ライフサイエンスコーパス: reagent

1 e precursor into an oxygen-free chalcogenide reagent.

2 are a new class of trifluoromethylthiolating reagent.

3 ronic acids and the thioimidate KAT transfer reagent.

4 ccurred between nitrite and the added Griess reagent.

5 reagent to deplete the excess amount of TMT reagent.

6 0%), irrespective of the size of the hydride reagent.

7 und to be a single-electron process for both reagents.

8 molecules, aiding the development of future reagents.

9 on-based test uses simple and stable organic reagents.

10 voiding any on-paper interaction between the reagents.

11 s to provide unsymmetrical 3-boryl-1,4-diene reagents.

12 ncorporation and unavailability of precursor reagents.

13 mal models and may lead to clinically useful reagents.

14 erties were compared to those of standard DA reagents.

15 ious methodology and limited availability of reagents.

16 to the diminished reactivity of the involved reagents.

17 th alpha-halocarbonyl compounds and arylzinc reagents.

18 and antifluorescein antibodies as detection reagents.

19 stability and were sensitive to DNA-damaging reagents.

20 r high throughput and reduced consumption of reagents.

21 ed times lower than that of the existing PDT reagents.

22 because of high reactivity of organometallic reagents.

23 y of well-established chemical reactions and reagents.

24 te experimental and therapeutic use of these reagents.

25 unctionalization or covalent modification of reagents.

26 , photocontrollability, and widely available reagents.

27 rained acyclic systems as radical allylation reagents.

28 ically requires harsh reaction conditions or reagents.

29 as to limit any on-paper interaction between reagents.

30 corporation of halide salts in organolithium reagents.

31 en allylmagnesium halides and other Grignard reagents.

32 ion of enones mediated by hypervalent iodine reagents.

33 ously unreactive toward addition of alkyl-Cr reagents.

34 manufacture practices using solely xeno-free reagents.

35 to the engineered enzyme and click chemistry reagents.

36 abilizes and separates a pair of Diels-Alder reagents.

37 pecific IgE when tested using native protein reagents.

38 solvents, metal catalysts, energy, and redox reagents.

39 ith a generic biotinylated antihuman capture reagent, (2) "on-bead" digestion with IdeS and/or PNGase

40 ntaining CRISPR-based nucleic acid detection reagents(7) self-organize in a microwell array(8) to pai

41 y to the general behavior of ion-interaction reagents (a broader term than ion-pairing reagent), sign

42 steps and simplifies the system to a single reagent addition step.

43 ivity can easily be quantified by sequential reagent additions in 96- or 384-well plates, making it r

44 Here, we present a reagent-agnostic automated SARS-CoV-2 testing platform t

45 n real-time with small amounts of sample and reagents, allowing for fast detection of deadly toxins w

46 Fine-tuning of the EBX reagents allows optimization of their reactivity and phy

47 on of only 1 equiv of boron from the diboron reagent and a requirement for neat substrate.

48 was complexed with a commercial transfection reagent and administrated using different routes in type

49 anion serving as the visible-light-absorbing reagent and electron and hydrogen atom donor to promote

50 was the generation in situ of an aza-Nazarov reagent and its subsequent intramolecular aza-Michael cy

51 Reagent and sample consumptions are greatly reduced to m

52 r both hydrogen atom abstraction from the CH reagent and the selective formation of the C-O coupling

53 n alternative is to introduce genome editing reagents and a homologous recombination (HR) donor templ

54 ategies that employ stoichiometric hazardous reagents and are associated with difficulty in purificat

55 enter cells without the aid of transfection reagents and can detect, track, and quantify analytes in

56 We provide outlines of traditional reagents and chemical procedures for the preparation of

57 cleosides by coupling aryl or alkyl Grignard reagents and halogenated purine nucleosides in the prese

58 ire only inexpensive, commercially available reagents and hardware commonly found in a routine pathol

59 We applied class II tetramer reagents and mass cytometry to investigate the ontogeny

60 Issues pertaining to reagents and mechanisms are discussed.

61 orial treatment of different ICIs with other reagents and modalities.

62 upling processes to alternative nucleophilic reagents and novel main group approaches.

63 pared gamma,gamma-disubstituted allyldiboron reagents and proceed in the presence of a fluoride activ

64 matically affecting the availability of test reagents and requiring laboratories to implement multipl

65 efficiency significantly reduce the cost of reagents and sequencing.

66 ogies make use of large volumes of hazardous reagents and solvents, as well as energy-intensive proce

67 However, the need for several reagents and steps and the high cost of PCR hinder its w

68 urify, and characterize a panel of six Yariv reagents and suggest approaches that could be valuable f

69 ith nucleophilic reagents including Grignard reagents and tetrabutylammonium cyanide.

70 The origami approach allows to add reagents and trigger the sequential reactions in separat

71 cular biologist using commercially available reagents and typically gives rise to 10-30 unique sybodi

72 in population-screening assays, high-quality reagents and well-characterized antigens and antibodies

73 ide in an acid medium, with reduction of the reagents and without the digestion step in the sample pr

74 y uses an hypervalent iodine(III)-CF(2)CF(3) reagent, and mechanistic investigations are consistent w

75 caine, the Dess-Martin periodinane oxidation reagent, and the fluorinating agent AlkylFluor.

76 require the use of stoichiometric activating reagents, and avoids the use of explosive reagents or to

77 mass-tag reagents, nonpolymer-based mass-tag reagents, and inorganic nanoparticles.

78 h tert-alkyl organometallic or -alkyl halide reagents, and it enables the expedient formation of quat

79 ctive in the method, including pH, volume of reagents, and sample volume were optimized.

80 l-defined protocols for obtaining pure Yariv reagents, and their complete compound characterization d

81 tly instrumentations, laboratory setups, and reagents, and they are invasive.

82 80 degrees C, a range of detergents, Trizol reagents, and UV energies were successful at inactivatin

83 NNs) that enter cells without a transfection reagent are used to target Plk1 mRNA in primary cells fr

84 Further, many of these reagents are challenging to handle, engage in undesired

85 Bespoke reagents are commonly used because of issues associated

86 Developmental regulators and gene-editing reagents are delivered to somatic cells of whole plants.

87 reafter, the future perspectives of mass-tag reagents are discussed considering the current state-of-

88 ons, strengths, and shortcomings of mass-tag reagents are highlighted, summarized, and discussed, wit

89 The process consists of two parts where reagents are mixed together, choosing from one type of a

90 eaction times, and temperatures, whereby the reagents are passed through a copper coil reactor.

91 control (e.g. heat or light) - through which reagents are passed.

92 Mass cytometry reagents are probes tagged with metal isotopes of define

93 itro XL-MS studies at the systems-level, new reagents are still needed to help expand PPI coverage.

94 These reagents are synthesized by diazo coupling between phlor

95 Chiral 1,2-bimetallic reagents are useful motifs in synthetic chemistry.

96 mulas for the linker adducts formed by these reagents are usually deduced from expert knowledge and t

97 Togni's benziodoxole-based reagents are widely used in trifluoromethylation reactio

98 ycosylations promoted by triflate-generating reagents are widespread synthetic methods for the constr

99 ipyridine, or using the excess of the >P(O)H reagent as the P-ligand.

100 ichiometric amounts of oxidizing or reducing reagents as well as electron transfer events by electric

101 he way to the addition of N-H bonds of other reagents, as well as O-H and C-H bonds, across unactivat

102 ernative to this textbook description: these reagents behave as aluminium nucleophiles.

103 conditions, uses ubiquitous and inexpensive reagents, boasts a wide scope, and can be made highly at

104 characteristics to existing tandem mass tag reagents but with increased fragmentation efficiency and

105 the traditional cross-coupling with Grignard reagents, but also including a diverse range of organic

106 esult from activation of CoBr(2) by Grignard reagents, but the operative mechanism and identity of th

107 as established as a single electron transfer reagent by treating it with an acceptor molecule tetracy

108 and effectively replaces harsh fluorinating reagents by a combination of oxalyl chloride and potassi

109 Malibu-Glo reagent can be conveniently produced in mammalian cells

110 pernatant containing the secreted Malibu-Glo reagent can directly be used for detection of cell surfa

111 d, calling cards, and demonstrate that these reagents can be delivered to the live mouse brain and us

112 tational algorithms to design genome editing reagents can mitigate off-target edits in plants.

113 ndly chemical delivery system containing all reagents (catalyst, ligand, and base) for three importan

114 mine bonds decorate the skeleton of numerous reagents, catalysts, and organic materials.

115 arge source to generate radical fluoranthene reagent cations.

116 es, which aim at identifying the most proper reagent combination in only three distinct steps.

117 peratures, reagent excess, and air-sensitive reagents common among the existing protocols.

118 t of magnetic beads between small volumes of reagents commonly employed for NA extraction and purific

119 Moreover, miniaturization reduced reagent consumption and residue generation.

120 offers a 25-50-fold reduction of sample and reagent consumption with up to 50 technical replicates p

121 ism rules as time, cost, sample requirement, reagent consumption, energy requirements, and production

122 These reagents-containing HPMC capsules are easy to prepare, c

123 ly exclusive pairs of bioorthogonal labeling reagents continues to drive the design of new compounds

124 adioactivity are expensive, both in terms of reagent cost and disposal.

125 tical to scale, as miniaturization decreases reagent cost per test by more than 300-fold.

126 es upon current single diagnostics by saving reagents, costs, time, and labor.

127 genomic characterization, further improving reagent design and application.

128 as drug screening platform, particularly for reagents designed to increase foetal haemoglobin express

129 The reagent di-tert-butyl ethynylimidodicarbonate is demonst

130 I) with 1,4-phenylenedipropionic acid (PDPA) reagent dianions generated via electrospray ionization (

131 s assay is inexpensive using "off the shelf" reagents, does not require centrifugation or mechanical

132 mentation costs and logistical problems with reagents during the global SARS-CoV-2 pandemic have hind

133 Here, we report the first derivatization reagent, (E)-2-cyano-N-(2-(2,5-dioxo-2,5-dihydro-1H-pyrr

134 th a wide variety of enones and allyldiboron reagents efficiently to deliver ketone products that con

135 anoparticles without the need for additional reagents, enabling otherwise difficult functional groups

136 p-dimethylaminobenzaldehyde (p-DMAB) as the reagent entrapped within polymer network.

137 y the addition of the water-miscible organic reagent ethylene glycol, which radically alters the prop

138 ds the transition metals, high temperatures, reagent excess, and air-sensitive reagents common among

139 However, performing efficient reagent exchange on chip for large numbers of embryos re

140 These reagents exhibit sensitivity to the conformational integ

141 The maleimide group is a widely used reagent for bioconjugation of peptides, proteins, and ol

142 elenazolone as a novel bifunctional selenide reagent for both off- and on-DNA C-H selenylation under

143 he nanobody is demonstrated to be a powerful reagent for C3 detection in immunohistochemistry and flo

144 PPN) salt, is shown herein to be a versatile reagent for nucleophile tetraphosphorylation.

145 Singlet oxygen is a versatile reagent for the selective oxidation of organic compounds

146 -CoV-2 antibodies will be useful as valuable reagents for accessing the antigenicity of vaccine candi

147 trate the use of biocatalysis to supply such reagents for automated synthesis.

148 proved bioavailability, making them serve as reagents for bacterial imaging and killing.

149 ased format, and utilizes the same precursor reagents for complex formation with each of the explosiv

150 logic assays using newly designed SARS-CoV-2 reagents for detecting the presence of receptor-binding

151 discussed, with special emphasis on mass-tag reagents for MCB.

152 A new class of cationic ion-interaction reagents for reversed-phase chromatography is introduced

153 s/interferences from matrix, 2) load/release reagents for the bio-assay, 3) reduce the operating task

154 we report the use of diazirines as practical reagents for the decarboxylative amination of simple and

155 osphorus Incorporation (PI, abbreviated Pai) reagents for the modular, scalable, and stereospecific s

156 enges facing this target were examined for 7 reagents found in commercially available mouth rinses an

157 The free-radical-activated glycan-sequencing reagent (FRAGS) selectively conjugates to the unique red

158 butyrylcholinesterase activity, delivering a reagent free analytical tool.

159 h was then utilized as the sensing layer for reagent-free electrochemical AST.

160 high analytical frequency, the low cost and reagent-free nature of this method demonstrate its poten

161 ion process mediated by a hypervalent iodine reagent from an inexpensive phenol containing a lactate

162 Isobutane as a reagent gas in combination with both low-resolution (LR)

163 de addition with the developed E-crotylboron reagent gave (E)-anti-1,2-oxaborinan-3-enes with excelle

164 The amounts and toxicity of reagents, generated waste, energy requirements, the numb

165 rategy based on positively charged hydrazine reagent (Girard's reagent P) coupled with matrix-assiste

166 Many different types of mass-tag reagents have been developed to afford myriad applicatio

167 Many reagents have emerged to study the function of specific

168 These reagents have similar characteristics to existing tandem

169 etic application of the well-known aminating reagent hydroxylamine-O-sulfonic acid (HOSA) has been ex

170 s the treatment of plasmonic excitation as a reagent in chemical reactions; the chemical potential of

171 d after photochemical charging and used as a reagent in dark reactions, such as the reduction of meth

172 cular assemblies to be considered a standard reagent in organic synthesis they must first demonstrate

173 hile PPh(3) (2 equiv) reacts with the chloro reagent in toluene to give the expected iminophosphorane

174 3))-rich and sterically hindered alkyl boron reagents in a practical and modular manner.

175 When expressed as genetically encoded reagents in cells, these monobodies potently block necro

176 Photoisomerising these reagents in living cells allows optical control over mic

177 Diazomethane is one of the most versatile reagents in organic synthesis, but its utility is limite

178 erivatives have been established as powerful reagents in organic transformations, but so far only a h

179 icious coupling partners and 2-pyridyl boron reagents in particular are notorious for their instabili

180 the electrode and the catalysts or chemical reagents in solution.

181 n of membrane proteins and are indispensable reagents in structural biology.

182 heir derivatives are some of the most useful reagents in the chemical sciences(1), with applications

183 of expensive catalysts, starting from simple reagents, in good to excellent yields and with complete

184 anometallic compounds, for example, Grignard reagents, in the presence of a major excess of tetrameth

185 de and subsequent coupling with nucleophilic reagents including Grignard reagents and tetrabutylammon

186 advantages over existing cysteine alkylation reagents, including accelerated reaction rates, improved

187 ce in organic solvents and are driven by the reagents' interaction with bulky chiral catalysts.

188 Here, we introduced genome editing reagents into single-cell bovine embryos to compare the

189 he correction function is independent of the reagent ion used.

190 between HF and Na(2)A(NaA)(n)(+), gas-phase reagent ions produced by nano-ESI where A represents the

191 pon APCI, hydride abstraction by carbocation reagent ions, is not correct.

192 g energy of cluster ions between analyte and reagent ions, that is needed for the assessment of a cho

193 al reactions; the chemical potential of this reagent is tunable by the light intensity.

194 yclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosi

195 ng replication networks in pools of chemical reagents is a key challenge in systems chemistry.

196 e accessibility of our reference strains and reagents is relevant to the development of new therapeut

197 l biochemical assays which require expensive reagents, is time-consuming and more often is only indic

198 nserved sex-specific splicing mechanisms and reagents, it has the potential to be adaptable to other

199 by the single-stage synthesis of commercial reagent (known as Baran aminating reagent precursor) fro

200 such as the requirement of specific capture reagents, limited mass resolution of low mass change met

201 lyzed by the QD through self-assembly of the reagent molecules on the QD surface, but these experimen

202 Since the fluorescent reagent N-(1-pyrene)iodoacetamide was first used to labe

203 Analogously, the reagent (N,N,N')-tri-Boc 2-ethynylhydrazine serves as a

204 co-staining PC-3 cells with a Golgi staining reagent, NBD C(6) -ceramide, NIR imaging in the Golgi ap

205 whole blood sample, filter paper to load the reagents needed for the measurement, and office paper to

206 these compounds into polymer-based mass-tag reagents, nonpolymer-based mass-tag reagents, and inorga

207 viously described air-sensitive diorganozinc reagents of limited availability to easily handled and w

208 s, which has hampered the development of new reagents of the same family.

209 Nonpolymer-based mass-tag reagents offer multiple applications: cell detection, ce

210 ed glycans via removal of excess of labeling reagents often leads to sample losses.

211 mpurities or unreacted/incompletely utilized reagents or catalysts.

212 ng reagents, and avoids the use of explosive reagents or toxic gases, such as CO, as the C1 synthon.

213 ons of [1.1.1]propellane with organometallic reagents, organoboronic esters, and a variety of electro

214 sitively charged hydrazine reagent (Girard's reagent P) coupled with matrix-assisted laser desorption

215 s at removing the cytotoxic elements of each reagent, permitting accurate determination of levels of

216 esidues in native polypeptides, which uses a reagent platform based on the P(V) oxidation state.

217 Well-characterized antibody reagents play a key role in the reproducibility of resea

218 commercial reagent (known as Baran aminating reagent precursor) from isobutyric acid and N-hydroxysuc

219 ) with the commercially available Lawesson's reagent produced sulfur-enriched-reduced GO (S-rGO).

220 r diagnostic laboratory was not equipped for reagent production, we took advantage of space and perso

221 These reagents provide a foundation for investigations into vi

222 can be produced within 1 h without elaborate reagent purification.

223 s deeply into innate signaling, obstacles of reagent purity may be a cause of some of the discrepanci

224 ative fragmentation using hypervalent iodine reagents, reactions of donor-acceptor cyclopropanols, an

225 ange from uniform to core-shell, affected by reagent reactivity and solvent choice.

226 opyridine N-oxide as an oxygen atom transfer reagent reverses the regioselectivity to give 5-amino-1,

227 As such, the bifunctional allylboron reagent serves as a useful linchpin to join two distinct

228 urthermore, a skin prick test using the same reagent showed a positive result, and the reaction incre

229 on reagents (a broader term than ion-pairing reagent), significant (up to 5-fold) and consistent enha

230 own to be effective cationic ion-interaction reagents, significantly enhancing the retention of organ

231 ability and concentrations of skin test (ST) reagents, ST and drug provocation test (DPT) protocols,

232 paper-based sensor provides the functions of reagent storage, sample transportation, and nucleic acid

233 opically enriched functional groups into the reagent structure, labeling sites can be more easily ide

234 The addition of an electron-deficient reagent such as 2,6-dichloro-1,4-benzoquinone suppressed

235 t without the necessity of adding additional reagents such as acids or bases that can perturb biologi

236 editing is typically performed by delivering reagents such as Cas9 and single guide RNAs to explants

237 T-PCR tests has been extremely slow, and key reagents such as PCR primers and RNA extraction kits are

238 globin tests involve the use of highly toxic reagents such as potassium cyanide, the polymerization-b

239 nd 2) a dependence on hazardous fluorinating reagents such as XeF(2) .

240 r supplemental TNF ligand or a cross-linking reagent, suggesting that the antibody agonist by itself

241 reaction, devoid of sensitive organometallic reagents, takes advantage of an in situ generated chiral

242 ed here is an inexpensive and easily handled reagent that enables the development of simple and scala

243 t consolidating this chemical as a new green reagent that will be widely used in fine chemistry, gree

244 Given the wide array of inactivation reagents that are being used in laboratories during this

245 of chemical crosslinking and the removal of reagents that are incompatible with mass spectrometry.

246 , the identification of functional groups or reagents that are mutually reactive with one another, bu

247 Reagents that bind tightly and specifically to biomolecu

248 Reagents that disrupt the interaction of TL1A with DR3 t

249 Recent studies have described acylimidazole reagents that react in high yields at 2'-OH groups stoch

250 sulting Pd-protein OACs are stable, storable reagents that retain biological activity and can be used

251 quire filtration for sterilization, and used reagents that were available from commercial suppliers.

252 of deuterated and proteated acid and hydride reagents, the deuterated positions on the cyclohexene ri

253 In contrast to simple organolithium reagents, the monomeric THF-solvate was found to be more

254 om temperature and employs readily available reagents; the mild conditions allowed the use of highly

255 by means of the reaction with the organozinc reagents, thereby allowing for the synthesis of cyclic a

256 toms are proposed to direct the fluorinating reagent through C-H hydrogen bonds.

257 additive during the TMT labeling as a decoy reagent to deplete the excess amount of TMT reagent.

258 d lowering the concentration of a multi-role reagent to effect quantitative dissociation and subseque

259 alpha-vinyl substituent of the allylboronate reagent to occupy a pseudo-axial position in the transit

260 The addition of an organometallic reagent to the commercially available sulfur dioxide sur

261 2) surface area, reduces the total volume of reagents to <10 muL, and requires only two sample transf

262 Additions of Grignard reagents to aldehydes or methyl ketones provided no evid

263 ew describes the additions of allylmagnesium reagents to carbonyl compounds and to imines, focusing o

264 ed differences in the reactivity of antibody reagents to cIPV and sIPV products.

265 ple procedure using commonly available shelf reagents to deliver organoselenides that, to our knowled

266 We demonstrate the use of these reagents to deplete a set of 103 proteins individually i

267 The use of charge-reducing reagents to generate lower-charge ions has gained popula

268 By choosing suitable nucleophilic reagents to selectively capture these intermediates, bot

269 to synthesize SH probes, while providing new reagents to study protein depalmitoylation.

270 enetic system and reporter virus provide key reagents to study SARS-CoV-2 and develop countermeasures

271 Delivery of the editing reagents to WAS HSPCs led to full rescue of WASp express

272 nzene-1,3,5-tricarboxylate (BTC(3-) ) ligand reagents, to form MOF nanocrystals, and collect and char

273 as a single phosphorus anion (P(-)) transfer reagent towards metal carbonyls and a chlorogermylene co

274 nsitivity issue, we have developed a generic reagent, ultratargeted two-dimensional liquid chromatogr

275 ne N-oxide using dioxazolone as an amidating reagent under mild conditions is disclosed.

276 In the presence of carbonyl compounds, these reagents undergo highly diastereoselective allylations t

277 All reagents used are more easily accessible and cost-effect

278 ociated with disposable laboratory gloves or reagents used during sample preparation was investigated

279 Despite the importance of these reagents, very little is known about the reaction mechan

280 s and alpha-2 degrees amines into alkylating reagents via Katritzky pyridinium salts.

281 tion parameters (temperature, time, milk and reagent volume) are followed.

282 Our platform uses small reagent volumes and inexpensive equipment, paving the wa

283 ion or are cumbersome to operate, need large reagent volumes, and are costly, limiting their usabilit

284 The novel reagent was prepared on 200-g scale in a single step, re

285 This reagent was then used to assemble two biologically relev

286 ive to expensive commercial isobaric tagging reagents, we developed our own custom N,N-dimethylleucin

287 fy testing and reduce the volume of required reagents, we devised a multiplex RT-qPCR assay to detect

288 (0) and Ni(II) with 2,2'-bipyridine or the P-reagents were in accord with our mechanistic proposition

289 r consumption, and only 10 muL of sample and reagents were required.

290 ), and other electric-field types, as "smart reagents", which enable in principle control over wide-r

291 secondary allylic carbonates with benzylzinc reagents, which are prepared from widely available benzy

292 ariety of ketones and allylic 1,1-diboronate reagents, which enables the efficient preparation of div

293 tion (RT-LAMP) assay compatible with current reagents, which utilizes a colorimetric readout in as li

294 y are less dependent on high quality binding reagents, while a key limitation is the relatively lower

295 harp contrast, bulk reactions using the same reagents with a catalyst yield exclusively C-alkylation

296 ts allow for the manipulation of samples and reagents with an unprecedented precision and control, pa

297 er (SET) in the reactions of organomagnesium reagents with carbonyl compounds.

298 are a new class of cationic ion-interaction reagents with clear advantages over the currently employ

299 ndary benzyl halides and secondary alkylzinc reagents with internal alkenes, which furnishes products

300 us that the P-C coupling reaction of >P(O)H reagents with PhX (X = I and Br) in the presence of NiCl