1 s were determined using (35) S radiolabeling
pulse chase.
2 trial elemental cycling via a stable isotope
pulse-chase.
3 rasynaptic components would be compared with
pulse-chase (
15)N labeling in mice and (14)C content in
4 However,
pulse-chase 5-bromodeoxyuridine-labeling assay revealed
5 Herein, we develop ratiometric
pulse-chase amidination mass spectrometry (rPAm-MS) as a
6 In the latter samples,
pulse-chase analyses demonstrated the disappearance of e
7 e determine translation rates of messages by
pulse-chase analyses in living Escherichia coli cells an
8 Pulse-chase analyses reveal that active site inhibitors
9 Moreover,
pulse-chase analyses reveal that S-palmitoylation is imp
10 Pulse-chase analyses revealed that a portion of Tpk2 kin
11 Pulse-chase analyses revealed that residues 9-20 of the
12 Immunoblot and
pulse-chase analyses revealed that the glycosylation-def
13 Localization and metabolic
pulse-chase analyses with site-directed mutants and chim
14 Metabolic
pulse-chase analyses, small molecule inhibitor treatment
15 Using lineage tracing and DNA
pulse-chase analyses, we identified an anatomical locati
16 Using a combination of steady state and
pulse-chase analyses, we show that FKBP38 knockdown incr
17 Furthermore, the primer-extension
pulse-chase analysis affirmed that the reconstituted N.
18 However, ex vivo
pulse-chase analysis clearly shows that in the absence o
19 Pulse-chase analysis demonstrated that the absence of th
20 Pulse-chase analysis demonstrates delayed kinetics of 35
21 In agreement, bromodeoxyuridine (BrdU)
pulse-chase analysis demonstrates that the absence of AS
22 Pulse-chase analysis in HEK293F cells indicated that the
23 Pulse-chase analysis indicated that in S6-expressing cel
24 Pulse-chase analysis indicated that the steady-state tur
25 Pulse-chase analysis indicates that these Hsp70/Hsp110 t
26 Fluorescence
pulse-chase analysis of an internally tagged Cse4 reveal
27 HLA-B27 assembly was assessed by
pulse-chase analysis of B27 molecules, and UPR triggerin
28 erived macrophages (MDM) and microglia using
pulse-chase analysis of fibrillar and oligomer (125)I-Ab
29 Using metabolic labeling and
pulse-chase analysis of HIV-1 Gag proteins, we verified
30 with orthogonal clickable fluorophores, dual
pulse-chase analysis of Lck revealed accelerated palmita
31 Pulse-chase analysis of p21 protein stability revealed t
32 Pulse-chase analysis of PrP and APP by fluorescent gel i
33 Pulse-chase analysis revealed that all six nsp2 species
34 Pulse-chase analysis revealed that N470D had a prolonged
35 Pulse-chase analysis revealed that PP4 decreased the hal
36 Pulse-chase analysis revealed that pre-termination ribos
37 Pulse-chase analysis revealed that the initial particle
38 In addition,
pulse-chase analysis revealed that the proteasome-depend
39 ne was also predominantly extrasynaptic, and
pulse-chase analysis revealed that these newly inserted
40 ion augmented surface CTLA-4 expression, and
pulse-chase analysis showed a more rapid transport of CT
41 Pulse-chase analysis showed that the rates and amount of
42 Pulse-chase analysis showed that Triton-insoluble, newly
43 In parallel, we used
pulse-chase analysis to measure the transport of two sec
44 temperature to slow the assembly process and
pulse-chase analysis with immunodetection methods, we fo
45 Quantitative imaging,
pulse-chase analysis, and high-resolution ratiometric li
46 yzed by Western blotting, Northern blotting,
pulse-chase analysis, and immunofluorescence to assess t
47 Parabiosis, bromodoxyuridine (BrdU)
pulse-chase analysis, and intranasal instillation of tra
48 As shown through a
pulse-chase analysis, the mdVp1s were derived from the n
49 By
pulse-chase analysis, the prp43 mutant is defective in t
50 Using
pulse-chase analysis, we characterized SMN protein turno
51 Using microscopy-based
pulse-chase analysis, we find that Smo moves through a l
52 Moreover, using
pulse-chase analysis, we show that this activation is ma
53 Pulse chase and arrest of autophagy at the pre-proteolys
54 Pulse chase and mutational analysis indicated that HRD1
55 eased the transcription of Bach1 as shown by
pulse chase and real time PCR experiments.
56 As measured by
pulse-chase and cycloheximide chase assays, a major bind
57 Pulse-chase and cycloheximide-chase assays demonstrated
58 Pulse-chase and endoglycosidase H analysis demonstrate t
59 Pulse-chase and kinetic spectral experiments and modelin
60 Employing histone and nucleotide double-
pulse-chase and lineage tracing, we show that the early
61 We have developed
pulse-chase and mating assays to follow the fate of exis
62 ng and sequencing (Bru-Seq) and bromouridine
pulse-chase and sequencing (BruChase-Seq) to assess geno
63 king to the cell surface tested in metabolic
pulse-chase and surface biotinylation assays, respective
64 rotein processing and stability by metabolic
pulse-chase and surface On-Cell Western blots revealed t
65 We used EdU
pulse-chase and tissue-reconstruction approaches to anal
66 We used stable-isotope
pulse-chase and total proteome mass-spectrometry to iden
67 Pulse-chase application of azidohomoalanine and homoprop
68 We show that the HaloTag
pulse-chase approach is a nontoxic alternative to inhibi
69 Here we show, by combining two different
pulse-chase approaches--genetic fate-mapping with stable
70 A
pulse-chase assay of common SDHB missense mutations in t
71 Moreover, a
pulse-chase assay showed that two chimeric precursors wi
72 We report the development of a
pulse-chase assay to monitor function recovery after che
73 ntaining radioactive auxin to be removed for
pulse chase assays that determine transport rates.
74 on-N-glycosylated F27C variants in metabolic
pulse-chase assays coupled with flow cytometry and cell
75 (3)H-uridine
pulse-chase assays demonstrate that BLM expression is re
76 rrays of immobilized 11-residue peptides and
pulse-chase assays to examine the substrate specificity
77 Pulse-chase assays with BrdU confirmed intracellular rep
78 2)H formation and a lack of equilibration in
pulse-chase assays) were also seen with (rat) P450 2B1,
79 Data from Western immunoblot assays,
pulse-chase assays, and immunoprecipitation assays show
80 Using [(35)S]methionine
pulse-chase assays, we observed that the Isc pathway, bu
81 vealed through a combination of the BrdU/EdU
pulse-chase,
based on the circadian pattern of DNA repli
82 cocaine analog JHC1-64 and by reversible and
pulse-chase biotinylation assays showing evidence for ly
83 Indeed,
pulse-chase biotinylation experiments in IECs lacking An
84 Prox-1 immunohistochemistry and
pulse-chase bromodeoxyuridine labeling showed that proge
85 Pulse-chases combined with mutagenesis studies reveal th
86 neuroendocrine cell line lacking PC1, under
pulse-chase conditions release is constitutive and compo
87 Pulse-chase dye labeling experiments revealed that the e
88 owever, in vitro binding studies utilizing a
pulse-chase electrophoretic mobility shift assay protoco
89 ctions using steady-state, pre-steady-state,
pulse-chase,
equilibrium-binding titrations, and stopped
90 tail loss, we performed a bromodeoxyuridine
pulse-chase experiment and found that a subset of ependy
91 Examination of selected mutants during a
pulse-chase experiment demonstrated an increase in F pro
92 ks the starting point for a crystallographic
pulse-chase experiment of the active site during turnove
93 A
pulse-chase experiment on living cells showed that the r
94 Interestingly, both Western blotting and a
pulse-chase experiment showed co-immunoprecipitation of
95 We employed a carbon-13
pulse-chase experiment to investigate how a temperate es
96 A
pulse-chase experiment was used to demonstrate that even
97 Here, using a (13)C
pulse-chase experiment, we demonstrate how trophic struc
98 cells when compared with control cells in a
pulse-chase experiment.
99 Pulse chase experiments in 293 T cells expressing rhGAA
100 Pulse chase experiments indicate that the newly-generate
101 In
pulse chase experiments using a deconvolved, confocal li
102 Using nucleoside
pulse-chase experiments and clonal analysis, we determin
103 EdU
pulse-chase experiments and in vivo tracking of individu
104 Here we performed
pulse-chase experiments and showed that the C29/C30 ster
105 The study shows that (13)CO2
pulse-chase experiments are powerful in elucidating, und
106 Lipid turnover rates were studied by
pulse-chase experiments at the single cell level.
107 Isotope
pulse-chase experiments confirm that all intermediates o
108 Pulse-chase experiments confirm that LRAD3 expression si
109 (14)CO2
pulse-chase experiments confirmed that water deficit enh
110 Pulse-chase experiments could not detect newly assembled
111 rrest of tumour cell proliferation with TMZ,
pulse-chase experiments demonstrate a tumour re-growth c
112 Last,
pulse-chase experiments demonstrate that Hsp70 preferent
113 Pulse-chase experiments demonstrate that in cells expres
114 Pulse-chase experiments demonstrate that the bulk popula
115 EdU
pulse-chase experiments demonstrated a perivascular canc
116 Western immunoblots and
pulse-chase experiments demonstrated that EsaR is stable
117 Pulse-chase experiments demonstrated that in the presenc
118 Pulse-chase experiments demonstrated that NGF treatment
119 Pulse-chase experiments demonstrated that SpoIVFA synthe
120 Pulse-chase experiments demonstrated that the down-regul
121 BrdU
pulse-chase experiments demonstrated the longevity of th
122 biosynthesis of both proteins were similar,
pulse-chase experiments established that the apparent ha
123 The 5-ethynyl-2'-deoxyuridine
pulse-chase experiments further reveal that this stem ce
124 In organello, pulse labelling and
pulse-chase experiments have enabled us to track the mit
125 Pulse-chase experiments identified sites of cell surface
126 Pulse-chase experiments in COS-7 cells show that there i
127 Pulse-chase experiments in gonococci demonstrated that L
128 f the elemental effect using dNTPalphaS, and
pulse-chase experiments indicate that a rapid phosphodie
129 Pulse-chase experiments indicated decreased protein turn
130 Optical
pulse-chase experiments of Dendra2-tau demonstrate that
131 They enable regional optical marking in
pulse-chase experiments on live cells and tissues, and t
132 Pulse-chase experiments reveal a delay in rRNA processin
133 First, BrdU
pulse-chase experiments reveal that CD44(+) cells coloca
134 Immunofluorescence microscopy and
pulse-chase experiments reveal that stabilization of ORF
135 nding occurred at 2.1 muM(-1) s(-1), and the
pulse-chase experiments revealed an ATP-promoted isomeri
136 sis of polyadenylated RNA stability via 5-EU
pulse-chase experiments revealed RNAs with shorter half-
137 Pulse-chase experiments revealed that A774wt and avirule
138 Pulse-chase experiments revealed that IE2-86 but not IE1
139 Pulse-chase experiments revealed that newly made protein
140 nously expressing PC1, both steady-state and
pulse-chase experiments revealed that peptides derived f
141 Fate mapping using BrdU
pulse-chase experiments revealed that such deficits may
142 Furthermore,
pulse-chase experiments revealed that the binding of QPD
143 A series of
pulse-chase experiments revealed that the origin of aort
144 HO cells stably expressing the hKOR-N25/39Q,
pulse-chase experiments revealed that the turnover rate
145 Pulse-chase experiments revealed that the turnover rate
146 Rather,
pulse-chase experiments show that decreases in steady st
147 Metabolic
pulse-chase experiments show that TC10 did not affect CF
148 Pulse-chase experiments showed an enhanced degradation a
149 affinity of the aldehydes for P450 2A6, but
pulse-chase experiments showed only limited exchange wit
150 Pulse-chase experiments showed that a fraction of gO rem
151 Pulse-chase experiments showed that collagen type I secr
152 Significantly,
pulse-chase experiments showed that cotransfection of Nr
153 Pulse-chase experiments showed that PreGN C-terminal cle
154 Pulse-chase experiments showed that the interaction betw
155 Long-term
pulse-chase experiments showed that the mature DeltaRI/D
156 Pulse-chase experiments showed that UT-A1 half-life is r
157 ckdown, REGgamma-deficient MEF analysis, and
pulse-chase experiments substantiate that REGgamma promo
158 Here, we report genetic
pulse-chase experiments that define osteoblastic cells a
159 To overcome this difficulty, we used Mg(2+)
pulse-chase experiments to differentiate each reaction i
160 echniques to follow formation of NR by using
pulse-chase experiments to examine protein and lipid del
161 In
pulse-chase experiments to examine the effects of Ad-Acs
162 r organ culture and bromodeoxyuridine (BrdU)
pulse-chase experiments to identify and evaluate stem ce
163 By combining
pulse-chase experiments to measure the rate at which the
164 Finally,
pulse-chase experiments using [(14)C]serine revealed tha
165 nucleocapsids into virions as determined in
pulse-chase experiments was dependent on the activity of
166 action time courses, substrate trapping, and
pulse-chase experiments were used to assess folate relea
167 Pulse-chase experiments with (14)CO(2) show that transpo
168 Pulse-chase experiments with 5-EU allowed us to determin
169 inetic lags were observed in acid formation;
pulse-chase experiments with carrier aldehydes showed on
170 Optical
pulse-chase experiments with Dendra2-tagged aSyn version
171 Through
pulse-chase experiments with halogenated thymidine analo
172 Pulse-chase experiments with radiolabeled sugars and ami
173 Bromodeoxyuridine
pulse-chase experiments with short survival times sugges
174 In
pulse-chase experiments, 14-3-3beta increased the synthe
175 In fact, as directly demonstrated by
pulse-chase experiments, EECs in the vascularized, but n
176 In
pulse-chase experiments, either miR-33 overexpression or
177 In
pulse-chase experiments, recycled peptidoglycan was not
178 In
pulse-chase experiments, the primary Vhs translation pro
179 ng, molecular imaging, and [(35)S]methionine
pulse-chase experiments, together with lysosome (chloroq
180 In
pulse-chase experiments, various chase periods decreased
181 Using
pulse-chase experiments, we also discovered that the pec
182 lizing in vivo imaging and bromodeoxyuridine
pulse-chase experiments, we have analyzed growth and reg
183 es to different parts of the molecule and in
pulse-chase experiments, we showed that the C terminus o
184 sults are further supported by proteome-wide
pulse-chase experiments, which show that the loss of UCH
185 cinal plant Pentalinon andrieuxii by (13)CO2
pulse-chase experiments.
186 ociation of the intermediates as revealed by
pulse-chase experiments.
187 life ~7-10 h), which we confirmed by in vivo
pulse-chase experiments.
188 r Abeta levels and hasten its degradation in
pulse-chase experiments.
189 ar behavior of these mutants was assessed in
pulse-chase experiments.
190 reaction amplitudes between pulse-quench and
pulse-chase experiments.
191 e observed processivity in pregnenolone/DHEA
pulse-chase experiments.
192 immunohistochemistry, and bromodeoxyuridine
pulse-chase experiments.
193 hemical approach, human islets were used for
pulse-chase experiments.
194 track the fate of adult cardiomyocytes in a '
pulse-chase'
fashion: after a 4-OH-tamoxifen pulse, gree
195 Fluorescent
pulse-chase fate-tracking documented dynamic nucleo-cyto
196 Pulse-chase fluorescent labeling indicates that Nup188 p
197 We use a new in cellula
pulse-chase imaging protocol with photoactivatable GFP t
198 Pulse-chase,
immunofluorescence, and electron microscopy
199 Pulse-chase immunoprecipitation experiments indicated th
200 Pulse-chase,
immunoprecipitation/immunoblotting analyses
201 Finally, a
pulse-chase isotope enrichment experiment was conducted
202 Pulse-chase kinetic transport assays on four of the top-
203 Acid-quench and
pulse-chase kinetics indicated that an isomerization ste
204 eins bind and initiate assembly prior to the
pulse-chase kinetics.
205 0A on wild-type activity and single-turnover
pulse-chase kinetics.
206 traperitoneally delivered every other day to
pulse-chase label in vivo endogenous cardiac replication
207 Using
pulse-chase label retention experiments and multiplexed
208 ing complementary methods of genetic H2B-GFP
pulse-chase labeling and BrdU incorporation in mice, we
209 Pulse-chase labeling and cell surface biotinylation expe
210 otein turnover in adipocytes using metabolic
pulse-chase labeling and high resolution mass spectromet
211 We addressed this question using metabolic
pulse-chase labeling and quantitative mass spectrometry
212 A new covalent fluorescent
pulse-chase labeling approach using SNAP tagging has now
213 Pulse-chase labeling demonstrated that the LHDeltaT dime
214 In the present study we use
pulse-chase labeling experiments in conjunction with iso
215 lar-weight haloalkanes as blocking agents in
pulse-chase labeling experiments with the cell-permeable
216 n, in vivo lineage tracing, and HSC-specific
pulse-chase labeling have provided novel insights on B1a
217 In vivo
pulse-chase labeling identified galactoglycerolipid pool
218 Pulse-chase labeling indicates that growth is heterogene
219 Improved
pulse-chase labeling of endogenous interphase chromosome
220 Pulse-chase labeling of mitochondrial translation produc
221 Using in vivo
pulse-chase labeling of neutrophil DNA with 6,6-(2)H2-gl
222 In addition,
pulse-chase labeling of Notch in living tissues indicate
223 Here, we describe a metabolic
pulse-chase labeling protocol using 4-thiouracil combine
224 Finally,
pulse-chase labeling reveals that ataxin-3 is degraded b
225 Pulse-chase labeling showed that TRP32 is a stable prote
226 proteasomal degradation of NHK in metabolic
pulse-chase labeling studies.
227 Here, we present a genetic screen coupled to
pulse-chase labeling that allow us to identify proteins
228 4) C]-acetate incorporation into MGDG during
pulse-chase labeling, indicating a reduced flux through
229 nt AMPA receptor subunits by using two-color
pulse-chase labeling.
230 epithelial Caco-2 cells using techniques of
pulse-chase labelling, domain-specific biotinylation and
231 he SNAP-tag for studying protein turnover by
pulse-chase labelling.
232 By combining
pulse-chase lineage tracing and single-cell RNA sequenci
233 Quantitative
pulse-chase live imaging experiments showed that overexp
234 Pulse-chase metabolic labeling reveals that the half-lif
235 Pulse-chase metabolic labeling studies demonstrated that
236 scopy, cell-surface cross-linking, FRET, and
pulse-chase metabolic labeling, we demonstrate that dele
237 biosis [9-11] using a reciprocal metabolomic
pulse-chase method.
238 conflict greatly, with a study employing C14
pulse-chase methodology concluding 1% annual turnover in
239 with amino acids in cell culture (SILAC) and
pulse-chase methods to generate a global quantitative ma
240 We used fluorescence-based
pulse-chase methods to visualize the fate of pre-existin
241 in cells have been carried out with pulse or
pulse-chase methods using radioactive isotopes.
242 Using a novel system for
pulse-chase microscopy, we have visualized the postsynth
243 ng assumptions and restrictive models in the
pulse-chase model artificially eliminated high-turnover
244 Similarly, the
pulse-chase model was acutely sensitive to assumptions o
245 Previously, a
pulse-chase monitored by quantitative mass spectrometry
246 Here we have developed a method involving
pulse-chase monitored by quantitative mass spectrometry
247 in the hippocampal dentate gyrus, using dual
pulse-chase,
multicolour gamma-retroviral tracing, trans
248 cells, we employed an inducible transgenic "
pulse-chase"
murine model (K5Tta x TRE-H2BGFP) to locali
249 Pulse-chase,
Northern blotting, and primer extension ana
250 Using a combination of lineage tracing and
pulse-chase of actively proliferating chondrocytes, we h
251 surement of protein of interest half-life by
pulse-chase of HaloTag ligands is not widely employed be
252 Pulse-chase photoconversion experiments show that molecu
253 orally separated glucose populations using a
pulse-chase protocol.
254 A BrdU
pulse-chasing protocol was also introduced as an additio
255 incorporation into replicating viral DNA and
pulse-chase protocols, we found that viral DNA synthesis
256 r actin, claudin strands break and reanneal;
pulse-chase-
pulse analysis using SNAP-tagged claudins sh
257 Pulse-chase-
pulse experiments with BrdU and EdU, and DNA
258 using a combination of RNA footprinting and
pulse-chase quantitative mass spectrometry paints a pict
259 Pulse-chase radioactive labeling and immunoprecipitation
260 y of IAV-infected cells and biochemically by
pulse-chase radiolabeling experiments.
261 utants and fibroblasts from a human patient,
pulse-chase radiolabeling of newly synthesized proteins
262 Pulse-chase radiolabeling reveals that a ypk1Delta mutan
263 Using
pulse-chase radiolabeling techniques, we find that newly
264 Using
pulse-chase radiolabeling, peptide-N-glycosidase F treat
265 tration, while the half-life was measured by
pulse-chase radiolabelling.
266 also present several lines of evidence (from
pulse-chase,
rapid chemical quench-flow, and stopped-flo
267 kinetic analysis using 14-C tracers and 33-P
pulse-chasing revealed mutation-associated changes in pu
268 We propose that
pulse-chase SILAC labeling is a useful tool for studying
269 findings demonstrate that single time point
pulse-chase SILAC mass spectrometry-based proteomics (pS
270 Using a
pulse-chase SILAC mass spectrometry-based proteomics app
271 oteins in rats were measured in vivo using a
pulse-chase stable isotope labeling experiment.
272 We report here that
pulse-chase stable isotope labeling with amino acids in
273 Using a dual
pulse-chase strategy comparing palmitate and protein hal
274 -wide maps of protein synthesis as well as a
pulse-chase strategy for determining rates of translatio
275 Pulse-chase studies confirmed impaired secretion and inc
276 GH
pulse-chase studies established that the internalized GH
277 Pulse-chase studies found that CYP2E1 protein level is u
278 Classic
pulse-chase studies have shown that actin is conveyed in
279 Pulse-chase studies indicate that PINK1 is rapidly proce
280 Pulse-chase studies of isotopically labeled AChE molecul
281 to 154 decreases pX stability, determined by
pulse-chase studies of WT pX and pX1-140, suggesting tha
282 of rx3 chk mutant/rx3 morphant fish and EdU
pulse-chase studies reveal that rx3 is required to selec
283 Pulse-chase studies revealed that Blebbistatin, a specif
284 Pulse-chase studies showed that osterix-expressing osteo
285 In contrast,
pulse-chase studies with isotope-labeled nutrients revea
286 Here, we report
pulse-chase studies, which unambiguously establish the o
287 izes pVHL, as shown by protein stability and
pulse-chase studies.
288 Pulse-chase study showed that 14-3-3zeta siRNA decreased
289 A biotin
pulse-chase/
subcellular fractionation approach to examin
290 Using a tetracycline-regulated
pulse-chase system, we measured population turnover rate
291 was investigated in perfused rat liver by a
pulse chase technique.
292 nducible histone2B-green fluorescent protein
pulse-chase techniques, we identify a label-retaining ce
293 mational change, a greater amplitude for the
pulse-chase than the pulse-quench reaction, and an activ
294 By using BrdU
pulse-chase to label S-phase cells and follow their prog
295 opic expression followed by cycloheximide or
pulse-chase treatment demonstrated that phospho-mutants
296 a proteome misfolding stress is applied in a
pulse-chase-
type experiment.
297 Using
pulse-chase vital dye labeling to mark newly forming tee
298 In adult mice administered (15)N-thymidine
pulse-chase,
we find that proliferating crypt cells dilu
299 Pulse-chase with immunoprecipitation analyses revealed a
300 Here, we combine H2B-GFP-based
pulse-chasing with cell-surface markers to distinguish q