correlate with the severit

127 The PANK2 gene encodes the human pantothenate kinase 2 protein isoforms, and PANK2 mutati

128 In bacteria, regulation of pantothenate kinase activity is a major factor in contro

129 ramatically in a yeast mutant with defective pantothenate kinase activity.

130 gulator, and antimetabolite binding sites on pantothenate kinase and provide a framework for studies

131 e structural information on Escherichia coli pantothenate kinase by determining the structure of the

132 We used yeast and its pantothenate kinase Cab1 as models to characterize mode

133 Here, we show that the pantothenate kinase Cab1p, which catalyzes the first ste

134 Pantothenate kinase catalyzes a key regulatory step in c

135 Pantothenate kinase catalyzes the first step in the bios

136 atment for neurodegeneration associated with pantothenate kinase deficiency.

137 The two enzymes have homologous pantothenate kinase domains, but AtPANK2 also carries a

138 of two new classes of compounds that inhibit pantothenate kinase from M. tuberculosis are described,

139 s, including the finding of mutations in the pantothenate kinase gene and ferritin light chain gene,

140 ow that HSS is caused by a defect in a novel pantothenate kinase gene and propose a mechanism for oxi

141 Although two eukaryotic-type putative pantothenate kinase genes (PanK1 and PanK2) have been id

142 , especially given the existence of multiple pantothenate kinase genes in humans and multiple PanK2 t

143 spatial and chemical distribution of evolved pantothenate kinase immobilized onto two diverse, microp

144 perature-sensitive mutation of the bacterial pantothenate kinase in E. coli strain ts9.

145 Given the role of pantothenate kinase in production of Coenzyme A and in p

146 trate the key role of feedback regulation of pantothenate kinase in the control of intracellular CoA

147 ns indicate that this type of "bifunctional" pantothenate kinase is conserved in other higher eukaryo

148 Pantothenate kinase is the master regulator of CoA biosy

149 Pantothenate kinase isoform PanK3 is highly related to t

150 report here the characterization of a second pantothenate kinase of Arabidopsis, AtPANK2, as well as

151 The absence of feedback regulation at the pantothenate kinase step allows the accumulation of high

152 s to the highest resolution reported for any pantothenate kinase structure.

153 Pantothenate kinase, a key enzyme in the universal biosy

154 The activity of the second pantothenate kinase, AtPANK2, was confirmed by its abili

155 ate specificities associated with endogenous pantothenate kinase, the first enzyme in the CoA biosynt

156 icated in neurodegenerative diseases such as pantothenate kinase-associated neurodegeneration (PKAN)

157 Pantothenate Kinase-Associated Neurodegeneration (PKAN)

158 Pantothenate kinase-associated neurodegeneration (PKAN)

159 Pantothenate kinase-associated neurodegeneration (PKAN)

160 PANK2 mutations cause pantothenate kinase-associated neurodegeneration (PKAN),

161 Pantothenate kinase-associated neurodegeneration (PKAN,

162 den-Spatz syndrome, the disorder was renamed pantothenate kinase-associated neurodegeneration after d

163 e shown great potential for the treatment of pantothenate kinase-associated neurodegeneration and pro

164 Pantothenate kinase-associated neurodegeneration is a fo

165 PanK2(G521R), the most frequent mutation in pantothenate kinase-associated neurodegeneration, was de

166 In all patients with pantothenate kinase-associated neurodegeneration, whethe

167 isoforms, and PANK2 mutations are linked to pantothenate kinase-associated neurodegeneration.

168 e 2 (PANK2); these patients are said to have pantothenate kinase-associated neurodegeneration.

169 ndings in six cases of molecularly confirmed pantothenate kinase-associated neurodegeneration.

170 e identify prominent ubiquinated deposits in pantothenate kinase-associated neurodegeneration.

171 ackup" regulator of pathway flux relative to pantothenate kinase.

172 s the starting metabolite, phosphorylated by pantothenate kinase.

173 ural substrate PA for phosphorylation by the pantothenate kinase.

174 ions of the phosphoryl transfer mechanism of pantothenate kinase.

175 N-alkylpantothenamides are substrates for pantothenate kinase.

176 pathway and is mediated by four isoforms of pantothenate kinase.

177 ry properties exhibited by the family of the pantothenate kinases allowed the rate of CoA biosynthesi

178 , both active sites of the dimeric mammalian pantothenate kinases coordinately switch between the on

179 coside phosphotransferase [APH(3')-IIIa] and pantothenate kinases from Escherichia coli (EcPanK) and

180 contain a domain with high similarity to the pantothenate kinases of A. nidulans and mouse.

181 berculosis enzyme but similar to that in the pantothenate kinases of other organisms.

182 quence that is more similar to the mammalian pantothenate kinases than the prototypical bacterial Coa

183 in human brain, distinguishing it from other pantothenate kinases that do not possess mitochondrial-t

184 In contrast to all known pantothenate kinases, SaCoaA activity is not feedback-re

185 CoaX regulates PanB abundance in response to pantothenate levels by modulating its vulnerability to p

186 ontrast plants expressing KPHMT had elevated pantothenate levels in leaves, flowers siliques and seed

187 No significant change of pantothenate levels in PS transgenic lines was observed.

188 delian randomization analysis that elevating pantothenate levels significantly contributes to the pro

189 lants are capable of de novo biosynthesis of pantothenate, making this pathway a potential target for

190 both purine and thiamine; however, exogenous pantothenate may be substituted for the thiamine require

191 Flavobacteriia and Pantothenate may potentially serve as novel biomarkers t

192 the effects of supplementary choline and/or pantothenate on the carnitine and lipid status of free-l

193 nstream compounds in the coenzyme A pathway, pantothenate or beta-alanine.

194 hip between the cDNA-specific uptake rate of pantothenate or biotin and Na+ concentration is sigmoida

195 Low-RFI steers had greater abundances of pantothenate (P = 0.02) based on fold change (high/low R

196 lysis of DEGs in DeltaMAP3773c revealed that pantothenate (Pan) biosynthesis, polysaccharide biosynth

197 r proteins responsible for the conversion of pantothenate (Pan) to CoASH in Escherichia coli are cons

198 In summary, we characterized the pantothenate pathway in Francisella novicida and F. tula

199 own that pantothenate analogs interfere with pantothenate phosphorylation and block asexual blood sta

200 n normal cells providing clear evidence that pantothenate phosphorylation was a rate-controlling step

201 teracting with the hydrophobic dome over the pantothenate pocket, which is also accessed by the beta-

202 beta expression eliminated the intracellular pantothenate pool and triggered a 13-fold increase in in

203 Fusobacteriia and/or greater proportions of pantothenate-producing bacteria, such as Flavobacteriia,

204 ns, was necessary and sufficient to increase pantothenate production and allow PurF-independent thiam

205 s took 0.20 mmol and 0.02 mmol/kg choline or pantothenate, respectively.

206 rences in the binding modes for both ATP and pantothenate substrates, and suggests that these differe

207 Choline, but not pantothenate, supplementation significantly decreased ur

208 dent multivitamin transporter for biotin and pantothenate), SVCT (for vitamin C), and CaT1 (for Ca up

209 In a DeltacoaX mutant, pantothenate synthesis enzymes including PanB, a substra

210 The lack of a de novo pantothenate synthesis pathway allowed for efficient and

211 Pantothenate synthetase (EC 6.3.2.1) catalyzes the forma

212 Pantothenate synthetase (EC 6.3.2.1), encoded by the pan

213 T), L: -aspartate-alpha-decarboxylase (ADC), pantothenate synthetase (PS) and ketopantoate reductase

214 Pantothenate synthetase (PS) from Mycobacterium tubercul

215 Pantothenate synthetase (PS) is one of the potential new

216 antoate hydroxymethyltransferase (KPHMT) and pantothenate synthetase (PtS) catalyse the first and las

217 Protein kinase B and pantothenate synthetase are examined in detail.

218 e reactions have therefore demonstrated that pantothenate synthetase catalyzes the formation of a pan

219 9, Q72, and K160 residues in M. tuberculosis pantothenate synthetase caused a greater than 1000-fold

220 hree-dimensional structural determination of pantothenate synthetase from Mycobacterium tuberculosis

221 Pantothenate synthetase from Mycobacterium tuberculosis

222 Pantothenate synthetase from Mycobacterium tuberculosis

223 [betagamma-(18)O(6)]-ATP was incubated with pantothenate synthetase in the presence of d-pantoate, a

224 ion of pantoyl adenylate intermediate in the pantothenate synthetase reaction.

225 a kinetically competent intermediate in the pantothenate synthetase reaction.

226 tion studies showed the kinetic mechanism of pantothenate synthetase to be Bi Uni Uni Bi Ping Pong, w

227 ase of pyrophosphate from the active site of pantothenate synthetase.

228 strain MG1655DeltapanC lacking a functional pantothenate synthetase.

229 alidation against Mycobacterium tuberculosis pantothenate synthetase.

230 determining the structure of the enzyme.ADP.pantothenate ternary complex.

231 In contrast to the uptake mechanisms for pantothenate, the cellular uptake mechanisms for PanSH a

232 ) coupled with hydrogen bonding of the C1 of pantothenate to Asp-127 suggests different interpretatio

233 ha-decarboxylase (PanD) enzyme that converts pantothenate to beta-alanine.

234 Conversion of pantothenate to phosphopantothenate in humans is the fir

235 ribose 5-P may play a role in the ability of pantothenate to substitute for thiamine.

236 vidence for an essential role of a candidate pantothenate transport in malaria transmission to Anophe

237 Recently, a P. falciparum candidate pantothenate transporter (PAT) was characterized by func

238 e molecular identity of the parasite-encoded pantothenate transporter remains unknown.

239 onal characterization of the first protozoan pantothenate transporter, PfPAT, from P. falciparum.

240 nts the growth defect of the yeast fen2Delta pantothenate transporter-deficient mutant and mediates t

241 restored sensitivity of yeast cells lacking pantothenate uptake to fenpropimorph.

242 e canonical five-step pathway, starting with pantothenate uptake.

243 armacological analyses, we show that altered pantothenate utilization dramatically alters the suscept

244 uggesting a preference for beta-alanine over pantothenate utilization in CoA synthesis.

245 nce for the importance of the early steps of pantothenate utilization in the regulation of CoA biosyn

246 reduction of ketopantoate to pantoate on the pantothenate (vitamin B(5)) biosynthetic pathway.

247 Pantothenate (vitamin B(5)) is the precursor of the 4'-p

248 s in cell culture to include [(13)C(3)(15)N]-pantothenate (vitamin B(5)), a CoA precursor, instead of

249 1.169), is essential for the biosynthesis of pantothenate (vitamin B(5)).

250 llaboration in branched-chain amino acid and pantothenate (vitamin B5) biosynthesis.

251 Pantothenate (vitamin B5) is the precursor for the biosy

252 In nearly all non-photosynthetic cells, pantothenate (vitamin B5) transport and utilization are

253 Pantothenate (vitamin B5) was significantly increased in

254 Pantothenate (Vitamin B5) was significantly increased in

255 fold), nicotinamide/vitamin B(3) (1.5-fold), pantothenate/vitamin B(5) (1.3-fold), and pyridoxine/vit

256 Pantothenate was the most abundant pathway component (42

257 tions for the formation of pyrophosphate and pantothenate were determined using rapid quench techniqu

258 mational changes upon binding of folate than pantothenate, which could explain the kinetic difference

259 thiamine auxotrophs are predicted to produce pantothenate, which we show is required for growth of Va

260 thenate was prepared by hydrolysis of methyl pantothenate with Na(18)OH, followed by enzymatic phosph

261 ts that they bind in the same orientation as pantothenate with their alkyl chains interacting with th