ライフサイエンスコーパス: protease activity

1 inX1 at the site of Q50-G51 pair through its protease activity.

2 s, but TRAP1 is not a direct target of HTRA2 protease activity.

3 old improvement in sensitivity for detecting protease activity.

4 e S. aureus exposure blocked the increase in protease activity.

5 -mediated antiviral action is independent of protease activity.

6 ific protease gene expression and intestinal protease activity.

7 vel allosteric modulators of gamma-secretase protease activity.

8 nsor which monitors the single cancer cells' protease activity.

9 erate active site, rendering it incapable of protease activity.

10 SFV to early endosomes or change their pH or protease activity.

11 s recruited to the MIB-IgG complex, enabling protease activity.

12 vation of plant defence independently of its protease activity.

13 None of the mutations affected NS2B-NS3 protease activity.

14 cted humans secrete wild-type levels of SpeB protease activity.

15 t major USP18 functions are unrelated to its protease activity.

16 A total of 88% of 169 strains express IgA protease activity.

17 tion to bystander food antigen through their protease activity.

18 may explain how ClpT1,2 contribute to ClpPR protease activity.

19 ts chromophore incorporation is regulated by protease activity.

20 -acid modification within Pla that optimizes protease activity.

21 endent on T. foetus cell-associated cysteine protease activity.

22 of TAB2 and its partners, which requires the protease activity.

23 This cleavage relies on 3C(pro)'s cysteine protease activity.

24 d was not suppressed by inhibiting trypsin's protease activity.

25 otein often are not good indicators of total protease activity.

26 nfluences motility and virulence, as well as protease activity.

27 se of activity-based probes (ABPs) to detect protease activity.

28 s epithelial integrity on exposure to pollen protease activity.

29 neri type III effector protein with cysteine protease activity.

30 d levels of caspase 9, followed by caspase 3 protease activity.

31 ns that inhibited HCV replication and NS3/4A protease activity.

32 f microtubule regrowth also required 3C(pro) protease activity.

33 not require a significant increase in ADAM17 protease activity.

34 to inhibition of lysosomal cathepsin b-like protease activity.

35 viral life cycle cannot be completed without protease activity.

36 onset of fluorescence correlated with viral protease activity.

37 e used as reporter substrates of unregulated protease activity.

38 6 hours by targeting the bacteria's surface protease activity.

39 P2 that has reduced DUB activity but retains protease activity.

40 d may allow for a pH-dependent regulation of protease activity.

41 e a cellular response to pathogen-associated protease activity.

42 n to bind neutrophil elastase and to inhibit protease activity.

43 d by Akt on Ser173 and Ser181, enhancing its protease activity.

44 d non-inflammatory CHS with increased serine protease activity.

45 stabilizing Bet v 1 and inhibiting cathepsin protease activity.

46 uces its cleavage, which is dependent on the protease activity.

47 rasuis (HpHtrA) exhibited both chaperone and protease activities.

48 at can be utilized for probing extracellular protease activities.

49 thway independent of the encoded papain-like protease activities.

50 ssing independent of the encoded papain-like protease activities.

51 ts to be associated with certain serine endo-protease activities.

52 tforms provide limits of quantitation to ~1% protease activity (~60 pM enzyme concentration) in <1 h

53 l studies define a DNA switch triggering its protease activity, a ubiquitin switch controlling SPRTN

54 multiple peptide-based evidence on intrinsic protease activity affecting several HAdV proteins.

55 lso seen in wild-type cells lacking vacuolar protease activity after induction of a DSB.

56 to ClpP is therefore not required for ClpAP protease activity, although some flexibility in how the

57 By altering host protease activities and the degradation patterns of prot

58 2) chimeric Sindbis virus system to evaluate protease activities and the efficacy of inhibitors direc

59 fe and efficient platforms to evaluate viral protease activities and the efficacy of protease inhibit

60 from distal unburned skin exhibited greater protease activity and a reduced capacity to inhibit bact

61 ATP, revealing how ATP binding regulates the protease activity and access to the translocation pathwa

62 wth in culture, an effect independent of its protease activity and achieved through epidermal growth

63 ct EV subsets that differ in composition and protease activity and are indicative of differential imm

64 strated calcium-dependent and AprI-inhibited protease activity and cytotoxicity to airway and ocular

65 rtS and slpB genes was defective in secreted protease activity and cytotoxicity to human cell lines.

66 A positive correlation between protease activity and cytotoxicity to human corneal epit

67 double-mutant 3CLpro enzyme as impaired for protease activity and exhibiting reduced sensitivity to

68 sses the viral polyprotein with its cysteine protease activity and helps EV71 replicate through a che

69 ream of DA1, DAR1 and DAR2 to restrict their protease activity and hence fine-tune plant growth and d

70 CG1 and 3 of 3 CARD11 variants induced MALT1 protease activity and increased transcription from NFAT

71 Canonical Wnt signaling increased protease activity and induced cartilage damage shortly a

72 ivity assays, the mutation increased calpain protease activity and made it far more active at low con

73 lammatory modulator in humans that regulates protease activity and NET formation and modifies efferoc

74 regulatory signaling that modulates secreted protease activity and promotes cell wall function at hig

75 alkalinization reduces cytosolic cathepsin L protease activity and protects the podocyte cytoskeleton

76 aluable a tool for the real-time analysis of protease activity and regulation.

77 instrumental in the detection and control of protease activity and serve as alternative methods to ge

78 The high protease activity and stability established plus the low

79 p confirmed the RNAIII is required to induce protease activity and that agr cross talk modulates Ecp

80 ped here allow for rapid evaluation of viral protease activity and the identification of protease inh

81 the autolysis loop in mesotrypsin decreases protease activity and thereby protects the pancreas agai

82 c [Ca(2+)]i elevation, necrosis, and trypsin/protease activity and therefore has potential to effecti

83 en species production, consequently limiting protease activity and TLR9 signaling.

84 The effects of tetrapyrrole on NS3/4A protease activity and type I IFN induction were assessed

85 uring agents can alter digestion by reducing protease activity and/or substrate solubility, and addit

86 nged with Alternaria (with or without serine protease activity), and inflammation, remodeling, and lu

87 of proteases alone may not be indicative of protease activities, and new methods for measuring prote

88 uction of dead cell clearance, inhibition of protease activity, and dampening of inflammatory cell re

89 entation therapy still exhibit inflammation, protease activity, and elastin degradation that can be f

90 ct (CSE) and aeroallergens lacking intrinsic protease activity, and IL-6 and IL-8 production measured

91 ng modified the localization, acidification, protease activity, and proteomic profile of lysosomes.

92 tructural protein 3, which negatively affect protease activity, and valine residues 785 and 787, whic

93 reduction in body temperature and decreased protease activity; and (iii) a marked redistribution of

94 Acidic lysosomal pH and increased protease activity are essential for digestion.

95 Unregulated changes in protease activity are linked to many diseases including

96 nes Klf2 and Klf4, as well as Rho and ADAMTS protease activity, are increased in the endothelial cell

97 Strategies that coopt protease activity as molecular triggers are increasingly

98 By contrast, we treat protease activity as multi-valued (i.e., signal is betwe

99 RadA is unlikely to possess protease activity as the putative active site serine is

100 construct peptide-caged liposomes that treat protease activity as two-valued (i.e., signal is 0 or 1)

101 leavage by meprin beta caused increased ADAM protease activities, as observed by peptide-based cleava

102 A into picoliter-scale droplets of an HIV-1 protease activity assay to model ultraminiaturized compo

103 onstrate dose-response screening in an HIV-1 protease activity assay.

104 qRT-PCR and protease activity assays demonstrated that under standar

105 f meprin beta with ADAM proteases to control protease activities at the cell surface as part of the p

106 re subject to rapid degradation by excessive protease activity at the wound environment.

107 mis was dependent on bacterial viability and protease activity, because killed bacteria and a proteas

108 f H3K18 as a central regulator of MMP-9 H3NT protease activity both in vitro and at H3NT cleavage sit

109 2A mutants that retain protease activity but are unable to interact with SETD3

110 Gram-negative bacteria, possessed no general protease activity but cleaved gamma-links in both d- and

111 otably, recombinant mutant tPA-S478A lacking protease activity (but retaining the EGF-like domain) wa

112 ered mitochondrial morphology, and decreased protease activity, but epidemiologic studies of an assoc

113 rity, demonstrating that control of cysteine protease activity by CF is critical for normal eosinophi

114 e thiol reactivity but potently inhibit CatK protease activity by formation of an irreversible covale

115 ism, rotation of equivalent helices controls protease activity by movement of the equivalent carbonyl

116 The inhibition of protease activity by using a serine protease inhibitor l

117 proteins that respond immediately to reduced protease activity can be identified.

118 These results show that protease activity can be used to process biological info

119 Inhibition of protease activity can block RNA replication by preventin

120 ilm formation and suggest that extracellular protease activity can influence whether Aap contributes

121 vided time- and dose-dependent factor I-like protease activity capable of cleaving C3b into inactive

122 known mechanism whereby NiV provides a novel protease activity capable of in vitro cleavage and inact

123 In vitro, inactivation of MALT1 protease activity caused reduced stimulation-induced T c

124 Alternaria-specific serine protease activity causes rapid IL-33 release, which unde

125 d after FLG knockdown included inflammation, protease activity, cell structure, and stress.

126 We showed that Der p 1, which has cysteine protease activity, cleaves the ectodomain of peptidoglyc

127 This increased protease activity coincided with increased expression of

128 letion of ATG genes or inhibition of vacuole protease activity compromises Rph1 turnover.

129 anzymes" with interdependent ion channel and protease activity conferred by a single structural domai

130 In vivo, EspL cysteine protease activity contributes to persistent colonization

131 g ISGylation by specific inhibition of USP18 protease activity could constitute a promising antiviral

132 mature form, demonstrating that the cysteine protease activity critically contributes to the allergen

133 Results indicated that SUMO-protease activity decreased in a LMP1-dependent manner,

134 d to cytokinesis defects and cell death in a protease activity-dependent fashion.

135 The results show significant differences in protease activity depending on the assay used.

136 d syncytium formation, and endogenous serine protease activity did not contribute greatly to infectio

137 Here we found that HDMs with cysteine protease activity directly activated peptidergic nocicep

138 to a lack of methods to measure and localize protease activity directly within the tissue microenviro

139 that growth cones use invadosomes to target protease activity during axon guidance through tissues.

140 ine proteases and also others that have lost protease activity during evolution.

141 selective pressure to decrease secreted SpeB protease activity during infection.

142 ogether, these data indicate that inhibiting protease activity during polarized tumor cell 3D migrati

143 rovide a computational method for estimating protease activities efficiently by reducing the number o

144 Analysis of protease activity for the preferred residues at the clea

145 tention is permanent, inhibiting any further protease activity for the remainder of its life cycle.

146 to simultaneously measure multiple specific protease activities from water-in-oil droplets that cont

147 Some allergens with relevant protease activity have the potential to directly interac

148 ved (in conjunction with increased lysosomal protease activities) higher microtubule-associated prote

149 Loss of Ddi1 or its putative protease activity hypersensitizes cells to DPC trapping

150 hat the PRT6 branch of the pathway regulates protease activities in a complex manner and optimises st

151 into multiplex chips for rapid profiling of protease activities in cancer diagnosis and treatment mo

152 IHZ(TM) assay, for the detection of specific protease activities in situ.

153 chinery in immune cells harboring 3 distinct protease activities in the LMP2 (low-molecular-weight pr

154 Specific protease activities in the sample are then inferred from

155 in -Cout orientation and possesses ubiquitin protease activities in vitro.

156 trates showed high protease activity, whilst protease activity in 13 culture filtrate was low.

157 Serine protease activity in Aedes aegypti saliva augmented viru

158 nd, compared with SD therapy, reduced serine protease activity in BALF (elastase and cathepsin G), pl

159 orm for real-time, quantitative detection of protease activity in biological fluids.

160 se activities, and new methods for measuring protease activity in biological samples such as tumor bi

161 we find that PAR1 stimulation induces MALT1 protease activity in both osteosarcoma and breast cancer

162 r the simple detection and quantification of protease activity in buffer and human serum.

163 acellular cystatin C is linked to pathologic protease activity in cancer, arthritis, atherosclerosis,

164 loped luciferase-based biosensors to monitor protease activity in cells.

165 Elevated protease activity in cKO oviducts causes premature degra

166 ique can be used for real time monitoring of protease activity in crude preparations of virtually any

167 Detection of protease activity in diseased tissues could therefore be

168 This suggests a role for MALT1 protease activity in endothelial cells targeted by mast

169 ymography probes (AZP) detected dysregulated protease activity in human prostate cancer biopsy sample

170 eviously unappreciated key function of MALT1 protease activity in immune homeostasis and underline it

171 Additionally, monitoring of protease activity in individual virions distinguishes be

172 or SPPL3, indicating a requirement for SPPL3 protease activity in NK cell biology.

173 thesis is activated through control of ClpCP protease activity in response to signals of PG homeostas

174 udies identified changes in pigmentation and protease activity in response to YjbIH and are the first

175 o identify genes essential for extracellular protease activity in Serratia sp. strain SCBI and to det

176 fluorescence ratio, reliably predicted viral protease activity in single virions.

177 and 1457 were identical, implicating altered protease activity in the differential Aap processing res

178 linalool did not inhibit proteasome-related protease activity in the in vitro assays.

179 sured whether Wnt signaling led to increased protease activity in the joint.

180 n family, that likely acts as a regulator of protease activity in the parasite.

181 Plant N uptake was strongly coupled with protease activity in the rhizosphere.

182 ine residue 117, which markedly enhances its protease activity in vitro, is critical for its ability

183 trategy allows for non-invasive detection of protease activity in vivo and ex vivo by tracking deposi

184 the DNA-binding domain that is necessary for protease activity in vivo.

185 n keratinocytes to increase their endogenous protease activity, including specific increases in tryps

186 Whereas intracellular protease activity increased with nutrient stress, endocy

187 Unregulated protease activity is a biomarker of several human diseas

188 Thus, glucosyltransferase but not cysteine protease activity is critical for TcdB-mediated cytopath

189 o drive the cytokine response and that MALT1 protease activity is essential.

190 uronal XBP-1s are more acidic, and lysosomal protease activity is higher.

191 o test the therapeutic hypothesis that HtrA1 protease activity is involved in the progression of AMD.

192 Protease activity is measured by imaging the activated P

193 stemic dissemination, suggesting that fungal protease activity is not required for invasion during co

194 Protease activity is read out via proteolytic release of

195 We further demonstrate that the SUMO protease activity is required for supernumerous mitotic

196 Protease activity is required to generate viral nonstruc

197 After synthesis, protease activity is tightly controlled.

198 y in actinomycetes, exhibits both lipase and protease activities, is secreted into macrophages, and c

199 rin-dependent plasmin generation and reduced protease activity (Kcat/KM 2.57 +/- 1.02 x 10(-)(3) and

200 PMA treatment not only elevates the average protease activity level but also reduces the cellular he

201 Importantly, measuring protease activity levels, rather than concentrations, is

202 Reduction in mite protease activity limited its capacity to induce oxidati

203 In addition to its conventional papain-like protease activity, Lpro acts as a deubiquitinase (DUB) a

204 Thus, suppression of oviductal protease activity mediated by estrogen-epithelial ERalph

205 This platform was used to screen protease activities of a wide range of cell types, formi

206 Multiple protease activities of single cells harvested from a tum

207 iation, autophagosome to lysosome fusion, or protease activities of the lysosome and proteasome.

208 Notably, IRF7 cleavage strictly requires the protease activity of 3C(pro).

209 us (FMDV) 3C(pro) and that this requires the protease activity of 3C(pro).

210 lastin, the former of which protects against protease activity of allergens and the latter with a rol

211 The protease activity of Amb a 11, as well as its capacity t

212 Targeting protease activity of Aspergillus fumigatus in conditions

213 erically and negatively regulates the serine protease activity of calnuc, inhibition being caused by

214 A therapeutic agent that targets the ECM protease activity of damage-responsive lung fibroblasts

215 e site, in agreement with the tryptic serine protease activity of FVIIa.

216 ient for performing functional assays of the protease activity of individual leukocytes.

217 is developed to effectively detect secretory protease activity of individual viable leukocytes.

218 nsated for this effect by reducing the basal protease activity of nsP2.

219 tations specifically disrupting the cysteine protease activity of PEDV nsp5 abrogated NEMO cleavage a

220 Mechanistically, the cysteine protease activity of PEDV nsp5 mediates proteolysis of N

221 Protease activity of Per a 10 favours Th2 responses by d

222 Protease activity of Per a 10 increased p-STAT3 levels i

223 tease inhibitor SERPINB1 counterbalanced the protease activity of PR3 in aging neutrophils, and delet

224 These proteins are targets for the protease activity of purified DDI2.

225 The protease activity of S epidermidis isolates was compared

226 The protease activity of separase is strictly regulated by t

227 n of CRPC cell apoptosis independent of anti-protease activity of SLPI.

228 The pseudokinase domain might modulate the protease activity of SltB.

229 Thus, protease activity of the alphavirus capsid is a potentia

230 as well as deneddylation, facilitated by the protease activity of the CSN (COP9 signalosome), are req

231 Protease activity of the HDM extract was reduced to inve

232 y, we determined that the ubiquitin-specific protease activity of the ORF UL48 protein was functional

233 suppressant drugs, alpha-amylase protein, or protease activity of thrombin and Factor Xa.

234 Compared with the protease activity of wild-type caspase-9, that of Casp9-

235 d the effect of inhibiting allergen-specific protease activities on IL-33 levels was assessed.

236 tools, little spatiotemporal information on protease activities on NETs is available in a pathophysi

237 irus papain-like protease, altered the viral protease activity or affected viral replication or patho

238 Second, while not inhibiting the protease activity per se, ZDHHC5-mediated Furin/PC7 palm

239 sing these signatures to estimate individual protease activities primarily use an extensive collectio

240 one deacetylase (HDAC) and tumour-associated protease activities produced in malignant cancer cells.

241 We characterized protease activity profiles at single cell resolution for

242 To address the need for spatially resolved protease activity profiling in cancer, we developed a ne

243 We applied protease activity profiling with these new probes on Ara

244 ergic responses were dependent on subtilisin protease activity, protease-activated receptor-2, IL-33R

245 Finally, chemically blocking protease activity protected against mutant TDP-43(A315T)

246 ar stress, PI16 contributes to inhibition of protease activity; protection that can be reversed durin

247 s likely due to inhibition of ClpP-dependent protease activity rather than activation.

248 The protease activity reached its maximum at 40 degrees C in

249 Most assays to monitor protease activity rely on bulk analysis of millions of v

250 It is not understood how the protease activity required for rupture is directed with

251 gdorferi, annotated as BB0104, having serine protease activity responsible for the primary cleavage o

252 , so we hypothesized that LMP1 inhibits SUMO-protease activity, resulting in reduced de-sumoylation o

253 ed a sigma factor B (DeltasigB) mutant where protease activity results in a biofilm-negative phenotyp

254 Here, we characterize bacterial CE protease activities, revealing K63-linkage-specific deub

255 casein hydrolysis assays to measure the SpeB protease activity secreted by 6,775 GAS strains recovere

256 This new method of detecting protease activity shows superior performance to conventi

257 (PrAMA), we are able to infer six different protease activity signals from individual cells in a hig

258 tural amino acids can be used to investigate protease activities/specificities for peptides containin

259 on aeroallergens, possessed intrinsic serine protease activity that elicited the rapid release of IL-

260 ubjects, suggesting heterogeneity in Akt and protease activity that may play a role in the RA-affecte

261 small group of proteins, most with cysteine protease activity that target several key proteins invol

262 transmembrane pathway and also regulates the protease activity, thereby coupling substrate processing

263 in substrate and ECM interactions, fine-tune protease activity to a particular developmental context.

264 Reporting methods link protease activity to biochemical signals, whereas contro

265 ategy to block Streptococcus pneumoniae IgA1 protease activity to potentially prevent infection.

266 Here, we show that XopJ has protease activity to specifically degrade RPT6, leading

267 optode platform is used to indirectly detect protease activity (trypsin) based on proteolytic digesti

268 also established an in vitro assay for TIKI2 protease activity using FRET peptide substrates derived

269 ectrochemical biosensor for the detection of protease activity using self-assembled monolayers (SAMs)

270 chnique, a diverse profile of MMP and serine protease activities was characterized in breast cancer p

271 The pH optimum for protease activity was acidic (5 to 6) in the gut with th

272 Secreted protease activity was characterized from 44 ocular clini

273 The capacity for multiplexed sensing of protease activity was demonstrated using these two ortho

274 Protease activity was determined using fluorogenic activ

275 d by Hg(2+), Zn(2+) Co(2+) and Cu(2+), while protease activity was increased in the presence of Fe(2+

276 Protease activity was measured using fluorogenic activit

277 After activation, however, protease activity was pH-independent.

278 Tiki1 protease activity was shown to be metal ion-dependent an

279 To separate the DUB activity from the protease activity, we employed a structure-guided mutage

280 To evaluate the effect of the mutation on protease activity, we purified WT and Ubl mutant PLP2 an

281 Further to its protease activity, we show that AtLEGbeta exhibits a tru

282 Cathepsin K gene expression and protein and protease activity were detected in LAM-associated fibrob

283 ntrast, antimicrobial peptide production and protease activity were elevated in burn margin.

284 LAMP2 isoforms and proteasome and lysosomal proteases activities were unperturbed by LAMP-2C ectopic

285 ins were observed to produce strong cysteine protease activity when grown at high density.

286 ase is enigmatic, because its tetramers lack protease activity, whereas beta-tryptase tetramers are a

287 The C-terminal part of nsP2 possesses protease activity, whereas the N-terminal part exhibits

288 1 to 95) alone of NS2B is sufficient for NS3 protease activity, whereas the role of transmembrane dom

289 , in addition to p38 MAPK and PI3K, a serine protease activity, whereby FAP-alpha is the most likely

290 pain-like protease 2 (PLP2) domain possesses protease activity, which cleaves the viral replicase pol

291 concentrations and DEVDase (caspase-3 like) protease activity, which have been associated with PCD i

292 DPC processing through a unique DNA-induced protease activity, which is controlled by several sophis

293 Such a dual glycosyltransferase-protease activity, which occurs in the same active site,

294 libraries of peptide substrates that detect protease activity, which provides valuable biological in

295 arent WT and 5 culture filtrates showed high protease activity, whilst protease activity in 13 cultur

296 r bioanalytical techniques to rapidly detect protease activities with high sensitivity and high speci

297 4-week trial, reduction of neutrophil serine protease activity with brensocatib in patients with bron

298 applications of biochemical methods to track protease activity, with an emphasis on the use of activi

299 Further, we demonstrated elevated protease activity within the extracellular DNA of sputum

300 Visualization of protease activity within the native tissue context using