ライフサイエンスコーパス: polynucleotide kinase

1 RCC4, known to be bound by the FHA domain of polynucleotide kinase.

2 end repair with Klenow, T4 polymerase or T4 polynucleotide kinase.

3 Mammalian polynucleotide kinase 3' phosphatase (PNK) plays a key r

4 r pathogenic ATXN3-expressing cells abrogate polynucleotide kinase 3'-phosphatase (PNKP) activity.

5 2 (NEIL2) and the DNA end-processing enzyme polynucleotide kinase 3'-phosphatase (PNKP) in purified

6 Mammalian polynucleotide kinase 3'-phosphatase (PNKP), a DNA end-p

7 Polynucleotide kinase 3'-phosphatase (PNKP), an essentia

8 in level, of an essential DNA repair enzyme, polynucleotide kinase 3'-phosphatase (PNKP), is severely

9 nt NHEJ is mediated by the DNA repair enzyme polynucleotide kinase 3'-phosphatase (PNKP), which shows

10 identification of the same homozygous PNKP (polynucleotide kinase 3'-phosphatase) mutation, c.1123G>

11 3 and identified multiple mutations in PNKP (polynucleotide kinase 3'-phosphatase) that result in sev

12 recruit end-processing enzymes, such as PNK (polynucleotide kinase 3'-phosphatase), Aprataxin and APL

13 ant for SSBR, including DNA ligase 3 (DNL3), polynucleotide kinase 3'-phosphatase, and polymerase bet

14 A (POLR2A), ataxin-3, the DNA repair enzyme polynucleotide-kinase-3'-phosphatase (PNKP), and cyclic

15 ccharomyces cerevisiae, a homologue of human polynucleotide kinase/3'-phosphatase, has been shown to

16 It is homologous to one domain of mammalian polynucleotide kinase/3'-phosphatase.

17 xosome), which contains RNA endonuclease and polynucleotide kinase activities with known roles in rib

18 11 and His515 in the kinase module abolished polynucleotide kinase activity in vitro.

19 n used this motif to engineer ribozymes with polynucleotide kinase activity.

20 RNA conjugate is labeled using the enzyme T4 polynucleotide kinase and a 1:1 mixture of heavy (18)O(4

21 Polynucleotide kinase and aprataxin-like forkhead-associ

22 le-stranded cDNA (ss-cDNA) ligation using T4 polynucleotide kinase and CircLigase, and polymerization

23 They were substrates for T4 polynucleotide kinase and primers for Escherichia coli p

24 g 3'-blocked ends of BER intermediates, e.g. polynucleotide kinase and tyrosyl-DNA phosphodiesterase

25 ligase 1, a central domain that resembles T4 polynucleotide kinase, and a C-terminal CPD domain that

26 agment is phosphorylated by an NTP-dependent polynucleotide kinase; and (iii) the 3'-OH, 2'-PO4, and

27 ragment is phosphorylated by a GTP-dependent polynucleotide kinase; and the 3'-OH, 2'-PO(4), and 5'-P

28 EN complex additionally co-purifies with the polynucleotide kinase CLP1; however, CLP1's role in tRNA

29 Clostridium thermocellum polynucleotide kinase (CthPnk), the 5' end-healing modul

30 Clostridium thermocellum polynucleotide kinase (CthPnk), the 5'-end-healing modul

31 erminal cyclic phosphodiesterase and central polynucleotide kinase domains that heal the broken ends

32 bonuclease requires its binding partner, the polynucleotide kinase Grc3, for specific C2 cleavage.

33 g a phosphorylated primer, prepared using T4 polynucleotide kinase, into the PCR phase and subsequent

34 Polynucleotide kinase is a bifunctional enzyme containin

35 a homologue of the 3' phosphatase domain of polynucleotide kinase, is a third member of this group o

36 an N-terminal ligase (LIG) domain; a central polynucleotide kinase (KIN) domain; and a C-terminal cyc

37 hodiesterase (CPD) and central GTP-dependent polynucleotide kinase (KIN) domains that heal the broken

38 Nearly 50 individual DNAs with polynucleotide kinase-like activity were isolated from a

39 ant, A482T, that was defective in binding to polynucleotide kinase phosphatase (PNKP) not only retain

40 er requires APE1 but instead is dependent on polynucleotide kinase phosphatase (PNKP1) activity.

41 plementing group 1 (XRCC1) and aprataxin and polynucleotide kinase phosphatase-like factor (APLF).

42 Clostridium thermocellum polynucleotide kinase-phosphatase (CthPnkp) catalyzes 5'

43 T4 polynucleotide kinase-phosphatase (Pnkp) exemplifies a f

44 Polynucleotide Kinase-Phosphatase (PNKP) is a bifunction

45 Polynucleotide kinase-phosphatase (PNKP) is a DNA repair

46 Here, we reveal that polynucleotide kinase-phosphatase (PNKP) is another key

47 Hereditary mutations in polynucleotide kinase-phosphatase (PNKP) result in a spe

48 ncludes the DNA damage end processing enzyme polynucleotide kinase-phosphatase (PNKP), and that PIAS1

49 for cell killing in vivo, and the bacterial polynucleotide kinase-phosphatase (Pnkp)/hua enhancer 1

50 Here, we demonstrate that a polynucleotide kinase-phosphatase enzyme from Clostridiu

51 ponent RNA repair cassette, comprising Pnkp (polynucleotide kinase-phosphatase-ligase) and Hen1 (RNA

52 osphatase domain of Clostridium thermocellum polynucleotide kinase/phosphatase (CthPnkp) belongs to t

53 egB-cleaved mRNAs depends on a functional T4 polynucleotide kinase/phosphatase (PNK).

54 Polynucleotide kinase/phosphatase (PNKP) and X-ray repai

55 T4 polynucleotide kinase/phosphatase (Pnkp) exemplifies a f

56 a hereditary disease caused by mutations in polynucleotide kinase/phosphatase (PNKP), a DNA strand b

57 important interactions of XRCC1 is that with polynucleotide kinase/phosphatase (PNKP), a dual-functio

58 NHEJ relies on polynucleotide kinase/phosphatase (PNKP), which generate

59 tic lethal partner of the DNA repair protein polynucleotide kinase/phosphatase (PNKP).

60 he bacteriophage T4 enzymes RNA ligase 1 and polynucleotide kinase/phosphatase can fulfill the tRNA a

61 Aprataxin polynucleotide kinase/phosphatase-like factor (APLF) fac

62 with accessory factors such as aprataxin and polynucleotide kinase/phosphatase-like factor (APLF).

63 ling activities and requires the action of a polynucleotide kinase (PNK) and a cyclic phosphodiestera

64 was efficiently religated in the presence of polynucleotide kinase (PNK) and human DNA ligase III (Li

65 T4 phage polynucleotide kinase (PNK) displays 5'-hydroxyl kinase,

66 The endoribonuclease (RNase) Las1 and the polynucleotide kinase (PNK) Grc3 assemble into a multien

67 T4 polynucleotide kinase (Pnk) is a bifunctional 5'-kinase/

68 T4 polynucleotide kinase (Pnk) is the founding member of a

69 T4 polynucleotide kinase (PNK) plays critical roles in regu

70 y catalyse NHEJ in vitro, we show that human polynucleotide kinase (PNK) promotes phosphate replaceme

71 removal of the 3' phosphate is dependent on polynucleotide kinase (PNK), and not APE.

72 e dual function mammalian DNA repair enzyme, polynucleotide kinase (PNK), facilitates strand break re

73 T4 polynucleotide kinase (Pnk), in addition to being an inv

74 thophosphate from [gamma-32P]ATP mediated by polynucleotide kinase (PNK); (iv) chromatographic or ele

75 phorylated product produced by reaction with polynucleotide kinase, potentially indicating simultaneo

76 to trnfM; the termini could be labeled with polynucleotide kinase, suggesting that they result from

77 The new assays described herein are for T4 polynucleotide kinase, the DNA repair enzymes uracil-DNA

78 1L endoribonuclease associates with the Nol9 polynucleotide kinase to form the internal transcribed s

79 The fragments could be relabeled with polynucleotide kinase to yield highly purified, high-spe

80 (here named TPP1) is shown to encode only a polynucleotide kinase-type 3'-phosphatase.

81 T4-polynucleotide kinase was used to enzymatically substitu

82 Label, incorporated using [gamma-32P]ATP and polynucleotide kinase, was increased in proportion to th

83 NA-seq") incorporating RNA treatment with T4-polynucleotide kinase, which we compared with standard s