ライフサイエンスコーパス: product inhibition

1 ing active-site accessibility and preventing product inhibition.

2 f Gbetagamma is that it relieves competitive product inhibition.

3 the destruction of AdoHcy, thus alleviating product inhibition.

4 tion slows down at higher conversions due to product inhibition.

5 uding "approach to equilibrium" kinetics and product inhibition.

6 ltransferases undergo marked AdoHcy-mediated product inhibition.

7 His-30 and the evolutionary conservation of product inhibition.

8 active-site titration, product analysis, and product inhibition.

9 l phosphates that avoids the complication of product inhibition.

10 then promotes a competing pathway leading to product inhibition.

11 differential substrate turnover rather than product inhibition.

12 the biphasic kinetics are not the result of product inhibition.

13 ation of nicotinate mononucleotide and shows product inhibition.

14 small molecule production, and resistance to product inhibition.

15 ucose 6-phosphate, and phosphate reversal of product inhibition.

16 sms of IKKalpha and IKKbeta by substrate and product inhibition.

17 or Va and factor VaLEIDEN, and the effect of product inhibition.

18 m in which the zero-order phase results from product inhibition.

19 ype MnSOD, which can account for its lack of product inhibition.

20 luding unfavorable equilibrium positions and product inhibition.

21 ed in the regulation of these enzymes by CTP product inhibition.

22 he high affinity site associated with potent product inhibition.

23 ite, which suggested that Ape1 might exhibit product inhibition.

24 erevisiae is allosterically regulated by CTP product inhibition.

25 under physiological conditions and minimizes product inhibition.

26 anes enriched in PI(4,5)P2 and is subject to product inhibition.

27 s endpoint was shown to be the result of end product inhibition.

28 icating that the "burst" phase is not due to product inhibition.

29 eases, but was still >30%, due to reversible product inhibition.

30 phatase activity, where pyrophosphate causes product inhibition.

31 rease enzyme activity by relieving substrate/product inhibition.

32 Michaelis-Menten kinetics, with competitive product inhibition.

33 T1-KO cells with PC liposomes to restore end-product inhibition.

34 is by transition state stabilization without product inhibition.

35 d bacterial MnSODs, due to very low level of product inhibition.

36 roxide (O(2)(-)), due to different levels of product inhibition.

37 sis and has a significant role in regulating product inhibition.

38 tivities of these subunits are regulated via product inhibition.

39 chain conformations may affect the extent of product inhibition.

40 ategies for engineering cellulases to reduce product inhibition and enhance cellulose degradation, su

41 nalis PNP-catalyzed reactions, determined by product inhibition and equilibrium isotope exchange, was

42 ts at position 143 showed very low levels of product inhibition and favored Mn(II)SOD in the resting

43 g state, whereas the wild type showed strong product inhibition and favored Mn(III)SOD.

44 he kinetic mechanism of GFS as determined by product inhibition and fluorescence binding studies is c

45 tion of this dynamic fragment leads to lower product inhibition and highly stable glow-type biolumine

46 Mn-SOD, which exhibits significantly reduced product inhibition and increased enzymatic efficiency.

47 Product inhibition and isotope partitioning studies supp

48 perimental understanding of the mechanism of product inhibition and its role in the kinetics of the i

49 ls may use different mechanisms to alleviate product inhibition and modulate cholesteryl ester biosyn

50 nism under initial rate conditions; however, product inhibition and product accumulation led to PglH

51 other mechanisms sufficiently alleviate TDG product inhibition and promote its enzymatic turnover in

52 The use of template chaperone also overcomes product inhibition and renders the directing templates c

53 PafA has a much weaker product inhibition and slightly higher activity relative

54 Product inhibition and solvent isotope effects were also

55 The use of dead-end and product inhibition and solvent-isotope effect reveals th

56 e data suggest that GOAT is subjected to end-product inhibition and this inhibition is better achieve

57 required to curtail substrate saturation and product inhibition and to allow efficient catalysis.

58 produced unsaturated alcohol, which reduced product inhibition and undesired secondary reaction path

59 In order to investigate the mechanism of product inhibition and whether it is a feature common to

60 ts from non-linear kinetic time courses with product inhibition and/or substrate depletion.

61 tion, (2) enhancing yields by suppressing co-product inhibition, and (3) activating the azinium produ

62 gh catalytic proficiency, lack of observable product inhibition, and ability to hydrolyze both cocain

63 These include allosteric regulation, product inhibition, and covalent modification as well as

64 ed to perform steady-state initial velocity, product inhibition, and dead end inhibition experiments,

65 Initial velocity, product inhibition, and dead-end analog inhibition studi

66 ns were investigated using initial velocity, product inhibition, and dead-end inhibition analyses.

67 Initial velocity studies, product inhibition, and dead-end inhibition studies indi

68 Initial velocity, product inhibition, and dead-end inhibition studies indi

69 Initial velocity, product inhibition, and dead-end inhibition studies prov

70 Using a combination of initial velocity, product inhibition, and dead-end inhibition studies, the

71 Initial velocity, product inhibition, and deuterium kinetic isotope effect

72 A series of steady-state kinetic, product inhibition, and direct binding studies with Myco

73 Steady-state kinetics, product inhibition, and isotope exchange studies are con

74 This lowers product inhibition, and the target DNA or RNA thus becom

75 nd alcohol substrates, the aldehydes exhibit product inhibition, and we propose that this is due to n

76 ly, both the mt- and plPDCs are sensitive to product inhibition, and, potentially, to metabolite effe

77 lase activity, but the mechanisms underlying product inhibition are not clear.

78 udies, including kinetic isotope effects and product inhibition, are discussed in light of this kinet

79 y the 'strand separation problem', a form of product inhibition arising from the extraordinary stabil

80 The inefficient removal of product inhibition associated with ADP accounts for the

81 bstrate concentration because of the acetate product inhibition at each temperature.

82 e at the N-terminal half, the site of potent product inhibition at the C-terminal half, and a seconda

83 We describe an approach to study product inhibition at the single-molecule level.

84 catalytic dwell consistent with competitive product inhibition but also decreased the angular veloci

85 tetrahydrofolate synthase not only abolishes product inhibition but also increases the initial rate o

86 leotide and to elucidate the kinetic scheme, product inhibition by 8-oxo-dGMP and dGMP and direct bin

87 Product inhibition by adenine was noncompetitive against

88 tivity at pH 6.5 by preventing substrate and product inhibition by ATP and ADP, respectively.

89 aspects: (1) p50RhoGAP displays an effect of product inhibition by binding to the GDP-bound form of R

90 A simple kinetic model explains how product inhibition by CO(2) leads to overall first-order

91 ts, Bi-substrate kinetic analysis, authentic product inhibition by coenzyme A (CoA) and acetylated H3

92 the possibility of elucidating the origin of product inhibition by comparing human MnSOD with ScMnSOD

93 rylated CTP synthetase was less sensitive to product inhibition by CTP.

94 in a decrease in the enzyme's sensitivity to product inhibition by CTP.

95 uanosine at much higher K(m) values, and end-product inhibition by dCTP.

96 ctedly, our results also uncover significant product inhibition by deconjugated Cul1, which results f

97 can "activate" IDH phosphatase by reversing product inhibition by dephospho-IDH.

98 the metal stoichiometry at the active site, product inhibition by GDP, a potent competitive inhibito

99 6-P of 7 microm, and was highly sensitive to product inhibition by GlcN-6-P.

100 yldolichol (GlcNAc-GlcNAc-P-P-dolichol), and product inhibition by GlcNAc-P-P-dolichol itself.

101 ehydrogenase is also known to exhibit strong product inhibition by H4PteGlu5.

102 It alleviates product inhibition by L-Cth and revealed that the values

103 noyl-CoA, and dodecenoyl-CoA substrates, and product inhibition by lauroyl-CoA suggest that this regi

104 DHPS in plants is highly sensitive to end-product inhibition by lysine and, therefore, has an impo

105 The Ki values for R111M and R84M product inhibition by mevalonate 5-diphosphate are infla

106 The mechanism and magnitude of product inhibition by monophosphorylated EtsDelta138 is

107 ad-end inhibition by AMP and lumichrome, and product inhibition by NAD(+) indicated an ordered sequen

108 observed in the double-reciprocal plots for product inhibition by NADH and the dead-end inhibition b

109 Product inhibition by NADP is competitive vs NADPH and n

110 hat is zero order in superoxide and due to a product inhibition by peroxide anion.

111 ion, the mutant Y34F was more susceptible to product inhibition by peroxide than the wild-type enzyme

112 6-phosphate, and allosterically relieved of product inhibition by phosphate.

113 terminal half is necessary for the relief of product inhibition by phosphate.

114 Kinetic analysis of product inhibition by recombinant SPN demonstrated an or

115 Product inhibition by S-adenosylhomocysteine was competi

116 Product inhibition by saccharopine is uncompetitive vers

117 relief of the methylation reaction from end product inhibition by SAH and the subsequent increase in

118 oned DNA into neutral genomic sites prevents product inhibition by self-replicating plasmids, and has

119 of polyglutamate derivatives formed, and end-product inhibition by the major reduced folylpentaglutam

120 ein scission, and (ii) to prevent subsequent product inhibition by the NS3 C terminus.

121 r ADP, facilitating the efficient removal of product inhibition by this nucleotide.

122 Product inhibition by this P(i) pool silences the polyph

123 This phenomenon arises from competitive product inhibition by thrombin, which binds to prothromb

124 ociation of IDE with cellular regulators and product inhibition by Ub1-72 can prevent inadvertent pro

125 This product inhibition can attenuate ENPP1 during periods of

126 In an example of end-product inhibition, cholesterol accelerates the proteaso

127 The rate constant for formation of the product inhibition complex also decreases but to a much

128 ty is high and is held back only by a strong product-inhibition component to the catalytic process.

129 When an acetate product inhibition constant of 600+/-31M(-1), determined

130 nd apparent values for Km that depend on the product inhibition constant.

131 sented showing how the variation in apparent product inhibition constants (Kii) can be used to predic

132 Analysis of the product inhibition curve for 10-formyltetrahydrofolate d

133 nt with a Theorell-Chance BiBi mechanism and product inhibition data supported sequential binding of

134 Two-substrate, dead-end and product inhibition data, using analogues of ATP, are con

135 Initial velocity, product inhibition, dead-end inhibition, and equilibrium

136 orylation of the FASP serine cluster reduces product inhibition, decreasing PER2 stability and shorte

137 proline residues in a manner that diminishes product inhibition during collagen biosynthesis.

138 In addition, a product inhibition effect by ADP-ribose (through the reo

139 hich single mutations had little effect, was product inhibition eliminated in HKI.

140 hibited by peroxide, Q143N MnSOD exhibits no product inhibition even at concentrations of O2.

141 TP lid, which otherwise traps ADP, to remove product inhibition exerted by this nucleotide.

142 pectroscopy, bisubstrate kinetic assays, and product inhibition experiments to demonstrate that hNaa5

143 ta, along with equilibrium sedimentation and product inhibition experiments, suggest that steps invol

144 as investigated in initial rate kinetics and product inhibition experiments.

145 t in a mutant variant of Tp47 that overcomes product inhibition for the beta-lactamase activity, a no

146 how clear evidence for previously unreported product inhibition for the LuxAB reaction.

147 omponent of cellulase action on cellulose is product inhibition from monosaccharide and disaccharides

148 Product inhibition generates non-linearity in steady-sta

149 that of the wild-type enzyme and a level of product inhibition greater by approximately 2-fold.

150 trate (Pro) by only a single oxygen atom, no product inhibition has been observed for P4H.

151 on for analysis of kinetic time courses with product inhibition have been put forth.

152 onserved, active-site residue Tyr34 mediates product inhibition; however, the protein environment of

153 hand, the corresponding mutations eliminate product inhibition in a truncated form of HKI, consistin

154 ed inhibition of the protease, demonstrating product inhibition in addition to and distinct from subs

155 pincer catalysts that suffer from persistent product inhibition in ester hydrogenation.

156 To investigate the mechanism of product inhibition in MnSOD, two yeast MnSODs, one from

157 ing the reaction product ADP, which explains product inhibition in mPKs.

158 perfamily of enzymes, and forms the basis of product inhibition in Mug.

159 this advance, several issues and details of product inhibition in the intramolecular Schmidt reactio

160 ecDHFR, an evolutionary adaptation to reduce product inhibition in the NADP+ rich environment of prok

161 determined that kcat/K(m) was decreased and product inhibition increased for H30V MnSOD, both by 1-2

162 An analysis of the patterns of product inhibition indicated that RhlI catalyzes signal

163 2,6-bisphosphatase activity was sensitive to product inhibition; inhibition by inorganic phosphate wa

164 S3 (Solyc08g014230) encodes a functional end product inhibition-insensitive version of the committing

165 No product inhibition is observed, and competitive binding

166 by NMR methods, but RPKA methods reveal that product inhibition is operative, where tertiary amides a

167 Product inhibition is overcome through ligand-promoted p

168 ralization of a common regulatory motif (end-product inhibition) is sufficient both for learning cont

169 rnover-limiting Michael addition step, (iii) product inhibition, (iv) the catalyst resting state and

170 Initial velocity and product inhibition kinetic data are consistent with an o

171 Steady-state initial velocity and product inhibition kinetic studies indicate an ordered B

172 Product inhibition led to deactivated Pd but released li

173 , but suggest that other mechanisms, such as product inhibition, may also play a role in silencing in

174 Product inhibition negatively impacts cellulase action,

175 rt that the ARE genes are not subject to end product inhibition; neither ARE1 nor ARE2 transcription

176 can be directly applied to the phenomenon of product inhibition observed in natural Diels-Alderase en

177 Product inhibition occurs by an associative displacement

178 We identified product inhibition of accumulating acetic acid as a key

179 The patterns of product inhibition of AKT1, AKT2, and AKT3 by ADP were a

180 d to proteins is large enough to account for product inhibition of both pyridoxal kinase and pyridoxi

181 Finally, consistent with known product inhibition of CE-rich HDL by CE, CG simulations

182 active site, demonstrating a common mode of product inhibition of CK1delta.

183 consolidating process steps and reducing end-product inhibition of enzymes compared with separate hyd

184 also occurred in these experiments, although product inhibition of GAD(67) by increased GABA could pl

185 hesis is regulated, at least in part, by end-product inhibition of glutamine PRPP amidotransferase.

186 versus CHB-glutathione conjugate: formation, product inhibition of GSTA1-1 catalysis, and transport b

187 For MLP detoxification, where product inhibition of GSTA1-1 is less important, GSTA1-1

188 hypotheses have been proposed to account for product inhibition of HKI.

189 , although larger amounts led to competitive product inhibition of hydrolytic activity.

190 enosylhomocysteine (AdoHcy), which causes by-product inhibition of methyltransferases (MTase's).

191 broblasts from affected individuals, and end-product inhibition of PSS1 by phosphatidylserine was mar

192 When neglecting acetate product inhibition of the acylase, values for k(cat) wer

193 ditions, ADP competes with ATP, resulting in product inhibition of the ATPase rate.

194 resistance to CHB in order to relieve potent product inhibition of the enzyme by intracellular CHB-SG

195 ingle mutants could be due to alleviation of product inhibition of the enzyme.

196 te of cellular methyltransferases results in product inhibition of the enzyme.

197 The high stereoselectivity and minimal product inhibition of the evolved enzyme enabled prepara

198 Patterns of product inhibition of the forward reaction were consiste

199 conversion of cystine to cysteine precludes product inhibition of the importer, so cystine import co

200 Evidence was also obtained for product inhibition of the protease by the cleaved C term

201 kinetic mechanisms in terms of substrate and product inhibition of the recombinant human (rh) protein

202 or dCTP (not deoxyguanosine or dGTP) and end-product inhibition of the respective activities by dATP

203 isoforms in a 5-fold excess of 18:1-ACP show product inhibition of up to 73%.

204 This correlates with the enhanced product inhibition of Y34F during the catalysis of O-(2)

205 a suggest that elevated levels of pGpG exert product inhibition on EAL-dependent PDEs, thereby increa

206 he rate law and demonstrate the influence of product inhibition on the kinetics of the intramolecular

207 In vitro analysis showed strong product inhibition on the T507A mutant with particular s

208 tro tests of possible activity regulation by product inhibition or by Akt1 binding gave negative resu

209 o-order kinetic behavior reflect competitive product inhibition or self-inhibition by substrate.

210 Kinetic and product inhibition parameters are derived by converting

211 We recently determined a product-inhibition pathway for the diguanylate cyclase r

212 re qualitatively similar with respect to the product inhibition patterns and the pH dependence of kin

213 The steady-state initial velocity and product inhibition patterns are consistent with an order

214 was also followed when 3-PGA was absent, but product inhibition patterns changed dramatically.

215 In this work, product inhibition patterns for the synthase activity of

216 rst, two-substrate steady-state kinetics and product inhibition patterns indicated a Steady-State Ord

217 The substrate specificities and product inhibition patterns of haloalkane dehalogenases

218 ctive conformation of the enzyme and resists product inhibition, potentially allowing for the highly

219 cid substrates with low efficiency, and that product inhibition primarily affects preferred substrate

220 onsideration of the expanded set of reaction products, inhibition rate constants were measured for a

221 attenuation of TET-2 activity is likely via product inhibition rather than competitive inhibition.

222 Of note, we also found that glucose product inhibition regulates the activities of both SI s

223 ted in a mutant of human MnSOD with weakened product inhibition resembling that of E. coli MnSOD.

224 PNPOx has been proposed to undergo product inhibition resulting from PLP binding at the act

225 ue k(2)/k(3) that characterizes the level of product inhibition scales as ScMnSOD > D. radiodurans Mn

226 ent paper describes the use of steady-state, product inhibition, single-turnover, and kinetic simulat

227 Overall, these results depict a novel product inhibition strategy in which shared substrate an

228 xide dismutase (MnSOD) is characterized by a product inhibition stronger than that observed in bacter

229 Product inhibition studies also support a sequential ord

230 The results of product inhibition studies and isotope effects suggest t

231 rates and products bind the free enzyme, and product inhibition studies are consistent with a random

232 Product inhibition studies demonstrate that CoA binds co

233 pyrophosphorolysis of IMP, GMP, and XMP and product inhibition studies have been used to elucidate t

234 Product inhibition studies have led to a proposed uni-bi

235 Substrate binding and product inhibition studies helped to further elucidate t

236 Product inhibition studies indicate that ADP is competit

237 e for fructose 1, 6-bisphosphate 16-fold and product inhibition studies indicate that this effect is

238 Analysis of product inhibition studies indicated that dCyd kinase fo

239 locity plots with other phosphate donors and product inhibition studies indicated that dCyd kinase fo

240 Initial velocity and product inhibition studies indicated that LdAPRT follows

241 Product inhibition studies indicated that the forward re

242 The minimum kinetic model from the data in product inhibition studies is an ordered bi-bi mechanism

243 Product inhibition studies resulted in two competitive a

244 Steady-state kinetic and product inhibition studies revealed that PP2Calpha emplo

245 Product inhibition studies revealed that the product CoA

246 Steady-state kinetic and product inhibition studies showed that SMYD2 operates vi

247 Product inhibition studies suggest that ADP is the last

248 sed and purified GmHSD, initial velocity and product inhibition studies support an ordered bi bi kine

249 However, product inhibition studies were only consistent with the

250 thionine (AdoMet) as variable substrates and product inhibition studies with methylated DNA and S-ade

251 Product inhibition studies with PP(i) and DGPC demonstra

252 ide and S-adenosyl-L-methionine (AdoMet) and product inhibition studies with S-adenosyl-L-homocystein

253 Product inhibition studies with SUV39H1 showed that S-ad

254 combination of bisubstrate kinetic analysis, product inhibition studies, and dead-end competitive inh

255 acterization, utilizing initial velocity and product inhibition studies, found the mechanism of PPCS

256 In product inhibition studies, thymidine inhibited noncompe

257 On the basis of product inhibition studies, we propose that the reaction

258 estigated using initial velocity methods and product inhibition studies.

259 lt contradicts previous conclusions based on product inhibition studies.

260 velocity analysis and substrate analogue and product inhibition studies.

261 Pong Bi Bi mechanism, which was verified by product inhibition studies.

262 Investigation of the kinetic mechanism using product inhibition suggested that a compulsory-ordered t

263 Theoretical analysis of product inhibition suggested that the inhibition strengt

264 The newly detected concerted substrate and product inhibition suggests that TryS activity is tightl

265 SOD exhibits a substantially higher level of product inhibition than the MnSODs from bacteria.

266 tion of a reaction network with an important product inhibition that is in agreement with the experim

267 in which a DNA template catalyses, with weak product inhibition, the production of sequence-specific

268 The use of dead-end and product inhibition, the solvent isotope effect, and the

269 Typically, product inhibition-the tendency of products to bind to t

270 sing enzyme concentration, strongly supports product inhibition through hexamer stabilization.

271 Nevertheless, AlkA exhibits significant product inhibition under multiple-turnover conditions, a

272 SPN was unaffected by product inhibition using nicotinamide, suggesting that t

273 mmalian GTP cyclohydrolase is subject to end-product inhibition via an associated regulatory protein

274 HyH product inhibition was observed with emodin analogues, r

275 Moreover, the mechanism of this weakened product inhibition was similar to that in E. coli MnSOD,

276 vide molecular level insights into cellulase product inhibition, we examine the impact of product bin

277 nt enzyme was 2.7-fold more sensitive to CTP product inhibition when compared with the phosphorylated

278 loading has often been necessary to overcome product inhibition, where the product amide irreversibly

279 Manganese- and iron-containing SOD exhibit product inhibition whereas Cu/ZnSOD does not.

280 MPG plays a critical role in overcoming the product inhibition, which is achieved by reducing the di

281 ew reagent, X10-23, overcomes the problem of product inhibition, which limited previous 10-23 designs

282 ng constants for cellulases and suggest that product inhibition will vary significantly based on the

283 However, this mutant exhibited a strong product inhibition with a zero-order region of superoxid

284 d CTP synthetases were less sensitive to CTP product inhibition with inhibitor constants for CTP of 8