ライフサイエンスコーパス: pyruvate kinase

1 lysis (phosphoglycerate mutase, enolase, and pyruvate kinase).

2 KM2, the gene encoding the glycolytic enzyme pyruvate kinase.

3 n of other ChREBP target genes such as liver pyruvate kinase.

4 nonproliferating tumor cells require active pyruvate kinase.

5 tein kinase, while the tetramer is an active pyruvate kinase.

6 the splice isoforms of the glycolytic enzyme pyruvate kinase.

7 ress exclusively the embryonic M2 isoform of pyruvate kinase.

8 its alpha, beta(1), and beta(2) of plastidic pyruvate kinase.

9 of other known cytoplasmic proteins such as pyruvate kinase.

10 lation of the ChREBP target gene, liver-type pyruvate kinase.

11 he expression of the glucose-responsive gene pyruvate kinase.

12 ndicative for phosphorylation to MANT-ATP by pyruvate kinase.

13 rt of a larger complex of proteins including pyruvate kinase.

14 1,6-bisphosphate (Fru-1,6-BP) in human liver pyruvate kinase.

15 2) is an isoenzyme of the glycolytic enzyme pyruvate kinase.

16 synthase, SREBP1c, chREBP, glucokinase, and pyruvate kinase.

17 opsis genome encodes 14 putative isoforms of pyruvate kinases.

18 y, we demonstrate that the glycolytic enzyme pyruvate kinase 2 (PKM2) is required for sprouting angio

19 ntified the protein as a novel crab allergen pyruvate kinase 2.

20 ctrometry, we report isotopic resolution for pyruvate kinase (232 kDa) and beta-galactosidase (466 kD

21 complexes, such as phosporylase B (194 kDa), pyruvate kinase (232 kDa), and GroEL (801 kDa), to highl

22 permeability, and reduced concentrations of pyruvate kinase, a biomarker of pediatric gastrointestin

23 anslocation, we found that the M2 isoform of pyruvate kinase, a key enzyme in glycolysis, translocate

24 abnormally low levels of the red cell enzyme pyruvate kinase, a known cause of CNSHA.

25 to regulate target genes, such as liver-type pyruvate kinase, acetyl-CoA carboxylase 1, and fatty aci

26 e curves were classified to rapidly identify pyruvate kinase activators and inhibitors with a variety

27 gulates the conversion of protein kinase and pyruvate kinase activities.

28 a(1) subunit causes a reduction in plastidic pyruvate kinase activity and 60% reduction in seed oil c

29 We found increased pyruvate kinase activity and a decreased ratio of reduce

30 r cells leads to a decrease in the levels of pyruvate kinase activity and an increase in the pyruvate

31 C-terminal 51 kDa truncation showed not only pyruvate kinase activity but also activation by aspartat

32 1 expression might be responsible for higher pyruvate kinase activity in db/db mouse retina.

33 Thus, decreased pyruvate kinase activity in PKM2-expressing cells allows

34 with the tyrosine phosphopeptide affects the pyruvate kinase activity of PKM2.

35 e of the growth phenotype with pyruvate, and pyruvate kinase activity of purified recombinant PykM.

36 This resulted not from pyruvate kinase activity per se but rather from the form

37 glycolytic enzyme pyruvate kinase M2 (PKAR; pyruvate kinase activity reporter), which multimerizes a

38 its BC cell survival in a dose-dependent but pyruvate kinase activity-independent manner.

39 hich hinders PKM2 tetramerization and blocks pyruvate kinase activity.

40 termediate phosphoenolpyruvate and decreased pyruvate kinase activity.

41 ported to have chaperone activity: catalase, pyruvate kinase, albumin, lysozyme, alpha-lactalbumin, a

42 We additionally demonstrated that pyruvate kinase also interacts with Kir6.2 subunits.

43 OK257 cells have high lactate dehydrogenase, pyruvate kinase and 3-hydroxyacyl-CoA dehydrogenase acti

44 ranscription of two divergent genes encoding pyruvate kinase and a putative SOS response nuclease, re

45 Two proteins (pyruvate kinase and albumin) were inferred to be related

46 SFEC, pyruvate kinase and aldolase were co-localized by immuno

47 sphofructokinase, lactate dehydrogenase, and pyruvate kinase and analyzed by confocal microscopy.

48 Surprisingly, pyruvate kinase and catalase were at least as effective

49 tasis, is most likely exerted by the enzymes pyruvate kinase and fructose bisphosphatase.

50 e in glycolytic flux, indicated by a rise in pyruvate kinase and lactate dehydrogenase activity, indi

51 ability because of reduced activity of liver pyruvate kinase and malic enzyme, which replenish pyruva

52 cycle flux, which together with increases of pyruvate kinase and phosphoenolpyruvate carboxylase acti

53 hat growth signals reciprocally regulate the pyruvate kinase and protein kinase activities of PKM2 by

54 udy suggests that the conversion between the pyruvate kinase and protein kinase activities of PKM2 ma

55 lated protein(s) regulates the conversion of pyruvate kinase and protein kinase of PKM2 by directly i

56 redictions correctly classify SNP effects in pyruvate kinase and suggest a genetic basis for strain-s

57 etrameric (streptavidin, concanavalin A, and pyruvate kinase), and pentameric (C-reactive protein) co

58 e, glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase, and glucose-6-phosphate isomerase showe

59 Glioma cells expressed both isoforms of pyruvate kinase, and inhibition of either glycolysis or

60 es that uses succinyl-coenzyme A synthetase, pyruvate kinase, and lactate dehydrogenase to couple the

61 example, hexokinase-2, phosphofructokinase, pyruvate kinase, and lactate dehydrogenase), while at th

62 tronic FV vector that expressed EGFP, R-type pyruvate kinase, and MGMTP140K, we were able to increase

63 igh activities of fructokinase, glucokinase, pyruvate kinase, and tricarboxylic acid cycle enzymes, i

64 ins, including Grp58, Grp78, alpha4-actinin, pyruvate kinase, and vimentin.

65 Four isozymes of pyruvate kinase are differentially expressed in human ti

66 coupled with phosphoenol pyruvate (PEP) and pyruvate kinase as an ATP regeneration system.

67 on allosteric regulation in the human liver pyruvate kinase as obtained from full-protein alanine-sc

68 esis required PEP as the phosphate donor and pyruvate kinase as the catalyst.

69 two host glycolytic enzymes, aldolase A and pyruvate kinase, as well as lactate dehydrogenase, are e

70 Cytosolic pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC

71 f the allosteric regulation of rabbit muscle pyruvate kinase by Ala to demonstrate that this effector

72 One of these involved inhibition of pyruvate kinase by citrate, which accumulated and thereb

73 o the allosteric inhibition of rabbit muscle pyruvate kinase by phenylalanine.

74 The assay detected 75 nM ADP produced by the pyruvate kinase-catalyzed phosphorylation of pyruvate wi

75 Therefore, the identified pyruvate kinase catalyzes a crucial step in the conversi

76 Plastidic pyruvate kinase catalyzes a highly regulated, ATP-produc

77 Pyruvate kinase catalyzes the final step in glycolysis a

78 Pyruvate kinase catalyzes the last and rate-limiting ste

79 The addition of this sequence to pyruvate kinase causes the cytoplasmic protein to be loc

80 ied including, LDH (Ra, Ch), G3PDH (Hu, Ch), pyruvate kinase (Ch), Annexin II (Ch), and protein disul

81 activities, decreased lactate dehydrogenase, pyruvate kinase, creatine kinase, and cytochrome c oxida

82 Pyruvate kinase deficiency (PKD) is an autosomal-recessi

83 ose-6-phosphate dehydrogenase deficiency and pyruvate kinase deficiency also confer some degree of re

84 Pyruvate kinase deficiency is caused by mutations in PKL

85 and efficacy of mitapivat in 52 adults with pyruvate kinase deficiency who were not receiving red-ce

86 providing a potential cure for patients with pyruvate kinase deficiency, in vivo selection using foam

87 n the hemoglobin level in 50% of adults with pyruvate kinase deficiency, with a sustained response du

88 nother to cleave near a mutation that causes pyruvate kinase deficiency.

89 canine model of a severe erythroid disease, pyruvate kinase deficiency.

90 pients with chimerism using a mouse model of pyruvate kinase deficiency.

91 hen transplanted into minimally conditioned, pyruvate kinase-deficient recipients (CBA-Pk-1(slc)/Pk-1

92 levels, reducing the drive for production of pyruvate kinase-deficient red blood cells.

93 olic branch point and of its central enzyme, pyruvate kinase (DeltapykF), result in mutants with sign

94 We identified pykM as the only pyruvate kinase-encoding gene based on deficiency in act

95 Paradoxically, decreased pyruvate kinase enzyme activity accompanies the expressi

96 ite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/s

97 disrupted metabolic activity due to altered pyruvate kinase expression and/or alteration in the func

98 nproliferating tumor cells and no detectable pyruvate kinase expression in proliferating cells.

99 Switching pyruvate kinase expression to the M1 (adult) isoform lea

100 erase cleavage while preserving tumor type 2 pyruvate kinase expression.

101 using as a case study the rapid decrease in pyruvate kinase flux in yeast upon glucose removal.

102 The isoform of pyruvate kinase from brain and muscle of mammals (M(1)-P

103 We report X-ray structures of pyruvate kinase from Leishmania mexicana (LmPYK) that ar

104 mparative molecular dynamics analysis of the pyruvate kinase from Leishmania mexicana is presented in

105 The catalytic activities of aldolase and pyruvate kinase functionally modulate K(ATP) channels in

106 or activation of transcription of the L-type pyruvate kinase gene and lipogenic enzyme genes, and (ii

107 (LDHA) expression, together with a switch in pyruvate kinase gene splicing from PKM2 to PKM1, marks t

108 sequestrin), glucose metabolism (hexokinase, pyruvate kinase, Glut4), oncogenesis (TGFbeta1, cathepsi

109 hydrogenase, myosin light chain, aldolase A, pyruvate kinase, glycogen phosphorylase, actinin, gamma-

110 ntial of this approach utilizing human liver pyruvate kinase (hLPYK) as a model.

111 glucose transporter-4, hexokinase-2, muscle-pyruvate kinase, hormone-sensitive lipase, and uncouplin

112 We found that pyruvate kinase II (PyrKII) is essential for organelle m

113 phosphoenolpyruvate (PEP), the substrate for pyruvate kinase in cells, can act as a phosphate donor i

114 r, our study identifies an essential role of pyruvate kinase in preventing metabolic block during car

115 .2 protein similarly interact with GAPDH and pyruvate kinase in rat heart membrane fractions and that

116 oral, small-molecule allosteric activator of pyruvate kinase in red cells.

117 We report that knockdown of pyruvate kinase in tumor cells leads to a decrease in th

118 es expression of the embryonic M2 isozyme of pyruvate kinase, in contrast to the M1 isozyme normally

119 APDH, triosephosphate isomerase, and M2-type pyruvate kinase increased approximately two- to threefol

120 is demonstrated by experiments in which the pyruvate kinase inhibitor, phenylalanine, is added to ce

121 Thus, while yeast pyruvate kinase is covalently modified in response to gl

122 in a splice isoform of the glycolytic enzyme pyruvate kinase is necessary for the shift in cellular m

123 as glycolysis, where pathway outflow through pyruvate kinase is regulated by the concentration of a k

124 Pyruvate kinase is the only regulatory step of the commo

125 d the glycolytic activity of PKM2, the major pyruvate kinase isoenzyme known to regulate cellular glu

126 y a SiLAD proteomics analysis, we identified pyruvate kinase isoenzyme M2 (PKM2), a critical regulato

127 We find that a deficiency in the M2 pyruvate kinase isoform (PKM2) reduces the levels of met

128 , this is partly achieved through control of pyruvate kinase isoform expression.

129 Pyruvate kinase isoform M2 (PKM2) is a glycolysis enzyme

130 Pyruvate kinase isoform M2 (PKM2) is an enzyme-catalyzin

131 It is long known that pyruvate kinase isoform M2 (PKM2) is released into the c

132 Here, we demonstrated that the nuclear pyruvate kinase isoform M2 (PKM2) levels were positively

133 Pyruvate kinase isoform M2 (PKM2) plays an important rol

134 s that promote the metabolic activity of the pyruvate kinase isoform PKM2, such as TEPP-46 and DASA-5

135 The embryonic pyruvate kinase isoform, PKM2, is almost universally re-

136 The influence of pyruvate kinase isoforms on tumor cells has been extensi

137 The pyruvate kinase isoforms PKM1 and PKM2 are alternatively

138 ed protein product (UNP) similar to enolase, pyruvate kinase, isoforms of creatine kinase, aldolase A

139 The main pyruvate kinase isozyme (Cdc19) is phosphorylated in res

140 ological activators of a tumor cell specific pyruvate kinase isozyme (PKM2) may be an approach for al

141 Human pyruvate kinase isozyme M2 (hPKM2) is expressed in early

142 glycolytic enzymes alpha-enolase (ENO1) and pyruvate kinase isozyme M2 (PKM2), were assessed for the

143 ng T-complex polypeptide 1 subunit zeta, and pyruvate kinase isozyme.

144 The M1 and M2 pyruvate-kinase isozymes are expressed from a single gen

145 Although pyruvate kinase knockdown results in modest impairment o

146 is from both glucose and glutamine following pyruvate kinase knockdown.

147 ate-responsive element-binding protein-beta, pyruvate kinase L, SCD-1, and DGAT1, key transcriptional

148 h glucose and reduced the activity of L-type pyruvate kinase (L-PK) and TxNIP promoters, two well-cha

149 ciprocally regulate expression of the L-type pyruvate kinase (L-PK) gene by controlling the formation

150 Glucose-mediated activation of the L-type pyruvate kinase (L-PK) gene is repressed by cAMP, making

151 glucose-mediated induction of hepatic L-type pyruvate kinase (L-PK) gene transcription.

152 The initial 26 amino acids of human liver pyruvate kinase (L-PYK) are not present/observed in the

153 ize the regulatory properties of human liver pyruvate kinase (L-PYK), we have noted that the affinity

154 y metabolomics revealed that inactivation of pyruvate kinase leads to accumulation of phosphoenolpyru

155 We quantified gene copy numbers of the pyruvate kinase, liver, and red blood cell (PKLR) gene a

156 by its ability to bind the ChRE of the liver pyruvate kinase (LPK) gene.

157 splicing in VAChT-deficient mice, including pyruvate kinase M, a key enzyme involved in lactate meta

158 with allosteric regulation in rabbit muscle pyruvate kinase (M(1)-PYK).

159 Here, we assessed whether disrupting pyruvate kinase-M (Pkm), an enzyme that acts in the term

160 Although normal cells express the pyruvate kinase M1 isoform (PKM1), tumor cells predomina

161 human cancer cell lines and replace it with pyruvate kinase M1.

162 ET biosensors based on the glycolytic enzyme pyruvate kinase M2 (PKAR; pyruvate kinase activity repor

163 romote dimerization of the glycolytic enzyme pyruvate kinase M2 (PKM2) and enable its nuclear translo

164 Here we identify pyruvate kinase M2 (PKM2) as a novel PTP1B substrate in

165 In particular, pyruvate kinase M2 (PKM2) expression and activity were u

166 f CLL cells, indicated by down-regulation of pyruvate kinase M2 (PKM2) expression and activity, decre

167 , we identified exosome-mediated transfer of pyruvate kinase M2 (PKM2) from PCa cells into bone marro

168 The role of pyruvate kinase M2 (PKM2) in cell proliferation is contr

169 The former, in turn, was mediated by pyruvate kinase M2 (PKM2) interaction with soluble adeny

170 Pyruvate kinase M2 (PKM2) is a key enzyme for glycolysis

171 Pyruvate kinase M2 (PKM2) is a metabolic enzyme that pla

172 Pyruvate kinase M2 (PKM2) is also induced and interacts

173 Tumor-specific pyruvate kinase M2 (PKM2) is essential for the Warburg e

174 Pyruvate kinase M2 (PKM2) is expressed at high levels du

175 Tumor-specific pyruvate kinase M2 (PKM2) is instrumental in both aerobi

176 The embryonic pyruvate kinase M2 (PKM2) isoform is highly expressed in

177 is mediated by inhibitory S-nitrosylation of pyruvate kinase M2 (PKM2) through a novel locus of regul

178 S caused inhibition of the glycolytic enzyme pyruvate kinase M2 (PKM2) through oxidation of Cys(358).

179 The tumor form of pyruvate kinase M2 (PKM2) undergoes tyrosine phosphoryla

180 Moreover, levels of pyruvate kinase M2 (PKM2) were increased in this model a

181 Recent data indicate that pyruvate kinase M2 (PKM2), a glycolytic enzyme for Warbu

182 Here, we show that the pyruvate kinase M2 (PKM2), a glycolytic enzyme required

183 ship of TG2 to a terminal glycolytic enzyme, pyruvate kinase M2 (PKM2), and found that PKM2 regulates

184 key glycolytic proteins, including enolase, pyruvate kinase M2 (PKM2), lactate dehydrogenase and mon

185 tic intrabody, intrabody 5 (IB5), recognized pyruvate kinase M2 (PKM2), which is expressed in cancer

186 ide evidence to support a novel role for the pyruvate kinase M2 (PKM2)-mediated Warburg effect, namel

187 ic glycolysis and tumor growth by inhibiting pyruvate kinase M2 (PKM2).

188 ypes of human tumor cells have overexpressed pyruvate kinase M2 (PKM2).

189 ng, as a model system, the glycolytic enzyme pyruvate kinase M2 (PKM2).

190 ylation at Y59, which interacts with nuclear pyruvate kinase M2 (PKM2).

191 Here we use short hairpin RNA to knockdown pyruvate kinase M2 expression in human cancer cell lines

192 In addition, loss of the glycolytic enzyme pyruvate kinase M2 impairs trabeculation.

193 The pyruvate kinase M2 isoform (PKM2) is expressed in cancer

194 Very recently, we found that pyruvate kinase M2 isoform (PKM2) regulates visual funct

195 noma (HCC) through nuclear relocalization of pyruvate kinase M2 isoform (PKM2), a key regulator of th

196 oma (HCC) by maintaining low activity of the pyruvate kinase M2 isoform (PKM2), a key regulator of th

197 ncluding many metabolic proteins such as the pyruvate kinase M2 isoform (PKM2).

198 Tumor pyruvate kinase M2 isoform (tM2-PK), which is an isoform

199 s and remarkable downregulation of c-Myc and pyruvate kinase M2 isoform, the key glycolytic enzyme tr

200 IRT6 binds to and deacetylates nuclear PKM2 (pyruvate kinase M2) at the lysine 433 residue.

201 AR (androgen receptor) and PKM2 (pyruvate kinase M2) have key roles in these processes.

202 nometabolism, with increased cytosolic PKM2 (pyruvate kinase M2), phosphorylated PKM2, HIF-1alpha (hy

203 a Warburg effect, including cytosolic PKM2 (pyruvate kinase M2), phosphorylated PKM2, succinate, HIF

204 Importantly, pyruvate kinase M2, a fetal anabolic enzyme implicated i

205 ark cancer genes, including hexokinase 2 and pyruvate kinase M2.

206 lycolytic pathway, 6-phosphofructokinase and pyruvate kinase M2.

207 CC cells by targeting hexokinase-2 (Hk2) and pyruvate kinase-M2 (Pkm2).

208 acetylation and lysosomal degradation of the pyruvate kinase-M2 isoform (PKM2).

209 We show that the M2 isoform of pyruvate kinase (M2PYK) exists in equilibrium between mo

210 e for peptides from novel variants of muscle pyruvate kinase, malate dehydrogenase 1, glyceraldehyde-

211 se-transporter-1 mRNA, and of Hexokinase and Pyruvate-Kinase mRNAs, key regulators of glycolysis.

212 xpression to control alternative splicing of pyruvate kinase muscle (PKM) isoforms 1 and 2, resulting

213 Moreover, we show that the metabolic enzyme, pyruvate kinase muscle (PKM), interacts with sub-pools o

214 y in nucleus pulposus (NP) cells through the pyruvate kinase muscle (PKM)-2-Jumonji domain--containin

215 The glycolytic enzyme Pyruvate Kinase Muscle 2 (PKM2) has described roles in r

216 Pkm2 (Pyruvate kinase muscle isoenzyme 2) is an isoenzyme of t

217 al proximity of Tnfalpha alleles depended on pyruvate kinase muscle isoform 2 (PKM2) and T-helper-ind

218 lar to cancer cells, photoreceptors maintain pyruvate kinase muscle isoform 2 (PKM2) expression, whic

219 Pyruvate kinase muscle isoform 2 (PKM2) is a key glycoly

220 Pyruvate kinase muscle isoform 2 (PKM2) is a key glycoly

221 otein), resulting in alternative splicing of pyruvate kinase muscle isoforms 1 and 2 (PKM1 and 2) and

222 JMJD5 interacts directly with pyruvate kinase muscle isozyme (PKM)2 to modulate metabo

223 Pyruvate kinase muscle isozyme 2 (PKM2) is a key regulat

224 ic reprogramming and IFN-gamma secretion via pyruvate kinase muscle isozyme 2 (PKM2) to accelerate at

225 n of hypoxia-inducible factor-1A and reduced pyruvate kinase muscle isozyme 2 activity, both key regu

226 vesicles regulate B-cell IgG production via pyruvate kinase muscle isozyme 2.

227 ncreased expression of the glycolytic enzyme pyruvate kinase muscle isozyme M2 and after KIR cross-li

228 utase 2, glycogen phosphorylase muscle form, pyruvate kinase muscle isozyme, beta-enolase and triosep

229 spots identified as glycogen phosphorylase, pyruvate kinase muscle isozyme, isoforms of creatine kin

230 glucose-6-phosphate dehyrogenase (G6PD) and pyruvate kinase muscle type 2 (PKM2) that facilitates vi

231 divided by CCS fwhm) of ~60 is obtained for pyruvate kinase (MW ~ 233 kDa); however, ion mobility re

232 NDP kinase to suppress anaerobic growth in a pyruvate kinase-negative E. coli mutant.

233 EGFR and that of a green fluorescent protein-pyruvate kinase-NLS reporter protein.

234 aliana) mutant (pkp1) deficient in plastidic pyruvate kinase (PK(p)) and unable to accumulate storage

235 While in both wild types plastidic pyruvate kinase (PK(p)) provides most of the pyruvate fo

236 Muller glia are deficient for pyruvate kinase (PK) and for aspartate/glutamate carrier

237 -opts the cellular glycolytic ATP-generating pyruvate kinase (PK) directly into the viral replicase c

238 Pyruvate kinase (PK) exists in M1 (PKM1) and M2 (PKM2) i

239 shows the critical role of glycolytic enzyme pyruvate kinase (PK) in directing metabolism of prolifer

240 Pyruvate kinase (PK) is the main "pacemaker" of the EDP,

241 carbon source but the lack of a functioning pyruvate kinase (PK) means that carbohydrates cannot be

242 of the low-activity (dimeric) M2 isoform of pyruvate kinase (PK) over its constitutively active spli

243 In mammals, pyruvate kinase (PK) plays a key role in regulating the

244 sing excess CTP instead of ATP as substrate, pyruvate kinase (PK), and firefly luciferase) to generat

245 duct generates the PKM1 and PKM2 isoforms of pyruvate kinase (PK), and PKM2 expression is closely lin

246 GAPDH, aldolase, phosphofructokinase (PFK), pyruvate kinase (PK), lactate dehydrogenase (LDH), carbo

247 Luo et al. now identify pyruvate kinase (PK)-M2 as an intriguing new interacting

248 e, whereas another could involve reversal of pyruvate kinase (PK).

249 reen, we identified liver and red blood cell pyruvate kinase (PKLR) as a driver of metastatic liver c

250 ted Sites (BORIS) at the alternative exon of Pyruvate Kinase (PKM) is associated with cancer-specific

251 mely, the rate-limiting enzyme of glycolysis pyruvate kinase (PKM), which plays a critical role in ca

252 d decrease in the expression and activity of pyruvate kinase PKM2, a glycolytic enzyme that indirectl

253 We find that SHMT2 activity limits that of pyruvate kinase (PKM2) and reduces oxygen consumption, e

254 In this study, we identify the M2 isoform of pyruvate kinase (PKM2) as a critical target of the sirtu

255 phorylation of the tumor-specific isoform of pyruvate kinase (PKM2) at Y105, resulting in decreased e

256 Upregulation of the embryonic M2 isoform of pyruvate kinase (PKM2) emerges as a critical player in t

257 active tetramer and inactive dimer forms of pyruvate kinase (PKM2) in cancer cells, similar to the t

258 The M2 isoform of pyruvate kinase (PKM2) promotes the metabolism of glucos

259 e we report that the embryonic M2 isoform of pyruvate kinase (PKM2), a key enzyme contributing to the

260 e M2 isoform of the tightly regulated enzyme pyruvate kinase (PKM2), which controls glycolytic flux,

261 dominance of expression of the M2 isoform of pyruvate kinase (PKM2).

262 tively express the less active M2 isoform of pyruvate kinase (PKM2).

263 a double mutant in two isoforms of plastidic pyruvate kinase (pkpbeta(1)pkpalpha; At5g52920 and At3g2

264 f interleukin-3, suggesting that the nuclear pyruvate kinase plays an important role in cell prolifer

265 axoneme, whereas phosphoglycerate mutase and pyruvate kinase primarily reside in the detergent-solubl

266 Overexpression of the M2 isoform of pyruvate kinase protein fused with a nuclear localizatio

267 tation and were associated with the red-cell pyruvate kinase protein level at baseline.

268 ously established consequences of abolishing pyruvate kinase (Pyk) activity in Escherichia coli durin

269 Pyruvate kinase (PYK) is an essential glycolytic enzyme

270 Pyruvate kinase (PYK) is an essential glycolytic enzyme

271 different conformers of the same species of pyruvate kinase (PYK).

272 olysis and theMtbgenome harbors one putative pyruvate kinase (pykA, Rv1617).

273 on aldehyde-alcohol dehydrogenase (AdhE) and pyruvate kinase (PykF) enzymes, previously not known to

274 t allosteric regulation of the activities of pyruvate kinase (PykF, but not PykA), phosphofructokinas

275 eptide antibodies established that cytosolic pyruvate kinase (PyrKinc) is phosphorylated at both site

276 operation of the phosphoglycerate kinase and pyruvate kinase reactions to enzymatically generate ATP.

277 GAPDH, aldolase, lactate dehydrogenase, and pyruvate kinase revealed not only the anticipated bindin

278 ibility of this approach using rabbit muscle pyruvate kinase (rM1-PK) which catalyzes the conversion

279 NA ends, 2-phospho-L-lactate is a product of pyruvate kinase side reaction, and both potently inhibit

280 uvate kinase activity and an increase in the pyruvate kinase substrate phosphoenolpyruvate.

281 s is demonstrated by the ability of GAPDH or pyruvate kinase substrates to directly block the K(ATP)

282 l C-terminal truncation to generate a 51 kDa pyruvate kinase subunit which might have altered regulat

283 dynamic movement of the holo form traps the pyruvate kinase tetramer in its enzymatically active sta

284 Here we show thatpykAencodes an active pyruvate kinase that is allosterically activated by gluc

285 These fluxes changed with plastidic pyruvate kinase to maintain a supply of pyruvate for ami

286 Fusions of pyruvate kinase to the lentiviral integrases did not rev

287 ntitative HTS (qHTS), tested with the enzyme pyruvate kinase, to generate concentration-response curv

288 d activation of fructose-1,6-bisphosphate on pyruvate kinase translate flux information into the conc

289 are proposed to explain how Escherichia coli pyruvate kinase type 1 is allosterically regulated: the

290 our cell exosomes secretion is controlled by pyruvate kinase type M2 (PKM2), which is upregulated and

291 versible aggregation of the metabolic enzyme pyruvate kinase under environmental stress and propose a

292 vely, fructose 1,6-bisphosphate aldolase and pyruvate kinase, under the same conditions.

293 d nuclear translocation of the M2 isoform of pyruvate kinase was dependent on the activation of Jak2.

294 hosphate isomerase was up-regulated, whereas pyruvate kinase was down-regulated.

295 In addition, the glycolytic enzyme pyruvate kinase was found to be phosphorylated by PrkD o

296 The C domain of the M2 isoform of pyruvate kinase was sufficient for interleukin-3-induced

297 peroxide dismutase and the glycolytic enzyme pyruvate kinase were deficient in an mntH strain grown u

298 r neurons express AGC1 and the M2 isoform of pyruvate kinase, which is commonly associated with aerob

299 monovalent cation with wild type (WT) yeast pyruvate kinase (YPK) and with the T298S, T298C, and T29

300 The active site T298 residue of yeast pyruvate kinase (YPK), located in a position to serve po