ライフサイエンスコーパス: phage T7

1 at of a heterologous RNA polymerase (that of phage T7).

2 igh-fidelity replicative DNA polymerase from phage T7.

3 evels not toxic to Escherichia coli inhibits phage T7.

4 titatively study the population expansion of phage T7.

5 T7 gene 2.5 protein to support the growth of phage T7.

6 gene 32 protein cannot support the growth of phage T7.

7 n from a foreign promoter (PT7) derived from phage T7.

8 estions using Escherichia coli and its lytic phage T7.

9 bunit RNA polymerases (RNAPs) are present in phage T7 and in mitochondria of all eukaryotes.

10 bacteriophage T7 confers sensitivity of both phage T7 and its host Escherichia coli to dideoxythymidi

11 om model organisms such as Escherichia coli, phage T7 and phage T4 has demonstrated the essential nat

12 lity maps for all or most genes in the model phages T7 and T4, as well as the non-model phage Bas63.

13 Like the well characterized primosomes of phages T7 and T4, this trio of proteins coordinates pare

14 sing Escherichia coli biofilms and the lytic phage T7 as models, we discovered that an amyloid fibre

15 This finding explains the sensitivity of phage T7 but not E. coli to exogenous ddT.

16 The 63-kDa gene 4 DNA primase of phage T7 catalyzes the synthesis of oligoribonucleotides

17 inal primase domain of the gene 4 protein of phage T7 comprises a zinc-binding domain that recognizes

18 p new vaccine candidates and refactoring the phage T7 DNA genome are discussed as examples.

19 Phage T7 DNA ligase seals nicked DNA substrates and is a

20 of the same phage DNA by a modified form of phage T7 DNA polymerase (Sequenase) was physically block

21 mer-template junction within the replicative phage T7 DNA polymerase containing an incoming dATP, the

22 with the T4 DNA polymerase holoenzyme or the phage T7 DNA polymerase-thioredoxin complex, both of whi

23 Here, we reconstitute the phage T7 DNA-ejectosome components gp14, gp15, and gp16

24 ional structure of Escherichia coli RecA and phage T7 DnaB (gp4) reveals that the area of sequence co

25 on rates and then measured its production of phage T7 during a single cycle of infection.

26 We created in silico 90,000 mutants of phage T7, each carrying from 1 to 30 mutations, and eval

27 Here, we show our engineered phage T7, enabling the evolution of a theophylline ribos

28 l structure of the junction-resolving enzyme phage T7 endonuclease I in complex with a synthetic four

29 d rRNA operon promoter fused with the E.coli phage T7 gene 10 (T7g10) 5'-untranslated region (5'-UTR)

30 lisome is the ring-shaped helicase TWINKLE-a phage T7-gene 4-like protein expressed in the nucleus an

31 troviruses and from the restructuring of the phage T7 genome.

32 Phage T7 helicase unwinds double-stranded DNA (dsDNA) by

33 Endonuclease I of phage T7 is a member of the nuclease superfamily of prot

34 The 63-kDa gene 4 protein of phage T7 is also a DNA primase in that it catalyzes the

35 e proteins, produced by the Escherichia coli phage T7, is gene product (Gp) 0.4.

36 ed RNA polymerase (vRNAP) is a member of the phage T7-like single-subunit RNA polymerase (RNAP) famil

37 terminase recombinants under the control of phage T7 promoter and overexpressed the packaging/termin

38 When orfX was expressed from a phage T7 promoter in E. coli, a protein with an apparent

39 IRP4 were expressed under the control of the phage T7 promoter in E. coli, polypeptide products of th

40 ence changes in the initiation region of the phage T7 promoter on promoter function.

41 ere to coincide with a bacterial promoter, a phage T7 promoter, a site for gyrase and intrinsically b

42 ructed in a plasmid containing a regulatable phage T7 promoter.

43 YMt and phage T7 promoters differ greatly in sequence and length

44 asymmetric [4 nt/5 nt] internal loop of the phage T7 R1.1 substrate reveals that cleavage reactivity

45 etics to study responses of the heterologous phage T7 replisome to the Tus-Ter complex.

46 We have characterized the roles of the phage T7 RNA polymerase (RNAP) thumb subdomain and the R

47 NA synthesis using in vitro transcription by phage T7 RNA polymerase allows preparation of milligram

48 Phage T7 RNA polymerase contains within its single polyp

49 Furthermore, NEP and the phage T7 RNA polymerase exhibit similar sensitivity to i

50 show here that the pET system, in which the phage T7 RNA polymerase gene is expressed via lac operon

51 The structures of phage T7 RNA polymerase in an elongation phase substrate

52 Phage T7 RNA polymerase is the only DNA-dependent RNA po

53 Transcription driven by phage T7 RNA polymerase was not regulated by cobalamins,

54 Results are obtained using phage T7 RNA polymerase with reconstituted nucleosomes p

55 f tRNA(Gly) to glyQS leader RNA generated by phage T7 RNA polymerase.

56 Yeast mitochondrial (YMt) and phage T7 RNA polymerases (RNAPs) are two divergent repre

57 h polymerases as distinct as E. coli DNAP I, phage T7 RNAP, and E. coli RNAP.

58 eled on the structure of the closely related phage T7 RNAP, appear to lie on one surface of the prote

59 In contrast to phage T7-RNAP, N4 RNAPII displays no activity on double-

60 ated anomalously on SDS-PAGE, similar to the phage T7 scaffolding protein.

61 The phage T7 single-stranded DNA-binding protein gp2.5 speci

62 amino acids of the gene 10 leader peptide of phage T7 (T7.Tag) and the putative sigB gene of S. aureu

63 nded a computer simulation for the growth of phage T7 to account for the physiology of its host.

64 esulting from the promoter-up mutation allow phage T7 to avoid exclusion by the F plasmid, presumably

65 orylase greatly increases the sensitivity of phage T7 to ddT.

66 In this study, we used the phage T7 transcript of the tRNA as a substrate.

67 amber suppressor tRNA) or a test tube assay (phage T7 tRNA substrate and purified enzyme).

68 IN does not strongly protect against another phage, T7, which incompletely blocks host transcription.