ライフサイエンスコーパス: protamine

1 gulation: citrate and calcium or heparin and protamine.

2 sible, selective, and sensitive detection of protamine.

3 aboratory from enzymatic digestion of native protamine.

4 calcium or the polycationic heparin antidote protamine.

5 , and activated platelets in the presence of protamine.

6 quickly reverse the effects of heparin using protamine.

7 nd Escherichia coli to become susceptible to protamine.

8 ticoagulant polyion heparin and its antidote protamine.

9 polyion sensors for the detection of polyion protamine.

10 er separation from CPB and administration of protamine.

11 ished promptly after heparin antagonism with protamine.

12 ible electrochemical sensors for the polyion protamine.

13 he determination of the polyions heparin and protamine.

14 ch is close to the excess positive charge of protamine.

15 t activity without the lung injury seen with protamine.

16 e strong binding reaction of polyanions with protamine.

17 selective and sensitive for the detection of protamine.

18 nation of trace amount of an important drug, protamine.

19 ntial based on cation-exchange extraction of protamine.

20 omatin compacted primarily by sperm-specific protamines.

21 d by transition proteins and subsequently by protamines.

22 fore eventual replacement of histones by the protamines.

23 is achieved by replacement of histones with protamines.

24 lation and replacement of most histones with protamines.

25 n of transition nuclear protein 1 (Tnp1) and protamine 1 (Prm1) genes, the products of which are requ

26 nes Tnp1 (transition protein 1), Tnp2, Prm1 (protamine 1), and Prm2, all of which are essential for c

27 ethylation and the exchange of histones with protamines(1,2).

28 Our data demonstrate that, like protamine-1 and -2, both alleles of Klhl10 are required

29 expression of Transition protein 2 (TP2) and Protamine 2 (Prm2) proteins (chromatin remodelers, essen

30 , NLP, and Nph share roles in the removal of protamine A.

31 Ex vivo and in vivo, protamine abolishes well-known apelin effects, such as a

32 id decrease in platelet count directly after protamine administration, we determined the incidence an

33 doped polymeric membranes but also to reveal protamine adsorption at the membrane/water interface.

34 Protamine adsorption is thermodynamically more favorable

35 only recently recognized immune response to protamine after cardiopulmonary bypass (CPB) surgery wit

36 Compared to protamine, all the LMWP peptides showed lower DNA bindin

37 tamine and heparin were present but not with protamine alone.

38 od and Drug Administration-approved compound protamine-already used clinically after cardiac surgery-

39 ivity, which confers bacterial resistance to protamine and alpha-helical CAMPs.

40 A and amiC, elevated bacterial resistance to protamine and alpha-helical peptides magainin 2 and meli

41 orption reactions of the natural polypeptide protamine and also for Ca(2+) and Mg(2+) transfers facil

42 ption on microchannel surfaces modified with protamine and biotin, respectively, whereas bovine serum

43 ically relevant polyions such as heparin and protamine and drastically improve the sensitivity of ion

44 e real-time detection of injected samples of protamine and heparin at up to 20 samples/h.

45 We hypothesized that patients exposed to protamine and heparin during CPB will develop antibodies

46 ntibodies induced thrombocytopenia only when protamine and heparin were present but not with protamin

47 on mixed potential responses, for which both protamine and Na(+) need to be transferred simultaneousl

48 in and alpha-chymotrypsin are detected using protamine and synthetic polycationic oligopeptides that

49 GLD2 also affects the incorporation of protamines and the stability of dynamin and transition p

50 duction of intermolecular disulfide bonds in protamines and their eviction from sperm during fertiliz

51 y complete elimination of those encoding the protamines and transition proteins, but was not associat

52 ration-free methodology for the detection of protamine (and, by titration, heparin) in undiluted huma

53 A divalent cation (calcium), a polyion (protamine), and an anion (chloride) were successfully de

54 in nucleosomes and histone variants (and not protamine), and we find linker histones high and H4K16ac

55 (trypsin) based on proteolytic digestion of protamine, and polyanions (pentosan polysulfate and hepa

56 Remarkably, protamine antagonist activity is fully reversed by hepar

57 ross-reactivity toward the mice-derived anti-protamine antibodies.

58 We designed a protamine-antibody fusion protein to deliver siRNA to HI

59 events in the group assigned to heparin and protamine anticoagulation (11 vs 2; p = 0.011).

60 cuit life compared with regional heparin and protamine anticoagulation, does not affect cytokine leve

61 on cytokine levels compared with heparin and protamine anticoagulation.

62 ous polyamines and the heterogeneous protein protamine are quite similar, demonstrating the universal

63 Protamines are arginine-rich IDPs involved in the conden

64 to sperm chromatin remodeling, during which protamines are expelled and replaced by histones.

65 Protamines are small, highly positively charged peptides

66 Protamines are synthesized during spermiogenesis and con

67 om histone H2A has been developed to replace protamine as a conditionally reversible, nucleic acid co

68 itro and in vivo than the NPs assembled with protamine as the nucleic acid condensing agent.

69 Reversible adsorption of protamine at low concentrations down to 0.038 mug/mL is

70 Adsorptive preconcentration of protamine at the membrane/water interface is quantitativ

71 32 is required for the removal of Drosophila protamine B in vitro, whereas NAP-1, NLP, and Nph share

72 ly development to facilitate the switch from protamine-based sperm chromatin structures to the somati

73 ntly irreversible potentiometric response to protamine because back-extraction of protamine from the

74 Conversely, protamine binds to the fibrin clot, which could explain

75 demonstrate a new pharmacologic property of protamine-blockade of APJ-that could explain some advers

76 ased resistance to the antimicrobial peptide protamine both in ompT mutants and in wild-type E. coli

77 is exhibited hypersusceptibility not only to protamine but also to alpha-helical cathelicidin LL-37 a

78 colorimetric response to polycations such as protamine but not to small inorganic cations because onl

79 s reduce arthritis by 19%, our anti-C5aR1 Ab-protamine-C5 siRNA conjugate was effective in reducing a

80 We then generated a novel anti-C5aR1 Ab-protamine-C5 siRNA conjugate.

81 Protamine calibration curves were recorded at physiologi

82 can inhibit their oxidase-like activity and protamine can recognize heparin, we prepared the protami

83 Protamine chaperone TAP/p32 dissociates DNA-protamine co

84 Here we identify four Drosophila protamine chaperones that mediate the dissociation of pr

85 just before histone-to-transition protein-to-protamine chromatin remodelling in spermiogenesis.

86 hd5) as a master regulator of the histone-to-protamine chromatin remodelling process.

87 harboring a single copy insert of the human protamine cluster were subjected to Micrococcal Nuclease

88 usion protein (F105-P) was designed with the protamine coding sequence linked to the C terminus of th

89 Protamine chaperone TAP/p32 dissociates DNA-protamine complexes in vitro only when protamine oligome

90 These materials include covalent conjugates, protamine complexes, nanoparticles based on lipids or po

91 was continuously monitored by measuring the protamine concentration as it is cleaved by enzyme into

92 In this method with increasing the protamine concentration, the fluorescence of the quantum

93 xhibited a stable and reversible response to protamine concentrations ranging from 0.05 to 30 mg L-1.

94 eparin followed by complexation with albumin-protamine conjugate, termed 'camouflage'.

95 arin complexes, but were cross-reactive with protamine-containing insulin preparations.

96 l biosensor, under the optimized conditions, protamine could be measured in the range of 2.0-200 ng m

97 erved that the neutral amino acids in salmon protamine decrease the net attraction between protamine-

98 wer, while means of sperm DNA fragmentation, protamine deficiency, and lipid peroxidation were signif

99 e, an ErbB2 single-chain antibody fused with protamine delivered siRNAs specifically into ErbB2-expre

100 fluence of common interfering species on the protamine detection was studied.

101 With the diffusion coefficient of protamine determined to be (1.2 +/- 0.1) x 10(-6) cm(2)/

102 ely helps open up such structures to enhance protamine dismissal by nucleoplasmin (NPM2) and enable t

103 Protamine displays a 390-nM affinity for APJ and behaves

104 osphoramidate-dipropylamine) (PPA) and Lipid-Protamine-DNA (LPD) nanoparticles consistently showed th

105 derstand the nature of the forces condensing protamine-DNA assemblies and their dependence on amino a

106 chaperones that mediate the dissociation of protamine-DNA complexes: NAP-1, NLP, and nucleophosmin a

107 rotamine decrease the net attraction between protamine-DNA helices compared with the equivalent homo-

108 bservations have biological implications for protamine-DNA packaging in sperm heads.

109 A model is also presented for the bull protamine.DNA complex in native sperm cell chromatin tha

110 rgo to the cytoplasm than our previous lipid/protamine/DNA (LPD) formulation, leading to a significan

111 .005, 0.89 +/- 0.01, and 0.065 +/- 0.008 for protamine, DNNS, and the complex, respectively.

112 ine(n+) (aq) right harpoon over left harpoon protamine-DNNS complex (DCE).

113 the MARs bounding the PRM1 --> PRM2 --> TNP2 protamine domain have many and varied functions.

114 potentiation an approximately 13.7-kb mouse protamine domain of increased nuclease sensitivity flank

115 x attachment regions (MARs) encompassing the protamine domain were created.

116 the multigenic PRM1 --> PRM2 --> TNP2 human protamine domain.

117 reveal its role in regulating the histone-to-protamine exchange during spermiogenesis.

118 G RNA depletion results in defective histone-protamine exchange, sperm maturation and chromatin organ

119 ross chromatin remodeling that occurs during protamine exchange.

120 in-associated bleeding in surgical settings, protamine, exhibits adverse effects.

121 ion is thermodynamically more favorable than protamine extraction as shown by cyclic voltammetry at p

122 ion-transfer voltammetry not only to confirm protamine extraction into ionophore-doped polymeric memb

123 The voltammetrically reversible protamine extraction results in an apparently irreversib

124 otentiometric protamine response is based on protamine extraction.

125 d to study the phase transfer of polypeptide protamine facilitated by complexation with charged ionop

126 fide bonds hold the terminal domains of bull protamine folded back onto the central DNA binding domai

127 Based on available evidence, the use of protamine following CEA is associated with a reduction i

128 undergoes a massive exchange of histone with protamine for compaction into sperm during spermiogenesi

129 leic acid therapeutics, which is captured by protamine for siRNA delivery.

130 low immunogenicity, they would be safer than protamine for use in gene therapies.

131 After fertilisation, sperm DNA exchanges protamines for histones recruited from oocyte cytoplasm,

132 of sperm chromatin remodeling that exchanges protamines for histones to form the nucleosome-based chr

133 fertilization, the paternal genome exchanges protamines for histones, undergoes DNA demethylation, an

134 nzymatically to produce low molecular weight protamine fragments (LMWPs) of various lengths and amino

135 onse to protamine because back-extraction of protamine from the membrane extremely slows down at the

136 Cation-exchange extraction of polypeptide protamine from water into an ionophore-based polymeric m

137 Protamines from eutherian mammals, including bulls and h

138 ondary structure is also critical for proper protamine function.

139 We show here that antibody-protamine fusion proteins targeting the human integrin l

140 s and reveals transition nuclear protein and protamine genes as direct targets of JHDM2A.

141 rs (95% CI, 13.3-34.0 hr) in the heparin and protamine group (log rank p = 0.0037, 204 circuits).

142 e serum (group 2), StemPro medium (group 3), protamine (group 4), and protamine plus heparin (group 5

143 h long-acting (glargine, detemir) or neutral protamine Hagedorn (NPH) insulin between 2002 and 2012,

144 nsulin product than the conventional neutral protamine Hagedorn (NPH) insulin for diabetic control.

145 strong rationale favoring glargine, neutral protamine Hagedorn insulin, insulin detemir, or fixed-ra

146 ween insulin type (basal, pre-mix or Neutral Protamine Hagedorn, NPH) while ANCOVAs compared haemoglo

147 ion-selective electrodes for the polycation protamine have been reported, for instance, a pulsed chr

148 rmolecular disulfide bonds between DNA-bound protamines help stabilize the chromatin of mature mammal

149 surgery, 57 (9.6%) tested positive for anti-protamine-heparin antibodies at baseline and 154 (26.6%)

150 Seven patients had platelet-activating, anti-protamine-heparin antibodies at baseline and showed a gr

151 Platelet-activating anti-protamine-heparin antibodies show several similarities w

152 as a risk factor for the development of anti-protamine-heparin antibodies.

153 ical biomolecules to build up a thin film of protamine-heparin complex on Kapton, but also the first

154 during CPB will develop antibodies (Abs) to protamine/heparin (PRT/H) complexes that are capable of

155 that CidB targets nuclear-protein import and protamine-histone exchange and that CidA rescues embryos

156 , also identified karyopherin-alpha; the P32 protamine-histone exchange factor bound as well.

157 of catalase layers is compared with that of protamine, horseradish peroxidase, and inactivated catal

158 We observed that the ferumoxytol-heparin-protamine (HPF) nanocomplexes were stable in serum-free

159 The primary structure of mammalian protamine I can be divided into three domains, a central

160 Optical determination of protamine in human blood plasma using the exhaustive nan

161 Because nucleosomes are widely replaced by protamine in mature human sperm, the epigenetic contribu

162 s successfully used for the determination of protamine in real samples.

163 monstrated to be useful for the detection of protamine in the therapeutic range of undiluted human bl

164 ient selectivity for the direct detection of protamine in undiluted whole blood samples.

165 erized conformational ensembles for a set of protamines in aqueous milieus using molecular simulation

166 es (K(+) , Na(+) , and Cl(-) ) and polyions (protamine) in sub-nanoliter aqueous droplets.

167 DA)-approved drugs--ferumoxytol, heparin and protamine--in serum-free medium to form self-assembling

168 rm cell chromatin reorganization and reduced protamine incorporation.

169 ckdown podocytes were also protected against protamine-induced injury.

170 te the prodrug feature, and subsequently the protamine-induced reversal of heparin inhibition to resu

171 to the fibrin clot, which could explain how protamine instigates clot lysis and increases bleeding a

172 The stoichiometry for protamine interaction with ODSH is determined to average

173 Protamine is a natural DNA condensation agent and has be

174 a potentiometric super-Nernstian response to protamine is also observed.

175 Protamine is an antagonist of apelin receptor, and its a

176 er analysis of these structures suggest that protamine is bending the DNA to achieve this curvature r

177 The polyion protamine is detected in 10-fold diluted blood samples i

178 The amino acid content alone of salmon protamine is enough to rationalize the forces that packa

179 Protamine is routinely used to reverse heparin anticoagu

180 This suggests that protamine is using a multi-step process to loop the DNA

181 A striking and general feature of protamines is the almost exclusive use of arginine over

182 ies compared to salmon nuclei despite salmon protamine lacking cysteine residues.

183 Cysteine oxidation in protamines leads to their oligomerization and contribute

184 improvement of the sensor sensitivity to low protamine levels.

185 Low molecular weight protamine (LMWP) is a peptide fragment produced in our l

186 e naturally derived CPP low-molecular-weight protamine (LMWP) to modify PLGA NP for enhanced drug del

187 after conjugation with low molecular weight protamine (LMWP), a cell penetrating peptide (CPP), insu

188 mbination methods, with low molecular weight protamine (LMWP), a cell-penetrating peptide (CPP) which

189 P) developed in our lab-low molecular weight protamine (LMWP).

190 igonucleotide (ASO) and low-molecular weight protamine (LMWP).

191 We therefore conclude that protamine loops DNA in multiple steps, bending it into a

192 Short arginine-rich proteins called protamines mediate the near crystalline DNA packaging in

193 th ODSH is determined to average 1.39 microg protamine/microg ODSH in plasma.

194 amine can recognize heparin, we prepared the protamine-modified Pt NPs through direct adsorption on t

195 m(2)/s, the ionic charge transferred by each protamine molecule was obtained as +20 +/- 1, which is c

196 voltammograms are based on desorption of all protamine molecules that are transferred across the inte

197 olecular disulfide bonds formed between bull protamine molecules within in vitro DNA condensates is c

198 ane (DCE) interfaces, i.e., sDNNS(-) (DCE) + protamine(n+) (aq) right harpoon over left harpoon prota

199 Both charge number of transferred protamine, n, and complexation stoichiometry, s, were de

200 o that obtained by using Ferumoxytol-heparin-protamine nanocomplex; and (ii) cells can be re-sized to

201 Although the cross-linked protamine network is known to stabilize the resulting nu

202 s DNA-protamine complexes in vitro only when protamine oligomers are first converted to monomers by D

203 s the direct injection and online removal of protamine-oligonucleotide nanoparticles ("proticles") wi

204 rface probes the clinical heparin antagonist protamine or the physiological partner antithrombin III

205 ns are further substituted by sperm-specific protamines, P1 and P2, to form a highly condensed sperm

206 es is largely erased in early pronuclei in a protamine phosphorylation-dependent manner, suggesting t

207 o medium (group 3), protamine (group 4), and protamine plus heparin (group 5).

208 anscription of Transition proteins (Tnp) and Protamines (Prm), exhibiting chromatin compaction defect

209 on of transition protein (Tnp1 and Tnp2) and protamine (Prm1 and Prm2) genes, which are critical for

210 ssion of transition proteins (Tnp1/Tnp2) and protamines (Prm1/2).

211 Protamine proteins dramatically condense DNA in sperm to

212 ted 2 unrelated positively charged proteins, protamine (PRT) and lysozyme, and studied H-dependent in

213 ined the incidence and clinical relevance of protamine-reactive antibodies in patients undergoing car

214 In summary, we identified factors mediating protamine removal from DNA and reconstituted in a define

215 e nascent male pronucleus, the machinery for protamine removal remains largely unknown.

216 d5 as a multi-faceted mediator of histone-to-protamine replacement and depict the cascade of molecula

217 ed in genomic regions that escape histone to protamine replacement in human and mouse sperm.

218 on to adsorption, voltammetric extraction of protamine requires approximately 0.2 V more negative pot

219 ation of the CpxR/CpxA system can facilitate protamine resistance because nlpE overexpression elevate

220 s agreement confirms that the potentiometric protamine response is based on protamine extraction.

221 e intermolecular sulfur-sulfur bonds of bull protamine results in tighter DNA packing.

222 Systemic delivery of lipid-protamine-RNA nanoparticles encapsulating a chemically m

223 sample (5.8 muL) confined between a tubular protamine selective membrane (inner diameter, 600 mum) a

224 containing dinonylnaphthalenesulfonate as a protamine-selective ionophore.

225 The protamine-selective solid-contact sensor was fabricated

226 trypsin digestion are not detectable by the protamine-sensing membrane.

227 The protamine-sensing optode platform is used to indirectly

228 A protamine-sensitive mutant identified carried the transp

229 SH in buffer and plasma is described using a protamine-sensitive polymer membrane electrode as the de

230 Here, such protamine-sensitive pulstrodes are applied for the real-

231 The protamine sensors exhibited a stable and reversible resp

232 electrostatic attraction between heparin and protamine-stabilized Pt NPs induced nanoparticle aggrega

233 insight into its potential to be a clinical protamine substitute as well as a non-toxic cell penetra

234 In a typical case, coassembly of protamine sulfate and perylene dye via electrostatic att

235 The effects of protamine sulfate and vanadate on Nephrin phosphorylatio

236 sulated with pancreatic islet cells by using protamine sulfate as a clinical-grade alginate cross lin

237 Protamine sulfate can be administered at the conclusion

238 s and other mammalian cells with ferumoxides-protamine sulfate complexes (FE-Pro), cellular toxicity,

239 with wild-type littermates injured by either protamine sulfate or nephrotoxic serum (NTS).

240 n subjected to podocyte injury in vivo using protamine sulfate or nephrotoxic serum (NTS).

241 In a mouse model of podocyte injury, protamine sulfate perfusion of the Cfl1 mutant mouse ind

242 e podocyte foot process effacement following protamine sulfate perfusion.

243 Administration of protamine sulfate rapidly damaged the isolated glomeruli

244 ons in this model using high-dose heparin or protamine sulfate support the pathogenic role of surface

245 AC133(+) cells labeled with ferumoxide-protamine sulfate were mixed with either rat glioma or h

246 ated SPIO used as an MRI contrast agent, and protamine sulfate, conventionally used to reverse hepari

247 l facet cell QIRs with the cationic protein, protamine sulfate, led to epithelial exfoliation and era

248 Cationic protamine sulfate, which forms similar complexes with he

249 and fluorescent dye permeability of control, protamine sulfate- and lipopolysaccharide-treated denude

250 d chelation of extracellular calcium reduced protamine sulfate-induced damage, suggesting that calciu

251 eficient mice display impaired recovery from protamine sulfate-induced foot process effacement and li

252 On the contrary, protamine sulfate-induced phosphorylation at Tyr-1176/11

253 npo(-/-) mice display impaired recovery from protamine sulfate-induced podocyte foot process (FP) eff

254 the cathepsin L inhibitor E64 all inhibited protamine sulfate-mediated barrier changes, which sugges

255 e coagulation assays, and reversibility with protamine sulfate.

256 f B. anthracis and screened them for altered protamine susceptibility.

257 remixed biaspart insulin (70% insulin aspart protamine suspension and 30% insulin aspart of rDNA orig

258 e positions of the cysteine residues in bull protamine that form intermolecular disulfide bonds.

259 , ascorbic acid 6-palmitate and salmon sperm protamine, that effectively inhibited anthrax cytotoxic

260 In the presence of a given level of protamine the initial rate of reaction can be linearly r

261 catalyzing site-specific phosphorylation of protamine, thereby triggering protamine-to-histone excha

262 rin concentration in whole blood samples via protamine titration are demonstrated.

263 lity of the technique for monitoring heparin/protamine titrations in physiological saline solutions i

264 Heparin-protamine titrations were performed in undiluted human b

265 oposed model for binding of polyarginine and protamine to DNA provides a convenient framework for und

266 ellulose acetate filter membrane coated with protamine to eliminate addition of the indicator polycat

267 Heparin interacts with protamine to form ultralarge complexes that are immunoge

268 The addition of protamine to the medium or treatment of the cells with c

269 then reacted with either HIV-Tat peptide or protamine to yield a nanoparticle with membrane-transloc

270 matin histones are replaced by arginine-rich protamines to densely compact DNA in sperm heads.

271 on of the replacement of somatic histones by protamines to epigenetic control of gene transcription.

272 ch histones are replaced with sperm-specific protamines to repackage the genome into the highly compa

273 asaccharide Arixtra and polycationic peptide protamine, to yield the membrane permeability that is lo

274 phorylation of protamine, thereby triggering protamine-to-histone exchange in the fertilized oocyte.

275 This approach linked robust endogenous protamine transcription and transgene suppression to its

276 to quantitatively and mechanistically assess protamine transfer at ionophore-based polymeric membrane

277 an irreversible transient CV of facilitated protamine transfer gives an apparent k(0) value of 3.5 x

278 ned current responses based on the selective protamine transfer were obtained reproducibly even in th

279 n unclear, appears to disrupt the histone-to-protamine transition in drive-sensitive spermatids beari

280 rate corresponding defects in the histone to protamine transition.

281 ssion of select post-meiotic genes including protamines, transition protein 2, and H1fnt, all of whic

282 uld explain some adverse effects observed in protamine-treated patients.

283 ositive cells were observed in comparison to protamine treatment.

284 Interestingly, protamine undergoes a DNA-dependent phase transition to

285 Protamine use remains controversial owing to concern for

286 significantly between patients who received protamine vs those who did not for the following outcome

287 replicates determination of 30.0 ng mL(-)(1) protamine was 1.26%.

288 The use of protamine was associated with a significant decrease in

289 In this work, protamine was selectively digested enzymatically to prod

290 The polyion protamine was used as a substrate to detect the activity

291 A DNA payload, pre-compressed by protamine, was encapsulated into the liposomes, which di

292 ure the dynamic, real-time looping of DNA by protamine, we observe the presence of multiple folded st

293 Sharply contrasting protamine, we show that UHRA does not interact with fibr

294 ent therapy anticoagulation with heparin and protamine were more likely to experience circuit clottin

295 Protamine, which is routinely used after cardiac surgery

296 calcium, chloride, alkalinity, acidity, and protamine with a range of ion-selective membranes.

297 perties, interresidue contact preferences of protamines with similar polymeric attributes suggest tha

298 biocompatibility profile similar to that of protamine, with minimal immunostimulating and systemic t

299 t the established antiangiogenic activity of protamine would rely on APJ antagonism.

300 trations of ODSH with the heparin antagonist protamine yield sharp endpoints with sensitivity to ODSH