ライフサイエンスコーパス: phosphoramidite

1 l-telluride-thymidine derivatives and the Te-phosphoramidite.

2 two efficient routes from [3-15N]-thymidine phosphoramidite.

3 e-U-RNAs as well as the 2-Se-uridine ((Se)U) phosphoramidite.

4 synthetically from either D- or L-nucleoside phosphoramidites.

5 n moving from Taddol-based to biphenol-based phosphoramidites.

6 s comparable to those of deoxyribonucleoside phosphoramidites.

7 nthesis are comparable to those for standard phosphoramidites.

8 sis of RNA oligonucleotides using nucleoside phosphoramidites.

9 red the synthesis of the appropriate reverse phosphoramidites.

10 hieved with 2-cyanoethyl deoxyribonucleoside phosphoramidites.

11 y.dA were introduced by using the respective phosphoramidites.

12 commercial 2-cyanoethyl deoxyribonucleoside phosphoramidites.

13 )-pteridone (DMAP), have been synthesized as phosphoramidites.

14 the cross-link using protected nucleoside 5'-phosphoramidites.

15 ing of the nucleophile incorporated in these phosphoramidites.

16 the cross-link using protected nucleoside 3'-phosphoramidites.

17 by TBS for the convenience of conversion to phosphoramidites.

18 nctionalized triazole-linked 2'-deoxyuridine phosphoramidites.

19 ipulations to provide the desired pyrimidine phosphoramidites.

20 Two novel DNA base surrogate phosphoramidites 1 and 2, based upon relatively electron

21 The synthesis of the target phosphoramidites 1-4 is initiated from pentafuranose 5,

22 The preparation of phosphoramidites 10a-d is straightforward, and the incor

23 Phosphoramidites 15a and 15b were synthesized in 11 step

24 When employing phosphoramidites 1a-d in the solid-phase synthesis of ol

25 -1-propyl group into the deoxyribonucleoside phosphoramidites 1a-d is achieved using inexpensive raw

26 ng group (CpG ODN fma1555) was prepared from phosphoramidites 1a-d using solid-phase techniques.

27 were prepared in high yield and purity using phosphoramidites 2 and 3, respectively.

28 Phosphoramidite 21 was prepared from intermediate 11b in

29 Phosphoramidites 23 and 25 were prepared from 2,4-diamin

30 d in the preparation of four deoxynucleoside phosphoramidites 28 and 65-67, plus the 2'-O-(2-methoxye

31 Phosphoramidites 36 and 37 were prepared from cytidine i

32 Phosphoramidites 38 and 39 were prepared from uridine in

33 The total time for synthesis of the phosphoramidite 4 is about 75 h, whereas the total time

34 The most effective ligands identified are phosphoramidite 4, derived from BINOL and N-methylanilin

35 5 mol % Rh(nbd)2BF4, 1.1 mol % BINOL-derived phosphoramidite 4, THF, 40 degrees C, 2 h) affording an

36 Phosphoramidites 4 and 8 were prepared and used in solid

37 Phosphoramidites 40 and 41 were synthesized from uridine

38 synthesis of 3-methylpseudouridine (m(3)Psi) phosphoramidite, 5'-O-[benzhydryloxybis(trimethylsilylox

39 ntermediate, silylated nucleoside amino acid phosphoramidite 6, prepared from a series of reactions s

40 us the 2'-O-(2-methoxyethyl)-5-methyluridine phosphoramidite 68.

41 l were incorporated into deoxyribonucleoside phosphoramidites 7a-d and 9, which were found as efficie

42 corporation of activated deoxyribonucleoside phosphoramidite 8b into the oligonucleotide chain during

43 eport we describe a new ferrocene-containing phosphoramidite 9 that provides a range of detectable re

44 rough subsequent phosphorylation effected by phosphoramidite 9.

45 for 2'-hydroxyl protection of ribonucleoside phosphoramidites 9a-d has been implemented (Schemes 1 an

46 m a mixture of [3-15N]-labeled thymine dimer phosphoramidites after which the cis-syn dimer-containin

47 Use of a bis-2-naphthyl phosphoramidite allowed the successful cycloaddition of

48 The availability of the phosphoramidite allows for reliable and efficient synthe

49 Phosphoramidite analogues of modified cyclotriphosphates

50 ly chiral YanPhos, bearing an N-benzyl BINOL-phosphoramidite and a BINAP-bis(3,5-t-Bu-aryl)phosphine,

51 e, we describe conversion of the ligand to a phosphoramidite and direct incorporation of this moiety

52 a 3-deaza analog of 3MeA (3dMeA) as a stable phosphoramidite and have incorporated the analog into sy

53 nthesized the novel 2'-methylseleno cytidine phosphoramidite and improved the accessibility of the 2'

54 ield of 0.88, and it can be synthesized as a phosphoramidite and incorporated into oligonucleotides t

55 d for the first time the 6-Se-deoxyguanosine phosphoramidite and incorporated it into DNAs via solid-

56 gents through the preparation of a caged DNA phosphoramidite and its site-specific incorporation into

57 synthesizing the novel 4-Se-uridine ((Se)U) phosphoramidite and Se-nucleobase-modified RNAs ((Se)U-R

58 The phosphoramidite and the hydroxy group are reacted in ste

59 osine, which was converted to the nucleoside phosphoramidite and used in the preparation of oligodeox

60 NPdR can be converted to its 5'-DMTr-3'-CE-phosphoramidite and was incorporated into pentacosanucle

61 mon dimethoxytrityl-protected mononucleotide phosphoramidites and a single orthogonally protected tri

62 taining nucleosides are converted into their phosphoramidites and are utilized for the high-yield pre

63 ese nucleosides were converted to their 3'-O-phosphoramidites and controlled-pore glass solid support

64 osides were converted into the corresponding phosphoramidites and incorporated into oligodeoxynucleot

65 esign, commencing with acyclic amine-derived phosphoramidites and leading to cyclic pyrrolidine and a

66 Individual diastereomeric phosphoramidites and mixtures of diastereomeric phosphor

67 od is compatible with commercially available phosphoramidites and other oligonucleotide synthesis rea

68 amethylphosphorous triamide (HMPT) and other phosphoramidites and phosphites have been found to be ef

69 cleosides and their corresponding nucleoside phosphoramidites and solid supports.

70 the synthesis of the cMOE and cEt nucleoside phosphoramidites and the biophysical evaluation of oligo

71 yclohexenyl nucleic acid (F-CeNA) pyrimidine phosphoramidites and the synthesis and biophysical, stru

72 lock, the 2'-SeMe-modified guanosine (G(Se)) phosphoramidite, and report the first incorporation of t

73 novel 2-Se-thymidine ((Se)T) derivative, its phosphoramidite, and the Se-DNAs.

74 ew L-threose-based nucleoside analogues, TNA phosphoramidites, and TNA triphosphates.

75 Also, a benzotriazolyl nucleoside phosphoramidite appears to be a suitable reagent for inc

76 d into oligomeric DNA using the standard DMT-phosphoramidite approach in an automated solid-phase syn

77 The phosphoramidite approach is used to attach these redox a

78 an be further processed to the corresponding phosphoramidite as DNA building blocks that allow incorp

79 By using our own-developed C2-unsymmetric phosphoramidite as supporting ligand, this dipole was ap

80 s with aryl boroxines using a TADDOL-derived phosphoramidite as the ligand provides the corresponding

81 A novel solid-phase phosphoramidite based oligodeoxynucleotide two-step synt

82 Four non-nucleoside JOE phosphoramidites based on 5- and 6-isomers and flexible

83 A Cu(I)-phosphoramidite-based catalytic system that allows asymm

84 nt hydroxyl groups suitable for conventional phosphoramidite-based nucleic acid synthesis.

85 The efficiency of phosphoramidite-based oligonucleotide synthesis on these

86 Nucleoside phosphoramidites bearing a fluorous dimethoxytrityl (FDM

87 -2-cyclohexen-1-one with the Feringa (S,R,R)-phosphoramidite binaphthol ligand, followed by aldol con

88 A corresponding phosphoramidite building block was then prepared and use

89 nd, the nucleosides 1c and 3c as well as the phosphoramidite building blocks 19 and 23 were prepared

90 Phosphoramidite building blocks and oligonucleotides wer

91 l, and pyrenyl)-deoxyguanosine (dG) modified phosphoramidite building blocks and the corresponding da

92 We report the synthesis of new phosphoramidite building blocks and their use for the mo

93 Here we report synthesis of chiral phosphoramidite building blocks based on (R)-4-amino-2,2

94 rans, R and S O(6)-allyl-protected N(2)-dGuo phosphoramidite building blocks derived through cis and

95 f the thiosugar into the four ribonucleoside phosphoramidite building blocks was accomplished with ad

96 Using (6-(13)C)pyrimidine phosphoramidite building blocks, site-specific labels ca

97 Modified phosphoramidites carrying additional thymine or adenine

98 d using a nucleophilic N-heterocycle-flanked phosphoramidite catalyst in combination with the common

99 By combining a tailored chiral iridium phosphoramidite catalyst, which controls regioselectivit

100 onjunction with P1(R,R,R) or P2(S,S,S) of Ir-phosphoramidite catalysts).

101 vely, when bound to chiral Cu-Walphos and Ir-phosphoramidite catalysts.

102 s or cis forms of dG-N2-TAM were prepared by phosphoramidite chemical procedure and used as templates

103 oding property of the N(2)-Et-dG DNA adduct, phosphoramidite chemical synthesis was used to prepare s

104 rates using light-directed photolithographic phosphoramidite chemistry and evaluate the stabilities o

105 ive of glycidaldehyde, was synthesized using phosphoramidite chemistry and site-specifically incorpor

106 The oligomers were prepared using automated phosphoramidite chemistry and terminate with a 5'-phosph

107 hesized from the same twisted monomers using phosphoramidite chemistry folded into homodimer and hete

108 ated with bromophosphates or synthesized via phosphoramidite chemistry in moderate yields (44-77%.) T

109 Despite the ubiquitous use of phosphoramidite chemistry in the synthesis of biophospha

110 ophobic units are usually attached employing phosphoramidite chemistry involving a DNA synthesizer.

111 ort the synthesis of fdG-modified DNAs using phosphoramidite chemistry involving only three steps.

112 se oligonucleotide synthesis (SPOS) based on phosphoramidite chemistry is currently the most widespre

113 into DNA oligonucleotides using solid-phase phosphoramidite chemistry is reported.

114 Traditional phosphoramidite chemistry using 5'-O-DMT protection can

115 incorporated into DNA or tc-DNA by standard phosphoramidite chemistry.

116 S) microchannels through use of conventional phosphoramidite chemistry.

117 cient and facile solid-phase synthesis using phosphoramidite chemistry.

118 ied with oligonucleotides utilizing standard phosphoramidite chemistry.

119 n ink-jet printing method employing standard phosphoramidite chemistry.

120 an automated DNA synthesizer using standard phosphoramidite chemistry.

121 have been prepared on DNA synthesizers using phosphoramidite chemistry.

122 ncoded sequences could be easily built using phosphoramidite chemistry.

123 ed HPLC separation method, the four possible phosphoramidites (cis/trans, R/S) of the B[a]P DE-2 N2-d

124 a deoxy-T derivative with the support-bound phosphoramidite compares well with the nucleoside loadin

125 4-iminocrotonates catalyzed by a rhodium(I)/phosphoramidite complex is described.

126 using an achiral catalyst, and then explore phosphoramidite-complexed rhodium catalysts in the enant

127 imidazole catalyzed by metallacyclic iridium-phosphoramidite complexes, in combination with studies o

128 of X-ray structures of rhodium(cod)chloride/phosphoramidite complexes, we have formulated a mechanis

129 These phosphoramidites contained trityl (A, G, C, and U), dime

130 hemical element, the catalyst derived from a phosphoramidite containing a biphenolate group was studi

131 Fapy.dG containing dinucleotide phosphoramidites containing 5'-thymidine (11a) or 5'-deo

132 ed by a readily available chiral monodentate phosphoramidite-copper complex in the presence of an alk

133 dified nucleosides requires the synthesis of phosphoramidites corresponding to all four canonical bas

134 was then assembled from these synthons using phosphoramidite coupling chemistry.

135 Phosphoramidite coupling steps allowed rapid synthesis o

136 of two successive chemoselective steps: (i) phosphoramidite coupling, and (ii) radical-radical coupl

137 pha-D-cholesterylglucoside at C6 followed by phosphoramidite coupling.

138 A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce fou

139 rmations are promoted by a readily prepared (phosphoramidite)-Cu complex and involve bench-stable gam

140 , 2'-deoxy-2'-C-methylcytidine (12), and the phosphoramidite derivative of 2'-deoxy-2'-C-beta-methylc

141 Here, we report the first synthesis of the phosphoramidite derivative of dinucleotide SP TpT, which

142 thesis of a novel 2'-O-methyl (OMe) riboside phosphoramidite derivative of the G-clamp tricyclic base

143 the sequence phosphitylated to generate the phosphoramidite derivative on the 5'-terminus of the sup

144 dehalogenase-compatible chloroalkane linker phosphoramidite derivative that enables the rapid automa

145 ynthesized, converted into the corresponding phosphoramidite derivatives and introduced into short ol

146 luene deoxyribosides into suitably protected phosphoramidite derivatives and their incorporation into

147 s could be incorporated readily as protected phosphoramidite derivatives into synthetic oligonucleoti

148 the group II intron, we synthesized six new phosphoramidite derivatives of 2'-deoxy-2'-C-alpha-(hydr

149 Synthetic routes to the phosphoramidite derivatives of 2'-deoxy-2'-C-alpha-methy

150 The chemical synthesis of phosphoramidite derivatives of all four 5'-deoxy-5'-thio

151 thods for the synthesis of five C-nucleoside phosphoramidite derivatives that, when used in combinati

152 Suitably protected phosphoramidite derivatives were prepared for incorporat

153 The base-protected phosphoramidite derivatives were prepared, and short oli

154 lpha-mannosyl and alpha-N-acetyl-glucosamine phosphoramidite derivatives with the serine hydroxyl of

155 into oligonucleotides as suitably protected phosphoramidite derivatives.

156 s of 3'-modified DNAs is illustrated using a phosphoramidite derived from tyrosine.

157 way in which the electronic structure of the phosphoramidite dramatically influences reaction rate an

158 ion of two complete series of RNA nucleoside phosphoramidites; each has an appropriately protected am

159 Conversion to the corresponding phosphoramidite enables solid-phase synthesis of [2'-(18

160 single orthogonally protected trinucleotide phosphoramidite (Fmoc-TAG; Fmoc = 9-fluorenylmethoxycarb

161 p through a multistep process: Reaction with phosphoramidite followed by oxidation provided the 5'-mo

162 A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesi

163 2a is amenable to formation of the requisite phosphoramidite for solid-phase oligonucleotide synthesi

164 p route to 5-hydroxymethylcytidine (hm(5)rC) phosphoramidite for solid-phase synthesis of modified RN

165 accessibility of the 2'-methylseleno uridine phosphoramidite for the synthesis of many selenium-deriv

166 The application of several of these phosphoramidites for solid-phase synthesis of oligoribon

167 We describe the synthesis of new phosphoramidites for the incorporation of 7-substituted-

168 lized thymine and 5-methylcytosine amino-LNA phosphoramidites from these key intermediates, respectiv

169 Deoxyribonucleoside phosphoramidites functionalized with the 5'-siloxyl ethe

170 e oligonucleotides, the use of nucleoside 5'-phosphoramidites has not generally been used for the ela

171 otriazol-1-yl)-2'-deoxyinosine 5'-O-DMT 3'-O-phosphoramidite, has been prepared and a preliminary eva

172 fficient as 2-cyanoethyl deoxyribonucleoside phosphoramidites in solid-phase oligonucleotide synthesi

173 any sequence are attainable by synthesizing phosphoramidites in which the hydroxyl groups of the cro

174 is compound has now been synthesized and its phosphoramidite incorporated site-specifically into a de

175 Readily available phosphoramidites incorporating TADDOL-related diols with

176 midine was synthesized and incorporated as a phosphoramidite into oligonucleotide sequences.

177 The yields of coupling these Se-nucleoside phosphoramidites into DNA or RNA oligonucleotides were o

178 ted new photocaged guanosine and uridine RNA phosphoramidites into short RNA duplexes.

179 5'-O-BzH-2'- O -ACE-protected pseudouridine phosphoramidite is reported [BzH, benzhydryloxy-bis(trim

180 thylpseudouridine-3'-(methyl-N,N-diisopropyl)phosphoramidite, is reported.

181 ning Fapy.dG, which does not require reverse phosphoramidites, is described.

182 sized by coupling cytidylyl-(3',5')-cytidine phosphoramidite isotopomers as the common synthetic inte

183 iphosphorus ligands, consisting of phosphine-phosphoramidites L1 and L2 and phosphine-phosphonites L3

184 rdination in a Pd(II) complex of a phosphine-phosphoramidite ligand 1, which showed excellent enantio

185 A chiral phosphoramidite ligand and the precise stoichiometry of

186 hip between the stereochemical elements of a phosphoramidite ligand and the stereoselectivity of irid

187 The original process was conducted with a phosphoramidite ligand containing a resolved 2,2-dihydro

188 ion was the development of a new BINOL-based phosphoramidite ligand containing bulky substitution at

189 phosphates are suitable substrates using new phosphoramidite ligand D.

190 a metallacyclic iridium complex containing a phosphoramidite ligand derived from (R)-1-(2-naphthyl)et

191 The monodentate phosphoramidite ligand exhibits superb reactivity (TONs

192 Manipulation of the phosphoramidite ligand leads to tuning of enantio- and p

193 adium-TMM complex in the presence of a novel phosphoramidite ligand possessing a stereogenic phosphor

194 During catalyst activation, a complex of a phosphoramidite ligand possessing one axial chiral binap

195 An enantioenriched phosphoramidite ligand promotes the copper catalyzed con

196 In the presence of a chiral phosphoramidite ligand, the palladium-catalyzed diborati

197 sence of a chiral monodentate taddol-derived phosphoramidite ligand, these reactions occur with high

198 copper catalyst bearing a bulky monodentate phosphoramidite ligand, which is essential for attaining

199 tained, which is enabled by a TADDOL-derived phosphoramidite ligand.

200 Copper catalyst promotion with both chiral phosphoramidite ligands and a phosphate additive is vita

201 plication of a new class of non-C2-symmetric phosphoramidite ligands derived from pyroglutamic acid f

202 Central to this effort were the novel phosphoramidite ligands developed in our laboratories.

203 Monodentate phosphoramidite ligands have been developed based on ena

204 (PinBH) using simple chiral monophosphite or phosphoramidite ligands in combination with Rh(nbd)2BF4.

205 In this article the utility of phosphoramidite ligands in enantioselective Au(I) cataly

206 ds and selectivities making use of our novel phosphoramidite ligands L2-L3.

207 ht into the effects of the structures of the phosphoramidite ligands on the stability of the metallac

208 The use of phosphoramidite ligands proved crucial for avoiding comp

209 The synthesis of novel phosphoramidite ligands was critical in this effort, and

210 Using finely tuned phosphoramidite ligands, it is possible to synthesize ei

211 been achieved using [RhCl(coe)2]2 and chiral phosphoramidite ligands.

212 rates, respectively, to the advent of chiral phosphoramidite ligands.

213 of iridium catalysis in concert with N-aryl-phosphoramidite ligands.

214 f synthetic oligonucleotides prepared by the phosphoramidite method.

215 ired the successive use of H-phosphonate and phosphoramidite methods of synthesis.

216 ew terminus modifiers, bearing, along with a phosphoramidite moiety, one, two or four methoxyoxalamid

217 The phosphoramidite monomer of N1-(2,4-dinitrophenyl)-2'-deo

218 o different types of building blocks, i.e. a phosphoramidite monomer that also contains an alkyl brom

219 f the 5'-OH group in conventional nucleotide phosphoramidite monomers (i.e. PGA-gated deprotection),

220 a streamlined method for the preparation of phosphoramidite monomers and a single-step deprotection

221 ort we demonstrate the use of photoprotected phosphoramidite monomers for light-directed array synthe

222 ted in the polymers using different types of phosphoramidite monomers in step (i).

223 NA tether was synthesized with nonnucleotide phosphoramidite monomers using the DNA synthesis chemist

224 This molecular toolkit includes phosphoramidite monomers with Cy3B linked to deoxyribose

225 n of CN(-), leading to a less rigid kappa(2)-phosphoramidite-neutral Pd intermediate.

226 5.0 mol % of a readily available monodentate phosphoramidite-Ni complex in ethanol, affording a varie

227 Here, we report the synthesis of a phosphoramidite of 8-azanebularine and its use in the pr

228 The phosphoramidites of 7 and 14 were prepared, but only the

229 present a solution-phase synthesis based on phosphoramidites of dimers and phenolic cores that produ

230 synthesis of the triphosphates and protected phosphoramidites of these two nucleosides.

231 ioselective allylic substitution enabled by (phosphoramidite,olefin) ligands are reported.

232 described above, each of the eight possible phosphoramidite oligonucleotide building blocks (DE-1/DE

233 -CNG- sequence were prepared using either a phosphoramidite or convertible nucleoside approach.

234 at can be readily converted to nucleoside 2'-phosphoramidites or 3'-triphosphates for solid-phase and

235 n approach is described, which is based on a phosphoramidite (P-amidite) derived reagent (c-PyPA) obt

236 mer extension products, in which acid-labile phosphoramidite (P-N) bonds replace the 5' phosphodieste

237 hase syntheses, uses no more than 4 equiv of phosphoramidite per phenolic alcohol, and provides routi

238 ich is readily incorporated into RNA via the phosphoramidite, perform well at the guide strand 5'-end

239 nsformation remains unclear, both Rh and the phosphoramidite play a central role.

240 l, catalyzed by using axially chiral iridium phosphoramidites PR/S-Ir and cinchona amine is establish

241 methoxytrityl)thymidine N,N-diisopropylamino phosphoramidites protected at P(III) with derivatives of

242 Phosphoramidite-protected quadracyclic 2'-deoxyadenosine

243 polyphosphates were prepared using iterative phosphoramidite protocols on a polystyrene solid support

244 efficiently use smaller volumes of the probe phosphoramidite, purification of pteridine-containing se

245 sis support (2) and a commercially available phosphoramidite reagent (3).

246 -myo-inositol key intermediates, a versatile phosphoramidite reagent was employed in the synthesis of

247 The phosphoramidite reagent was then used to synthesize the

248 ere prepared using a novel N(4)C-ethyl-N(4)C phosphoramidite reagent.

249 ed DNA synthesis and an acetal-protected BAL phosphoramidite reagent.

250 epared using a dimethylformamidine-protected phosphoramidite reagent.

251 Three different diene phosphoramidite reagents have been synthesized that enab

252 urated imines and isocyanates catalyzed by a phosphoramidite-rhodium complex provides pyrimidinones i

253 entration characterizes the number of active phosphoramidite sites on the solid support.

254 onadditive pathway passes through a kappa(2)-phosphoramidite-stabilized Pd(+) intermediate, resulting

255 sis of 2'-O-(o-nitrobenzyl)-3'-thioguanosine phosphoramidite starting from N(2)-isobutyrylguanosine i

256 deoxyguanosine, LdG] embedded DNAs utilizing phosphoramidite strategy.

257 sulfinyl group for 5'-hydroxyl protection of phosphoramidites, such as 10a-d, may lead to the product

258 ile dimethoxytrityl protecting groups of DNA phosphoramidite synthesis reagents with the requisite sp

259 ents are pure chemical species prepared from phosphoramidites synthesized in a single chemical step f

260 We have developed a new phosphoramidite synthon--the alkynyl phosphinoamidite, w

261 method has been demonstrated by introducing phosphoramidite synthons where N-benzyloxycarbonyl (Z) p

262 dinating pyridine ring into a TADDOL-derived phosphoramidite (TADDOL=alpha,alpha,alpha,alpha-tetraary

263 tinum complexes, including hydroxy-tethered, phosphoramidite-tethered, and monodeoxyribonucleotide-te

264 yrene-labeled P4-P6 was prepared using a new phosphoramidite that allows high-yield automated synthes

265 ere we describe the synthesis of a thymidine phosphoramidite that has the 5'-tethered aldehyde masked

266 The triol is introduced via a phosphoramidite that is compatible with standard oligonu

267 Here, we used a phosphoramidite that permits the introduction of GuaUre-

268 meric ligands led to the identification of a phosphoramidite that provided the amination product with

269 ols for 2'-N-methyl-2'-amino-2'-deoxyuridine phosphoramidites that are functionalized at the N2'-posi

270 motivated the development of ribonucleoside phosphoramidites that would exhibit coupling kinetics an

271 iminated by using unequal ratios of the four phosphoramidites, the use of such mixtures does not ensu

272 ted with a known amount of polystyrene-bound phosphoramidite to form a diisopropylamine-DCI salt comp

273 The coupling of 2-cyanoethyl thymidine phosphoramidite to solid-support-bound, phosphate-unprot

274 ions, coupling of each 2'-deoxynucleoside 3'-phosphoramidite to the growing oligodeoxynucleotide on t

275 ed a synthetic method for producing glycerol phosphoramidites to create a covalent 10-mer of (poly)gl

276 We have used 5'-5' phosphoramidites to design reverse-polarity tethers that

277 yl protecting groups for deoxyribonucleoside phosphoramidites to improve the synthesis of oligonucleo

278 n with pseudouridine (Psi) and standard base phosphoramidites to synthesize a 19-nucleotide RNA repre

279 We also describe the use of the protected phosphoramidites to synthesize DNA oligonucleotides cont

280 hesis of biophosphates, catalytic asymmetric phosphoramidite transfer remains largely unexplored for

281 oduced by first incorporating a cross-linked phosphoramidite unit at the 5'-end of an oligonucleotide

282 A new phosphoramidite was identified to be an effective chiral

283 The m(3)Psi phosphoramidite was used in combination with pseudouridi

284 The synthesis of the nucleoside phosphoramidites was accomplished in multigram quantitie

285 e, adenine, and guanine) nucleobase-modified phosphoramidites was accomplished on a multigram scale.

286 Synthesis of the FHNA and Ara-FHNA thymine phosphoramidites was efficiently accomplished starting f

287 The synthesis of the cMOE and cEt nucleoside phosphoramidites was efficiently accomplished starting f

288 ermodified nucleosides and for pseudouridine phosphoramidite were all greater than 98%.

289 -2'-O-methyl uridine and cytidine nucleoside phosphoramidites were achieved using glucose as the star

290 sphoramidites and mixtures of diastereomeric phosphoramidites were evaluated in the iridium-catalyzed

291 These phosphoramidites were incorporated into oligonucleotides

292 Phosphoramidites were prepared and employed in solid-pha

293 ly diverse C5-functionalized LNA uridine (U) phosphoramidites were synthesized and incorporated into

294 8-Alkoxyadenosine phosphoramidites were synthesized and incorporated into

295 Phosphoramidites were synthesized bearing clickable 2'-O

296 ning Fapy.dG utilized a reverse dinucleotide phosphoramidite, which also required the synthesis of th

297 The new phosphoramidites will be particularly valuable for enabl

298 tetrazole-promoted coupling of a cytidine-5'-phosphoramidite with a glycosyl thiol of a protected sia

299 We have synthesized two novel phosphoramidites with a ferrocenyl moiety at the 2'-ribo

300 Metal-containing phosphoramidites with predetermined absolute configurati