ライフサイエンスコーパス: perilipin

1 ts major lipid droplet-associated substrate, perilipin.

2 calizes with the known lipid droplet protein perilipin.

3 PGC-1alpha and UCP-2, and down-regulation of perilipin.

4 ogue of the vertebrate lipid-storage protein perilipin.

5 phosphorylating hormone-sensitive lipase and perilipin.

6 e major lipid storage site and is defined by perilipin.

7 cilitate the PKA-mediated phosphorylation of perilipin.

8 requires the phosphorylation of both HSL and perilipin.

9 se of the loss of the protective function of perilipin.

10 xpressed in 3T3-L1 preadipocytes, which lack perilipins.

11 -length perilipin A or control cells lacking perilipins.

12 ctions of ATGL and its co-lipase CGI-58 with perilipin 1 (perilipin A), perilipin 2 (adipose differen

13 cytosolic hormone-sensitive lipase (HSL) and perilipin 1 (Plin1) in the lipid droplet by protein kina

14 se (FAS), hormone sensitive lipase (HSL) and perilipin 1 (PLIN1), was examined in the present study.

15 O-GlcNAcylation of lipid droplet-associated perilipin 1 (PLIN1), which leads to elevated PLIN1 phosp

16 Phosphorylation of perilipin 1 disrupts these interactions and mobilizes CG

17 in the liposarcoma cell line LiSa-2 restored perilipin 1 expression, as exhibited in the source tumor

18 Furthermore, knockdown of perilipin 1 in adipocytes leads to replacement of perili

19 on affecting the carboxy-terminus (439fs) of perilipin 1 in two unrelated families.

20 Perilipin 1 is a lipid droplet coat protein predominantl

21 In summary, perilipin 1 is a promising marker for the differential d

22 In contrast, neither perilipin 1 nor 2 interacted directly with ATGL.

23 Collectively these data suggest that whereas perilipin 1 potently suppresses basal lipolysis in adipo

24 mplementation studies suggested that whereas perilipin 1 prevents the activation of adipose tissue tr

25 ns between CGI-58 and the LD coating protein perilipin 1 restrain the ability of CGI-58 to activate A

26 served region within the carboxy terminus of perilipin 1 that binds and stabilizes ABHD5 by retarding

27 generated by fusing the carboxy terminus of perilipin 1 to the amino terminus of perilipins 2 or 3 s

28 Perilipin 1 was immunohistochemically positive in all st

29 o ubiquitinated proteins, possibly including perilipin 1, on LDs.

30 The discovery by Dr. Constantine Londos of perilipin 1, the major scaffold protein at the surface o

31 Perilipin 1, the most abundant lipid-coat protein in adi

32 omas were immunohistochemically positive for perilipin 1.

33 es of soft tissue sarcomas were negative for perilipin 1.

34 basal lipolysis as effectively as wild-type perilipin 1.

35 ssues express perilipins 2 or 3, rather than perilipin 1.

36 We designate PPE15 as mycobacterial perilipin-1 (MPER1).

37 ) interacts with perilipin-5 (Plin5) but not perilipin-1 (Plin1).

38 mino acid sequence identities with mammalian perilipin-1 and was upregulated in Mtb dormancy.

39 l transporter aquaporin 7 and phosphorylated perilipin-1 following adrenergic stimulation.

40 FXR upregulates Perilipin-1, a direct target gene of FXR, to stabilize l

41 ipase CGI-58 with perilipin 1 (perilipin A), perilipin 2 (adipose differentiation-related protein), a

42 (adenovirus [Ad]-PLIN5) and those expressing perilipin 2 (PLIN2) (Ad-PLIN2) had higher [(3)H]FA incor

43 ivity are related to increased expression of perilipin 2 (PLIN2) and perilipin 5 (PLIN5).

44 Perilipin 2 (Plin2) is a LD protein that is involved in

45 Perilipin 2 (PLIN2) is a lipid-droplet protein that is u

46 usly reported that the lipid droplet protein perilipin 2 (PLIN2) modulates lipid homeostasis in the l

47 ipose differentiation-related protein (Adrp)/perilipin 2 (Plin2), and investigated its effects on ath

48 influence on the expression of adipophilin [perilipin 2 (PLIN2)], a well-known PPARgamma target.

49 Perilipin 2 (PLIN2/adipose differentiation-related prote

50 mas from other soft tissue sarcomas, whereas perilipin 2 correlates negatively with tumor grade and m

51 ipin 1 in adipocytes leads to replacement of perilipin 2 on LDs.

52 Furthermore, knockdown of perilipin 2 or perilipin 3 in LiSa-2 cells influenced li

53 Perilipin 2 was more prominent in dedifferentiated and p

54 sarcomas, lipomas, or normal adipose tissue, perilipin 2 was virtually absent.

55 thase, stearoyl-coenzyme A desaturase 1, and perilipin 2) was drastically reduced by EGFR inhibition.

56 In contrast, perilipin 2, a lipid droplet-stabilizing protein, is pro

57 Perilipin 2, which coats lipid droplets, is markedly ind

58 that the sequestration of hepatic lipids in perilipin 2-coated droplets ameliorates insulin resistan

59 ty acid, oleic acid, induces accumulation of perilipin 2-coated lipid droplets containing triglycerid

60 r, AB-hydrolase domain containing-5 (ABHD5), perilipins 2 and 3 do so less effectively.

61 ly suppresses basal lipolysis in adipocytes, perilipins 2 and 3 facilitate higher rates of basal lipo

62 We sought to understand the role of perilipins 2 and 3 in regulating basal lipolysis.

63 inus of perilipin 1 to the amino terminus of perilipins 2 or 3 stabilize ABHD5 and suppress basal lip

64 These tissues express perilipins 2 or 3, rather than perilipin 1.

65 ess basal lipolysis more effectively than WT perilipins 2 or 3.

66 Four of the five LD coat proteins, including perilipins 2, 3, 4, and 5, were increased in the CGI-58

67 ained abundant lipid droplets, LD-associated perilipins 2/3/5, hormone-sensitive lipase, and 1-acylgl

68 ion and organization of LD scaffold proteins perilipin-2 (PLIN2) and perilipin-5 (PLIN5).

69 specificity of urine aquaporin-1 (AQP1) and perilipin-2 (PLIN2) concentrations as unique, noninvasiv

70 Perilipin-2 (PLIN2) is a constitutively associated cytop

71 patocyte-specific or whole-body deletions of perilipin-2 (Plin2) were used to define hepatocyte and e

72 Perilipin-2 (Plin2), a ubiquitously expressed cytoplasmi

73 Mice with or without whole-body deletion of perilipin-2 (Plin2-null) were fed either Western or cont

74 ther verified by the increased expression of perilipin-2 and decreased activity of hormone-sensitive

75 Perilipin-2 deletion prevents obesity and insulin resist

76 reased lipid droplet accumulation, increased perilipin-2 expression, and decreased HSL activity.

77 converting it to triacylglycerol, attenuated perilipin 3 DG-induced ER recruitment.

78 ing trafficking with AlF(4)(-) redistributed perilipin 3 differently under conditions of triacylglyce

79 Furthermore, knockdown of perilipin 2 or perilipin 3 in LiSa-2 cells influenced lipid droplet num

80 he DG lipase inhibitor RHC80267 enhanced the perilipin 3 recruited to lipid droplets and raised DG le

81 ion with triacsin C, enhanced recruitment of perilipin 3 to the ER.

82 cells with a membrane-permeable DG recruited perilipin 3 to the ER.

83 acid-induced triacylglycerol synthesis drove perilipin 3 to the tubular ER.

84 in 3, but when added together with AlF(4)(-) perilipin 3 was recruited to lipid droplets rather than

85 ates adenylate cyclase, did not redistribute perilipin 3, but when added together with AlF(4)(-) peri

86 We show that perilipin 3- and perilipin 4-coated lipid droplets emerg

87 rolytic product, affects the distribution of perilipin 3.

88 l droplets with a protein coat that includes perilipin 3/TIP47 and perilipin 4/S3-12.

89 ting protein of 47 kDa (TIP47; also known as perilipin-3 and mannose-6-phosphate receptor-binding pro

90 Membrane-permeable DG also drove perilipin 4 and 5 onto the ER.

91 We show that perilipin 3- and perilipin 4-coated lipid droplets emerge along the endop

92 ein coat that includes perilipin 3/TIP47 and perilipin 4/S3-12.

93 dipose differentiation-related protein), and perilipin 5 (LSDP5) using multiple techniques as follows

94 r hypothesis that lipid droplet (LD) protein perilipin 5 (PLIN5) in beta-cells aids PP insulin secret

95 physiological studies related to the role of perilipin 5 (Plin5) in regulating lipid droplet accumula

96 Perilipin 5 (PLIN5) is a lipid droplet protein and is hi

97 trol of intracellular fatty acid fluxes, and perilipin 5 (PLIN5) is important in this process.

98 M, trained athletes possess higher levels of perilipin 5 (PLIN5), a lipid droplet (LD) coating protei

99 Moreover, we characterize the LD protein perilipin 5 (PLIN5), which is known to enhance mitochond

100 reased expression of perilipin 2 (PLIN2) and perilipin 5 (PLIN5).

101 tients, Trained subjects have high levels of perilipin 5 (PLIN5).

102 Cells expressing both ectopic perilipin 5 and ATGL showed a 3-fold increase in lipolys

103 Here we identify the lipid droplet protein Perilipin 5 as a catecholamine-triggered interaction par

104 Our studies establish perilipin 5 as a novel ATGL partner and provide evidence

105 sed [(32)P]orthophosphate incorporation into perilipin 5 by 2-fold, whereas neither ATGL nor CGI-58 w

106 lipolysis, whereas interaction of ATGL with perilipin 5 decreased lipolysis.

107 improper delivery, potentially via decreased perilipin 5 expression and mitochondrial-LD tethering, a

108 We propose that Perilipin 5 is an important molecular link that couples

109 t during catecholamine-stimulated lipolysis, Perilipin 5 is phosphorylated by protein kinase A and fo

110 Perilipin 5 promotes PGC-1alpha co-activator function by

111 ss of function studies in cells that nuclear Perilipin 5 promotes transcription of genes that mediate

112 ript levels for the mitochondrial-LD tether, perilipin 5, in the failing myocardium (P = 0.003).

113 lts show that ATGL interacts with CGI-58 and perilipin 5; the latter is selectively expressed in oxid

114 se triglyceride lipase (Atgl) interacts with perilipin-5 (Plin5) but not perilipin-1 (Plin1).

115 LD scaffold proteins perilipin-2 (PLIN2) and perilipin-5 (PLIN5).

116 The expression of OXPAT/perilipin-5, adipose triglyceride lipase, and stearoyl-C

117 tion of the lipid droplet-associated protein perilipin A (Peri A) mediates the actions of cyclic AMP-

118 kinase A (PKA)-dependent phosphorylation of perilipin A (Peri A), an essential lipid droplet (LD)-as

119 location of hormonesensitive lipase (HSL) to perilipin A (Plin)-containing droplets and increases the

120 d lipase, we conclude that the expression of perilipin A alone is sufficient to confer PKA-mediated l

121 Full-length perilipin A associates with lipid droplets via hydrophob

122 hydrolysis in adipocytes; phosphorylation of perilipin A by protein kinase A facilitates maximal lipo

123 Perilipin A coats the lipid storage droplets in adipocyt

124 A peptide composed of the central domain of perilipin A directed a fused green fluorescent protein t

125 lipid droplet and cytoplasmic pools, whereas perilipin A does not.

126 A-mediated phosphorylation of serine 492 of perilipin A drives the fragmentation and dispersion of l

127 but activation of PKA and phosphorylation of perilipin A engenders a 7-fold lipolytic activation.

128 FP or LSDP5; in contrast, phosphorylation of perilipin A exerts the major control over HSL-mediated l

129 d droplets, we stably expressed fragments of perilipin A in 3T3-L1 fibroblasts.

130 The precocious expression of full-length perilipin A in 3T3-L1 preadipocytes aided more rapid sto

131 oxyl termini are critical to the function of perilipin A in facilitating triacylglycerol storage.

132 e roles of the amino and carboxyl termini of perilipin A in facilitating triacylglycerol storage.

133 Perilipin A inhibits triacylglycerol hydrolysis by 87% w

134 Perilipin A is a key regulator of triacylglycerol storag

135 inal protein kinase A consensus sequences of perilipin A is required for HSL binding and maximal lipo

136 dicate that the unique C-terminal portion of perilipin A is responsible for its protection against li

137 Perilipin A is the most abundant lipid droplet-associate

138 droplets coated with perilipin B or mutated perilipin A lacking this sequence.

139 ed with that of cells expressing full-length perilipin A or control cells lacking perilipins.

140 CGI-58 binds to lipid droplets coated with perilipin A or mutated forms of perilipin with an intact

141 hus, we conclude that the central 25% of the perilipin A sequence contains all of the amino acids nec

142 previously shown that the central region of perilipin A targets and anchors it to lipid droplets, at

143 The mutation of serine 492 of perilipin A to alanine prevented the dispersion of clust

144 that the sequences responsible for anchoring perilipin A to lipid droplets are most likely domains of

145 To map the domains that target and anchor perilipin A to lipid droplets, we stably expressed fragm

146 the carboxyl terminus is required to target perilipin A to lipid droplets; however, there are multip

147 protein kinase A-mediated phosphorylation of perilipin A triggers the remodeling of lipid droplets.

148 erminus of 112 amino acids that is unique to perilipin A were critical to facilitate triacylglycerol

149 boxyl-terminal truncation mutations of mouse perilipin A were stably expressed in 3T3-L1 preadipocyte

150 substitution of serines 433, 492, and 517 of perilipin A with glutamic acid residues blocked the disp

151 L and its co-lipase CGI-58 with perilipin 1 (perilipin A), perilipin 2 (adipose differentiation-relat

152 the mechanisms by which PAT family members, perilipin A, adipose differentiation-related protein (AD

153 ipid accumulation (hormone-sensitive lipase, perilipin A, and LDs).

154 ltured fibroblasts stably expressing ectopic perilipin A, clustered lipid droplets disperse throughou

155 in cells that express fully phosphorylatable perilipin A, confirming that perilipin is required to el

156 amster ovary cells that express both HSL and perilipin A, these two proteins cooperate to produce a m

157 Thus, CGI-58 binds to perilipin A-coated lipid droplets in a manner that is de

158 d droplets to fragment into myriad dispersed perilipin A-covered microlipid droplets.

159 roduction of GFP-tagged HSL with and without perilipin A.

160 both protein kinase A-phosphorylated HSL and perilipin A.

161 creased expression of cytoplasmic lipids and perilipin A.

162 major splice variants of the perilipin gene, perilipins A and B, in Chinese hamster ovary fibroblasts

163 investigated the intracellular targeting of perilipin, a major lipid coat protein, and hormone-sensi

164 tein, colocalizes around lipid droplets with perilipin, a regulator of lipolysis.

165 of PKA sites within the N-terminal region of perilipin abrogates the PKA-mediated lipolytic response.

166 mbryonic fibroblasts stably transfected with perilipin accumulated approximately 4.5-fold less lipid

167 lipid droplet-associated PAT protein family (perilipin, ADFP, and Tip47).

168 by associated proteins of the perilipin/PAT (perilipin, adipophilin, and tail-interacting protein of

169 Lipid droplet proteins of the PAT (perilipin, adipophilin, and TIP47) family regulate cellu

170 receptor-binding protein 1), a member of the perilipin, adipophilin, TIP47 (PAT) family of proteins i

171 olutionarily related family of PAT proteins (Perilipin, Adipophilin, TIP47), which is defined by sequ

172 lly contains at least one member of the PAT (perilipin, adipose differentiation-related protein, and

173 LDs are coated with perilipin, adipose differentiation-related protein, tail

174 The mammalian proteins Perilipin, ADRP, and TIP47 share extensive amino acid se

175 However, while Perilipin and ADRP localize exclusively to neutral lipid

176 Further, leptin decreased perilipin and fatty acid synthase expression, which play

177 ipid droplet fission, and phosphorylation of perilipin and hormone sensitive lipase - all hallmarks o

178 f attack by hormone-sensitive lipase, and 3) perilipin and hormone-sensitive lipase are continuously

179 Compared to controls, perilipin and leptin mRNA concentrations were lower (P <

180 c agonists triggers rapid phosphorylation of perilipin and translocation of hormone-sensitive lipase

181 into NIH 3T3 fibroblasts lacking endogenous perilipins and HSL but overexpressing acyl-CoA synthetas

182 ession of adipogenic markers (aP2, CD36, and perilipin) and low fatty-acid synthase enzymatic activit

183 CAAT/enhancer-binding protein (C/EBP) alpha, perilipin, and adipocyte-specific fatty acid-binding pro

184 FoxC2 inhibits the expression of C/EBPalpha, perilipin, and adiponectin even in the presence of poten

185 cell, most notably C/EBPalpha, adiponectin, perilipin, and the adipose-specific fatty acid-binding p

186 the adipocyte-specific lipid droplet protein perilipin, and the dead/dying adipocytes are surrounded

187 with mRNA levels of lipid droplet proteins, perilipin, and TIP47 in human subcutaneous adipose tissu

188 tissue and testes, tissues that also express perilipins, and at lower levels in liver, skin, kidney,

189 Both HSL and perilipin are substrates for polyphosphorylation by prot

190 The perilipins are the most abundant proteins coating the su

191 ipin in adipose lipid metabolism and suggest perilipin as a potential target for attacking problems a

192 So far, the use of perilipins as markers for differential diagnosis of soft

193 ide evidence that the protein composition of perilipins at the LD surface regulates lipolytic activit

194 indings, but also showed co-precipitation of perilipin B and CGI-58.

195 In contrast, perilipin B exerts only minimal protection against lipol

196 o 429, but not to lipid droplets coated with perilipin B or mutated perilipin A lacking this sequence

197 e distinct from those droplets surrounded by perilipin; but by 240 min, most perilipin-coated droplet

198 in ligand-induced complex formation between perilipin, caveolin-1, and the catalytic subunit of PKA

199 Like perilipin, Cidea and the related lipid droplet protein C

200 urrounded by perilipin; but by 240 min, most perilipin-coated droplets had some S3-12 on the surface

201 synthesized triacylglycerol is delivered to perilipin-coated lipid droplets is poorly understood.

202 st of their energy as triacylglycerol in the perilipin-coated lipid droplets of adipocytes.

203 Perilipin coats the lipid droplets of adipocytes and is

204 umans, expression of Cidea, Cidec/FSP27, and perilipin correlates positively with insulin sensitivity

205 ases to substrate accessibility, mediated by perilipin-dependent protein sequestration and recruitmen

206 xpression was decreased and insulin-mediated perilipin dephosphorylation was increased in Ad-GcR(-/-)

207 Perilipins drive triacylglycerol storage in adipocytes b

208 Thus, perilipin expression and phosphorylation state are criti

209 man hepatoma cells induced LD deposition and perilipin expression, suggesting a cell autonomous effec

210 that the core functions of Pet10p and other perilipins extend beyond protection from lipases and inc

211 ted amino- or carboxyl-terminal mutations of perilipin failed to serve a dominant negative function i

212 The perilipin family is of ancient origin and has expanded t

213 Members of the perilipin family of lipid droplet scaffold proteins are

214 rator-activated receptor gamma, adiponectin, perilipin, fatty acid synthase, and lipoprotein lipase.

215 These ML adipocytes expressed adiponectin, perilipin, fatty acid-binding protein (FABP), leptin, C/

216 treatment caused an electrophoretic shift of perilipin from 65 to 67 kDa, consistent with perilipin h

217 of the cells was quantified as a measure of perilipin function and was compared with that of cells e

218 orms of the two major splice variants of the perilipin gene, perilipins A and B, in Chinese hamster o

219 The Perilipin homologue LSD2 has been identified as a regula

220 perilipin from 65 to 67 kDa, consistent with perilipin hyperphosphorylation by activated cAMP-depende

221 t with the hypothesis that TNF-alpha induces perilipin hyperphosphorylation by activating PKA, TNF-al

222 The data reveal a major role for perilipin in adipose lipid metabolism and suggest perili

223 To study the role of perilipin in vivo, we have created a perilipin knockout

224 s study was to investigate the expression of perilipins in 478 human soft tissue tumors and 60 respec

225 lipolysis in adipocytes suggests a role for perilipins in this process.

226 ted disruption of the lipid droplet protein, perilipin, in mice leads to constitutional lipolysis ass

227 tion facilitates (either direct or indirect) perilipin interaction with LD-associated HSL.

228 Perilipin is a member of the evolutionarily related fami

229 imulated lipolytic activity, indicating that perilipin is required for maximal lipolytic activity.

230 hosphorylatable perilipin A, confirming that perilipin is required to elicit the HSL translocation re

231 otein kinase A (PKA), and phosphorylation of perilipin is required to induce HSL to translocate from

232 jor control over HSL-mediated lipolysis when perilipin is the main lipid droplet protein.

233 nerated from murine embryonic fibroblasts of perilipin knock-out mice.

234 role of perilipin in vivo, we have created a perilipin knockout mouse.

235 pported by adenoviral expression of a mutant perilipin lacking all six PKA sites (Peri Adelta1-6).

236 ipid droplet (LD) numbers and alterations in perilipin levels, supporting a role for spartin in LD ma

237 termined for hormone sensitive lipase (HSL), perilipin (lipid droplet-associated protein), and two ad

238 Perilipins localize to lipid droplet surfaces and displa

239 Similar to its mammalian counterpart Perilipin, LSD2 is responsible for regulating lipid home

240 Universally, the perilipins modulate cellular lipid storage.

241 Perilipin null (peri(-/-)) and wild-type (peri(+/+)) mic

242 When fed a high-fat diet, the perilipin null animals are resistant to diet-induced obe

243 ineered from murine embryonic fibroblasts of perilipin null mice (Peri-/- MEF), we demonstrate by cel

244 Isolated adipocytes of perilipin null mice exhibit elevated basal lipolysis bec

245 rmed in differentiated brown adipocytes from perilipin null mice expressing an adipose-specific Peri

246 se to the constitutive lipolysis, we studied perilipin-null (plin(-/-)) mice in terms of their fatty

247 Adipocytes from perilipin-null animals have an elevated basal rate of li

248 ocytes derived from embryonic fibroblasts of perilipin-null mice.

249 ith lipid droplet surfaces at a low level in perilipin nulls, but that stimulated translocation from

250 interactions, as shown by the persistence of perilipins on lipid droplets after centrifugation throug

251 tances, a set of structural proteins such as perilipin or adipose differentiation-related protein, en

252 To assess whether perilipins participate directly in PKA-mediated lipolysi

253 ngly regulated by associated proteins of the perilipin/PAT (perilipin, adipophilin, and tail-interact

254 Adipophilin (ADPH/Plin2), a member of the perilipin/PAT family of lipid droplet-associated protein

255 Perilipin (Peri) A is a lipid droplet-associated phospho

256 Perilipin (Peri) A is a phosphoprotein located at the su

257 This suggests that PKA-dependent perilipin phosphorylation facilitates (either direct or

258 It is believed that perilipin phosphorylation is essential for the transloca

259 defects in white fat were caused by impaired perilipin phosphorylation, and the reduced triglyceride

260 nd of the hormone-sensitive lipase (HSL) and perilipin phosphorylation.

261 HSL at the LD surface requires PKA-dependent perilipin phosphorylation.

262 induced HSL translocation was independent of perilipin phosphorylation.

263 etabolites, and (2) potential changes in the perilipin (PLIN) content of the lipid droplets storing i

264 ic cells are coated with at least one of the perilipin (Plin) family of proteins.

265 BFA treatment also increased perilipin (PLIN) family protein PLIN3 but reduced PLIN2

266 whether the presence of polymorphisms at the perilipin (PLIN) locus (PLIN1, 6209T-->C; PLIN4, 11482G-

267 Several perilipin (PLIN) polymorphic sites have been studied for

268 Increased expression of perilipin (PLIN) proteins and colocalisation to LDs has

269 Perilipin (PLIN) proteins constitute an ancient family i

270 T: Because the lipid droplet (LD)-associated perilipin (PLIN) proteins promote intramuscular triglyce

271 EY POINTS: The lipid droplet (LD)-associated perilipin (PLIN) proteins promote intramuscular triglyce

272 fat cells, and there is strong evidence that perilipin (Plin), a lipid droplet scaffold, and adipose

273 hagosome or autophagolysosome formation, and perilipin (PLIN), a lipid droplet-associated protein, su

274 ported that the inactivation of the gene for perilipin (plin), an adipocyte lipid droplet surface pro

275 Perilipins (PLINs) play a key role in energy storage by

276 orescently tagged proteins indicate that: 1) perilipin preferentially targets a special class of peri

277 ter assay and induced lipid accumulation and perilipin protein expression in BMS2 cells.

278 s are coated with one or more members of the perilipin protein family, which serve important function

279 ocytes of OVX-E2 mice contained >3-fold more perilipin protein than adipocytes of pairfed control (OV

280 let translocation or ABHD5 interactions with perilipin proteins and ABHD5 ligands, demonstrating that

281 We investigated mechanisms by which three perilipin proteins control lipolysis by adipocyte trigly

282 Analysis of chimeric and mutant perilipin proteins demonstrated that amino acids 200-463

283 redefine and expand our understanding of how perilipin regulates HSL-mediated lipolysis in adipocytes

284 In mammals, perilipin regulates lipid droplet homeostasis but no suc

285 eins identified in both preparations include perilipin, S3-12, vimentin, and TIP47; in contrast, adip

286 d lipolysis and the electrophoretic shift of perilipin, suggesting a role for PKA in TNF-alpha-induce

287 raction between hormone-sensitive lipase and perilipin, the protein that coats the adipocyte lipid dr

288 Perilipins, the major structural proteins coating the su

289 Perilipins, the most abundant proteins on these lipid dr

290 ion with TNF-alpha and MEK inhibitors caused perilipin to migrate as a single 65-kDa band.

291 review provides a summary that connects the perilipins to both cellular and whole-body homeostasis.

292 lated approximately 4.5-fold less lipid than perilipin-transfected wild-type cells.

293 in fibroblasts stably expressing the mutated perilipin upon incubation with forskolin and IBMX.

294 -L1 cells stably expressing mutated forms of perilipin using microscopy.

295 in-1 null adipocytes, the phosphorylation of perilipin was dramatically reduced, indicating that cave

296 tion of lipolysis associated with absence of perilipin, WAT activated pathways to rid itself of the p

297 kinase 1 (ERK1) and ERK2 and to downregulate perilipin (which has been implicated in the lipolytic ef

298 sitive lipase (HSL), a cytosolic enzyme, and perilipin, which coats the lipid droplet surface in adip

299 ontains a PAT domain, a defining property of perilipins, which was not previously known to exist in y

300 coated with perilipin A or mutated forms of perilipin with an intact C-terminal sequence from amino