ライフサイエンスコーパス: pseudouridine

1 pair acceptor-TPsiC arm (where Psi indicates pseudouridine).

2 e, and can catalyze methylation at the N1 of pseudouridine.

3 bound by PKR more efficiently than mRNA with pseudouridine.

4 rupts the water-mediated interactions of the pseudouridine.

5 ible for modifying uridine13 in tRNA(Glu) to pseudouridine.

6 idine residues of rRNA by converting them to pseudouridine.

7 ted Gly-Gln dipeptide conjugated to 6'-amino-pseudouridine.

8 ns, uridines at position 39 were modified to pseudouridine.

9 cytoplasmic small subunit rRNA shown to lack pseudouridine.

10 toxification of phosphorylated compounds and pseudouridine.

11 r transcriptome-wide quantitative mapping of pseudouridine.

12 ntial for enzyme-catalyzed formation of both pseudouridines.

13 A in trans on a rescue plasmid restored both pseudouridines.

14 id produce pseudouridine 552 in 16S rRNA and pseudouridines 1199, 2605, and 2833 in 23S rRNA.

15 ferase H (RlmH) methylates 23S ribosomal RNA pseudouridine 1915 (Psi1915), which lies near the riboso

16 Of these, only pseudouridine 2605 is found naturally in either E. coli

17 ts gene product is the corresponding E. coli pseudouridine 2605 synthase.

18 Kinetic experiments confirmed that pseudouridine 2605 was the primary target.

19 ould form 23 S RNA pseudouridine 746 or tRNA pseudouridine 32 in vivo, showing that this conserved as

20 t forms 23 S rRNA pseudouridine 746 and tRNA pseudouridine 32, was deleted in strains MG1655 and BL21

21 ation of 23 S RNA pseudouridine 746 and tRNA pseudouridine 32.

22 rm either 23 S RNA pseudouridine 746 or tRNA pseudouridine 32.

23 Pseudouridine 35 (psi35) in the branch site recognition

24 zing RNAs up to 1.7 kb long as well as fully pseudouridine-, 5-methyl-C-, 2'-fluoro-, or 2'-azido-mod

25 The protein did not form pseudouridine 516 as expected but did produce pseudourid

26 rsuA, the gene for the synthase which forms pseudouridine 516 in Escherichia coli 16S rRNA, was clon

27 Pseudouridine 55 synthase (Psi55S) catalyzes isomerizati

28 of Thermotoga maritima and Escherichia coli pseudouridine 55 synthase (Psi55S) mutants in which the

29 Here we show that overexpression of the tRNA pseudouridine 55 synthase encoded by PUS4 also leads to

30 seudouridine 516 as expected but did produce pseudouridine 552 in 16S rRNA and pseudouridines 1199, 2

31 douridine synthase RluA that forms 23 S rRNA pseudouridine 746 and tRNA pseudouridine 32, was deleted

32 nsible for the in vivo formation of 23 S RNA pseudouridine 746 and tRNA pseudouridine 32.

33 ated that neither mutant could form 23 S RNA pseudouridine 746 or tRNA pseudouridine 32 in vivo, show

34 letion mutant failed to form either 23 S RNA pseudouridine 746 or tRNA pseudouridine 32.

35 For example, an A-U, inosine*U and pseudouridine*A pair each form two hydrogen bonds.

36 To determine the sequence location of pseudouridine, a combination of enzymatic hydrolysis and

37 modified nucleosides m5C, m6A, m5U, s2U, or pseudouridine ablates activity.

38 trast, in vitro transcribed mRNAs containing pseudouridine activate PKR to a lesser degree, and trans

39 elomerase activity and the cellular level of pseudouridine, an H/ACA snoRNP-mediated modification of

40 Pseudouridine, an isomer of uridine, is probably the mos

41 site significantly distorts the flipped-out pseudouridine analogue such that a change in hybridizati

42 was used to generate the naturally occurring pseudouridine analogue.

43 For nonserous tumors (n = 34 cases), pseudouridine and C36:2 phosphatidylcholine plasmalogen

44 es an enhanced, transcriptome-wide scope for pseudouridine and methods to dissect its underlying mech

45 ion of specific uridines in cellular RNAs to pseudouridines and may function as RNA chaperones.

46 Higher C-mannosyltryptophan, pseudouridine, and O-sulfo-L-tyrosine concentrations ass

47 cluding a 5' trimethylated guanosine cap, 13 pseudouridines, and 10 2'-O-methylated residues.

48 the role of PKR is validated by showing that pseudouridine- and uridine-containing RNAs were translat

49 Pseudouridines are proposed to enhance ribosome activity

50 recently identified C-mannosyltryptophan and pseudouridine as non-traditional kidney function markers

51 ndicated another major termination site: the pseudouridine at nucleotide 55.

52 ses catalyze the isomerization of uridine to pseudouridine at particular positions in certain RNA mol

53 on of the methyl group at the N3 position of pseudouridine at position 1915 causes a slight increase

54 The lack of pseudouridine at position 2504 of 23S rRNA was found to

55 Pseudouridine at position 39 (Psi(39)) of tRNA's anticod

56 e synthase 3 (Pus3), an enzyme known to form pseudouridine at positions 38 and 39 in yeast tRNA.

57 NA(Leu)), there was very slight formation of pseudouridine at that position after incubation with mPu

58 the LC/MS/MS analysis that are indicative of pseudouridine at the 5' terminus (m/z 225 --> 165), inte

59 ymethyluridine, N6-isopentenyladenosine, and pseudouridine, at positions 34, 37, and 55, respectively

60 Messenger RNAs were not known to contain pseudouridine, but artificial pseudouridylation dramatic

61 uridines at specific sites are converted to pseudouridines by H/ACA ribonucleoprotein particles (RNP

62 a, uridines in various RNAs are converted to pseudouridines by RNA-guided RNA modification complexes

63 While the biosynthesis of pseudouridine-C-nucleosides has been studied, less is kn

64 the enhanced translation of mRNAs containing pseudouridine, compared to those containing uridine, is

65 y was 0.78 for both C-mannosyltryptophan and pseudouridine concentration, and highly significant asso

66 h serum creatinine, C-mannosyltryptophan and pseudouridine concentrations showed little dependence on

67 The pseudouridine-containing hairpin is thermodynamically mo

68 e PKR to a lesser degree, and translation of pseudouridine-containing mRNAs is not repressed.

69 the characteristic dissociation reactions of pseudouridine-containing oligonucleotides following ioni

70 165, 164, 139), which permit recognition of pseudouridine-containing oligonucleotides.

71 acking interactions mediated by the U2 snRNA pseudouridines correlate with the identity of the unpair

72 s between viral RNA and tRNA(Lys3) thymidine-pseudouridine-cytidine and anticodon loops decreased the

73 Recent advances in pseudouridine detection reveal a complex pseudouridine l

74 7-nt element; (ii) loss of the 3' hairpin or pseudouridine does not affect rRNA processing; (iii) a s

75 This pseudouridine effect can also be applied to other pre-mR

76 Given that pseudouridine favors a C-3'-endo structure, our results

77 Here, the importance of pseudouridine formation (Psi) in the peptidyl transferas

78 nd five of these were verified as guides for pseudouridine formation at specific sites in ribosomal R

79 RNA pseudouridine synthase, TruB, catalyzes pseudouridine formation at U55 in tRNA.

80 of modifications remain unclear, such as for pseudouridine formation in the tRNA TPsiC arm by the bac

81 we propose a Michael addition mechanism for pseudouridine formation that is consistent with all expe

82 olar RNAs (snoRNAs), most of which guide RNA pseudouridine formation.

83 sion of stereochemistry at C2' suggests that pseudouridine generation may proceed by a mechanism invo

84 We show that AlnA and AlnB, members of the pseudouridine glycosidase and haloacid dehalogenase enzy

85 to the recognition and sequence placement of pseudouridine has not been straightforward, particularly

86 enhanced when its uridines are replaced with pseudouridines; however, the reason for this enhancement

87 This approach to pseudouridine identification is demonstrated using Esche

88 ide, single-nucleotide-resolution method for pseudouridine identification.

89 All pseudouridines identified in RNA are considered constitu

90 (RNPs) are responsible for the formation of pseudouridine in a variety of RNAs and are essential for

91 on of the transcriptome-wide distribution of pseudouridine in human and the factors governing it and

92 a class of enzymes that isomerize uridine to pseudouridine in noncoding RNAs, such as tRNA, to ensure

93 t post-transcriptionally modified nucleoside pseudouridine in nucleic acids has been developed.

94 ethod allows for the direct determination of pseudouridine in nucleic acids, can be used to identify

95 ses catalyze the isomerization of uridine to pseudouridine in RNA molecules.

96 ect the isomerization of uridine residues to pseudouridine in small nuclear RNA and ribosomal RNA.

97 n that catalyzes isomerization of uridine to pseudouridine in target RNAs.

98 We report the existence of pseudouridine in the anticodon of Escherichia coli tyros

99 Notably, the majority of pseudouridines in mRNA are regulated in response to envi

100 H/ACA RNP complexes change uridines to pseudouridines in target non-coding RNAs in eukaryotes a

101 More specifically, pseudouridines in the single-stranded loop regions of th

102 ffectively blocks the formation of important pseudouridines in U2 snRNA, as only a trace of pseudouri

103 Using pseudouridine incorporation and in vivo RNA-guided RNA p

104 nosyltryptophan and 76.0% (68.6%, 82.4%) for pseudouridine, indicating partial net reabsorption.

105 equence, one can site-specifically introduce pseudouridines into virtually any RNA (e.g., mRNA, ribos

106 The C-nucleoside pseudouridine is a natural component of RNA, and various

107 eudouridines in U2 snRNA, as only a trace of pseudouridine is detected when cells are exposed to a lo

108 Pseudouridine is found in almost all cellular ribonuclei

109 Pseudouridine is the most abundant RNA modification, yet

110 mRNA containing the nucleoside modification pseudouridine is translated longer and has an extended h

111 in pseudouridine detection reveal a complex pseudouridine landscape that includes messenger RNA and

112 Conversion of either uridine into pseudouridine leads to a splicing defect in Xenopus oocy

113 An increase in pseudouridine levels from the 10th to the 90th percentil

114 Accordingly, we found reduced pseudouridine levels in the ribosomal RNA (rRNA) of the

115 D-domain with the T-domain was enhanced by a pseudouridine located in either the D or T-domains compa

116 f the multiple characteristics attributed to pseudouridine, making messenger RNAs (mRNAs) highly tran

117 In summary, this study suggests that pseudouridine may be a novel risk factor for ovarian can

118 ition, supporting its role as an N1-specific pseudouridine methyltransferase.

119 otein required both for ribosomal RNA (rRNA) pseudouridine modification and for cellular accumulation

120 ure senescence support normal levels of rRNA pseudouridine modification and normal kinetics of rRNA p

121 ction and verification of snoRNAs that guide pseudouridine modification at more than two sites.

122 The structural effects of the pseudouridine modification at position 39 were investiga

123 des a pseudouridine synthase responsible for pseudouridine modification of 23S rRNA at three sites, a

124 erences may contribute to the ability of the pseudouridine modification to promote the bulged conform

125 Pseudouridine-modification of P6.1 slightly attenuates t

126 The three conserved pseudouridine modifications (Psi1911, Psi1915, Psi1917)

127 Our main findings are that pseudouridine modifications exhibit a range of effects o

128 e find that HCC cells lacking SNORA24-guided pseudouridine modifications have increased translational

129 With this work, 41 of the 44 known pseudouridine modifications in S.cerevisiae rRNA have be

130 f hairpin RNAs containing single or multiple pseudouridine modifications in the stem or loop regions.

131 ked whether ribosomes lacking SNORA24-guided pseudouridine modifications on 18S rRNA have alterations

132 one H/ACA snoRNA, SNORA24, which guides two pseudouridine modifications within the small ribosomal s

133 esented to allow identification of MS-silent pseudouridine modifications.

134 temperature, magnesium, and the presence of pseudouridine modifications.

135 the A+-C base-pair increases the Tm of both pseudouridine modified and unmodified RNA hairpins by 5-

136 A pseudouridine-modified region of the U2 small nuclear (s

137 ne, guanosine, uridine, inosine, xanthosine, pseudouridine, N(2)-methylguanosine, 1-methyladenosine,

138 ney function measures: C-mannosyltryptophan, pseudouridine, N-acetylalanine, erythronate, myo-inosito

139 Here, we demonstrate that N1-methyl-pseudouridine (N1mPsi) outperforms several other nucleos

140 synthesis of a 5'-O-BzH-2'- O -ACE-protected pseudouridine phosphoramidite is reported [BzH, benzhydr

141 or the two hypermodified nucleosides and for pseudouridine phosphoramidite were all greater than 98%.

142 Isomerization from uridine to pseudouridine (pseudouridylation) is largely catalyzed b

143 onylcarbamoyladenosine (ms(2)t(6)A(37)), and pseudouridine (Psi(39)) in the tRNA's anticodon domain a

144 ed the anticodon domain modified nucleosides pseudouridine (Psi(39)), 5-methylaminomethyluridine (mnm

145 s N(6)-dimethylallyl adenine (i(6)A(37)) and pseudouridine (psi(39)).

146 er, modification of the initial uridine to a pseudouridine (Psi) allows efficient recognition and rea

147 ized structure-stabilizing RNA modifications pseudouridine (Psi) and 2'-O-methylation to determine if

148 ligation-based detection and quantitation of pseudouridine (Psi) and N6-methyladenosine (m6A), two ab

149 phosphoramidite was used in combination with pseudouridine (Psi) and standard base phosphoramidites t

150 C) outperformed the current state-of-the-art pseudouridine (Psi) and/or m5C/Psi-modified mRNA platfor

151 2-thiouridine at position 34 (mcm5s2U34) and pseudouridine (psi) at position 39--two of which, ms2t6A

152 S) catalyzes isomerization of uridine (U) to pseudouridine (Psi) at position 55 in transfer RNA.

153 d the tRNAs were assayed for the presence of pseudouridine (Psi) at the expected positions.

154 ermodynamic data are reported revealing that pseudouridine (Psi) can stabilize RNA duplexes when repl

155 lements of human tRNA(Ser) are necessary for pseudouridine (Psi) formation at position 28 in the anti

156 ethylation of the first four nucleotides and pseudouridine (psi) formation at uracil 28.

157 RluB catalyses the modification of U2605 to pseudouridine (Psi) in a stem-loop at the peptidyl trans

158 r catalyzing the isomerization of uridine to pseudouridine (Psi) in ribosomal and other cellular RNAs

159 nosine (m(6)A), 5-methylcytosine (m(5)C) and pseudouridine (Psi) in RNA, and describe how these RNA m

160 ses (psi synthases) isomerize uridine (U) to pseudouridine (psi) in RNA, and they fall into five fami

161 Pseudouridine (Psi) is the most abundant internal modifi

162 Pseudouridine (Psi) is the most common chemical modifica

163 a pseudouridine synthase responsible for the pseudouridine (Psi) modifications at positions 1911, 191

164 Three pseudouridine (Psi) modifications clustered in H69 are c

165 contains an unusually dense cluster of 8-10 pseudouridine (Psi) modifications located in a three-hel

166 ite showed that a phylogenetically conserved pseudouridine (psi) residue in the segment of U2 snRNA t

167 Replacing the uridine in CCUG repeats with pseudouridine (Psi) resulted in a modest reduction of MB

168 s recessive mutations in PUS1, which encodes pseudouridine (Psi) synthase 1 (Pus1p).

169 Human pseudouridine (Psi) synthase Pus1 (hPus1) modifies speci

170 VR1]) for a chloroplast-localized homolog of pseudouridine (Psi) synthase, which isomerizes uridine t

171 l nucleolar protein Cbf5p is the most likely pseudouridine (Psi) synthase.

172 Pseudouridine (Psi) synthases catalyze the formation of

173 Pseudouridine (Psi) synthases catalyze the isomerization

174 N(1)-methyladenosine, 5-methylcytosine, and pseudouridine (Psi) via bisulfite treatment of RNA provi

175 Pseudouridine (Psi) was recently established to be wides

176 udouridine synthases isomerize (U) in RNA to pseudouridine (Psi), and the mechanism that they follow

177 odifications present in mRNA coding regions, pseudouridine (Psi), impacts protein synthesis using a f

178 seudouridylation (conversion of uridine into pseudouridine (Psi), ref. 4) of nonsense codons suppress

179 Pseudouridine (psi), the most abundant of the modified b

180 leophile for the PsiS-catalyzed formation of pseudouridine (Psi).

181 osine (m(6)A), 5-methylcytosine (m(5)C), and pseudouridine (Psi).

182 Uridines 56 and 93 are both modified to pseudouridines (Psi) during nutrient deprivation, while

183 Briefly, we mapped pseudouridines (Psi) on rRNA by Psi-seq in procyclic for

184 modifications 2-thiouridine, s(2)U(34), and pseudouridine, Psi(39), appreciably stabilized the inter

185 U2 small nuclear RNA (snRNA) contains three pseudouridines (Psi35, Psi42, and Psi44).

186 The isomerization of up to 100 uridines to pseudouridines (Psis) in eukaryotic rRNA is guided by a

187 Forty-four of the 46 pseudouridines (Psis) in the cytoplasmic rRNA of Sacchar

188 Here, we investigated the presence of pseudouridines (Psis) on the spliceosomal small nuclear

189 ns modified nucleotides, including conserved pseudouridines (Psis) that can have subtle effects on st

190 Pseudouridine represents a potential novel risk factor f

191 sensitivity, and further, more than a single pseudouridine residue is involved, as alteration of sing

192 leic acids, can be used to identify modified pseudouridine residues and can be used with general modi

193 ity are approximately additive when multiple pseudouridine residues are present.

194 Pseudouridine residues can be identified in intact nucle

195 s helix 69 of 23S rRNA, which contains three pseudouridine residues in its loop region.

196 herichia coli 23S rRNA were synthesized with pseudouridine residues located at positions 1911, 1915 a

197 n units of 252 Da) will denote the number of pseudouridine residues present.

198 three different structural contexts for the pseudouridine residues were examined and compared with t

199 Adjacent pseudouridine residues were found in the single-stranded

200 After chemical derivatization all pseudouridine residues will contain a 252 Da 'mass tag'

201 RluD catalyses formation of three pseudouridine residues within helix 69 of the 50S riboso

202 2-morpholinoethyl)carbodiimide to derivatize pseudouridine residues.

203 transcriptional modifications of RNA, except pseudouridine, result in a mass increase in the canonica

204 in vitro using modified uridine 2' fluoro or pseudouridine ribonucleotides lacked signaling activity

205 mic stability of the RNA hairpin relative to pseudouridine; RNAs containing either uridine or 3-methy

206 d by gene disruption and loss of the cognate pseudouridine site.

207 new base-pairings between snoRNAs and known pseudouridine sites in S.cerevisiae rRNA, 12 of which we

208 Comparison of the four pseudouridine sites yielded a consensus recognition sequ

209 This negative impact on splicing is pseudouridine specific, as no effect is observed when th

210 by multiple detection methods, which include pseudouridine-specific chemical derivatization and gas p

211 rRNA pseudouridine stoichiometries are conserved but reduced

212 of macrophages with a F. tularensis LVS rluD pseudouridine synthase (FTL_0699) mutant resulted in dim

213 We identified a pseudouridine synthase (PUS), mPus1p, as a coactivator f

214 Pseudouridine synthase 1 (Pus1p) is an unusual site-spec

215 The PUS1 gene encodes the enzyme pseudouridine synthase 1 (Pus1p) that is known to pseudo

216 tified a homozygous missense mutation in the pseudouridine synthase 1 gene (PUS1) in all patients wit

217 A cDNA encoding mouse pseudouridine synthase 3 (mPus3p) has been cloned.

218 redicted protein has 34% identity with yeast pseudouridine synthase 3 (Pus3), an enzyme known to form

219 Pseudouridine Synthase 4 (Pus4) and the Actin Patch Prot

220 r novel domain, designated PUA domain, after PseudoUridine synthase and Archaeosine transglycosylase,

221 rate bound to the ribonucleoprotein particle pseudouridine synthase and enzyme activity assay confirm

222 Ten methyltransferases and one pseudouridine synthase are required for complete modific

223 ific H/ACA RNA and four common proteins, the pseudouridine synthase Cbf5, Nop10, Gar1, and Nhp2.

224 stand alone pseudouridine synthases, the RNP pseudouridine synthase comprises multiple protein subuni

225 ight of the global dissimilarity between the pseudouridine synthase families, we changed the aspartic

226 ture of the RNA-modifying enzyme, psi55 tRNA pseudouridine synthase from Mycobacterium tuberculosis,

227 our findings also support the assignment of pseudouridine synthase function to certain physiological

228 tic acid residue might be a prerequisite for pseudouridine synthase function.

229 tRNA pseudouridine synthase I (PsiSI) catalyzes the conversio

230 TruD, a recently discovered novel pseudouridine synthase in Escherichia coli, is responsib

231 Analysis of total tRNA isolated from E. coli pseudouridine synthase knock-out mutants identified RluF

232 pseudouridine synthases (PUS) uncovers which pseudouridine synthase modifies each site and their targ

233 e caused by mostly missense mutations in the pseudouridine synthase NAP57 (dyskerin/Cbf5).

234 The rluC gene encodes a pseudouridine synthase responsible for pseudouridine mod

235 The Escherichia coli rluD gene encodes a pseudouridine synthase responsible for the pseudouridine

236 e Escherichia coli gene rluA, coding for the pseudouridine synthase RluA that forms 23 S rRNA pseudou

237 activates hibernating ribosomes via 23S rRNA pseudouridine synthase RluD, which increases ribosome ac

238 Pseudouridine synthase RluE modifies U2457 in a stem of

239 Escherichia coli pseudouridine synthase RluF is dedicated to modifying U2

240 The DKC1 gene encodes a pseudouridine synthase that modifies ribosomal RNA (rRNA

241 Mutations in DKC1, encoding for dyskerin, a pseudouridine synthase that modifies rRNA and regulates

242 ar ribonucleoprotein complexes and acts as a pseudouridine synthase to modify newly synthesized ribos

243 Such a role for cysteine in the pseudouridine synthase TruA (also named Psi synthase I)

244 icodon stem loop (ASL) by a highly conserved pseudouridine synthase TruA.

245 The pseudouridine synthase TruB handles 5-fluorouridine in R

246 we prove the tRNA chaperone activity of the pseudouridine synthase TruB, reveal its molecular mechan

247 in the tRNA TPsiC arm by the bacterial tRNA pseudouridine synthase TruB.

248 he downstream genes ppnK (NAD kinase), rluE (pseudouridine synthase), and pta (phosphotransacetylase)

249 guide RNA and four essential proteins: Cbf5 (pseudouridine synthase), L7Ae, Gar1 and Nop10 in archaea

250 Dyskerin is a putative pseudouridine synthase, and it has been suggested that D

251 Nine methyltransferases, as well as the pseudouridine synthase, are already known.

252 The pseudouridine synthase, Cbf5, is also the protein that s

253 A guide RNA and four proteins, including the pseudouridine synthase, Cbf5.

254 e, TERC, and other components, including the pseudouridine synthase, dyskerin, the product of the DKC

255 idine, bound to a ribonucleoprotein particle pseudouridine synthase, strongly prefer the syn glycosid

256 RNA pseudouridine synthase, TruB, catalyzes pseudouridine fo

257 luciferase reporter and identified the tRNA pseudouridine synthase, TruB1.

258 morphic alleles of nop60B, a gene encoding a pseudouridine synthase.

259 4, an uncharacterized mitochondrial putative pseudouridine synthase.

260 id residue is catalytically essential in one pseudouridine synthase.

261 antly alter the catalytic activity of either pseudouridine synthase.

262 f the TruA, TruB, RsuA, and RluA families of pseudouridine synthases (PsiS) identifies a strictly con

263 tructural comparisons with other families of pseudouridine synthases (PsiS) indicate that Psi55S may

264 Perturbing pseudouridine synthases (PUS) uncovers which pseudouridi

265 ery similar to the catalytic domain of other pseudouridine synthases and a second large domain (149 a

266 tural properties that are unique among known pseudouridine synthases and are consistent with its dist

267 roline residues in Motif I of RluA and TruB, pseudouridine synthases belonging to different families.

268 The pseudouridine synthases catalyze the isomerization of ur

269 The pseudouridine synthases catalyze the isomerization of ur

270 ue is critical for the catalytic activity of pseudouridine synthases from two additional families of

271 de a resource for identifying the targets of pseudouridine synthases implicated in human disease.

272 s little sequence homology with the other 10 pseudouridine synthases in E. coli which themselves have

273 The pseudouridine synthases isomerize (U) in RNA to pseudour

274 e alignments using the first four identified pseudouridine synthases led Koonin and, independently, S

275 ry to probe the role of cysteine residues in pseudouridine synthases of the families that do not incl

276 ence and structural comparisons suggest that pseudouridine synthases of the RluA, RsuA, and TruA fami

277 The predicted SwoCp is homologous to rRNA pseudouridine synthases of yeast (Cbf5p) and humans (Dkc

278 n that there are four distinct "families" of pseudouridine synthases that share no statistically sign

279 On the basis of sequence alignments, the pseudouridine synthases were grouped into four families

280 in the ribosomal protein S4, two families of pseudouridine synthases, a novel family of predicted RNA

281 ase, was detected in archaeal and eukaryotic pseudouridine synthases, archaeal archaeosine synthases,

282 modification sites to one of seven conserved pseudouridine synthases, Pus1-4, 6, 7 and 9.

283 an active site cleft, conserved in all other pseudouridine synthases, that contains invariant Asp and

284 Different from stand alone pseudouridine synthases, the RNP pseudouridine synthase

285 to be learned about the RNA targets of human pseudouridine synthases, their basis for recognizing dis

286 ficant relative to turnover by the wild-type pseudouridine synthases.

287 ends on both site-specific and snoRNA-guided pseudouridine synthases.

288 uridine (or pseudouridylation) catalyzed by pseudouridine synthases.

289 However, neither rRNA pseudouridine synthesis nor rRNA processing appears to b

290 RNA methylases, a yeast protein containing a pseudouridine synthetase and a deaminase domain, bacteri

291 ssembly factors, such as helicases, GTPases, pseudouridine synthetases, and methyltransferases, are a

292 Pseudouridine, the most abundant modified nucleoside in

293 the C-C (rather than C-N) glycosidic bond of pseudouridine, the otherwise common dissociation paths i

294 52 Da 'mass tag' that allows the presence of pseudouridine to be identified using mass spectrometry.

295 are used to narrow the sequence location of pseudouridine to specific T1 fragments in the gene seque

296 the top half domain composed of acceptor and pseudouridine (TPsiC) arms is more ancient than the bott

297 Pseudouridine was verified by multiple detection methods

298 The respective concentrations for pseudouridine were 2.89/5.67 micromol/L and 39.7/33.9 mi

299 The known molecular functions of pseudouridine, which include stabilizing RNA conformatio

300 sine bulge is associated with a well-stacked pseudouridine, which is linked via an ordered water mole