ライフサイエンスコーパス: purified protein

1 than that displayed in cell-free assays with purified protein.

2 r As(V) uptake was assessed with the His-tag purified protein.

3 mammalian cells, and biophysical analysis of purified protein.

4 c mechanism has been hindered by the lack of purified protein.

5 in vivo to that previously observed for the purified protein.

6 ent from in vitro enzymatic assays using the purified protein.

7 een limited due to difficulties in obtaining purified protein.

8 xtensive biochemical characterization of the purified protein.

9 ractions in cell lysates and in solutions of purified protein.

10 d from assays using recombinantly generated, purified protein.

11 studies of all the A3H variants using highly purified proteins.

12 for conferral of DNA-binding activity on the purified proteins.

13 idine HCl-induced unfolding titrations using purified proteins.

14 hrough high resolution structural studies of purified proteins.

15 and enhanced stability and solubility of the purified proteins.

16 previously been reconstituted in vitro with purified proteins.

17 arm axonemal dynein in motility assays using purified proteins.

18 eractions compared with methods that require purified proteins.

19 ng synthesis in a reticulocyte extract or as purified proteins.

20 zation of pAz on live cells, fixed cells and purified proteins.

21 d spores by comparison with known amounts of purified proteins.

22 of unlabeled ligand and typically use single purified proteins.

23 d by coimmunoprecipitation analyses and with purified proteins.

24 n of protein carbonylation in homogenates or purified proteins.

25 eparations and x-ray crystallography data of purified proteins.

26 om brain tissue and shown to be direct using purified proteins.

27 formation in human cells and in vitro using purified proteins.

28 es from studies with cultured cells and with purified proteins.

29 formation and potential heme transfer using purified proteins.

30 interaction as supported in experiments with purified proteins.

31 try performed on recombinantly expressed and purified proteins.

32 n protein folding in the ER and refolding of purified proteins.

33 fs25) was expressed in Escherichia coli, and purified protein after simple oxidative refolding steps

34 allows a flow-based fractionation of highly purified protein aggregates and simultaneous measurement

35 in mammalian cells when co-nucleofected as a purified protein, along with attB-containing donor plasm

36 nd C10F3.4, respectively) and found that the purified proteins also display GDP-hexose phosphorylase

37 Using purified proteins, an ATPase-defective Cdc6 mutant 'Cdc6

38 Mass spectrometry and purified protein analysis identified, mitochondrial HMG-

39 caftor stimulates the ATPase activity of the purified protein and can compete with the transport of t

40 rd protein immunoblotting procedures on both purified protein and crude nuclear extracts from HEK 293

41 d vitronectin, relative to non-risk, both in purified protein and in cellular models.

42 acterised for their Fab affinity against the purified protein and their Fc affinity to Protein A/G as

43 n vitro lysophospholipid binding assay using purified protein and transport assays with E. coli spher

44 This study demonstrates for purified protein and virus that the NA of the zoonotic H

45 Selected variants were then produced as purified proteins and characterized by surface plasmon r

46 quite different from that seen in studies of purified proteins and cultured cells.

47 this report, using in vitro experiments with purified proteins and DNAs along with DNA endonuclease a

48 T recruitment to viral assembly sites, using purified proteins and giant unilamellar vesicles.

49 lpha-syn and Abeta aggregates in vitro using purified proteins and in a cell culture model that produ

50 With purified proteins and in intact cells, our protein inter

51 Here, using purified proteins and in vitro assays, we define how the

52 ed with the isoforms both in vitro using the purified proteins and in vivo by fluorescence analysis i

53 Using purified proteins and isolated mitochondria, we show her

54 andidates in clinical development are highly purified proteins and peptides relying on adjuvants to e

55 ollowing a minimal systems approach, we used purified proteins and photolithographically patterned me

56 For purified proteins and protein complexes, our workflow us

57 Here, using purified proteins and several biochemical assays and sur

58 In this study, using purified proteins and site-directed mutagenesis, along w

59 Here, using purified proteins and the lipid bilayer system, we chara

60 ants for the reactions, reconstitutions with purified proteins and theoretical modeling to account fo

61 d assay, biochemical characterization of the purified protein, and in vivo complementation, demonstra

62 gns were successful in both lysates and with purified proteins, and that FlAsH binding was dependent

63 towards this phosphosite was confirmed with purified proteins, and this site was required for adapta

64 ces were cross-checked against the masses of purified proteins, and validated by testing the activity

65 mass spectrometry for identification of the purified proteins, and we identify novel gamma-secretase

66 d the development of M1 assembly assays with purified proteins are crucial advances for antiviral tar

67 After capture, the affinity-purified proteins are digested into peptides and compreh

68 have cloned, expressed, and used these three purified proteins as additives in synthetic magnetite pr

69 f Escherichia coli cells expressing LOA1 and purified proteins as enzyme sources.

70 sphorylation of RKIP at serine 153 utilizing purified proteins as well as in cells overexpressing RKI

71 Using a biochemical approach with purified proteins, as well as integration, prespacer pro

72 Application of purified proteins at pH ranges in which PMEI inhibition

73 release that is observed solely in NpHR via purified protein-based assays, demonstrating that indeed

74 econstituted this sophisticated machine with purified proteins, beginning with regulated CMG assembly

75 icating subgenomic replicon and analyzed the purified protein by mass spectrometry.

76 Analysis of the purified protein by size-exclusion chromatography sugges

77 We have characterized the anaerobically purified protein by UV-visible and EPR spectroscopies.

78 the complex with Rrp6-TAP, identified the co-purified proteins by mass spectrometry, and found karyop

79 detailed investigation of cell extracts and purified proteins by top-down MS, NMR spectroscopy, and

80 tro, complexes could be generated using four purified proteins-C3, factor B, factor D, and target pro

81 models have confirmed that brain lysates and purified protein can accelerate brain pathology in a man

82 ) as a model system, we demonstrate that the purified protein can be enzymatically modified with eith

83 Vts1 condensates formed from purified protein can transform naive cells to acquire [S

84 process, based on Anfinsen's discovery that purified proteins can fold on their own after removal fr

85 The purified protein carrying the p.V742G variant showed red

86 richia coli from F(-) toxicity, and that the purified proteins catalyze transport of F(-) in liposome

87 ed for characterization of BSH activity with purified protein, cell lysates, whole cells, and in huma

88 tandem affinity purification produces highly purified protein complexes at high concentrations in a h

89 We purified protein complexes containing biotin-tagged TR2/

90 ticle analysis, determines the structures of purified protein complexes to resolutions sufficient for

91 ons by two-hybrid techniques and of affinity-purified protein complexes.

92 onstituting the NOCAP synthase in vitro from purified protein components followed by heterologous exp

93 e has never been reconstituted in vitro from purified protein components.

94 nal flexibility measured experimentally with purified protein components.

95 , a majority of these techniques need to use purified protein, concentrated enough in the solution to

96 Intrinsic fluorescence spectroscopy of purified proteins confirmed a stronger binding reduction

97 In vitro kinetic analysis using purified protein demonstrated that the inhibition of 2-A

98 Immunoprecipitation and pulldown assays of purified proteins demonstrated a direct interaction betw

99 s were cocultured with autologous T-Regs and purified protein derivative (PPD) preprimed T-Reg-deplet

100 tality while inducing more BCG reactions and purified protein derivative (PPD) responses (secondary o

101 tality while inducing more BCG reactions and Purified Protein Derivative (PPD) responses (secondary o

102 ic IgG, bulk Fc domain, bulk Fab domain, and purified protein derivative (PPD)- and Ag85A-specific Ig

103 nocyte-derived macrophages co-incubated with purified protein derivative (PPD)-driven peripheral bloo

104 Purified protein derivative (PPD)-specific immune recove

105 o Toll-like receptor (TLR)-2, -4 or -7/8, or purified protein derivative (PPD).

106 ntigen and 2 tuberculosis-specific antigens (purified protein derivative [PPD] and ESAT-6/CFP10), fol

107 However, 48 h after tuberculin purified protein derivative administration in the ipsila

108 Purified protein derivative ELISPOT responses increased

109 in skin test, and (4) Battey skin test using purified protein derivative from the Battey bacillus.

110 Interestingly, whereas purified protein derivative induced such cells in both L

111 increased the in vitro cytokine responses to purified protein derivative of Mycobacterium tuberculosi

112 Polyclonal and purified protein derivative of tuburculin-specific T-cel

113 en both vaccines were administered together, purified protein derivative responses were enhanced in f

114 Cellular responses to purified protein derivative stimulation did not differ b

115 f IFN-gamma ELISPOT responses to recall Ags (purified protein derivative, Tetanus toxoid, or flu/EBV/

116 gamma release was significantly reduced when purified protein derivative- and Ag85B-specific CD4(+) T

117 The production of M. tuberculosis and purified protein derivative-induced IFN-gamma, TNF-alpha

118 absence of anti-LipC antibodies in sera from purified protein derivative-positive (PPD+) healthy subj

119 Immunization with BCG generated low purified protein derivative-specific CD4 T cell response

120 We observed that purified protein derivative-specific human CD4(+) T lymp

121 e stimulated with DEP and M. tuberculosis or purified protein derivative.

122 able to show that recombinantly produced and purified protein does not bind any known phytochrome chr

123 The purified protein exhibits a "hyper-helicase" unwinding a

124 Remarkably, cell extract and purified protein experiments show that MB induces disass

125 ure that will yield sufficient quantities of purified protein for intrinsic UV absorbance analysis an

126 analyzed expression media, cell lysates, and purified proteins for FXII activation.

127 f some industrial lupin protein isolates and purified protein fractions were tested.

128 -supplied exogenously as genetic elements or purified protein fragments-had no significant catalytic

129 of intact cells, rat brain homogenates, and purified protein fragments.

130 Purified protein from Escherichia coli showed robust sta

131 titute Dmc1 recombination in vitro using six purified proteins from budding yeast including Dmc1 and

132 We show here, using purified proteins from budding yeast, that Dpb11 alone b

133 We show in this manuscript, using purified proteins from budding yeast, that Mcm10 directl

134 a parS-dependent ParB spreading event using purified proteins from Caulobacter crescentus and show t

135 LCMS proteomic analysis of LAP-TAP-purified proteins from HeLa cells containing a tetracycl

136 Here, in a series of experiments using purified proteins from mammalian cells, bacteria, and a

137 reconstitute dynein plus-end transport using purified proteins from S. cerevisiae and dissect the mec

138 Here, we address this issue using purified proteins from Saccharomyces cerevisiae and a co

139 Using purified proteins from Saccharomyces cerevisiae, we have

140 Using a reconstituted system with purified proteins from Saccharomyces cerevisiae, we show

141 ction of X-ray fluorescence signals for 3879 purified proteins from several hundred different protein

142 Examples include purified proteins from the alpha-hemolysin pore from Sta

143 ion of DNA replication free in solution with purified proteins from the budding yeast Saccharomyces c

144 maturation was reconstituted in vitro using purified proteins from Thermococcus species 9 degrees N

145 onstituted system containing genomic DNA and purified proteins from yeast, Krietenstein et al. uncove

146 xogenous fatty acid supplementation, and the purified protein had less than 5% of the enzymatic activ

147 SDS-PAGE showed that the purified protein had molecular weight (24kDa) in concord

148 X-ray structural analysis of the purified protein has revealed that the dimer is held tog

149 Whereas MGs of various purified proteins have been probed to date, no data are

150 s isolated following in vitro interaction of purified proteins (hCtf4 plus the hCMG complex), coinfec

151 Using purified protein, here we show that the heterogeneous nu

152 specific interactions were detected even for purified proteins, highlighting the importance of correc

153 The purified proteins hydrolyzed the enamines/imines formed

154 mice and analytic size exclusion studies of purified proteins identify that interactions between cyc

155 donuclease can be introduced into cells as a purified protein in complex with a single guide RNA (sgR

156 The purified protein in detergent showed a weak basal ATPase

157 8 independent of environment, whether as the purified protein in solution or expressed in live cells.

158 limited due to inability of obtaining stable purified protein in sufficient quantities.

159 When recapitulated with purified protein in vitro, this modification completely

160 p, in part because it is difficult to obtain purified protein in well defined lipid systems.

161 Here we use purified proteins in a reconstituted system and in vivo

162 refore, structural studies are restricted to purified proteins in vitro and these findings are extrap

163 s to complement activation peptide C5a using purified proteins in vitro, and by ex vivo depletion of

164 s and reconstituted assays of resection with purified proteins in vitro, we show that DNA-dependent p

165 SP2 both as a Y2H interaction in vivo and as purified proteins in vitro.

166 Reaction of the purified protein, in detergent, with the thiol-reactive

167 s of Pot1a-Tpt1-Pat1 complex formation using purified proteins indicated that Tpt1 interacts directly

168 The purified protein inhibited mitogen-activated proliferati

169 When modeled in HEK293 cells and with purified protein, IRE1beta diminishes expression and inh

170 We show that the purified protein is a fully functional ion channel with

171 The expressed and purified protein is properly folded and has increased al

172 Because pegylation of purified proteins is commonly used as a method to increa

173 nant rat DAT N-terminal peptide (NDAT) using purified protein kinases.

174 The purified protein likely exists in a monomer-dimer equili

175 Bioluminescence can be detected from the purified protein, live Drosophila Schneider 2 cells, and

176 lerated when compared to the dissociation of purified proteins measured in vitro.

177 The purified protein mediates Ca(2+)-dependent Cl(-) transpo

178 ractions cannot be studied using traditional purified protein methods, demonstrating the importance o

179 ter intranasal immunization of mice with the purified proteins mixed with the Th17 adjuvant curdlan,

180 Here we use purified proteins, mouse primary neurons and patient-der

181 n the minimal PURE system, which consists of purified proteins necessary for transcription and transl

182 The higher yields of purified proteins obtained with constructs lacking the l

183 obustly than their wild-type parent, and the purified protein of those mutants showed a decrease in c

184 CD45 from bound receptor-ligand pairs using purified proteins on model membranes.

185 ilable probes are most effectively used with purified protein or cell lysate.

186 Most previous studies on NM II used tissue-purified protein or expressed fragments of the molecule,

187 c nanobodies, known as sybodies, against any purified protein or protein complex within a 3-week peri

188 uctase (GR) inhibitors, directly against the purified proteins or in cell extracts.

189 f sugar nucleotidyltransferase activities of purified proteins or in cell lysates using a mass-differ

190 t screening allowing to obtain tens of mg of purified protein per liter of culture.

191 aled ERK1/2 as a positive regulator, whereas purified protein phosphatase 1 (PP1), dephosphorylated T

192 ry structure and oligomeric state using both purified protein preparations and in-cell NMR on E. coli

193 o directly screen structural features of pre-purified proteins, protein complexes or clarified cell l

194 osomes containing functionally reconstituted purified protein provided strong evidence for active eff

195 riments in livers of mice and in vitro using purified proteins provided evidence that PNPLA3 and CGI-

196 of antibodies elicited by immunization with purified protein provides strong support for further eva

197 The SERS signal from the purified proteins provides basis spectra to analyze the

198 h different cysteine oxidation states of the purified proteins, providing a link between translation,

199 ion of ETS2 and MESP1 or cell treatment with purified proteins reprograms fibroblasts into cardiac pr

200 ion of promoter nucleosome organization with purified proteins resolves this problem and is therefore

201 on, localization and transport function, and purified proteins retained drug stimulation of ATP hydro

202 An initial spectroscopic characterization of purified protein revealed that the photocycle and the tr

203 Binding studies using purified proteins revealed that FX binds specifically (h

204 otypic analyses and biochemical studies with purified proteins revealed that the mono-haem c-type cyt

205 ST fusion proteins or GST-free counterparts, purified proteins revealed that the PX domain is suffici

206 Compared to purified protein samples, we show that pretreatment of c

207 subunits, and the UV-visible spectrum of the purified protein showed absorbance peaks at 380 and 460

208 e LXR4 was heterologously expressed, and the purified protein showed high specificity for L-xylo-3-he

209 Enzymatic assays using purified protein showed that unlike OdaA, which did not

210 The purified proteins showed similar kinetic behavior and co

211 In vitro binding assays using purified proteins showed strong affinity for the substra

212 The tests carried out using purified protein solution and clinical serum samples dep

213 hniques to measure polymer concentrations in purified protein solutions, but few are applicable in vi

214 N- and C-termini has been limited to single purified protein species, while the proteome-wide identi

215 Here, using recombinantly expressed and purified proteins, static light-scattering analysis, and

216 agonize one another, and binding assays with purified proteins suggest that the association of RelA a

217 S loading and time-resolved FRET assays with purified proteins suggested that l-PGDS enhances GDP-GTP

218 t plasmids containing pre-RCs assembled with purified proteins support complete and semi-conservative

219 Using purified proteins, surface plasmon resonance, and report

220 In a purified protein system, FXI is activated by beta-thromb

221 ecific pAz ligation and CuAAC on cells or on purified proteins takes 40 min-3 h.

222 igand screening or selection methods using a purified protein target.

223 pathway activity, and controlling binding to purified protein targets in vitro.

224 ges for generating affinity reagents against purified protein targets, and we have now significantly

225 e utilized, and new biochemical studies with purified proteins that are aimed at reconstituting leadi

226 e fluorophores, we generated living cells or purified proteins that bind but do not transport target

227 We show using purified proteins that HDHB physically interacts with th

228 We show here using purified proteins that in the presence of SSB and RecA,

229 We also demonstrate using purified proteins that MAP6-F binds directly to microtub

230 We show using purified proteins that Siah2 acts as an E3 ligase to dir

231 ing site for Rap1A, we first confirmed using purified proteins that the RA2 domain is indeed essentia

232 In addition to purified protein, the proposed immunosensor effectively

233 l recent efforts toward reconstituting, with purified proteins, the basic structural motifs that recu

234 umen formation can be rescued by addition of purified protein to knockdown cultures.

235 on studies in cells and on direct binding of purified protein to lipids.

236 We used purified proteins to assess interaction of the cytoplasm

237 Finally, we use purified proteins to reconstitute LC3- and JMY-dependent

238 a wide variety of preparations, ranging from purified proteins to reconstituted eukaryotic cell membr

239 We used yeast proteome microarrays (~5800 purified proteins) to conduct a high-throughput and syst

240 Experimental spectroscopy studies with purified proteins validate our MD predictions and corrob

241 his study, using recombinantly expressed and purified proteins, various analytical methods, in vitro

242 The purified protein was a dimer whose dimer interface invol

243 The purified protein was also characterized with 1.3-fold hi

244 Further, glycation of this purified protein was carried out.

245 The expressed, purified protein was found to behave similarly to other

246 The purified protein was shown to be activated in a pH-depen

247 The purified protein was shown to be active with fatty acyl-

248 The purified protein was shown to be properly folded with st

249 The purified protein was then chemically modified with the b

250 This confirmed that the purified protein was trypsin.

251 as a lethal mutation in single copy, and the purified protein was unable to transfer biotin from enzy

252 One of the purified proteins was identified as tenascin-R (TNR) by

253 Using a steady-state kinetics approach with purified proteins we demonstrate that eIF2B binds to eIF

254 Using purified proteins we found that arrestin-3 directly bind

255 Here, using purified proteins we quantitated the binding of HIV-1 Ga

256 Using purified proteins we show that arrestin-3 directly inter

257 Using purified proteins we show that DNA translocases, includi

258 Using purified protein, we report that human FANCA has intrins

259 Using purified proteins, we addressed the mechanistic defects.

260 Using purified proteins, we also found that PTP1B is relativel

261 Using purified proteins, we also show that the PARP1-XPC compl

262 Using purified proteins, we confirm that RPA inhibits A3A by b

263 By using purified proteins, we confirmed the direct interaction,

264 Using a minimal set of purified proteins, we create droplets of cross-linked bi

265 d surface plasmon resonance experiments with purified proteins, we demonstrate that ADAMTS9 and fibro

266 Using biochemical reconstitution with purified proteins, we demonstrate that Rho-GDP promotes

267 Using natively purified proteins, we demonstrate that the pyruvate and

268 Using purified proteins, we demonstrated that each VP24 binds

269 Finally, using purified proteins, we find that Hrr25 phosphorylates the

270 Using purified proteins, we find that PLCbeta binds approximat

271 Using purified proteins, we found that Abl2 binds growing MTs

272 Using purified proteins, we found that DnaD inhibited the abil

273 Finally, using purified proteins, we found that MRN could stimulate bot

274 mass spectrometry and mutational analysis of purified proteins, we found that Set7/9 methylates the N

275 time binding of recombinantly-expressed and -purified proteins, we found that W110(PD-L2) acts as an

276 When the pathway was reconstituted with purified proteins, we observed the formation of four acy

277 Using purified proteins, we reconstitute the regulation of the

278 Using purified proteins, we report a minimal cell-free system

279 ammalian cells, Xenopus nuclear extracts and purified proteins, we show that after DNA damage, PCNA l

280 Using purified proteins, we show that although DEP1 phosphoryl

281 Using purified proteins, we show that Sgs1, Top3, Rmi1, and re

282 Using purified proteins, we show that the replicative polymera

283 Using purified proteins, we showed that SATB1 interacts direct

284 ys with both mammalian cell-free extract and purified proteins, we unexpectedly discovered that lesio

285 rowth, and the biochemical properties of the purified protein were indistinguishable from those of Fa

286 The methyltransferase activities of the purified proteins were analyzed by methyl incorporation

287 The purified proteins were analyzed for the presence of lysi

288 Purified proteins were assessed for V3 loop binding usin

289 Purified proteins were evaluated for secondary structure

290 When the purified proteins were mixed, they became strongly but r

291 precursor protein, were identified, and the purified proteins were shown to inhibit the phagocytosis

292 Purified proteins were subjected to size exclusion chrom

293 stimulated A3G deamination activity when the purified proteins were used in in vitro reactions.

294 nteractions with signaling phospholipids use purified proteins, which do not take into account the ef

295 in detail and recapitulated it in vitro with purified proteins, which suggests direct interaction.

296 d to site-specifically label cell-surface or purified proteins with chemical probes in two steps: pro

297 We have combined X-ray crystallography of purified proteins with electron cryotomography of native

298 immunoblotting and by digesting recombinant/purified proteins with exogenous MMPs.

299 ing only five purification steps with a high purified protein yield (160 mg LTP1 and LTP1b from 200 g

300 d crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyc