ライフサイエンスコーパス: phosphoserine

1 verted the phosphohydroxypyruvate product to phosphoserine.

2 ference for basic residues N-terminal to the phosphoserine.

3 deleted activity by catalyzing hydrolysis of phosphoserine.

4 trains in which UAG was reassigned to encode phosphoserine.

5 tuned by incorporation of dimethyllysine or phosphoserine.

6 had an added mass of 170amu to form o-cresyl phosphoserine.

7 as 24 different residues, including 7 major phosphoserines.

8 high density of modified lysine residues and phosphoserines.

9 s screened with an antibody directed against phosphoserine 1 of H4.

10 We report that phosphoserine 112 (pSer112) dephosphorylation functions

11 Colocalization was observed with the phosphoserine-15 form of p53 at presumed DNA processing

12 irradiation-induced MAPK activation and p53, phosphoserine-15 p53, as well as induced p21 and DDR1 le

13 Whereas levels of p53, phosphoserine-15 p53, p21, ARF and Bcl-X(L) were increas

14 Pol II, suggesting that all polymerase with phosphoserine 2 also contains phosphoserine 5.

15 vity with monoclonal antibodies specific for phosphoserine 2 or phosphoserine 5.

16 that there was a pronounced decrease in the phosphoserine-2 form and in overall RNAP II levels in ly

17 nd lactacystin prevented the decrease in the phosphoserine-2 form and in overall RNAP II levels; howe

18 Further, there was an overall reduction in phosphoserine-2 staining.

19 kinase (CaMK)-dependent binding of 14-3-3 to phosphoserines 259 and 498 activates the NES, with conse

20 clic AMP-dependent protein kinase (PKA) site phosphoserine 262 and the G protein-coupled receptor kin

21 Both species were detected by a Bcr phosphoserine 354 sequence-specific antibody.

22 G protein-coupled receptor kinase (GRK) site phosphoserines 355 and 356 of the beta2-adrenergic recep

23 if and a short basic motif (SBM) adjacent to phosphoserine-366.

24 ntibody, KOR-P, that specifically recognized phosphoserine 369 KOR.

25 phosphospecific antibodies directed against phosphoserine 383 and phosphoserine 387.

26 odies directed against phosphoserine 383 and phosphoserine 387.

27 n 5- and 11-fold, with parallel decreases in phosphoserine-432.

28 Given reports indicating that phosphoserine 5 is present during elongation in yeast, o

29 In support of this conclusion, a phosphoserine 5-specific antibody quantitatively immunop

30 olymerase with phosphoserine 2 also contains phosphoserine 5.

31 l antibodies specific for phosphoserine 2 or phosphoserine 5.

32 A phosphoserine 523 antibody revealed that Ser523 is rapid

33 sing a phosphospecific antibody we show that phosphoserine-54 Cdc6 maintains a high affinity for chro

34 nstrate that PTN stimulates translocation of phosphoserine 713 and 726 beta-adducin either to nuclei,

35 canonical phosphodegron in Cdc25A containing phosphoserine 79 and phosphoserine 82, sites that are no

36 on in Cdc25A containing phosphoserine 79 and phosphoserine 82, sites that are not targeted by Chk1.

37 Moreover, CAD phosphoserine, a measure of PKA phosphorylation, increas

38 1 decapeptide (BDP1) containing the critical phosphoserine, a phenylalanine at (P+3), and a GST-BRCT

39 droxyphenyl-L-alanine (DOPA) and serine to O-phosphoserine accounts for the hydroxylation and phospho

40 PI3K protein, phosphoserine Akt, and p42/p44-MAPK were reduced, howeve

41 the transamination of aspartate, glutamate, phosphoserine, alanine, and cysteate.

42 s phosphoglycerate dehydrogenase (PHGDH) and phosphoserine aminotransferase 1 (PSAT-1), were recently

43 way (phosphoglycerate dehydrogenase (PHGDH), phosphoserine aminotransferase 1 (PSAT1), and phosphoser

44 n to have phosphoglycerate dehydrogenase and phosphoserine aminotransferase activities.

45 Measurement of phosphoserine aminotransferase activity in cultured fibr

46 We present the first two identified cases of phosphoserine aminotransferase deficiency.

47 This gene encodes phosphoserine aminotransferase, the enzyme for the secon

48 esis (Phosphoglycerate dehydrogenase, Phgdh; phosphoserine aminotransferase-1, Psat1) following treat

49 nted a serC mutation in the Escherichia coli phosphoserine aminotransferase.

50 strating that M. tuberculosis serC encodes a phosphoserine aminotransferase.

51 nthesis, because the expression of PGDH1 and phosphoserine aminotransferase1 is regulated by MYB51 an

52 data indicating that 14-3-3 binding requires phosphoserine and +2 proline).

53 fetuin and MGP, contains several residues of phosphoserine and accumulates in bone.

54 ine], but also C18:1/OH lyso-PLs bearing the phosphoserine and phosphoethanolamine head groups, prese

55 and 1080cm(-1) in solution Raman spectra of phosphoserine and phosphothreonine are assigned to the m

56 We found that rWip1 dephosphorylates phosphoserine and phosphothreonine in the p(S/T)Q motif,

57 ence of the atypically high pK values of the phosphoserine and phosphothreonine residues and the pref

58 ein phosphatases (PPPs) that dephosphorylate phosphoserine and phosphothreonine residues are increasi

59 Phosphoserine and phosphothreonine residues exhibited or

60 e-induced beta-elimination of phosphate from phosphoserine and phosphothreonine residues followed by

61 ever, loss of the phosphate moiety from both phosphoserine and phosphothreonine residues in low-energ

62 is the selective chemical transformation of phosphoserine and phosphothreonine residues into lysine

63 , followed by chemical derivatization of the phosphoserine and phosphothreonine residues using stable

64 sually long kinase insert loop with multiple phosphoserine and phosphothreonine residues.

65 yperphosphorylated Nup species revealed only phosphoserine and phosphothreonine residues.

66 1 is a PP2C phosphatase and dephosphorylates phosphoserine and phosphothreonine residues.

67 in the presence of N-Fmoc and O-Et protected phosphoserine and phosphotyrosine to prepare molecularly

68 acid 2-phosphate, glyceric acid 3-phosphate, phosphoserine and pyruvate.

69 ecrease IRF5 in Tsk(-/+) hearts and decrease phosphoserine and ubiquitin K(63) but increase total ubi

70 Phosphoserine and ubiquitinylated beta-catenin were dete

71 sacylation of tRNA(Cys) with d-cysteine or O-phosphoserine and upon changing the protonation state of

72 Additional salt bridges between the phosphoserines and SUMO accounted for the increased IE2-

73 termine the corresponding pKa values of 5.6 (phosphoserine) and 5.9 (phosphothreonine).

74 ine), SOPS (1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine), and DPPtdIns(3)P (1,2-dipalmitoylphospha

75 ding the phosphorylation site of HSP20, 2) a phosphoserine, and 3) a protein transduction domain.

76 u(2+), and V(2+) were reacted with HPO4(2-), phosphoserine, and a phosphopeptide (FQpSEEQQQTEDELQDK,

77 ands, namely, chloride, inorganic phosphate, phosphoserine, and phosphorylated CTD peptides.

78 of human tRNA(Sec) in complex with SepSecS, phosphoserine, and thiophosphate, together with in vivo

79 those serines with aspartic acids, mimics of phosphoserines, and investigated the properties of the c

80 by BeWo nuclear extracts are supershifted by phosphoserine- and phosphothreonine- but not phosphotyro

81 d used in silico docking methods to identify phosphoserine- and phosphotyrosine-containing peptides a

82 bacterial PPPs, the enzyme dephosphorylated phosphoserine- and phosphotyrosine-containing proteins w

83 The BRCT repeats in BRCA1 are phosphoserine- and/or phosphothreonine-specific binding

84 ting using either anti-PKA substrate or anti-phosphoserine antibodies.

85 of radiolabeled phosphate, detection by anti-phosphoserine antiserum, and the stabilizing effect of g

86 rent properties such as very high content of phosphoserine ( approximately 270 Ser(P) residues/1000 a

87 fer the phosphoryl group to homoserine using phosphoserine as the phosphoryl group donor, indicating

88 Peptides with phosphoserine at either the P-1 or P-2 position experien

89 phorylation sites identified, two C-terminal phosphoserines at positions 1938 and 1989 showed increas

90 w also discusses our findings that show that phosphoserine Bcr by means of a unique structure, binds

91 rine kinase activity, yielding predominantly phosphoserine Bcr, despite the presence of Bcr-Abl in th

92 mechanism critical for regulating WW domain phosphoserine binding activity and Pin1 function.

93 1 binds a domain of 14-3-3 distinct from the phosphoserine binding pocket.

94 activation results in the recruitment of the phosphoserine binding protein 14-3-3epsilon in a manner

95 tions only in the Est1 14-3-3-like domain, a phosphoserine-binding motif, the first example of a 14-3

96 The composition and structure of the BARD1 phosphoserine-binding pocket P1 are strikingly similar t

97 We now show that 14-3-3 tau, a phosphoserine-binding protein, mediates E2F1 stabilizati

98 3-3 family members are intracellular dimeric phosphoserine-binding proteins that regulate signal tran

99 14-3-3 family members are dimeric phosphoserine-binding proteins that regulate signal tran

100 14-3-3 proteins are phosphoserine-binding proteins.

101 sions, and covalent protein-DNA linkages via phosphoserine bonds.

102 NMR and crystallography indicate that a phosphoserine, but not a phosphomimetic, competes for a

103 d the resulting seryl moiety is converted to phosphoserine by O-phosphoseryl-tRNA kinase (PSTK) in eu

104 which tRNA(Cys) is first aminoacylated with phosphoserine by phosphoseryl-tRNA synthetase (SepRS).

105 ults suggest that proper presentation of the phosphoserine can greatly affect the rate of dephosphory

106 ; second, following dephosphorylation of the phosphoserine cluster (Ser202 and Ser208), Phox2a become

107 We found that phosphoserine-containing diphosphorylated peptides with

108 tative 14-3-3 interaction sites, including a phosphoserine-containing motif that directly binds to 14

109 A phosphoserine-containing peptide was identified from try

110 Alpha-ZrPN were able specifically to capture phosphoserine-containing peptides from a tryptic digest

111 y reduction for fast and focused analysis of phosphoserine-containing peptides.

112 BARD1, and DNA Ligase IV preferred distinct phosphoserine-containing peptides.

113 p which rapidly pinpointed the original four phosphoserine-containing peptides.

114 dephosphorylated phosphotyrosine- as well as phosphoserine-containing proteins in vitro.

115 AG codon we produce site-specific serine- or phosphoserine-containing proteins, with purities approac

116 with feedback control of B55 activity by the phosphoserine-containing substrate/inhibitor ENSA, can h

117 mulate ERK1/ERK2 activation and increase the phosphoserine content of KSR, which are inhibited by kiK

118 Phosphoserine content of PTP1B declined in response to i

119 arp decline in PTP1B activity, a decrease in phosphoserine content, and an increase in PTP1B phosphot

120 eptides of identical amino acid sequence and phosphoserine content, which differed only in the positi

121 es, confirmed that the aromatic residues and phosphoserines contribute to the formation of a dynamic

122 ge in the enzyme's active site that allows a phosphoserine covalently attached to tRNA(Sec), but not

123 E-selectin surface expression appears to be phosphoserine dependent, since alanine but not aspartic

124 ith the protein kinase inhibitor UCN-01 in a phosphoserine-dependent manner.

125 Like 14-3-3, SMG7 engages phosphoserine-dependent protein interactions; however, t

126 Unexpectedly, loss of phosphoserine-dependent SMG7 binding does not significan

127 icate that gamma functions autonomously as a phosphoserine-dependent transrepressor to downregulate t

128 overcome the intrinsically low preference of phosphoserine for the interior of the helix.

129 tly share this pathway, providing sufficient phosphoserine for the tRNA-dependent cysteine biosynthet

130 pression of BCR in BCR-ABL+ cells produces a phosphoserine form of Bcr, which inhibits the oncogenic

131 This altered phosphoserine form of the Bcr protein selectively binds

132 .e. it is not dependent on the presence of a phosphoserine, four residues C-terminal to the GSK-3 pho

133 ze human MEK1 kinase with two serines or two phosphoserines, from one DNA template, and demonstrate p

134 Altered surface electrostatics from the phosphoserine group disrupt its intramolecular associati

135 The side chain of the phosphoserine group does not interact with the VHS domai

136 compounds that afford neutral or monoanionic phosphoserine groups thereby indicating a high specifici

137 600-fold), phosphotyrosine (>8000-fold), and phosphoserine (>8000-fold).

138 Stabilization of the phosphoserine headgroup and glycerol backbone of C(6)PS

139 roup donor, indicating that ThrH has a novel phosphoserine:homoserine phosphotransferase activity.

140 st deoxyribozyme decreases the half-life for phosphoserine hydrolysis from as high as >10(10) y to <1

141 ed a 4.8-fold increase of CLASP2 in the anti-phosphoserine immunoprecipitates upon insulin stimulatio

142 We detected a significant elevation of phosphoserine in frontal cortical D(1A)DARs of rabbits t

143 t that the ZAP-70 PTK contains predominately phosphoserine in normal T lymphocytes as well as in Jurk

144 I13G) and at a site that interacts with the phosphoserine in phosphorylase a, (R69K and R69E).

145 Yet Sgg and Dbt often require a phosphoserine in their target site, and analysis of Per

146 of aromatic residues and their synergy with phosphoserines in an intrinsically disordered regulatory

147 the phosphatase activity is directed to five phosphoserines in the amino-terminal activation function

148 Ablation of this phosphoserine interaction produces developmental defects

149 ry switch in the H3-HP1 system, but arginine-phosphoserine interactions, which occur in both histones

150 ast, inclusion of 1,2-dilauroyl-sn-glycero-3-phosphoserine into diC(12:0)PC liposomes resulted in a d

151 -specific, co-translational incorporation of phosphoserine into proteins.

152 e, benzoyl-phenylalanine, acetyl-lysine, and phosphoserine into selected Salmonella proteins includin

153 roteins bound to a 1,2-dioleoyl-sn-glycero-3-phosphoserine lipid bilayer.

154 -3-3 binding site (RSXpSXP, where pS denotes phosphoserine) located in the amino-terminal region of H

155 d counterparts, as well as acetyl-lysine and phosphoserine marks.

156 "go, no-go" step for autophagic fusion via a phosphoserine master-switch.

157 blastin ( Ambn)-knockout mice, implying that phosphoserines may have a critical role in AMBN function

158 c domain associated with 14-3-3beta by a non-phosphoserine mechanism.

159 ional distinct regulatory mechanisms besides phosphoserine-mediated protein interactions.

160 duced STAT1 S727 phosphorylation by >30% and phosphoserine-mediated transcriptional activity by 58% b

161 Similarly, when Ser730 was replaced by the phosphoserine mimetic, Asp, cleavage secretion of the re

162 Substitution of both residues with the phosphoserine mimetic, aspartic acid, produced a mutant

163 cyclic phosphopeptide with a nonhydrolyzable phosphoserine mimetic.

164 In contrast, replacing serine with phosphoserine-mimetic aspartic acid (S386D) in ANDV N ro

165 nine (Ser(312) --> Ala beta(1)-AR) or to the phosphoserine mimic aspartic acid (Ser(312) --> Asp beta

166 nit of PKA or mutagenesis of Ser(312) to the phosphoserine mimic aspartic acid both rescued the recyc

167 Utilizing phosphoserine-mimicking Ser-to-Glu point mutations, the

168 However, whether this receptor phosphoserine mode of signaling is able to regulate spec

169 hE from the sample matrixes by binding their phosphoserine moieties that were exposed through unfoldi

170 nature within the CCTalpha carboxyl-terminal phosphoserine motif (residues 328-343).

171 Phosphoserine occurs in sequences strikingly reminiscent

172 nt of electrostatic interactions between the phosphoserines of RAP80 and the SIM recognition module w

173 ron appeared to interact simultaneously with phosphoserine on the caseins and inorganic phosphorus.

174 We identified a novel phosphoserine on the dynein intermediate chains (ICs), a

175 Several protein domains that bind phosphoserine or phosphothreonine residues have been ide

176 ions at these m/z values for the presence of phosphoserine or phosphothreonine residues using tandem

177 tion to the primary sequence surrounding the phosphoserine or threonine.

178 Cellular PPIase Pin1 binds specifically to phosphoserine- or phosphothreonine-proline (pS/T-P) moti

179 domain-interacting protein) and in BRCA1 as phosphoserine- or phosphothreonine-specific binding modu

180 , we measured the fidelity and purity of the phosphoserine orthogonal translation system (OTS) and us

181 It does not attack d-serine, l-phosphoserine, other l-amino acids, or phosphatidylserin

182 ossesses a strong and unusual preference for phosphoserine over phosphothreonine at Pro-directed site

183 d Scp1 belong to a family of Mg2+ -dependent phosphoserine (P.Ser)/phosphothreonine (P.Thr)-specific

184 structure of the complex between memapsin 2 phosphoserine peptide and GGA1 VHS was solved at 2.6 A r

185 e specificity of the CyPAA ion for detecting phosphoserine peptides in complex peptide mixtures is co

186 s reported to modify the phosphate groups on phosphoserine peptides to the corresponding phosphoramid

187 l structure of the complex of BeF(3)(-) with phosphoserine phosphatase (PSP) from Methanococcus janna

188 Phosphoserine phosphatase (PSP) is a member of a large c

189 ncoding a multidomain enzyme with a putative phosphoserine phosphatase (PSP) motif fused to glycerol-

190 Phosphoserine phosphatase (PSP), a key essential metabol

191 (PPSB) in Arabidopsis thaliana by targeting phosphoserine phosphatase (PSP1), the last enzyme of the

192 hosphoserine aminotransferase 1 (PSAT1), and phosphoserine phosphatase (PSPH)) and de novo glycine sy

193 th homoserine kinase (thrB gene product) and phosphoserine phosphatase (serB gene product) activities

194 otyrosine phosphatase activity and decreased phosphoserine phosphatase activity.

195 Furthermore, analysis of pgdh1 and phosphoserine phosphatase mutants revealed an embryo-let

196 eriodontal pathogen, secretes the HAD family phosphoserine phosphatase SerB653 when in contact with g

197 d in vitro assays show that ThrH is indeed a phosphoserine phosphatase with a K(m) of 0.207 mm and k(

198 By comparison to phosphoserine phosphatase, a well-studied member of this

199 escuer of cells in which serB, which encodes phosphoserine phosphatase, an enzyme essential for serin

200 imilarities in mutant phenotypes of Tpp1 and phosphoserine phosphatase, we propose a reaction mechani

201 ameshift mutation in PSPH, the gene encoding phosphoserine phosphatase, which catalyzes the last step

202 aternal embryonic leucine zipper kinase, and phosphoserine phosphatase, with variation from tumor to

203 asmic Ca(2+)-ATPase, phosphomannomutase, and phosphoserine phosphatase.

204 erexpressed, compensates for the deletion of phosphoserine phosphatase.

205 parison has revealed that ThrH is related to phosphoserine phosphatases (PSP, EC 3.1.3.3) and belongs

206 sm for P5N-1 that is also similar to that of phosphoserine phosphatases and provide experimental evid

207 a metal-dependent reaction analogous to the phosphoserine phosphatases of the haloacid dehalogenase

208 e is structurally highly similar to those of phosphoserine phosphatases.

209 ed mAb scaffolds with hot spots specific for phosphoserine, phosphothreonine or phosphotyrosine.

210 gnal intensities in immunoblotting with anti-phosphoserine/phosphothreonine antibodies.

211 ch is defined as a 14-3-3 interaction with a phosphoserine/phosphothreonine at the C-termini of the t

212 of this residue with a non-hydrolyzable the phosphoserine/phosphothreonine mimetic would promote bin

213 Human PP2Calpha is a metal-dependent phosphoserine/phosphothreonine protein phosphatase and i

214 ultaneously and synchronously inactivate all phosphoserine/phosphothreonine-binding domain family mem

215 urrent implementation performs validation of phosphoserine/phosphothreonine-containing peptides havin

216 ion ( approximately 10-15%) of the intrinsic phosphoserine present in the plasma-derived FVa heavy ch

217 We show that these phosphoserines prime further phosphorylation at adjacent

218 To establish the mode of phosphoserine production in Methanococcales, cell extrac

219 We identified a cluster of phosphoserine-proline sites in Phox2a by mass spectromet

220 ease digestion, differential reactivity to a phosphoserine-proline-directed monoclonal antibody (MPM-

221 ing peptides but exhibits no activity toward phosphoserine (pS) or phosphothreonine (pT) peptides.

222 athways, the photorespiratory and glycolytic phosphoserine (PS) pathways, have been postulated.

223 brain, we identified in each sample in vivo phosphoserine (pS) phosphorylation sites at pS434, pS440

224 nase polo-like kinase 1 (Plk1) as a specific phosphoserine (pSer) or phosphothreonine (pThr) binding

225 ns with short peptides composed of repeating phosphoserine (pSer) residues enhances binding to alum a

226 n(2+)/Mn(2+)-dependent formation of Dha from phosphoserine (pSer), i.e., exhibit pSer lyase activity,

227 resulted in Ca(2+) complexation by H-fibroin phosphoserines (pSs) and a shift in secondary structure

228 N-terminal primary sequence surrounding the phosphoserine (R16A, R16E, and I13G) and at a site that

229 ugh a primitive SH2 domain, which recognizes phosphoserine rather than phosphotyrosine residues.

230 Building upon previous studies of the phosphoserine residue (pSer), we address the distinct na

231 The role of the phosphoserine residue and its effects on alpha-syn struc

232 ing an uncommon 3-carbamoyl threonine, and a phosphoserine residue in celebesides A and B.

233 ts that the intrinsic nucleophilicity of the phosphoserine residue is much higher than previously app

234 One phosphoserine residue is present within the gamma domain

235 By incorporating a caged phosphoserine residue within a consensus motif, these re

236 odel peptide FQpSEEQQQTEDELQDK, containing a phosphoserine residue, was converted to EA-tuned peptide

237 nohistochemistry with antibodies against the phosphoserine residues in both S6rp and 4E binding prote

238 uced the conversion efficiency of one of the phosphoserine residues of ovalbumin, suggesting a role f

239 protein overlay studies established that the phosphoserine residues, located at the C terminus of CmR

240 lanine (Dha) in place of phosphothreonine or phosphoserine residues, respectively.

241 14-3-3 proteins bind to specific phosphoserine residues.

242 rsely the anti-AChE10SP antiserum recognized phosphoserine rMoAChE that resulted from reaction with p

243 rchaeal protein to serine is consistent with phosphoserine's position as a branch point in several pa

244 onine binding and is largely responsible for phosphoserine selectivity.

245 cated that tRNA(Cys) becomes acylated with O-phosphoserine (Sep) but not with cysteine.

246 A(Cys) in a two-step pathway, first charging phosphoserine (Sep) onto tRNA(Cys) and subsequently conv

247 We first use the established O-phosphoserine (Sep) orthogonal translation system to cre

248 atalyzes the sulfhydrylation of tRNA-bound O-phosphoserine (Sep) to form cysteinyl-tRNA(Cys) (Cys-tRN

249 ires the tRNA(Sec)-dependent conversion of O-phosphoserine (Sep) to Sec.

250 S) catalyzes the ligation of a mismatching O-phosphoserine (Sep) to tRNA(Cys) followed by the convers

251 ryl-tRNA synthetase charges tRNA(Cys) with O-phosphoserine (Sep), a precursor of the cognate amino ac

252 etase (SepRS), which acylates tRNA(Cys) with phosphoserine (Sep), and the well known cysteinyl-tRNA s

253 O-Phosphoserine (Sep), the most abundant phosphoamino acid

254 deduced from the cement to be about 80 mol % phosphoserine/serine, and the cDNA was obtained by explo

255 Sucrose synthase (SS) is a known phosphoserine (SerP)-containing enzyme in a variety of p

256 eractions between adjacent methyl-lysine and phosphoserine side chains do not by themselves provide a

257 Phosphoserine-specific antibodies demonstrate that K8 Se

258 ney (HEK) 293 cells were characterized using phosphoserine-specific antibodies.

259 ophoretic mobility was found to react with a phosphoserine-specific antibody mainly in the PIKfyveWT-

260 Using a phosphoserine-specific antibody, we show that the associ

261 phopeptide mapping and immunoblotting with a phosphoserine-specific PKC substrate antibody, revealed

262 g, autoradiography, and immunoblotting using phosphoserine-specific titin-antibodies.

263 he Cdc14 substrate Acm1, we demonstrate that phosphoserine specificity exists in vivo.

264 s this adenosine suppressive effect on STAT1 phosphoserine status by 25-50%.

265 prior to substrate binding, the complex with phosphoserine substrate bound (with a D to N mutation in

266 choline and 1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine supported on a buffered aqueous solution.

267 inal end of the RS domain generates a primed phosphoserine that binds to a basic site in the kinase.

268 With the serine residue replaced by phosphoserine, the K(d) decreased about 10-, 4-, and 14-

269 We demonstrate that the phosphoserine, -threonine or -tyrosine, interaction prot

270 tor kinase complex Dbf2-Mob1 bound through a phosphoserine-threonine binding domain, in order to be a

271 Plk1 contains a specialized phosphoserine-threonine binding domain, the Polo-box dom

272 nd GAD67 and immunoblotting assay using anti-phosphoserine/threonine antibodies.

273 between Cdc25C phosphatase and 14-3-3epsilon phosphoserine/threonine binding protein.

274 cient hydrolysis of both phosphotyrosine and phosphoserine/threonine found on signaling proteins, as

275 e, particularly in light of the discovery of phosphoserine/threonine peptide motifs as binding target

276 KC-mediated phosphorylation of UGT such that phosphoserine/threonine regulates substrate specificity

277 hat dephosphorylate both phosphotyrosine and phosphoserine/threonine residues in vitro.

278 tyrosine-labeled peptides tested but not the phosphoserine/threonine residues on casein and histone.

279 ike kinase 1, a mitotic kinase that binds to phosphoserine/threonine through its polo-box domain (PBD

280 nse to ligand and binds the 14-3-3 family of phosphoserine/threonine-binding adaptor/scaffold protein

281 family contain the conserved 14-3-3 protein phosphoserine/threonine-binding consensus motif.

282 th Polo-boxes, functions as a single modular phosphoserine/threonine-binding domain known as the Polo

283 of the DNA-dependent protein kinase, and the phosphoserine/threonine-binding protein 14-3-3 zeta.

284 14-3-3 proteins are phosphoserine/threonine-binding proteins that play impor

285 silon (YWHAE), one of a family of ubiquitous phosphoserine/threonine-binding proteins, is always dele

286 enopus, and yeast Plks all recognize similar phosphoserine/threonine-containing motifs.

287 Here, we describe a method for enriching phosphoserine/threonine-containing proteins from crude c

288 hosphotyrosine antibodies, the enrichment of phosphoserine/threonine-containing proteins has not been

289 key connection linking phosphotyrosine- and phosphoserine/threonine-mediated oncogenic signals.

290 The 14-3-3 family of phosphoserine/threonine-recognition proteins engage mult

291 valently attached to tRNA(Sec), but not free phosphoserine, to be oriented properly for the reaction

292 Phosphoserine, ubiquitin, ubiquitin K(63) on IRF5 were d

293 tide by 3H counting, and the location of the phosphoserine was defined by the N-terminal Edman degrad

294 The specific phosphoserines were identified to be Ser(56), Ser(58), a

295 c residue at the +3 position relative to the phosphoserine, whereas substrates lacking this basic res

296 hosphatidylserine, glycerophosphoserine, and phosphoserine, which are not transported by the plasma m

297 These methanogens charge tRNA(Cys) with l-phosphoserine, which is also an intermediate in the pred

298 o acid analysis demonstrates the presence of phosphoserine within Arix.

299 ent, which differed only in the positions of phosphoserine within the heptad, we found that Fcp1 was

300 ssential protein phosphatase that hydrolyzes phosphoserines within the C-terminal domain (CTD) of the