ライフサイエンスコーパス: paxillin

1 rigin of intracellular signaling via FAK and paxillin.

2 tion, which can be reversed by knocking down paxillin.

3 ylation of MYPT1 T853, MYPT1 T696, MBS85 and paxillin.

4 d the focal adhesion molecules FAK, Pyk2 and paxillin.

5 urs locally by two-channel imaging of RF and paxillin.

6 glycogen synthase kinase 3beta (GSK3beta) or paxillin.

7 ked the LD2 motif of the FAK binding partner paxillin.

8 ent to recruit the signaling adapter protein paxillin.

9 wnstream effectors focal adhesion kinase and paxillin.

10 ntegral focal adhesion proteins vinculin and paxillin.

11 expansion, involving positive regulation of Paxillin.

12 ctivation of focal adhesion kinase (FAK) and paxillin.

13 ed activation of the focal adhesion protein, paxillin.

14 egulatory proteins HSP20, VASP, cofilin, and paxillin.

15 nduced Akt- and MAPK-dependent activation of paxillin.

16 omplex formation through an interaction with paxillin.

17 binant VHR directly dephosphorylated FAK and paxillin.

18 and phosphorylation of RLC, MYPT1, MBS85 and paxillin.

19 evertheless, the LD2-LD4 region of paxillin (paxillin(133-290)) binds to Pyk2-FAT as a 1:1 complex.

20 Overexpression of paxillin, a cell adhesion protein downstream of NAD+ in

21 tly showed that the ability of Cat-1 to bind paxillin, a major constituent of focal complexes, is als

22 angiogenic sprouting through PAK2-dependent paxillin activation and remodeling of focal adhesions, w

23 mic NPLY(747) motif, to induce spreading and paxillin adapter recruitment to substrate- and talin-bou

24 Nuclear paxillin also complexed with ERK and ELK1, mediating c-F

25 cal adhesion targeting (FAT) domain binds to paxillin, an adhesion molecule.

26 sformation is coupled to its ability to bind paxillin and abrogate its actions as a negative regulato

27 CS induced phosphorylation of paxillin and activated p42/44-MAP kinase, Rho GTPase, an

28 decreased ILK levels and reduced binding to paxillin and alpha-parvin.

29 GIT1 led to enhanced protein degradation of paxillin and alpha5beta1 integrin via proteasome and lys

30 Pyk2 at Tyr(402) and of the Pyk2 substrates paxillin and ASAP1.

31 hesion and cell migration by phosphorylating paxillin and beta-catenin.

32 Using mutants of paxillin and chimeric proteins between HIC-5 and paxilli

33 PT1 T696, myosin binding subunit 85 (MBS85), paxillin and CPI-17 (PKC-potentiated protein phosphatase

34 bitor (Lck-I), blocks the phosphorylation of Paxillin and Crk-II, the formation of pseudopodia and th

35 se inhibitor reveals a pathway that involves paxillin and CrkII, which ultimately elevates Rac-GTP le

36 xhibiting greater levels of chaos than KRAS, paxillin and EGFR.

37 aling partner of paxillin, and suggests that paxillin and FAK function cell-autonomously to control m

38 identify an important collaboration between paxillin and FAK signaling in the modulation of microtub

39 The ACh-induced recruitment of paxillin and FAK to the cell membrane was dependent on R

40 co-transfected with either Myc-Hic-5 or Myc-paxillin and FLAG-tagged Smads 1, 5 or 8.

41 infected cells the vertical displacement of paxillin and focal adhesion kinase from the signaling la

42 including normal phosphorylation of FAK and paxillin and formation of fibrillar adhesions.

43 Paxillin and Hic-5 are homologous focal adhesion adaptor

44 orylation of focal adhesion proteins such as paxillin and Hic-5 are important for actin cytoskeleton

45 -parvin to focal adhesions requires both the paxillin and integrin-linked kinase binding sites and th

46 ltering the regulation of talin/integrin/FAK/paxillin and integrin/NFkappaB signaling pathways.

47 ty of actin cytoskeletal proteins, including paxillin and LPP.

48 tion of the cytoskeletal scaffolding protein paxillin and other host regulatory proteins, as well as

49 angements, including F-actin redistribution, paxillin and PAK4 phosphorylation, and beta1-integrin ac

50 erin-11 co-localizes with beta1-integrin and paxillin and physically interacts with the fibronectin-b

51 ntly regulates Src/focal adhesion kinase and paxillin and prevents anoikis.

52 am signaling, such as the phosphorylation of paxillin and signal transducer and activator of transcri

53 h CD44 and CD49d get access to src, FAK, and paxillin and via lck to the MAPK pathway, with the latte

54 ments demonstrated that adhesions containing paxillin and vinculin can form without talin following i

55 ocal adhesion proteins including ILK, PINCH, paxillin, and cdc42, as well as regulating the epithelia

56 e 1) that glucose-induced activation of FAK, paxillin, and ERK1/2 is mediated by beta1 integrin intra

57 identify three LIM protein families (zyxin, paxillin, and FHL) whose members preferentially localize

58 ma, protein kinase C, focal adhesion kinase, paxillin, and mitogen-activated protein kinases 1, 12, a

59 d increased tyrosine phosphorylation of Cas, paxillin, and Pyk2, which were previously also implicate

60 emingly through its capacity to control Cas, paxillin, and Pyk2.

61 dhesion kinase (FAK), a signaling partner of paxillin, and suggests that paxillin and FAK function ce

62 ppreciated relationship between Src kinases, paxillin, and survival of breast cancer patients.

63 e expression of focal adhesion kinase (FAK), paxillin, and the formation of FAK/Src/Paxillin complex,

64 e, we show that focal adhesion proteins FAK, paxillin, and vinculin but not the cytoskeletal protein

65 Mice with a neural-specific deletion of paxillin are also postnatal viable, but show evidence of

66 Talin, vinculin, and paxillin are core components of the dynamic link between

67 ctions between Cat-1 and its binding partner paxillin are necessary to ensure sufficient Akt activati

68 and the GIT1 ARF-GAP protein for binding to paxillin are required but not sufficient for transformat

69 icates that at least six distinct domains of paxillin are required for E6 transformation.

70 odeling of focal adhesions and that PAK2 and paxillin are required for EC polarization, migration, an

71 Our results highlight paxillin as a core molecule in substrate modulus-control

72 Here, we identified paxillin as a new Plk1-interacting protein in human airw

73 To summarize, we identified paxillin as a new regulator protein of PASMC growth.

74 lial cell line U2OS was transfected with GFP-paxillin as an FA marker and subjected to sustained unia

75 Here, we identify PAK2 and paxillin as critical targets of Ang-1 responsible for EC

76 LD motif (LD1) of the FA-associated protein paxillin as the binding partner of the p130Cas CCHD (in

77 tion at the nanoscale and recruit a suite of paxillin-associated proteins implicated in invasion and

78 that the assembly of FAK-FAs occurs ahead of paxillin at cell front.

79 dynamics of focal adhesion kinase (FAK) and paxillin at FAs in the protrusion of living endothelial

80 difference between the assemblies of FAK and paxillin at nascent FAs, FAs containing both FAK and pax

81 n, we provide evidence of an accumulation of paxillin at the centrosome that is dependent on focal ad

82 d Rho activity, indicating that the MLK3-JNK-paxillin axis limits Rho activity to promote focal adhes

83 by stable MTs and highlight how an MT/HDAC6/paxillin axis participates in the regulation of AChR ins

84 d FAK (V744G), we find that calpain inhibits paxillin-based adhesion assembly through cleavage of tal

85 lamellipodia membrane and a strengthening of paxillin-based focal adhesion within the same lamellipod

86 sing endogenous wild-type ILK, implying that paxillin binding to ILK is required for its localization

87 self-association, Tyr(925) phosphorylation, paxillin binding, and FA targeting and turnover.

88 Rac1 activity achieved by preventing alpha4/paxillin binding, confined migration requires myosin II-

89 ctions, including recruitment to FAs through paxillin binding.

90 s increased when Rac1 is inhibited by alpha4/paxillin binding.

91 On soft substrates, most paxillin binds to endocytic factors and facilitates vesi

92 Phosphorylation of paxillin by Src family kinases, which regulates adhesion

93 Paxillin, by modulating microtubule acetylation through

94 We also found that paxillin can bind to both talin and vinculin when either

95 egulated upon adhesion, including PYK2/FAK2, Paxillin, CASL and p130CAS consistent with focal adhesio

96 ivation, paxillin Ser273 phosphorylation and paxillin complex assembly.

97 ever, our data suggest that the Pyk2-FAT and paxillin complex is dynamic and it appears to be a mixtu

98 Assembly of the Pak-GIT1-betaPIX-paxillin complex was necessary for cdc42 and neuronal Wi

99 FAK), paxillin, and the formation of FAK/Src/Paxillin complex, which are correlated with cell adhesio

100 Here, using a newly developed paxillin conditional knockout mouse model with targeted

101 hanced the assembly and disassembly rates of paxillin-containing adhesions in an SHCA-dependent manne

102 ation, contractile actin bundles and dynamic paxillin-containing adhesions in the leading process and

103 These vimentin filaments link to paxillin-containing focal adhesions at the lamellipodial

104 the cellular focal adhesion adapter protein paxillin contributes to cell transformation and extends

105 Our observations lead to a model whereby paxillin contributes to talin and vinculin recruitment i

106 We found nuclear paxillin could then associate with androgen-stimulated a

107 lysome-bound RNA reveals transcripts of Lck, Paxillin, Crk-II, and Rac1 that undergo local translatio

108 Paxillin-deficient neurons have shorter leading processe

109 Multiphoton imaging revealed that paxillin-deficient neurons migrate approximately 30% slo

110 d non-phosphorylatable paxillin Y118/31F and paxillin DeltaLD4 deletion mutants in SM tissues.

111 th Cdc42 in response to Ang-1 in a PAK2- and paxillin-dependent manner.

112 esion kinase (FAK), FAK(PY397), F actin, and paxillin-dependent protrusion formation localizing to th

113 bitor tubacin highlight the central role for paxillin-dependent regulation of HDAC6 activity and asso

114 Paxillin depletion also resulted in reduced levels of ap

115 Because paxillin depletion did not affect ILK localization to FA

116 h using the example of HeLa cells expressing paxillin-EGFP to visualize focal adhesions.

117 d phosphorylation of pathways involving MET, paxillin, EPHA2, and VEGFR.

118 We show that knocking down paxillin expression in HeLa cells promotes their ability

119 ne intrapulmonary arteries revealed elevated paxillin expression in hypoxia-induced PH.

120 Silencing of paxillin expression led to decreased PASMC adhesion, pro

121 ic pulmonary arterial hypertension patients, paxillin expression was increased on mRNA and protein le

122 tein fibronectin was critically dependent on paxillin expression.

123 igrating ECs, which is dependent on PAK2 and paxillin expression.

124 We found that vinculin and paxillin FA area did not correlate with traction force m

125 The significantly higher FAK FI than paxillin FI at cell front indicates that the assembly of

126 illin in migrating cells, the normalized FAK/Paxillin fluorescence intensity (FI) ratio is > 1 ( appr

127 to 0.58), respectively, whereas the average paxillin focal-adhesion-cluster (FAC, formed at poles) l

128 ttom of the cell through a pathway including paxillin, focal adhesion kinase (FAK) and vinculin.

129 cell adhesion, and motility via cleavage of paxillin, focal adhesion kinase, and talin).

130 e insight into the underlying selectivity of paxillin for Pyk2 and FAK that may influence the differi

131 on of KSHV-TK also induces a loss of FAK and paxillin from focal adhesions, resulting in activation o

132 esions, increase RhoA activity and sequester paxillin from the endocytic machinery, thereby delaying

133 This study proposes a novel concept of paxillin-GEF-H1-p42/44-MAPK module as a regulator of pat

134 sphorylation mutant decreased the CS-induced paxillin/GEF-H1 association (16.3 +/- 4.1%), GEF-H1 acti

135 ibition of p42/44-MAPK suppressed CS-induced paxillin/GEF-H1 interactions (15.9 +/- 7.9%), GEF-H1 act

136 activated p42/44-MAP kinase, Rho GTPase, and paxillin/GEF-H1/p42/44-MAPK association.

137 eased activation of the cytoskeletal protein paxillin, growth factor-induced ischemic retinopathy in

138 Paxillin has five leucine-aspartate (LD) motifs (LD1-LD5

139 ed to bind to 14-3-3-zeta and associate with paxillin in a ternary complex that is regulated by serin

140 sABs are capable of pulling down endogenous paxillin in complex with FAK and can visualize paxillin

141 ly, these data reveal a fundamental role for paxillin in coordinating MT acetylation-dependent cell p

142 xillin in complex with FAK and can visualize paxillin in focal adhesions in cells.

143 our data implicates beta1 integrin, FAK, and paxillin in mediating the observed pro-adhesive effects

144 By tracking and quantifying FAK and paxillin in migrating cells, the normalized FAK/Paxillin

145 identified a cell-autonomous requirement for paxillin in migrating neurons.

146 nd its interactions with the Arf GTPases and paxillin in oncogenic transformation.

147 Specific deletion of paxillin in postmitotic neurons using Nex-Cre-mediated r

148 opy (SMLM) to investigate integrin beta3 and paxillin in rat embryonic fibroblasts growing on two dif

149 role for the focal adhesion scaffold protein paxillin in regulating the posttranslational modificatio

150 e it forms a signaling complex with PAK2 and paxillin in response to Ang-1.

151 on of ErbB2, ERK, focal adhesion kinase, and paxillin in response to NRG1, but fail to increase in si

152 s predictions with measured distributions of paxillin in spreading fibroblasts.

153 nd acini cultures, we identify new roles for paxillin in the establishment of apical-basal epithelial

154 AK assembles at the nascent FAs earlier than paxillin in the protrusions at cell front.

155 in soft agar, whereas ectopically expressing paxillin in these cells inhibits this transformed growth

156 hesion remodeling and the phosphorylation of paxillin independently of upstream regulation by focal a

157 Actopaxin (alpha-parvin) is a paxillin, integrin-linked kinase, and F-actin binding fo

158 about the underlying mechanism by which Cat-paxillin interactions mediate this effect.

159 isruption of focal adhesion kinase (FAK) and paxillin interactions using the small molecule JP-153 in

160 ng "platforms" and are capable of inhibiting paxillin interactions, thereby useful as potential thera

161 Moreover, the endogenous HDAC6 inhibitor paxillin interacts with HDAC6 in skeletal muscle cells,

162 While the focal adhesion adapter protein paxillin is a well-characterized regulator of mesenchyma

163 cules to modulate the focal adhesion protein paxillin is an effective strategy for treating pathologi

164 ntegrin-linked kinase binding sites and that paxillin is important for early targeting of beta-parvin

165 e that MAPK-dependent targeting of GEF-H1 to paxillin is involved in the regulation of CS-induced Rho

166 lls from forming colonies in soft agar, when paxillin is knocked down together with Cat-1, the cells

167 Because paxillin is one of the key scaffold molecules in FAs, we

168 e the similarity between HIC-5 and paxillin, paxillin is required for E6 to transform mouse embryo fi

169 Paxillin is shown to be required for the integrity and a

170 asp-2 with its binding partners vinculin and paxillin is significantly reduced in the presence of las

171 Paxillin is therefore a potential biomarker for prostate

172 that a critical difference between HIC-5 and paxillin is within the LIM domains of paxillin that do n

173 that an interaction with another CA protein, paxillin, is essential for correct localization of FAK i

174 -5, a close relative of the scaffold protein paxillin, is essential for the formation and organizatio

175 thelial ICAM-1/JNK led to phosphorylation of paxillin, its association with VE-cadherin, and internal

176 in EC permeability, which was attenuated by paxillin knockdown.

177 In the absence of FAK or paxillin, KSHV-TK has no effect on focal adhesion integr

178 The sABs are tools for investigation of paxillin LD binding "platforms" and are capable of inhib

179 ture of the p130Cas CCHD in complex with the paxillin LD1 motif by X-ray crystallography.

180 re of beta-parvin CH2 domain in complex with paxillin LD1 motif to 2.9 A resolution and find that the

181 previously observed between alpha-parvin and paxillin LD1.

182 a indicate that a prokaryotic version of the paxillin LD2 domain targets the FAK signaling pathway, w

183 Here, we show that the second LD motif of paxillin, LD2, interacts with Pyk2-FAT, similar to the k

184 ealed a similar level of interaction between paxillin-LD2 and TarP-LD.

185 o, COL6 promoted steady-state phosphorylated paxillin levels and reduced cell density (16% to 28%; P

186 domain region from the fission yeast protein paxillin like 1 (Pxl1) also localizes to SFSS in mammali

187 tion, and genetic approaches, we reveal that paxillin-linked endocytosis and adhesion are components

188 Focal adhesion protein paxillin links integrin and growth factor signaling to a

189 tionship between traction force and vinculin-paxillin localization to single FAs in the context of su

190 hat the enhanced interaction of vinculin and paxillin may functionally destabilize focal adhesion com

191 Herein, we identify new roles for paxillin-mediated HDAC6 inhibition in regulating key asp

192 Paxillin mediates stretch-induced Rho signaling and endo

193 s can form in the absence of paxillin or any paxillin member.

194 ced expression of adhesion complex molecules paxillin, metavincullin, and talin.

195 uced paxillin phosphorylation on Ser273; the paxillin mutant, paxillin S273A, inhibited paxillin Ser2

196 l kinase axis resulting in the activation of paxillin, NF-kappaB, and matrix metalloproteinase-2 (MMP

197 dues (ILK-VT/GG) could neither interact with paxillin nor localize to FA in cells expressing endogeno

198 osphorylation of the focal adhesion scaffold paxillin on Ser 178 and Tyr 118, which was blocked by si

199 eracting target 1 (GIT1), phosphorylation of paxillin on Ser273, and formation and distribution of ad

200 hosphorylates the adhesion junction protein, paxillin, on Ser273, which promotes the formation of a s

201 atches, podosomes can form in the absence of paxillin or any paxillin member.

202 Our results indicate that the paxillin/p110gamma-PI3K/Cdc42/Rac1 axis is defective in

203 and endothelial permeability via assembly of paxillin-p42/44MAPK-GEF-H1 complex.

204 nsion to test the hypothesis that FA protein paxillin participates in CS-induced Rho activation by re

205 similarly as in the structures with natural paxillin partners.

206 stablish a requirement for the Lhx9-integrin-paxillin pathway in proepicardial organ positioning and

207 r loss of Lhx9 or disruption of the integrin-paxillin pathway results in mis-positioning of the proep

208 Nevertheless, the LD2-LD4 region of paxillin (paxillin(133-290)) binds to Pyk2-FAT as a 1:1

209 Despite the similarity between HIC-5 and paxillin, paxillin is required for E6 to transform mouse

210 L6 is associated with reductions in integrin-paxillin-phosphatidylinositol 3-kinase signaling in vivo

211 which controls PTP-PEST activity and thereby paxillin phosphorylation and downstream signaling.

212 ACh induced paxillin phosphorylation and its association with the cd

213 eupaxin into podosomes and thereby regulates paxillin phosphorylation and podosome turnover.

214 We found that Ang-1 increases PAK2-dependent paxillin phosphorylation and remodeling of focal adhesio

215 the airway smooth muscle layer by affecting paxillin phosphorylation at this position.

216 gens enhanced airway smooth muscle layer and paxillin phosphorylation at this residue in mice, which

217 rmore, initiation of downstream signaling by paxillin phosphorylation in residue Y118 was evident, ev

218 cascades, including MEK1/ERK activation and paxillin phosphorylation on S126, which in turn triggers

219 tion and cell division in part by modulating paxillin phosphorylation on Ser-272.

220 Pak activation induced paxillin phosphorylation on Ser273; the paxillin mutant,

221 on of integrins and FAK but stronger FAK and paxillin phosphorylation upon attachment to fibronectin.

222 Akt, the formation of a spatial gradient in paxillin phosphorylation, and the activation of RhoA.

223 yrosine phosphatase PTP-PEST, which controls paxillin phosphorylation, requires leupaxin.

224 ivity and ROCK-RhoA levels but low levels of paxillin phosphorylation.

225 reduced focal adhesion turnover and enhanced paxillin phosphorylation.

226 etween the p130Cas CCHD and the LD1 motif of paxillin plays an important role in p130Cas FA targeting

227 ncrease in the size of focal-adhesion-kinase/paxillin-positive peripheral adhesions and reduced migra

228 (ITGB1), as well as phosphorylated forms of paxillin (pPXN) and focal adhesion kinase (pFAK).

229 dent phosphorylation blocks Cdc15 binding to paxillin Pxl1 and C2 domain protein Fic1 and enhances Cd

230 Amphiphysin-Rvs167 (F-BAR) protein Cdc15 and paxillin Pxl1.

231 srupts Cdc15's ability to bind membranes and paxillin, Pxl1, thereby inhibiting Cdc15's function in c

232 te cell attachment and cell migration called paxillin (PXN) and HIC-5 (also known as HIC5, ARA55, HIC

233 show that Kindlin-3 directly interacts with paxillin (PXN) and leupaxin (LPXN) via G43/L47 within it

234 sphorylation of the focal adhesion component paxillin (PXN) in a VEGF/VEGFR2-independent but NRP1-dep

235 in and the focal adhesion-associated protein paxillin (Pxn) in downstream signaling and functional ac

236 Paxillin (PXN), a key component of the focal adhesion co

237 ase (FAK) and the downstream adaptor protein paxillin (PXN), resulting in enhanced cell migration and

238 ion of integrin alpha6, integrin beta4, Fak, paxillin, Rac1/2/3, and ROCK1 in vitro.

239 Paxillin recruitment and residence time at FAs, however,

240 sharing the same binding mode with leupaxin, paxillin recruitment into podosomes is kindlin-3 indepen

241 assembled at FA first, which was followed by paxillin recruitment to the FA.

242 dependent regulation of the MT cytoskeleton, paxillin regulates both Golgi organelle integrity and po

243 estingly, we find that the scaffold molecule paxillin regulates both processes, making it a critical

244 In contrast, paxillin residence time at FAs was independent of local

245 orylation of focal adhesion kinase (FAK) and paxillin resulting in FA disassembly.

246 ctomyosin bundles move more quickly than the paxillin-rich adhesion plaques, which in turn move more

247 of MLK3, inhibition of JNK, or expression of paxillin S178A all led to enhanced Rho activity, indicat

248 sphorylation on Ser273; the paxillin mutant, paxillin S273A, inhibited paxillin Ser273 phosphorylatio

249 lamin B1, nonmuscle myosin heavy chain IIB, paxillin, Sec61 beta, tight junction protein ZO1, and To

250 Plk1 knockdown inhibited paxillin Ser-272 phosphorylation, centrosome maturation,

251 We unexpectedly found that phosphorylated paxillin (Ser-272) was localized in centrosomes of human

252 e paxillin mutant, paxillin S273A, inhibited paxillin Ser273 phosphorylation and inhibited actin poly

253 f Pak to adhesion junctions, Pak activation, paxillin Ser273 phosphorylation and paxillin complex ass

254 ociated cells showed that Pak activation and paxillin Ser273 phosphorylation triggered the formation

255 s to reduced adhesion turnover downstream of paxillin serine phosphorylation, which is rescued by add

256 issue post-SBI, Robo4 remained unchanged and Paxillin showed a decreasing trend.

257 Most of paxillin signaling activity is regulated via leucine-ric

258 we found that Lhx9 acts upstream of integrin-paxillin signaling and consistently demonstrate that eit

259 hat VEGF-dependent activation of the Src/FAK/paxillin signalsome is required for human retinal endoth

260 ac1 activity which was reversed by Robo4 and Paxillin siRNA.

261 y to alpha-parvin, beta-parvin does not bind paxillin, suggesting distinct interactions and cellular

262 aining region of 18 proteins from the Zyxin, Paxillin, Tes, and Enigma proteins accumulate to SFSS.

263 e a mixture of two distinct conformations of paxillin that almost equally compete for Pyk2-FAT bindin

264 , relaying signals from cell-ECM adhesion to paxillin that control cell migration and proliferation.

265 -5 and paxillin is within the LIM domains of paxillin that do not directly interact with E6.

266 an adhesion junction signalling complex with paxillin that included G-protein-coupled receptor kinase

267 Lamina ancestor (LAMA), the scaffold protein Paxillin, the endocytotic regulator Shibire (Dynamin), a

268 h the interaction of lasp-2 with vinculin or paxillin, this effect is greatly diminished in the prese

269 We observe recruitment of phosphorylated paxillin to activated EGFR at these patterned features,

270 n as well as the recruitment of vinculin and paxillin to adhesome signalling complexes in response to

271 the first paradigm validating modulation of paxillin to inhibit angiogenesis.

272 rotrusion by directly binding and recruiting paxillin to nascent adhesions.

273 merization of fibronectin and recruitment of paxillin to sites of lateral integrin alpha5beta1 cluste

274 Instead, we found paxillin together with talin and vinculin in initial adh

275 contractility regulated whether vinculin and paxillin turnover dynamics are correlated to each other

276 Galpha13, gastrin-induced FAK Tyr(P)-397 and paxillin Tyr(P)-31 phosphorylation were reduced.

277 n formation, with reduced phosphorylation of paxillin (Tyr(188)).

278 se-induced phosphorylation of FAK (Tyr-397), paxillin (Tyr-118), and ERK1/2 (Thr-202/Tyr-204).

279 markedly inhibits cell-ECM adhesion-induced paxillin tyrosine phosphorylation, cell migration, and p

280 role during initial cell spreading: it binds paxillin via the pleckstrin homology and F0 domains to a

281 lassical invadosomes, as they do not contain paxillin, vinculin, and beta1/beta3 integrins.

282 Paxillin was also required for pericentrosomal Golgi loc

283 Finally, paxillin was also shown to be required for optimal anter

284 rthermore, hypoxia-dependent upregulation of paxillin was HIF-1alpha dependent.

285 mportantly ICAM-1-induced phosphorylation of paxillin was required for lymphocyte TEM and converged f

286 Myc-Hic-5 but not Myc-paxillin was specifically immunoprecipitated with anti-F

287 llin and chimeric proteins between HIC-5 and paxillin, we demonstrate that a critical difference betw

288 Using mutants of paxillin, we found that dynamic competitive interactions

289 roundabout4 (Robo4) and the adaptor protein, Paxillin were involved in reducing BBB permeability in S

290 at nascent FAs, FAs containing both FAK and paxillin were quantified by image analysis and time corr

291 enuated the activation of MET, Akt, FAK, and paxillin, which are known to regulate adhesion, migratio

292 phosphorylation of focal adhesion kinase and paxillin, which could be restored by re-expression of GI

293 d by the ability of kindlin to directly bind paxillin, which in turn bound focal adhesion kinase (FAK

294 s to enhance the interaction of vinculin and paxillin with each other; however, as with the interacti

295 cificity for the LD1, LD2, and LD4 motifs of paxillin, with K(D) values determined to 27, 42, and 73

296 ia phosphorylated spleen tyrosine kinase and paxillin within focal adhesion complexes.

297 imilarfold decrease in Src Y421 and Y446 and paxillin Y118 was detected, indicating the far-reaching

298 19N and cdc42 T17N, and non-phosphorylatable paxillin Y118/31F and paxillin DeltaLD4 deletion mutants

299 3 inhibited Src-dependent phosphorylation of paxillin (Y118) and downstream activation of Akt (S473),

300 Expression of the paxillin-Y31/118F phosphorylation mutant decreased the C