ライフサイエンスコーパス: purification

1 he need for excess amine reactant or product purification.

2 rement of toxic solvents and chromatographic purification.

3 proteins and we confirmed two of them by co-purification.

4 , liter-scale cultivation, concentration and purification.

5 adverse reactions, and cumbersome production/purification.

6 ents commonly employed for NA extraction and purification.

7 on using recycled hemodialyzers allows water purification.

8 ices (ESs) such as food production and water purification.

9 ins retain procoagulant phospholipid through purification.

10 mics relies on protein digestion and peptide purification.

11 of engineered catalytic materials for water purification.

12 5-42 g L(-1) by direct injection without any purification.

13 main were selected for further expansion and purification.

14 bacterial overexpression system and one-step purification.

15 of cargo delivery, and facilitates Gectosome purification.

16 utilized as a recyclable adsorbent for water purification.

17 in organic chemistry, chemical analysis, and purification.

18 ucted in one-pot tandem without intermediate purification.

19 , thus removing the need for chromatographic purification.

20 roduced within 1 h without elaborate reagent purification.

21 products of a two-step reaction without any purification.

22 res was achieved by feasible chromatographic purification.

23 and do not require column chromatography for purification.

24 with experimental data from membrane protein purification.

25 ernative chromatographic matrix for lysozyme purification.

26 r native sample matrix without enrichment or purification.

27 eractions with native membrane lipids during purification.

28 nvironmental research that pertains to water purification.

29 size exclusion liquid chromatography (FPLC) purification.

30 ring multistep synthesis and chromatographic purification.

31 alt through the carnallite's dehydration and purification.

32 n ideal platform for biopharmaceutical virus purification.

33 w as 0.11% for single reads, without library purification.

34 industrial applications in sour natural gas purification.

35 inding to glycan microarrays and by affinity purification.

36 r steps without the need for chromatographic purification.

37 owed by coimmunoprecipitation and coaffinity purifications.

38 xpands antibody compatibility and simplifies purification, a modified digestion protocol that inhibit

39 r, we demonstrate that even without physical purification, a sophisticated strategy of differential f

40 tion of a standardized phage cultivation and purification across research laboratories participating

41 erials analysis, surface disinfection, water purification, active electromagnetic devices based on ar

42 oquinone) and alumina column chromatographic purification afforded new dithia-bis(calix)-sapphyrins w

43 The purification allowed to recovery three fractions (aqueou

44 Here we review their impact on purification, analysis of pan or chain-selective polyubi

45 We have performed E. coli expression, purification and activity profiling screening of differe

46 rial membrane proteins, OGDs also enable the purification and analysis of a functional G-protein coup

47 Here, we describe the purification and characterization of differentially modi

48 n of GSCs dates back more than a decade, the purification and characterization of GSCs remains challe

49 st approaches that could be valuable for the purification and characterization of other glycoconjugat

50 biochemical approaches were adopted for the purification and characterization of quinoin, a type 1 r

51 ors, but such CDs can still benefit from the purification and characterization procedures outlined in

52 separating solutes from water, such as water purification and desalination.

53 s and electrocatalysis, especially for water purification and environmental remediation applications.

54 Here, purification and extensive mutagenesis revealed protein-

55 After purification and folding, the synthetic materials displa

56 These observations are significant for purification and formulation of rNDV, as exposure to dif

57 Purification and identification of the active compound b

58 Purification and identification revealed deleted in mali

59 The high energy footprint of commodity gas purification and increasing demand for gases require new

60 neration usually involve large-scale protein purification and intensive screening.

61 ens obviating the laborious steps of protein purification and labeling.

62 Using RNA antisense purification and mass spectrometry, we identified up to

63 After purification and mass spectrometry-based characterizatio

64 P1- and RBR-interacting proteins by affinity purification and mass spectrometry.

65 ht the potential of utilizing CNMs for water purification and opens up a simple avenue to creating 2D

66 articles of common materials types, to water purification and optoelectronic devices.

67 Affinity purification and pairwise yeast two-hybrid analysis sugg

68 mposites very promising in the fields of air purification and personal protective equipment.

69 tional tag that allows multidimensional HPLC purification and production of a tagged glycan library,

70 Here, we use vesicle purification and proteomic analysis to identify the endo

71 transport could enable new modes of chemical purification and pumping.

72 type-specific translating ribosome affinity purification and qPCR was used to compare mRNA expressio

73 resis (FFE) has become a useful tool for the purification and real-time analysis of biological mixtur

74 and scFv-induced aggregation can complicate purification and reduce the yield of desired Bipod.

75 combination of translating ribosome affinity purification and ribosome profiling to identify biologic

76 Avoiding excessive chromatographic purification and solid- or liquid-phase supports enabled

77 m was to define an original workflow for the purification and systematic characterization of the mole

78 Here we report a preribosome purification and tagging strategy that overcomes some of

79 This paper describes the purification and the identification of low molecular wei

80 derstanding of the earliest history of water purification and the long-term sustainability of an anci

81 ecture offers the ability to control protein purification and to preserve interactions with native me

82 otif-containing 9 (ARMC9) in tandem-affinity purification and yeast 2-hybrid screens to identify a ci

83 oxidation sequence, minimizing the number of purifications and maximizing the productivity through a

84 percapacitors, electrocatalysts, solar water purification, and atmospheric water harvesting, which sh

85 requires expertise in cell culture, protein purification, and chromatography.

86 characterization using gel electrophoresis, purification, and direct visualization with transmission

87 profiles of cell culture clones, monitoring purification, and evaluating drug substance purity.

88 ncy (i.e. completeness of ligation), ease of purification, and functionality in detergents.

89 published methods for sampling, extraction, purification, and identification/quantification of micro

90 arallel gene cloning, protein expression and purification, and in vivo microcrystal screening for str

91 as energy-intensive processes in synthesis, purification, and isolation.

92 ent challenges in oligonucleotide synthesis, purification, and isolation; highlight potential solutio

93 and automated MagVor homogenization, TruTip purification, and LFA amplification in a multisample, sp

94 oss species, are biochemically unstable upon purification, and possess deeply buried ligand-binding s

95 r common coronaviruses, does not require RNA purification, and takes ~45 min from sample collection t

96 their potential for use in commercial water purification applications.

97 ligases in comparison with one-step affinity purification approaches.

98 Through purification before extraction, the water insoluble curc

99 fabrication much easier without the need for purification but also improved the chip-probing quality.

100 s, trimers, and tetramers of proheads during purification, but only if they had portals.

101 norovirus (HuNoV) is a major focus in water purification, but the effectiveness of disinfection proc

102 Purification by HPLC and analyses of the isolated peaks

103 A sequential two-step purification by size exclusion chromatography was carrie

104 coli cell PCR and one-step LiCl-isopropanol purification can streamline DNA engineering.

105 at integrates cellular engineering, affinity purification, chemical stabilization, and quantitative m

106 We used in vitro DNA affinity purification combined with massively parallel sequencing

107 We applied translating ribosome affinity purification combined with RNA sequencing to characteriz

108 s functional ingredients, bioactive compound purification, composition-activity relationships, and ph

109 herefore, new methods that combine plutonium purification, concentration, and isotopic screening in a

110 By using translating ribosome affinity purification coupled with deep-sequencing (TRAP-seq) in

111 Using immunoaffinity purification coupled with liquid chromatography-high res

112 Using RNA antisense purification coupled with quantitative mass spectrometry

113 -protein interaction assays and DNA affinity purification (DAP) sequencing coupled to in silico predi

114 ts an environmentally benign method of water purification/desalination.

115 c (As(III)) is a central challenge for water purification due to its high toxicity and difficulty to

116 merized to arborisidine upon preparative TLC purification (eluent: MeOH/CHCl(3) saturated with NH(3))

117 nitriles, and sulfides, and was selected for purification experiments.

118 single step with a high yield of 94.3% and a purification factor of 15.7.

119 zyme with a high recovery yield of 98% and a purification factor of 63 in a single step.

120 The percent recovery and purification factor of lysozyme obtained from the chroma

121 For SP-XL adsorbent, the recovery yield and purification factor of lysozyme were 93.78% and ~40 fold

122 adsorbent, lysozyme can be eluted ~100% with purification factor of ~26 folds.

123 Evidence for the oldest known zeolite water purification filtration system occurs in the undisturbed

124 termined using translating ribosome affinity purification followed by high-throughput sequencing.

125 RNA-affinity purification followed by mass spectrometry analysis unco

126 AW112010, we used chromatin isolation by RNA purification, followed by sequencing.

127 ethering or SMOLT, an amplification-free and purification-free molecular assay that can detect microo

128 nrichment of functional RNAs, but also their purification from aggregation-prone "free-riders".

129 nal ribosomes are associated with Fe2+ after purification from cells grown under low O2 and high Fe2+

130 ity of these contaminants are removed during purification, HCPs can represent a considerable risk to

131 Biochemical purification identified histones, both free and bound to

132 ite-directed mutagenesis and lectin affinity purification identified N65 and N82 as bona fide accepto

133 mass spectrometry analysis of dual-affinity purifications identified the USP13 deubiquitinase as a n

134 as limited the use of SFC for separation and purification in the bioanalytical space, especially at t

135 get proteins in cells or light-based protein purification in vitro.

136 rees C, 194 min) and different procedures of purification including alcohol precipitation, ultrafiltr

137 Sensory neuron purification increased numbers of sex-dependent DEGs.

138 However, NSP purification is limited due to high quantities of solubl

139 , rather than sequence-specific nucleic acid purification, is used to pull-down total RNA and crossli

140 gh adsorption capacity and efficiency of the purification levels during repeated adsorption desorptio

141 Our approach combines serial affinity purification, live cell imaging, and cross-linking mass

142 rities and commonly require rigorous reagent purification, low temperatures, and strictly anhydrous r

143 antly metazoan co-fractionation and affinity-purification mass spectrometry experiments.

144 undergoing LTbetaR stimulation and affinity-purification mass spectrometry for the LTbetaR signaling

145 ch of the SARS-CoV-2 proteins using affinity-purification mass spectrometry, identifying 332 high-con

146 Using affinity- purification mass-spectrometry of in vivo interaction pa

147 Affinity purification-mass spectrometry (AP-MS) identified biotin

148 RNA affinity antisense purification-mass spectrometry (RAP-MS) revealed MaIL1 b

149 Affinity purification-mass spectrometry confirmed that 75 protein

150 In this study, quantitative affinity purification-mass spectrometry enabled the delineation o

151 in ATG9A trafficking, we performed affinity purification-mass spectrometry followed by validation of

152 anced Alzheimer's disease; and (ii) affinity purification-mass spectrometry was used to identify whic

153 Here, using tandem affinity purification-mass spectrometry, bioinformatics, and bioc

154 An innovative virus purification material selective for human adenovirus typ

155 , photoelectrodes for water splitting, water purification membranes, and self-cleaning films.

156 the commonly used lysis-centrifugation-based purification method (STEM method) by recovering two spec

157 ides a simple, rapid, and efficient pathogen purification method for significantly improved molecular

158 e results demonstrate the usefulness of this purification method for the dissection of ribosome bioge

159 have used sequence alignments, an anaerobic purification method, iron quantification, and UV-visible

160 loping large-scale (Ci-scale) production and purification methods for (134)Ce.

161 air and poor chromatographic productivity of purification methods.

162 Here, we report a successful expression and purification of a catalytically active recombinant hGFAT

163 The reduced HCP content facilitates purification of a monoclonal antibody.

164 ucture determination that involve multi-step purification of a multi-subunit ubiquitin ligase and che

165 We show that affinity purification of antibodies massively improved the detect

166 Purification of complexes lacking individual components

167 The isolation and purification of Cu(3) P nanoparticles and subsequent sel

168 cept was also demonstrated for offline 2D-LC purification of drug substances.

169 Reciprocal dual-affinity purification of endogenous USP13 followed by mass spectr

170 Purification of ethylene (C(2)H(4)), the largest-volume

171 Purification of EVs from soluble protein was determined

172 e tetrapeptide DYKD as template that affords purification of FLAG-derived recombinant proteins.

173 at allows for the recombinant expression and purification of full-length human FMRP, FXR1P, and FXR2P

174 a cell-surface protein, PLPPR3, that allowed purification of human primitive undifferentiated spermat

175 physical properties within these groups make purification of individual elements a challenge.

176 ghout directed differentiation, enabling the purification of intestinal progenitors and robust genera

177 Purification of islet beta-, alpha- and delta-cells foll

178 able as an alternative to the time-consuming purification of LCs from human skin.

179 physical properties and its application for purification of lysozyme from chicken egg white was inve

180 the strong cationic cryogel was evaluated by purification of lysozyme from egg white.

181 k is the design a cation exchange system for purification of lysozyme from egg-white.

182 The results showed successful purification of lysozyme with a high recovery yield of 9

183 Detergents enable the purification of membrane proteins and are indispensable

184 xamine this, we developed a protocol for the purification of nondenatured mouse CRES, a component of

185 Affinity purification of organelles is a powerful tool for reachi

186 er cycle time and higher sample loading) for purification of pharmaceuticals by selectively switching

187 Here we present a technique for the affinity purification of plant mitochondria (Mito-AP).

188 Technical problems intrinsic to the purification of preribosome intermediates have limited o

189 ve than the homogeneous analogue, allow easy purification of products from the catalyst, are strongly

190 In addition, chromatographic purification of products is avoided, and only a single a

191 we introduce XRNAX, a method for the generic purification of protein-crosslinked RNA, and demonstrate

192 ce ranging from the production, recovery and purification of radioisotopes to the methods used to att

193 logies and analytical techniques used in the purification of such peptides are discussed.

194 nostic assays, we also use ITP for automated purification of target RNA from raw nasopharyngeal swab

195 agents and are associated with difficulty in purification of the by-products.

196 g trisaccharides was acetylated allowing the purification of the main trisaccharide.

197 We reasoned that purification of the nonaggregating, monomeric subpopulat

198 Here, we describe the purification of the nsp1beta:PCBP2:viral RNA complex on

199 l protection step for the easy isolation and purification of the products by chromatography.

200 Upscaling reactions enabled the purification of the respective steroidal alcohols in mod

201 Purification of therapeutic monoclonal antibodies usuall

202 Affinity purification of these complexes, often using antibodies,

203 tandards for the extraction, separation, and purification of these metals from natural sources, recyc

204 Here we describe the expression and purification of various transactivator of transcription

205 and can further guide protein expression and purification optimization.

206 harvest fluid without the need for Protein A purification or other sample preparations and provides u

207 This simple inactivation and purification pipeline is inexpensive and compatible with

208 ass spectrometry method is described for the purification, preconcentration, separation, and characte

209 mids, followed by plasmid DNA extraction and purification prior to downstream PCR-mediated DNA modifi

210 o optimize a receptor's protein sequence and purification procedure, increasing the complexity of the

211 anthocyanins showed similar behavior in all purification procedures performed.

212 Our reactions and purification procedures use no organic solvents, resulti

213 ly analysed the literature on extraction and purification procedures, as well as the main analytical

214 h an analysis is performed following protein purification procedures, which are time consuming, costl

215 eeP is independent of any potentially biased purification procedures.

216 titative information about HCP levels during purification process development.

217 y is applied to demonstrate a postdeposition purification process in which NH(3) is used as a reactan

218 culture fluid (HCCF) materials at different purification/production steps.

219 We used proximity ligation and affinity purification proteomics to identify N-cadherin-binding p

220 To further increase the sensitivity, a purification protocol compatible with this inactivation

221 morillonite and to develop an extraction and purification protocol for its isolation from raw clay sa

222 The inactivation and purification protocol, combined with the RT-LAMP assay,

223 e with Kam's biosynthesis proposal and their purification protocol, we suspect that 19-epi-arborisidi

224 biotin-benzophenone photo-cross-linking and purification protocol.

225 Granulometric extraction and purification protocols increased the montmorillonite con

226 idered artifactual due to the extraction and purification protocols.

227 In vivo RNA Antisense Purification (RAP-MS) identifies YBX1 as a direct intera

228 Currently, nucleic acid (NA) purification remains time-consuming and labor-intensive,

229 The scale-up processes achieved similar purification results, proving linear scalability of the

230 Here we describe serial capture affinity purification (SCAP), in which two separate proteins are

231 A combination of multiple affinity purification screens identified the Clp complex composit

232 Using in vitro DNA affinity purification sequencing (DAP-seq), we identified direct

233 We used translating ribosome affinity purification sequencing and behavioral pharmacology to t

234 Alcohol purification showed that bile acid adsorption is indepen

235 filtration procedure for quinoa 11S globulin purification starting at the bench scale using Ultra15 c

236 The catalyst-free synthesis enabled an easy purification step and recycling of excess monomers.

237 necessary and critical sample preparation or purification step in many lab-on-a-chip diagnostic devic

238 A purification step is recommended for environmental sampl

239 protocol included a simple and rapid extract purification step through polymeric solid phase extracti

240 s in high yields followed with only a single purification step.

241 99.8% purity and over 40% yield in a single purification step.

242 for rapid purification via a single affinity-purification step.

243 No purification steps are necessary.

244 Purification steps guided by ABTS cation radical (ABTS(+

245 was enhanced to 94% by investigating several purification steps, e.g. vacuum distillation and lyophil

246 ut the need for additional wet-chemistry and purification steps.

247 We developed a purification strategy enabling assaying of individual C-

248 The purification strategy was scaled-up to the higher feedst

249 his issue, we developed a new viral affinity purification strategy, Cre-Specific Nuclear Anchored Ind

250 rption is the most economically viable water purification strategy.

251 We develop an HCC EV purification system (i.e., EV Click Chips) by synergisti

252 ma (HCC)-specific extracellular vesicle (EV) purification system for early detection of HCC by perfor

253 for a deployable, cost-effective solar water purification system with assured water quality, especial

254 to provide a sustainable solar-driven water purification system.

255 Placing the only purification tag on the Fab ensured that the isolated En

256 A-protein complex capture by the very common purification tag, 6xHis, but other tags could likely be

257 nctional groups, including reactive handles, purification tags, and removable protecting groups.

258 n results, proving linear scalability of the purification technique adopted.

259 to develop an efficient and reliable exosome purification technique to isolate exosomes from the hete

260 Regardless of purification technique, mitochondria from YAC128 and WT

261 Conventional purification techniques, especially affinity column chro

262 ns, that complements two-hybrid and affinity-purification techniques.

263 conventional antibody discovery and peptide purification techniques.

264 ion, desalination, reverse osmosis for water purification thanks in particular to the emergence of ne

265 tration, we report one-step, liquid-phase NA purification that is simpler and faster than conventiona

266 This study investigated if blood purification therapy with CytoSorb (CS) porous polymer b

267 revisited and modified using mixed-mode SPE purification to adapt the method to the particular physi

268 d protein complexes during and after protein purification to ensure that samples are of sufficient qu

269 heterogeneous nanomaterial solutions without purification to fulfill the regulation requirement on th

270 R-CLASP (VIRal Cross-Linking And Solid-phase Purification) to identify the primary viral RNA-host pro

271 e, we employed translating ribosome affinity purification (TRAP) and RNA sequencing, TRAP-seq, in lar

272 in mice using translating ribosome affinity purification (TRAP) and RNAseq.

273 snRNA-seq) and translating ribosome affinity purification (TRAP) to conduct transcriptomic analyses o

274 e, we combined translating ribosome affinity purification (TRAP) with RNA sequencing to identify mole

275 we adopted a Translational Ribosome Affinity Purification (TRAP)- approach and designed a transgene t

276 PCs, we used translating ribosomal affinity purification (TRAP).

277 om-up analytical approach via immunoaffinity purification, tryptic digestion, and subsequent detectio

278 Since solid-phase purification under protein-denaturing conditions, rather

279 of these vectors for protein expression and purification using a set of 40 target proteins of variou

280 ochondria, enabling their selective affinity purification using magnetic beads coated with Strep-Tact

281 Purification using solid-phase NA extractions utilizes s

282 lex mixtures, without the need for extensive purification, using NMR in tandem with two newly curated

283 a) with a triple hemagglutinin tag for rapid purification via a single affinity-purification step.

284 eveloped viral cross-linking and solid-phase purification (VIR-CLASP) to characterize the earliest in

285 order to assess the proteins' function after purification, we confirmed their binding to pseudoknot a

286 ght source, lead time for stabilization, and purification were investigated for optimization.

287 fferent conditions of protein extraction and purification were tested and the tryptic peptide pools w

288 us for certain applications, such as protein purification, where time and simplicity are critical.

289 ellent functional group tolerance and simple purification, which allows large-scale industrial applic

290 n a mixture sample without any separation or purification, which could not be achieved by the traditi

291 e, we then implemented an iodixanol gradient purification, which resulted in preparations with puriti

292 eplace aquaporins for possible uses in water purification, while concurrently retaining aquaporins' a

293 We here combine this ITP purification with loop-mediated isothermal amplification

294 le by the extraction with sodium citrate and purification with membrane separation, eco-friendly alte

295 Affinity purification with one of the sequences (Sequence 7) comb

296 d(IV) centre, as well as the ease of product purification without column chromatography, render this

297 tional simplicity, ease of product isolation/purification without the aid of tedious column chromatog

298 Low cellular expression, purification yield, and in vitro instability are substan

299 centage and providing a new route to improve purification yields for diagnostic and cellular applicat

300 therefore must be removed at the expense of purification yields.