ライフサイエンスコーパス: protospacer adjacent motif

1 exts and reduces restrictions imposed by the protospacer adjacent motif.

2 indow of the RNA:DNA hybrid, neighboring the protospacer adjacent motif.

3 e absence of the canonical NGG sequence as a protospacer-adjacent motif.

4 ands and recognizes the 5'-NNNVRYM-3' as the protospacer-adjacent motif.

5 e lacking tracrRNA, and it utilizes a T-rich protospacer-adjacent motif.

6 to the 12-bases proximal to the guide strand protospacer-adjacent motif.

7 tospacer immediately following the essential protospacer-adjacent motif.

8 use SpCas9 variants compatible with non-NGG protospacer adjacent motifs.

9 of pathological mutations with non-canonical protospacer-adjacent motifs.

10 within the activating target RNA (rPAM [RNA protospacer-adjacent motif]).

11 s assay, we provide direct evidence that the protospacer adjacent motif along with the first base of

12 argets via protein-mediated recognition of a protospacer adjacent motif and complementary base pairin

13 These systems are compatible with expanded protospacer adjacent motif and high-fidelity Cas9 varian

14 I systems, type III systems do not require a protospacer adjacent motif and target nascent RNA associ

15 eal how the effector complexes recognize the protospacer adjacent motif and target-strand DNA to form

16 uided endonuclease that recognizes 5' T-rich protospacer adjacent motifs and creates staggered double

17 vided sequence, with user-specified types of protospacer adjacent motif, and number of mismatches all

18 ry screening assay for SpCas9 binding to the protospacer adjacent motif, and used these assays to scr

19 trates that contain mismatches distal to the protospacer adjacent motif are stabilized by reorganizat

20 ecognize specific target sequences without a protospacer adjacent motif, but their lack of intrinsic

21 AsCpf1 recognizes the 5'-TTTN-3' protospacer adjacent motif by base and shape readout mec

22 volution so as to alter the recognition of a protospacer adjacent motif by the Cas1-Cas2 complex, whi

23 targeted mutagenesis at 16 possible NGN PAM (protospacer adjacent motif) combinations in duplicates.

24 rthologue complex targeting genes within the protospacer-adjacent motif discriminated between homozyg

25 9 nickase, expands the editing window at the protospacer adjacent motif-distal end and outperforms AB

26 fectors acquired an ability to recognize the protospacer-adjacent motif-distal end of the guide RNA-t

27 shed a new editor variant recognizing an NAA protospacer adjacent motif, expanding the base editing p

28 binding sequence, a Cas12a CRISPR array, and protospacer adjacent motif-flanked Cas12a target sequenc

29 tion machinery by selecting spacers from the protospacer adjacent motif-flanked DNA(2,3).

30 ivery, collectively offer compatibility with protospacer-adjacent motifs for editing approximately 82

31 ation of Cas nuclease activity, specificity, protospacer adjacent motif frequency and scission profil

32 ese mutations into sgRNA sequences (near the protospacer-adjacent motif ["near the PAM"]) or by targe

33 We find that the protospacer adjacent motif (PAM) affects primarily the R

34 type I-E CRISPR-Cas system, with a 5'-AAA-3' protospacer adjacent motif (PAM) and a 61-nucleotide gui

35 udies have highlighted the importance of the protospacer adjacent motif (PAM) and a proximal 8-nucleo

36 that the S. aureus Cas9 recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA

37 leaves double-stranded DNA targets bearing a protospacer adjacent motif (PAM) and complementarity to

38 9 cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to

39 Targeting requires a protospacer adjacent motif (PAM) and crRNA-DNA complemen

40 cell-free biochemical screens to assess the protospacer adjacent motif (PAM) and guide RNA (gRNA) re

41 It cleaves DNA with a 5'-NNNCC-3' Protospacer Adjacent Motif (PAM) and is sensitive to its

42 fied by guide RNA molecules and flanked by a protospacer adjacent motif (PAM) and is widely used for

43 Cas4 selects prespacers containing a protospacer adjacent motif (PAM) and removes the PAM pri

44 Mutations in the protospacer adjacent motif (PAM) and seed regions block

45 sed immunity mainly through mutations in the protospacer adjacent motif (PAM) and seed regions.

46 base conversion at positions proximal to the protospacer adjacent motif (PAM) and the A/C simultaneou

47 guide RNA but also require recognition of a protospacer adjacent motif (PAM) by the Cas9 protein.

48 With no protospacer adjacent motif (PAM) constraints and featuri

49 able nuclease for selectively processing the protospacer adjacent motif (PAM) containing prespacers t

50 gitidis (NmCas9) recognizes a 5'-NNNNGATT-3' protospacer adjacent motif (PAM) different from those re

51 CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-pr

52 er-present constraint: the requirement for a protospacer adjacent motif (PAM) flanking each target.

53 on strictly requires the presence of a short protospacer adjacent motif (PAM) flanking the target sit

54 tems require the presence of a trinucleotide protospacer adjacent motif (PAM) for efficient interfere

55 e trimming of prespacers and the cleavage of protospacer adjacent motif (PAM) in several type I CRISP

56 1) is limited by their requirement of a TTTV protospacer adjacent motif (PAM) in the DNA substrate.

57 Cas9-mediated cleavage requires a protospacer adjacent motif (PAM) juxtaposed with the DNA

58 of the DNA target sequence requires a short protospacer adjacent motif (PAM) located outside this se

59 Moreover, the requirement of a protospacer adjacent motif (PAM) nearby the mutation sit

60 However, their dependence on a 5'-TTTV-3' protospacer adjacent motif (PAM) next to DNA target sequ

61 The presence of a specific protospacer adjacent motif (PAM) next to the DNA target

62 e, which strictly requires the presence of a protospacer adjacent motif (PAM) next to the target site

63 ition by all studied Cas9 enzymes requires a protospacer adjacent motif (PAM) next to the target site

64 , single-nucleotide mutations in the seed or protospacer adjacent motif (PAM) of the target sequence

65 ated gene editing is recognizing a preferred protospacer adjacent motif (PAM) on target DNAs by the p

66 cific manner, dependent on the presence of a Protospacer Adjacent Motif (PAM) on the target.

67 KMM520 (PtrCAST) was characterized without a protospacer adjacent motif (PAM) preference which can ac

68 nucleases and find that they have different protospacer adjacent motif (PAM) preferences and the M44

69 ight a proofreading mechanism beyond initial protospacer adjacent motif (PAM) recognition and RNA-DNA

70 The strict protospacer adjacent motif (PAM) requirement hinders app

71 Cas9s with simple protospacer adjacent motif (PAM) requirements are partic

72 Characterizing the protospacer adjacent motif (PAM) requirements of differe

73 Due to protospacer adjacent motif (PAM) requirements, CRISPR/Ca

74 but have limited target ranges due to their protospacer adjacent motif (PAM) requirements.

75 he simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements.

76 s, including the crucial role of an extended protospacer adjacent motif (PAM) sequence and the impact

77 d mechanisms for the recognition of the GGTT protospacer adjacent motif (PAM) sequence and the struct

78 A protospacer adjacent motif (PAM) sequence flanking targe

79 genome requires the presence of a 5'-NGG-3' protospacer adjacent motif (PAM) sequence immediately do

80 y, thereby eliminating the requirement for a protospacer adjacent motif (PAM) sequence in the target.

81 a Cas9 nickase that is not constrained by a protospacer adjacent motif (PAM) sequence requirement.

82 ither display low activity or require a long protospacer adjacent motif (PAM) sequence, limiting thei

83 try populations (MAF 4.5%) that introduces a protospacer adjacent motif (PAM) sequence.

84 However, base editors are restricted by protospacer adjacent motif (PAM) sequences and specific

85 Cas9 by recognising a series of alternative protospacer adjacent motif (PAM) sequences while ignorin

86 g specificity from protein-DNA contacts with protospacer adjacent motif (PAM) sequences, in addition

87 nucleoprotein gene, two CRISPR RNAs without protospacer adjacent motif (PAM) site limitation are int

88 At NGG protospacer adjacent motif (PAM) sites, ABE8s result in

89 DNA immediately downstream from a 5'-CCN-3' protospacer adjacent motif (PAM) that is critical for in

90 ered by the requirement for an extended TTTV protospacer adjacent motif (PAM)(2).

91 t spacers are acquired from DNA flanked by a protospacer adjacent motif (PAM)(5,6) and inserted into

92 equently restricted by the requirement for a protospacer adjacent motif (PAM), and selecting the opti

93 The hypercompact size, T-rich minimal protospacer adjacent motif (PAM), and wide range of work

94 ) DNA targets near a short sequence termed a protospacer adjacent motif (PAM), Cas9 and Cas12 offer u

95 res a specific nucleotide sequence, called a protospacer adjacent motif (PAM), for target recognition

96 nition of a short DNA sequence, known as the protospacer adjacent motif (PAM), next to and on the str

97 ed to recognize altered DNA sequences as the protospacer adjacent motif (PAM), thereby expanding the

98 lele-selective CRISPR/Cas9 strategy based on Protospacer Adjacent Motif (PAM)-altering SNPs to target

99 Regulation occurs through a protospacer adjacent motif (PAM)-dependent interaction o

100 lleviated by either artificially melting the protospacer adjacent motif (PAM)-distal duplex or provid

101 t single-nucleotide polymorphisms and enable protospacer adjacent motif (PAM)-flexible DNA cleavage w

102 Accordingly, we found that our protospacer adjacent motif (PAM)-free CRISPR/Cas12a-assi

103 Herein, we developed a novel protospacer adjacent motif (PAM)-free loop-mediated isot

104 because the AcrIIA11:SaCas9 complex binds to protospacer adjacent motif (PAM)-rich off-target sites,

105 ct and process DNA for integration using the protospacer adjacent motif (PAM).

106 end on the fourth nucleotide upstream of the protospacer adjacent motif (PAM).

107 unction of distance and orientation from the protospacer adjacent motif (PAM).

108 e distal nucleotides, plus disruption of the protospacer adjacent motif (PAM).

109 A reveal that Cascade recognizes an extended protospacer adjacent motif (PAM).

110 ze is constrained by the need for a specific protospacer adjacent motif (PAM).

111 trinucleotide signature sequence called the protospacer adjacent motif (PAM).

112 require recognition of a short trinucleotide protospacer adjacent motif (PAM).

113 ain responsible for the interaction with the protospacer adjacent motif (PAM).

114 cleotide seed region in the sgRNA and an NGG protospacer adjacent motif (PAM).

115 entiate the single allele differences in NGG protospacer adjacent motifs (PAM sequence).

116 ding the well-studied Cas9 proteins, evolved protospacer-adjacent motif (PAM) and guide RNA interacti

117 he availability of Cas9 variants with varied protospacer-adjacent motif (PAM) compatibilities, some g

118 requirement for Cas proteins to recognize a protospacer-adjacent motif (PAM) in DNA target sites.

119 Furthermore, the protospacer-adjacent motif (PAM) in some Cas9 enzymes ca

120 CRISPR enzymes require a protospacer-adjacent motif (PAM) near the target cleavag

121 ity, including a further optimization of the protospacer-adjacent motif (PAM) of Streptococcus pyogen

122 iting, but the strict requirement for an NGG protospacer-adjacent motif (PAM) sequence immediately ne

123 engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing G

124 and engineered Cas9 variants with different protospacer-adjacent motif (PAM) specificities to expand

125 eered SpCas9 enzymes and characterized their protospacer-adjacent motif (PAM)(7) requirements to trai

126 and inserts into the binding pocket for the protospacer-adjacent motif (PAM), a short DNA sequence g

127 nput query sequences, it searches gRNA by 3' protospacer-adjacent motif (PAM), and possible off-targe

128 ssing of a crRNA guide, recognizes a 5'-TTN' protospacer-adjacent motif (PAM), and stably binds a gui

129 ection of genomic SNPs without requiring the protospacer-adjacent motif (PAM), as Cas12b requires PAM

130 upon introduction of mismatches proximal to protospacer-adjacent motif (PAM), demonstrating that mis

131 PR-Cas enzymes requires the recognition of a protospacer-adjacent motif (PAM), limiting target site r

132 Activation does not require a canonical protospacer-adjacent motif (PAM), nor is utilization of

133 a ~20-base-pair DNA sequence next to a short protospacer-adjacent motif (PAM).

134 ays dictated by the presence or absence of a protospacer-adjacent motif (PAM).

135 rice genomic sites which are followed by the protospacer-adjacent motif (PAM).

136 not require targets to contain any specific protospacer-adjacent motifs (PAM); is a multi-turnover e

137 NA flexibility at the region adjacent to the protospacer-adjacent-motif (PAM) contributes to Cas12a t

138 three CRISPR loci for which the identity of protospacer adjacent motifs (PAMs) was unknown until now

139 These systems have distinct protospacer adjacent motifs (PAMs), including AT-rich mo

140 iting can be limited by a lack of compatible protospacer adjacent motifs (PAMs), insufficient on-targ

141 CRISPR-Cas system recognizes a unique set of protospacer adjacent motifs (PAMs), which requires ident

142 RISPR-Cas9 or CRISPR-Cas12 nucleases require protospacer adjacent motifs (PAMs).

143 irement of Cas enzymes to recognize specific protospacer adjacent motifs (PAMs).

144 enome-wide including creating and destroying protospacer adjacent motifs (PAMs).

145 re remarkably diverse, they commonly rely on protospacer-adjacent motifs (PAMs) as the first step in

146 anisms of action, where most systems rely on protospacer-adjacent motifs (PAMs) for DNA target recogn

147 AV] vectors), off-target editing, or complex protospacer-adjacent motifs (PAMs) that restrict the den

148 Cas9 departure and repair factor loading at protospacer adjacent motif-proximal genomic DNA.

149 e (termed AiEvo2) for increased specificity, protospacer adjacent motif recognition, and efficacy on

150 hlight residues important in DNA binding and protospacer adjacent motif recognition.

151 reveals critical interactions necessary for protospacer-adjacent motif recognition and R-loop format

152 occus canis Cas9 that exhibits more flexible protospacer-adjacent motif recognition than the traditio

153 equires that the target sequence satisfy the protospacer adjacent motif requirement of the Cas9 domai

154 re much more flexible in their guide RNA and protospacer-adjacent motif requirements compared with mo

155 n screening using a base editor with relaxed protospacer-adjacent motif requirements(9) (NG versus NG

156 mation under Cas9 binding, the effect of the protospacer adjacent motif sequence, and the folding sta

157 nonheritable manner and is not restricted by protospacer adjacent motif sequence.

158 as9 proteins is governed by binding first to protospacer adjacent motif sequences on DNA, which is fo

159 bp insertions matching the nucleotide on the protospacer-adjacent motif side of the break, a variable

160 deletions with junctions that do not fall at protospacer-adjacent motif sites.

161 Because of its distinct protospacer adjacent motif, the N. meningitidis CRISPR-C

162 ting fidelity that are tolerant of different protospacer-adjacent motifs, we achieved the reversion o

163 By assessing the abundance of different protospacer-adjacent motifs, we identify the Prevotella

164 rtion of the nucleotide 4 nt upstream of the protospacer adjacent motif) were increased relative to o