ライフサイエンスコーパス: polybasic

1 by an electrostatic compensation between the polybasic 23-30 region and the alpha3 electronegative su

2 This depends on the polybasic activation loop as well as a conserved hydroph

3 We identified a role for a conserved polybasic amino acid motif in an N-terminal domain previ

4 Peptide mapping identified polybasic amino acid motifs in the proenzyme responsible

5 ma membrane and depends on the presence of a polybasic amino acid segment at the N terminus of PrP.

6 he intracerebral pathogenicity index and the polybasic amino acid sequence at the fusion protein clea

7 en characterized, but, because it contains a polybasic amino acid sequence, it potentially interacts

8 A sequence analysis revealed polybasic amino acids in the hemagglutinin connecting pe

9 Its effects involve both a polybasic amino-terminal segment and a proline-rich core

10 We removed the polybasic aminoacids that are associated with high virul

11 CaM specifically targeted the highly polybasic anchor region of the K-Ras4B HVR that stably w

12 The C-terminal polybasic anchors of RAP1A and RAP1B differ in their ami

13 ply that relevant FTI targets will lack both polybasic and potentially geranylgeranylated methionine-

14 min-1 binds to the SNARE complex through the polybasic and primary interfaces in solution.

15 tes Orai1 by a different mechanism since the polybasic and S/P domains of STIM1 are not required for

16 Cleavage of S generates a polybasic Arg-Arg-Ala-Arg carboxyl-terminal sequence on

17 ration of hitherto unknown highly acidic and polybasic bis(difluoromethylene)triphosphoric acid 1 usi

18 was present, supporting a role for the Rac1 polybasic C terminus in binding to the membrane.

19 both the Ras homology (core) domain and the polybasic C terminus.

20 proteins from human H1N1 strains that lack a polybasic cleavage motif.

21 proteins of the H5 and H7 subtypes that have polybasic cleavage motifs, this study demonstrates that

22 matory pathways more than the removal of the polybasic cleavage site alone.

23 n influenza virus HA proteins that contain a polybasic cleavage site from pH-induced conformational c

24 sion of a hemagglutinin protein in which the polybasic cleavage site had been removed.

25 The absence of the polybasic cleavage site in SARS-CoV-2 did not affect vir

26 l potentially recombinant coronavirus with a polybasic cleavage site in the S protein, dominant in RC

27 l potentially recombinant coronavirus with a polybasic cleavage site in the spike (S) protein.

28 In contrast, introduction of a polybasic cleavage site into Israel810 HA leads to pseud

29 To this end, we introduced a polybasic cleavage site into the HA of several low-patho

30 f SARS-CoV-2 that either directly affect the polybasic cleavage site itself (NSPRRAR) or a flanking s

31 rin cleavage of the spike protein at R682, a polybasic cleavage site that differs from the SARS-CoV s

32 ines expressing either HPAIV HA in which the polybasic cleavage site was replaced with that from a lo

33 ed to distinguish between compatibility of a polybasic cleavage site with H5/H7 HA only and unique pr

34 Furthermore, we uncovered a polybasic cleavage site, RARR, in the spike (S) protein

35 cytin-2 to a leucine residue upstream of the polybasic cleavage site.

36 human influenza virus HA proteins that lack polybasic cleavage sites.

37 low-pathogenic ancestors via acquisition of polybasic cleavage sites.

38 termini, containing N-myristate and either a polybasic cluster (in Src) or palmitoylation sites (e.g.

39 uently, C2B readily associates with PM via a polybasic cluster and a Ca(2+)-binding loop.

40 and 1.9 A, respectively, unveiling that the polybasic cluster formed by strands beta3-beta4 is invol

41 Similarly, mutations in the polybasic cluster of MA that disrupt Gag polarization ca

42 he presence of a key glutamic residue in the polybasic cluster of synaptotagmin 1 that abolishes the

43 The C-terminal polybasic cluster of the Rab35 HVD is essential for plas

44 requires a pleckstrin homology domain and a polybasic cluster that bind to phosphoinositide lipids.

45 ion is mediated by an HE motif followed by a polybasic cluster that is conserved in transcriptional C

46 To test whether these polybasic clusters bind negatively charged phosphatidyli

47 Unexpectedly, proteins with polybasic clusters dissociated from the PM only when bot

48 rimary structure of this motif is similar to polybasic clusters known to interact with polyphosphoino

49 Polybasic cofactors lowered the K(m) for diacylglycerol

50 ies showed that acidic phospholipids recruit polybasic cofactors to the vesicle surface but have litt

51 afts through electrostatic interactions with polybasic cytoplasmic proteins, such as GAP-43, which bi

52 a hybrid anchor that comprises a lysine-rich polybasic domain (PBD) and a C-terminal farnesyl chain.

53 The lipin proteins each contain a conserved polybasic domain (PBD) composed of nine lysine and argin

54 (PH) domain and an adjacent carboxy-terminal polybasic domain [3] [9].

55 The polybasic domain alone increases the affinity of Ras for

56 targeting R7BP to the plasma membrane with a polybasic domain and an irreversibly attached lipid inst

57 es, was replaced with that of the human Rac2 polybasic domain containing only three basic residues, a

58 d PI(4,5)P(2) Electrostatic targeting by the polybasic domain contributes significantly to the PM loc

59 Thus, polybasic domain differences account for the disparate a

60 Here we demonstrate that a polybasic domain immediately upstream of palmitoylated c

61 These data demonstrate the key role of the polybasic domain in controlling stress-regulated exon pa

62 FHOS interacted with the polybasic domain in the Rac1 C terminus in a guanine nuc

63 olycysteine sequences, and that the adjacent polybasic domain is not required for Galpha palmitoylati

64 Thus, the composition of the polybasic domain is sufficient for determining Rac isofo

65 ent and prenyl chain length modifies nascent polybasic domain lipid preferences.

66 have taken a closer look at the role of the polybasic domain of Cdc42 in its ability to bind to memb

67 ltiple substitutions within the hydrophilic, polybasic domain of gp91(phox) encompassed by residues 8

68 phosphorylation regulates the ability of the polybasic domain of lipin 1 to recognize di-anionic PA a

69 Our studies suggest that the polybasic domain of Rac is a novel effector domain that

70 Moreover, replacing the highly charged polybasic domain of Rac1 with the less charged domain of

71 -Ras polybasic domain, we show that either a polybasic domain or an alternatively prenylated CAAX ren

72 A polybasic domain proximal to the CaaL box motif induced

73 n of a negatively charged phosphate into the polybasic domain reduced interaction of ARNO with membra

74 se C; (d) reveal that phosphorylation of the polybasic domain regulates affinity for F-actin and Ca(2

75 ic amino acids to acidic residues within the polybasic domain results in inhibition of channel palmit

76 t that palmitoylation of the AKAP N-terminal polybasic domain targets it to postsynaptic lipid rafts

77 itic spine plasma membranes by an N-terminal polybasic domain that binds phosphoinositide lipids, F-a

78 aPKC, Dlg also contains a positively charged polybasic domain that electrostatically binds the PM pho

79 KChIP2c splice isoforms that lack a putative polybasic domain that is present in longer KChIP2a1 and

80 of the STIM1 C terminus, thereby releasing a polybasic domain that promotes STIM1 recruitment to ER-P

81 expression of a Rac2 derivative in which the polybasic domain was replaced with that of Rac1 did not

82 s electrostatic interaction of the lipidated polybasic domain with anionic phospholipids in the plasm

83 ster of positively charged residues (i.e. a "polybasic domain"), directly preceding their geranylgera

84 ared to be mediated by the carboxyl-terminal polybasic domain, and the specific GTPase-activating eff

85 STIM1DeltaK, which lacks the PIP(2)-binding polybasic domain, was recruited to ER-PM junctions follo

86 ons of Ras background, CAAX motif, and K-Ras polybasic domain, we show that either a polybasic domain

87 ic Rac1 protein in which the Rac1 C-terminal polybasic domain, which contains six lysines or arginine

88 single serine residue within the core of the polybasic domain, which results in channel inhibition, a

89 cal interaction of PIPKI-alpha with the Rac1 polybasic domain.

90 itively charged STIM1((684)KK(685)) in STIM1 polybasic domain.

91 undergoes monoubiquitination in a conserved polybasic domain.

92 e, S392, located within the carboxy-terminal polybasic domain.

93 ly GTPases mediated by the carboxyl-terminal polybasic domain.

94 rises a farnesyl-cysteine-methyl-ester and a polybasic domain.

95 phorylates K-Ras at Ser181 in the C-terminal polybasic domain.

96 fication that operates in conjunction with a polybasic domain.

97 et of the plasma membrane via a farnesylated polybasic domain; however, the structural details of the

98 by single-point mutations in the respective polybasic domains (PBD).

99 In particular, polybasic domains represent an attractive mechanism to d

100 le lipid modification, or hydrophobic and/or polybasic domains.

101 omers is the composition of their C-terminal polybasic domains.

102 y oligocations such as polyamines, melittin, polybasic drugs, oligolysines, and oligoarginines.

103 We investigated the hypothesis that the polybasic effector domain (ED) of the abundant intracell

104 the combined action of myristoylation and a polybasic effector domain, which binds phospholipids and

105 A K720E mutation in the polybasic face and a K706E mutation in the C(2)B domain

106 For the C2B domain, contact occurs in the polybasic face and sites opposite the Ca(2+)-binding loo

107 switch to modulate the accessibility of the polybasic face of C2B and control interactions of syt1 w

108 The polybasic face of C2B does not interact with the membran

109 ith the plasma membrane: (i) one involving a polybasic face that is expected to yield a perpendicular

110 It contains a polybasic farnesylated C-terminus that is required for t

111 tering into microdomains are mediated by its polybasic farnesylated C-terminus.

112 First, the virus lacks the canonical polybasic furin cleavage signal in the fusion (F) glycop

113 in bats and pangolins, SARS-CoV-2 harbors a polybasic furin cleavage site in its spike (S) glycoprot

114 A polybasic furin-cleavage site (FCS) in spike, and evolut

115 All isolates had polybasic fusion (F)-protein cleavage sites and were sho

116 Taken together, in the presence of a polybasic HA cleavage site, non-H5/H7 HA can support a h

117 he HA serotypes H5 or H7 by acquisition of a polybasic HA cleavage site.

118 r unique predisposition for acquisition of a polybasic HA cleavage site.

119 loid deposits, relative to similarly charged polybasic heparin-reactive peptides, because it adopted

120 ity enables CaM to orient efficiently to the polybasic HVR anchor, which is partially diffused into t

121 SARS-CoV-2 has a polybasic insertion (PRRAR) at the S1/S2 cleavage site t

122 onical form (ATXbeta) in having a 52-residue polybasic insertion of unknown function in the catalytic

123 The exact function of the polybasic juxtamembrane region (5RK) of the plasma membr

124 ith phosphoinositides, the function of Syx's polybasic juxtamembrane region (5RK) remains unclear.

125 AREDelta60, but the interaction requires the polybasic juxtamembrane region of syntaxin-1 and is not

126 Our findings highlight the importance of the polybasic juxtamembrane sequence in regulating the oncog

127 harge neutralization, upon modification of a polybasic linker known to direct NLRP3 Golgi association

128 hat interacts with a separate less-conserved polybasic linker region of the protein.

129 In the absence of PIP 2, the polybasic lipid binding site on the beta3-beta4 hairpin

130 ven by three critical domains (NTD, CTD, and polybasic LMN).

131 cherichia coli) binds to PtdIns(4,5)P2 via a polybasic lysine patch in the C2B domain, which may prom

132 dSer, and an increase in the affinity of the polybasic lysine patch to phosphatidylinositol-4,5-bisph

133 trate sites within the AKAP79/150 N-terminal polybasic membrane-cytoskeletal targeting domain were ph

134 Here we show that a membrane-proximal, polybasic motif (PBM) in the cytosolic tail of p14 is es

135 We recently reported that a polybasic motif (PBM) in the cytosolic tail of reptilian

136 lserine (PS)-containing bilayers through its polybasic motif (PBM).

137 ree distinct membrane-interacting regions: a polybasic motif (R.RKTR) from the regulatory alphaC-heli

138 izes to the plasma membrane via a C-terminal polybasic motif and interacts with calcium channel beta

139 ontributions of its two distinct elements, a polybasic motif and palmitoylated cysteines, which when

140 ffector that directly binds PIP(2) through a polybasic motif and PIP(2) binding activates IQGAP1, fac

141 We identify a role for the lipin1 polybasic motif as both a lipid binding motif and a prim

142 id binding modules, such as PH domains, this polybasic motif binds PIP(2) in a multivalent, cooperati

143 a peptide aptamer, NaViPA1, carrying a short polybasic motif flanked by serine residues in a structur

144 We identify a polybasic motif in the proximal C terminus of retigabine

145 e used pharmacological agents and lipin1beta polybasic motif mutants to explore the role of PA-mediat

146 idification of the basic residues within the polybasic motif of KChIP2a1 rescued Ca(2+)-mediated regu

147 According to our models, this polybasic motif of the I-II linker forms a straight alph

148 /cytoplasmic shuttling and that a C-terminal polybasic motif proximal to the palmitoylation acceptor

149 oop identified several unique residues and a polybasic motif that contribute to the catalytic activit

150 his study, we demonstrate that the conserved polybasic motif that dictates the membrane topology and

151 his binding involves a previously identified polybasic motif that mediates activation of the enzyme b

152 Notably, N1 contains a polybasic motif that serves as a binding site for neurot

153 selectivity to function in concert with the polybasic motif to target the protein to PI(4,5)P2-rich

154 Studies using lipin1beta polybasic motif variants establish that this region is a

155 -targeting motifs (the prenyl anchor and the polybasic motif) are engaged by distinct lobes of CaM an

156 modeling to predict the conformation of this polybasic motif, immunofluorescence microscopy and live

157 ld can be tuned by varying the length of the polybasic motif.

158 and stimulation of catalysis mediated by the polybasic motif.

159 lations, and other techniques has shown that polybasic motifs and amphipathic helices are the main dr

160 Amphibian N-PrP, which contains the polybasic motifs but lacks a repeat domain and histidine

161 LLPS of mouse PrP is dependent on two polybasic motifs in N-PrP that are conserved in all tetr

162 We also found that polybasic motifs present in the cytoplasmic tails of CD4

163 sumably via cation-pai interactions with the polybasic motifs.

164 lytic domain in addition to the farnesyl and polybasic motifs.

165 of plasma membrane localization of alpha(q) polybasic mutants by introduction of a site for myristoy

166 of tau interacts electrostatically with the polybasic N-terminal intrinsically disordered segment of

167 Furthermore, the deletion of the short polybasic N-terminal region 23-30 was found to reduce th

168 A peptide containing the prototypical polybasic NLS sequence of the SV40 large T-antigen was a

169 alization of IFN-gamma is driven by a simple polybasic nuclear localization sequence (NLS) in its COO

170 ound that PIP(2) bound to the well conserved polybasic patch of the C2B domain with an apparent disso

171 interactions, we show that both C2A and C2B polybasic patches contribute to membrane binding, and bo

172 Electrostatic interactions between the NLRP3 polybasic (PB) region and negatively charged lipids on t

173 in variant that is linked to a farnesylated, polybasic peptide corresponding to the K-Ras4B C terminu

174 Our results show that this polybasic peptide partitions into the membrane interfaci

175 farnesyl cysteine methyl ester adjacent to a polybasic peptide segment, to the cytosolic face of the

176 Small polybasic peptides derived from the transduction domains

177 it has been proposed that certain endogenous polybasic PKC substrates may activate PKC in cells by th

178 Here we identified a polybasic plasma membrane binding motif, consisting of f

179 A mutant STIM1 lacking the C-terminal polybasic PM-targeting motif oligomerized after Ca(2+) s

180 we show that, similar to recently discovered polybasic polarity proteins such as Lgl and aPKC, Dlg al

181 s the electrostatic PM targeting of multiple polybasic polarity proteins.

182 nslocation is reversible and mediated by the polybasic-prenyl membrane targeting motif of KRas.

183 We conclude that the polybasic-prenyl motif acts as a Ca2+/CaM-regulated mole

184 location occurs through sequestration of the polybasic-prenyl motif by Ca2+/calmodulin (Ca2+/CaM) and

185 min-1-SNARE complex binding modes involving 'polybasic', 'primary' and 'tripartite' interfaces of syn

186 nity, consistent with a potential role for a polybasic protein or protein domain in the activation of

187 dent substrate protamine sulfate, which is a polybasic protein that activates PKC by a novel mechanis

188 of PKC isozymes at subcellular sites rich in polybasic proteins, it has been proposed that certain en

189 uding electrostatic PM targeting of numerous polybasic proteins.

190 These substrates share a bipartite polybasic recognition determinant (BPR) flanking a Cdk1

191 nd Rho small GTPases possessing a C-terminal polybasic region (PBR) are vital signaling proteins whos

192 One helix contains a polybasic region (PBR) composed of Arg-164, Arg-168, Lys

193 A polybasic region (PBR) in the C terminus blocks interact

194 The COOH-terminal polybasic region (PBR) of Rac1, a Rho family GTPase memb

195 GPCRs) phosphorylates S-179 and S-180 in the polybasic region (PBR) of Rap1B, which inhibits Rap1B bi

196 In this study, we identified a small polybasic region (PBR) preceding the RhoGAP domain that

197 tly identified residues within the ST8Sia-IV polybasic region (PBR) that are required for neural cell

198 nge by small GTPases containing a C-terminal polybasic region (PBR), such as Rac1 and RhoA.

199 ver, our studies suggest that the N-terminal polybasic region 23-30 is essential for effective foldin

200 afficking of GTPases containing a C-terminal polybasic region and demonstrated that SmgGDS-607 intera

201 , Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids

202 SmgGDS-607 recognizes the polybasic region and the CAAX box of several small GTPas

203 by an electrostatic interaction between its polybasic region and the endosomal acidic phospholipids,

204 ytically cleaves EC-SOD in the middle of the polybasic region and then requires an additional carboxy

205 phosphorylates K-Ras4B on serine 181 in the polybasic region and thereby induces translocation from

206 olycysteine sequence along with the adjacent polybasic region are both important for G16alpha-mediate

207 These findings establish the Pf1 polybasic region as a phosphoinositide-binding module an

208 hat mice expressing PrP deleted for a short, polybasic region at the N terminus (residues 23-31) disp

209 A polybasic region at the Rga6 C-terminus is responsible f

210 Furthermore, this polybasic region binds specifically to PI(3)P when fused

211 sulfatide headgroup, whereas the C-terminal polybasic region contributes to interactions with acyl c

212 We show that a triproline N-terminal to the polybasic region contributes to the NLS, which is crypti

213 nction analyses indicate that the C-terminal polybasic region contributes to tomosyn's inhibitory fun

214 The presence of an upstream polybasic region does not significantly affect GGTase I-

215 Thus, the presence of an upstream polybasic region enhances the dual prenylation of these

216 rast to known GEFs requires RhoA to retain a polybasic region for activation.

217 inal segment of B-Raf and requires the K-Ras polybasic region for high-affinity binding.

218 nase, one of the effectors that requires the polybasic region for interaction, is necessary for effic

219 Here we identify a polybasic region in Gic2 adjacent to the Cdc42/Rac inter

220 imeric G protein subunit alpha(q) contains a polybasic region in its N terminus that contributes to p

221 A polybasic region in the carboxyl terminus distinguishes

222 art of the plasma membrane binding signal, a polybasic region in the matrix protein, was mutated.

223 s the octapeptide repeats and amino-terminal polybasic region in the prion protein, but not its endoc

224 ulatory protein N-WASP by binding to a short polybasic region involved in N-WASP autoinhibition.

225 r demonstrated that the conserved C-terminal polybasic region is important for specific phosphoinosit

226 Previous work suggested that a polybasic region located prior to the conserved polysial

227 Here, the role of the N-terminal polybasic region of alpha(q) in signaling was addressed.

228 (2+)-calmodulin and F-actin; (c) show that a polybasic region of DAKAP200 is a substrate for protein

229 We show that the polybasic region of Rsr1 is necessary for the efficient

230 and Nox4 interactions were dependent on the polybasic region of the B-loop.

231 utative Syt1-SNARE/PIP2 coupling through the polybasic region of the C2B domain is critical for vesic

232 The polybasic region of the Rac1 C terminus functions both a

233 Specific effects of mutations in the polybasic region on Ca(2+)-dependent synaptotagmin-1-PIP

234 , our results indicate that the ST8SiaIV/PST polybasic region plays a critical role in substrate reco

235 by protein kinase C (PKC) of S181 within the polybasic region promotes rapid dissociation of K-Ras fr

236 Rac1(PBRM), a mutant lacking the C-terminal polybasic region required for Rac1 association with the

237 Replacing two polybasic region residues, Arg(82) and Arg(93), eliminat

238 disruption of the primary interface and the polybasic region show negligible impact on the modulator

239 cids in this conserved polysialyltransferase polybasic region that are critical for the protein-speci

240 The second is a 35-amino acid polybasic region that contains seven basic residues and

241 Surprisingly, a polybasic region that follows the PHD1 is necessary for

242 strates of these enzymes contain an upstream polybasic region that is proposed to increase the affini

243 high propensity for trimer formation, and a polybasic region that precedes the C-terminal prenylatio

244 demonstrate that the addition of an upstream polybasic region to a peptide substrate enhances the bin

245 ne residue at position 186 in the C-terminal polybasic region were found to possess a self-stimulator

246 on of ING2 (consisting of a PHD finger and a polybasic region) revealed a number of complementary sur

247 ctive ST8SiaIV/PST proteins that include the polybasic region, but not those that lack this region, c

248 B-loop, which in Nox1-4 contains a conserved polybasic region.

249 ma membranes by a farnesyl lipid group and a polybasic region.

250 ite of palmitoylation as well as a bipartite polybasic region.

251 l sequences (K(K/R)X(K/R)) in the C-terminal polybasic regions (PBRs) of some Rac and Rho isoforms.

252 gnaling triggered by the interaction between polybasic regions and phosphoinositides.

253 Polybasic regions are often associated with nonspecific

254 By exchanging the polybasic regions between different PHD fingers we show

255 embrane interactions, we show that conserved polybasic regions in both domains contribute to membrane

256 , and thereby facilitating interactions with polybasic regions of proteins.

257 f ST8Sia IV sequences revealed two conserved polybasic regions that might interact with the NCAM acid

258 g; individual cysteine residues, but not the polybasic regions, determine lipid modification and subc

259 inding motifs represented by two consecutive polybasic regions.

260 rating NADPH oxidase, contains two conserved polybasic regions: one N-terminal (PBR-N), located betwe

261 Both the polybasic residues and the adjacent prenylation motif ar

262 Deletion or mutation of the polybasic residues drastically decreased its intrinsic G

263 phospholipid PI(3,4,5)P3 and the stretch of polybasic residues preceding the RhoGAP domain regulates

264 h the sequence of the classical NLS contains polybasic residues that are recognized by importin-alpha

265 These mRNAs encode a stretch of polybasic residues that cause ribosome stalling.

266 Polybasic secretagogues such as mastoparan, compound 48/

267 inker interactions occlude the action of the polybasic segment and that its functional availability i

268 Here, we identified a short polybasic segment at the boundary of the guanylate kinas

269 The linker upstream from the polybasic segment, but not the N- and C-terminal variabl

270 on the plasma membrane, mediated through its polybasic sequence (185)KRK(187), which is essential for

271 IFN-gamma (IFN-gamma(95-132)) containing the polybasic sequence 126RKRKRSR132 was capable of specifyi

272 ich the proline-rich region was fused to the polybasic sequence from the HIV Tat protein to facilitat

273 The polybasic sequence in the C terminus of RhoA is essentia

274 Notably, the presence of a polybasic sequence in the PSGL-1 cytoplasmic domain sign

275 to cell-cell contacts and that an N-terminal polybasic sequence is necessary for PAK1 recruitment to

276 alpha, and G16alpha) in particular utilize a polybasic sequence of amino acids in their N terminus to

277 Mutations in a polybasic sequence on the side of the C2B domain beta-sa

278 -GG, which contains a prenylation site and a polybasic sequence similar to K-ras.

279 GEF-independent mechanism requires the Rho1 polybasic sequence that binds to acidic phospholipids, i

280 The contaminants bind to a polybasic sequence that has been previously implicated i

281 Nuclear import was abolished when the above polybasic sequence was deleted.

282 e IFN-gamma(95-125), which is deleted in the polybasic sequence, further confirming that the NLS prop

283 ng potential depends on the integrity of the polybasic sequence.

284 at functions in conjunction with an adjacent polybasic sequence.

285 residues where Rac1 but not Rac2 contains a polybasic sequence.

286 d CD44 with assembling HIV-1 which relies on polybasic sequences in HIV-1 Gag and in cytoplasmic doma

287 Translation arrest by polybasic sequences induces ribosome stalling, and the a

288 nuclear targeting is mediated in part by two polybasic sequences present at the C-terminal end of SKC

289 sociation with assembling HIV-1 Gag in which polybasic sequences present in the cytoplasmic tails of

290 with unknown function, both of which contain polybasic sequences.

291 o the binding of phosphatidate to a specific polybasic site within the kinase domain of Raf-1.

292 urring mutants that harbour deletions at the polybasic site.

293 cleavage at multiple specific extracellular polybasic sites, releasing inhibitory tracts from the ch

294 In this model, the polybasic strand of C2B forms the membrane binding surfa

295 mutation of a single basic residue within a polybasic stretch of the DEP domain, which abolishes tra

296 initiate chemical signaling, as well as the polybasic stretches that regulate signal potentiation.

297 positions in aged yeast and worms, including polybasic stretches, leading to increased ribosome colli

298 luenza A virus infections, to both mono- and polybasic subtypes.

299 d CD3epsilon tails, which are disordered and polybasic, suggested regulation of phosphorylation throu

300 Polybasic tract pairs evolved with the terrestrial migra