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1 vels in a Dicer null cell line, and (3) Ago2 pulldown.
2 ined four proteins on an anti-FABP1 antibody pulldown.
3 CSNAP (Chemical Similarity Network Analysis Pulldown), a new computational target identification met
4 Here we miniaturize the concept of affinity pulldown, a gold-standard in vitro PPI interrogation tec
9 ransfer (FRET) and glutathione S-transferase pulldown analyses identified Akt1 pleckstrin homology do
11 e EGFR by coimmunoprecipitation and affinity pulldown analyses, and the primarily trans-Golgi network
12 Here, using mutagenesis-based kinetic and pulldown analyses, we show that Noonan syndrome Ras muta
18 e globular regions of C1q as demonstrated by pulldown and cell surface co-localization experiments.
20 The interaction was confirmed by in vitro pulldown and co-immunoprecipitation assays and was shown
24 P1 interaction was confirmed by both peptide pulldown and coimmunoprecipitation assays, which also de
28 is an interacting partner of IRSp53 through pulldown and Forster resonance energy transfer analysis,
30 o glutathione S-transferase (GST) E-cadherin pulldown and immunoblot analysis to assess levels of unc
31 man cell lines, fluorescence microscopy, and pulldown and immunoblotting assays, we show that alpha-d
36 olecular mechanisms were investigated by RNA pulldown and immunoprecipitation, mass spectrometry, mic
39 bination of GFP nanotrap association assays, pulldown and integrin-binding assays, and live-cell imag
45 , coimmunoprecipitation and HDAC assays, and pulldown and NMR experiments, we show that HDAC1/2 deace
46 d PAT1 was further confirmed by in vitro GST pulldown and overlay assays and in intact neutrophils an
48 Vav3-Cdc37 interaction was confirmed by GST pulldown and, for native proteins, by co-immunoprecipita
54 MORF proteins by yeast two-hybrid, in vitro pulldown, and bimolecular fluorescence complementation a
55 Ai, in situ epitope tagging of proteins, GST pulldown, and coimmunoprecipitation assays, and immunofl
58 tography, Forster resonance energy transfer, pulldown, and in vitro GEF assays to demonstrate that re
59 in staining, delayed competition in affinity pulldown, and inhibition of TNF biogenesis after washout
60 he inclusion of any estimate of SssI Control pulldown, and is comparable to - and in some cases bette
61 human and murine cells; immunoprecipitation, pulldown, and surface plasmon resonance assays; and immu
63 g, peptide-N-glycosidase F treatment, lectin pulldowns, and exoglycosidase treatment, we have now inv
64 ed Hspb1 mRNA in wild-type lens lysate TDRD7-pulldowns, and single-molecule RNA imaging showed co-loc
65 onuclease-deficient Cas9-based gene promoter pulldown approach coupled with mass spectrometry, we fou
70 Similarly, results from CBX4-BioTAP protein pulldowns are consistent with reports of a diversity of
71 rabidopsis and Nicotiana benthamiana using a pulldown assay and fluorescence resonance energy transfe
73 interaction by site-directed mutagenesis and pulldown assay and thereby confirm that the major bindin
74 s work establishes the single-molecule lipid pulldown assay as a simple and highly sensitive approach
78 to embryonic organ explants, with a microRNA pulldown assay that allows direct identification of micr
80 phila melanogaster and performed an unbiased pulldown assay to identify all possible interactions, re
82 V envelope glycoproteins were also used in a pulldown assay with beads coated with heparin, a close H
83 s and analyzed Rab5 binding with an in vitro pulldown assay with GST-Rab5(GTP) Of the 35 p110beta hel
84 otein-RNA reconstitution and a stringent RNA pulldown assay with human choriocarcinoma (JAR) cells, w
85 ctivity enzyme-linked immunosorbent assay, a pulldown assay, and immunostaining with a monoclonal ant
86 ipt in human T cells and found, using biotin pulldown assay, that HuR specifically interacts with its
98 70-binding site in SOD2, we used a series of pulldown assays and showed that hsp70 binds to the amino
99 DM2 required for p21(Waf1) degradation using pulldown assays and Western blotting and then examined t
101 ation analysis, immunoprecipitation, and GST pulldown assays based on the theoretical predictions rev
102 We used L-selectin cytoplasmic tail peptide pulldown assays combined with high sensitivity liquid ch
111 erminal kinase domain combined with in vitro pulldown assays demonstrated that eriodictyol, a flavano
117 roteins within platelets and confirmation by pulldown assays followed by immunoblotting, we identifie
119 trometry and glutathione S-transferase (GST) pulldown assays identified integrin alpha5 as a novel Sc
121 , isothermal titration calorimetry data, and pulldown assays indicated that CaM-N and CaM-C both can
123 In this study, glutathione S-transferase pulldown assays indicated that residues 1 to 68 of UL84
133 pitation and glutathione S-transferase (GST) pulldown assays revealed that GBP1 interacted with the N
134 ctivity in RIalpha-knockdown cells, and cAMP pulldown assays revealed that P-REX1 preferentially inte
140 by absorbance spectroscopy and hemin-agarose pulldown assays showed that Shu1 interacts with hemin, w
145 selected biochemical pathways; (c) affinity pulldown assays that, in some cases, confirm and even ex
146 al mRNA-binding proteins identified from RNA pulldown assays to determine which of these exhibit bona
147 Here, we used biolayer interferometry and pulldown assays to identify regions of RAG1 necessary fo
149 depending on its CTD phosphorylation state, pulldown assays were performed using the CTD of the duck
150 lectrophoretic mobility shift assays and DNA pulldown assays with ChIP-PCR confirmed that MZF1 binds
155 say that combines principles of conventional pulldown assays with single-molecule fluorescence micros
156 ults from flow cytometry, live-cell imaging, pulldown assays, and genetically-modified cell lines sup
157 Here, using size-exclusion chromatography, pulldown assays, and small angle x-ray scattering, we sh
158 brid mating and co-transformation protocols, pulldown assays, and surface plasmon resonance analysis.
160 biophysical methods, including heterocomplex pulldown assays, far-UV CD spectroscopy, the thioflavin
161 ombination of kindlin knockdown, biochemical pulldown assays, fluorescence microscopy, fluorescence r
162 oplasmic capping complex was demonstrated by pulldown assays, gel filtration and proximity-dependent
167 acted with the helicase domain of BKV Tag in pulldown assays, suggesting that NFI helps recruit Tag t
171 g luciferase p-miR-Report constructs and RNA pulldown assays, we confirmed that miR-511 bound directl
175 ce energy transfer experiments, and in vitro pulldown assays, we have now identified the key residues
176 Using the human Cad11 cytoplasmic domain in pulldown assays, we identified human angiomotin (Amot),
177 pitation (co-IP), mass spectrometry, and GST pulldown assays, we identified poly(ADP-ribose) polymera
178 re, using an array of immunofluorescence and pulldown assays, we report that expression of active or
179 assays, and glutathione S-transferase (GST) pulldown assays, we show that NR2A subunits interact dir
197 ng endosome compartments were seen following pulldown by immunoaffinity chromatography with Rab-speci
198 n was confirmed by glutathione S-transferase pulldown, coimmunoprecipitation, and laser confocal micr
199 s (colocalization, glutathione S-transferase pulldown, coimmunoprecipitation, yeast two-hybrid, gel s
200 this stem-loop region using an RNA affinity pulldown-coupled mass spectrometry approach and identifi
201 in this study, BayMeth used with our modeled pulldown coverage performs better than BayMeth run witho
204 T84D enhances the ability of origin ssDNA to pulldown Dpb11, and Sld2 binding to origin ssDNA may be
205 reated samples, from which we also find that pulldown efficiency sharply increases for DNA fragments
207 equence specificity of MBD2-DNA binding in a pulldown experiment revealing three potential biases in
209 yl-CpG/MBD2 binding in the context of an MBD pulldown experiment, we build a model of expected MBD pu
210 third protein in yeast three-hybrid assays, pulldown experiments (luminescence-based mammalian inter
214 However, the target was identified based on pulldown experiments and in vitro binding data, without
217 Yeast two-hybrid, co-immunoprecipitation and pulldown experiments demonstrate Piasy and Pias1 interac
223 ct interaction between Med19 and Med1 by GST pulldown experiments indicating privileged contacts betw
230 and glutathione S-transferase fusion protein pulldown experiments show that tyrosol-phosphorylated Er
234 ological measurements in Xenopus oocytes and pulldown experiments to analyze the direct interaction b
238 affinity for matrix compared with p6 in GST-pulldown experiments was higher for ALIX than for TSG101
239 unoprecipitation and AQP0 C-terminal peptide pulldown experiments were used to confirm the protein-pr
240 ial ligand lipopolysaccharide and subsequent pulldown experiments with biotin-avidin affinity chromat
243 eins interacting with GlyRbeta, we performed pulldown experiments with rat brain extracts using a glu
244 wo proteins using co-immunoprecipitation and pulldown experiments with truncated or mutant Drosophila
245 trast, results from kinetic studies, heparin pulldown experiments, and inhibition experiments with an
246 analysis, immunoprecipitations, mutagenesis, pulldown experiments, and peptide arrays constrained PP1
248 ected mutagenesis, glutathione S-transferase pulldown experiments, immunofluorescence, molecular mode
250 ts in HEK-293 cells and Xenopus oocytes with pulldown experiments, we analyzed the direct interaction
254 to monitor Myo1c phosphorylation, along with pulldown, fluorescence binding, and additional biochemic
259 This is confirmed by comparing input versus pulldown high-throughput sequencing data on M.SssI-treat
260 o anti-ubiquitin were seen in the optineurin pulldown, indicating that optineurin was ubiquitinated.
261 shion, SDCP-MS (SNP-specific DNA competition pulldown-mass spectrometry) to identify fSNP-bound prote
262 pled to a green fluorescent protein-nanotrap pulldown methodology and liquid chromatography-tandem ma
263 Cq-IGFBP) protein was produced and, using a "pulldown" methodology, was shown to specifically interac
266 re, we used immunoprecipitation and affinity pulldown of ectopically expressed p30 coupled with mass
267 ircPVT1, only let-7 was found enriched after pulldown of endogenous CircPVT1, suggesting that CircPVT
268 ic peptides, as well as CaM-agarose affinity pulldown of full-length recombinant BdALMT12, we confirm
269 ts an initial slow slip followed by a sudden pulldown of the Philippine Sea slab so rapid that it cau
273 ed these findings through deep sequencing of pulldown regions and whole-genome sequencing of independ
274 biological approaches, including active RAS pulldown, reporter and Comet assays, small interfering R
281 munoprecipitation and biotin-labeled miR-665 pulldown studies identified Kat6a as another potential t
288 ed animals demonstrated stronger acute viral pulldown than controls, but a trend for higher acute vir
289 , we have utilized glutathione S-transferase pulldowns, two-hybrid analysis, and NMR to demonstrate t
293 lly induced dimerization and single-molecule pulldown, we revealed that increasing the proximity of a
295 By using glutathione S-transferase (GST) pulldowns, we identified an essential role of lysine 343
296 density gradient centrifugation and antibody pulldowns, we show that all six A subunits are associate
299 proteins and AIDP-Wb (allele-imbalanced DNA pulldown-Western blot) to detect allele-specific protein
300 ots, reporter assays, biotin-tagged promoter pulldown with proteomics, and loss-of-function studies.