ライフサイエンスコーパス: pyridoxal phosphate

1 do not form heterotetramers, and do not bind pyridoxal phosphate.

2 e pyrroline ring with the aldehyde carbon of pyridoxal phosphate.

3 nting stable formation of a Schiff base with pyridoxal phosphate.

4 by catalyzing incorporation of its cofactor, pyridoxal phosphate.

5 idue interacts with the pyridine nitrogen of pyridoxal phosphate.

6 s form external aldimines with the coenzyme, pyridoxal phosphate.

7 The enzyme contains covalently bound pyridoxal phosphate.

8 to stabilize the 3'O- functionality of bound pyridoxal phosphate.

9 ition about 8 angstrom from the phosphate of pyridoxal phosphate.

10 of aldolases whose activity is dependent on pyridoxal phosphate.

11 nd the nonselective P2 receptor antagonists, pyridoxal phosphate 6-azophenyl-2',4'-disulfonic acid an

12 rents were blocked by 100 microm suramin and pyridoxal phosphate 6-azophenyl-2',4'-disulfonic acid.

13 The P2Y receptor antagonists suramin and pyridoxal phosphate 6-azophenyl-2'-4'-disulfonic acid bo

14 Pyridoxal phosphate 6-azophenyl-2,4'-disulfonic acid, an

15 ythroidine and reduced by the P2X antagonist pyridoxal phosphate-6-azo (benzene-2,4-disulfonic acid (

16 r ATP, and by blocking P2 purinoceptors with pyridoxal phosphate-6-azo(benzene-2,4-disulfonic acid) t

17 PPADS [pyridoxal phosphate-6-azo(benzene-2,4-disulfonic acid)],

18 ts were potentiated by low concentrations of pyridoxal phosphate-6-azophenyl-2', 4'-disulfonic acid a

19 Suramin and pyridoxal phosphate-6-azophenyl-2', 4'-disulfonic acid w

20 osine-3', 5'-bisphosphate (K(i)=900 nM), and pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (K

21 ed in low divalent cation medium, blocked by pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (P

22 ramin (100 micromol/L) or the P2X antagonist pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (P

23 as blunted after the P2X receptor antagonist pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (P

24 e) inhibition by Brilliant Blue G, Cu2+, and pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid te

25 (1 microM), but persisted in the presence of pyridoxal phosphate-6-azophenyl-2',4'-disulphonic acid (

26 ion of the P2 purinergic receptor antagonist pyridoxal phosphate-6-azophenyl-2',4'-disulphonic acid (

27 5-na phthalene-trisulphonic acid) and PPADS (pyridoxal phosphate-6-azophenyl-2',4'-disulphonic acid).

28 Hindlimb intra-arterial infusion of pyridoxal phosphate-6-azophenyl-2,4-disulfonic acid (PPA

29 Pyridoxal-phosphate-6-azophenyl-,2',4'-disulphonic acid

30 eptor antagonists periodate-oxidized ATP and pyridoxal-phosphate-6-azophenyl-2', 4'-disulfonic acid,

31 adult rats show that bilateral injections of pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS

32 blocked by P2Y receptor antagonists suramin, pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS

33 The ENPP1 inhibitor pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS

34 s with the broad-spectrum antagonists PPADS (pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate) or RB

35 by the antagonists suramin (300 microM) and pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid (3

36 e sensitivity to the antagonists suramin and pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid (P

37 d by a pyridoxal-phosphate-based antagonist (pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid) o

38 was blocked by the P(2) receptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

39 P2 receptor antagonists suramin (0.02 M) and pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

40 by 97 +/- 2% by the P2X receptor antagonist, pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

41 blocked by the broad P2 receptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

42 blocked by the broad P2 receptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

43 ed to MA; (iii) the P2X receptor antagonist, pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

44 yrrhetinic acid, blocking ATP receptors with pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

45 Reactive Blue 2 and pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid i

46 e or no effect on sensitivity to suramin and pyridoxal-phosphate-6-azophenyl-2,4-disulfonate in chime

47 r, which is inhibited by NF449, suramin, and pyridoxal-phosphate-6-azophenyl-2,4-disulfonate, with re

48 ), which forms an internal aldimine with the pyridoxal phosphate and catalyzes the abstraction of the

49 unusual in its dependence on two cofactors: pyridoxal phosphate and heme.

50 a-His(86) plays a structural role in binding pyridoxal phosphate and in stabilizing the correct orien

51 The presence of pyridoxal phosphate and pyridoxal as ligands was confirm

52 The results of this screen identified pyridoxal phosphate and pyridoxamine phosphate as equili

53 al to a compound previously synthesized from pyridoxal phosphate and pyruvate.

54 s of very slow biological reactions, notably pyridoxal phosphate and the ceric ion, are shown to meet

55 ny chromogenic cofactor, and the presence of pyridoxal phosphate and the mechanistically related pyru

56 interaction of His(86) with the phosphate of pyridoxal phosphate and with Lys(87) lowers the pK(a) of

57 Unexpectedly, the double bond linking the pyridoxal-phosphate and benzoate moieties was reduced by

58 NAD, pyridoxal phosphate, and coumermycin A1, which exhibit n

59 en fasting plasma levels of vitamin B(6), as pyridoxal phosphate, and subsequent myocardial infarctio

60 xygen-sensitive and biotin-, ATP-, thiamin-, pyridoxal phosphate-, and metal-ion-independent, reversi

61 The pyridoxal phosphates appear to be primarily present as g

62 This E. coli protein contains pyridoxal phosphate as a cofactor and catalyzes the remo

63 as a molecular weight of 45 kDa and contains pyridoxal phosphate as a cofactor.

64 hould be measured, and therapeutic trials of pyridoxal phosphate as well as pyridoxine should be cons

65 The protein contained a highly conserved pyridoxal phosphate attachment site in the C-terminal do

66 ed the serine/threonine dehydratase types of pyridoxal-phosphate attachment site.

67 LPS-induced iNOS expression is mimicked by a pyridoxal-phosphate-based antagonist (pyridoxal-phosphat

68 isomal aggregation (Gly41Arg), inhibition of pyridoxal phosphate binding and loss of catalytic activi

69 cement of His(86) by leucine (H86L) weakened pyridoxal phosphate binding approximately 20-fold and ab

70 DhpH is a multidomain protein, in which a pyridoxal phosphate binding domain is fused to an N-acet

71 Mutations in the pyridoxal phosphate binding site of the tryptophan synth

72 at several key residues directly involved in pyridoxal phosphate binding were strictly conserved.

73 CBS is a modular protein with a heme and pyridoxal phosphate-binding catalytic core, which is sep

74 acid sequence of CobD identified a consensus pyridoxal phosphate-binding motif.

75 for in vitro growth, and encodes a putative pyridoxal phosphate-binding protein of unknown function.

76 o acid sequence of CGS1 contains a consensus pyridoxal phosphate-binding site and is similar to MetB

77 amer comprising two obligate dimers and four pyridoxal phosphate-bound active sites.

78 8 to participate in nucleophilic attack of a pyridoxal phosphate-bound cysteine substrate.

79 studies of the enzyme because the different pyridoxal phosphate-bound intermediates can be followed

80 lyzes the second step in the biosynthesis of pyridoxal phosphate by oxidizing 4-phospho-d-erythronate

81 tational study of nonenzymatic and enzymatic pyridoxal phosphate-catalyzed decarboxylation of 2-amino

82 aldolase activity is an intrinsic feature of pyridoxal phosphate chemistry and identifies principles

83 ne forms a stable external aldimine with the pyridoxal phosphate coenzyme at the active site of the b

84 tated by the spectroscopic properties of the pyridoxal phosphate coenzyme that forms a series of inte

85 tated by the spectroscopic properties of the pyridoxal phosphate coenzyme.

86 e provides information on the binding of the pyridoxal phosphate cofactor as well as on amino acid re

87 ocated on a helix that connects the heme and pyridoxal phosphate cofactor domains.

88 ases via reactions of the compounds with the pyridoxal phosphate cofactor forming an irreversible add

89 interacts with the pyridine nitrogen of the pyridoxal phosphate cofactor from a neutral Ser (beta-Se

90 L-Allothreonine reacts with the pyridoxal phosphate cofactor to form a stable 3-methyl a

91 ing strain of E. coli and shown to contain a pyridoxal phosphate cofactor, as judged by ultraviolet/v

92 s328 is positioned greater than 17A from the pyridoxal phosphate cofactor, suggesting that a large co

93 ond of the serine side-chain mediated by the pyridoxal phosphate cofactor.

94 PtaA is homodimeric and contains a pyridoxal phosphate cofactor.

95 of Cys328 to within approximately 3A of the pyridoxal phosphate cofactor.

96 A of the glycine alpha-carbon of the glycine-pyridoxal phosphate complex; the complex appears to be p

97 Folate, vitamin B12, and pyridoxal phosphate concentrations were also determined.

98 Residual activity of K165M is insensitive to pyridoxal phosphate, confirming K165 as the target of th

99 r and UV-visible spectra characteristic of a pyridoxal phosphate containing enzyme.

100 Like NifS, IscS is a pyridoxal-phosphate containing homodimer.

101 We recently discovered that the pyridoxal phosphate-containing enzyme PvdN is responsibl

102 NIFS protein from Azobacter vinelandii is a pyridoxal phosphate-containing homodimer that catalyzes

103 e enzyme binds the substrate cysteine in the pyridoxal phosphate-containing site, and a persulfide is

104 rnal aldimine form of NtdA with the cofactor pyridoxal phosphate covalently attached to Lys-247.

105 The pyridoxal phosphate dependent alanine racemase catalyzes

106 c stressor capable of inactivating important pyridoxal phosphate dependent enzymes in a cell.

107 ucose-6-phosphate 3-dehydrogenase, NtdA is a pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:

108 ylacetaldehyde (4-HPAA), Rhodiola contains a pyridoxal phosphate-dependent 4-HPAA synthase that direc

109 at the primary amino acid level with several pyridoxal phosphate-dependent amino acid decarboxylases.

110 The S-signal was generated by the pyridoxal phosphate-dependent aminotransferase ScrA; sig

111 ine beta-synthase found in yeast catalyzes a pyridoxal phosphate-dependent condensation of homocystei

112 Sequence homology identified the pyridoxal phosphate-dependent decarboxylase-like protein

113 ts that it may be a member of a new class of pyridoxal phosphate-dependent decarboxylases.

114 nzymological source of the amine moiety as a pyridoxal phosphate-dependent decarboxylating enzyme tha

115 uted cysteine desulfurase that catalyzes the pyridoxal phosphate-dependent desulfuration of L-cystein

116 The pyridoxal phosphate-dependent enzyme 1-aminocyclopropane

117 The catalytic effects of perdeuterating the pyridoxal phosphate-dependent enzyme alanine racemase fr

118 osynthesis of L-beta-ethynylserine where the pyridoxal phosphate-dependent enzyme BesB forms a rare t

119 S. cerevisiae FKF had been reported to be a pyridoxal phosphate-dependent enzyme encoded by BNA3.

120 Cystathionine gamma-synthase (CGS) is a pyridoxal phosphate-dependent enzyme that catalyzes a ga

121 Cystathionine beta-synthase (CBS) is a pyridoxal phosphate-dependent enzyme that catalyzes the

122 oxymethyltransferase (SHMT) is a tetrameric, pyridoxal phosphate-dependent enzyme that catalyzes the

123 Serine hydroxymethyltransferase (SHMT) is a pyridoxal phosphate-dependent enzyme that catalyzes the

124 e aminotransferase (GABA-AT) is a tetrameric pyridoxal phosphate-dependent enzyme that catalyzes tran

125 The pyridoxal phosphate-dependent enzyme, 1-aminocyclopropan

126 te, which is converted to Ala(P) by a second pyridoxal phosphate-dependent enzyme, DhpD.

127 site of cystathionine beta-synthase (CBS), a pyridoxal phosphate-dependent enzyme.

128 Herein we systematically mine pyridoxal phosphate-dependent enzymes (PLP-DEs) in bacte

129 understanding of the catalytic repertoire of pyridoxal phosphate-dependent enzymes and will enable de

130 rboxylate (ACC) synthase as well as of other pyridoxal phosphate-dependent enzymes.

131 a sugar aminotransferase that catalyzes the pyridoxal phosphate-dependent equatorial transamination

132 s study reassigns MilM as an oxygen (O(2))-, pyridoxal phosphate-dependent hydroxylase that hydroxyla

133 Alanine racemase catalyzes the pyridoxal phosphate-dependent interconversion of the D-

134 vivo and in vitro enzyme assays, supports a pyridoxal phosphate-dependent mechanism of Sec-tRNA(Sec)

135 y Sep-tRNA:Cys-tRNA synthase (SepCysS) via a pyridoxal phosphate-dependent mechanism.

136 erature, and of isotopic substitution on the pyridoxal phosphate-dependent reaction of L-serine with

137 and homocysteine to form cystathionine in a pyridoxal phosphate-dependent reaction.

138 steine, a toxic metabolite, with serine in a pyridoxal phosphate-dependent reaction.

139 Monovalent cations activate the pyridoxal phosphate-dependent reactions of tryptophan sy

140 Among them, we identified the L136 gene as a pyridoxal phosphate-dependent sugar aminotransferase.

141 c acid aminotransferase (BioA) catalyses the pyridoxal phosphate-dependent transamination of 7-keto-8

142 The O(2)-, pyridoxal phosphate-dependent transformation of l-argini

143 Conversion of l-dopa to dopamine by a pyridoxal phosphate-dependent tyrosine decarboxylase fro

144 e and that AGXT2L1 and AGXT2L2 catalyzed the pyridoxal-phosphate-dependent breakdown of phosphoethano

145 Cystathionine beta-synthase (CBS) is a pyridoxal-phosphate-dependent enzyme that catalyzes a be

146 three-gene operon that potentially encodes a pyridoxal-phosphate-dependent enzyme, an extracellular s

147 1 and AGXT2L2, two closely related, putative pyridoxal-phosphate-dependent enzymes encoded by vertebr

148 not necessarily required for recognition of pyridoxal phosphate derivatives.

149 homogeneous enzyme were characteristic of a pyridoxal phosphate enzyme and showed the absence of hem

150 (EC 2.1.2.1), a member of the alpha-class of pyridoxal phosphate enzymes, catalyzes the reversible in

151 which belong to two different fold types of pyridoxal phosphate enzymes: an aspartate aminotransfera

152 anistic studies showed that the enzyme-bound pyridoxal phosphate forms a Schiff's base with the amino

153 cular terms together with the earlier solved pyridoxal phosphate forms of the enzyme.

154 plants, the pathway for de novo synthesis of pyridoxal phosphate has been well characterized, however

155 In contrast, pyridoxal phosphate (IC50 = 18 microM and coumermycin A1

156 PRK-K53M retains sensitivity to pyridoxal phosphate, implicating K165 as the target of t

157 nd in stabilizing the correct orientation of pyridoxal phosphate in the active site of the beta subun

158 t the active site lysine that normally binds pyridoxal phosphate in the cSHMT protein is exposed to s

159 nd to interact with the pyridine nitrogen of pyridoxal phosphate in the structure of alanine racemase

160 imelate epimerase is a representative of the pyridoxal phosphate-independent amino acid racemases, fo

161 To study the physiological role of Ala AT, a pyridoxal phosphate inhibitor, aminooxyacetic acid, was

162 to form L-cystathionine through a series of pyridoxal phosphate intermediates.

163 The requirement for pyridoxal phosphate is expected on the basis of the natu

164 Pyridoxal phosphate is the cofactor for the enzyme histi

165 Vitamin B6 (pyridoxal phosphate) is an essential cofactor in enzymat

166 y X-rays to create a covalent linkage of the pyridoxal-phosphate moiety to lysine 120 in the binding

167 se is a unique heme protein that catalyzes a pyridoxal phosphate (or PLP)-dependent beta-replacement

168 ositions Thr313 for its interaction with the pyridoxal phosphate oxygens and substrate alpha-carboxyl

169 ciated with plasma folate, vitamin B-12, and pyridoxal phosphate (P for trend < 0.001).

170 ervation that the active form of vitamin B6 (pyridoxal phosphate, P5P) modulates the self-assembly of

171 e one, are dimers containing one molecule of pyridoxal phosphate per subunit.

172 e vitamin B-6 vitamer composition of plasma [pyridoxal phosphate (PLP) > pyridoxic acid (PA) > pyrido

173 olated and recombinant enzymes are devoid of pyridoxal phosphate (PLP) and are rapidly inactivated up

174 It is unique in being dependent on both pyridoxal phosphate (PLP) and heme for activity.

175 Plasma pyridoxal phosphate (PLP) and pyridoxal concentrations a

176 SHMT requires both pyridoxal phosphate (PLP) and tetrahydropteroylpolygluta

177 structures reveal that covalent adducts with pyridoxal phosphate (PLP) are formed in the active site

178 transaminase is a bacterial enzyme that uses pyridoxal phosphate (PLP) as a cofactor to catalyze the

179 Nonenzymatic pyridoxal phosphate (PLP) catalyzed decarboxylations and

180 We show SulA has a bound pyridoxal phosphate (PLP) cofactor that undergoes a spec

181 Heme and pyridoxal phosphate (PLP) cofactors are necessary to cat

182 The pyridoxal phosphate (PLP) dependent enzyme dialkylglycin

183 alue of this approach for fingerprinting the pyridoxal phosphate (PLP) enzyme mechanism.

184 D-Amino acid transaminase, a pyridoxal phosphate (PLP) enzyme, is inactivated by its

185 ysts represented the key to enable efficient pyridoxal phosphate (PLP) enzyme-catalyzed radical react

186 I reflect on my research on pyridoxal phosphate (PLP) enzymes over fifty-five years

187 Pyridoxal phosphate (PLP) exhibited similar behavior to

188 oxygen is activated by the organic cofactor pyridoxal phosphate (PLP) for oxidation reactions remain

189 ve been determined crystallographically: the pyridoxal phosphate (PLP) form and a complex with the re

190 and identified PDXK-an enzyme that produces pyridoxal phosphate (PLP) from vitamin B6-as an acute my

191 ridoxal 5'-phosphate and failure to maintain pyridoxal phosphate (PLP) levels results in epilepsy.

192 enzyme that requires the cofactors heme and pyridoxal phosphate (PLP) to catalyze the condensation o

193 f its active site histidine and lysine-bound pyridoxal phosphate (PLP) to the thiamin pyrimidine (HMP

194 their cofactors (the pterins and vitamin B6 (pyridoxal phosphate (PLP))) in human cerebrospinal fluid

195 Baseline serum riboflavin, pyridoxal phosphate (PLP), folate, vitamin B12, and flav

196 CAS and the Gm-CAS K95A mutant with a linked pyridoxal phosphate (PLP)-Cys molecule in the active sit

197 a-synthase is a hemeprotein that catalyzes a pyridoxal phosphate (PLP)-dependent condensation of seri

198 Both classes of enzymes catalyze a pyridoxal phosphate (PLP)-dependent condensation of seri

199 nzyme is a unique hemeprotein that catalyzes pyridoxal phosphate (PLP)-dependent condensation of seri

200 Cysteine desulfurases perform pyridoxal phosphate (PLP)-dependent desulfuration of cys

201 t: (i) the bioinformatics analysis reveals a pyridoxal phosphate (PLP)-dependent domain, we termed cy

202 n to be a mechanism-based inactivator of the pyridoxal phosphate (PLP)-dependent enzyme gamma-aminobu

203 xymethyltransferase (cSHMT) is a tetrameric, pyridoxal phosphate (PLP)-dependent enzyme that catalyze

204 lglycine decarboxylase (DGD) is a tetrameric pyridoxal phosphate (PLP)-dependent enzyme that catalyze

205 to the recent report that E. coli BioB is a pyridoxal phosphate (PLP)-dependent enzyme with intrinsi

206 Pyridoxal phosphate (PLP)-dependent enzymes are unrivale

207 Pyridoxal phosphate (PLP)-dependent enzymes can catalyze

208 Pyridoxal phosphate (PLP)-dependent enzymes catalyze man

209 The mechanistic fate of pyridoxal phosphate (PLP)-dependent enzymes diverges aft

210 l therapies for inherited diseases involving pyridoxal phosphate (PLP)-dependent enzymes, including p

211 oacrylate (2AA), an enamine produced by some pyridoxal phosphate (PLP)-dependent enzymes.

212 ase (PanD), the enzyme in M. jannaschii is a pyridoxal phosphate (PLP)-dependent l-aspartate decarbox

213 onic acid (DAPA) synthase (EC 2.6.1.62) is a pyridoxal phosphate (PLP)-dependent transaminase that ca

214 ecies (ROS) detoxification and production of pyridoxal phosphate (PLP).

215 inactivated by the lysine-specific reagent, pyridoxal phosphate (PLP).

216 amin pyrimidine is formed from histidine and pyridoxal phosphate (PLP).

217 l of homocysteine in mammals begins with the pyridoxal phosphate- (PLP-) dependent beta-replacement r

218 The active sites of the homologous pyridoxal phosphate- (PLP-) dependent enzymes 1-aminocyc

219 S-adenosyl-L-methionine (SAM) in the modeled pyridoxal phosphate quinonoid complex with SAM.

220 hildren with intractable seizures respond to pyridoxal phosphate rather than pyridoxine, including a

221 bilizing the protonated pyridine nitrogen of pyridoxal phosphate, reducing the pKa of the internal al

222 and formation of the external aldimine with pyridoxal phosphate required for early steps in SufS cat

223 optimum, Km values, and the requirement for pyridoxal phosphate resemble those of bacterial racemase

224 r pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway.

225 two enzymes of the biosynthetic pathway for pyridoxal phosphate (SerC and PdxA), we have found that

226 kinase (PRK) is inactivated upon exposure to pyridoxal phosphate/sodium borohydride, suggesting a rea

227 ues 1-353) is catalytically active and binds pyridoxal phosphate stoichiometrically establishes that

228 concentrations of glutamate or the cofactor pyridoxal phosphate, suggesting a noncompetitive inhibit

229 te kinetic parameters including the K(d) for pyridoxal phosphate, suggesting that the C-terminal resi

230 was recently found to bind the cofactor PLP (pyridoxal phosphate, the active form of vitamin B(6)).

231 lyzed by this enzyme suggests a role for the pyridoxal phosphate, the role of the heme is uncertain.

232 del containing a pyridoxal-5'-phosphate (P5P/pyridoxal phosphate/the active form of vitamin B(6)) lig

233 lly thought to be due to abnormal binding of pyridoxal phosphate to glutamic acid decarboxylase resul

234 referred to as Pat) that, in the presence of pyridoxal phosphate, transfers the primary amino group o

235 We hypothesized that pyridoxal phosphate (vitamin B6 coenzyme) was de-activat

236 The pyridoxal phosphate was converted to the pyridoxamine fo

237 Fasting plasma concentration of pyridoxal phosphate was inversely associated with myocar

238 5'-phosphate as the product, indicated that pyridoxal phosphate was not directly involved in the rea

239 Plasma levels of pyridoxal phosphate were inversely associated with risk

240 Pyridoxal phosphate, which binds at the intersubunit act