ライフサイエンスコーパス: protein array

1 s of proteinaceous samples (e.g., tissues or protein arrays).

2 0 with no-CAV were initially screened with a protein array.

3 genes in renal cancer cells, we performed a protein array.

4 antified by Western blot analysis, ELISA, or protein array.

5 odology called the nucleic acid-programmable protein array.

6 nalyzed by cDNA microarray and by a cytokine protein array.

7 ared to be composed of a highly cross-linked protein array.

8 by a transforming growth factor-beta (TGFB) protein array.

9 of 55 vasoactive proteins were measured with protein array.

10 n screen by incubating recombinant CHD7 on a protein array.

11 this strategy to the generation of periodic protein arrays.

12 ified by both nano-LC-ESI-MS/MS and antibody protein arrays.

13 e to ~170 drug compounds using reverse-phase protein arrays.

14 le means of generating high-density membrane protein arrays.

15 (S-layer), which is composed of crystalline protein arrays.

16 l-transduction pathways, using reverse-phase protein arrays.

17 htforward technique for fabricating membrane protein arrays.

18 been used only sporadically for high-content protein arrays.

19 in the HGFs conditioned media using cytokine protein arrays.

20 ous flow will help expand the application of protein arrays.

21 analysis can be adapted and applied to these protein arrays.

22 as well as in the fabrication of peptide and protein arrays.

23 ips and made important advances in preparing protein arrays.

24 in cytokines and chemokines were checked by protein arrays.

25 S-CoV-2 proteins, as measured by peptide and protein arrays.

26 gical ligands to afford one-dimensional (1D) protein arrays.

27 m 483 pediatric patients using reverse phase protein arrays.

28 bricated and tested, for the use in DNA- and protein arrays.

29 tro kinase selectivity screening and phospho-protein arrays.

30 and signaling molecules were measured using protein arrays.

31 Using antibody-based protein arrays, 328 cytokines, chemokines, and receptors

32 Western blot and reverse phase protein array analyses consistently demonstrated that PI

33 Using protein array analyses, we then found that ectopic expre

34 ified using RNA sequencing and reverse-phase protein array analyses.

35 Protein-array analyses identified host cell factors up-r

36 A protein array analysis identified IGF1 to be downstream

37 Here, protein array analysis identified the SUMO E3, protein i

38 Reverse phase protein array analysis linked active Hck and Fgr to the

39 Reverse-phase protein array analysis of phospho-proteomic changes in m

40 Reverse-phase protein array analysis of PKCiota wild-type, catalytic a

41 Cytokine protein array analysis revealed that the inhibitory acti

42 Reverse phase protein array analysis suggested additional differences

43 Phosphoproteomic profiles from reverse-phase protein array analysis supported by mRNA profiling ident

44 6 and the key proresolving enzyme Alox-12/15 Protein array analysis, as well as immune cell phenotype

45 Through reverse phase protein array analysis, we demonstrate that ZNF304 promo

46 tudied by Western blotting and reverse-phase protein array analysis.

47 th separate datasets including reverse-phase protein array and an in vivo shRNA dropout screen.

48 ore, 53 angiogenic factors were examined via protein array and compared between ODN1826- and water-tr

49 ients using a validated robust reverse-phase protein array and correlated ARC levels with clinical ou

50 teractions using a nucleic acid programmable protein array and discovered that Rac1 binds to the prot

51 ammatory biomarkers assessed by a customized protein array and enzyme assay.

52 4, CCL5, CXCL9, and CXCL10) was confirmed by protein array and/or quantitative reverse transcription-

53 ectrometric analysis, and the development of protein arrays and biochips.

54 transcriptional profiling, and reverse-phase protein arrays and by sequencing for common breast cance

55 The combinations of protein arrays and conditioned medium arrays or serum ar

56 ession of 54 transcription factors using DNA/protein arrays and electrophoretic mobility shift assays

57 for the facile production of highly ordered protein arrays and for electron density mapping in struc

58 ungal species, and novel approaches, such as protein arrays and genomic deletion libraries, are being

59 esearch describes how to prepare immobilized protein arrays and how to assay the binding of labeled t

60 domains as a probe, we screened high-density protein arrays and identified five candidate-binding par

61 Reverse-phase protein arrays and immunohistochemistry, respectively, d

62 d lipid autacoid profiles were determined by protein arrays and mass spectrometry-based lipidomics.

63 els were evaluated using Q-PCR, angiogenesis protein array, and Western blotting.

64 gh-throughput analysis by similarity search, protein arrays, and affinity chromatography revealed fou

65 atile mass spectrometry-based techniques and protein arrays, and encourage further proteomics researc

66 utism, we used immunocytochemistry, cytokine protein arrays, and enzyme-linked immunosorbent assays t

67 t of hAH was also analyzed by antibody-based protein arrays, and selected proteins were quantified.

68 combination of RNAi screening, reverse phase protein arrays, and small molecules testing, we identify

69 an be directly immobilized for biosensor and protein array applications.

70 Protein arrays are a high-throughput approach for proteo

71 DNA and protein arrays are commonly accepted as powerful explora

72 Protein arrays are described for screening of molecular

73 a core constituent of a linear multilayered protein array at the plasma membrane, expected for an NP

74 In addition, a reverse-phase protein array-based analysis and subsequent loss-of-func

75 actions, we surveyed the human proteome by a protein array-based approach and identified 671 proteins

76 iscovery mass spectrometry and reverse-phase protein array-based proteomics dual approach was used to

77 ry can be effectively used to identify plant proteins arrayed by two-dimensional gel electrophoresis.

78 At least 20 copies of a protein array can be obtained from a single DNA array.

79 Such studies show that protein arrays can be used to examine in parallel the fu

80 ity of creating mutant proteins to achieve a protein array capable of detecting drugs of abuse in sol

81 In parallel, targeted proteomic analysis by protein array categorized the resistant samples into 3 s

82 We constructed a viral protein array comprising the complete proteomes of seven

83 An unbiased protein array confirmed this reduction in ERK and Src ac

84 In addition, His-tagged fusion protein arrays created on gold surfaces were also used t

85 andscape via flow cytometry, RNA sequencing, protein arrays, culture assays, and spatial tissue chara

86 We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of rep

87 exome sequencing (n = 374) and reverse-phase protein array data (n = 212) from head and neck squamous

88 mic regulations by integrating reverse phase protein array data and the stiffness associated pathway.

89 mic regulations by integrating reverse-phase protein array data and the stiffness-associated pathway.

90 ex disease datasets including microarray and protein array data demonstrate that the proposed POC-bas

91 Analysis of protein array data demonstrated lower apoptosis-associat

92 ation, microRNA expression and reverse-phase protein array data for 1,072 cell lines from individuals

93 In vivo, reverse phase protein array data for tumors revealed that basal levels

94 tography-mass spectrometry and reverse phase protein array data from human MM cell lines with computa

95 Analysis of reverse-phase protein array data indicated that increased expression o

96 However, protein array data may not fully capture pathway activit

97 methylation data together with reverse-phase protein array data on a panel of 68 highly annotated ear

98 Reverse-phase protein array data show that PEA15 levels in invasive br

99 n breast cancer microarray and reverse-phase protein array data was performed to identify expression

100 trols) in 42 malaria (Plasmodium falciparum) protein arrays derived from 22 donors with various clini

101 Protein arrays detected several angiogenesis-related fac

102 A), which allows the 'printing' of replicate protein arrays directly from a DNA array template using

103 New technologies in protein arrays, either on chips or with self-encoded ele

104 Phospho-protein arrays elucidated the activation of protein kina

105 cinoma, humoral immune response, antibodies, protein array, endemicity.

106 f Treponema pallidum subsp. pallidum using a protein array enzyme-linked immunosorbent assay.

107 alloantibodies was performed with the use of protein arrays, enzyme-linked immunosorbent assays, and

108 eling by combining computer simulations with protein array experiments and electron microscopic imagi

109 Combined yeast two-hybrid and protein array experiments demonstrated that the TRD3 bin

110 Saliva was analyzed using a multiplex protein array for 30 biomarkers associated with host def

111 e report the development of a hydrogel-based protein array for quantitative and reproducible determin

112 Saliva and serum were analyzed with protein arrays for 14 pro-inflammatory and bone turnover

113 This method can be used to produce protein arrays for chip-based assays and DNA arrays for

114 ill be useful in the construction of regular protein arrays for structural analysis, in the construct

115 Protein arrays for this study were made by expressing bo

116 Here we developed a reverse-phase protein array from 4126 overexpressed E. coli clones, ly

117 ties following antibody functionalization on protein arrays from undiluted human plasma and indicates

118 array) and NAPPA (nucleic acid programmable protein array) from DNA and puromycin-mediated immobilis

119 Reverse phase protein array further demonstrated activation of noncano

120 e crucial issue of design and fabrication of protein arrays have been addressed in several studies, b

121 Cell and protein arrays have demonstrated remarkable utility in t

122 d high-through-put technologies like DNA and Protein arrays have resulted in a very large amount of i

123 Protein arrays hold great promise for proteome-scale ana

124 s to yield different organized transmembrane protein arrays: (i) a bacteriorhodopsin purple membrane-

125 Screening by chemokine protein array identified a subset of melanoma cell lines

126 A protein array identified upregulation of fatty acid-bind

127 These assays with protein arrays identify MS patients with a highly active

128 otein profiling analysis using reverse phase protein array; ii) computationally developed an Integer

129 that immobilize the probe, our reverse phase protein array immobilizes the whole repertoire of patien

130 ne deacetylation were assessed using reverse protein array, immunoblotting, and chromatin immunopreci

131 This simple method for creating protein arrays in combination with our analyses of sever

132 dent proteins were measured by Reverse Phase Protein Arrays in hippocampus and cortex, and 64 in cere

133 ce-based protein detection limits the use of protein arrays in research and clinical diagnosis.

134 important autoantibody targets identified on protein arrays included Myc-associated zinc finger prote

135 onal proteomics resource using reverse phase protein arrays, incorporating data from nearly 8000 pati

136 Using a protein array, it was determined that LMP1 selectively a

137 The assembled protein arrays maintain structural stability and biologi

138 titatively assess the quality of gel element protein arrays manufactured with whole-cell lysate in vi

139 ose a metadata format known as reverse phase protein array markup language (RPPAML).

140 s such as laser-capture mass spectrometry or protein arrays may be helpful as well.

141 l approach to designing two-dimensional (2D) protein arrays mediated by noncovalent protein-protein i

142 Using the yeast two-hybrid system and a protein array membrane, we identified two novel protein

143 In situ or on-chip protein array methods use cell free expression systems t

144 rofluidic-based comprehensive human membrane protein array (MPA).

145 mary liver cancer tissues with reverse-phase protein arrays, mutational analysis using whole genome s

146 rray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of p

147 dogs using a novel nucleic acid programmable protein array (NAPPA) platform.

148 s fabricated using nucleic acid programmable protein array (NAPPA) technology, which enables the high

149 In one approach, we constructed a protein array of about 6,000 yeast transformants, with e

150 ur method has the advantage over traditional protein arrays of being able to detect autoimmunity to e

151 Importantly, an independent analysis of protein arrays of human colon tumors colonized with SGG

152 Protein arrays of liver homogenates from patients with a

153 escence and analyzed angiogenic molecules by protein arrays of lysates and cell culture supernatants.

154 Nevertheless, the proteins arrayed on this lattice necessarily have an int

155 icle, we describe the fabrication in situ of protein arrays optimized for MALDI characterization.

156 s vasoactive protein levels were measured by protein array or enzyme-linked immunosorbent assay.

157 terations, typically by using reversed-phase protein arrays or mass spectrometry, has lagged, however

158 cteria and archaea possess a two-dimensional protein array, or S-layer, that covers the cell surface

159 ces such as electronic biosensors, thin-film protein arrays, or biofuel cells.

160 ral bias in the muscovite lattice to produce protein arrays ordered over tens of millimetres.

161 Additionally, reverse phase protein array pathway activation mapping profiled the si

162 nstrate the potential to use the multiplexed protein array platform for respiratory disease diagnosis

163 oma, we used a high-throughput reverse-phase protein array platform to identify signaling alterations

164 For protein arrays, potential advantages of this format are

165 Employing a protein array produced in vitro from 1,292 DNA fragments

166 cing, immunohistochemistry and reverse phase protein array profiling (RPPA) on OS specimens from 48 p

167 ds whole genome sequencing and reverse-phase protein array profiling data, which can be correlated wi

168 ges in key enzymes measured by reverse phase protein array profiling.

169 The protein array provided a sensitive platform for global s

170 ys, but also for other technologies, such as protein arrays, quantitative mass spectrometry and next-

171 d Pf-specific antibody profiles, measured by protein arrays representing the full Pf proteome, of 40

172 mes were characterized by RNA sequencing and protein array, respectively.

173 as measured by gene expression and cytokine protein array, respectively.

174 A reverse-phase protein array revealed that HuR-mediated regulation of P

175 Reverse-phase protein array revealed that the Ras-dependent downstream

176 Subsequent non-biased protein array revealed that these hepatocytes subjected

177 Hybridization to a protein array revealed the cross-reactive protein LIM-do

178 Our screening, using a reverse-phase protein array, revealed distinct mechanisms by which PAK

179 nic mice by RNA sequencing and reverse phase protein array reveals modulation of oncogenic networks o

180 Further, reverse phase protein array (RPPA) analysis demonstrated that IFN-gamm

181 Reverse-phase protein array (RPPA) analysis in UVM cells treated with

182 the Braf-mutant cell lines by reverse phase protein array (RPPA) analysis showed no significant asso

183 Reverse phase protein array (RPPA) analysis was performed in the same

184 ptional response by exploiting reverse phase protein array (RPPA) and mRNA expression data in The Can

185 xpression using immunoblot and reverse phase protein array (RPPA) and then subjected to Ingenuity Pat

186 We developed a reverse phase protein array (RPPA) based phosphor-antibody characteriz

187 Reverse-Phase Protein Array (RPPA) is a robust high-throughput, cost-e

188 transcriptomic, proteomic, and reverse phase protein array (RPPA) measurements established low basal

189 tudy, we performed an unbiased Reverse Phase Protein Array (RPPA) on TNBC cells treated with chemothe

190 g with a functional proteomics reverse-phase protein array (RPPA) platform assessing 42 phospho and t

191 High-throughput reverse-phase protein array (RPPA) technology allows for the parallel

192 In this study, we used reverse phase-protein array (RPPA) technology to measure changes in mu

193 Reverse-phase protein array (RPPA) technology uses panels of high-spec

194 types using the antibody based reverse phase protein array (RPPA) technology.

195 after radiation, we utilized a reverse phase protein array (RPPA) to identify significant changes in

196 Reverse phase protein array (RPPA) was performed on 205 proteins isola

197 Reverse phase protein array (RPPA) was performed to assess multiple on

198 129 ALL patient samples using reverse phase protein array (RPPA) with ErbB2 and phospho-ErbB2 antibo

199 ple other platforms, including reverse phase protein array (RPPA), miRNA, copy number variation (CNV)

200 report a novel functionalized reverse phase protein array (RPPA), termed polymer-based reverse phase

201 ing, immunohistochemistry, and reverse phase protein array (RPPA), we aim to understanding the role o

202 ion or knock-down, employing a reverse-phase protein array (RPPA).

203 In this study, Reverse Phase Protein Arrays (RPPA) and targeted mass spectrometry ass

204 Reverse Phase Protein Arrays (RPPA) are convenient assay platforms to

205 Reverse phase protein arrays (RPPA) measure the relative expression le

206 Reverse-phase protein arrays (RPPA) represent a powerful functional pr

207 Utilizing reverse phase protein arrays (RPPA) to assess the proteome and explore

208 ncing, DNA methylation arrays, reverse phase protein arrays (RPPA), microRNA, and somatic mutational

209 ity extension assay (PEA), and reverse phase protein arrays (RPPA).

210 Here, we describe the use of Reverse Phase Protein Arrays (RPPAs or RPLAs) to profile signaling pro

211 Reverse phase protein arrays (RPPAs) are a powerful high-throughput to

212 A reverse-phase protein array screen identified SRC3/AIB1 as one oncopro

213 In a protein array screen, we identify a cluster of mitotic e

214 Using a ubiquitin binding domain protein array screen, we identify that the UBA domains o

215 identified LANA-interacting proteins using a protein array screen.

216 Using high-throughput protein array screening and bioinformatic analysis, we i

217 ptor (BCR) expression cloning and subsequent protein array screening as a frequent and proliferation-

218 We also used Reverse Phase Protein Array screening to identify differential protein

219 Using protein array screening, we showed PTTG1 interacting wit

220 ly specific antibody-antigen interactions by protein array screening.

221 gingivalis and analyzed by a phosphorylation protein array show a reduction of Akt only in BMMPhi fro

222 converting only part of the DNA array into a protein array, simultaneous detections of DNA and protei

223 With the subsequent design of SPR protein arrays, SPR-MS is expected to enter into the fie

224 Unbiased reverse phase protein array studies and subsequent validation experime

225 Reverse phase protein array suggested that high expression of the esse

226 g of multiple cytokine expression based on a protein array system.

227 should allow us to develop many high-density protein array systems to detect a variety of proteins.

228 up-regulation of PKCbeta protein in WM using protein array techniques and immunohistochemistry.

229 When coupled with new protein array technologies, high-throughput SPR methods

230 strategies should increase the usefulness of protein array technologies.

231 e, a robust strategy comparable with gene or protein array technology for comprehensively analyzing t

232 We have developed a multianalyte fluid-phase protein array technology termed high-throughput immunoph

233 ies (autoAbs) in PV and (ii) use multiplexed protein array technology to define the scope and specifi

234 We used protein array technology to identify the MAP3K2 kinase a

235 Using protein array technology, we identified an E3 ubiquitin

236 These methods address three issues in protein array technology: (i) efficient protein expressi

237 We describe a format for production of protein arrays termed 'protein in situ array' (PISA).

238 e responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptid

239 re spanned by asymmetric membrane-associated protein arrays that had approximately 6-nm lateral perio

240 In contrast to previous protein arrays that immobilize the probe, our reverse ph

241 Protein arrays that measure multiple protein cancer biom

242 Thus, instead of a holey protein array, the experimental model presented here app

243 biochemical activities, the construction of protein arrays, the identification of interactions, and

244 We first identified, by reverse-phase protein arrays, the lymphocyte cell-specific protein-tyr

245 Reverse phase protein array, tissue microarray, and quantitative flow

246 We generated an Epstein-Barr virus (EBV) protein array to evaluate the targets of the EBV protein

247 Here we used a planar protein array to investigate CNS-reactive antibodies in

248 pe-resolved metabolomics with reversed phase protein array to map the dynamic changes of the IM metab

249 We used reverse-phase protein array to measure the transient changes in the ph

250 ctroscopy-based sequencing and reverse-phase protein arrays to 547 human breast cancers and 41 cell l

251 Here we use reverse-phase protein arrays to analyse 3,467 patient samples from 11

252 tributed or pre-arrayed DNA or RNA, enabling protein arrays to be created on demand.

253 g to those variants were tested by ELISA and protein arrays to confirm their IgM-binding capacity.

254 are now being developed for construction of protein arrays to detect the presence of antibodies agai

255 italized on recently established designed 2D protein arrays to ectopically engineer cortical polarity

256 We also use reverse-phase protein arrays to generate comprehensive, quantitative S

257 loratory Stage I we utilized high-throughput protein arrays to identify 35 high-interest proteins sho

258 Here, we used high-density protein arrays to identify non-HLA antibodies in CAI and

259 We describe the use of protein arrays to identify proteins that bind to DNA seq

260 Here, we used high-density protein arrays to identify target antigens for AECAs iso

261 Here we develop three protein arrays to measure IgG autoantibodies associated

262 ts were also used with the His-tagged fusion protein arrays to study multiple antibody-antigen bindin

263 We have used protein arrays to test 492 pairings of a nearly complete

264 Here I report the development of a universal protein array (UPA) system that provides a sensitive, qu

265 Existing approaches to quantify protein arrays use parametric response curves fit to dil

266 We report the fabrication of a patterned protein array using three orthogonal methods of immobili

267 characterization of histidine-tagged fusion protein arrays using nitrilotriacetic acid (NTA) capture

268 proach to infer spatial relationships within protein arrays using stimulated emission depletion nanos

269 It was designed for the production of protein arrays using the ion soft-landing technique to c

270 In addition, the GST-Eps15 protein array was capable of detecting inhibition of EGF

271 A protein array was performed on NOD2-activated enterocyte

272 -mediated viral suppression, a reverse-phase protein array was used to broadly survey cellular signal

273 Using an unbiased reverse-phase protein array, we detected phospho-activation of heat sh

274 f the proteins screened in the reverse-phase protein array, we found that insulin receptor substrate

275 Utilizing a reverse-phase protein array, we observe rapid phospho-activation of hu

276 y, with the use of microfluidic networks and protein arraying, we demonstrate the potential of this c

277 Here, using oligonucleotide and protein arrays, we analyze the infection of primary pola

278 Using reverse-phase protein arrays, we generated ovarian carcinoma protein e

279 Using high density protein arrays, we have previously identified in vitro s

280 By using protein arrays, we identified a small molecule that bind

281 Using reverse-phase protein arrays, we measured expression levels of approxi

282 By screening sera with protein arrays, we showed that our treatment can induce

283 enitor cells using a validated reverse-phase protein array; we correlated its levels with clinical ou

284 All binding interactions occurred at 5x4 protein array were real-time monitored simultaneously.

285 Periodic protein arrays were achieved by templated self-assembly

286 Oriented protein arrays were fabricated using a two-step process:

287 In conclusion, protein arrays were used to identify a novel SPTLC1-Par3

288 Reverse-phase protein array, Western blot, and IHC were performed to e

289 ound healing assay, as well as reverse-phase protein arrays, western blot and immunofluorescence stai

290 more than 30 identical copies of a membrane protein array while requiring only femtomoles of protein

291 nosis of MAP infection, we constructed a MAP protein array with 868 purified recombinant MAP proteins

292 teraction motif, and by screening PDZ domain protein arrays with an SPTLC1 C-terminal peptide, we fou

293 The CFM can be used to deposit protein arrays with good spot morphology, spot-to-spot u

294 ts these voxels into biologically functional protein arrays with prescribed and ordered two-dimension

295 The existence of protein arrays, with a large selection of different kind

296 trates with equipment commonly used to print protein arrays, without complex surface chemistry protoc