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1 y when it is tested against the real target (plasmid DNA).
2 wer for the attenuated DNA and close for the plasmid DNA.
3 asmid DNA and per 10(8) cells with a 13.8 kb plasmid DNA.
4 m minicircles was 5-10-fold higher than with plasmid DNA.
5 st defense by interfering with the uptake of plasmid DNA.
6 (HFFs) infected with HSV or transfected with plasmid DNA.
7 and influencing intracellular trafficking of plasmid DNA.
8 264.7 and DC 2.4 cells using GFP-expressing plasmid DNA.
9 omplexes with a high packaging efficiency of plasmid DNA.
10 idge between PEGylated anionic liposomes and plasmid DNA.
11 d ssDNA and stabilize negative supercoils in plasmid DNA.
12 to target PCR-derived linear double-stranded plasmid DNA.
13 immune responses following immunization with plasmid DNA.
14 key phosphorylation site involved in DNA-PK) plasmid DNA.
15 a novel eukaryotic alternative to bacterial plasmid DNA.
16 mited by detection of contaminating viral or plasmid DNA.
17 rocapsule position on cell transfection with plasmid DNA.
18 plexes formed using BFDMA and macromolecular plasmid DNA.
19 crA helicase to bind and begin unwinding the plasmid DNA.
20 lear membrane to advance nuclear delivery of plasmid DNA.
21 ed muscles over muscles treated with "naked" plasmid DNA.
22 nse than intramuscular injection of the same plasmid DNA.
23 and DNA nodes, within negatively supercoiled plasmid DNA.
24 in vivo and in human genomic DNA spiked with plasmid DNA.
25 iates at multiple single-stranded regions of plasmid DNA.
26 DNA preparations are contaminated with XMRV plasmid DNA.
27 cles (VLPs) when expressed individually from plasmid DNA.
28 tion of 12 novel DeltaG rabies variants from plasmid DNA.
29 rotinin that they nick supercoiled, circular plasmid DNA.
30 the faithful replication of chromosomal and plasmid DNA.
31 e local microbubble-enhanced sonoporation of plasmid DNA.
32 n health, often residing in extrachromosomal plasmid DNA.
33 ved 20% 5-gmC in the genome and 45% 5-gmC in plasmid DNA.
35 issue, we used intradermal immunization with plasmid DNA, a system in which activation of CD8(+) T ce
37 und that mice injected with 50mug luciferase plasmid DNA and 5x10(5) microbubbles followed by ultraso
39 caused restriction of incoming bacteriophage/plasmid DNA and endogenous chromosomal DNA within Escher
40 cationic polymers and fluorescently labeled plasmid DNA and injected them intradermally into mice.
41 to consistently enhance the delivery of both plasmid DNA and messenger RNA payloads in stem cells, pr
42 formation efficiency up to 10(7) CFU per mug plasmid DNA and per 10(8) cells with a 13.8 kb plasmid D
44 onfocal live cell imaging, we show that both plasmid DNA and siRNA are internalised via endocytosis.
46 homatis mouse pneumonitis (MoPn) genomic and plasmid DNA and tested it with serum samples from MoPn-i
47 Heterologous prime-boost immunization with plasmid DNA and viral vector vaccines is an emerging app
51 We transfected various source cells with plasmid DNAs and stimulated the cells with a focal and t
52 , were susceptible to the virus rescued from plasmid DNAs and supported production of the virus over
54 es were evaluated using combinations of both plasmid-DNA and transposase-protein relocalization to th
55 s, germline transgenesis, electroporation of plasmid DNA, and microinjection of morpholinos are all r
56 he NPC1(-/-) null mouse, and delivery of the plasmid DNA, and NPC1 mRNA expression in brain, spleen,
57 es not affect the biological activity of the plasmid DNA, and that the core-shell formulations have n
58 In mouse T cells, cGAS KO ablated sensing of plasmid DNA, and TREX1 KO enabled cells to sense short i
61 of redox control observed for lipoplexes of plasmid DNA are maintained in complexes formed using sma
62 cal assays based on Cre-mediated knotting of plasmid DNAs are consistent with a right-handed chiral j
65 , we tested a different formulation in which plasmid DNA associates with the surface of preformed 20-
66 human and canine cells partition transfected plasmid DNA asymmetrically, preferentially into the daug
68 necessary to orchestrate the propagation of plasmid DNA between bacterial cells through conjugative
69 with spiky surfaces demonstrate the highest plasmid DNA binding capability and transfection efficacy
70 , BALB/c mice were given three injections of plasmid DNA-Bla g 1 prior to sensitization with two prim
73 h cisplatin enhances double-strand breaks of plasmid DNA by a factor of approximately 3.5 and dramati
74 rt that (-)-lomaiviticin A nicks and cleaves plasmid DNA by a pathway that is independent of reactive
75 nwinding of both linear and natural circular plasmid DNA by PcrA/RepD was followed in real-time using
77 he type IV REase in restricting 5mC-modified plasmid DNA by transformation into clinical S. aureus st
78 4(+) T cells expressed cGAS and responded to plasmid DNA by upregulation of ISGs and release of bioac
79 We found that site-specific mutations in plasmid DNA can be generated in Escherichia coli using m
82 le-mediated transcutaneous immunization with plasmid DNA can potentially induce a stronger immune res
83 e screening, rolling circle amplification of plasmid DNA, capillary electrophoresis and automated dig
84 SB100X hyperactive transposase together with plasmid DNA carrying a transgene construct flanked by bi
85 eractive transposase, together with circular plasmid DNA carrying a transgene construct flanked by bi
87 Stable transfection of HEK-293T cells with plasmid DNA carrying EqCXCL16 (HEK-EqCXCL16 cells) incre
88 measurements of a model system of clustered plasmid-DNA centered by the dynamically unstable actin-l
89 AS-dependent cell-intrinsic response to both plasmid DNA challenge or inoculation with HSV-1DeltaUL41
92 licited a stronger immune response than with plasmid DNA-coated net negatively charged nanoparticles
95 rticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-ly
96 noparticles, composed of single molecules of plasmid DNA compacted with block copolymers of poly-l-ly
97 linked to itself interacts differently with plasmid DNA compared to conventional vectors and when te
98 hese data demonstrate that mucosally applied plasmid DNA complexed to PEI followed by a mucosal prote
99 Significant differences in behavior toward plasmid DNA condensation are correlated with biological
101 ional non-viral gene transfer uses bacterial plasmid DNA containing antibiotic resistance genes, cis-
102 say using mammalian cell nuclear extract and plasmid DNA containing bulky adducts formed by N-acetoxy
104 thin the procapsid and recircularizes linear plasmid DNA containing two terminal loxP recognition sit
106 cted nucleosome positioning in vitro on four plasmid DNAs containing DNA fragments derived from the g
107 ceptor-targeted, DNA-binding peptide (P) and plasmid DNA (D), which electrostatically self-assembled
110 When combined with amphipathic peptide-based plasmid DNA delivery, these bioinks supported enhanced n
112 iption-coupled hypernegative supercoiling of plasmid DNA did not need the expression of a membrane-in
115 demonstrate how high-speed AFM can visualize plasmid DNA dynamics, intermittent nucleosome-nucleosome
118 strate co-delivery of luciferase protein and plasmid DNA encoding a fluorescent protein from two diff
121 ing a natural phenomenon observed in tumors, plasmid DNA encoding for a soluble ligand to NKG2D (sRAE
122 ity and eTE were systematically explored for plasmid DNA encoding green fluorescent protein following
123 , we administered intracranial injections of plasmid DNA encoding IL-10 (pDNA-IL-10) into the NAc of
124 e prepared via freeze drying and loaded with plasmid DNA encoding perlecan domain I and VEGF189 and a
127 umor immunity induced upon immunization with plasmid DNA encoding SV40 Tag as a transgene (pCMV-Tag).
129 mia that hydrodynamic tail-vein injection of plasmid DNA encoding the adenine base editor (ABE) and a
132 we inoculated nonhuman primates (NHPs) with plasmid DNA encoding transmembrane-anchored, cleaved JRF
133 s suggest that chitosan scaffolds containing plasmid DNA encoding VEGF189 and perlecan domain I have
136 UvrC from Mtb collectively bound and cleaved plasmid DNA exposed to ultraviolet (UV) irradiation or p
137 d hydrodynamic injection to deliver a CRISPR plasmid DNA expressing Cas9 and single guide RNAs (sgRNA
138 li (E coli) containing plasmids, followed by plasmid DNA extraction and purification prior to downstr
139 uine IgG obtained from horses immunized with plasmid DNA followed by boosting with Kunjin replicon vi
140 ecifically evaluated the advantages of using plasmid DNA for sequencing and the value of supplementin
141 can be genetically programmed with nonviral plasmid DNA for the biogenesis and delivery of antisense
142 ation of parasites in a sample, we developed plasmid DNAs for all the three assay targets for absolut
143 We show that peptide efficiently packaged plasmid DNA forming spherical, highly cationic nanocompl
144 i, such as by insertion of foreign (viral or plasmid) DNA fragments into clustered regularly interspa
146 ve B. burgdorferi much more efficiently than plasmid DNA from E. coli, particularly when the bbe02 an
147 rBCP was also found to protect supercoiled plasmid DNA from oxidative damage (i.e., nicking) in vit
148 or CMV, or transient transfection with naked plasmid DNA, HIRA re-localizes to PML bodies, sites of c
149 sp and Imu1-3 and examined their activity on plasmid DNA, human umbilical vein endothelial cells, and
150 persistent transgene expression compared to plasmid DNA in a number of organ systems but has not bee
152 study demonstrates for the first time use of plasmid DNA in absolute quantification of malaria parasi
153 ors/Z-DNA binding protein 1 (DAI/ZBP1) bound plasmid DNA in the cytosol within 15 minutes of transfec
154 Polyphenols extracted from honeys degraded plasmid DNA in the presence of H2O2 and Cu(II) in the Fe
155 on of HPV-11, -16, and -18 origin-containing plasmid DNA in transfected cells at concentrations well
156 n this study we compared minicircle DNA with plasmid DNA in transfections of airway epithelial cells.
157 transfection of luciferase and GFP reporter plasmid DNA in vitro and in vivo under various condition
159 reconstituted pre-RCs support replication of plasmid DNA in yeast cell extracts in a reaction that ex
162 mbinase (ZFR) fusion proteins that integrate plasmid DNA into a synthetic target site in the human ge
163 ng protein that targets chromosomes, carries plasmid DNA into cells, and shows specificity for active
166 used to directly catalyze the integration of plasmid DNA into mammalian genomes, there is still an un
167 The cationic Man-CS-Phe micelles condense plasmid DNA into nanoscale polyplexes and provide effici
169 ped two polymeric gene carriers that compact plasmid DNA into small and highly stable nanoparticles w
170 IFI16 also restricted the expression of plasmid DNAs introduced by transfection but did not rest
171 n-induced single-strand-break (SSB) yield in plasmid DNA involves measurement of the fraction of rela
175 g Trojan horse liposomes (THLs), wherein the plasmid DNA is encapsulated in 100 nm pegylated liposome
178 orbent surface leads to full recovery of all plasmid DNA isoforms, which is a major issue when using
179 d, with the best-performing model applied to plasmid DNA isolated from Escherichia coli and mitochond
180 is co-transfected with transposon-containing plasmid DNA, it penetrates prokaryotic or eukaryotic cel
181 improves microparticle-based transfection of plasmid DNA lipoplexes in several primary human cell typ
182 antly enhanced survival compared to the same plasmid DNA loaded in DNA-CN in two aggressive orthotopi
186 e prophylactic and therapeutic efficacy of a plasmid DNA-mediated vaccination using the Bla g 1 gene
187 g multiple DNA nanostructures as well as two plasmid DNAs, microwaves were shown to promote the repai
188 ptide with a nucleic acid binding domain and plasmid-DNA, minicircle-DNA or small interfering RNA (si
191 ata demonstrated that the mean SSB yield per plasmid DNA molecule of [21.2+/-0.59]x10(-2)Gy(-1) as me
192 oscopy confirmed that mouse PPy/u containing-plasmid DNA molecules under the different structural str
194 -DNA nanoparticles are capable of delivering plasmid DNA of different size into cells in culture, yie
195 imultaneous delivery of siRNA and linearized plasmid DNA on the surface of a single nanocrystal provi
196 s were significantly enhanced by coating the plasmid DNA on the surface of cationic nanoparticles.
197 d by transcutaneous immunization by applying plasmid DNA onto a skin area pretreated with solid micro
198 that transcutaneous immunization by applying plasmid DNA onto a skin area wherein the hair follicles
201 ys, the transfected cell microarray, wherein plasmid DNA or siRNA, spotted on the surface of a substr
202 o this include making use of vectors such as plasmid DNA or viruses, live attenuated pathogens or sub
203 ved ORF analyses showed that the majority of plasmid DNAs originated within red algae, whereas others
207 dermal administration of nucleic acids, both plasmid DNA (pDNA) and siRNA, to treat localised disease
208 ic polyamine disulfides to condense and cage plasmid DNA (pDNA) by a process of thermodynamically con
209 on of macrophages transfected ex vivo with a plasmid DNA (pDNA) encoding a potent antioxidant enzyme,
213 roxyethyl acrylate that effectively compacts plasmid DNA (pDNA) through electrostatic binding and int
215 HIV-derived conserved element (CE) p24(gag) plasmid DNA (pDNA) vaccine is able to redirect immunodom
216 ase or angiotensin II type 2 receptor (AT2R) plasmid DNA (pDNA) was evaluated in Lewis lung carcinoma
218 dle delivery of nucleic acids, in particular plasmid DNA (pDNA), to the skin represents a potential n
220 in which the liposomes are designed to carry plasmid DNA (pDNA, containing luciferase reporter gene)
221 subcutaneous injection of cationized gelatin/plasmid DNA polyplex into the rat hindpaw and its subseq
224 e the transfection efficiency of GFP-encoded plasmid DNA (pRmHa3-GFP) into cells through efficient DN
225 Finally, DNA-BPN loaded with anti-cancer plasmid DNA provided significantly enhanced survival com
229 s more potent than deglycoBLM in supercoiled plasmid DNA relaxation, while the analogue having the di
231 imilar are not equivalent and that repair of plasmid DNA requires additional factor(s) that are not r
233 ry system can be extended to the delivery of plasmid DNA, siRNA, or aptamers for preclinical and clin
234 used different formulations of a linearized plasmid DNA solution considered for characterization as
235 1 muL droplet of whole blood, and we amplify plasmid DNA spiked into whole blood droplets to represen
236 Furthermore, the efficient amplification of plasmid DNA spiked into whole blood proves that the larg
238 A recent publication showed that circular plasmid DNA standards grossly overestimated numbers of a
239 ed LDL-cholesterol oxidation and supercoiled plasmid DNA strand breakage inhibition induced by both p
241 as both MeC and C nucleobases in transfected plasmid DNA substrates are highly susceptible to editing
244 n the successful construction of a series of plasmid DNA templates that contain many tandem copies of
245 a procedure for the isolation of total rumen plasmid DNA, termed rumen plasmidome, and subjected it t
246 Here we describe an iDNA vaccine composed of plasmid DNA that encode the full-length infectious genom
247 demonstrated superior condensing ability for plasmid DNA through the formation of compact nanosized p
248 al and liposomal vectors for the delivery of plasmid DNA to cells for gene therapy applications.
249 -walled carbon nanotubes selectively deliver plasmid DNA to chloroplasts of different plant species w
251 nanocarrier is found to effectively condense plasmid DNA to form a highly stable GNR-DSPEI-PEG-RGD/DN
252 expression, via liposomal delivery of Foxm1 plasmid DNA to Hif1a(f/f)/Tie2Cre(+) mice, resulted in r
253 ionalized fluorescent probes, antibodies, or plasmid DNA to living cells requires overcoming the plas
254 (DAB) dendrimer would allow the transport of plasmid DNA to the brain after intravenous administratio
255 imine dendrimer would allow the transport of plasmid DNA to the brain after intravenous administratio
258 f PEI can be effective delivery vehicles for plasmid DNAs to elicit cellular and humoral protective r
259 ut, contributing to a 2.5~3 fold increase on plasmid DNA transfection and an additional 10-55% transg
261 s for gene therapy of airway disorders where plasmid DNA transfections have so far proven inefficient
264 ase of the bacterium (OD 6.0) using 25 ng of plasmid DNA under 100 Ohms resistance and 1.7 kV/cm volt
266 ounds exhibited significant photocleavage of plasmid DNA upon visible light irradiation and are rapid
267 ) and microRNA-125b-2 (miR-125b2) expressing plasmid DNA using hyaluronic acid-poly(ethylene imine)/h
270 an influenza challenge model we show that a plasmid DNA vaccine complexed to a less toxic form of PE
271 vatives of PEI could be utilised for topical plasmid DNA vaccine delivery to human mucosal tissue sur
273 IV-1 CN54gp140 antigen when cation-complexed plasmid DNA vaccines are applied topically to the murine
276 te nanotopography of silica nanoparticles as plasmid DNA vectors has significant impact on the transf
277 Efficient in vitro electrotransfection of plasmid DNA was demonstrated in several hard-to-transfec
283 led to a luciferase bioluminescence reporter plasmid DNA were shown to transfect tumors implanted in
284 and the mobilities of circular versus linear plasmid DNAs were also affected by the chemical form and
285 are incorporated into cells or organisms as plasmid DNA, which leads the transcriptional and transla
286 sition of horizontally transferred phage and plasmid DNA, which provides virulence and resistance fun
287 an be derived from biologically synthesized (plasmid) DNA, which reduces errors associated with chemi
288 rR links the filaments with centromeric parC plasmid DNA, while facilitating the addition of subunits
289 anostructures and single-stranded regions of plasmid DNA, while intense THz pulses had the opposite e
290 (TopA67) but not full-TopA in the absence of plasmid DNA, while PrS-YjhX binds to full-TopA in the pr
291 orphologies are prepared via condensation of plasmid DNA with a block copolymer of polyethylene glyco
294 ting stem-loop was confirmed by digestion of plasmid DNA with apurinic endonuclease IV, followed by p
298 nd simple system for delivering oncolytic Ad plasmid DNA with the bioreducible polymers, skipping tim
299 nthetic nanonucleases, able to cleave pBR322 plasmid DNA with the highest efficiency reported so far
300 developed a novel system using oncolytic Ad plasmid DNA with two bioreducible polymers: arginine-gra