ライフサイエンスコーパス: penton

1 I loops in three adjacent A subunits in each penton.

2 has been reported to connect the CVSC to the penton.

3 he CVSC density region that is distal to the penton.

4 cts the triplex that is one removed from the penton.

5 27 of UL25 contact the hexon adjacent to the penton.

6 ontact with the triplex one removed from the penton.

7 psid protein subunits of the hexons, not the pentons.

8 ch major capsid protein in hexons but not in pentons.

9 65, binds around the tips of both hexons and pentons.

10 tes with conformational differences in their pentons.

11 o determine the structure of capsids lacking pentons.

12 psids revealed a capsid shell composed of 12 pentons, 150 hexons, and 320 triplexes arranged on a T=1

13 ds are identical and are each composed of 12 pentons, 150 hexons, and 320 triplexes.

14 and blood coagulation factor X (FX), whilst penton-alpha(v)integrin interactions are thought to cont

15 rs and Ad5-based vectors with alterations in penton and fiber was evaluated.

16 The two procapsids consist of very similar penton and hexon clusters, except for an increased curva

17 ossess a T=7 icosahedral symmetry, having 12 pentons and 60 hexons.

18 two of these proteins, VP5 (which forms the pentons and hexons in typical HSV capsids) and VP19C (a

19 of such horn-shaped densities from both the pentons and hexons of the KSHV capsid reconstructions.

20 ssociation of the tegument and VP26 with the pentons and hexons, respectively.

21 (149 kDa), makes up both types of capsomere, pentons and hexons.

22 the divergent tegument binding properties of pentons and hexons.

23 ctive channels present at the centers of the pentons and hexons.

24 Furthermore, these capsids also have lost pentons and peripentonal triplexes as visualized by cryo

25 g the early endosome, mature virions release pentons and peripheral core contents.

26 ting premature disassembly and deployment of pentons and protein VI.

27 ntermediate state, SPs are bound only to MCP pentons and to adjacent subunits from hexons.

28 ontacted both fivefold symmetrical vertices (pentons) and sixfold symmetrical faces (hexons) of the n

29 major adenovirus (Ad) capsid proteins hexon, penton, and fiber influence the efficiency and tropism o

30 by using electron cryomicroscopy, as well as penton- and protein VI-specific antibodies, we show that

31 Differential formations of the hexon and penton are mediated by a drastic alpha-helix-to-beta-str

32 The capsid becomes brittle, and pentons are more easily released.

33 te capsomer shape changes that control where pentons are placed to create normal-sized capsids.

34 d its characteristic nucleocapsid hexons and pentons are preserved.

35 ming the capsid floor between the hexons and pentons, are each composed of one molecule of ORF62 and

36 ies reveal preferential binding of ChAdOx1's penton arginine-glycine-aspartic acid (RGD) motif to the

37 eveals the free intermediate conformation of penton arms, supporting our model for coordinated moveme

38 xons of VP5 (149 kDa) but none on the 12 VP5 pentons at its vertices.

39 by the deletion of the RGD domain in the Ad5 penton base (Ad5DeltaRGD).

40 mponent of the adenoviral type 7 capsid, the penton base (Ad7PB).

41 at function in host cell entry, particularly penton base (PB) proteins.

42 by excess amounts of viral capsid proteins, penton base and fiber during viral replication, can trig

43 ing HAdV induces the complete release of the penton base and fiber proteins as well as a substantial

44 virus chimera, interact with HD5, bridge the penton base and fiber proteins, and provides significant

45 ins (IIIa, VIII, and IX), extensions of the (penton base and hexon) major capsid proteins, and intera

46 vertices, within group-of-six (GOS) tiles--a penton base and its five surrounding hexons.

47 o reveals an altered association between the penton base and the trimeric fiber protein, perhaps refl

48 a hydrophobic ring on the top surface of the penton base and three flexible tails inserted into three

49 etween the trimeric fiber and the pentameric penton base are mediated by a hydrophobic ring on the to

50 hich retains the ability to recognize the Ad penton base as well as vitronectin, an Arg Gly Asp (RGD)

51 proximately 4.2 integrin molecules bound per penton base at close to saturation.

52 Both WOW-1 Fab and penton base bound selectively to activated alpha(V)beta(

53 and its mode of attachment to the pentameric penton base by using an integrative approach of multi-re

54 eptor (CAR) at the cell surface, followed by penton base capsid protein binding to alpha(v) integrins

55 was to detarget Adv uptake through fiber and penton base capsid protein manipulations and determine h

56 d knob with lacrimal acini revealed that the penton base capsid protein remained surface associated,

57 Fiber and penton base capsid proteins of adenovirus type 5 (Ad5) m

58 the crystal structures of both the hexon and penton base capsid proteins.

59 Our recombinant penton base chimera contains polypeptide domains designe

60 The fiber-pseudotyped and penton base constructs with RGD deleted have attributes

61 A global conformational change in penton base could be one step on the way to the release

62 Docking of the hexon and penton base crystal structures into the cryoEM density e

63 The deletion of the RGD motif in the penton base did not affect virus attachment, regardless

64 ckievirus-and-adenovirus receptor (CAR), and penton base facilitates viral internalization by binding

65 nstrated that recombination sites within the penton base gene frame the coding sequences for the two

66 re designed to target a 185-bp region of the penton base gene of HAdV.

67 with HAdV-D19 and HAdV-D37 within the capsid penton base gene.

68 The internal surface of the penton base in Ad2ts1 appears to be anchored to the core

69 could induce a conformational change in the penton base involving clockwise untwisting of the pentam

70 The RGD-containing penton base loops of both the sensitive and resistant vi

71 An effect of the penton base modification was a reduction in the number o

72 evealed a ring of integrin density above the penton base of each virus serotype.

73 d to a cluster of helices observed below the penton base on the inner capsid surface.

74 virus binding could be blocked by a soluble penton base or by a function-blocking monoclonal antibod

75 may function following insertion into either penton base or fiber.

76 pproximately 60 A) of the RGD protrusions on penton base precludes integrin binding in the same orien

77 l arrangement of five RGD protrusions on the penton base promotes integrin clustering and the signali

78 likely due to interactions between the virus penton base protein and integrins.

79 STIV shares the same coat subunit and penton base protein folds as some eukaryotic and bacteri

80 ding RGD (Arg-Gly-Asp) protrusion of the Ad2 penton base protein is highly mobile.

81 -Gly-Asp (RGD) sequence usually found in the penton base protein is important for the interaction of

82 corporated the FLAG peptide epitope into its penton base protein was constructed.

83 Interaction of the adenovirus penton base protein with alpha v integrins promotes viru

84 c cells is facilitated by interaction of the penton base protein with members of the beta2 integrin f

85 IgG antibodies to all five RGD sites on the penton base protein within the intact virus.

86 the five 22 A protrusions on the adenovirus penton base protein.

87 (MAV-1) lacks an RGD sequence in the virion penton base protein.

88 ntry is mediated by interaction of the viral penton base proteins with cellular integrins.

89 us despite both utilizing the same fiber and penton base receptors.

90 ry attachment receptor, it is known that the penton base RGD motifs interact with cellular integrins

91 promoted by the binding of integrins to the penton base RGD sequence, these results suggest that vir

92 t of these novel adenoviruses indicates that penton base RGE mutation in combination with FX binding-

93 ed the interactions between the adenovirus 9 penton base subunit and alphaVbeta3 integrin using fluor

94 Our results indicate that the penton base subunit can bind integrins with high affinit

95 A model with four integrins bound to a penton base suggests that integrin might extend one RGD-

96 nm) permits fiber-independent binding of Ad9 penton base to alpha(v)-integrins.

97 elate with the requirement of the pentameric penton base to simultaneously bind several integrins to

98 However, interaction of the Ad penton base with cell surface integrins contributed to t

99 n extended conformation when bound to the Ad penton base, a multivalent viral ligand.

100 that code for the viral structural proteins, penton base, and fiber.

101 n serve as an alternative to the fiber knob, penton base, and hexon proteins for incorporation of tar

102 Within the penton base, density was observed for the integrin-bindi

103 EKC strains diverged from HAdV-D19p in the penton base, E3, and fiber transcription units.

104 ated into the outer Ad capsid protein hexon, penton base, fiber knob, or protein IX.

105 ation of the interactions of recombinant Ad5 penton base, fiber, and knob with lacrimal acini reveale

106 addition to the three major capsid proteins (penton base, hexon and fiber) and several proteins assoc

107 three major structural capsid proteins, the penton base, hexon, and fiber.

108 revealed that Ad5, fiber, and knob, but not penton base, stimulated macropinocytosis in acini and th

109 D) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR

110 By manipulating fiber and the penton base, we have generated highly detargeted vectors

111 related to human serotype 42, except for the penton base, which is derived from serotype 20.

112 domain, a long flexible shaft domain, and a penton base-attachment tail domain.

113 aled at the base of the hexons and below the penton base.

114 bbon of the variable loop on the side of the penton base.

115 h the m-scFv(HA) when it was introduced into penton base.

116 n-binding domain from multivalent adenovirus penton base.

117 ut was blocked with a combination of F9K and penton base.

118 calized reconstructions of unique hexons and penton base.

119 calized reconstructions of unique hexons and penton base.

120 le grooves formed by neighboring subunits of penton base.

121 nterpreted due to symmetry mismatch with the penton base.

122 etrating activities of the adenovirus capsid penton base.

123 fiber attaches to its underlying pentameric penton base.

124 um of four integrins fit over the pentameric penton base.

125 ution (27 A) for integrin density above each penton base.

126 terminal region of the fiber) but not excess penton base.

127 Flexible penton-base RGD loops and incoherent averaging of bound

128 the transporter complex the appearance of a penton-based pyramid.

129 lycine-aspartate (RGD) domain present in the penton bases of the capsids.

130 The portal vertex appears different from pentons, being located partially inside the capsid shell

131 n be divided into three prominent regions: a penton-binding globular region, a helix-bundle stalk reg

132 between the tegument and capsid involves the pentons but not the hexons.

133 We propose that Ad7 pentons can be developed into integrin-specific gene del

134 psids with and without UL37 reveals the same penton-capping density in both cases.

135 eins in regulating DNA transport through the penton channel and binding to cellular transport protein

136 host cell nucleus, with the exception of the penton channel, which is closed.

137 capsid contains a scaffolding core, and its penton channels are closed.

138 The A capsid is empty, and its penton channels are open.

139 spaced density shells (25 A apart), and its penton channels are open.

140 The different statuses of the penton channels suggest a functional role of the channel

141 e apparent inside the shells and through the penton channels.

142 The adenovirus capsid protein penton contains a well characterized arginine-glycine-as

143 HAdV3 produces large amounts of penton-dodecahedra (PtDd), virus-like particles, during

144 on the capacity of HAdV3 to produce subviral penton-dodecahedral particles that act as decoys for HD5

145 s usual sites on hexons but does not bind to pentons, even when available in 100-fold molar excess.

146 ere used to detect signatures for the hexon, penton, fiber, minor capsid, and core proteins.

147 t that they may have roles in DNA packaging, penton formation, and protein-protein interaction.

148 articles are well described by the A subunit pentons from TBSV.

149 The loss of the pentons further suggests the necessity of specific pento

150 s in the three distinct morphological units: penton, hexon, and triplex of this T = 16 icosahedral pa

151 We propose a model whereby the penton-hexon interactions are stabilized through interac

152 s further suggests the necessity of specific penton-hexon interactions during expansion.

153 ciated tegument complexes (CATC): SCPs crown pentons/hexons and mediate tegument protein binding, and

154 the virus fiber (site of host cell binding), penton (host cell internalization signal), hexon (princi

155 internal protein VI is required to stabilize pentons in the particle while coinciding with penton rel

156 s bound to the tips of the hexons but not to pentons, indicating that KSHV SCP is attached to the upp

157 e step of virus infection and indicates that penton-integrin interactions are involved in internaliza

158 Furthermore, the role of penton-integrin interactions remains unknown for new vec

159 sessing mutations ablating both hexon:FX and penton:integrin interactions.

160 appears as filamentous structures around the pentons, interacting extensively with the capsid.

161 d stability, and especially the retention of pentons, is regulated by the formation of disulfide bond

162 Hexons expand first, and we propose penton maturation is delayed by a symmetry match with SP

163 these subunits in the capsid, fibers engaged pentons more frequently than hexons.

164 the in vivo expanded capsid except that the pentons, normally present at the icosahedral fivefold po

165 As with HSV-1, the hexons but not the pentons of the major capsid protein (151 kDa) bind the s

166 ibody binds only to the pentameric clusters (pentons) of A subunits of the T=3 quasisymmetric virus a

167 model for coordinated movement of hexons and pentons on the capsid lattice.

168 f the major capsid protein, 60 copies of the penton protein and 1800 minor capsid proteins of which t

169 -hexameric morphology, and of 60 copies of a penton protein at the vertices.

170 a dose of 38 mJ/cm(2) reduced the hexon and penton protein quantities to approximately 33% and 31% o

171 to five major capsid protein variants and a penton protein variant.

172 and prediction, we elucidated ORF142 as the penton protein, which plugs the 12 vertices of the capsi

173 minor capsid protein of Bam35 and P24 is the penton protein, with the latter finding also being suppo

174 Hexon and penton proteins exposed to a dose of 28 mJ/cm(2) emitted

175 eatment of capsids, our study indicates that penton release may be a common trend among double-strand

176 entons in the particle while coinciding with penton release upon entry and that release of protein VI

177 y, pseudotyping with the HAdV-5 fiber and/or penton RGD loop had little to no effect on in vivo trans

178 However ablation of the penton RGD motif in the AdT* vector background (AdT*RGE)

179 This study also demonstrates the role of penton RGD motifs in facilitating the endosome escape st

180 capsid protein in hexons but not to that in pentons, similar to HSV-1 SCP.

181 UL25 dimer in alphaherpesviruses, binds each penton subunit, an observation that correlates with conf

182 eak density that extends the CVSC toward the penton, suggesting either that this extra density is for

183 60 capsomers are hexons, while the rest are pentons that are correctly positioned during assembly.

184 f weak but significant density shrouding the pentons that we attribute to tegument proteins.

185 rowding of the subunits at the centre of the pentons, thereby favouring the hexon configuration durin

186 l triplexes, but notably only about one peri-penton triplex.

187 s, with five tegument densities capping each penton vertex, a pattern highly similar to that in alpha

188 ly stabilize the hexon shell, thus rendering penton vertices the weakest links of the adenovirus caps

189 Quasi-equivalence requires that penton VP5 must differ in conformation from hexon VP5: o

190 The penton was expressed in Escherichia coli as a soluble C-

191 When this region of the penton was mutated, TNF-alpha expression and bone marrow

192 er except around the bases of the hexons and pentons, whereas they are tightly clustered into capsome

193 density as the region that is distal to the penton with the N-terminus of UL25 making contact with t