ライフサイエンスコーパス: photobleaching

1 -dependent reactions, which usually cause PS photobleaching.

2 rature-dependent fluorescence recovery after photobleaching.

3 ation, and rapid fluorescence recovery after photobleaching.

4 assumptions about the spectral dependency of photobleaching.

5 intensity, the excess intensity just adds to photobleaching.

6 ed with PPIX fluorescence and degree of PPIX photobleaching.

7 s needs to be considered when correcting for photobleaching.

8 ements for finite filament length as well as photobleaching.

9 heir different fluorescence stability during photobleaching.

10 movement, low signal-to-background ratio and photobleaching.

11 spectroscopy and fluorescence recovery after photobleaching.

12 dic according to fluorescence recovery after photobleaching.

13 by the quantum yield of fluorophores and by photobleaching.

14 acteria cells achieved by regular SIRM after photobleaching.

15 asurements using fluorescence recovery after photobleaching.

16 aneously measuring fluorescence lifetime and photobleaching.

17 this artifact was measured using single-step photobleaching.

18 cle fusion using fluorescence recovery after photobleaching.

19 s analyzed using fluorescence recovery after photobleaching.

20 uper-resolution imaging with greatly reduced photobleaching.

21 itive and susceptible to photoinhibition and photobleaching.

22 polyp expansion, coral tissue reaction, and photobleaching.

23 ter cellular behavior and are susceptible to photobleaching.

24 uantified, using fluorescence recovery after photobleaching.

25 s to avoid photodamage to the cell and rapid photobleaching.

26 spectroscopy and fluorescence recovery after photobleaching.

27 s and generated a small fraction of opsin by photobleaching ~1% of rhodopsin.

28 applied a combination of biochemical assays, photobleaching/activation approaches, and atomistic mole

29 itions within a nucleus, without significant photobleaching, allowing us to make reliable estimates o

30 Additionally, fluorescence recovery after photobleaching analysis indicated impaired vimentin dyna

31 Fluorescence recovery after photobleaching analysis indicates that the paranodes pro

32 rgy transfer and fluorescence recovery after photobleaching analysis of postischemic brain endothelia

33 Interestingly, fluorescence recovery after photobleaching analysis reveals differential mobility of

34 trated in vitro, fluorescence recovery after photobleaching analysis suggests interactions in vivo ar

35 Here, we use single-molecule photobleaching analysis to measure the probability of Cl

36 Using fluorescence recovery after photobleaching analysis, we first show that secretory ve

37 Using fluorescence recovery after photobleaching analysis, we show that H1.3 has superfast

38 longer fluorescence lifetime, resistance to photobleaching and 10-100 times higher molar extinction

39 nd the ERC using fluorescence recovery after photobleaching and a novel sterol efflux assay.

40 ability, as evidenced by suppression of both photobleaching and blinking behavior.

41 These probes are non-photobleaching and can be used alongside fluorophores wi

42 s as measured by fluorescence recovery after photobleaching and caused chromosome decondensation simi

43 Secondary outcome measures were PPIX photobleaching and clinical local skin reactions, suppor

44 showed that SiRA is remarkably resistant to photobleaching and constitutes the brightest far-red lig

45 Common pitfalls such as photobleaching and cross-talk are addressed, as well as

46 Based on fluorescence recovery after photobleaching and endocytosis assays, integrin recyclin

47 copy techniques: fluorescence recovery after photobleaching and fluorescence correlation spectroscopy

48 al stress, using fluorescence recovery after photobleaching and fluorescence correlation spectroscopy

49 eurons employing fluorescence recovery after photobleaching and fluorescence correlation spectroscopy

50 The effect of scanning speed on photobleaching and fluorescence yield is more remarkable

51 terizations with fluorescence recovery after photobleaching and FRET corroborate the formation of mul

52 Photobleaching and genetic perturbations showed that the

53 racked in native terminals with simultaneous photobleaching and imaging (SPAIM) to show that DCVs und

54 ) is valuable for its combination of reduced photobleaching and outstanding spatiotemporal resolution

55 ften used as an acceptor but YFP is prone to photobleaching and pH changes.

56 Using photobleaching and pharmacological perturbations in vivo

57 Fluorescence photobleaching and photoactivation experiments also reve

58 Using photobleaching and photoconversion experiments in glial

59 formations, evaluate their stability against photobleaching and photoconversion in the context of oth

60 challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quali

61 nges in biological imaging include labeling, photobleaching and phototoxicity, as well as light scatt

62 ence-based voltage sensors often suffer from photobleaching and phototoxicity, which limit the record

63 To overcome issues with photobleaching and poor distinction between confocal and

64 y, we demonstrate using fluorescence loss in photobleaching and quantitative co-localization with chr

65 sessed by slowed fluorescence recovery after photobleaching and resistance to salt.

66 Meanwhile, the unique photobleaching and signal-to-noise benefits afforded by

67 cytoplasmic oligomerization, while stepwise photobleaching and single molecule colocalization may be

68 ity of the method and the demonstration that photobleaching and the photophysical properties of the d

69 rials are often used for their resistance to photobleaching and their complex viewing-direction-depen

70 tamate uncaging, fluorescence recovery after photobleaching and transgenic mice expressing labeled PS

71 of round cells, fluorescence recovery after photobleaching, and a mathematical mean-field model of c

72 s illumination light to photoswitch off than photobleaching, and can be photoswitched "on" again to r

73 er microsurgery, fluorescence recovery after photobleaching, and fluorescence correlation spectroscop

74 ng of single fluorescent proteins, step-wise photobleaching, and multiparameter spectroscopy, allows

75 ve-cell imaging, fluorescence recovery after photobleaching, and single molecule tracking showed that

76 eling protocols, fluorescence recovery after photobleaching, and single particle tracking.

77 ation was subsequently reduced by additional photobleaching, and the diffusion of individual SRB mole

78 scent microscopy, fluorescent recovery after photobleaching, and transmission electron microscopy, th

79 In contrast, photobleaching anterograde transport vesicles entering a

80 ily because of the difficulty of determining photobleaching apparent quantum yields (AQYs) that captu

81 present a simple method to determine a CDOM photobleaching AQY matrix (AQY-M) for natural water samp

82 ons by employing fluorescence recovery after photobleaching as an in vivo assay to measure the influe

83 obabilities, in conjunction with fluorophore photobleaching assays on over 2000 individual complexes,

84 se PolC functions in B. subtilis, we applied photobleaching-assisted microscopy, three-dimensional su

85 ciation, we used fluorescence recovery after photobleaching beam-size analysis to study the membrane

86 ti-colour emission process, and blinking and photobleaching behaviours of single tetrapods can be con

87 ever, long maturation times, low brightness, photobleaching, broad emission spectra, and sample autof

88 Our results show that photobleaching can critically skew the estimation of oli

89 However, photobleaching can occur before the sample is appropriat

90 hoton excitation (2PE) and poorly understood photobleaching characteristics have made their implement

91 MG complexes emit 2-fold more photons before photobleaching compared to organic dyes such as Cy5 and

92 rwent fusion and fluorescence recovery after photobleaching consistent with the PRD LLPS in vitro.

93 for analysis of fluorescence recovery after photobleaching data.

94 with the dynactin disruptor mycalolide B or photobleaching DCVs entering a synaptic bouton by retrog

95 Fluorescence recovery after photobleaching demonstrates that P(DeltaOD) diffuses 6-f

96 h-RAM computer (64 Gb) to run and includes a photobleaching detrending algorithm, which allows extens

97 tation times, thereby drastically decreasing photobleaching during repeated scanning.

98 hen fractional mislabeling occurs as well as photobleaching during the imaging process, and reveals i

99 enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP) of NADH has been shown to be an

100 No notable photobleaching effect or degradation could be observed d

101 Our approach is based on cytochrome photobleaching effects observed in the resonance Raman s

102 these MgPzs (with the highest and the lowest photobleaching efficiencies), we found that the higher t

103 integrin tensions and can be switched off by photobleaching, enabling continuous real-time imaging of

104 Photobleaching event counting is a single-molecule fluor

105 uorophores, and the possibility that several photobleaching events occur almost simultaneously.

106 ddition to complications such as overlapping photobleaching events that may arise from fluorophore in

107 e microscopy and fluorescence recovery after photobleaching experiments and found that mycomembrane f

108 Fluorescence recovery after photobleaching experiments demonstrated that subunit exc

109 te, we performed fluorescence recovery after photobleaching experiments in living cells, which expres

110 in reporters and fluorescence recovery after photobleaching experiments in zebrafish embryos identifi

111 Photobleaching experiments indicated that co-assembly of

112 Mechanistically, fluorescence-recovery-after-photobleaching experiments point for the upstream role o

113 Fluorescence-recovery-after-photobleaching experiments revealed that Kv2.1, VAPA, an

114 Single-molecule fluorescence photobleaching experiments revealed that PopD formed mos

115 gth control, but fluorescence recovery after photobleaching experiments rule out the initial bolus mo

116 These data, together with photobleaching experiments to measure nucleolar protein

117 In summary, any inference from photobleaching experiments with B > 0.1 is likely to be

118 Through fluorescence recovery after photobleaching experiments, many components of the AMR h

119 complemented by fluorescence recovery after photobleaching experiments, which reveal an inverse corr

120 ere monitored by fluorescence recovery after photobleaching experiments.

121 pool with kinetics similar to those seen in photobleaching experiments.

122 id dynamics with fluorescence recovery after photobleaching experiments.

123 Fluorescence loss in photobleaching (FLIP) and network analysis experiments r

124 is combined with fluorescence recovery after photobleaching, fluorescence correlation spectroscopy an

125 We use fluorescence recovery after photobleaching, fluorescence correlation spectroscopy, a

126 tion spectroscopy to quantify the diffusion, photobleaching, fluorescence intermittency, and photocon

127 agellar transport particles, or reduction of photobleaching for live microtubule imaging.

128 vo validation of interactions using acceptor photobleaching Forster resonance energy transfer and flu

129 wledge, no other fluorescence recovery after photobleaching framework incorporates all these model fe

130 Moreover, fluorescence recovery after photobleaching (FRAP) analysis demonstrated that exposur

131 ealed ghosts and fluorescence recovery after photobleaching (FRAP) analysis of actin filament mobilit

132 s we show, using fluorescence recovery after photobleaching (FRAP) and fluorescence anisotropy measur

133 -PEO) film using fluorescence recovery after photobleaching (FRAP) and single-molecule tracking (SMT)

134 Using fluorescence recovery after photobleaching (FRAP) and single-molecule tracking in hu

135 oscopy (FCS) and fluorescence recovery after photobleaching (FRAP) are widely used methods to determi

136 Fluorescence recovery after photobleaching (FRAP) assays revealed that the GFP-MuMx1

137 opy imaging, and fluorescence recovery after photobleaching (FRAP) assays, we show that the divalent

138 experiments with fluorescence recovery after photobleaching (FRAP) confirmed the active vesicle traff

139 Fluorescence recovery after photobleaching (FRAP) experiments confirmed the differen

140 method based on fluorescence recovery after photobleaching (FRAP) for determining how many reaction

141 lopment in 1976, fluorescence recovery after photobleaching (FRAP) has been one of the most popular t

142 es by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-t

143 Fluorescence recovery after photobleaching (FRAP) is a well-established experimental

144 Fluorescence recovery after photobleaching (FRAP) is an excellent tool to measure th

145 Fluorescence recovery after photobleaching (FRAP) is widely used to assess condensat

146 scent probe from Fluorescence Recovery After Photobleaching (FRAP) measurements assumes bleaching wit

147 Fluorescence recovery after photobleaching (FRAP) microscopy is used to probe the di

148 and the standard fluorescence recovery after photobleaching (FRAP) model introduced by Axelrod et al.

149 emonstrated using fluorescent recovery after photobleaching (FRAP) monitoring displacement of GFP-BAZ

150 as studied using fluorescence recovery after photobleaching (FRAP) of GFP-Galphas.

151 and using a dual fluorescence recovery after photobleaching (FRAP) reporter assay for axonal translat

152 Interestingly, fluorescence recovery after photobleaching (FRAP) results indicated that NKKY101 mut

153 Notably, fluorescence recovery after photobleaching (FRAP) shows that YscQ exchanges between

154 pid assessments, fluorescence recovery after photobleaching (FRAP) static laser microscopy, and deter

155 Fluorescence recovery after photobleaching (FRAP) studies indicate that like H1, bin

156 cribe the use of fluorescence recovery after photobleaching (FRAP) to probe chain mobility in reversi

157 oscopy (HS-AFM), fluorescence recovery after photobleaching (FRAP), confocal laser scanning microscop

158 chniques such as fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectros

159 We performed fluorescent recovery after photobleaching (FRAP), quantitative RT-PCR, and whole ce

160 Using fluorescence recovery after photobleaching (FRAP), we demonstrate that adherens junc

161 application, and Fluorescence Recovery After Photobleaching (FRAP).

162 established but underutilized method called photobleaching FRET (pbFRET), with the major difference

163 ourier transform fluorescence recovery after photobleaching (FT-FRAP) with patterned illumination is

164 Perturbation of equilibrium distributions by photobleaching has also been developed into a robust met

165 Single-molecule photobleaching has emerged as a powerful non-invasive ap

166 imaging can partially overcome the limits of photobleaching; however, limitations of this technique r

167 ncluding inverse fluorescence recovery after photobleaching (iFRAP) and photoactivatable probes, coup

168 Fluorescence recovery after photobleaching imaging reveals that the S561A mutant sho

169 e complex can be revealed by single-molecule photobleaching imaging.

170 Photoconversion, photobleaching, immunofluorescence and super-resolution

171 uorescent nanodiamonds (FNDs) show almost no photobleaching in a physiological environment.

172 scent signals in the presence of fluorophore photobleaching in a solid surface bioassay.

173 butable to the absence of phototoxicity, and photobleaching in bioluminescent imaging, combined with

174 been considered an effective means to reduce photobleaching in fluorescence microscopy, but a careful

175 spectroscopy and fluorescence recovery after photobleaching in HeLa cells.

176 stress by using fluorescence recovery after photobleaching in proplatelets with fluorescence-tagged

177 s and studies of fluorescence recovery after photobleaching in respiratory mucus showed that mechanis

178 Fluorescent recovery after photobleaching in slices from VGLUT1(Venus) knock-in mic

179 sterol probe, is combined with resistance to photobleaching in solution and in human fibroblasts and

180 Our results therefore indicate that photobleaching is a necessary step toward inflicting irr

181 Single molecule photobleaching is a powerful technique to measure the nu

182 theoretically that speckle imprinting using photobleaching is optimal when the laser energy and fluo

183 We confirm that STED Photobleaching is primarily caused by the depletion ligh

184 dard for experiments in which recovery after photobleaching is used to measure lateral diffusion.

185 labels (i.e., maximum emitted photons before photobleaching) is a critical requirement for achieving

186 ticles in a region of interest by repeatedly photobleaching its boundary.

187 tachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons t

188 ameters were optimized to deliver 23.8 mJ of photobleaching light energy at a pulse width of 6 msec a

189 Photobleaching limits extended imaging of fluorescent bi

190 st, we visualized whole eisosomes and, after photobleaching, localized recruitment of new Pil1p molec

191 s in cancer phototherapy is often limited by photobleaching, low tumor selectivity, and tumor hypoxia

192 In addition, their strong resistance to photobleaching makes them suitable for long duration or

193 Fluorescence recovery after photobleaching measurements revealed that single mismatc

194 A detailed investigation of the photobleaching mechanism of these porphyrazines revealed

195 ne by using FRET microscopy and the acceptor photobleaching method.

196 cted by dye concentration, light scattering, photobleaching, micro-viscosity, temperature, or the mai

197 Using fluorescence recovery after photobleaching microscopy, we show that apparent speed o

198 vant information that may be acquired before photobleaching occurs.

199 ed probe DNA on these surfaces is unlabeled, photobleaching of a probe label is not an issue, allowin

200 t absorbing, highlighting the possibility of photobleaching of BrC during their atmospheric aging and

201 The photobleaching of chromophoric dissolved organic matter

202 sensitive cells with no phototoxicity and no photobleaching of fluorescent biomarkers.

203 The system utilizes photobleaching of fluorophore dyes in the bulk flow and

204 ic receptor (M2R) and Gi1 by single-particle photobleaching of immobilized complexes.

205 en incorporated into phospholipid membranes, photobleaching of MgPzs correlates with the degree of li

206 ong-term detection is partially prevented by photobleaching of organic fluorescent probes.

207 umbers of molecules from fluctuations in the photobleaching of proteins tagged with Green Fluorescent

208 In the other approach, fractional photobleaching of reference oligomers provides a novel p

209 ation, the fundamental molecular event after photobleaching of rhodopsin is the recombination reactio

210 hat PKM2 phosphorylation is signaled through photobleaching of rhodopsin.

211 s technique remain present such as the rapid photobleaching of several types of organic fluorophores

212 Stepwise photobleaching of SpoIVFB fused to a fluorescent protein

213 Fluorescence recovery after photobleaching of tagged Nexus-associated proteins showe

214 We demonstrate that after photobleaching, (*)OH exposure degrades CDs in a two-ste

215 scopy as well as fluorescence recovery after photobleaching or photoswitching, and observed significa

216 y of an optical probe without suffering from photobleaching or phototoxicity.

217 via competitive absorption, and as a result, photobleaching or side reactions of the fluorophore are

218 l neurons, using fluorescence recovery after photobleaching, particle tracking, and modeling.

219 nalysis of multiple spatial harmonics of the photobleaching pattern.

220 The photobleaching patterns of eGFP-M2R were indicative of a

221 tical properties to fluoresce with near-zero photobleaching, photoblinking and background autofluores

222 rastructure, and fluorescence recovery after photobleaching/photoconversion experiments showed that t

223 However, photobleaching, phototoxicity, and related artifacts con

224 ted polymer to avoid leakage or differential photobleaching problems existed in other nanoprobes.

225 aging, including fluorescence recovery after photobleaching, provided further support for the role of

226 Single-molecule fluorescence recovery after photobleaching provides direct measurement of elongation

227 hotophysical parameters of the probe such as photobleaching quantum yield, count rate per molecule, a

228 inetic model to determine photoreduction and photobleaching rate constants.

229 In this paper, we show that the photobleaching rate in STED microscopy can be slowed dow

230 uorescence signal, photoconversion rate, and photobleaching rate of mEos3.2 sensitive to the buffer c

231 tended times is challenging due to the rapid photobleaching rate of most fluorophores.

232 tive redox reactions that contributed to the photobleaching rate were studied over a wide temperature

233 amples and provide constraints when modeling photobleaching rates in natural waters.

234 coherent and optimized for modeling accurate photobleaching rates in natural waters.

235 ntegrated signal and the photoconversion and photobleaching rates of fluorescent proteins in cells.

236 ter simulations, fluorescence-recovery-after-photobleaching recovery times of both fused and single-m

237 her increasing the linear scanning speed for photobleaching reduction in STED microscopy.

238 Photobleaching remains a limiting factor in superresolut

239 ion coefficient, low quantum yield, and high photobleaching resistance.

240 tremely low luminescence background and high photobleaching resistance.

241 Fluorescence recovery after photobleaching revealed that the viscosity of CF ASL was

242 Fluorescence recovery after photobleaching reveals reversible changes in the level o

243 s include mechanical uncertainties, specimen photobleaching, segmentation, and stitching inaccuracies

244 xperiments using fluorescence recovery after photobleaching show that human FZD4 assembles-in a DVL-i

245 ealed that the GFP-MuMx1 nuclear bodies upon photobleaching showed a slow partial recovery (mobile fr

246 Stepwise photobleaching showed that CaMKII formed oligomeric comp

247 The analysis of fluorescence recovery after photobleaching showed that the fluxes of dye molecules i

248 Fluorescence recovery after photobleaching shows that TSSC1 is required for efficien

249 ingle melanosome fluorescence recovery after photobleaching (smFRAP) to characterize the association

250 gy to perform single-molecule recovery after photobleaching (SRAP) within dense macromolecular assemb

251 ty traces over time is the quantification of photobleaching step counts.

252 sian analysis of images collected during the photobleaching step of each plane enabled lateral superr

253 Although photobleaching steps are often detected by eye, this met

254 Our method is capable of detecting >/=50 photobleaching steps even for signal-to-noise ratios as

255 orithms, it is possible to reliably identify photobleaching steps for up to 20-30 fluorophores and si

256 ties have created a challenge in identifying photobleaching steps in a time trace.

257 factors can limit and bias the detection of photobleaching steps, including noise, high numbers of f

258 While fluorescence recovery after photobleaching studies demonstrated that ROP dynamics do

259 ta receptor with fluorescence recovery after photobleaching studies on the lateral diffusion of a coe

260 Fluorescence recovery after photobleaching studies showed that these RTNLBs are mobi

261 Through photobleaching studies, we show that BMs are not static-

262 linked Orai1 concatemers and single-molecule photobleaching suggest that channels assemble as tetrame

263 A-wave recovery compared with WT mice after photobleaching, suggesting a delayed dark adaptation.

264 Using fluorescence recovery after photobleaching technique, diffusion coefficients D of fl

265 her, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral be

266 ters are frequently degraded by blinking and photobleaching that arise from poorly passivated host cr

267 CDOM origin (terrestrial versus marine) and photobleaching that controls variations in AQYs, with a

268 crease of fluorescence anisotropy seen after photobleaching the candidates.

269 es), we found that the higher the rate of PS photobleaching the faster the leakage induced in the mem

270 excitation zone by sequentially imaging and photobleaching the fluorescent molecules.

271 w blinking characteristics due to reversible photobleaching, the blinking of GNPs seems to be stable

272 After photobleaching, the EB1 signal at the flagellar tip reco

273 Irradiation with laser light above the photobleaching threshold induces photonic confinement po

274 icroscope enabled patterned illumination for photobleaching through two-photon excitation.

275 The noise properties in a photobleaching time trace depend on the number of active

276 t a new Bayesian method of counting steps in photobleaching time traces that takes into account stoch

277 Here, we applied single-molecule photobleaching to analyze the oligomeric state of an end

278 a procedure for fluorescence recovery after photobleaching to examine dye leakage through bacterial

279 GJs by applying fluorescence recovery after photobleaching to GJs formed from connexins fused with f

280 the anthropogenic source shows a shift from photobleaching to photohumification denoted by an increa

281 we use fluorophore localization imaging with photobleaching to probe the structure of EGFR oligomers.

282 including using chemical cleavage instead of photobleaching to remove fluorescent signals between con

283 st successful application of single-molecule photobleaching to resolve drug-induced and domain-depend

284 g a three-state model of photoconversion and photobleaching to the time course of fluorescence signal

285 uch as strong signal strength, resistance to photobleaching, tunable fluorescence emissions, high sen

286 r, near-membrane fluorescence recovery after photobleaching, uncaging or photoactivation/switching as

287 Importantly, CyB suffers photobleaching under a Nd:YAG laser but the signal decre

288 The technique of Fluorescence Recovery After Photobleaching was applied for the first time on real ch

289 ured by 2-photon fluorescence recovery after photobleaching, was not affected just after cardiorespir

290 rgy transfer and fluorescence recovery after photobleaching, we demonstrate that arrestin-3 dissociat

291 plete recovery of dextran fluorescence after photobleaching, we demonstrated that the actin ring-asso

292 Through live-cell photobleaching, we find rapid binding kinetics between P

293 Using fluorescence loss in photobleaching, we find that the endoplasmic reticulum (

294 urthermore using fluorescence recovery after photobleaching, we found that FAK inhibition increased t

295 studying the recovery of fluorescence after photobleaching, we found that there are three mantATP bi

296 Using fluorescence recovery after photobleaching, we show that an ACTN4 mutation that caus

297 Using fluorescence recovery after photobleaching, we show that the ER chaperone Kar2/BiP f

298 or wood smoke BrC, both photoenhancement and photobleaching were observed.

299 physical problems of acceptors such as rapid photobleaching, which is crucial for high time resolutio

300 per molecule, the saturation intensity, the photobleaching yield, and, crucially, management of brig