ライフサイエンスコーパス: read through

1 r therapeutics: exon skipping and stop codon read through.

2 ic and bacterial cells results in stop-codon read through.

3 ame extent as the effects on [PSI+]-mediated read-through.

4 depletion of TIA-1 by siRNA increased (pA)p read-through.

5 of the [PSI+] prion to cause nonsense-codon read-through.

6 minimal sequence necessary for translational read-through.

7 st increase in frame shifting and stop codon read-through.

8 se drugs, as more substrate is available for read-through.

9 82X mutation, CFTR1281, without the need for read-through.

10 e ade1-14 selective marker for translational read-through.

11 (+)] prion state leads to more translational read-through.

12 e expression and drugs for nonsense mutation read-through.

13 ras are functional outcomes of transcription read-through.

14 vation as a potent enhancer of transcription read-through.

15 f the 91 unique viral sequences annotated as read-through, 90% had one of six of the 64 possible codo

16 y assessing the ability of RNA polymerase to read through a factor-dependent terminator.

17 transcribed the antitermination sequence to read through a Rho-dependent terminator.

18 construction of highly accurate preassembled reads through a directed acyclic graph-based consensus p

19 dependent transcription of the tcpA promoter reads through a proposed terminator between the tcpF and

20 e found in linear time of the lengths of the reads through a well-designed suffix tree structure.

21 requires special translational cofactors to "read-through" a UGA-stop codon that specifies SEC incorp

22 Here, we demonstrate translational read-through across two evolutionarily conserved, in-fra

23 compound RTC14 did not result in significant read-through activity in vivo and demonstrated the level

24 ough, suggesting that these factors modulate read-through activity.

25 osome-binding antibiotic with translational 'read-through' activity, efficiently targeted abnormal WN

26 with the classical aminoglycoside antibiotic read-through agent geneticin (G418) across a diverse ran

27 le PTC124 (Ataluren) has been described as a read-through agent, capable of suppressing premature ter

28 rmination factor, which increases stop codon read-through allowing ribosomes to translate into the 3-

29 transcriptional interference resulting from read through and dual strand transcription.

30 highlight a novel link between transcription read-through and aberrant expression of oncogenes and ch

31 3' end of exon 14, leading to the subsequent read-through and capture of formerly intronic sequence a

32 ly extended proteins and elevated stop codon read-through and frameshift events.

33 ngle-component RNA viruses use translational read-through and frameshift mechanisms to down-regulate

34 lded reverse transcriptases with both robust read-through and high mutation rates at m(1)A sites.

35 Overexpression of Sup35 decreases stop codon read-through and rescues oxidant tolerance consistent wi

36 f-of-concept study to demonstrate successful read-through and restoration of RP2 function for the R12

37 elation between codon-specific translational read-through and the activity of a 120-kDa RNA-binding p

38 between the missense mutation caused by the read-through and the structural context of the protein.

39 dicated small amounts of native ATP7A(R201X) read-through and were associated with a dramatic clinica

40 to overcome RDEB PTC mutations by inducing "read-through" and incorporation of an amino acid at the

41 en reading frames, extensive transcriptional read-through, and numerous unpredicted RNA start sites h

42 econdary structures may cause frameshifting, read-through, and/or recoding of the multiple stop codon

43 heterochromatin and RNAi factors to suppress read-through antisense transcripts.

44 hile G418 exhibits varying activity in every read-through assay, there is no evidence of activity for

45 Using a sensitive terminator read-through assay, we identified trans-acting Terminato

46 It retarded termination and increased read-through at Rho-dependent terminators, even in the a

47 The [PSI+] state causes read-through at stop codons and can lead to phenotypic v

48 eatment with THZ1 suppressed RNA polymerase 'read through' at the end of the last exon, which resembl

49 nome Atlas (TCGA) reveals that transcription read-through beyond the termination site is a source of

50 on factor TFIIS enables RNA polymerase II to read through blocks to elongation in vitro and interacts

51 strongly inhibit [PSI+]-mediated stop codon read-through but do not cure cells of the [PSI+] prion.

52 ble of generating functional proteins is the read-through, but the outcome of read-through products i

53 ted region of the luciferase gene stimulated read-through by 6-11-fold in selenium-replete cells; abs

54 Promotion of read-through by MoMLV RNase H prevents nonsense-mediated

55 FCA reduces transcriptional read-through by promoting proximal polyadenylation at ma

56 wo mRNAs are linked due to a small amount of read-through by RNA polymerase.

57 on elongation complexes and facilitate pause read-through by stabilizing RNAP in a posttranslocated r

58 ocess of nonsense suppression (translational read-through) by ribosomes, making it difficult to deter

59 rial metabolism, was selected as a potential read-through candidate based on evolutionary conservatio

60 ability of hnRNP H mutations to suppress the read-through caused by an SR protein mutation suggests t

61 novel class of circular transcripts, termed read-through circRNAs, that involved exons originating f

62 The ability of the cellular machinery to read through click-linked DNA was further probed by usin

63 NMD also limits the efficacy of read-through compound (RTC)-based therapies.

64 We also show how the read-through compound ataluren (PTC124) increases PPT1 e

65 or testing nonsense suppression therapies or read through compounds in CLN3 disease in the future.

66 ent stem cells, and show that small molecule read-through compounds, designed to induce read-through

67 Two such read-through compounds, RTC13 and RTC14, were recently i

68 n testing nonsense suppression therapies and read-through compounds.

69 The relative efficiency of these read-through contexts in mammalian tissue culture cells

70 lls lacking RP2 indicates that translational read-through could be clinically beneficial for patients

71 ing replication from base mismatches and can read through damaged bases, such as abasic sites and thy

72 MTCH2 read-through-deficient cells, generated using CRISPR-Cas

73 n which the maximal efficiency of stop codon read-through depends on the interaction between MoMLV RT

74 The degree of read-through-derived peptide presentation varies with th

75 ng shows that structural features within the read-through domain of delta 159 RNA are less well deter

76 type RNA, whereas predicted structure in the read-through domain of evolved pseudorevertant delta 549

77 re less well determined than they are in the read-through domain of wild-type RNA, whereas predicted

78 scription elongation complex is competent to read through downstream termination signals.

79 h NUT RNA, which, in turn, causes failure to read through downstream termination sites.

80 loop termed boxB, enabling RNA polymerase to read through downstream terminators processively.

81 acologic NMD inhibition combined with a PTC "read-through" drug led to restoration of full-length p53

82 Treatment with read-through drugs also leads to increased protein funct

83 e experiments demonstrate that translational read-through drugs are able to suppress the Mecp2 p.R294

84 We have also shown how the read-through drugs gentamicin and ataluren (PTC124) incr

85 phin are specifically targeted by stop codon read-through drugs, whereas out-of-frame deletions and i

86 The amber codon is thus read through during translation at apparently high effic

87 ontain an in-frame amber (UAG) codon that is read through during translation.

88 Two independent observers (O.A., P.K.) read through each article and classified the articles ac

89 interaction with the ribosome influence PTC read-through efficiency.

90 old by placing the murine leukemia virus UAG read-through element upstream of the first UGA codon or

91 1-beta isoform was derived from exon 2 and a read-through event of intron 2.

92 omosomal rearrangements, and transcriptional read through events.

93 AHL signaling, QrpR, and transcriptional read-through events integrate to ensure AHL-dependent ex

94 TC mutations in different contexts exhibited read-through expression of ATM fragments, with three of

95 chanical coupling tuned to ensure an optimal read-through frequency.

96 of acpB appears to occur via transcriptional read-through from atxA-dependent start sites 5' of capB.

97 quences in some T-DNA constructs, transcript read-through from selectable markers is also possible, w

98 al activation in response to cellobiose, but read-through from the celA promoter contributes to expre

99 CFTR proteins that are poorly functional and read-through, full-length products.

100 quences, relieving repression and increasing read-through, hasA transcription, and capsule production

101 lates RNA transcript cleavage and polymerase read-through have been well characterized, its in vivo r

102 g events such as frameshifting or stop-codon read-through have occurred, elucidating alternative tran

103 orders, yet small molecules that promote PTC read-through have yielded mixed performance in clinical

104 e gas sensors show an optic response that is read through high-resolution digital color detectors.

105 ragment of DNA polymerase I, Sequenase could read through homopolymeric regions with more than five T

106 uced, normally rare, and naturally unstable "read-through" human acetylcholinesterase variant, AChE-R

107 d rate of -1 programmed ribosomal frameshift read-through in a dual-luciferase assay for observing tr

108 ifferentiation-dependent polyadenylation and read-through in HPV-31.

109 rs lead to an 11-fold increase in terminator read-through in in vitro transcription reactions.

110 nes, most of which result in transcriptional read-through in protein-coding genes.

111 G418 increased read-through in selenium-replete cells as well as in the

112 e caused by a higher frequency of stop codon read-through in these species than in yeast, possibly be

113 al decrease in termination and the resulting read-through increases full-length product formation.

114 ng control closely correlated with increased read-through, indicating that a functional NRS is necess

115 Using the translational read-through inducing drugs (TRIDs) G418 and PTC124 (Ata

116 Translational read-through inducing drugs (TRIDs), such as PTC124, wer

117 therapeutic potential of the nonsense codon read-through-inducing drug, PTC124, in treating PXE.

118 s the ability of the viral RNA polymerase to read through intergenic junctions.

119 ERV long terminal repeat (LTR) sequences and read-through into known gene sequences.

120 tion sequences allowed significant levels of read-through into the late region in undifferentiated ce

121 ination of transcription in order to prevent read-through into the next gene, which could possibly di

122 A level, which appears to result from longer read-through into the telomere downstream of the active

123 evels, which determine whether transcription reads through into the mgtA coding region or stops withi

124 However, extensive transcriptional read-through invalidated similar correlations at later t

125 e of the recoding signals known to stimulate read-through is a hexanucleotide sequence of the form CA

126 This 'read-through' is in competition with termination and is

127 ICL influences which polymerases are able to read through it.

128 ransgene robustly silenced transcription and read through its polyadenylation sequence.

129 To effect terminator read-through, lambdaQ must gain access to RNA polymerase

130 s fused to TMOF at the C terminus by using a read-through, leaky stop codon that facilitated expressi

131 n recurrent nonsense mutation, p.R1141X, the read-through may result in substitution of the arginine

132 transcript by a hitherto unrecognized intron read-through mechanism.

133 we have directly tested the transcriptional read-through mechanism.

134 In addition, events such as polymerase read-throughs, mis-mapping due to gene homology, and fus

135 anscriptome sequencing approach, we observed read-through mRNA chimeras, tissue-type restricted chime

136 ad aligner capable of handling multi-mapping reads, through new and compact index structures that red

137 To thoroughly test the ability of PTC124 to read through nonsense mutations, we conducted a detailed

138 lts are consistent with a mechanism in which read through of a pseudouridylated stop codon in bacteri

139 26S proteolytic activity leads to increased read through of a transcription termination site.

140 to EutX and, instead, causes transcriptional read through of multiple eut genes.

141 p codons, and one was predicted to result in read through of the normal translational termination cod

142 e phenotypic effects of [PSI+] may be due to read-through of "normal" stop codons, thereby producing

143 reading frame which exploits transcriptional read-through of a minimal polyadenylation signal from a

144 Here, we identify and characterize native read-through of a nonsense mutation (R201X) in the human

145 notypes, but many do not appear to be due to read-through of a single stop codon, but instead are mul

146 ucleosome repositioning, and transcriptional read-through of associated DNA.

147 nd identified multiple peptides derived from read-through of conventional stop codons.

148 n Mn2+, in vitro activation of transcription read-through of mgtA by Mn2+ is much greater than by Ca2

149 e read-through compounds, designed to induce read-through of mRNA around premature termination codons

150 all traits tested involved [PSI(+)]-mediated read-through of nonsense codons.

151 Thus, our results suggest that read-through of nonsense mutations in ABCC6 by PTC124 ma

152 Molecules that induce ribosomal read-through of nonsense mutations in mRNA and allow pro

153 the [PSI] genetic element that enhances the read-through of nonsense mutations in the yeast Saccharo

154 The ability of this drug to facilitate read-through of nonsense mutations was examined in HEK29

155 ecific mutations or drugs developed to allow read-through of nonsense mutations, whereas other therap

156 n of polyadenylation at (pA)p, necessary for read-through of P41-generated capsid gene pre-mRNAs whic

157 In the absence of stalling, read-through of poly(A) produces a poly-lysine tag, whic

158 Aminoglycoside antibiotics facilitate read-through of premature stop codons in prokayotes and

159 e of their capacity to enhance translational read-through of premature termination codons (PTCs), the

160 of the T-box anti-terminated state allowing read-through of regulated genes.

161 tion as its depletion causes transcriptional read-through of selected gene terminators and because it

162 the mother cell location are inferred to be read-through of spoIIIG, the structural gene for sigma(G

163 acy and tolerability of a drug which induces read-through of stop codons in Duchenne muscular dystrop

164 Sup35 adopts the prion state, [PSI(+)], the read-through of stop codons increases, uncovering hidden

165 s of polyadenylation factor crosslinking and read-through of termination sequences.

166 of an amber suppressor tRNA should result in read-through of the 326 open reading frames (ORFs) that

167 is reinforced by experiments indicating that read-through of the CTS can be efficiently promoted by s

168 eved by aminoglycoside-induced translational read-through of the E375X premature stop codon, restorin

169 units, which may contribute to the increased read-through of the early sequence.

170 RNA polymerase II to the ATG start site and read-through of the ET-1 coding region.

171 sion either by ribosomal frameshifting or by read-through of the gag stop codon.

172 merged into a single ORF (termed a mORF) by read-through of the intervening stop codon, and may comp

173 Read-through of the missing codon occurred only when the

174 NP1 is required for read-through of the MVC internal polyadenylation site an

175 P1 is required for both the splicing and the read-through of the proximal polyadenylation site of the

176 tream of genes to act as a backup in case of read-through of the real stop codon.

177 n of therapeutics for SMA designed to induce read-through of the SMNDelta7 stop codon to show increas

178 IIsnR), and CPL4RNAi plants showed increased read-through of the snRNA 3'-end processing signal, lead

179 idine (Psi) allows efficient recognition and read-through of these stop codons by a transfer RNA (tRN

180 We also demonstrate unexpected read-through of transcription at the Rho-independent ter

181 romising therapeutic target that would allow read-through of transcripts to enhance protein function

182 ral delivery of the missing dystrophin gene, read-through of translation stop codons, exon skipping t

183 s rrn nut-like site enhances transcriptional read-through of untranslated RNA consistent with an anti

184 eam edge of the transcription bubble lead to read-through of various types of pauses and termination

185 r translational cofactors are necessary for "read-through" of a UGA stop codon that specifies selenoc

186 A codon into a reporter construct allows for read-through only in the presence of selenium.

187 ft mutation, these two parts formed a single read-through open reading frame (ORF).

188 ORF9A and ORF9 mRNAs, whereas expression of read-through ORF9A/9/10 and ORF9/10 transcripts was incr

189 A-AAV RNAs generated from upstream promoters read through (pA)p, as seen for AAV2.

190 hat encompass the NRS also lead to increased read-through past the viral polyadenylation site, sugges

191 olymerase (RNAP), thus helping the enzyme to read through pausing and arresting sites on DNA.

192 is such that at physiological pH the active, read-through permissive conformation is populated at app

193 significant variability in their stop codon read-through phenotypes depending on the background geno

194 The level of this read-through product is proportional to CAG repeat lengt

195 These read-through products did not influence the efficacy and

196 eins is the read-through, but the outcome of read-through products is highly variable depending on th

197 tations revealed potential identities of the read-through products.

198 protein, the only known, naturally occurring read-through protein in eukaryotes, was sequenced by ion

199 In contrast, only the read-through protein is functional with E60X- and G542X-

200 The rabbit beta-globin read-through protein, the only known, naturally occurrin

201 Single-channel studies of the read-through proteins of E60X- and G542X-CFTR demonstrat

202 ion results in both C-terminus truncated and read-through proteins that are partially or fully functi

203 ation codons can be ignored to obtain larger read-through proteins.

204 drug amlexanox was tested for its ability to read-through PTC mutations in cells derived from patient

205 reading frames and extensive transcriptional read-through resulting in overlapping mRNAs.

206 essation or reinitiation of translation, and read-through, resulting in differential effects on prote

207 thway leading to lambdaQ-mediated terminator read-through results in the formation of a highly stable

208 iple bound proteins on single RNA strands by read-through reverse transcription and DNA sequencing.

209 e observations also suggest that the lengthy read-through RNAs postulated to be intermediates in retr

210 Stop codon read-through (SCR) is a process of continuation of trans

211 ints specific for individual alleles and can read through sequences previously not accessible for ana

212 AT/Met treatment decreased selenium-mediated read-through significantly (p < 0.001) in luciferase con

213 In some cases, the transcriptional read-through significantly reduced expression of the ass

214 y more functional protein referred to as SMN read-through (SMN(RT)).

215 lots detect normal PAI transcripts and dsRNA read-through species, but not diced smRNAs, suggesting t

216 ligation gives a urea linkage with excellent read-through speed, or a squaramide linkage that is read

217 rom read-pair, read-depth and one end mapped reads through statistical likelihoods based on Poisson f

218 actor IIS provided roughly the same level of read-through stimulation for transcript elongation in th

219 Ribosomes can read through stop codons in a regulated manner, elongati

220 the 64 possible codons immediately 3' of the read-through stop codon.

221 Aminoglycosides also induced stop codon read-through, suggesting that these antibiotics alleviat

222 on region significantly reduced the level of read-through, suggesting that these factors modulate rea

223 me DNA synthesis and, in a reaction we call "read-through synthesis," forks established while the sub

224 mance by re-learning eye-movement control in reading through systematic oculomotor practice.

225 Transcripts which read through the +93 site quantitatively terminate at a

226 h polymerase failed to terminate and instead read through the gene-end sequence to generate a bicistr

227 The failure of RNA polymerase to read through the mutation also reduces the abundance of

228 linacin-3 gene had induced a frameshift that read through the original stop codon and allowed the chi

229 most polymerases are unable to recognize and read through the presence of a single caging group on th

230 e that Escherichia coli RNAP can effectively read through the site-specific DNA-binding proteins in v

231 ing frame once or twice in a -1 direction to read through the stop codon in the gag reading frame.

232 mplexes that terminate but not by those that read through the terminator.

233 ch as ataluren, target these transcripts and read-through the PTC, leading to the production of a ful

234 onic mRNA and resulted instead in polymerase reading through the gene junction to produce a bicistron

235 on originating at a distal upstream site and reading through the hurR-bhuR intergenic region contribu

236 GRO-Seq analysis showed the polymerase reading through the termination signal in the downstream

237 s are transcribed by RNA polymerase II which reads through the region containing early polyadenylatio

238 ions and compete with DNA polymerase V which reads through the tandem lesion.

239 y to cryptic epitopes revealed by stop codon read-through therapies and potentially other therapeutic

240 o be a good model for aminoglycoside-induced read-through therapy.

241 ients by using aminoglycosides to induce PTC read-through, thereby restoring levels of full-length AT

242 The read-through transcript contributes to total w transcrip

243 ed by several assays, as was a less abundant read-through transcript encompassing pilA1, pilA2, and p

244 hus, as is the case in Escherichia coli, the read-through transcript from rpsO-pnp is cleaved by RNas

245 Read-through transcript levels also significantly increa

246 RNA substrate representing a portion of the read-through transcript normally produced in S. coelicol

247 -untranslated leader segment of a trp operon read-through transcript, it can disrupt a large secondar

248 be the site for RNase IIIS processing of the read-through transcript.

249 titermination increases the synthesis of the read-through transcript.

250 cistronic gene clusters in L. major leads to read through transcription and increased expression of d

251 n defects affect gene expression and whether read through transcription is detrimental to cell growth

252 ression by enabling RNA polymerase (RNAP) to read through transcription terminators preceding bacteri

253 ied vector splice sites, we reduced residual read-through transcription and demonstrated an effective

254 e CID from Rtt103 (Nrd1(CID(Rtt103))) causes read-through transcription at many genes, but can also t

255 that increases the elongation rate increases read-through transcription at Sen1-mediated terminators.

256 s and, by binding to the same site, prevents read-through transcription from the distal, lmo1569 prom

257 in sea and sak transcription was a result of read-through transcription from upstream latent phage pr

258 Here we show that read-through transcription from yeast small nucleolar RN

259 at inefficiently terminates HasS, permitting read-through transcription of hasABC, and a putative pro

260 proportion of crtJ expression is promoted by read-through transcription of orf192 (aerR) transcripts

261 0463 could be increased TcdR translation and read-through transcription of the tcdA and tcdB genes.

262 or can activate the transcription or lead to read-through transcription of their downstream genes.

263 rm at any DNA sequence but are suppressed by read-through transcription or that they can overlap the

264 We observed a surprising abundance of read-through transcription originating outside and insid

265 ed viral RNAs and demonstrate that low level read-through transcription produces a novel class of chi

266 hese transcripts and that spreading requires read-through transcription, as well as slicing by Argona

267 mRNA, caused,most likely, by light-mediated read-through transcription.

268 minators are excluded from functional TUs by read-through transcriptional interference, while antisen

269 e precise boundaries of many mRNAs including read-through transcripts and location of mRNA start site

270 In contrast, few M-F read-through transcripts are detected in SV-infected cel

271 nction is ineffective; a large number of M-F read-through transcripts are produced.

272 s of polyadenylated histone mRNA and nascent read-through transcripts at the histone locus.

273 We show that Hox gene read-through transcripts can be spliced to produce inter

274 A stability, mRNA processing, and removal of read-through transcripts in S. pyogenes.

275 Rider full-length and read-through transcripts past the typical transcription

276 oundaries of lowly expressed unannotated and read-through transcripts putatively encoding fusion gene

277 Abundant read-through transcripts were observed in the presence o

278 rids (R-loops), including from antisense and read-through transcripts, in a nusG missense mutant defe

279 as well as in the rapid degradation of rnpB read-through transcripts.

280 3-kb RNA spanning ORF10 only and three other read-through transcripts.

281 t stages of meiosis, including antisense and read-through transcripts.

282 with rSVhMFjCG produced an abundance of M-F read-through transcripts; this result indicated that the

283 An alternative outcome, read-through translation (or nonsense suppression), is w

284 eBCR complexes that may arise as a result of read-through translation across premature Ter5 stop codo

285 hly sensitive sandwich Western blot revealed read-through translation of Igh (Ter5H) message, indicat

286 ells from patients with RDEB that respond to read-through treatment.

287 a dcl4 mutant, the resulting transcriptional read-through triggers an RNA interference-mediated gene

288 Read-through type VII collagen was readily detectable in

289 corporating the missense variant erroneously read through UAG and UGA stop codons of mRNAs.

290 [PSI (+)] prion, empowers yeast ribosomes to read-through UGA stop codons.

291 rough speed, or a squaramide linkage that is read-through under selective conditions.

292 nfluence on the ability of the polymerase to read through uracil and hypoxanthine, the same kinetic p

293 they resulted from extensive transcriptional read-through, use of downstream polyadenylation sites, a

294 The usually rare read-through variant of acetylcholinesterase (AChE-R) is

295 ons up to 20 mug ml(-1), and the facilitated read-through varied not only with dose but also with seq

296 Moreover, we identified a read-through w transcript initiated from a retrotranspos

297 Upon differentiation, this read-through was increased by approximately 50%, indicat

298 eosome in the absence of tails, but complete read-through was not substantially increased by tail rem

299 antly, the suppression of selenium-dependent read-through was similar whether an SV40 promoter or the

300 adjacent active chromatin or transcriptional read-through, which may be free of selective biases.