ライフサイエンスコーパス: photolabeling

1 Rs) were studied using electrophysiology and photolabeling.

2 and indinavir effectively protected against photolabeling.

3 econds after mixing, by use of time-resolved photolabeling.

4 , and top-down MS confirmed a single site of photolabeling.

5 imidodiphosphate afforded protection against photolabeling.

6 zyme, consistent with a 1:1 stoichiometry of photolabeling.

7 domains of microsomal PGHS-1 are subject to photolabeling.

8 ulator, which neither enhanced nor inhibited photolabeling.

9 roactive steroids inhibited etomidate analog photolabeling.

10 istance (CQR) affect the efficiency of AzBCQ photolabeling.

11 effect of phencyclidine (PCP) on [(125)I]TID photolabeling.

12 cid residues of the receptor for [125I]IACoc photolabeling.

13 Agonist enhanced the photolabeling 10-fold in a fragment containing the M1, M

14 Importantly, we did not detect significant photolabeling after deleting amino acid regions in Vps33

15 a site at the gamma-alpha subunit interface, photolabeling alphaM2-10 (alphaSer-252) and gammaMet-295

16 te); and (iii) at the gamma-alpha interface, photolabeling alphaM2-10'.

17 t sites within the alpha and delta subunits, photolabeling alphaVal-218 (alphaM1), deltaPhe-232 (delt

18 nds at the extracellular end of the channel, photolabeling amino acids at positions M2-16 (alpha,gamm

19 n gammaM3, and to a site in the ion channel, photolabeling amino acids within each subunit M2 helix t

20 he pivotal reactive intermediate involved in photolabeling and cross-linking studies using the 8-azid

21 gA in DMPC bilayers, direct [(14)C]halothane photolabeling and microsequencing demonstrated dominant

22 or and hence should be promising ligands for photolabeling and subsequent sequencing studies.

23 By photolabeling and transcriptionally profiling beta cells

24 oflurane binding sites were identified using photolabeling and were further validated by the docking

25 hR binding moiety, a benzophenone moiety for photolabeling, and an alkyne moiety for biotinylation vi

26 differential scanning calorimetry and lipid photolabeling, and measured the affinity of this interac

27 ,1'-binaphthyl-5,5'-disulfonic acid (BisANS) photolabeling approach to monitor changes in protein unf

28 In this study, we adapted a UV photolabeling approach, using an apolar fluorescent prob

29 nd subunit selectivity of [(3)H]azietomidate photolabeling are discussed in terms of the structures o

30 pharmacological specificity of nAChR subunit photolabeling as well as its dependence on [(3)H]tetraca

31 ids enhance rather than inhibit azietomidate photolabeling, as assayed at the level of GABA(A)R subun

32 A photolabeling assay showed that this annexin could bind

33 ATPase and photolabeling assays demonstrated that compounds with ri

34 Propofol enhanced [(3)H]AziPm photolabeling at alphaM2-10'.

35 The photolabeling at the cytoplasmic end of the channel is f

36 fen, whereas neither drug inhibits [(3)H]CPZ photolabeling at the extracellular end, establishing tha

37 Although the efficiency of photolabeling at the subunit level was unaffected by ago

38 de, but not an adenine nucleotide, following photolabeling, but prior to cross-linking.

39 acetyl-geranylgeranyl cysteine enhanced E193 photolabeling by 3-azibutanol.

40 how reserpine- and tetrabenazine-protectable photolabeling by [125I]IAmF.

41 mer-causing mutation in PS1 strongly reduced photolabeling by a transition-state analogue but not by

42 ncentration dependence of inhibition of that photolabeling by etomidate or R-mTFD-MPAB also establish

43 tates by using electrophysiology-coordinated photolabeling by several lipophilic probes followed by m

44 Photolabeling, by exciting the fluorescent drug-tubulin

45 Our visible light photolabeling can generate photocaged proteins for subse

46 These photolabeling data suggest that an accessory component w

47 (3)) resulted in complete protection against photolabeling, demonstrating that [(32)P]pApAp(8-azidoA)

48 had normal ATPase activity, indicating that photolabeling did not significantly alter the enzymatic

49 mately 0.5 mol of (14)C/mol of subunit, with photolabeling distributed within the nAChR extracellular

50 Using a combination of anesthetic photolabeling, electrophysiology, and molecular dynamics

51 species emit fluorescence at 650 nm enabling photolabeling entirely performed in the near-infrared ra

52 Competition photolabeling established that this site binds with high

53 l-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants o

54 Model photolabeling experiments and biological studies showed

55 ion kinetics, photoinactivation studies, and photolabeling experiments are also included; these exper

56 Photolabeling experiments have been particularly informa

57 Competitive radioligand binding and photolabeling experiments using well-characterized nonco

58 Photolabeling experiments with 8-azido-ATP demonstrate a

59 Photolabeling experiments with the McbA propeptide now i

60 Inhibition kinetics, photolabeling experiments, as well as X-ray crystallogra

61 he lipid carbonyl carbons, in agreement with photolabeling experiments.

62 o the fusion proteins were obtained from the photolabeling experiments.

63 roteins, including BamA and LptD as shown by photolabeling experiments.

64 (d) approximately 10 nM) in both binding and photolabeling experiments.

65 -myeloid differentiation-2 (MD-2) complex by photolabeling experiments.

66 analogue photolabeling reagents in which the photolabeling groups were placed at three positions arou

67 rescent groups for the purpose of performing photolabeling have been prepared and evaluated using the

68 IC50 = 40 mum) than it inhibited ion channel photolabeling (IC50 = 125 mum).

69 Photolabeling identified three distinct [(3)H]F(4)N(3)Bz

70 orms of RNase L has been completed utilizing photolabeling/immunoprecipitation and affinity assays, r

71 e alphaM1 and alphaM4 helices, identified by photolabeling in alphaM1 (alphaCys-222/alphaLeu-223); an

72 Intensity of photolabeling in each of the fractions examined coincide

73 Comparison of nAChR photolabeling in the closed state (absence of agonist) a

74 The propofol-inhibitable [(3)H]AziPm photolabeling in the GABAAR beta3 subunit in conjunction

75 Photolabeling in the ion channel and alphaM1 was higher

76 domain: 1) in the ion channel, identified by photolabeling in the M2 helices of betaVal-261 and delta

77 unit site in the delta subunit helix bundle, photolabeling in the nAChR desensitized state (+agonist)

78 a site within the ion channel, identified by photolabeling in the nAChR desensitized state of amino a

79 and deltaCys-236); (ii) in the ion channel, photolabeling in the nAChR resting, closed channel state

80 -55 and deltaTrp-57, as the primary sites of photolabeling in the non-alpha subunits.

81 presence of agonist and the agonist-enhanced photolabeling inhibitable by phencyclidine.

82 ne concentration establish that the observed photolabeling is at the high-affinity [(3)H]tetracaine-b

83 tubulin by subtilisin after, but not before, photolabeling is blocked by probe 1.

84 Aside from the high selectivity, the photolabeling is blue light-driven by a photoinduced ele

85 The observed pattern of photolabeling is examined in relation to the predicted o

86 [(3)H]CPZ photolabeling is not detected in the transmembrane domai

87 Photolabeling may help resolve this difficulty, and thus

88 Previous photolabeling, modeling, and functional data have identi

89 ce of 20 mM glutathione, indicating that the photolabeling observed for PGHS-1 was not due to the pre

90 n of this protein with CNBr/trypsin revealed photolabeling of a 2.9-kDa peptide.

91 imulated ABCB1 ATPase activity and inhibited photolabeling of ABCB1 with [(125)I]-iodoarylazidoprazos

92 erestingly, erlotinib slightly inhibited the photolabeling of ABCB1 with [(125)I]iodoarylazidoprazosi

93 imulated ABCG2 ATPase activity and inhibited photolabeling of ABCG2 with [(125)I]-IAAP.

94 ed as antihypertensive agents, inhibited the photolabeling of ABCG2 with [(125)I]IAAP and [(3)H]azido

95 gh concentration, but it did not inhibit the photolabeling of ABCG2 with IAAP.

96 at propofol inhibited to the same extent the photolabeling of alpha1Met-236 and betaMet-286.

97 However, it is unknown whether photolabeling of alphaE262 causes functional effects in

98 Irradiation at 254 nm resulted in photolabeling of alphaTyr(198) in agonist binding site S

99 CMPI-photolabeled nAChR subunits established photolabeling of amino acids contributing to the ACh bin

100 crosequencing, we found propofol-inhibitable photolabeling of amino acids in the beta3-alpha1 subunit

101 ing state), there was tetracaine-inhibitable photolabeling of amino acids in the ion channel at posit

102 ncing established 3alpha5alpha-P inhibitable photolabeling of amino acids near the cytoplasmic end of

103 The results of photolabeling of ArsA with the ATP analogue 8-azidoadeno

104 contrast, within the same site GABA enhances photolabeling of beta3Met-227 in betaM1 by an anesthetic

105 [(3)H]Azietomidate and [(3)H]R-mTFD-MPAB photolabeling of beta3Met-227 in betaM1 established that

106 re was also propofol-inhibitable [(3)H]AziPm photolabeling of beta3Met-227 in betaM1, the amino acid

107 [(3)H]azietomidate photolabeling of beta3Met-286 in betaM3 and alpha4Met-26

108 mical modification of this residue abolishes photolabeling of both channels with the ceramide probe.

109 05 in the vestibule of the ion channel, with photolabeling of both residues enhanced in the presence

110 fic, AMP-PCP-enhanced, [(3)H]azidodantrolene photolabeling of both the RyR monomer and a 160 or 172 k

111 Here, we use [(3)H]azietomidate photolabeling of bovine brain GABA(A)Rs to determine whe

112 imulated GLUT4 translocation, as assessed by photolabeling of cell surface GLUT4 with Bio-LC-ATB-BMPA

113 ition of agonist did not enhance [(125)I]TID photolabeling of deltaIle288 within the deltaM2-M3 loop.

114 Halothane photolabeling of deltaTyr-228 provides initial evidence

115 Within M1, the level of photolabeling of deltaTyr-228 with [(14)C]halothane was

116 Photolabeling of DnaA protein occurred with membrane pro

117 [35S]GTPgammaS binding, a decrease in basal photolabeling of G-proteins with azidoanilido-[alpha-32P

118 We previously identified azietomidate photolabeling of GABA(A)R alpha1Met-236 and betaMet-286

119 (A)R-modulating neurosteroids do not inhibit photolabeling of GABA(A)R alpha1Met-236 or betaMet-286 b

120 ) (GTPgammaS) binding and GTP hydrolysis and photolabeling of Galpha, we demonstrate highly efficient

121 Importantly, photolabeling of Galpha-subunits with azidoanilido-[alph

122 GABA inhibits S-[(3)H]mTFD-MPPB photolabeling of gamma2Ser-280 (gammaM2-15') in this sit

123 gamma-alpha subunit interface, identified by photolabeling of gammaMet299 within the gammaM3 helix at

124 Furthermore, [(3)H]physostigmine photolabeling of gammaTyr-111, gammaTyr-117, deltaTyr-21

125 Calmodulin antagonists increased photolabeling of hair-bundle myosin I by nucleotides.

126 ty labeling (BEProFL) approach that utilizes photolabeling of HDAC8 with a probe containing a UV-acti

127 Photolabeling of IDH with both [32P]2N3NAD+ and [2'-32P]

128 Photolabeling of intracellular molecules is an invaluabl

129 al analyses, radioligand binding assays, and photolabeling of nAChR-rich membranes with [3H]BP to ide

130 Compound 28 inhibited the photolabeling of P-gp with [(125)I]-iodoarylazidoprazosi

131 P produced time- and concentration-dependent photolabeling of protein bands of approximately 35 and 6

132 [125I]IAmF photolabeling of recombinant VMAT2, expressed in SH-SY5Y

133 hyperspectral visual stimulation as well as photolabeling of RGCs to provide a functional and anatom

134 specifically inhibits [(3)H]azidodantrolene photolabeling of RyR1 and its N-terminal fragment in SR.

135 Saturation of photolabeling of the 80- and the 37-kDa RNase L with the

136 8-Azidoadenosine 5'-monophosphate photolabeling of the AMP-binding site and adenylate kina

137 Photolabeling of the beta3 subunits was stereoselective,

138 Specific photolabeling of the Ca2+ ATPase with ZTG was obtained w

139 ATPase assays and photolabeling of the gpA K497A and gpA K497D mutant term

140 s not have an effect on the Kd value; and 3) photolabeling of the protein with a cysteine residue in

141 ([(125)I]TID) to compare the state-dependent photolabeling of the Torpedo nAChR before and after puri

142 The photolabeling of these amino acids suggests that when th

143 ersubunit sites, inhibited [(3)H]S-mTFD-MPPB photolabeling of these nAChR intrasubunit binding sites.

144 The photolabeling of this 80-kDa protein was saturable with

145 We now find that photolabeling of this pocket persists during the transit

146 Photolabeling of this protein by IAC was inhibited by SK

147 Photolabeling of this region indicates that the thymine

148 ein site, and propofol inhibited [(3)H]AziPm photolabeling of this site in myelin SIRT2.

149 solated from proteolytic digests established photolabeling of two residues: one within the alphaM1 tr

150 Preferential photolabeling on Pbeta from Pgamma position 40 and on Pa

151 SP prevented photolabeling only at concentrations higher than expecte

152 To achieve photolabeling or photoligation of two substrates, one is

153 esthetic steroid alphaxalone, which enhanced photolabeling, or DS-2, a delta subunit-selective positi

154 assays of the ATPase activity of P-gp and by photolabeling P-gp with its transport substrate [(125)I]

155 native mass spectrometry data, coupled with photolabeling performed in the presence of zinc, indicat

156 ytoplasmic end of the M2 ion channel domain, photolabeling positions M2-2, M2-6, and/or M2-9 in each

157 coupled to an alkyne-containing neurosteroid photolabeling reagent and used to identify peptide-stero

158 inding sites directly, a neurosteroid-analog photolabeling reagent, (3alpha,5beta)-6-azi-pregnanolone

159 4 was found to be an exceptionally efficient photolabeling reagent, incorporating into both alpha1 an

160 We synthesized neurosteroid analogue photolabeling reagents in which the photolabeling groups

161 ltaM2-13') that line the channel lumen (with photolabeling reduced by >90% in the desensitized state)

162 Intramembranous photolabeling shows that (i) protonation of TNF promotes

163 the feasibility of identifying neurosteroid photolabeling sites by using mass spectrometry.

164 ept of a cytocompatible and highly selective photolabeling strategy using a tryptophan-specific Ru-TA

165 Lastly, the photoprobe was also effective at photolabeling Streptococcus pyogenes hyaluronate synthas

166 emerging from structural, physiological and photolabeling studies as to where positive modulators bi

167 19-[3H]BPC-discodermolide), was selected for photolabeling studies because it had the highest extent

168 Photolabeling studies established that S-mTFD-MPPB binds

169 This was supported by photolabeling studies showing concentration- and UV-depe

170 Photolabeling studies using [3H]-3-azioctanol in Torpedo

171 Previous photolabeling studies with [(3)H]flunitrazepam identifie

172 DsRed-derived variants which we showcase in photolabeling studies, and discuss these data in terms o

173 Upon the basis of these photolabeling studies, we conclude that (1) subunits VII

174 he binding of ginkgolides to PAF receptor by photolabeling studies.

175 vative, [3H]RU58487, was used in binding and photolabeling studies.

176 rophenyl azido (pfpa) CQ analogues for PfCRT photolabeling studies.

177 In this study, we developed an intact cell photolabeling technique that allows the direct visualiza

178 (D-mannose-4-yloxy)-2-propylamine exofacial photolabeling technique, was reduced by approximately 70

179 s(D-mannos-4-yloxy)-2-propyl amine exofacial photolabeling technique.

180 Using photolabeling techniques, we show that mesenchymal cells

181 The photolabeling technology developed here offers a new way

182 Torpedo californica nAChRs and time-resolved photolabeling to identify the nAChR binding sites occupi

183 candidate targets, we used a combination of photolabeling, two-dimensional gel electrophoresis, and

184 xing unit, a novel freeze-quench unit, and a photolabeling unit.

185 This was confirmed by hydrophobic photolabeling using liposomes containing trace amounts o

186 on the full ectodomain LFA-1 were probed by photolabeling using photoactivatable isoflurane (azi-iso

187 purified recombinant PfCRT, we analyze AzBCQ photolabeling versus competition with CQ and other drugs

188 gh photoinsertion yield (approximately 30%); photolabeling was abolished in the presence of excess un

189 Within alpha subunit, >/=95% of specific photolabeling was contained within a 20-kilodalton prote

190 Photolabeling was inhibited by anesthetic concentrations

191 [(32)P-5N(3)]NAADP photolabeling was irreversible in a high K(+) buffer, a

192 Predominant hydrophobic photolabeling was localized within a single region of Dn

193 in-stimulated increase in cell-surface GLUT4 photolabeling was nearly identical (approximately 3-fold

194 This pattern of photolabeling was not affected by the presence of 20 mM

195 Also, photolabeling was observed with [32P]8N3ATP and [32P]2N3

196 Photolabeling was performed after preincubation times of

197 e antagonist, or isoflurane, state-dependent photolabeling was seen in a delta subunit fragment begin

198 Using in situ photolabeling, we captured their progeny exiting the nic

199 inhibitory effects elicited by these MSAs on photolabeling were distinct for beta-tubulin from differ

200 Photolabeling with 3-(trifluoromethyl)-3-(m-[125I]iodoph

201 vesicles of defined composition and by using photolabeling with 3-trifluoromethyl-3-(m-[125I]iodophen

202 Conversely, AMP enhanced photolabeling with 8-N3-ATP at ATP-binding site 2.

203 We now use competition photolabeling with [(3)H]azietomidate and [(3)H]R-mTFD-M

204 shows greatly decreased activity, abolished photolabeling with [32P]8N3ATP, and no detectable autoin

205 e recruitment of GLUT4 assessed by exofacial photolabeling with [3H]-ATB bis-mannose was reduced by 5

206 For both mutant and wild-type proteins, photolabeling with [3H]azidopine and [125I]iodoarylazido

207 Photolabeling with [3H]RU58487 under these optimal bindi

208 Thus, the photolabeling with [beta-32P]5-azido-UDP-GlcA has identi

209 lipid binding site, we have used hydrophobic photolabeling with a photoactivatable phosphatidylcholin

210 between Ca2+ and Na+,K+-ATPases, as well as photolabeling with a TG azido derivative, suggest that t

211 ytochrome c oxidase (CcO) were identified by photolabeling with arylazido-cardiolipin analogues and d

212 ults reported in this study demonstrate that photolabeling with azidonucleotides can be used to ident

213 rs that combines attributes of high-contrast photolabeling with high-sensitivity Ca(2+) detection in

214 ain of the Torpedo nAChR using time-resolved photolabeling with the hydrophobic probe 3-(trifluoromet

215 and cell surface GLUT4 levels as assessed by photolabeling with the membrane-impermeant reagent 2-N-(

216 Propofol inhibited [(3)H]AziPm photolabeling within the delta subunit helix bundle at l

217 lower-efficiency, state-dependent [(3)H]CMPI photolabeling within the ion channel.

218 or the purified nAChR, the agonist-sensitive photolabeling within the M2 ion channel domain of positi