ライフサイエンスコーパス: polysialic acid

1 omote neurite outgrowth similarly to natural polysialic acid.

2 , which expresses long-chain a(2-->8)-linked polysialic acid.

3 f N-glycans substituted with short chains of polysialic acid.

4 ion, which is released upon interaction with polysialic acid.

5 cking to secondary lymphatic organs, carries polysialic acid.

6 scription 3; these processes were blocked by polysialic acid.

7 nctionally essential effector domain (ED) to polysialic acid.

8 te (MARCKS) is an intracellular receptor for polysialic acid.

9 sferase catalyzed the synthesis of alpha-2,8-polysialic acid.

10 orted G2(+) left-handed helix of alpha-(2,8)-polysialic acid.

11 eased in vivo and in vitro in the absence of polysialic acid.

12 g and distinguishing between different oligo/polysialic acids.

13 antibody response against alpha-(2,8)-linked polysialic acids.

14 hesis and catabolism of microbial sialic and polysialic acids.

15 Polysialic acid, a homopolymer of alpha2,8-linked sialic

16 the transfer of neuNAc from CMP-neuNAc to a polysialic acid acceptor is catalyzed by a complex with

17 ive fashion when initiated with an alpha-2,8-polysialic acid acceptor.

18 to be mediated at least in part by increased polysialic acid addition to neural cell adhesion molecul

19 Loss of polysialic acid affected both tangential and radial migr

20 These results indicate that polysialic acid and mucin type O-glycans on NCAM differe

21 eningitidis serogroup B, (alpha2-->8)-linked polysialic acid and the capsules of other meningococcal

22 an rhabdomyosarcoma cells, which overexpress polysialic acid, and C2C12 cells.

23 , isogenic serogroups B [(alpha2-->8)-linked polysialic acid] and C [(alpha2-->9)-linked polysialic a

24 Anti-oligo/polysialic acid antibodies have DP-dependent antigenic s

25 Naturally occurring polysialic acids are not viable candidates because they

26 erase that catalyzes the formation alpha-2,9-polysialic acid as a soluble enzyme.

27 d from CMP-sialic acid to a growing chain of polysialic acid at the nonreducing end.

28 Polysialic acid attached to the neural cell adhesion mol

29 sis of mutants that accumulate intracellular polysialic acid because of export defects (kpsM and kpsS

30 nt virulence factor in these diseases is the polysialic acid capsular polysaccharide (K1 antigen), wh

31 is group C are based on its alpha-2,9-linked polysialic acid capsular polysaccharide.

32 pid attached to the reducing terminus of the polysialic acid capsular polysaccharides from E. coli K1

33 ans and domestic animals; in this strain the polysialic acid capsule (K1 antigen) functions by inhibi

34 ecific pathogen Escherichia coli K1 uses its polysialic acid capsule as a molecular mimic to engage S

35 s confirm the intrinsic relationship between polysialic acid capsule biosynthesis and lipooligosaccha

36 involved in CMP-N-acetylneuraminic acid and polysialic acid capsule biosynthesis, and in ctrA the fi

37 kpsM, allowing the expression of an alpha2,8 polysialic acid capsule in E. coli.

38 esults indicate that the (alpha2-->8)-linked polysialic acid capsule modifies the interaction of meni

39 ns 7 or 9 of the sialic acid residues in the polysialic acid capsule of Escherichia coli K1 is cataly

40 utilizes ATP to effect translocation of the polysialic acid capsule of Escherichia coli K1.

41 es, the influence of the alpha2-->8) -linked polysialic acid capsule on the interaction of N. meningi

42 Opa, Opc, glycolipid GgO4-binding adhesins, polysialic acid capsule or a particular lipooligosacchar

43 herichia coli K1 synthesizes and assembles a polysialic acid capsule virulence factor on the external

44 ene cluster required for the biosynthesis of polysialic acid capsule was mapped to a location immedia

45 rom an isogenic bacterial mutant lacking the polysialic acid capsule.

46 lation or acetylation of the alpha2-9-linked polysialic acid capsule.

47 The removal of polysialic acid caused several distinct abnormalities, i

48 ains its apparent high affinity for a longer polysialic acid chain by recognizing every three sialic

49 c groove on the polyST surface that promotes polysialic acid chain polymerization are identified, and

50 ct with the NCAM acidic patch or the growing polysialic acid chain.

51 he unit catalyzing the extension of existing polysialic acid chains does not differ significantly fro

52 ively non-immunogenic molecular mimic of the polysialic acid chains found in high concentrations on n

53 enzyme itself is modified by alpha2,8-linked polysialic acid chains in vivo.

54 erases bind to the FN1 of NCAM to polymerize polysialic acid chains on appropriately presented glycan

55 However, elongation of preexisting polysialic acid chains only requires expression of neuS.

56 opical methods demonstrated that full-length polysialic acid chains were synthesized but not exported

57 ed polysialyltransferases suggest that their polysialic acid chains, like those of NCAM, may modulate

58 f the individual sialic acid residues of the polysialic acid chains.

59 The virulent E. coli strain had a polysialic acid-containing capsule, whereas the nonvirul

60 glycosphingolipids characterized by mono- or polysialic acid-containing oligosaccharides linked throu

61 lysialyltransferase, NeuS, cannot synthesize polysialic acid de novo without other products of the ge

62 ng the entire K1 gene cluster can synthesize polysialic acid de novo.

63 Consistent with these findings, polysialic acid-deficient mice exhibited increased expre

64 s a unique preference for longer polymers of polysialic acid (DP >10), yet the mechanism of recogniti

65 tial targeting of polysialyltransferases and polysialic acid engineering are promising strategies to

66 Polysialic acid exhibits a highly regulated expression p

67 t least four of these genes are required for polysialic acid export.

68 This polysialic acid expressed on the surface of N. meningiti

69 xamined how unnatural sialic acids can alter polysialic acid expression and influence the adhesive pr

70 In a previous study, we showed that polysialic acid facilitates astrocytic tumor invasion an

71 Some SEZ cells expressed the polysialic acid form of neural cell adhesion molecule (P

72 Here, we describe an analysis of capsular polysialic acid form variation in Escherichia coli K1, d

73 oducts required for de novo synthesis of the polysialic acid from CMP-NeuNAc in K1 E. coli.

74 ase recognition, but shifted the addition of polysialic acid from the N-glycans modifying the adjacen

75 Removal of polysialic acid from the surface of dendritic cells or b

76 ed using chemically modified N-propionylated polysialic acid, from Escherichia coli K1 polysaccharide

77 It is currently unclear how polysialic acid functions in different processes of neur

78 Polysialic acid has been detected in multiple sclerosis

79 it can form high-molecular-weight alpha-2,9-polysialic acid in a nonprocessive fashion when initiate

80 By contrast, forced expression of polysialic acid in early differentiation stages reduces

81 of mouse adult brain, however, suggests that polysialic acid in the hippocampal formation is synthesi

82 and STX are polysialyltransferases that form polysialic acid in the neural cell adhesion molecule (N-

83 and STX are polysialyltransferases that form polysialic acid in the neural cell adhesion molecule (NC

84 t sufficient to support de novo synthesis of polysialic acid in vitro.

85 ested in developing structural surrogates of polysialic acids in an effort to overcome these limitati

86 Polysialic acid is a developmentally regulated, anti-adh

87 Polysialic acid is a developmentally regulated, anti-adh

88 Polysialic acid is a glycan modification of the neural c

89 Polysialic acid is a linear homopolymer of alpha2-8-link

90 Polysialic acid is a polymer of alpha2,8-linked sialic a

91 Polysialic acid is a unique carbohydrate polymer specifi

92 Polysialic acid is an anti-adhesive glycan that modifies

93 Polysialic acid is an anti-adhesive protein modification

94 Polysialic acid is an oncofetal glycopolymer, added to t

95 accharides and that the vast majority of the polysialic acid is found on the oligosaccharide modifyin

96 Polysialic acid is primarily attached to N-glycans of NC

97 of the Escherichia coli K1 group 2 capsular polysialic acid (K1 antigen) occur within a protected su

98 post-translational modification of NCAM-1 by polysialic acid leads to disrupted trafficking of sarcol

99 , we show that Gata1-KO(DC) DCs have reduced polysialic acid levels on their surface, which is a know

100 polysialic acid] and C [(alpha2-->9)-linked polysialic acid] meningococcal isolates from an outbreak

101 Measurements with the polysialic-acid-modified form of NCAM reveal that, at ph

102 we demonstrate that selectively cleaving the polysialic acid moiety, using the bacteriophage-derived

103 We identified the polysialic acid molecule (PSA) as a mediator of the diet

104 abile glycans such as heavily sialylated and polysialic acid N-glycans, which are difficult to detect

105 ursor (NRP) cell that expresses E-NCAM (high polysialic-acid NCAM) and is morphologically distinct fr

106 Polysialic acid negatively regulates cell adhesion, is r

107 erotype are encapsulated with the alpha(2-8)-polysialic acid NeuNAc(alpha2-8), common to several bact

108 tochemistry for bromodeoxyuridine (BrdU) and polysialic acid-neural cell adhesion molecule (PSA-NCAM)

109 The up- and downregulation of polysialic acid-neural cell adhesion molecule (PSA-NCAM)

110 s, stathmin immunoreactivity was observed in polysialic acid-neural cell adhesion molecule-positive m

111 alyltransferase (PST) that forms the group C polysialic acid (NmC PST) is located in the cytoplasmic

112 al importance of O-acetylation, no sialic or polysialic acid O-acetyltransferase has been identified

113 es of di- and trisaccharide fragments of the polysialic acid O-antigen capsular polysaccharide (CPS)

114 ST8SiaIV to determine the effects of loss of polysialic acid on brain development.

115 The presence of polysialic acid on NCAM has been shown to modulate cell-

116 of PST and STX more efficiently synthesized polysialic acid on NCAM than PST or STX alone.

117 hich these two enzymes form large amounts of polysialic acid on NCAM were heretofore unknown.

118 the normal expression patterns of EphA4 and polysialic acid on NCAM, which may contribute to the pat

119 he sialyltransferase gene family, synthesize polysialic acid on NCAM.

120 ptors but differ in the efficiency of adding polysialic acid on NCAM.

121 PST and STX were found to add polysialic acid on NCAM.Fc molecules sialylated by alpha

122 ormal expression levels of EphA4, EphB1, and polysialic acid on neural cell adhesion molecule, three

123 ase (STX) gene expression and an increase in polysialic acid on neural cell adhesion molecule.

124 First, we found that ST8Sia III can form polysialic acid on the enzyme itself (autopolysialylatio

125 Polysialic acid on the neural cell adhesion molecule (NC

126 for complex N-glycans showed the presence of polysialic acid on the neural cell adhesion molecule.

127 a II, and ST8Sia III all add oligosialic and polysialic acid on various sialylated N-acetyllactosamin

128 utional, and functional diversities of oligo/polysialic acids (OSA/PSA) that exist in organisms rangi

129 Cell surface polysialic acid plays roles in cell adhesion and differe

130 t nucleus of the solitary tract has abundant polysialic acid (polySia) and is a major site of integra

131 STATEMENT The beneficial or adverse role of polysialic acid (polySia) in myelin repair is a long-sta

132 Polysialic acid (polySia) is a large glycan polymer that

133 Polysialic acid (polySia) is a large glycan with restric

134 Polysialic acid (polySia) is known for its important rol

135 anNBut) with a goal to achieve modulation of polysialic acid (polySia) on neural cell adhesion molecu

136 and co-workers demonstrated the presence of polysialic acid (polySia) on sea urchin sperm.

137 Polysialic acid (polySia), a homopolymer of alpha2,8-lin

138 This linkage is prominent in polysialic acid (polySia), a molecule with critical role

139 such target is the oncodevelopmental antigen polysialic acid (polySia), a polymer of alpha2,8-linked

140 econdary human lymphoid organs, its product, polysialic acid (polySia), has been largely overlooked b

141 xpression of the large extracellular glycan, polysialic acid (polySia), is restricted in the adult, t

142 ar homo-polymers, with its most complex form polysialic acid (polySia).

143 1, a function that relies on the presence of polysialic acid (polySia).

144 , and other distinct glycan features such as polysialic acids (PolySia), sulfation, and proteoglycan

145 t encode transport proteins (yihN and yihP), polysialic acid production (gutM and gutQ), CP4-57 proph

146 Polysialic acid (PSA) and its major protein carrier, the

147 sent study was to evaluate the expression of polysialic acid (PSA) and the cell adhesion molecule L1

148 membrane-membrane apposition based solely on polysialic acid (PSA) biophysical properties.

149 Polysialic acid (PSA) capsules are cell-associated homop

150 A role for the polysialic acid (PSA) component of PSA-NCAM, which is kn

151 Enzymatic removal of polysialic acid (PSA) from nerve and muscle during norma

152 Polysialic acid (PSA) fulfills several criteria for a mo

153 alpha-2,8-linked form of the polysaccharide polysialic acid (PSA) has widespread implications in phy

154 aspects of human biology, the expression of polysialic acid (PSA) in human tissues is thought to be

155 The highly negatively charged polysialic acid (PSA) is a carbohydrate predominantly ca

156 Polysialic acid (PSA) is a developmentally regulated car

157 Polysialic acid (PSA) is a homopolymeric glycan that pla

158 Polysialic acid (PSA) is a large negatively charged glyc

159 Polysialic acid (PSA) is a linear homopolymer of alpha-2

160 Polysialic acid (PSA) is a post-translational protein mo

161 Polysialic acid (PSA) is a unique linear homopolymer of

162 In vertebrates, polysialic acid (PSA) is typically added to the neural c

163 The polysialic acid (PSA) modification of the neural cell ad

164 The polysialic acid (PSA) moiety of NCAM can serve as a nega

165 The polysialic acid (PSA) moiety of the neural cell adhesion

166 to be reflected most sensitively in reduced polysialic acid (PSA) on neural cell adhesion molecules.

167 the repulsion also depends on the amount of polysialic acid (PSA) on the membranes, and the PSA-depe

168 developmentally regulated marker 2,8-linked polysialic acid (PSA) regulate viral transport and tropi

169 anoylmannosamine are effective inhibitors of polysialic acid (PSA) synthesis in stably transfected He

170 ot appear to alter sensory projections, when polysialic acid (PSA) was enzymatically removed from NCA

171 sion molecule (NCAM) is the major carrier of polysialic acid (PSA) which modulates NCAM functions of

172 of the spinal cord expresses high levels of polysialic acid (PSA), a cell surface carbohydrate known

173 Polysialic acid (PSA), a homopolymer attached to the neu

174 Polysialic acid (PSA), a large cell-surface carbohydrate

175 e is chemically identical to an autoantigen, polysialic acid (PSA), and is a poor immunogen, even whe

176 B and of Escherichia coli K1, alpha(2 --> 8) polysialic acid (PSA), is unusual, because when injected

177 Here we show that polysialic acid (PSA), presented by the neural cell adhe

178 The present study used polysialic acid (PSA)-deficient and NCAM mutant mice to

179 f the NCAM-180 isoform that in brain carries polysialic acid (PSA).

180 , and that this defect reflects loss of NCAM polysialic acid (PSA).

181 he predominant carrier of the unusual glycan polysialic acid (PSA).

182 nmodified MBPS or chemically identical human polysialic acid (PSA).

183 i K1 [which, like NMGB, is alpha(2-8)-linked polysialic acid (PSA)] and bound to EV36, a nonpathogeni

184 d with antibodies to N-CAM and antibodies to polysialic acid (PSA-N-CAM), which is present on N-CAM a

185 Complex polysialic acids (PSAs) exist naturally and provide a me

186 These results indicate that polysialic acid regulates cell migration and differentia

187 nt results suggest that de novo synthesis of polysialic acid requires coexpression of four genes from

188 th vomeronasal nerves (VNN) that express the polysialic acid-rich form of the neural cell adhesion mo

189 es strongly suggest that the N-propionylated polysialic acid-rPorB conjugate is an excellent vaccine

190 Expression of the (alpha2-->8)-linked polysialic acid serogroup B capsule was essential for me

191 The relaxivity of the gadolinium polysialic acid species formed in vitro was 97.8 mM/sec

192 ole-mount tecta of embryos pretreated with a polysialic acid-specific degrading enzyme, endoneuramini

193 tform for the rational design of alpha-(2,8)-polysialic acid surrogates.

194 Strikingly, inhibition of polysialic acid synthesis by antisense DNA approach indu

195 rization of full-length proteins involved in polysialic acid synthesis in E. coli K1, as well as hete

196 Sia II and ST8Sia IV, play dominant roles in polysialic acid synthesis on NCAM.

197 ividual and combined roles of PST and STX in polysialic acid synthesis, in the present study we asked

198 However, polysialic acid synthesized by PST appears to be a bette

199 The results also demonstrated that polysialic acid synthesized by PST is larger than that s

200 . coli strain having abundant outer membrane polysialic acid, targeted deletion of the polysialyltran

201 N-glycans containing more, and thus longer, polysialic acid than when the enzymes were used individu

202 hic molecule and can be elongated to produce polysialic acid that is reactive with group C-specific a

203 immune response against alpha(2-->8)-linked polysialic acid, the capsular polysaccharide of Group B

204 and concurrent translocation of the capsular polysialic acid through sites of inner and outer membran

205 d, neither ST8Sia II nor ST8Sia IV could add polysialic acid to a polysialylated antenna of NCAM N-gl

206 and replacing the alpha-helix or QVQ shifts polysialic acid to FN1 O-glycans in full-length NCAM.

207 SA-NCAM show heterophilic adhesion involving polysialic acid to heparan sulfate proteoglycan and agri

208 The addition of polysialic acid to N- and/or O-linked glycans, referred

209 aused by: 1) the ability of ST8Sia IV to add polysialic acid to oligosialic acid formed by ST8Sia II,

210 Furthermore, ST8Sia IV was able to add polysialic acid to oligosialylated oligosaccharides and

211 findings indicate that the unique ability of polysialic acid to regulate different types of cell inte

212 on in brain microglia and engages endogenous polysialic acid to suppress inflammation.

213 The addition of alpha2,8-polysialic acid to the N-glycans of the neural cell adhe

214 By contrast, ST8Sia II added little polysialic acid to the same acceptors.

215 region whose gene products are required for polysialic acid transport and because capsule production

216 e kps locus for biosynthesis of the capsular polysialic acid virulence factor in Escherichia coli K1

217 On this basis, the role of polysialic acid was analyzed with respect to both trajec

218 nas in N-glycans attached to NCAM, even when polysialic acid was attached to at least one of the othe

219 We found that larger polysialic acid was formed on the enzymes themselves (au

220 Polysialic acid was formed well on Lec4 and Lec13 cells,

221 Because so few proteins carry polysialic acid, we hypothesized that polysialylation is

222 nd gadolinium trichloride in the presence of polysialic acid were also performed.

223 Polysialic acid, which is synthesized by two polysialylt

224 ct of E. coli K92 catalyzes the synthesis of polysialic acid with alpha2,9- and alpha2,8-linkages in

225 number of mammalian proteins are modified by polysialic acid, with the neural cell adhesion molecule