ライフサイエンスコーパス: phagophore

1 and so directing lipid supply to the nascent phagophore.

2 or VPS4 inhibition accumulates VPS37A on the phagophore.

3 rowing autophagosomal membrane, known as the phagophore.

4 ormation but not for Optn recruitment to the phagophore.

5 barrier in the transition to the cup-shaped phagophore.

6 the ULK1 autophagy initiation complex to the phagophore.

7 equires activation of the ULK complex at the phagophore.

8 -induced centrosomal GABARAP delivery to the phagophore.

9 he mobilization of membranes to generate the phagophore.

10 folds for assembly of the ULK complex at the phagophore.

11 which in turn initiates the formation of the phagophore.

12 organization of Atg9 vesicles into the early phagophore.

13 sealing of single-membrane cisternae called phagophores.

14 ne (PE) in double-membrane structures called phagophores.

15 sphate and directs atg8ylation of autophagic phagophores.

16 s, which form from precursors referred to as phagophores.

17 e of membrane shape and curvature in growing phagophores.

18 ransporter densities connected to mitophagic phagophores.

19 membrane is required for its recruitment to phagophores.

20 f the Atg12-5-16L1 complex to Wipi2-positive phagophores.

21 ted Mtb and targets bacteria to LC3-positive phagophores.

22 n of these vesicles and their fusion to form phagophores.

23 CP1-positive autophagosome precursors called phagophores.

24 Forming the autophagosome from the phagophore, a cup-shaped double-membrane vesicle, is a c

25 mmalian studies have inferred that mammalian phagophores also have single openings.

26 is regulated for efficient formation of the phagophore, an isolation membrane that sequesters the pe

27 a very early event in the generation of the phagophore and in the process of macroautophagy.

28 ar organization of contact sites between the phagophore and organelles, such as the vacuole and the e

29 ositive structures interact dynamically with phagophores and autophagosomes without being incorporate

30 It is recruited at early stages to phagophores and is required for their maturation into au

31 lead to impairment in isolation membrane (or phagophore) and autophagosome formation, maturation, or

32 xpansion of the autophagosome precursor, the phagophore, and give the first real-time, observation-ba

33 of the initial sequestering compartment, the phagophore, and is proposed to play a key role in membra

34 The finger-shaped phagophore apertures remained open when ESCRT function w

35 ore, our observations support the model that phagophores are initiated by the accumulation of autopha

36 of autophagy, and juxtaposition of cargo and phagophores are integrated in higher eukaryotes.

37 Whether phagophores are recruited from a constitutive pool or ar

38 tudy provides insights into the formation of phagophores around one of the largest selective cargoes.

39 he outer membrane of Salmonella, or existing phagophores as templates.

40 RAPPIII complex directs this Ypt1 GEF to the phagophore assembly site (PAS) that is involved in autop

41 fects the recruitment of mitochondria to the phagophore assembly site (PAS), a critical step in the p

42 Most of them reside at the phagophore assembly site (PAS), although the function of

43 tg17-Atg31-Atg29 complex translocates to the phagophore assembly site (PAS), where an autophagosome f

44 utophagy begins with the organization of the phagophore assembly site (PAS), where most of the AuToph

45 through what mechanism Atg9 functions at the phagophore assembly site (PAS).

46 COG subunits localized to the phagophore assembly site and interacted with Atg (autoph

47 that TRAPPIII binds to COPII vesicles at the phagophore assembly site and that COPII vesicles may pro

48 s that are dependent on PtdIns(3)P for their phagophore assembly site association.

49 conjugation systems, Atg8 and Atg16, to the phagophore assembly site is affected.

50 of the essential autophagy machinery to the phagophore assembly site is impaired.

51 the Rab GTPase Ypt1 that is recruited to the phagophore assembly site when macroautophagy is induced.

52 Additionally, the pexophagy-specific phagophore assembly site, organized by the dual interact

53 tant, Atg8 is inefficiently recruited to the phagophore assembly site, which is involved in autophago

54 ed the initial protein assembly steps at the phagophore assembly site.

55 ing to phagophore formation initiated at the phagophore assembly site.

56 ely downstream of the Atg1 kinase complex at phagophore assembly sites.

57 R-mitochondria contact sites are involved in phagophore assembly.

58 ly unknown; we confirm roles for Gyp1 at the phagophore-assembly site, Atg24 in cargo engulfment, Atg

59 the autophagy pathway via interactions with phagophore-associated ATG8-like proteins.

60 ere we show that Bcl-2 directly binds to the phagophore-associated protein GABARAP.

61 We show that host cells generate multiple phagophores at the site of damaged Salmonella-containing

62 These phagophores attach to donor membranes, expand via ATG2-m

63 lated protein 7 (Atg7), proteins involved in phagophore-autophagosome formation, in ALD.

64 rive the first membrane recruitment event of phagophore biogenesis in xenophagy and mitophagy.

65 xotrophic Salmonella to study the process of phagophore biogenesis using in situ correlative cryo-ET.

66 Cargo initiates phagophore biogenesis, which entails the conjugation of

67 ATG12-5-16L1 is recruited to the phagophore by a subset of the phosphatidylinositol 3-pho

68 During autophagy, phagophores capture portions of cytoplasm and form doubl

69 unique set of ESCRT machinery components for phagophore closure in mammalian cells.

70 The process of phagophore closure requires the endosomal sorting comple

71 C3-lipid conjugation but also for subsequent phagophore closure, where TfR-dependent PI(3)P recruits

72 I subunit VPS37A as a critical component for phagophore closure.

73 flat double-membraned vesicles (sequestosome:phagophore complexes), sequestosomes within double-membr

74 A vesicle, possibly a precursor of the phagophore, contains translocated m-IL-1beta and later t

75 Rop1 is needed for the formation of phagophores, cup-like structures consisting of two close

76 nwrapped by the autophagosome precursor, the phagophore, due to a lack of mitochondrial fission.

77 recruitment of PI3P-binding proteins to the phagophore during autophagy induction, suggesting that a

78 conjugated by LC3 family members from which phagophores emanate.

79 diated transfer of lipids from the ER to the phagophore enables phagophore expansion.

80 ear membrane fission occurs independently of phagophore engagement and instead relies surprisingly on

81 ocess whereby newly formed membranes, termed phagophores, engulf parts of the cytoplasm leading to th

82 vesicular phospholipid transfer (PLT) across phagophore-ER contacts (PERCS).

83 Currently, the phagophore-ER tether Atg2 is the only PLT protein known

84 ve and nonselective autophagy at the step of phagophore expansion at the PAS.

85 Atg2 is the only PLT protein known to drive phagophore expansion in vivo.

86 culum (ER), is suggested to be essential for phagophore expansion, but the underlying mechanism remai

87 lipids from the ER to the phagophore enables phagophore expansion.

88 key for its efficient catalysis and promotes phagophore expansion.

89 How phagophores form and expand on such a large cargo remain

90 well appreciated that autophagy begins with phagophore formation and expansion through lipid acquisi

91 Phagophore formation in situ would require recruitment o

92 de novo by membrane fusion events leading to phagophore formation initiated at the phagophore assembl

93 al damage but is not involved in the initial phagophore formation on terminally damaged lysosomes.

94 Thus, TRPML1 activation of phagophore formation requires the calcium-dependent kina

95 ion facilitates membrane flow to the PAS for phagophore formation.

96 remodeling thus emerges as a viable route to phagophore formation.

97 x, activation of CAMKK2, AMPK, and ULK1, and phagophore formation.

98 d specifically for pexophagy at the stage of phagophore formation.

99 Phagophores grow by lipid influx from the endoplasmic re

100 ance mechanism in which the double-membraned phagophore grows and engulfs cytosolic material.

101 xpand the initial sequestering membrane, the phagophore; however, essentially nothing is known about

102 sential PI3P-binding proteins to the nascent phagophore in a TFEB-independent manner.

103 zes to the rim and promotes the expansion of phagophores in parallel with Atg2.

104 mAtg9 is recruited to phagophores independent of early autophagy proteins, suc

105 STX13 was present on LC3-positive phagophores induced by rapamycin and was highly enriched

106 and plays a previously unrecognized role in phagophore initiation from ER exit sites.

107 ates LC3-II production and maturation of the phagophore into the autophagosome, by facilitating the r

108 nction and participates in the maturation of phagophores into closed autophagosomes.

109 phagy, rapid membrane assembly expands small phagophores into large double-membrane autophagosomes.

110 sphatidylethanolamine in the double-membrane phagophore is a key event in the early phase of macroaut

111 The phagophore is largely devoid of integral membrane protei

112 The origin of the cup shape of the phagophore is poorly understood.

113 iated Atg12-5-16L1 complex to Wipi2-positive phagophores is crucial for producing microtubule-associa

114 ase, TMEM41B, and also in close proximity to phagophores, lipid droplets and mitochondria.

115 Phagophore maturation is a key step in the macroautophag

116 w that 1 novel hit, TMEM41B, is required for phagophore maturation.

117 s, thus contributing to the expansion of the phagophore membrane in the early stages of autophagy.

118 How the highly curved phagophore membrane is stabilized during autophagy initi

119 -C1) and conjugation of ATG8/LC3 proteins to phagophore membranes by the ATG12-ATG5-ATG16L1 (E3) comp

120 ceptor (TfR) links LC3 family conjugation to phagophore membranes, an early step in autophagosome bio

121 cargo receptors that juxtapose bacteria and phagophore membranes.

122 In yeast, the phagophore nucleates from a cluster of 20-30 nm diameter

123 How phagophore nucleation and expansion are coordinated with

124 ts suggest a positive feedback loop coupling phagophore nucleation and expansion to lipid synthesis.

125 g31-Atg29 subcomplex of Atg1 is critical for phagophore nucleation at the PAS.

126 The intermediate membrane, termed the phagophore or isolation membrane, is a cup-like structur

127 o play a key role in membrane transport; the phagophore presumably expands by vesicular addition to f

128 Notably, loss of VPS37A abrogates the phagophore recruitment of the ESCRT-I subunit VPS28 and

129 the ATG12-ATG5-ATG16L1 complex to the early phagophore rim could stabilize membrane curvature and fa

130 Growth along the phagophore rim marks the progress of both organelle expa

131 ponent Atg2 that is thought to reside at the phagophore rim.

132 ntermembrane distance that is dilated at the phagophore rim.

133 Phagophore rims establish different forms of contact wit

134 d ATG8s anchored to the outer surface of the phagophore serve as scaffolds for binding of other core

135 st three vesicles need to fuse to induce the phagophore shape, consistent with experimental observati

136 Compatible phagophore structures were observed with whole-mount and

137 brane, and in their homotypic fusion to form phagophore structures.

138 me maturation by AMPK-mediated inhibition of phagophore tethering to donor membrane.

139 rmation of a cup-shaped structure called the phagophore that matures into the autophagosome.

140 d by the formation of an isolation membrane (phagophore) that engulfs cytoplasmic constituents, leadi

141 t and assemble the conjugation system at the phagophore, the nascent autophagosome.

142 rs and receptors that become tethered to the phagophore through interactions with lipidated ATG8/LC3

143 VPS37A localizes on the phagophore through the N-terminal putative ubiquitin E2

144 fusion of the edges of the double-membraned phagophores to form autophagosomes.

145 phatidylinositol 3-phosphate (PI3P)-enriched phagophores to the endoplasmic reticulum (ER), is sugges

146 presumably targets the enzyme to the tip of phagophore, to the C-terminally located catalytic core f

147 phagy and for the forces shaping and driving phagophores toward closure without a templating cargo.

148 However, the number of Wipi2-positive phagophores was not decreased in Optn-deficient cells.

149 Phagophores were visualized, with bridge-like lipid tran

150 proceeds by the growth of a double-membraned phagophore, which engulfs cytosol and other substrates.

151 t is the expansion of the precursor membrane phagophore, which requires the acquisition of lipids to

152 s are formed by the elongation and fusion of phagophores, which can be derived from preautophagosomal

153 ange from disk-shaped to expanded cup-shaped phagophores, which have a thin intermembrane lumen with

154 d transport is then used to feed the growing phagophore with pre-selected cargoes and debris derived

155 ins that evolved into unexpected finger-like phagophores with multiple openings before becoming more

156 yeast autophagosomes evolve from cup-shaped phagophores with only one closure point, and mammalian s