ライフサイエンスコーパス: pericentrin

1 psies, whereas normal tissues exhibit intact pericentrin.

2 ction to the central region of both LIC1 and pericentrin.

3 Only the LICs coimmunoprecipitated with pericentrin.

4 ent cells that was suppressed by the loss of pericentrin.

5 ma-TuRC fractions did not contain detectable pericentrin.

6 s using antibodies to the centrosome protein pericentrin.

7 e, extracentriolar foci of gamma-tubulin and pericentrin.

8 sponding site (ALRRLLD948-L949FGD) in murine pericentrin.

9 MMP targets an integral centrosomal protein, pericentrin.

10 er, our results confirm that MT1-MMP cleaves pericentrin-2 in humans but not in mice and that mouse m

11 ly important, integral, centrosomal protein, pericentrin-2, was a cleavage target of MT1-MMP in human

12 and cleaved the integral centrosomal protein pericentrin-2.

13 ffect of PKCbetaII inhibition on normalizing pericentrin (a protein regulating cytokinesis), especial

14 ation and function, in part through elevated Pericentrin, a centrosome protein encoded on chromosome

15 entrally located site which colocalized with pericentrin, a component of the MTOC.

16 Pericentrin, a critical centrosome component first ident

17 rin share significant sequence homology with pericentrin, a previously identified murine centrosome c

18 Proteins that contain a Pericentrin/AKAP450 centrosomal targeting (PACT) domain

19 Immunoprecipitation of endogenous pericentrin also pulled down cytoplasmic dynein in untra

20 Here, we show that pericentrin, an integral component of the pericentriolar

21 We conclude that pericentrin anchoring of gamma tubulin complexes at cent

22 how that the centrosomal coiled-coil protein pericentrin anchors gamma TuRCs at spindle poles through

23 rosome assembly and induced modifications of pericentrin and centrin-2, two essential proteins requir

24 From these studies we conclude that pericentrin and gamma tubulin are novel dynein cargoes t

25 Centrosomes in cells with reduced levels of pericentrin and gamma tubulin have a diminished capacity

26 cent protein particles containing endogenous pericentrin and gamma tubulin move along microtubules at

27 proteins involved in microtubule nucleation, pericentrin and gamma tubulin, is inhibited in the absen

28 Pericentrin and gamma-tubulin are integral centrosome pr

29 Our results indicate that pericentrin and gamma-tubulin assemble into a unique cen

30 duces the loss of the pericentriolar markers pericentrin and gamma-tubulin from centrosomes.

31 ut not CHD4, by RNA interference dissociated pericentrin and gamma-tubulin from centrosomes.

32 ome component based its co-localization with pericentrin and gamma-tubulin within microtubule organiz

33 ity were important for proper recruitment of pericentrin and gamma-tubulin, and, ultimately, for form

34 ent of pericentriolar material, specifically pericentrin and gamma-tubulin, to the centrosome.

35 rganizing center (MTOC)-associated proteins, pericentrin and gamma-tubulin.

36 These analyses demonstrate that pericentrin and kendrin are encoded by one gene, correct

37 eveal that chromosome 21 polyploidy elevates Pericentrin and microtubules away from the centrosome th

38 ion reduced the level and mislocalization of pericentrin and normalized microtubule organization in t

39 ditions associated with loss or elevation of pericentrin and propose cellular and molecular models th

40 -expressed MT1-MMP with the wild type murine pericentrin and the D948G mutant.

41 ween the mouse chromosome 10 region encoding pericentrin and the human chromosome 21 region encoding

42 letion abolishes the ability of Chk1 to bind pericentrin and to localize at centrosomes, which, in it

43 ree centrosomal antigens (hsp 73, TCP-1, and pericentrin) and the recovery of microtubule regrowth pr

44 of PCM components, including gamma-tubulin, pericentrin, and Cdk5Rap2, with centrosomes exhibiting r

45 C1 and LIC2 for the ability to interact with pericentrin, and found that only LIC1 will bind.

46 roaches led to reduced targeting of centrin, pericentrin, and ninein to the centrosome.

47 red the ability to activate MMP-2, to cleave pericentrin, and to invade the Matrigel matrix.

48 ere evaluated with respect to gamma-tubulin, pericentrin, and total tubulin polymer fractions at spec

49 Both Cdc2 and pericentrin antibodies decorate the amorphous pericentri

50 material (PCM) containing gamma-tubulin and pericentrin appear in the cell.

51 Cytoplasmic dynein, dynactin, and pericentrin are all recruited to the interphase aMTOC, a

52 organizing center proteins gamma-tubulin and pericentrin, are major sites of muscle MT nucleation, in

53 of FTIs on centrosome separation and define pericentrin as a (indirect) target of FTIs affecting cen

54 creen, we identified the centrosomal protein pericentrin as a scaffold that tethers PKC betaII to cen

55 in B1 and proteasome-mediated degradation of pericentrin as critical components in FTI-induced "donut

56 An interaction between pPKCdelta(Thr505) and pericentrin as well as gamma-tubulin was confirmed by co

57 ein, only the former of which is involved in pericentrin association with dynein.

58 function is mediated by an interaction with pericentrin at centrosomes.

59 that endogenous PKC betaII colocalizes with pericentrin at centrosomes.

60 ifically co-localizes with gamma-tubulin and pericentrin at microtubule-organizing centers (MTOCs) in

61 Studies on pericentrin at the cellular, molecular, and, more recent

62 of centriole assembly or genetic ablation of pericentrin attenuated interleukin-6, interleukin-10, an

63 Overexpression of the pericentrin-binding domain of CHD4 or another family mem

64 ganizing center containing gamma-tubulin and pericentrin, but did not regenerate centrioles.

65 ow that many NuRD components interacted with pericentrin by coimmunoprecipitation and that they local

66 Mutations in pericentrin cause the human genetic disorder Majewski/mi

67 rin (PCNT), encoding the centrosomal protein pericentrin, cause a form of osteodysplastic primordial

68 of PCM components depends on gamma-tubulin, pericentrin, CDK5RAP2 and ninein, but not NEDD1, CEP152,

69 uence, and the 3' end of the published mouse pericentrin cDNA is a fragment from a different mouse ch

70 he stop codon present in the published mouse pericentrin cDNA is not found in the mouse genomic seque

71 However, comparison of the published mouse pericentrin cDNA sequence to mouse genomic DNA sequences

72 y one gene, correct the previously published pericentrin cDNA sequence, and describe the complex expr

73 proteins to MTNCs, including gamma-tubulin, pericentrin, Cep68, Cep170, and Cdk5RAP2.

74 , we show that in the absence of galectin-8, pericentrin compactness is lessened and mitotic microtub

75 exes with CHD3 and CHD4, but a distinct CHD3-pericentrin complex is required for centrosomal anchorin

76 ut MTOC compaction requires cooperation with pericentrin-containing self-clustering PCM.

77 Through these interactions, pericentrin contributes to a diversity of fundamental ce

78 differentiation may contribute to this, but pericentrin deficiency does not impair proximal insulin

79 Furthermore, pericentrin deficiency or mutation of a separase cleavag

80 level of PCM expansion, with a reduction in pericentrin-deficient or separase cleavage site mutant-e

81 protein remains associated with MTOCs, in a pericentrin dependent manner.

82 A and Plk1, targets them to centrosomes in a pericentrin-dependent manner, and promotes sequential ac

83 M to the nuclear DDR in which CHK1 regulates pericentrin-dependent PCM expansion to control its own a

84 ith PCNT defects and examined the effects of pericentrin depletion on insulin action using 3T3-L1 adi

85 To confirm that MT1-MMP itself cleaves pericentrin directly, rather than indirectly, we analyze

86 Yet how trisomy 21 and elevated Pericentrin disrupt cilia-related molecules and pathways

87 erexpression of the GCP2/3 binding domain of pericentrin disrupted the endogenous pericentrin-gamma T

88 ogical role for LIC, and they suggest that a pericentrin-dynein interaction in vivo contributes to th

89 ells that transiently or permanently express pericentrin exhibit severe centrosome and spindle defect

90 Pericentrin expression and localization also was determi

91 Moreover, both gamma-tubulin and pericentrin expression at MTOCs were decreased in pPKCde

92 Pericentrin expression in human tissues was profiled usi

93 Reducing pericentrin expression or ectopic expression of nonfarne

94 onstrated by continuing Ad localization with pericentrin for more than 5 h after infection, a strong

95 We conclude that pericentrin forms complexes with CHD3 and CHD4, but a di

96 or another family member (CHD3) dissociated pericentrin from centrosomes.

97 pericentrin (PCNT)--resulting in the loss of pericentrin from the centrosome, where it has key functi

98 Our work reveals that elevated Pericentrin from trisomy 21 disrupts multiple early step

99 ide a developmental basis for association of pericentrin function with interneuron defects in human s

100 To better understand pericentrin function, we overexpressed the protein in so

101 cells expressing a green fluorescent protein-pericentrin fusion protein, green fluorescent protein pa

102 e microtubule nucleation-organizing proteins pericentrin, gamma-tubulin, and Cdk5Rap2, and microtubul

103 The acentrosomal pole lacks pericentrin, gamma-tubulin, and centrioles, however.

104 main of pericentrin disrupted the endogenous pericentrin-gamma TuRC interaction and perturbed astral

105 The pericentrin-gamma-tubulin complex was distinct from the

106 lex is required for centrosomal anchoring of pericentrin/gamma-tubulin and for centrosome integrity.

107 ive northern blot analysis revealed that the pericentrin gene displays a complex expression pattern i

108 Although pericentrin has been known to be up-regulated in epithel

109 a mouse model of Down syndrome with elevated Pericentrin has fewer primary cilia in cerebellar granul

110 ed in fission yeast by overexpression of the pericentrin homolog Pcp1p.

111 d that PKA catalytic activity is enriched in pericentrin immunoprecipitates.

112 Knockdown of pericentrin in adipocytes had no effect on proximal insu

113 o-localizes with gamma-tubulin, centrin, and pericentrin in centrosomes.

114 Depletion of pericentrin in neural progenitors phenocopies effects of

115 y elevating levels of the centrosome protein pericentrin in prostate epithelial cell lines reproduces

116 MT1-MMP was colocalized with pericentrin in the centrosomal compartment and especiall

117 of MT1-MMP activity correlates with degraded pericentrin in tumor biopsies, whereas normal tissues ex

118 teins, pericentriolar material 1 (PCM-1) and pericentrin, in primary human fibroblasts.

119 evealed that a conserved PCM component, Pcp1/pericentrin, interacts with and recruits SAS-6.

120 centrosomal recruitment of gamma-tubulin and pericentrin, interferes with microtubule organization, d

121 Pericentrin is a conserved protein of the centrosome inv

122 Pericentrin is a critical centrosomal protein required f

123 , these results provide strong evidence that pericentrin is an AKAP in vivo.

124 Pericentrin is an integral centrosomal component that an

125 Pericentrin is an integral component of the centrosome t

126 conserved calmodulin-binding region of Pcp1/pericentrin is critical for SAS-6 interaction.

127 Pericentrin is known to be essential to the normal funct

128 Whereas the localization of pericentrin is not affected, alpha- and gamma-tubulin lo

129 These findings indicate that pericentrin is required for proper migration of olfactor

130 Additionally, overexpressed pericentrin is seen to interact with endogenous LIC1 exc

131 We found that pericentrin is up-regulated in human tumor endothelium c

132 ytic subunit of PKA coimmunoprecipitate with pericentrin isolated from HEK-293 cell extracts and that

133 evels of Pcp1, the fission yeast ortholog of pericentrin/kentrin, and reducing pcp1(+) expression sig

134 The effect of pericentrin knockdown on insulin action and adipogenesis

135 Cdc2 and to the centrosome-specific protein, pericentrin, label the centrosome in an apparently cell

136 Our study identifies a role of the TRIM43-pericentrin-lamin axis in intrinsic immunity, which may

137 hog signaling in direct anticorrelation with Pericentrin levels.

138 Here we show that fission-yeast pericentrin-like Pcp1 regulates multiple functions of th

139 This mechanism relies on the restriction of pericentrin-like protein (PLP) and the pericentriolar ma

140 We identify pericentrin-like protein (PLP) as a negative regulator o

141 Drosophila Pericentrin-like protein (PLP) directs formation of a pe

142 We identify an unprecedented role for Pericentrin-like protein (PLP), which localizes to the t

143 domain protein in Drosophila (the Drosophila pericentrin-like protein [D-PLP]) is associated with bot

144 We identify Pericentrin-Like-Protein (PLP) as a novel Kinesin-1 inte

145 Here we use Drosophila pericentrin-like-protein (PLP) to understand how the PAC

146 ed Gle1 levels are correlated with decreased pericentrin localization at the centrosome and microtubu

147 and dynactin, are lost from centrosomes, but pericentrin localization persists.

148 ction to the pericentriolar material protein pericentrin, loss of function of which is associated wit

149 the complexity of phenotypes associated with pericentrin-mediated disorders is somewhat daunting, ins

150 l phenotypes were significantly reduced in a pericentrin mutant with diminished GCP2/3 binding and we

151 Pericentrin-null mutants also exhibited defects in leftw

152 Laser ablation of mitotic cells and pericentrin-null mutants, both reducing mitotic events,

153 entrioles but cannot assemble gamma-tubulin, pericentrin, or other pericentriolar proteins into an or

154 includes the proteins Spc42, Spc110 (kendrin/pericentrin ortholog), calmodulin (Cmd1), Spc29, and Cnm

155 Spindles in pericentrin-overexpressing cells were disorganized and m

156 Like pericentrin, p-MARCKS staining at the MI spindle poles w

157 The large coiled-coil protein, pericentrin, participates in PCM assembly and has been i

158 alization of centrosomal proteins, including Pericentrin, Pcm1, and gamma-tubulin, depends on Nesprin

159 Here, we show that the centrosome protein pericentrin (Pcnt) colocalizes with IFT proteins to the

160 t and premature termination mutations in the pericentrin (PCNT) gene were identified in individuals w

161 we identified a frame shift mutation in the pericentrin (Pcnt) gene.

162 As in MOPDII patients, disruption of pericentrin (Pcnt) in mice caused a number of abnormalit

163 s caused by mutations in the centrosome gene pericentrin (PCNT) that lead to severe pre- and postnata

164 Genetic defects in human pericentrin (PCNT), encoding the centrosomal protein per

165 w report that mutations in the gene encoding pericentrin (PCNT)--resulting in the loss of pericentrin

166 ), myosin IIB (MYOIIB), aPKCzeta, LGL, PAR3, pericentrin, PROM1] is lost.

167 The centrosomal pericentrin-related proteins play pivotal roles in vario

168 n by expression of the interacting region of pericentrin results in release of PKC from the centrosom

169 corresponds to the 3' end for a 7.1-kb mouse pericentrin RNA encoded on chromosome 10.

170 Germaine to pericentrin's function in organizing PCM is its ability

171 We found that cells depleted of PCM-1 or pericentrin show lower levels of markers for S phase and

172 Pericentrin silencing by small interfering RNAs in somat

173 Additionally, pericentrin silencing or overexpression induced G2/antep

174 Yeast pericentrin, Spc110, binds to and activates the gamma-tu

175 as confirmed by (i) the assembly of multiple pericentrin-stained centrioles at the apical surface, (i

176 In addition, virtually all pericentrin-staining structures in tumor cells nucleated

177 nein coimmunoprecipitated with overexpressed pericentrin, suggesting that the motor was sequestered i

178 TRIM43 ubiquitinates the centrosomal protein pericentrin, thereby targeting it for proteasomal degrad

179 Recent studies link pericentrin to a growing list of human disorders.

180 nt with these observations is the ability of pericentrin to cosediment with taxol-stabilized microtub

181 n that recruits Spc110, a protein related to pericentrin, to the SPB.

182 To define the basis for this interaction, pericentrin was coexpressed with cytoplasmic dynein heav

183 Pericentrin was highly expressed in human skeletal muscl

184 recently identified, but its relationship to pericentrin was not clear.

185 sitive to MT1-MMP, whereas unmodified murine pericentrin was resistant to proteolysis.

186 hat exhibited the cleavage sequence of human pericentrin was sensitive to MT1-MMP, whereas unmodified

187 In a yeast two-hybrid screen with pericentrin we identified chromodomain helicase DNA-bind

188 along with the centrosome-specific protein, pericentrin, were also present within an enriched prepar

189 he LICs: binding to the centrosomal protein, pericentrin, which represents a novel, non-dynactin-base

190 somal proteins, including Akap450, Pcm1, and Pericentrin, whose association with Nesprin-1alpha is in

191 The interaction of pericentrin with RII is mediated through a binding domai

192 ed by the C1A domain of PKC and a segment of pericentrin within residues 494-593.