ライフサイエンスコーパス: phagemid

1 capsulation of any therapeutic gene encoding phagemid.

2 y the phage helper and are separate from the phagemid.

3 cassettes) necessary for replication of the phagemid and expression of the marker gene in the target

4 Escherichia coli was transformed with the phagemids and infected with VCSM13 helper phage, and the

5 chain Fv (scFv) gene repertoire from a naive phagemid antibody library into a true phage vector to cr

6 with type 1 diabetes are contained within a phagemid artificial chromosome clone of 100 kb, suggesti

7 cells eliminate the use of helper phage from phagemid-based selection protocols; reducing the amount

8 ss-reacting cI variants, we design a new M13 phagemid-based system for the directed evolution of biom

9 F), similar to the peptide identified in the phagemid, bound fibronectin in a concentration-dependent

10 One of the isolated phagemid clones showed significant homology to the gene

11 d that requires generation of noninfectious, phagemid-containing virion-like particles.

12 ction and optimization through combinatorial phagemid display, protein crystallography, and further m

13 e proteins required for encapsulation of the phagemid DNA and cell targeting are provided by the phag

14 c strain was generated to allow packaging of phagemid DNA into P1 phage having either S or S' tail fi

15 P1(S') could transduce phagemid DNA into Shigella flexneri 2a 2457O, Shigella f

16 erapeutic gene can be easily exchanged for a phagemid expressing a different therapeutic gene.

17 glioma cells, differs from others in that a phagemid expressing a model marker or particular therape

18 of two pairs of single stranded circles from phagemids, followed by their sequential annealing with r

19 We developed a phagemid format wherein antibody heavy- and light-chain

20 rom a landscape phage display library, and a phagemid harboring a marker gene and all regulatory elem

21 dues in single-stranded DNA generated from a phagemid, in which we sequenced an insert representing t

22 Therefore, the resultant Phagemid Infective Particles (PIPs) are able to bind and

23 mproved the delivery of a cas9 antimicrobial phagemid into both S. flexneri 2457O and M90T.

24 ith a large gap, or single-stranded circular phagemid is sufficient to induce a p53-dependent arrest.

25 In this study, a VHH phagemid library generated from a llama that was multipl

26 Clones from a phagemid library, expressed as gene-3 fusion proteins on

27 The procedure entails generation of an M13-phagemid library, production of an ORFeome (totality of

28 -binding scFv were also higher than from the phagemid library.

29 m the phage library were lower than from the phagemid library.

30 ibrary, can be modulated between monovalent (phagemid-like) and multivalent display (phage-like) with

31 troduced into a RNase T+ cell on a multicopy phagemid, most likely as a consequence of inactive heter

32 nterica Typhimurium strain chi3987 harboring phagemid NgoPhi6 fm.

33 The poor correlation between phagemid particle fluorescence and recognition of GFP by

34 Correct folding and display were assessed by phagemid particle fluorescence, and with anti-GFP antibo

35 This results in genetically pure phagemid particle preparations.

36 ress phage packaging proteins which assemble phagemid particles as efficiently as helper phage, but w

37 ted to alanine and displayed monovalently on phagemid particles as gene III fusion proteins.

38 iversity, and effectively using the packaged phagemid particles as means to transfer genetic informat

39 pression by epidermal growth factor-targeted phagemid particles increased with heat shock, UV irradia

40 ssential for the replication and assembly of phagemid particles, during library production and biopan

41 rotein fusions, on the surface of a phage or phagemid particles.

42 om a large repertoire of antibody-expressing phagemid particles.

43 ernal restriction sites, and cloned into the phagemid pCOMB3H for expression as fusion constructs wit

44 ages: (i) preparation of a combinatorial M13 phagemid (PM) library expressing variants of the gene of

45 ain genes were amplified and cloned into the phagemid pSD3 for generation of a recombinant antibody l

46 As an example we present a phagemid retroviral shuttle vector that can be used to a

47 Southern analysis of the resulting phagemids revealed that a 0.5-kb EcoRI fragment hybridiz

48 fied oligomers inserted into single-stranded phagemid shuttle vectors were used to transform E. coli

49 studied in Escherichia coli using an in vivo phagemid system as a model for continuous leading strand

50 ineered Escherichia coli 'helper' strain and phagemid system that simplifies long ssDNA generation to

51 xpressed in mammalian, Escherichia coli, and phagemid systems.

52 We prepared a single-stranded phagemid template containing a dT41 sequence to test the

53 In the resulting phagemid, the loxP sequence also encodes a polypeptide l

54 he sybody sequences are transferred from the phagemid to different expression vectors without the nee

55 6)-fold and delivered these combinations via phagemids to increase the killing of highly drug-resista

56 paranemic cross-over DNA) is inserted into a phagemid, transformed into XL1-Blue cells and amplified

57 the Fabs are located in the backbone of the phagemid vector and the library insert comprises only th

58 ated, transcribed to cDNA, and cloned into a phagemid vector for phage display library construction.

59 This phagemid vector system provides a way to recombine VH an

60 er process, we have constructed a "drop-out" phagemid vector that can be rapidly converted to an expr

61 We cloned these genes into a phagemid vector, expressed these clones as single-chain

62 lasmid vector, pCW93, and a pACYC184-derived phagemid vector, pCW99, respectively.

63 n protein consisting of adapter GR1 from the phagemid vector, while the recombinant phage coat protei

64 nd lambda isotype by cloning into the pHENIX phagemid vector.

65 ras1 gene were inserted into single-stranded phagemid vectors and transfected into COS-7 cells.

66 ted naive libraries have been constructed in phagemid vectors as fusions to pIII, yielding primarily

67 roteins pIII, pVII, and pVIII using the same phagemid vectors combined with different helper phage ve

68 cleotides were inserted into single-stranded phagemid vectors followed by transfection into simian ki

69 oligomers were inserted into single-stranded phagemid vectors.

70 sion were inserted into single-stranded (ss) phagemid vectors.