ライフサイエンスコーパス: quantitation

1 cal sensitivity, and limits of detection and quantitation.

2 it of MS, the current gold standard of cGAMP quantitation.

3 pecies that can lead to inaccurate stability quantitation.

4 tigated by shotgun proteomics and label-free quantitation.

5 ediately spiked and for external calibration quantitation.

6 alysis, and isobaric labeling and label-free quantitation.

7 destructuration using imaging and label-free quantitation.

8 g, and monosaccharide and glycosidic linkage quantitation.

9 t enable robust use of FAIMS for multiplexed quantitation.

10 ed internal standard method was used for the quantitation.

11 database search, statistical validation, and quantitation.

12 e good separation of the vaccine product for quantitation.

13 the accuracy and dynamic range of multiplex quantitation.

14 ating treatment were at or near the limit of quantitation.

15 easily synthesized internal standard for MS quantitation.

16 Concentration determination), were used for quantitation.

17 , as well as the reference material used for quantitation.

18 le analysis and lack the ability of absolute quantitation.

19 th internal standards was chosen for analyte quantitation.

20 ccess software Skyline is used for automated quantitation.

21 ssay (Simoa; Quanterix) with lower limits of quantitation.

22 mpers accurate metabolite identification and quantitation.

23 t from metabolites of interest, was used for quantitation.

24 niformity, count rate performance, and image quantitation.

25 nfocal microscopy, and intracellular calcium quantitation.

26 We consider the optimum height for quantitation.

27 3-3%, validating our CMS method for absolute quantitation.

28 challenge for this targeted MS/MS-based PTM quantitation.

29 tibody light chain are conveniently used for quantitation.

30 eoform identification, characterization, and quantitation.

31 maximizing phosphopeptide identification and quantitation.

32 nal and thus extend the dynamic range of the quantitation.

33 ts of detection (0.05-10 ng/g) and limits of quantitation (0.15-30 ng/g) for the analytes tested.

34 sed biosensor capable of ultra-low (limit of quantitation: 0.1 pM) detection.

35 zed by relatively low limit of detection and quantitation (2.0 and 4.9 mg L(-1), respectively), good

36 rmance of MASQ (multiplex accurate sensitive quantitation), a method with an error rate below 10-6.

37 dditional manual correction greatly enhances quantitation accuracy.

38 led phosphate combined with isotope dilution quantitation allows measurement of the phosphate levels

39 This study describes the use of amino acid quantitation and amino-acid-specific isotope ratio analy

40 We assessed the association between quantitation and function of CMV-specific CD8+ T cells a

41 reference for ligand screening and affinity quantitation and further revealed the advantages of SEC-

42 ith mass spectrometry (MS) provides relative quantitation and has the ability to perform automated gl

43 ance (MR) probe that enables the ratiometric quantitation and imaging of pH through chemical exchange

44 omes into recipient circulation and that the quantitation and profiling of donor intra-exosomal cargo

45 nd medicine, which in turn demand increasing quantitation and reproducibility.

46 Orbitrap allowed for protein ligand affinity quantitation and resolved individual compounds close in

47 utility of our protocol and Ab panels in the quantitation and spatial distribution of immune cells.

48 h allows drastic reduction of false positive quantitations and identifications even from phosphopepti

49 es across assays, MS features as a basis for quantitation, and evidence to support the reporting of i

50 to limited tumor (18)F-FDG uptake, erroneous quantitation, and significant image artifacts.

51 performs most popular MS/MS-based label-free quantitation approaches because of significantly higher

52 Protein and peptide identification and quantitation are essential tasks in proteomics research

53 monitoring (MRM) mode, with a lower limit of quantitation at 1.00 nM.

54 hy-tandem mass spectrometry, with a limit of quantitation at 2.2 lesions per 10(8) nucleotides (nts,

55 utomatically performs large-scale proteoform quantitation based on interspectrum abundance in top-dow

56 RAQ (isobaric tags for relative and absolute quantitation)-based quantitative proteomics revealed tha

57 rometric (CMS) approach for absolute protein quantitation, based on the electrochemical oxidation of

58 from isobaric tags for relative and absolute quantitation-based quantitative proteomic experiments wi

59 anned doping substances, achieving limits of quantitation below the minimum required performance limi

60 and SPME-LC-MS/MS methods achieved limits of quantitation below their minimum regulatory limits.

61 isotopically labeled internal standard, and quantitation by LC-MS/MS analysis.

62 and nicarbazin in egg were optimized for its quantitation by liquid chromatography.

63 However, robust and accurate quantitation by MALDI-MSI still remains a challenge.

64 Popular methods for absolute protein quantitation by mass spectrometry (MS) involve the diges

65 en elements were observed above the limit of quantitation by using inductively coupled plasma mass sp

66 ytical problem, metal ion discrimination and quantitation, by constructing a sensor array that provid

67 at the multilabel approach provides superior quantitation compared to a single-label approach, in cas

68 ides were selected as surrogate peptides for quantitation, considering the oxidizable nature of tyros

69 typically recommended for single-cell sample quantitation, consistently overestimate viability.

70 Recent quantitation developments using cardiovascular magnetic

71 The sensor provides PfLDH quantitation down to 2.47 ng mL(-1) in spiked samples an

72 The source of gluten can influence gluten quantitation due to variability in protein profile of gr

73 es accurate phosphate results and eliminates quantitation errors caused by phosphate adsorption.

74 been the gold standard for sensitive protein quantitation for decades.

75 Changes in PET quantitation for low-dose images were assessed using vol

76 c peptide assignment, protein inference, and quantitation for multi-species proteomics experiments.

77 endent axial aberrations that could confound quantitation, highlighting the need for the "aberration

78 The dynamic range and accuracy of quantitation, however, can be limited by coisolation of

79 A (ctDNA) and microRNA (miRNA) detection and quantitation in body fluids.

80 bel-free approach for inorganic nanoparticle quantitation in cells.

81 lides to improve the accuracy of Ki-67 index quantitation in GI-NETs: (1) Synaptophysin-KI-Estimator

82 amic range offered by NAPA promises improved quantitation in imaging.

83 uous measure which allows for precise allele quantitation in loci where the RNA alleles do not scale

84 2-AP was below the limits of quantitation in non-fragrant varieties; however, gas chr

85 HS-SPME-GC-MS method was optimized for their quantitation in numerous must and wine samples, providin

86 which eliminates the requirement of absolute quantitation in traditional MFA and thus enables compara

87 Due to the high specificity, quantitation in various biological matrixes was achieved

88 xcretion, making difficult its detection and quantitation in vivo.

89 Hepatitis C virus (HCV) RNA quantitation is the primary method by which active HCV i

90 ontaining peptides; additionally, label-free quantitation is used to ascertain catalytic efficiency v

91 sing isobaric tags for relative and absolute quantitation (iTRAQ) 4-plex, >4,500 proteins are detecte

92 the isobaric tags for relative and absolute quantitation (iTRAQ) based comparative proteomics.

93 Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) proteomic analysis by tandem mass s

94 sing isobaric tags for relative and absolute quantitation (iTRAQ) proteomics.

95 gram, Isobaric tag for relative and absolute quantitation (iTRAQ) with Liquid chromatography-mass spe

96 sing isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis.

97 such as linearity, accuracy, precision, and quantitation limit.

98 les with excellent precision and recovery at quantitation limits of 0.025 mug/L of MC.

99 is ITEX method provided 2-3 magnitudes lower quantitation limits than the headspace dynamic ITEX meth

100 ental limit of quantitation, method limit of quantitation, linearity, accuracy, and precision.

101 Limits of Detection and Quantitation (LOD and LOQ) were estimated for all the st

102 00 ug L(-1), and the limits of detection and quantitation (LOD and LOQ) were found to be 8.7 and 29.0

103 ection (LOD) (3SD(blank)/Slope) and limit of quantitation (LOQ) (10SD(blank)/Slope) were found to be

104 determine the limits of detection (LOD) and quantitation (LOQ) for each compound with both methods.

105 detection (LOD) of 2.5 pg/mL and a limit of quantitation (LOQ) of 3.6 pg/mL.

106 In the targeted dMRM mode, the limit of quantitation (LOQ) of nine standard glycoproteins reache

107 The limit of detection (LOD) and limit of quantitation (LOQ) were achieved as 0.019 and 0.062 ng m

108 inearity, limit of detection (LOD), limit of quantitation (LOQ), matrix effects, robustness, uncertai

109 The limits of quantitation (LOQs) obtained were in the low-ppb range i

110 all the studied compounds, and the limits of quantitation (LOQs) were within the range of 0.1 to 10 n

111 CP-MS), which we term metal-assisted protein quantitation (MAPq).

112 rom a minimal sample cohort using label-free quantitation mass spectrometry.

113 re, the tests yielded satisfactory limits of quantitation (median, approximately 0.5 ng mL(-1)), line

114 Conclusion: This noninvasive in vivo quantitation method can potentially improve diagnosis, p

115 iate internal standard is a well-established quantitation method for assigning purity to organic mole

116 analysis using an isobaric tag for relative quantitation method.

117 idated in terms of the instrumental limit of quantitation, method limit of quantitation, linearity, a

118 distinguishable by other commonly used mtDNA quantitation methods.

119 argeted mass spectrometry assays for protein quantitation monitor peptide surrogates, which are easil

120 By enabling fast and free-breathing quantitation, MRF has the potential to add value during

121 sphopeptide identity, site-localization, and quantitation must often be gleaned from a single peptide

122 SUV(mean), CAA, and %ID measured by absolute quantitation of (99m)Tc-pyrophosphate were moderately co

123 of 0.11%, a limit of detection and limit of quantitation of 0.15% for unrelated donors, and limit of

124 of 0.23%, a limit of detection and limit of quantitation of 0.29% for related donors.

125 samples demonstrates reproducible label free quantitation of 1 mug protein homogenates.

126 matics pipeline that allows the simultaneous quantitation of 10 transplant-related double-stranded DN

127 for rapid and high-throughput screening and quantitation of 105 veterinary drugs in homogenized bovi

128 MS method for the simultaneous detection and quantitation of 14 acylated lysine species in biological

129 -CBS technology was used for the concomitant quantitation of 18 compounds (mixture of anabolics, beta

130 romatography and mass spectrometry for rapid quantitation of 25OHD in human serum.

131 mit of detection of 0.5 pg/mL and a limit of quantitation of 5 pg/mL with virtually no background sig

132 epetitive HPLC fractionation resulted in the quantitation of 6 additional acylation structures includ

133 chniques have enabled the identification and quantitation of a defined subset of proteins/peptides at

134 med the benefits of qH{(13)C}NMR through the quantitation of a diverse set of compounds, namely, smal

135 ensitive diagnostic assay for the ultratrace quantitation of a phosphoryl fluoride nerve agent surrog

136 monstrated in this work with the examples of quantitation of a protein biomarker in human and monkey

137 analytical platforms enabling detection and quantitation of adulteration.

138 Hence, accurate quantitation of alpha-tubulin acetylation is required in

139 mple, antibody-free proteomics assay enables quantitation of alpha-tubulin acetylation, and is applic

140 s assay to characterize and perform relative quantitation of antibody species from 248 samples of 62

141 tial for broader application to the absolute quantitation of any endogenous protein or protein-based

142 advantage was the capability of simultaneous quantitation of ASO metabolites.

143 S was considered an improved alternative for quantitation of ASOs and metabolites in plasma and tissu

144 olymerase chain reaction of genomic DNA, and quantitation of Bmp6 messenger RNA expression from isola

145 providing the first example of a ratiometric quantitation of Ca(2+) concentration using PARACEST.

146 s and developed a novel assay for the direct quantitation of cell-associated Hoechst.

147 biology is the automated identification and quantitation of cells in complex multilayered tissues.

148 ibe here a new method for identification and quantitation of CoA and acetyl-CoA ex vivo in tissue.

149 This enables improved quantitation of cross-linked peptide levels from proteom

150 nstrated in this work with an example of the quantitation of daclatasvir in human plasma extracted wi

151 e efficient isolation, characterization, and quantitation of drug substances in the development of ne

152 cell lung cancer (NSCLC), but protein level quantitation of drug targets presents a critical problem

153 in rapid diagnostics for identification and quantitation of E. coli and other pathogens, also regard

154 A convenient approach is proposed for the quantitation of elemental cofactors in proteins and the

155 The capability of NAPA-MS for targeted quantitation of endogenous metabolites in urine via addi

156 Analytical methods for the detection and quantitation of esketamine were developed and validated

157 It relied on the quantitation of extravasated bovine serum albumin conjug

158 source software called QuantiFish for rapid quantitation of fluorescent foci in zebrafish larvae, to

159 The tolerance to matrix is demonstrated by quantitation of fluoride in infant formula, yielding rec

160 oteome and validate a methodology for global quantitation of folding thermodynamics.

161 rd Method Performance Requirements), for the quantitation of four bioactive forms of vitamin B12 (ade

162 However, the systematic quantitation of glycoproteins has remained largely unexp

163 However, direct quantitation of glycoproteins in serum remains technical

164 mass spectrometry (LC-MS/MS) method for the quantitation of glycosidic linkages derived from disacch

165 or identification, confirmation and relative quantitation of GPLs in vegetable oils.

166 ibody depletion, which challenges the direct quantitation of HCPs.

167 evaluate the purity of our plasma sample by quantitation of hemoglobin and report hemolysis as eithe

168 ive MS-based technique in both detection and quantitation of homodimer impurities in bsAb samples.

169 mmSEC-MS) for highly sensitive detection and quantitation of homodimer impurities in bsAb samples.

170 Detection and quantitation of homodimer impurities in therapeutic bisp

171 evaluated as a potential PET tracer for the quantitation of human islets transplanted to the liver.

172 phenotypic analysis of cellular immunity and quantitation of humoral immunity.

173 Quantitation of ICAM-1 protein expression on cells and v

174 learning (ML) to perform classification and quantitation of images of nuclei from human blood neutro

175 lly, we show the use of VTEA for large-scale quantitation of immune cells in entire human kidney biop

176 Quantitation of immune checkpoint interaction by direct

177 The assay was evaluated for quantitation of interleukin-6 (IL-6), a cytokine biomark

178 than PCR amplification influences the biased quantitation of intermediately and lowly expressed micro

179 rast against liver tissue, enabling accurate quantitation of islet mass.

180 on double bond position(s) and (ii) relative quantitation of isomeric fatty acyl substituents.

181 Quantitation of lipid structure isomers at multiple leve

182 st (ca. 1 min) and enables the profiling and quantitation of lipids in small-volume biofluid samples.

183 Here, we evaluate the detection and quantitation of low-level viremia using the following tw

184 target components with direct impact in the quantitation of metabolites and potential genotoxic impu

185 in vivo systems, we show successful relative quantitation of MHC-I ligands using TMT-based multiplexi

186 Direct quantitation of microbial necromass in soil is very chal

187 utes the identification and highly sensitive quantitation of microcystins (MCs) as model targets.

188 of MK-4 as an internal standard enabled the quantitation of microgram level of vitamin K compounds i

189 techniques for the fast, specific and simple quantitation of microRNAs are becoming crucial enablers

190 multaneously while retaining high efficiency quantitation of microRNAs in low input samples from tiss

191 mic range that enables real-time subcellular quantitation of mitochondrial pyruvate transport, concen

192 ectrometry for multiplexed site-specific PTM quantitation of monoclonal antibodies to overcome this l

193 n, an automated method for the screening and quantitation of more than 150 pesticides from apple juic

194 e demonstrate the simultaneous detection and quantitation of multiple biothiols, including up to thre

195 ) workflow is proposed for the screening and quantitation of multiple substances (i.e., drugs of abus

196 coefficient of variation (CV)) < 25%, n = 6) quantitation of N-glycans within 2 orders of magnitude.

197 N; reproducibility, resolution, and relative quantitation of N-glycome using ultra-performance liquid

198 To achieve the quantitation of N-glycome with high accuracy and sensiti

199 atforms are promising as they allow absolute quantitation of NA in individuals which is essential to

200 erally correlated with neutralization titer, quantitation of neutralization potency revealed that hig

201 ing internal standard procedure for accurate quantitation of Nisin A in cheese becomes available.

202 method for in-depth structural analysis and quantitation of O-glycans derived from human cell membra

203 We find that sequencing caveats affect quantitation of only a subset of human tRNA genes.

204 pression along the corticomedullary axis and quantitation of osmotically regulated genes, allowing th

205 chloroform (CDCl(3)), demonstrating accurate quantitation of PBAT by q-(1)H NMR.

206 Using this method, we demonstrate the quantitation of perfluorinated surfactants in water, a g

207 methodology detailing the mass spectrometric quantitation of PF-05212384 dosed as a targeted polymeri

208 etry (2D-IC-ESI-MS) allowed isotope dilution quantitation of phosphate with simultaneous analysis of

209 d a quick, accurate and simple detection and quantitation of PIK3CA mutations in two breast cancer co

210 t enable specific, rapid, and ultrasensitive quantitation of protein activity in native samples would

211 the first immunoassay platform for absolute quantitation of protein biomarkers in FFPE samples to me

212 Developing immunoassay for absolute quantitation of protein biomarkers in Formalin Fixed Par

213 Overall, QUAD allows the first global quantitation of protein stability rates in tissues, whic

214 traditional label-free approach for relative quantitation of PTMs requires a great amount of instrume

215 This approach enables the simultaneous quantitation of quality attributes (e.g., PTMs) for mult

216 Accurate quantitation of rapid protein synthesis changes can prov

217 urther engineered to bind xenon, enables the quantitation of ribose over a wide concentration range (

218 nded with an anti-RRV antibody response, and quantitation of RRV DNA in peripheral blood mononuclear

219 otopic abundance of IA%) can be used for the quantitation of samples having concentrations beyond the

220 (isotopic abundance of 100%) is used for the quantitation of samples having concentrations within the

221 The bead-based serological assay for quantitation of SARS-CoV-2-specific antibodies proved to

222 lied for the analysis of natural waters with quantitation of SeCN(-) in the low ng/g region.

223 A method for the simultaneous quantitation of seven markers in guarana by HPLC-PDA was

224 an established LC-MS/MS-based method for the quantitation of several imidazole alkaloids in tomato pr

225 Thus, our findings show quantitation of SHH expression by CAM can provide a tool

226 in the characterization is the detection and quantitation of siRNA from biological samples.

227 ) are routinely screened for in soils, where quantitation of structural isomers is critical due to va

228 exact blood volume in the DBS punch and the quantitation of target analytes.

229 Bio-Rad QX200 ddPCR system provides absolute quantitation of target DNA molecules using fluorescent d

230 ction-based ELISA principle (ReactELISA) for quantitation of the carbon nucleophilic lipid metabolite

231 Direct quantitation of the degree of contingency in succession

232 limited error, which allows for the relative quantitation of the diastereomeric pairs without all sta

233 -ray Spectroscopy (STEM-EDS) mapping provide quantitation of the extent of Ag and Pt replacement as a

234 Strikingly, quantitation of the ion atmosphere content indicates tha

235 et enabling the identification and sensitive quantitation of the microcystin (MC) congeners.

236 This work establishes that direct quantitation of the nucleotide-bound conformation is req

237 ates strongly and significantly with ex vivo quantitation of the pro-inflammatory biomarker myelopero

238 Quantitation of the serum concentration of 25-hydroxyvit

239 The detection and quantitation of the target is enabled by the use of a un

240 inition of Method Operable Design Region and quantitation of the total amount of phenols in millet sa

241 cally-uniform wild-type population for later quantitation of their distinct muscle gene expression, a

242 ll ultimately allow sensitive and systematic quantitation of these chromosome segregation defects in

243 of TSG101-UEV concentration, allowed precise quantitation of these interactions.

244 AT and PRA-derived products and the relative quantitation of total linoleate oxidation products with

245 synthesized for real-time visualization and quantitation of TPP dynamics within live cells at the su

246 Quantitation of tricuspid regurgitant (TR) severity can

247 CP-MAS NMR spectroscopy, providing the first quantitation of yields in COF postsynthetic modification

248 f detection of ~200 attomoles and a limit of quantitation of ~ 390 attomoles.

249 Here we report a highly parallel protein quantitation platform integrating nanoparticle (NP) prot

250 suitable for qualitative detection, accurate quantitation proved difficult.

251 g) were evaluated using several metrics: SUV quantitation, qualitative image quality, and lesion dete

252 191 copies/ml (BKV), and the lower limit of quantitation ranged from 442 international units (IU)/ml

253 Quantitation relies on assumptions as to the state of th

254 nsures that fragmentation is consistent when quantitation relies on the ratio of the target to the in

255 ns of CMV disease but standardization of CMV quantitation remains problematic, mostly due to differen

256 The limits of detection or quantitation resulted in excellent sensitivities (range

257 n hardly provide direct comparative glycomic quantitation results.

258 he limit of detection (S/N > 3) and limit of quantitation (S/N > 10) were determined as 0.025 and 0.1

259 Comparative relative quantitation showed differences in protein abundance of

260 Results: Our analysis of volume-of-interest quantitation showed that SUVs remain stable down to 1/3

261 his error did not affect numeric results, as quantitation shown in the paper was carried out with thr

262 Quantitation stability of a (68)Ge flood phantom was dem

263 ps to an external, certified, small-molecule quantitation standard.

264 e shotgun proteomics for protein and peptide quantitation, statistically analyzed normalized spectral

265 le throughput was ca. 15 samples/h, with PAH quantitation successful at microgram per kilogram levels

266 s well as linear dynamic range and limits of quantitation suitable for determining neurochemicals at

267 ere AssayR provides more accurate and robust quantitation than XCMS, and we propose that tailored pea

268 lled iTop-Q (intelligent Top-down Proteomics Quantitation), that automatically performs large-scale p

269 ncentrations beyond the assay upper limit of quantitation (ULOQ), resulting in isotope dilution facto

270 ected for parallel reaction monitoring (PRM) quantitation using a high-resolution Orbitrap instrument

271 by performing proteome-wide differential PTM quantitation using linear models analysis (limma).

272 ata processing, followed by verification and quantitation using parallel reaction monitoring (PRM).

273 ms and glycoconjugates can be monitored with quantitation using this protocol.

274 Quantitation validation was tested on glycans released f

275 The quantitation variations in our analytical procedures wer

276 or (SKIE), a pipeline automating Ki-67 index quantitation via whole-slide image (WSI) analysis and (2

277 ction was 0.175 meq.O(2)/kg and the limit of quantitation was 0.35 meq.O(2)/kg.

278 shed for the assay while the linear range of quantitation was 10(2)-10(5) CFU/ml.

279 Quantitation was based on proteins for which heat-stable

280 wavelength 277nm and 289nm respectively and quantitation was carried out by first order derivative m

281 Quantitation was done using highly selective and sensiti

282 PET stability metrics demonstrated that PET quantitation was not affected during simultaneous aggres

283 or ionization efficiency of FC, the limit of quantitation was sufficient for precise and accurate qua

284 The sum of 14 polyphenols (HPLC quantitation) was positively correlated with the mean ai

285 crease proteome coverage and improve protein quantitation, we optimized cell fractionation and sample

286 ess than 5% RSD, and limits of detection and quantitation were 0.1-2.1 and 0.4-7.0 mug L(-1), respect

287 Limits of detection and quantitation were 1.0 and 3.3 ng g(-1), respectively.

288 The limits of detection and quantitation were 1.2 +/- 0.1, 2.1 +/- 0.1, and 2.7 +/-

289 n 0.03 and 0.17 ng/mL, whereas the limits of quantitation were between 0.09 and 0.52 ng/mL, indicatin

290 ery was 115% and the limits of detection and quantitation were in the order of the parts per trillion

291 7 who had >=1 subsequent echocardiogram with quantitation were included.

292 est that uncertainty is introduced into SVOC quantitation when internal standards are not added promp

293 es this issue, it is not amenable to precise quantitation, which limits its utility for differential

294 For simpler and improved quantitation with ESI-MS, laser-induced fluorescent imag

295 eins to enable their sensitive detection and quantitation with ICP MS.

296 high sensitivity and reproducibility of PTM quantitation with levels as low as 1.0%.

297 In addition, quantitation with minimally processed rat plasma samples

298 minarily screened and submitted for absolute quantitation with NGP-based stable-isotope-labeled (SID)

299 High accuracy of quantitation within a dynamic range of 2 orders of magni

300 o yields compound-independent F response for quantitation without compound-specific standards.