ライフサイエンスコーパス: pili

1 or the antiphagocytic effect attributable to pili.

2 (MOI) correlates with detachment of longer F-pili.

3 rt of substrates and/or extrusion of type IV pili.

4 w for infection of strains with glycosylated pili.

5 r, but required for formation of conjugative pili.

6 eased expression of mannose-sensitive type 1 pili.

7 occi that expressed adhesive RrgA-containing pili.

8 us stiffness and the location of adhesins on pili.

9 aculum than for Sulfolobus and Saccharolobus pili.

10 rom both the bacterial flagellum and type IV pili.

11 c bacterial pathogen that expresses type IVa pili.

12 d to vary greatly between flexible and stiff pili.

13 he absence of appendages such as flagella or pili.

14 yr3 mutant, which produces poorly conductive pili.

15 ns, suggesting that it was incorporated into pili.

16 to form filaments with dimensions similar to pili.

17 directional motive force comes from Type IV pili.

18 nding adhesin at the tip of bacterial type 1 pili.

19 lular photoreceptors and mediated by Type IV pili.

20 ce factors diphtheria toxin and the adhesive pili.

21 ses of ComGC, the major pilin subunit of Com pili.

22 conformational changes in stretched type IV pili.

23 on which specifies pyelonephritis-associated pili.

24 ng Clostridium difficile, to produce Type IV pili.

25 , CdiA-CT(536) import requires conjugative F pili.

26 es the presence of pilins, but not assembled pili.

27 ent among the phages specific for retractile pili.

28 relatively recently repurposed from type IV pili.

29 for PilA, the monomer that assembles into e-pili.

30 s colonies through the action of the type IV pili.

31 ely short PilA monomers that assemble into e-pili.

32 nstruction of microbial strains expressing e-pili.

33 nge electron exchange without the need for e-pili.

34 using the mechanical activity of its type IV pili, a major surface adhesin.

35 y a positive feedback that increases type IV pili activity, thereby promoting long-term surface attac

36 ence such as toxins, adhesins, flagella, and pili, among others.

37 s utilized by these bacteria are the type IV pili and a protein O-glycosylation system.

38 evels of shaft pilins and SrtC2 produce long pili and block coaggregation by SrtA(+) bacteria.

39 d to nutrient availability for production of pili and exopolysaccharide adhesion structures.

40 hesins (e.g., LecA and LecB lectins, type VI pili and flagella) and iron to invade host cells with th

41 which have surfaces decorated with discrete pili and form a dispersed layer of cells on a plastic su

42 We identified EmpA as the tip of the pili and found that deletion of empA reduced biofilm for

43 pilus biosynthesis, results in cells lacking pili and having an adhesion defect, it does not affect m

44 mechanism that is distinct from other known pili and likely represents a different type of bacterial

45 ynechocystis sp. PCC 6803 moves with Type IV pili and measures light intensity and color with a range

46 atio biological nanowires, such as bacterial pili and neurites, mediate many of the interactions and

47 s for intracellular coordination of multiple pili and of pili with other motility machines, ranging f

48 y applicable method for labeling and imaging pili and other surface-exposed nanomachines in live cell

49 tem that regulates the production of type IV pili and potentially other systems in certain gammaprote

50 d by the extension and retraction of type IV pili and requires the presence of exopolysaccharides (EP

51 emonstrate that G. sulfurreducens conductive pili and the outer membrane extensions of S. oneidensis

52 xococcus xanthus cells to visualize type IVa pili and the protein machine that assembles and retracts

53 NA), FasX, which regulates the expression of pili and the thrombolytic agent streptokinase.

54 eria associated properties such as number of pili and their distribution on the cell body and environ

55 The broad conservation of the type IV pili and their importance in pathogens for host coloniza

56 PilX require PilY1 for inclusion in surface pili and vice versa, suggestive of complex formation.

57 rial type 2 secretion systems (T2SS), type 4 pili, and archaeal flagella assemble fibres from initial

58 de up to 16 distinct chaperone-usher pathway pili, and each pilus type may enable colonization of a h

59 owed that DMBT1 bound Pseudomonas aeruginosa pili, and inhibited twitching motility, a pilus-mediated

60 , demonstrate its incorporation into Type IV pili, and offer insights into how the Type IV pili of C.

61 tance between the surface and polar adhesive pili, and orienting pili to face the surface.

62 n-pilin protein PilY1 for incorporation into pili, and that with FimU, PilE may couple the priming su

63 everal bioelectrochemical technologies and e-pili are a promising renewable source of electronic mate

64 observed conductive properties of Geobacter pili are a valuable tool to guide further investigation

65 monstrate a mechanism by which Gram-positive pili are able to dissipate mechanical energy through mec

66 nsion is a quasistatic process such that the pili are able to relax via thermal fluctuations as it is

67 Geobacter sulfurreducens pili are actual wires.

68 These pili are assembled by the conserved chaperone-usher (CU)

69 Pili are assembled in two distinct but coupled steps, an

70 These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholip

71 and structural analyses reveal that F17-like pili are closely related to pilus types carried by intes

72 posed of the major pilin PilA4, while narrow pili are composed of a so-far uncharacterized pilin whic

73 Wide pili are composed of the major pilin PilA4, while narrow

74 The Streptococcus pnuenomae pilus island 1 pili are composed of three subunits, RrgA, RrgB, and Rrg

75 utant cells (0.2 events/min), indicating the pili are critical structures in the transition from reve

76 hair-like cell appendages denoted as type IV pili are crucial for biofilm formation in diverse eubact

77 Thus, pili are dispensable for biofilm development in this cya

78 Pili are elongated structures that protrude from bacteri

79 The pili are essential for long-range electron transport to

80 Type IV pili are extracellular polymers of the major pilin subun

81 Pili are fibrous appendages expressed on the surface of

82 Type IVa pili are filamentous cell surface structures observed in

83 We show that the rupture forces between pili are fine-tuned by post-translational modification.

84 Type IV pili are flexible filaments on the surface of bacteria,

85 The genes encoding type IV pili are found universally in the Gram-negative, aerobic

86 Type IV pili are important virulence factors for many pathogens,

87 Type IV pili are important virulence factors on the surface of m

88 Ubiquitous microbial appendages called pili are involved in sensing surfaces and facilitating d

89 Conversely, stiff pili are much more likely to make contact with the subst

90 ria, extracellular protein appendages termed pili are necessary for adherence under mechanical stress

91 Pili are proteinaceous polymers of linked pilins that pr

92 Types 1 and P pili are prototypical bacterial cell-surface appendages

93 nvelope protein PilY1 and functional type IV pili are required mechanosensory elements.

94 Type IVa pili are ubiquitous and versatile bacterial cell surface

95 Conjugative pili are widespread bacterial appendages that play impor

96 The putative pili are, in fact, long extensions of the cytochrome-ric

97 in the DeltapilA[1-6]DeltaaglB cells, these pili are, unlike wild-type pili, curled, perhaps renderi

98 These "competence pili" are composed of the major pilin protein ComGC and

99 Fimbriae (also known as pili) are appendages that extend up to 2 mum beyond the

100 mation of outer-membrane vesicles (OMVs) and pili, as well as several other cell envelope alterations

101 When compared with PpdD pili assembled in a heterologous Klebsiella T2SS type 2

102 These pili, assembled by the chaperone-usher pathway, are poly

103 as groups of spherical particles and Type IV pili attached to bacteria are modelled as dynamic spring

104 f the ctpABCDEFGHI genes (cluster of type IV pili; Atu0216 to Atu0224), homologous to tad-type pilus

105 iety of virulence factors, including type IV pili, bacterial extracellular appendages often essential

106 ficiently move toward chemoattractants using pili-based "twitching" motility and the Chp chemosensory

107 nhibit twitching motility and showed reduced pili binding (~40%).

108 twitching motility (~57%), without reducing pili binding.

109 g motility involves its N-glycosylation, its pili-binding capacity is insufficient, and it cannot be

110 plastic surface, DeltaaglB cells have thick pili bundles and form microcolonies.

111 hat do not depend on traditional flagella or pili, but are powered by mechanisms that are less well u

112 ata indicate a role for flagellin and type I pili, but not the nuclease, S-layer protein, or serratam

113 ling a new pilin superfamily, assembled into pili by a distinct fifth pathway.

114 he high force extensibility, CnaA-containing pili can dissipate approximately 28-fold as much energy

115 Electrically conductive pili (e-pili) can be an important electrical conduit for DIET.

116 ping sortase SrtA generates exceedingly long pili, catalyzed by its pilus-specific sortase SrtC2 that

117 red for assembly of their respective Type IV pili, CFA/III and Longus.

118 owires were previously thought to be type IV pili composed of PilA protein.

119 atly diminished the abundance of PilA, and e-pili could no longer be detected.

120 aglB cells, these pili are, unlike wild-type pili, curled, perhaps rendering them non-functional.

121 tive pili remains uncertain, largely because pili-defective mutants also have cytochrome defects.

122 Here we report that a pili-deficient mutant carrying an inactivating mutation

123 otility, conditionally important for type IV pili-dependent motility and required to complete the dev

124 faces as structured swarms utilizing type IV pili-dependent social (S) motility.

125 It is shown that very flexible pili do not extend very far and thus would limit the bac

126 us-minus mutant cells (13 s), suggesting the pili do not play a significant role in reversible adhesi

127 Electrically conductive pili (e-pili) can be an important electrical conduit for

128 he expression of the electrically conductive pili (e-pili) of Geobacter species are of interest becau

129 lacking endocarditis- and biofilm-associated pili (Ebp) exhibited a decreased ability to associate wi

130 bacteria to short jumps forward while stiff pili enable much greater displacements.

131 cytosensor was tested for detection of F(+) pili Escherichia coli species, using XL1-Blue and K12 st

132 The pili extend, attach to the surface, and then retract to

133 In many bacteria and archaea, type IV pili facilitate surface adhesion, the initial step in bi

134 Upon retraction of pili filaments, the monomeric pilin reservoir in the inn

135 , DNABII (DNA binding and bending) proteins, pili, flagella, and outer membrane vesicles.

136 olved in iron acquisition (n = 67), fimbriae/pili/flagella production (n = 117), and metal homeostasi

137 d by surface structures such as flagella and pili, followed by a permanent adhesion stage usually med

138 surface-exposed, proteinaceous fibers called pili for diverse behaviors, including horizontal gene tr

139 ate that P. aeruginosa not only uses type IV pili for surface-specific twitching motility, but also a

140 e product in protein secretion as well as in pili formation.

141 entifying microorganisms likely to express e-pili from (meta)genomic data and for the construction of

142 Adhesive type 1 pili from enteroinvasive, Gram-negative bacteria mediate

143 NA phage infection triggers the release of F-pili from host cells, and that higher multiplicity of in

144 ross-links, formed autocatalytically, in the pili from Streptococcus pyogenes has highlighted the rol

145 e pneumococcus, the coordinated secretion of pili from the cells correlates to DNA transformation.

146 e main structural subunit of adhesive type 1 pili from uropathogenic Escherichia coli strains.

147 ases; a set of 20 genes required for type IV pili function; and several conditionally essential genes

148 odels of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstruct

149 lobal comparative analysis, we show that Com pili genes are virtually ubiquitous in Bacilli, a major

150 cal conductivity of Geobacter sulfurreducens pili has been documented with multiple lines of experime

151 Pili have been shown to play a role in the pathogenesis

152 hewanella oneidensis also produce conductive pili have recently been recanted, based on novel live-im

153 Physically blocking pili imposed resistance to pilus retraction, which was s

154 ebacterium diphtheriae and the heterodimeric pili in Actinomyces oris, highlighting some newly emerge

155 on the remarkable properties of conjugative pili in bacterial secretion and phage infection.

156 two prototypical models, the heterotrimeric pili in Corynebacterium diphtheriae and the heterodimeri

157 interest because of the important role of e-pili in diverse biogeochemical processes, anaerobic dige

158 olved in the formation of competence-induced pili in Gram-positive bacteria and corroborate the remar

159 Adhesive pili in Gram-positive bacteria represent a variety of ex

160 equired for pilus formation, and the role of pili in T4SS function is unclear.

161 Although PilA4 assembles into pili in the DeltapilA[1-6]DeltaaglB cells, these pili ar

162 g the dynamic activity of type IV competence pili in V. cholerae as a model system.

163 ently confirmed charge propagation along the pili, in a manner similar to carbon nanotubes.

164 ectin located at the tip of bacterial type 1 pili, interacts with mannosylated glycoproteins on the u

165 of the structurally related archaeal type IV pili is unknown.

166 study, the effects of A. muciniphila and its pili-like protein Amuc_1100 on macrophage polarization d

167 ew densely packed ribosomes and a variety of pili-like structures that might enable inter-organism in

168 a, and twitching motility powered by Type IV pili, little is known about gliding motility.

169 ogarithmic range of detection (i.e., 3-7 for pili-mannose binding and 2-8 for Con A mediated binding)

170 ce (QCM) transducers and by using the direct pili-mannose binding as well as Concanavalin A (Con A) m

171 stream behaviors, but the mechanism by which pili mediate surface sensing has been unclear.

172 Adhesive surface structures termed fimbriae (pili) mediate interactions of P. gingivalis with other b

173 rance from the bacterial surface and loss of pili-mediated functions.

174 Like other chemotaxis pathways, pili-mediated surface sensing results in a transient res

175 channel, in contrast to previously existing pili models.

176 d actions of the hair follicle, the arrector pili muscle (APM), and the sympathetic nerve, providing

177 regeneration of hair follicles with arrector pili muscles in healed wounds.

178 In the skin, sympathetic nerves, arrector pili muscles, and hair follicles form a tri-lineage unit

179 immune cells, sensory neurons, and arrector pili muscles.

180 ay but not the functioning of the conductive pili network.

181 d no activity and did not bind P. aeruginosa pili; nor did recombinant DMBT1 (aa 1-220) or another SR

182 ellular milieu (exo-proteome) and eliminated pili observable by electron microscopy.

183 ili, and offer insights into how the Type IV pili of C. difficile may assemble and function.

184 tite attached to the electrically conductive pili of Geobacter species in a manner reminiscent of the

185 e multi-heme c-type cytochrome OmcS with the pili of Geobacter sulfurreducens.

186 to the cell surface allows for production of pili of sufficient length to support adherence and motil

187 ereby compensating for the reduced number of pili of the N3 mutant.

188 PilA1 and PilA2, the most abundant pilins in pili of wild-type and DeltaaglB strains, are modified un

189 ssion of the electrically conductive pili (e-pili) of Geobacter species are of interest because of th

190 a plethora of colonization factors (fimbriae/pili), of which CFA/I and CFA/II, which are assembled vi

191 tase and the spatial positioning of adhesive pili on the cell surface modulated by the housekeeping s

192 Assembly of pili on the gram-positive bacterial cell wall involves 2

193 Pili on the surface of Sulfolobus islandicus are used fo

194 uce protein polymers on their surface called pili or fimbriae that serve either as attachment devices

195 assemble adhesive surface structures termed pili or fimbriae to initiate and sustain infection of ho

196 johnsoniae, a rod-shaped bacterium devoid of pili or flagella, glide over glass at speeds of 2-4 mum/

197 Movement control (pili or gliding) in other deltaproteobacteria, such as t

198 formation occurs in the absence of flagella, pili, or certain polysaccharides.

199 eriously on surfaces without using flagella, pili, or other external appendages.

200 ults support a model in which the conductive pili permeate the biofilms to wire the cells to the cond

201 pneumoniae expresses two different types of pili, PI-1 and PI-2, both of which require the concerted

202 Cell surface appendages called pili play an important role in adhesion and biofilm form

203 Pili, polymerized protein structures covalently anchored

204 their biofilm-promoting function in type IV pili-producing heterotrophic bacteria.

205 tween cyanobacteria and well-studied type IV pili-producing heterotrophic bacteria.

206 ouse model, we show that F17-like and type 1 pili promote intestinal colonization and show distinct b

207 t are functional components of flagellin and pili proteins within clinically relevant Gram-negative b

208 long-range charge transport along individual pili purified free of metal and redox organic cofactors

209 Genes encoding types I and IV pili, quorum sensing components, and proteins involved i

210 the contribution of the biofilm's conductive pili remains uncertain, largely because pili-defective m

211 Nevertheless, pili removal is not required for biofilm formation as ev

212 ary tract infections, expresses type 1 and P pili required for colonization of the bladder and kidney

213 enes revealed that the production of type IV pili required the presence of the Le2152 gene, which enc

214 ing cryo-electron microscopy (cryoEM), these pili showed indistinguishable helical parameters, emphas

215 f pilR2 resulted in a reduction of assembled pili, significant decreases in EPS production and loss o

216 We show that by changing pili substrate interactions, the motility pattern can be

217 ility is independent of flagella and type IV pili, suggesting a novel mechanism of cell migration in

218 PilA stability prior to incorporation into e-pili, suggesting that Spc has a chaperone function that

219 However, the mechanism by which the pili surrounding the cell body work together to propel b

220 nnose-sensitive hemagglutinin (MSHA) type IV pili synergistically to switch between two complementary

221 ing enabled us to identify a chaperone-usher pili system (Kpi) in Kp3380.

222 lfolobus cells and act downstream of the Ups pili system.

223 Type 1 pili (T1P) are major virulence factors for uropathogenic

224 n features, T4P are classified into type IVa pili (T4aP) and type IVb pili (T4bP)(1,2).

225 Type 4a pili (T4aP) are long, thin and dynamic fibres displayed

226 cluding Pseudomonas aeruginosa, use type IVa pili (T4aP) for attachment and twitching motility.

227 ified into type IVa pili (T4aP) and type IVb pili (T4bP)(1,2).

228 ndent on extension and retraction of Type-IV pili (T4P) and production of extracellular polysaccharid

229 stems and are linked to extrusion of type IV pili (T4P) and to DNA uptake.

230 Type IV pili (T4P) are among the key virulence factors used by P

231 Type IV pili (T4P) are bacterial appendages composed of protein

232 Bacterial type 4 pili (T4P) are extracellular polymers that initiate the

233 Type IV pili (T4P) are filamentous appendages found on many Bact

234 Bacterial type IV pili (T4P) are polymeric protein nanofibers that have di

235 Type IV pili (T4P) are ubiquitous and versatile bacterial cell s

236 Type IV pili (T4P) are ubiquitous bacterial cell surface structu

237 Type IV pili (T4P) are very thin protein filaments that extend f

238 on systems that regulate motility by type IV pili (T4P) can be markedly more complex than related fla

239 Type IV pili (T4P) contain hundreds of major subunits, but minor

240 Neisseria meningitidis use Type IV pili (T4P) to adhere to endothelial cells and breach the

241 , exquisitely thin appendages called type IV pili (T4P), dynamic filaments that are rapidly polymeriz

242 (cryo-EM) structures of two archaeal type IV pili (T4P), from Pyrobaculum arsenaticum and Saccharolob

243 teria can move across surfaces using type IV pili (T4P), which undergo cycles of extension, adhesion,

244 a exhibit surface motility powered by type 4 pili (T4P).

245 ransporter adhesins, O antigens, and type IV pili (T4P).

246 xins, or filamentous phages; extrude type IV pili (T4P); or take up DNA.

247 Type IV pili (Tfp) are functionally versatile filaments, widespr

248 Type IV pili (Tfp) are highly conserved macromolecular structure

249 and appendages known as flagella and type IV pili (TFP) are known to confer such motility.

250 the diverse world of bacterial pili, type IV pili (Tfp) are unique for two reasons: their multifuncti

251 Type IV pili (TFP) function as mechanosensors to trigger acute v

252 ough the extension and retraction of type IV pili (TFP) on solid surfaces, which requires both TFP an

253 orm of PilA [the majority subunit of Type IV pili (Tfp) produced by NTHI], mediated gradual 'top-down

254 cterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous ba

255 he ability to walk on surfaces using type IV pili (TFP), a motility mechanism known as twitching(1,2)

256 ve across surfaces by using multiple Type IV Pili (TFP), motorized appendages capable of force genera

257 Type IV pili (Tfp), which are key virulence factors in many bact

258 Type IV pili (Tfp), which have been studied extensively in a few

259 emerged as a model for the study of type IV pili (Tfp)-exceptionally widespread and important prokar

260 antifungal virulence factors is the type IV pili that are required for twitching motility.

261 coded by conjugative plasmids expressing sex-pili that can readily spread resistance through bacteria

262 dermal collagen, sweat glands, and arrector pili that engulfed axons.

263 While it is not always the tip of flexible pili that first makes contact with the substrate, it is

264 y observed metallic like conductivity of the pili that has been attributed to overlapping pi-pi orbit

265 substrate, it is likely to be a part of the pili that is close to the tip.

266 the resistance on retracting, surface-bound pili that occurs upon surface contact.

267 e bacterial cell wall or assemble fiber-like pili that promote bacterial adhesion.

268 toxins, RHS proteins, adhesins, and type IV pili] that likely mediate cell-cell interactions and gut

269 Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the

270 re, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron

271 ion of the metallic-like conductivity of the pili, their role in biogeochemical cycling, and applicat

272 hesion strength to the surface of individual pili, thereby increasing effective pulling time during r

273 ion mechanism requires attachment of type IV pili to a solid surface, followed by pilus retraction an

274 Thus, UPEC employ separate CUP pili to adapt to the rapidly changing niche during bladd

275 rface and polar adhesive pili, and orienting pili to face the surface.

276 pping as the mechanism that allows Geobacter pili to function as protein nanowires between the cell a

277 counter-rotates the cell body, causing MSHA pili to have periodic mechanical contact with the surfac

278 a such as Pseudomonas aeruginosa use type IV pili to move across surfaces.

279 of pilin subunits and another for anchoring pili to peptidoglycan.

280 xpression of conductive protein filaments or pili to respire extracellular electron acceptors such as

281 gen Streptococcus pneumoniae deploys type IV pili to take up DNA during transformation.

282 r, twitching-mode motility employs hair-like pili to transverse moist surfaces with a jittery irregul

283 rmal flattening and twisting of hair shafts (pili torti) and hearing problems.

284 , also known as "spun glass hair syndrome," "pili trianguli et canaliculi," or "cheveux incoiffables"

285 ment (TFF) superfamily, comprised of type IV pili, type II secretion systems (T2SSs), archaella, and

286 In the diverse world of bacterial pili, type IV pili (Tfp) are unique for two reasons: the

287 is known about the behavior of Gram-positive pili under force.

288 We visualized Caulobacter crescentus pili undergoing dynamic cycles of extension and retracti

289 zation of extracellular nanomachines such as pili using this approach can provide a more comprehensiv

290 to biofilm formation (BopD), adherence (Epb pili), virulence (cps loci, gelatinase, SprE) and antibi

291 retical energy-minimized models of Geobacter pili were constructed with a previously described approa

292 Surprises include that tight adherence (Tad) pili were horizontally acquired from Archaea and that T2

293 The pili, when removed from cells, resist digestion by tryps

294 es (likely to be outer membrane proteins and pili) which, upon contacting the membrane, undergo surfa

295 Movement depends on Type IV pili, which are extended, adhere to the substrate and th

296 UPEC express CUP pili, which are extracellular fibers tipped with adhesin

297 at a single point into individual, untreated pili, which are still attached to cells, propagated over

298 he crenarchaeal genus Sulfolobus express Ups pili, which initiate cell aggregate formation.

299 Results are generated for pili with different rigidities ranging from very flexibl

300 ellular coordination of multiple pili and of pili with other motility machines, ranging from physical