ライフサイエンスコーパス: pollen tube

1 on at the interface of the synergid cell and pollen tube.

2 ll and, thus, to the polarized growth of the pollen tube.

3 sperm cells and their transport vehicle, the pollen tube.

4 mediated endocytosis and polar tip growth in pollen tubes.

5 complemented the aberrant growth of exo70C2 pollen tubes.

6 (4,5)P2] is critical for polar tip growth of pollen tubes.

7 et foci at the shank membrane of Arabidopsis pollen tubes.

8 ulation of optimal tip growth of Arabidopsis pollen tubes.

9 lementation at the apical plasma membrane of pollen tubes.

10 ls could not sustain the growth of wild-type pollen tubes.

11 ylinositol 4,5-bisphosphate (PIP2) marker in pollen tubes.

12 tubes, whereas NIP4;2pro:GUS flowers only in pollen tubes.

13 required to sustain the high growth rates of pollen tubes.

14 ments were less organized in myo11c1 myo11c2 pollen tubes.

15 y, actin organization, and optimal growth of pollen tubes.

16 specifically enriched in in vivo-elongating pollen tubes.

17 f Ca(2+) oscillations in the polar growth of pollen tubes.

18 tein kinase, CPK32, controls polar growth of pollen tubes.

19 e extracellular matrix inhibit interspecific pollen tubes.

20 LRX8 in vitro and RALF4 function in growing pollen tubes.

21 al membrane traffic at the tip of elongating pollen tubes.

22 d by straight-billed territorial birds (mean pollen tubes = 0.20 +/- 0.074 SE) or insects.

23 ingbird species with specialized bills (mean pollen tubes = 1.21 +/- 0.12 SE) but was reduced 5.7 tim

24 ess this hypothesis, we employed the growing pollen tube, a well-established cell polarity model syst

25 llination of Heliconia tortuosa (measured as pollen tube abundance) occurred frequently when plants w

26 Different combinations of the timing of pollen-tube access to limiting stylar resources (simulta

27 myb triple-mutant pollen tubes also fail to release their sperm cargo.

28 by FERONIA in which the arrival of the first pollen tube alters ovular conditions to disengage pollen

29 plants via ALMT, including the regulation of pollen tube and root growth, and that GABA can finally b

30 atus, the first point of contact between the pollen tube and the female gametophyte.

31 ell-to-cell communication events between the pollen tube and the female reproductive tissues, which a

32 ensive communication takes place between the pollen tube and the female tissues until the sperm cell

33 zed cells flanking the egg cell that attract pollen tubes and degenerate upon pollen tube arrival.

34 at embryo sacs sense the imminent arrival of pollen tubes and respond by generating an oxidative envi

35 Pollen tubes and root hairs are model cell systems for s

36 Tip growth of pollen tubes and root hairs occurs via rapid polar growt

37 ules that multiplied to confer tip growth in pollen tubes and root hairs, respectively.

38 amics of HG during elongation of Arabidopsis pollen tubes and root hairs.

39 P. patens tip-growing cells and A. thaliana pollen tubes and root hairs.

40 ing question for various cell types, such as pollen tubes and root hairs.

41 rol of cell size, polarity and the growth of pollen tubes and root hairs.

42 enzyme was present in the growing region of pollen tubes and that the enzyme colocalizes with cell w

43 communication between the male gametophyte (pollen tube) and the maternal tissues of the flower, cul

44 cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the veg

45 localized in the cytoplasm of pollen grains, pollen tubes, and also root trichoblast cells.

46 growing cells, such as animal neurons, plant pollen tubes, and root hairs.

47 ntrolled by the cortical actin fringe at the pollen tube apex.

48 nergid cells after physical contact with the pollen tube apex.

49 ar below the maximum penetration force these pollen tubes are able to generate.

50 In Arabidopsis, pollen tubes are guided by cysteine-rich chemoattractant

51 Pollen tubes are highly polarized tip-growing cells that

52 Furthermore, HT-B levels within the pollen tubes are reduced when NaStEP-suppressed pistils

53 Pollen tube arrival at the ovule triggers the accumulati

54 hat attract pollen tubes and degenerate upon pollen tube arrival.

55 Using pollen tubes as a developmental model, we showed that th

56 A inhibited clathrin-mediated endocytosis in pollen tubes associated with an increased accumulation o

57 NIA (FER) and MYB98, which are necessary for pollen tube attraction and perception, remain unaffected

58 n tube alters ovular conditions to disengage pollen tube attraction and prevent the approach and pene

59 t have greatly advanced our understanding of pollen tube attraction strategies and the mechanisms tha

60 in ovular sporophytic tissue is involved in pollen tube attraction, and promotes secretion of the po

61 d interaction with its receptor, to suppress pollen tube attraction.

62 develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae.

63 identification of genes important in in vivo pollen tube biology.

64 including micropylar guidance (IV6 and IV4), pollen tube burst (IV2), and repulsion of multiple polle

65 hort [Ca2+]cyto transient is associated with pollen tube burst and sperm cell arrival.

66 female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations.

67 fertility caused by the rescue of anx1 anx2 pollen tube bursting.

68 taken up by both compatible and incompatible pollen tubes, but its suppression in Nicotiana spp. tran

69 ells had lower arabinose levels in roots and pollen tubes, but no differences were observed in GalA o

70 rs after intensive communication between the pollen tube cell and the receptive synergid, culminating

71 tility and are transported by the vegetative pollen tube cell for fertilization, but the extent to wh

72 ire transportation as a passive cargo by the pollen tube cell to the egg apparatus (egg cell and acce

73 The modification of the pollen tube cell wall by the pistil, then, is likely a k

74 Involvements from pollen tube cell wall components 690 VI.

75 be attraction, and promotes secretion of the pollen tube chemoattractant LURE1.2.

76 te at the filiform apparatus, where arriving pollen tubes communicate with the embryo sac.

77 The walls of Nicotiana alata pollen tubes contain a linear arabinan composed of (1,5)

78 However, exo70C2 pollen tubes could frequently recover and restart their

79 ically expressed and delivered LRE-cYFP from pollen tubes could non-cell-autonomously complement the

80 In the pollen tube, cytoplasmic TGases are likely to be involve

81 ted exocytosis-related functional effects in pollen tubes despite the absence of apparent plasma memb

82 ve cell-specific functions, in root hair and pollen tube development, for example.

83 the mutant allele resulting from defects in pollen tube development, indicating that TAF1 is importa

84 that sperm cells are dispensable for normal pollen tube development.

85 known of the transcriptome of in vivo-grown pollen tubes due to the difficulty of collecting pollen

86 is required for the stability of HT-B inside pollen tubes during the rejection response, but the unde

87 has been used to identify genes expressed in pollen tubes elongating in vitro; however, little is kno

88 n development, pollen grain germination, and pollen tube elongation are crucial biological processes

89 errelated, and all of them are essential for pollen tube elongation in pistil, especially, free Ca(2+

90 stream pH-dependent mechanisms essential for pollen tube elongation, and thus plant fertility.

91 cific PMEs are traditionally associated with pollen tube elongation, this study provides strong evide

92 nation of metabolites during germination and pollen tube elongation.

93 EI4 and AtPMEI9 had distinct consequences on pollen tube elongation.

94 AtLURE1s instead function as pollen tube emergence accelerators that favor conspecifi

95 may modify the pollen cell wall and regulate pollen tube emergence and growth.

96 en attachment site, for pollen hydration and pollen tube entry.

97 their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain polle

98 In this study, we identified two Arabidopsis pollen-tube-expressed glycosylphosphatidylinositol-ancho

99 Additionally, pollen tube-expressing LRE variants lacking domains crit

100 horylation of purified recombinant PIP5K6 by pollen tube extracts could be attributed to MPK6.

101 Wild-type pollen tubes failed to arrest growth and to rupture afte

102 eplaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregula

103 imely tube rupture at the spatially confined pollen tube-female gametophyte interface.

104 Pollen tubes first grow within the cell wall of the papi

105 relies on the highly regulated growth of the pollen tube for sperm delivery.

106 smaller than the maximum force generated by pollen tubes from L. longiflorum (36 uN).

107 enetrate into a stiffer matrix compared with pollen tubes from L. longiflorum, even though the maximu

108 By contrast, pollen tubes from Lilium longiflorum and other plant spe

109 , even though the maximum force generated by pollen tubes from N. tabacum (11 uN) is smaller than the

110 ransition from a softer to a stiffer matrix, pollen tubes from N. tabacum display a greater ability t

111 We found that pollen tubes from Nicotiana tabacum and other plant spec

112 durotropic growth, that is only expressed in pollen tubes from plants with a solid or semisolid trans

113 Here we show that pollen tubes from plants with solid transmitting tracts

114 anslation (the translatome) of in vivo-grown pollen tubes from self-pollinated gynoecia of Arabidopsi

115 Our work defines the pollen tube gene products that respond to the pistil and

116 In particular, myo11c1 myo11c2 pollen tubes grew more slowly than wild-type pollen tube

117 (TM) proteins in tobacco (Nicotiana tabacum) pollen tubes growing normally or treated with Brefeldin

118 Mutant pollen tubes grown in vitro exhibited an enhanced growth

119 Cooperation between ovule attraction and pollen tube growth acceleration favors conspecific ferti

120 Effects of PIP5K6 expression on pollen tube growth and cell morphology were attenuated b

121 Calcium oscillations control pollen tube growth and fertilization in flowering plants

122 utocrine and paracrine signaling to maintain pollen tube growth and induce timely tube rupture at the

123 roduction, which fuels single nucleus-driven pollen tube growth and is essential for plant reproducti

124 ere, we show that mat3 mutants have impaired pollen tube growth and reduced seed set.

125 gk4-1/+ double heterozygote showed defective pollen tube growth and seed development because of nonvi

126 reeding system was determined by analysis of pollen tube growth and seed production from controlled p

127 TPases (AHAs) have been proposed to energize pollen tube growth and underlie cell polarity, however,

128 sion and guidance for pollen germination and pollen tube growth are abundantly present in the extrace

129 Pollen tube growth assays in vitro and in the pistil dem

130 haliana delays pollen germination and causes pollen tube growth defects, leading to drastically reduc

131 Ca(2+) availability partially suppresses the pollen tube growth defects, suggesting that LRX proteins

132 e by examining seed set, pollen fitness, and pollen tube growth for knockout mutants of five of the s

133 ulted in reduced pollen adhesion and delayed pollen tube growth in all mutants studied.

134 abacum resulted in the loss of inhibition of pollen tube growth in Nicotiana obtusifolia (synonym Nic

135 pollen development, pollen germination, and pollen tube growth in other species.

136 nscript levels during stamen development and pollen tube growth in the transgenic trees of a stamen-s

137 nteraction of cognate PrsS and PrpS triggers pollen tube growth inhibition and programmed cell death

138 s vital and in vitro germination normal, but pollen tube growth inside stylar tissues appeared less d

139 Pollen tube growth is an essential aspect of plant repro

140 w both in vitro and in vivo that asf1 mutant pollen tube growth is stunted, limiting fertilisation to

141 ion of the exocytotic machinery depending on pollen tube growth modes.

142 , a sugar alcohol, in flower development and pollen tube growth of apple (Malus domestica).

143 tigmas, pollen development, pollination, and pollen tube growth require spatial and temporal regulati

144 ubstantiating a mechanistic role for AHAs in pollen tube growth through plasma membrane hyperpolariza

145 Pollen Receptor-Like Kinases (PRKs) control pollen tube growth through the pistil in response to ext

146 an essential role in stamen development and pollen tube growth via MdMYB39L in apple.

147 Polarized exocytosis is critical for pollen tube growth, but its localization and function ar

148 use severe defects in pollen germination and pollen tube growth, resulting in a reduced seed set.

149 its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes

150 ecreased pollen germination rate and reduced pollen tube growth, which were all closely related to lo

151 ss effects on pollen hydration, adhesion and pollen tube growth.

152 expression of its putative target genes and pollen tube growth.

153 ze the mitochondria and contribute to arrest pollen tube growth.

154 ect (segregation 40%:51%:9%) due to aberrant pollen tube growth.

155 ough the male gametophyte due to a defect in pollen tube growth.

156 pollen, while NIP4;2 expression peaks during pollen tube growth.

157 he ER and were involved in PA production for pollen tube growth.

158 luble inorganic pyrophosphatase required for pollen tube growth.

159 imilar pollen myosins had greater defects in pollen tube growth.

160 ect leading to more severe depolarization of pollen tube growth.

161 e cell walls that provide less resistance to pollen tube growth.

162 l-associated TGases are believed to regulate pollen tube growth.

163 en maturation, pollen grain germination, and pollen tube growth.

164 ck mechanism for calcium oscillations during pollen tube growth.

165 ng wound signaling, stomatal regulation, and pollen tube growth.

166 processes and sexual reproduction, namely in pollen tube guidance and embryo sac fertilization, patho

167 es the high robustness and responsiveness of pollen tube guidance and explains the connection between

168 a(2+) providing a concentration gradient for pollen tube guidance and involved in signal transduction

169 by the endogenous promoter caused disturbed pollen tube guidance and reduced fertility.

170 esults reveal an unexpected role for KTN1 in pollen tube guidance on the stigma by ensuring mechanica

171 issues of the flower, culminating in precise pollen tube guidance to the female gametophyte (embryo s

172 ract and thus may contribute to an effective pollen tube guidance within the pistil.

173 t1 mutants show severe dwarfism, compromised pollen tube guidance, and constitutive activation of sal

174 ion, physical mechanisms also play a role in pollen tube guidance; however, these processes remain po

175 lassical AGP function as a Ca(2+) capacitor, pollen tube guide and wall plasticizer into a simple but

176 Furthermore, mutant pollen tubes had less negative membrane potentials, subs

177 en female tissues of the pistil and paternal pollen tubes imposes hybridization barriers in flowering

178 The pollen tube in a flowering plant grows in a direction th

179 netration of female gametophytes by multiple pollen tubes in Arabidopsis.

180 tube burst (IV2), and repulsion of multiple pollen tubes in embryo sac (IV2).

181 By manipulating the [pH]cyt of the pollen tubes in vivo, we show that [pH]cyt acidification

182 OP1 GTPase, a key regulator of tip growth in pollen tubes, in an opposite manner.

183 MeSA, are localized at the apical region of pollen tubes, indicating of the tip-localized production

184 pollen grains disrupts the SI by preventing pollen tube inhibition.

185 rgid cell and the coincident bursting of the pollen tube inside the ovule to release the sperm.

186 ) was reported to control the maintenance of pollen tube integrity by perceiving the autocrine peptid

187 Here, we review recent progress related to pollen tube integrity control, mainly focusing on the mo

188 tube-expressed and are required to maintain pollen tube integrity.

189 In pollen tubes, interaction between RAC5 and RISAP is dete

190 A pollen tube is a single cell that only grows at the tip,

191 amnogalacturonan I, the arabinan in N. alata pollen tubes is considered free, as there is no detectab

192 rate that the growth guidance of Arabidopsis pollen tubes is regulated by the signaling network that

193 brane trafficking and inhibits exocytosis in pollen tubes; it also leads to the degradation of the ac

194 Rooting cells and pollen tubes-key adaptative innovations that evolved dur

195 Pollen tubes lacking these three transcriptional regulat

196 r silique and reduced pollen germination and pollen tube length.

197 Root hair cells and pollen tubes, like fungal hyphae, possess a typical tip

198 Pollen tube membrane-localized receptors coordinate cell

199 StEP and NaSIPP interaction, in incompatible pollen tubes, might destabilize the mitochondria and con

200 To reach the female gametophyte, growing pollen tubes must penetrate different tissues within the

201 593 to the globular tail domain of a tobacco pollen tube myosin XI.

202 highlight the signaling systems required for pollen tube navigation and the potential roles of Ca(2+)

203 Sperm cell release from the pollen tube occurs after intensive communication between

204 ell wall of the pollen grain and the growing pollen tube of plasmolyzed pollen.

205 Pollen tubes of aha mutants had reduced extracellular pr

206 wth by oversecretion of CW material, whereas pollen tubes of anx1 anx2 double mutants burst spontaneo

207 novel, as LRE was not expressed in pollen or pollen tubes of mutants defective for MET1, DDM1, RNA-de

208 r to pollination, the growth of incompatible pollen tubes of N. obtusifolia and N. repanda was inhibi

209 In root hairs and pollen tubes of the seed plant Arabidopsis thaliana, cel

210 ccurs during the formation of root hairs and pollen tubes or de novo formation of cell plates during

211 mics analyses confirmed that mat3 pollen and pollen tubes overaccumulate Met and that mat3 pollen has

212 r guided growth of the male gametophytes and pollen tube-ovule interaction.

213 In lily (Lilium formosanum) pollen tubes, pectin, a major component of the cell wall

214 expression, from first pollen mitosis up to pollen tube penetration in the ovule, thereby revealing

215 of pollen grain adhesion, pollen hydration, pollen tube penetration, seed set, and overall fertility

216 oforms localized to different regions of the pollen tube plasma membrane, apical vesicle-rich inverte

217 rowth associate with distinct regions of the pollen tube PM, and understanding the mechanisms respons

218 ,5)P2 production and membrane trafficking in pollen tubes, possibly contributing to directional growt

219 len tubes, where potential interactions with pollen tube proteins might underlie its function.

220 between synergid cells and a tip-elongating pollen tube (PT) for the successful delivery of sperm ce

221 One of the two sperm delivered by the pollen tube (PT) fuses with the egg cell to form the zyg

222 gical implications of anion transport during pollen tube (PT) growth in Arabidopsis thaliana (Col-0).

223 ubfamily, are required for tip growth of the pollen tube (PT), and their closest homolog, FERONIA, co

224 In plants, NO is generated in pollen tubes (PTs) and affects intracellular responses t

225 n addition to the lack of Hyp-Ara in hpat1/3 pollen tubes (PTs), we also found broadly disrupted cell

226 ng plants (angiosperms) are characterized by pollen tubes (PTs; male gametophytes) carrying two immob

227 are self-sterile despite the fact that self-pollen tubes reach the ovary and in most cases penetrate

228 cell surface and known to critically control pollen tube reception [6].

229 erefore, LRE and FERONIA jointly function in pollen tube reception at the interface of the synergid c

230 l-anchored membrane protein, is critical for pollen tube reception by the female gametophyte before f

231 In angiosperms, pollen tube reception by the female gametophyte is requi

232 s could non-cell-autonomously complement the pollen tube reception defect in lre female gametophytes,

233 transmembrane domain, fully complemented the pollen tube reception defect in lre-7 female gametophyte

234 Arabidopsis thaliana lorelei (lre) mutants, pollen tube reception fails in most female gametophytes,

235 Several important factors involved in the pollen tube reception have been identified recently [2-6

236 ring plants, the female gametophyte controls pollen tube reception immediately before fertilization a

237 sing LRE-cYFP showed that the role of LRE in pollen tube reception requires the M8CM, but not the dom

238 proteins (ENODLs, or ENs), are required for pollen tube reception.

239 role of ENs in male-female communication and pollen tube reception.

240 ent, inefficient SC delivery, and defects in pollen tube reception.

241 ternal peptides XIUQIU1 to -4, which attract pollen tubes regardless of species.

242 Rhizoids, root hairs, and pollen tubes respond similarly to disruption of MLR and

243 metophyte induces rupture of the penetrating pollen tube, resulting in sperm release and rendering th

244 Tip growth has been studied in pollen tubes, root hairs, and fungal and oomycete hyphae

245 cals in the female gametophyte, which causes pollen tube rupture and sperm cell release during fertil

246 BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release.

247 entrance to the female gametophyte to induce pollen tube rupture and sperm release.

248 t reactive oxygen molecules, and they induce pollen tube rupture in a Ca(2+)-dependent process involv

249 ts, whose female gametophytes fail to induce pollen tube rupture, to decipher the molecular mechanism

250 Mutant pollen tubes rupture early during the pollination proces

251 It was further hypothesized that pollen-tube rupture to release sperm is caused by the pa

252 dy-state growing tobacco (Nicotiana tabacum) pollen tubes, SEC3a displayed amino-terminal Pleckstrin

253 Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of M

254 n the model tip growth system of Arabidopsis pollen tubes, several small-molecule hormones regulate t

255 zes to punctae at the plasma membrane of the pollen tube shank, which are stably associated with cort

256 Coexpression of NaStEP and NaSIPP in pollen tubes showed interaction in the mitochondria, alt

257 We report that pollen-tube-specific cyclic nucleotide-gated channels (C

258 Upon arrival at the female gametophyte, the pollen tube stops growing and releases sperm cells for s

259 or after) create signature relations of mean pollen-tube success and its variation among pistils to p

260 During this process, a pollen tube surface-located receptor complex composed of

261 molecules interacting with receptors at the pollen tube surface.

262 How the pollen tube switches from a rapid polarized growth towar

263 germination, the vegetative cell extrudes a pollen tube that carries the sperm to an ovule for ferti

264 s at the tip and along the shank of N. alata pollen tubes that are predominantly part of the primary

265 ng male sterility defect as a consequence of pollen tubes that fail to fully elongate following polli

266 self S-RNases, but not self S-RNases, in the pollen tube, thereby resulting in cross-compatible polli

267 creasing mechanical impedance was met by the pollen tubes through modulation of the cell wall complia

268 iosperms depends on the proper trajectory of pollen tubes through the pistil tissues to reach the ovu

269 entified various chemotropic cues that guide pollen tubes through the transmitting tract of the pisti

270 n of the female gametophyte by late-arriving pollen tubes, thus averting polyspermy.

271 Occam's Razor suggests a new model of pollen tube tip growth based on a novel Hechtian oscilla

272 Pollen tube tip growth depends on balancing secretion of

273 In tobacco (Nicotiana tabacum), pollen tube tip growth is controlled by the RAC/ROP GTPa

274 was during germination and the initiation of pollen tube tip growth.

275 mpartment, whose cytoplasmic position at the pollen tube tip is maintained by the actin cytoskeleton.

276 tional and accumulates at or proximal to the pollen tube tip plasma membrane.

277 Highly polarized secretion at a growing pollen tube tip requires the exocyst tethering complex r

278 ead being delivered in a wide arc across the pollen tube tip.

279 ns and later to the apical domain in growing pollen tube tips characterized by intensive exocytosis.

280 d-labeled vesicles and their accumulation at pollen tube tips were not affected in the myo11c1 myo11c

281 that are required for sperm release from the pollen tube to the female gametes, a critical barrier to

282 m cells are transported and delivered by the pollen tube to the ovule to achieve double fertilization

283 receptor PRK6 aid attraction of the growing pollen tube to the ovule.

284 apilla cells is accompanied by a tendency of pollen tubes to coil around the papillae.

285 self-propelled sperm cells (SCs), plants use pollen tubes to deliver SCs, in which the pollen vegetat

286 hat, coordinately, allow root hair cells and pollen tubes to expand in a controlled manner to several

287 C S. pennellii LA0716 allows S. lycopersicum pollen tubes to penetrate farther into the pistil in HT

288 SC S. arcanum LA2157 allows S. lycopersicum pollen tubes to penetrate to the ovary and produce hybri

289 These results indicate a strategy of pollen tubes to protect microtubules and avoid growth ar

290 owering plants, sperm are transported inside pollen tubes to the female gametophyte for fertilization

291 Flowering plants rely on pollen tubes to transport their immotile sperm to fertil

292 In flowering plants, pollen tubes undergo tip growth to deliver two nonmotile

293 ns as well as in vivo- and in vitro-cultured pollen tubes were assayed by microarray analyses, reveal

294 ed in the cell wall of pear (Pyrus communis) pollen tubes, where it might play a role in the regulati

295 During pollination, NaStEP is taken up by pollen tubes, where potential interactions with pollen t

296 ers showed GUS activity in mature pollen and pollen tubes, whereas NIP4;2pro:GUS flowers only in poll

297 nd they are embraced as passive cargoes by a pollen tube which embarks on a long journey in the pisti

298 pollen tubes grew more slowly than wild-type pollen tubes, which resulted in reduced fitness compared

299 asma membrane in the shank region of growing pollen tubes, which we have termed Actin-Membrane Contac

300 zed as the site of exocytosis in the tobacco pollen tube, while NtEXO70B1 surprisingly colocalized wi

301 is more complex, involving interaction of a pollen tube with egg adjoining synergid cells, culminati

302 ion of transcripts engaged with polysomes in pollen tubes within self-fertilized florets has identifi